Standard name
Human Ortholog
Description Carboxy-terminal domain (CTD) phosphatase; essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p); relocalizes to the cytosol in response to hypoxia
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0.07 0.11 0.11 0.09 0 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.07 0 0 0 0.08 0.1 0.06 0.23 0.21 0.21 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.98 0.93 0.97 0.94 0.94 0.92 0.91 0.92 0.91 0.91 0.9 0.88 0.91 0.86 0.86 0.86 0.9 0.87 0.95 0.94 0.96 0.84 0.71 0.72
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0.07 0.14 0.09 0.1 0 0 0 0 0 0 0 0 0 0 0.06 0.07
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.06 0.15 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 0 2 0 0 0 1 0 1 0 2 1 0 0 0 0 0 0 1 1 1 0 4 2
Bud Neck 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 8 3 6 4 2 7 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 15 3 11 13 7 11 10 5 4 6 12 19 48 51 30 7 18 0 4 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 1 0 2 0 0 0 0 0 0 0 1 5 2 2 0 0 0 1 2 0
Endosome 0 0 0 2 0 0 0 1 1 0 0 0 1 1 1 2 0 3 2 0 1 1 4 3
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 1 0 0 0 2 1
Mitochondria 1 29 0 3 4 21 40 18 64 55 53 27 0 2 0 2 2 8 0 4 2 0 4 4
Nucleus 217 378 287 228 228 237 365 264 257 239 227 276 256 378 397 303 187 211 215 387 290 97 204 197
Nuclear Periphery 0 1 0 0 2 0 2 0 0 1 0 2 1 8 2 4 0 0 0 0 0 0 0 1
Nucleolus 1 8 0 4 1 5 13 6 20 36 24 32 10 7 12 4 6 6 1 0 0 3 18 20
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 1 3 6 0 0 1 0 0 0 1 0 1
SpindlePole 0 1 2 1 1 1 8 4 1 1 0 4 0 2 2 0 0 1 0 2 0 0 2 2
Vac/Vac Membrane 1 8 3 1 0 2 1 4 1 0 0 3 4 7 4 16 8 8 2 8 6 6 42 32
Unique Cell Count 222 408 295 243 242 258 400 286 281 262 253 315 282 437 463 352 208 243 227 414 303 116 288 274
Labelled Cell Count 222 440 298 251 250 274 451 310 356 340 314 364 292 456 476 368 212 258 227 414 303 116 288 274


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.8 6.4 7.3 6.9 7.3 5.8 6.0 6.4 5.2 5.2 5.2 6.1 8.1 8.4 7.9 11.8 12.7 11.4 7.2 7.4 7.9
Std Deviation (1e-4) 1.1 1.2 1.0 1.6 1.7 1.2 1.2 1.4 1.3 1.2 1.2 1.4 2.1 2.7 2.1 2.8 2.8 3.1 1.6 1.8 1.7
Intensity Change (Log2) – – – -0.08 -0.0 -0.31 -0.28 -0.19 -0.47 -0.47 -0.48 -0.26 0.16 0.21 0.11 0.7 0.8 0.65 -0.01 0.03 0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.5 3.0 0 1.6 2.0 0 0 0 2.2 3.6 5.2 5.2 4.3 0 3.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 5.0 5.6 4.4 8.7 8.3 8.3 5.1 0 0 0 0 0 0
Nucleus -2.0 -1.8 -2.9 -3.3 -2.7 -3.1 -3.1 -3.7 -4.5 -3.3 -5.0 -5.2 -5.0 -3.5 -4.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 3.1 0 4.7 6.6 5.4 5.6 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 2.6 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.5454 2.6934 2.6161 1.8255 2.3613 2.3008 3.8074 4.197 4.1316 3.5595 4.4105 3.8214 3.2929 3.3428 3.03 3.1922 2.9863 3.1378
Actin 0.0097 0 0.005 0.0003 0.0004 0.008 0.0177 0 0.0146 0.006 0.0844 0.0068 0.0149 0.0003 0.0043 0.0229 0 0
Bud 0.0052 0.0008 0.0002 0.0024 0.0011 0.0011 0.0007 0 0.0004 0.0022 0.0025 0.0003 0.0004 0.0001 0.0001 0.0002 0 0
Bud Neck 0.0029 0.0002 0.0002 0.0007 0.0009 0.0072 0.005 0.0001 0.0003 0.0041 0.0019 0.0007 0.0068 0.0002 0.0001 0.0001 0.0001 0.0006
Bud Periphery 0.0109 0.0005 0.0002 0.0032 0.0062 0.002 0.0011 0 0.0008 0.0039 0.0031 0.0004 0.0005 0.0002 0.0002 0.0001 0 0
Bud Site 0.0068 0.0013 0.0006 0.0023 0.0006 0.0008 0.0074 0.0001 0.0018 0.0133 0.0115 0.0004 0.0068 0.0005 0.0003 0.0005 0 0
Cell Periphery 0.0013 0 0.0001 0.0006 0.0027 0.0002 0.0005 0 0.0003 0.0008 0.0005 0 0.0002 0.0001 0.0001 0 0 0
Cytoplasm 0.0087 0.0002 0.0072 0.0049 0.0003 0.0011 0.0051 0 0.003 0.0012 0.0083 0.0002 0.0069 0.0004 0.0019 0.0013 0 0.0005
Cytoplasmic Foci 0.0169 0.0002 0.0034 0.0018 0.0001 0.0041 0.0645 0.0021 0.0057 0.0149 0.0308 0.0006 0.0108 0.0002 0.0019 0.0202 0 0.0001
Eisosomes 0.0001 0 0.0001 0.0001 0 0 0.0001 0 0.0007 0.0002 0.0004 0 0.0001 0 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0086 0 0.0041 0.0015 0.0012 0.0008 0.0035 0 0.0023 0.0018 0.0164 0.0001 0.0027 0.0001 0.002 0.0006 0 0.0001
Endosome 0.0265 0 0.0074 0.0005 0.0011 0.0057 0.0704 0.0024 0.0151 0.0529 0.1127 0.0014 0.0371 0.001 0.0023 0.0204 0 0.0003
Golgi 0.0046 0 0.0036 0.0001 0.0001 0.0024 0.0228 0.0021 0.0067 0.0168 0.0692 0.0009 0.0163 0.0003 0.0032 0.0279 0 0
Lipid Particles 0.0203 0.0001 0.0046 0.0008 0.0002 0.003 0.0227 0.0016 0.0104 0.0209 0.0537 0.0005 0.026 0.0005 0.0052 0.0035 0 0
Mitochondria 0.0076 0.0002 0.0211 0.0007 0.0108 0.0029 0.0125 0.0003 0.0081 0.0258 0.0867 0.001 0.0088 0.003 0.0207 0.0026 0.0007 0.0001
None 0.0229 0.0001 0.0119 0.0351 0.0001 0.0004 0.0016 0 0.002 0.0006 0.0021 0.0001 0.0093 0 0.0019 0.0004 0 0
Nuclear Periphery 0.0523 0.0026 0.0126 0.0118 0.0036 0.0134 0.012 0.0005 0.0047 0.0039 0.0467 0.0005 0.0489 0.0036 0.0039 0.0003 0.0018 0.0048
Nucleolus 0.0207 0.0145 0.0171 0.0233 0.0483 0.0367 0.0199 0.0072 0.0041 0.0041 0.01 0.0291 0.0064 0.0088 0.0043 0.0025 0.0251 0.0197
Nucleus 0.7314 0.9668 0.8915 0.886 0.8813 0.881 0.6926 0.9823 0.91 0.7967 0.4236 0.9535 0.7643 0.9782 0.9396 0.8862 0.9711 0.9636
Peroxisomes 0.0126 0 0.0025 0.0002 0.0001 0.0152 0.0188 0.0006 0.0018 0.0088 0.0119 0.0012 0.0035 0.0001 0.0064 0.0097 0 0
Punctate Nuclear 0.0134 0.0118 0.0019 0.0212 0.0003 0.0015 0.0084 0.0005 0.0009 0.0018 0.0067 0.002 0.021 0.001 0.0007 0.0003 0.0009 0.01
Vacuole 0.0126 0.0003 0.0033 0.002 0.0237 0.0049 0.0102 0.0001 0.0043 0.0149 0.0075 0.0002 0.0032 0.0009 0.0004 0.0001 0.0001 0.0001
Vacuole Periphery 0.0041 0.0002 0.0015 0.0005 0.017 0.0076 0.0025 0.0001 0.002 0.0046 0.0096 0.0001 0.0052 0.0004 0.0004 0.0002 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 37.3823 26.9134 20.3038 27.7017 35.7816 24.5734 26.9853 25.2537 25.965 34.9467
Translational Efficiency 0.7191 0.8765 0.8939 0.8204 0.6651 0.8876 0.8088 0.6115 0.6673 0.5783

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1635 441 228 1207 2021 1601 1906 932 3656 2042 2134 2139

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 728.39 1012.96 1223.10 1068.94 889.58 894.25 1011.08 1024.22 817.49 919.89 1033.73 1049.45
Standard Deviation 91.10 171.52 159.79 161.42 121.22 134.56 144.17 181.01 135.12 151.45 159.95 171.67
Intensity Change Log 2 — 0.475794 0.747759 0.553398 — 0.007554 0.184701 0.203329 — 0.237279 0.465561 0.371498

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000159 0.000520 0.000941 0.000841 0.000193 0.000402 0.000554 0.001183 0.000178 0.000428 0.000595 0.000990
Bud Neck 0.002429 0.010439 0.009640 0.042580 0.003983 0.009268 0.015163 0.030492 0.003288 0.009521 0.014573 0.037313
Bud Site 0.000637 0.000929 0.000774 0.006929 0.000333 0.001729 0.001186 0.006795 0.000469 0.001557 0.001142 0.006871
Cell Periphery 0.000304 0.000109 0.000032 0.000139 0.000189 0.000219 0.000100 0.000645 0.000240 0.000195 0.000093 0.000359
Cytoplasm 0.004121 0.000760 0.000117 0.003873 0.000530 0.001018 0.001018 0.008800 0.002136 0.000962 0.000922 0.006020
Cytoplasmic Foci 0.000453 0.001180 0.000023 0.002197 0.000108 0.000590 0.000070 0.003870 0.000262 0.000717 0.000065 0.002926
Eisosomes 0.000019 0.000032 0.000052 0.000031 0.000036 0.000034 0.000042 0.000037 0.000028 0.000034 0.000043 0.000034
Endoplasmic Reticulum 0.005157 0.005893 0.002767 0.003586 0.005920 0.007943 0.003445 0.003860 0.005579 0.007500 0.003372 0.003705
Endosome 0.000262 0.000388 0.000048 0.004313 0.000262 0.000826 0.000486 0.006769 0.000262 0.000731 0.000439 0.005383
Golgi 0.000347 0.000535 0.000057 0.002826 0.000130 0.000341 0.000119 0.006513 0.000227 0.000383 0.000112 0.004432
Lipid Particles 0.000192 0.000062 0.000042 0.000130 0.000046 0.000556 0.000216 0.001301 0.000111 0.000449 0.000197 0.000641
Mitochondria 0.000591 0.001616 0.001259 0.004755 0.000368 0.002107 0.001312 0.005448 0.000468 0.002001 0.001306 0.005057
Mitotic Spindle 0.000543 0.001854 0.000040 0.022014 0.000861 0.003297 0.001736 0.035798 0.000719 0.002985 0.001554 0.028020
None 0.006586 0.003889 0.000556 0.002559 0.001746 0.001685 0.002483 0.007920 0.003910 0.002161 0.002277 0.004895
Nuclear Periphery 0.000415 0.000307 0.000120 0.002780 0.000263 0.000982 0.000366 0.002180 0.000331 0.000836 0.000339 0.002518
Nuclear Periphery Foci 0.000132 0.000106 0.000013 0.001080 0.000121 0.000813 0.000065 0.002099 0.000126 0.000660 0.000060 0.001524
Nucleolus 0.005278 0.007123 0.008089 0.006002 0.006434 0.006389 0.008315 0.006657 0.005917 0.006547 0.008291 0.006287
Nucleus 0.971110* 0.960874* 0.973748* 0.881720* 0.977781* 0.958683* 0.962193* 0.851469* 0.974798* 0.959156* 0.963427* 0.868539*
Peroxisomes 0.000146 0.000244 0.000700 0.000470 0.000117 0.000475 0.000279 0.000440 0.000130 0.000425 0.000324 0.000457
Vacuole 0.000972 0.001462 0.000893 0.007228 0.000454 0.002093 0.000638 0.011273 0.000686 0.001957 0.000666 0.008990
Vacuole Periphery 0.000149 0.001679 0.000089 0.003946 0.000124 0.000550 0.000217 0.006452 0.000136 0.000793 0.000203 0.005038

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.30 -11.51 -14.23 -8.46 -0.71 -5.41 -16.37 -15.46 -11.95 -9.98 -7.21 -17.12 -21.40 -14.71 -11.16
Bud Neck -7.26 -7.71 -16.14 -11.76 -12.19 -4.37 -12.09 -11.79 -8.89 -6.46 -6.56 -13.85 -19.85 -14.71 -11.91
Bud Site -0.65 -0.68 -7.04 -6.88 -7.06 -3.41 -7.85 -7.87 -5.32 -6.39 -3.07 -5.45 -10.63 -7.43 -8.99
Cell Periphery 2.86 4.86 3.39 -0.17 -8.24 -0.49 2.23 -1.01 -0.83 -1.55 0.93 4.41 0.18 -0.37 -1.75
Cytoplasm 3.69 4.55 0.70 -3.61 -4.78 -1.71 -2.41 -4.79 -4.33 -4.26 2.45 2.31 -3.51 -5.35 -5.28
Cytoplasmic Foci -0.62 2.18 -1.35 0.06 -2.81 -1.90 0.54 -3.19 -2.16 -3.30 -1.42 2.03 -3.44 -1.68 -4.27
Eisosomes -4.71 -9.56 -8.85 -1.46 5.15 0.42 -1.70 -2.08 -3.39 -0.72 -2.11 -6.30 -4.98 -2.88 2.08
Endoplasmic Reticulum -1.38 4.09 1.83 3.82 -3.51 -3.64 6.81 1.25 4.54 -5.16 -4.21 6.82 2.63 6.51 -5.15
Endosome -0.72 5.24 -3.75 -3.44 -4.19 -2.86 -0.76 -4.13 -3.35 -3.82 -3.10 -0.66 -5.57 -4.36 -4.95
Golgi -0.31 0.90 -1.70 -1.31 -2.47 -1.09 0.12 -2.64 -2.45 -2.67 -0.70 0.72 -3.22 -2.97 -3.52
Lipid Particles 1.41 1.56 0.51 -3.53 -5.21 -1.63 -0.95 -2.12 -0.46 -1.57 -1.36 -0.44 -1.97 0.11 -1.23
Mitochondria -1.81 -3.35 -7.86 -4.31 -6.97 -3.63 -5.53 -6.57 -2.98 -4.78 -3.80 -4.88 -10.45 -4.62 -7.74
Mitotic Spindle -0.95 1.02 -6.39 -5.52 -6.69 -2.09 -1.71 -7.01 -6.30 -6.68 -2.42 -1.98 -9.45 -8.09 -8.82
None 1.14 5.29 3.36 0.45 -8.56 0.09 -1.54 -5.05 -5.15 -4.45 2.21 2.56 -0.68 -3.20 -4.29
Nuclear Periphery 0.85 2.94 -5.92 -6.29 -6.86 -2.46 -1.92 -8.10 -3.09 -7.34 -2.21 -0.67 -9.02 -4.78 -8.73
Nuclear Periphery Foci 0.76 2.63 -3.41 -3.81 -4.51 -2.15 0.65 -3.85 -1.54 -3.97 -2.08 1.44 -5.04 -1.36 -5.42
Nucleolus -1.34 -2.84 -2.59 -0.74 0.18 -0.05 -2.33 -2.52 -2.35 -0.52 -1.11 -3.42 -3.40 -2.28 -0.24
Nucleus 1.90 -0.14 12.73 9.27 13.13 5.62 7.93 14.16 10.98 11.68 5.27 6.52 19.31 14.48 16.08
Peroxisomes -0.88 -5.98 -1.66 -0.92 3.25 -1.72 -1.51 -4.34 0.49 -0.97 -1.73 -2.03 -3.40 0.41 -0.32
Vacuole -0.86 -0.58 -5.35 -4.64 -5.23 -3.88 -2.53 -6.65 -5.48 -6.46 -3.57 -1.00 -8.60 -6.85 -8.47
Vacuole Periphery -1.29 1.64 -2.91 -0.30 -3.00 -3.34 -1.19 -3.82 -3.27 -3.72 -2.47 -0.98 -4.77 -3.13 -4.65
Standard name
Human Ortholog
Description Carboxy-terminal domain (CTD) phosphatase; essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p); relocalizes to the cytosol in response to hypoxia
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Fcp1

Fcp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fcp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available