Standard name
Human Ortholog
Description Ubiquitin-binding component of the Rsp5p E3-ubiquitin ligase complex; disruption causes temperature-sensitive growth, overexpression causes missorting of amino acid permeases; BUL1 has a paralog, BUL2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0.05 0 0 0 0 0.05 – 0.07 0 0 0 0.08 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0.05 0.05 0.12 0.13 0.19 0 0 0 0 0 0
Cytoplasm 0.91 0.93 0.95 0.86 0.94 0.88 0.84 0.81 0.7 – 0.69 0.62 0.98 0.99 0.98 0.93 0.88 0.85 0.78 0.82 0.72 0.81 0.81 0.77
Endoplasmic Reticulum 0.06 0.07 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.19 0 0.22 0.29 0.28 0.46 – 0.47 0.53 0 0 0 0 0 0 0 0 0.13 0.05 0 0.06
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 2 2 1 2 1 – 0 1 0 0 0 2 1 1 3 1 2 0 1 3
Bud 0 1 3 9 3 5 6 6 9 – 16 4 1 3 14 1 0 1 0 0 1 1 3 2
Bud Neck 0 0 1 1 0 0 0 1 2 – 0 1 0 0 0 0 1 0 2 2 5 3 1 2
Bud Site 0 0 0 0 0 0 0 1 1 – 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 9 1 2 2 4 1 7 – 8 6 7 8 10 13 15 16 0 1 4 0 1 0
Cytoplasm 43 62 213 152 171 199 234 294 127 – 169 167 159 151 178 101 100 72 72 88 172 113 124 146
Endoplasmic Reticulum 3 5 1 0 0 3 1 1 3 – 0 4 0 0 0 4 7 6 1 1 5 3 2 5
Endosome 0 0 1 4 4 2 1 4 0 – 0 0 1 0 0 0 1 0 2 2 2 1 6 5
Golgi 0 0 0 0 1 0 0 0 0 – 0 0 0 0 0 0 1 0 1 0 2 2 2 0
Mitochondria 0 2 4 34 8 51 80 101 84 – 114 144 0 2 6 1 1 3 2 2 31 6 3 11
Nucleus 0 0 0 0 0 1 0 3 0 – 0 0 0 1 1 1 3 1 0 0 0 0 1 0
Nuclear Periphery 0 0 1 0 0 0 0 0 0 – 0 2 0 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 1 1 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 1 1 0 0 0 2 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 1 2 0 0 1 3 0 – 1 2 1 0 0 3 1 1 2 0 1 0 0 1
Vac/Vac Membrane 0 0 0 0 0 0 6 2 0 – 4 4 0 0 0 0 4 2 0 5 5 3 3 3
Unique Cell Count 47 67 224 176 181 227 280 363 182 244 270 162 152 182 109 114 85 93 108 241 140 155 189
Labelled Cell Count 48 70 235 204 191 265 334 421 234 313 336 170 165 210 126 135 103 93 108 241 140 155 189


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.0 5.6 4.6 4.6 5.0 4.7 4.1 4.2 3.7 – 3.8 3.4 6.0 5.8 5.1 7.0 8.0 8.1 6.0 6.4 6.9
Std Deviation (1e-4) 0.6 0.9 1.3 1.0 1.3 1.4 1.1 0.9 1.0 – 1.3 1.1 1.7 1.6 1.4 0.9 1.7 1.6 1.0 1.9 2.1
Intensity Change (Log2) – – – -0.02 0.1 0.02 -0.18 -0.15 -0.31 – -0.3 -0.43 0.36 0.32 0.13 0.59 0.78 0.81 0.37 0.47 0.58


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 3.2 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0.1 0.6 0.7 2.7 3.1 4.3
Cytoplasm -3.1 -0.3 -2.8 -4.1 -4.8 -6.9 – -7.2 -8.7 1.6 2.3 1.4 -0.9 -2.4 -3.0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 1.6 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.9431 3.1547 3.2507 2.7343 3.1634 3.2332 3.4984 4.0093 3.8362 3.7024 3.7685 3.8242 4.6415 5.0242 4.3991 5.0491 5.9318 4.793
Actin 0.0433 0.0065 0.0392 0.0082 0.0427 0.0252 0.0491 0.0056 0.0157 0.0049 0.0276 0.007 0.0839 0.0063 0.0313 0.1002 0.1047 0.0056
Bud 0.0006 0.0008 0.0033 0.0004 0.0006 0.0025 0.0015 0.0045 0.0014 0.0025 0.0026 0.0016 0.0008 0.0026 0.0013 0.0011 0.0039 0.001
Bud Neck 0.0014 0.0023 0.0168 0.0036 0.0012 0.0225 0.0103 0.0015 0.0029 0.0017 0.0014 0.0106 0.0015 0.0017 0.006 0.1163 0.0031 0.0026
Bud Periphery 0.0009 0.0007 0.0047 0.0004 0.001 0.0071 0.0021 0.0039 0.0015 0.0044 0.0059 0.0076 0.001 0.0022 0.0009 0.0013 0.0034 0.0018
Bud Site 0.0032 0.0452 0.0393 0.001 0.0015 0.0037 0.0154 0.0693 0.0202 0.0062 0.0017 0.0004 0.009 0.0421 0.0059 0.0062 0.0159 0.0004
Cell Periphery 0.0028 0.0053 0.005 0.0008 0.0017 0.0024 0.003 0.0026 0.0012 0.0009 0.0004 0.001 0.0024 0.0024 0.0023 0.002 0.0014 0.0008
Cytoplasm 0.5362 0.7511 0.6253 0.7918 0.677 0.7628 0.5337 0.7734 0.7009 0.7348 0.5353 0.7655 0.6638 0.7165 0.7507 0.5916 0.5967 0.7694
Cytoplasmic Foci 0.0537 0.0512 0.0714 0.0844 0.014 0.0561 0.0649 0.0374 0.0649 0.0303 0.103 0.0553 0.0659 0.0497 0.0427 0.0352 0.0541 0.0406
Eisosomes 0.0006 0.0001 0.0005 0.0001 0.0005 0.0002 0.0003 0.0001 0.0001 0.0001 0.0002 0.0001 0.0004 0.0001 0.0002 0.0002 0.0004 0
Endoplasmic Reticulum 0.0197 0.0061 0.0055 0.0111 0.0132 0.0081 0.0237 0.0067 0.0059 0.0054 0.0037 0.0058 0.007 0.0028 0.0049 0.0047 0.0034 0.0048
Endosome 0.0753 0.0191 0.0395 0.0467 0.0147 0.027 0.0614 0.0128 0.0349 0.0368 0.0501 0.028 0.0406 0.0171 0.0416 0.0318 0.0149 0.0284
Golgi 0.0216 0.006 0.0154 0.0062 0.0051 0.0141 0.0261 0.005 0.0206 0.0337 0.0383 0.0096 0.0156 0.0047 0.0049 0.0035 0.0091 0.0041
Lipid Particles 0.0229 0.0058 0.0086 0.004 0.0058 0.0044 0.0174 0.0022 0.0033 0.042 0.0358 0.0013 0.0148 0.0006 0.0027 0.0025 0.0035 0.0014
Mitochondria 0.0276 0.0004 0.0172 0.0007 0.0458 0.0097 0.0057 0.0015 0.0066 0.033 0.094 0.0122 0.003 0.0002 0.0013 0.0005 0.0034 0.0006
None 0.1126 0.0907 0.0866 0.0315 0.0477 0.0284 0.1245 0.0622 0.1002 0.045 0.0773 0.0888 0.0721 0.142 0.0889 0.095 0.1667 0.135
Nuclear Periphery 0.0315 0.0007 0.0009 0.0009 0.0523 0.0014 0.008 0.0006 0.0019 0.0006 0.0004 0.0006 0.0003 0.0003 0.0007 0.0004 0.0017 0.0002
Nucleolus 0.0008 0.0002 0.0008 0.0001 0.0004 0.0005 0.0003 0.0007 0.0002 0.0002 0.0001 0 0.0002 0.0003 0.0005 0.0002 0.0002 0
Nucleus 0.0082 0.001 0.0011 0.0008 0.021 0.0022 0.015 0.0021 0.0018 0.0006 0.0003 0.0005 0.0004 0.0013 0.0011 0.0006 0.0008 0.0003
Peroxisomes 0.0144 0.0008 0.0093 0.0021 0.0395 0.0126 0.027 0.0011 0.0122 0.0111 0.0173 0.0016 0.0139 0.0021 0.0068 0.0006 0.0087 0.0004
Punctate Nuclear 0.0087 0.0006 0.0015 0.0013 0.0012 0.001 0.0021 0.0025 0.0006 0.0004 0.0009 0.0007 0.0006 0.0004 0.001 0.0004 0.002 0.0002
Vacuole 0.0083 0.0051 0.0073 0.0032 0.0069 0.0064 0.0058 0.004 0.0027 0.0047 0.0028 0.0013 0.0027 0.0045 0.0038 0.0053 0.0014 0.0019
Vacuole Periphery 0.0056 0.0003 0.001 0.0006 0.0062 0.002 0.0027 0.0003 0.0005 0.0009 0.0009 0.0005 0.0003 0.0001 0.0004 0.0004 0.0005 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.2583 18.5093 21.2263 27.294 20.7441 27.0901 25.2649 30.0851 26.1337 26.7594
Translational Efficiency 0.7445 0.7492 0.7355 0.505 0.5627 0.5577 0.5577 0.4812 0.5982 0.5588

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
132 49 403 682 423 284 1313 407 555 333 1716 1089

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 698.19 834.58 992.43 834.93 741.78 819.12 951.89 833.49 731.41 821.39 961.41 834.39
Standard Deviation 80.33 101.18 107.87 111.43 125.21 109.42 113.28 103.62 117.59 108.38 113.35 108.58
Intensity Change Log 2 — 0.257431 0.507346 0.258036 — 0.143083 0.359803 0.168174 — 0.199659 0.433227 0.212444

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000655 0.000885 0.006345 0.002610 0.000864 0.000759 0.000680 0.000340 0.000814 0.000777 0.002010 0.001761
Bud Neck 0.041950 0.017597 0.009033 0.011061 0.028533 0.039920 0.004996 0.005376 0.031724 0.036635 0.005944 0.008936
Bud Site 0.041161 0.022688 0.038478 0.054414 0.021872 0.033375 0.017660 0.019621 0.026459 0.031803 0.022549 0.041411
Cell Periphery 0.000361 0.000290 0.000310 0.000168 0.000352 0.000176 0.000162 0.000165 0.000354 0.000193 0.000197 0.000167
Cytoplasm 0.460835* 0.370322* 0.313803* 0.401231* 0.406249* 0.280449* 0.503656* 0.427562* 0.419231* 0.293674* 0.459069* 0.411072*
Cytoplasmic Foci 0.280763* 0.319412* 0.181856* 0.058645 0.319807* 0.298889* 0.074740 0.082377 0.310521* 0.301909* 0.099896 0.067515
Eisosomes 0.000545 0.000209 0.000159 0.000077 0.000517 0.000218 0.000080 0.000089 0.000524 0.000216 0.000098 0.000082
Endoplasmic Reticulum 0.000536 0.000871 0.006664 0.001569 0.000712 0.000488 0.002422 0.000917 0.000670 0.000544 0.003418 0.001326
Endosome 0.028148 0.050500 0.020876 0.021472 0.041284 0.082034 0.020307 0.018968 0.038160 0.077394 0.020441 0.020536
Golgi 0.010116 0.004844 0.001412 0.016136 0.011279 0.009734 0.000376 0.004022 0.011002 0.009014 0.000619 0.011608
Lipid Particles 0.003343 0.004922 0.011440 0.003769 0.007063 0.003285 0.004768 0.005159 0.006178 0.003526 0.006335 0.004288
Mitochondria 0.012471 0.003134 0.000320 0.014800 0.004233 0.005716 0.000393 0.005524 0.006192 0.005336 0.000375 0.011333
Mitotic Spindle 0.000309 0.000780 0.044853 0.061044 0.003365 0.006352 0.023522 0.019416 0.002638 0.005532 0.028532 0.045486
None 0.000263 0.000175 0.001165 0.005985 0.015105 0.001939 0.002173 0.003415 0.011575 0.001680 0.001936 0.005024
Nuclear Periphery 0.000050 0.000218 0.001190 0.000898 0.000170 0.000163 0.001168 0.000546 0.000142 0.000171 0.001173 0.000766
Nuclear Periphery Foci 0.000149 0.000712 0.013919 0.002178 0.000511 0.000391 0.005083 0.001440 0.000425 0.000438 0.007158 0.001902
Nucleolus 0.000330 0.000676 0.000389 0.000359 0.001737 0.001667 0.000712 0.000803 0.001402 0.001521 0.000636 0.000524
Nucleus 0.024944 0.021864 0.094553 0.052082 0.024235 0.023539 0.091893 0.064406 0.024403 0.023293 0.092518 0.056688
Peroxisomes 0.012810 0.029581 0.021465 0.007484 0.013037 0.019859 0.004177 0.002330 0.012983 0.021289 0.008237 0.005558
Vacuole 0.073283 0.148907 0.231499* 0.271922* 0.097452 0.188376 0.240543* 0.325819* 0.091704 0.182568 0.238419* 0.292066*
Vacuole Periphery 0.006978 0.001414 0.000272 0.012096 0.001623 0.002671 0.000489 0.011705 0.002897 0.002486 0.000438 0.011950

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.89 -4.39 -0.97 -0.19 3.95 0.57 1.04 2.50 1.87 2.17 0.26 -3.46 0.28 0.08 3.17
Bud Neck 2.94 4.97 5.90 2.02 2.99 -1.99 8.59 8.82 7.22 1.48 -0.90 9.84 10.14 7.26 1.90
Bud Site 2.21 0.93 2.09 -0.40 1.82 -2.13 3.71 2.97 3.72 0.09 -1.02 3.24 1.87 2.27 -0.82
Cell Periphery 0.78 0.85 4.30 2.30 11.21 3.07 4.00 4.29 2.31 1.59 3.37 4.05 5.68 3.66 8.36
Cytoplasm 1.99 6.98 5.23 0.79 -2.83 6.81 -0.26 3.38 -3.25 4.19 7.39 2.88 5.82 -2.92 3.94
Cytoplasmic Foci -1.10 6.45 13.94 9.90 13.47 1.45 23.53 22.80 19.01 0.18 0.76 23.60 28.32 23.01 10.11
Eisosomes 2.81 3.35 4.12 3.94 9.51 5.05 7.83 7.70 5.54 -1.00 5.74 8.40 8.87 6.91 5.67
Endoplasmic Reticulum -0.81 -11.78 -4.09 -1.65 10.25 1.46 -11.02 -3.51 -5.50 8.66 0.82 -15.69 -5.26 -6.14 11.97
Endosome -1.68 1.47 3.56 3.65 5.21 -5.05 6.82 7.77 9.61 2.14 -5.50 6.77 9.33 10.57 4.86
Golgi 1.49 2.78 1.59 0.09 -2.45 0.73 8.23 6.08 3.54 -1.63 1.02 8.22 4.77 2.68 -3.18
Lipid Particles -0.83 -7.48 1.69 1.54 8.68 2.72 1.81 2.11 0.15 1.10 2.41 0.02 3.15 1.47 5.38
Mitochondria 1.30 1.75 1.17 -0.49 -3.10 -0.43 2.02 0.88 1.22 -1.70 0.34 2.65 1.12 0.71 -3.50
Mitotic Spindle -1.85 -6.30 -6.76 -6.62 2.00 -0.83 -5.52 -2.44 -1.70 1.13 -0.97 -8.04 -6.08 -4.50 0.87
None 1.18 -3.51 -2.77 -2.85 -2.04 2.52 2.69 2.55 -0.20 -0.71 2.45 2.63 2.20 -0.86 -1.78
Nuclear Periphery -1.72 -13.22 -14.90 -8.79 0.83 -0.08 -18.92 -9.55 -10.36 9.60 -1.02 -22.46 -15.54 -15.26 6.01
Nuclear Periphery Foci -3.06 -9.59 -3.27 -1.78 8.43 0.82 -8.53 -5.77 -6.66 6.77 -0.45 -12.54 -3.85 -3.70 9.72
Nucleolus -0.76 -1.68 0.38 0.90 3.73 -0.04 1.07 1.30 1.36 0.97 -0.22 0.99 1.50 1.64 3.15
Nucleus 0.42 -8.57 -9.41 -10.01 1.59 -0.19 -17.54 -9.04 -8.68 6.39 -0.08 -19.51 -13.37 -12.79 5.63
Peroxisomes -1.48 -1.01 4.54 2.48 5.23 -2.52 6.81 8.81 7.30 3.69 -2.93 4.79 9.30 7.17 5.48
Vacuole -3.16 -14.57 -25.52 -10.72 -9.75 -7.34 -24.40 -21.82 -13.80 -7.14 -8.23 -28.16 -33.37 -18.59 -11.07
Vacuole Periphery 1.26 1.51 0.78 -1.74 -2.80 -2.05 5.85 -1.43 -0.96 -2.04 0.42 2.41 -0.66 -1.30 -3.35
Standard name
Human Ortholog
Description Ubiquitin-binding component of the Rsp5p E3-ubiquitin ligase complex; disruption causes temperature-sensitive growth, overexpression causes missorting of amino acid permeases; BUL1 has a paralog, BUL2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (39%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Bul1

Bul1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bul1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available