Standard name
Human Ortholog
Description Homodimeric protein that is packaged into COPII vesicles; cycles between the ER and Golgi; involved in secretory transport but not directly required for aspects of transport assayed in vitro; may influence membrane composition
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.05 0.06 0.05 0.05 0.08 0 0 – 0.05 0 0.05 0 0 0 – 0.06 0.07 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0.07 0.06 0.06 0.05 0.08 0.07 0.06 0 – 0.05 0.09 0.06 0 0.12 0.18 – 0.44 0.46 0 0 0 0 0 0
Cytoplasm 0.13 0.16 0.13 0.21 0.22 0.21 0.15 0.17 – 0.15 0.23 0.18 0.19 0.17 0.21 – 0.38 0.29 0 0.07 0 0 0.06 0.05
Endoplasmic Reticulum 0.78 0.86 0.86 0.54 0.47 0.63 0.57 0.38 – 0.53 0.28 0.44 0.78 0.8 0.65 – 0 0 0.94 0.83 0.92 0.86 0.81 0.83
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0.07 0 0 0 0 0 0
Mitochondria 0 0 0.07 0.19 0.29 0.22 0.3 0.2 – 0.23 0.37 0.37 0 0 0 – 0.19 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0.05 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.17 0 0 0.15 0.15 0 0.12 0.33 – 0.19 0.18 0.12 0.05 0 0.1 – 0 0.12 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 6 13 9 15 25 40 26 30 – 31 9 32 12 22 13 – 1 3 2 5 0 2 7 9
Bud 1 2 4 5 16 16 19 17 – 15 22 16 1 1 1 – 0 0 0 3 0 1 3 2
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 1 0 0 3 1
Bud Site 0 0 0 0 0 1 0 0 – 0 2 1 0 0 0 – 0 0 – – – – – –
Cell Periphery 15 14 9 18 37 37 39 22 – 31 41 40 15 65 100 – 7 19 1 3 0 1 1 7
Cytoplasm 26 40 19 70 102 103 94 120 – 85 102 118 70 89 117 – 6 12 4 18 4 8 22 20
Endoplasmic Reticulum 158 211 125 178 212 312 361 270 – 301 128 289 293 426 371 – 0 0 197 227 153 191 315 365
Endosome 1 0 0 5 12 1 2 8 – 3 3 1 0 1 2 – 0 0 0 0 1 2 1 1
Golgi 2 0 0 4 1 0 0 0 – 0 0 0 1 3 1 – 0 3 0 0 0 0 0 3
Mitochondria 0 0 10 64 130 107 191 142 – 132 167 243 1 1 0 – 3 0 0 2 4 5 13 10
Nucleus 1 0 0 1 0 1 2 7 – 6 4 6 1 0 3 – 0 0 0 0 0 0 0 1
Nuclear Periphery 1 0 2 5 7 7 17 18 – 26 14 28 3 1 1 – 0 0 0 1 0 2 2 1
Nucleolus 0 0 0 0 0 0 0 3 – 0 1 1 0 0 1 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 34 6 3 48 69 20 76 239 – 110 83 77 19 12 55 – 0 5 0 6 0 5 15 15
Unique Cell Count 202 246 146 330 455 496 633 720 571 450 653 376 531 567 16 41 211 273 167 223 391 443
Labelled Cell Count 245 286 181 413 612 645 827 876 740 576 852 416 621 665 17 42 211 273 167 223 391 443


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.2 12.2 8.3 8.0 7.6 7.8 7.2 7.7 – 7.8 7.6 7.6 10.6 10.9 10.7 – 16.4 18.0 11.4 11.3 10.8
Std Deviation (1e-4) 1.8 1.9 1.3 1.3 1.4 1.3 1.3 1.3 – 1.4 1.3 1.4 1.6 1.5 1.6 – 3.0 4.1 1.7 1.9 1.6
Intensity Change (Log2) – – – -0.05 -0.12 -0.1 -0.2 -0.1 – -0.08 -0.13 -0.12 0.35 0.39 0.37 – 0.99 1.12 0.46 0.45 0.38


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.7 -0.3 0.8 -1.1 -1.1 – -0.3 0 -0.6 -1.6 -1.0 0 – 0 0
Bud 0 0 0 0 0 – 0 1.1 0 0 0 0 – 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Cell Periphery -0.3 0.8 0.5 0 -1.8 – -0.3 1.1 0 -1.1 2.1 3.4 – 0 6.4
Cytoplasm 2.1 2.5 2.1 0.6 1.1 – 0.6 2.5 1.5 1.5 1.1 2.1 – 0 2.5
Endoplasmic Reticulum -6.6 -8.3 -5.2 -6.4 -10.6 – -7.2 -12.1 -9.0 -2.0 -1.5 -4.7 – -7.7 -10.3
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Mitochondria 3.5 5.4 4.1 5.8 3.7 – 4.4 7.0 7.1 0 0 0 – 0 0
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Nuclear Periphery 0 0 0 0 0 – 1.8 0 1.7 0 0 0 – 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Vacuole 4.1 4.2 1.1 3.6 7.6 – 5.1 4.9 3.5 1.5 0 3.0 – 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.1668 6.5561 6.0064 5.4222 5.8933 6.248 5.8255 6.9582 6.2315 5.9416 6.6809 6.4599 5.2924 5.6096 5.5821 5.3577 5.842 5.28
Actin 0.0498 0.0056 0.0361 0.0006 0.0028 0.0022 0.0358 0.0011 0.0131 0.0004 0.0004 0.0159 0.0003 0.0019 0.0011 0.0005 0.0002 0.0007
Bud 0.0008 0.0002 0.0006 0.0001 0.0001 0.0037 0.0002 0.0001 0.0002 0 0 0.0002 0 0.0001 0 0 0 0
Bud Neck 0.0016 0.0006 0.0035 0.0005 0.0003 0.0016 0.0015 0.0004 0.0058 0.0002 0.0003 0.0019 0.0001 0.0004 0.0012 0.0001 0.0002 0.0014
Bud Periphery 0.0013 0.0002 0.001 0.0002 0.0002 0.0035 0.0003 0.0001 0.0003 0 0 0.0004 0 0.0001 0.0002 0 0 0.0001
Bud Site 0.0034 0.0042 0.0029 0.0001 0.0001 0.0002 0.0053 0.0008 0.0021 0 0 0.0002 0.0001 0.0024 0.0002 0 0 0
Cell Periphery 0.002 0.0045 0.0008 0.001 0.0003 0.0007 0.0024 0.0037 0.0016 0.0005 0.0008 0.0018 0.0012 0.0042 0.001 0.0005 0.0007 0.0006
Cytoplasm 0.104 0.1718 0.0848 0.1157 0.1609 0.1681 0.0425 0.2086 0.0777 0.185 0.1014 0.1002 0.0449 0.1235 0.0644 0.0507 0.0582 0.0774
Cytoplasmic Foci 0.0075 0.0059 0.0093 0.0061 0.0009 0.0059 0.0352 0.0041 0.0212 0.0009 0.0014 0.0043 0.0008 0.0029 0.0018 0.0007 0.0009 0.0013
Eisosomes 0.0008 0.0001 0.0003 0 0 0 0.0004 0 0.0001 0 0 0.0001 0 0.0002 0 0 0 0
Endoplasmic Reticulum 0.7039 0.7645 0.7501 0.7882 0.7663 0.7817 0.744 0.7418 0.7849 0.8026 0.8647 0.8188 0.9339 0.8194 0.9116 0.9327 0.9224 0.9017
Endosome 0.0337 0.0097 0.0289 0.012 0.0178 0.0092 0.029 0.0092 0.0351 0.0022 0.013 0.0158 0.0029 0.0054 0.0054 0.0032 0.0066 0.0057
Golgi 0.0079 0.0047 0.0149 0.0009 0.0026 0.002 0.0306 0.0021 0.0172 0.0004 0.001 0.0063 0.0006 0.0018 0.002 0.0004 0.0006 0.0018
Lipid Particles 0.0158 0.0054 0.0202 0.0069 0.0022 0.0017 0.0262 0.0109 0.0118 0.0017 0.0019 0.0145 0.0045 0.0049 0.0037 0.0008 0.0019 0.0009
Mitochondria 0.0077 0.0042 0.0161 0.0002 0.006 0.0006 0.0041 0.0003 0.0034 0.0001 0.0002 0.004 0.0001 0.0003 0.0001 0.0001 0.0001 0.0023
None 0.0151 0.002 0.002 0.0554 0.0008 0.009 0.003 0.0032 0.0065 0.0011 0.001 0.0016 0.001 0.0031 0.0005 0.0004 0.0004 0.0006
Nuclear Periphery 0.023 0.0085 0.0132 0.0067 0.0307 0.0049 0.0166 0.0074 0.0068 0.003 0.0066 0.0074 0.0075 0.0214 0.0041 0.0074 0.0048 0.0034
Nucleolus 0.0005 0.0001 0.0003 0 0.0001 0 0.0002 0 0.0001 0 0 0 0 0 0 0 0 0
Nucleus 0.0063 0.0017 0.0022 0.0016 0.0023 0.0019 0.0057 0.001 0.0006 0.0004 0.0007 0.0012 0.0005 0.0032 0.0004 0.0004 0.0007 0.0005
Peroxisomes 0.0053 0.0016 0.0065 0 0.0004 0.0002 0.0088 0 0.0067 0 0 0.0009 0 0.0001 0 0 0 0
Punctate Nuclear 0.0026 0.0004 0.0011 0.0003 0.0006 0.0003 0.0043 0.0003 0.0007 0.0001 0.0001 0.0003 0.0001 0.0003 0.0001 0.0001 0.0001 0.0001
Vacuole 0.005 0.0038 0.0037 0.0034 0.0023 0.0024 0.0032 0.0045 0.0034 0.0013 0.0061 0.0034 0.0013 0.0037 0.0019 0.0014 0.002 0.0013
Vacuole Periphery 0.0018 0.0003 0.0016 0.0003 0.0022 0.0003 0.0007 0.0003 0.0007 0.0002 0.0005 0.0007 0.0002 0.0006 0.0002 0.0003 0.0002 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 87.3198 66.2315 31.5943 60.6198 106.3525 60.8255 65.1728 64.5685 51.8202 104.1013
Translational Efficiency 1.0405 1.0216 1.3883 0.6358 0.723 1.7141 1.001 0.8617 0.9766 0.9167

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
359 347 3001 1449 1378 1387 2684 2042 1737 1734 5685 3491

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 804.97 1033.88 1356.30 1363.46 789.17 1076.24 1419.36 1417.12 792.44 1067.76 1386.07 1394.85
Standard Deviation 126.49 187.55 179.82 225.68 147.17 165.34 203.80 213.37 143.28 170.86 194.09 220.16
Intensity Change Log 2 — 0.361062 0.752669 0.760265 — 0.447592 0.846833 0.844554 — 0.404547 0.800053 0.802608

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001436 0.002866 0.001432 0.004433 0.001328 0.003630 0.001406 0.003462 0.001350 0.003477 0.001419 0.003865
Bud Neck 0.001902 0.002167 0.001714 0.006988 0.002994 0.001875 0.001547 0.002342 0.002768 0.001933 0.001635 0.004270
Bud Site 0.015448 0.020420 0.008588 0.041256 0.014789 0.013885 0.005879 0.017567 0.014925 0.015193 0.007309 0.027400
Cell Periphery 0.004503 0.003490 0.004125 0.012621 0.001195 0.002616 0.008610 0.012778 0.001879 0.002791 0.006242 0.012713
Cytoplasm 0.038093 0.082652 0.075544 0.082193 0.137292* 0.099647 0.066258 0.070253 0.116789* 0.096246 0.071160 0.075209
Cytoplasmic Foci 0.021851 0.048449 0.003098 0.017634 0.102412 0.031492 0.001090 0.006883 0.085762 0.034886 0.002150 0.011345
Eisosomes 0.000459 0.000329 0.000152 0.000391 0.000219 0.000795 0.000284 0.000341 0.000268 0.000702 0.000214 0.000362
Endoplasmic Reticulum 0.855496* 0.732629* 0.862130* 0.676330* 0.547435* 0.769555* 0.887784* 0.817947* 0.611104* 0.762165* 0.874242* 0.759166*
Endosome 0.005574 0.019401 0.004943 0.016761 0.025631 0.009459 0.002270 0.007723 0.021486 0.011449 0.003681 0.011474
Golgi 0.005365 0.016930 0.001708 0.006240 0.033357 0.016934 0.000790 0.003857 0.027572 0.016933 0.001275 0.004846
Lipid Particles 0.008151 0.018977 0.008426 0.037184 0.043555 0.019467 0.006166 0.026130 0.036237 0.019369 0.007359 0.030718
Mitochondria 0.013704 0.005196 0.001668 0.001044 0.008887 0.003282 0.001633 0.001453 0.009882 0.003665 0.001651 0.001284
Mitotic Spindle 0.000133 0.000210 0.000345 0.008974 0.000531 0.001608 0.000041 0.000847 0.000449 0.001328 0.000201 0.004220
None 0.001347 0.002770 0.003010 0.000725 0.009354 0.001403 0.004663 0.001583 0.007699 0.001677 0.003791 0.001227
Nuclear Periphery 0.001850 0.001016 0.001082 0.003365 0.007318 0.000587 0.000184 0.000259 0.006188 0.000673 0.000658 0.001548
Nuclear Periphery Foci 0.004925 0.010737 0.006668 0.028514 0.020033 0.005156 0.001814 0.004808 0.016910 0.006273 0.004376 0.014647
Nucleolus 0.000201 0.000147 0.000041 0.000194 0.001265 0.000059 0.000031 0.000046 0.001045 0.000077 0.000037 0.000107
Nucleus 0.007313 0.002797 0.004008 0.006137 0.009073 0.003690 0.002016 0.002489 0.008709 0.003512 0.003067 0.004003
Peroxisomes 0.004609 0.001746 0.000350 0.001044 0.002479 0.002231 0.000906 0.001611 0.002920 0.002134 0.000612 0.001376
Vacuole 0.003709 0.019984 0.010411 0.045621 0.022351 0.009832 0.006388 0.015696 0.018499 0.011864 0.008511 0.028117
Vacuole Periphery 0.003931 0.007084 0.000557 0.002350 0.008502 0.002797 0.000241 0.001926 0.007557 0.003655 0.000408 0.002102

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.32 -0.24 -4.18 -1.60 -4.66 -3.50 -0.46 -3.25 0.11 -2.95 -3.74 -0.65 -5.34 -0.79 -5.12
Bud Neck -0.19 0.03 -4.23 -6.29 -8.08 1.65 1.93 0.81 -1.61 -1.95 1.38 1.72 -2.99 -6.87 -7.62
Bud Site -0.94 1.56 -5.38 -4.60 -9.58 0.38 5.02 -1.68 -1.95 -6.40 -0.15 4.55 -6.16 -5.88 -11.22
Cell Periphery 0.69 0.28 -4.49 -5.71 -6.15 -4.80 -11.30 -12.69 -10.76 -3.70 -2.39 -9.80 -13.51 -12.29 -7.57
Cytoplasm -5.03 -7.55 -7.97 -0.64 -2.33 5.81 12.98 11.39 5.39 -1.15 3.71 10.11 7.95 3.97 -2.05
Cytoplasmic Foci -3.98 4.99 0.80 5.22 -9.69 14.88 24.32 22.52 10.08 -7.42 12.44 23.97 20.68 10.03 -11.72
Eisosomes 0.65 1.70 0.38 -0.53 -3.58 -5.79 -1.40 -3.07 4.38 -0.90 -4.83 1.07 -1.77 3.93 -3.52
Endoplasmic Reticulum 5.44 -0.19 11.85 3.93 18.86 -16.77 -31.01 -22.26 -4.33 10.14 -12.73 -27.20 -12.64 1.59 19.68
Endosome -5.64 0.50 -9.54 0.86 -11.72 11.13 18.46 13.30 1.94 -11.02 7.86 17.01 8.56 -0.27 -14.94
Golgi -4.19 4.16 -0.60 3.88 -4.64 6.65 16.19 14.40 8.82 -7.73 5.17 16.12 13.49 9.09 -7.64
Lipid Particles -3.15 -0.32 -8.65 -5.04 -10.49 7.85 13.83 5.39 -2.75 -10.19 6.59 12.92 1.94 -5.63 -14.38
Mitochondria 2.46 3.97 4.17 2.48 2.97 6.65 9.37 9.62 4.29 0.62 6.37 9.41 9.84 5.05 2.07
Mitotic Spindle -1.53 -2.61 -5.85 -5.80 -5.69 -1.36 6.10 -0.74 0.82 -1.82 -1.38 2.63 -5.61 -3.46 -5.88
None -1.03 -2.44 1.38 1.58 4.51 5.15 2.89 5.06 -0.33 4.16 4.79 3.14 5.35 0.98 5.62
Nuclear Periphery 0.76 0.71 -1.42 -3.33 -3.62 5.25 5.58 5.51 3.26 -3.94 5.28 5.30 4.23 -3.25 -3.35
Nuclear Periphery Foci -1.94 -1.35 -8.62 -5.25 -8.85 8.26 11.07 8.58 0.19 -4.49 6.79 8.88 0.94 -6.49 -9.06
Nucleolus 0.50 1.81 -0.17 -0.75 -2.30 3.31 3.38 3.34 1.03 -2.24 3.33 3.48 3.17 -1.18 -2.52
Nucleus 1.50 1.07 0.11 -5.63 -4.25 6.21 8.26 6.85 1.64 -1.69 5.47 5.82 4.06 -2.81 -3.63
Peroxisomes 1.88 2.85 2.37 2.00 -4.89 0.74 4.91 2.75 2.14 -2.63 1.89 6.05 3.95 3.31 -4.97
Vacuole -6.00 -6.11 -14.48 -8.10 -12.56 6.19 8.59 2.62 -4.08 -7.10 3.84 6.26 -6.27 -10.87 -13.78
Vacuole Periphery -1.62 4.18 1.77 2.61 -3.96 6.76 13.30 8.99 1.25 -4.41 5.00 13.65 9.17 2.41 -5.77
Standard name
Human Ortholog
Description Homodimeric protein that is packaged into COPII vesicles; cycles between the ER and Golgi; involved in secretory transport but not directly required for aspects of transport assayed in vitro; may influence membrane composition
Localization
Cell Percentages ER (98%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Got1

Got1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Got1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available