ORF
Human Ortholog
Description Protein of unknown function that associates with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and bud; not an essential gene; protein abundance increases in response to DNA replication stress; YMR295C has a paralog, YGR273C, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.23 0.25 0.27 0.46 0.24 0.28 0.26 – 0.28 0.24 0.28 0.22 0.34 0.28 0.25 0.14 0.08 0.11 0.12 0.11 0.13 0.12 0.15 0.19
Bud 0.08 0.17 0.16 0.21 0.19 0.18 0.15 – 0.17 0.12 0.15 0.14 0.13 0.11 0.14 0.06 0 0.06 0.31 0.44 0.33 0.35 0.32 0.31
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.09 0.12 0.08 0.17 0.11 0.08
Bud Site 0 0.08 0.07 0.08 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.28 0.25 0.3 0.36 0.14 0.2 0.34 – 0.29 0.24 0.33 0.29 0.45 0.52 0.62 0.17 0.17 0.17 0.21 0.14 0.21 0.1 0.18 0.12
Cytoplasm 0.09 0.08 0.09 0.07 0.17 0.2 0.23 – 0.27 0.26 0.31 0.34 0.07 0.08 0.07 0.14 0.14 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0.14 0.07 0 0.07 0.1 0.11 0.1 – 0.06 0.06 0.1 0.14 0.13 0.1 0.1 0 0.05 0 0.06 0 0 0 0.05 0.11
Endosome 0.09 0.08 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0.09 0 0 – 0 0 0 0 0.06 0 0 0.13 0.18 0.21 0 0 0 0 0 0
Mitochondria 0 0.05 0 0 0 0.06 0 – 0 0 0 0 0 0 0 0.18 0 0.06 0.05 0 0.08 0.15 0.11 0.09
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.11 0.16 0.1 0.07 0.07 0.13 0.07 – 0.1 0.11 0.07 0 0 0 0 0.11 0.14 0.13 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.05 0.05 0.08 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.17 0.1 0.14 0.06 0.22 0.16 0.17 – 0.16 0.24 0.15 0.13 0.05 0 0 0.28 0.36 0.24 0.06 0.05 0.08 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 39 28 50 108 55 61 107 – 143 112 103 93 62 79 56 20 9 16 29 18 33 14 39 52
Bud 13 19 29 49 44 39 60 – 87 56 53 60 24 30 31 8 4 9 73 72 86 44 88 85
Bud Neck 2 0 2 1 0 1 0 – 0 0 1 1 0 0 0 1 0 1 21 20 22 21 29 23
Bud Site 5 9 12 19 0 8 2 – 5 4 4 4 5 3 2 2 1 1 – – – – – –
Cell Periphery 47 28 55 86 32 42 138 – 153 113 121 123 81 148 140 25 20 25 50 22 54 12 50 32
Cytoplasm 15 9 17 16 38 43 93 – 141 122 111 142 12 23 16 20 17 16 2 3 0 0 3 4
Endoplasmic Reticulum 23 8 7 16 23 24 43 – 32 26 36 59 23 29 23 4 6 5 14 2 6 2 13 32
Endosome 15 9 7 7 1 1 10 – 3 3 0 4 6 11 1 4 4 6 2 1 1 0 2 3
Golgi 3 0 3 7 21 1 10 – 9 17 7 4 11 11 5 19 21 31 3 0 1 1 0 3
Mitochondria 6 6 1 10 0 12 1 – 6 6 4 12 6 7 5 26 2 9 11 4 22 19 29 25
Nucleus 1 2 1 2 0 5 2 – 6 4 1 1 2 1 1 0 0 1 0 1 1 0 0 0
Nuclear Periphery 0 0 0 0 0 1 0 – 2 2 2 3 0 2 2 0 0 0 4 3 3 0 3 5
Nucleolus 19 18 19 16 17 27 28 – 54 49 24 18 2 3 0 16 16 19 1 0 2 0 1 0
Peroxisomes 1 0 0 2 0 1 0 – 0 0 0 0 6 11 8 1 0 0 0 0 0 0 0 0
SpindlePole 8 6 15 6 10 1 8 – 1 4 3 2 2 6 2 2 1 3 0 1 4 0 0 0
Vac/Vac Membrane 29 11 25 15 51 35 70 – 84 110 55 54 9 9 3 40 43 36 15 7 20 3 6 6
Unique Cell Count 168 114 183 236 227 215 410 519 462 362 420 181 283 226 145 118 149 236 165 266 125 275 281
Labelled Cell Count 226 153 243 360 292 302 572 726 628 525 580 251 373 295 188 144 178 236 165 266 125 275 281


Bud, Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 24.4 22.0 22.1 20.0 23.6 16.7 22.2 – 20.0 20.6 19.6 19.1 24.8 24.5 25.3 38.9 36.7 40.1 23.5 22.9 22.7
Std Deviation (1e-4) 15.9 13.5 11.0 7.0 10.4 6.7 7.7 – 6.4 6.1 6.7 6.2 12.9 12.5 11.5 24.5 20.8 25.1 11.8 8.9 8.3
Intensity Change (Log2) – – – -0.14 0.09 -0.41 0.01 – -0.14 -0.1 -0.17 -0.21 0.17 0.15 0.2 0.82 0.73 0.86 0.09 0.06 0.04

WT3RAP60RAP140RAP220RAP300RAP460RAP540RAP620RAP7000204060WT3HU80HU120HU1600204060WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30204060WT1AF100AF140AF1800204060
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 3.9 -0.7 0.2 -0.3 – 0.1 -0.8 0.3 -1.4 1.4 0.1 -0.6 -3.0 -4.2 -3.8
Bud 1.3 0.9 0.6 -0.4 – 0.3 -1.3 -0.4 -0.5 -0.7 -1.7 -0.6 -2.9 -3.4 -2.8
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0.6 -3.9 -1.3 0 – 0 0 -3.6 0 -1.7 -3.3 -3.1 -2.3 -2.4 -2.8
Cell Periphery 1.4 -3.9 -2.4 0.9 – -0.1 -1.5 0.8 -0.2 2.9 4.7 6.4 -2.7 -2.6 -2.8
Cytoplasm -0.9 2.2 3.0 3.9 – 5.0 4.8 5.6 6.3 -0.9 -0.4 -0.8 1.3 1.4 0.4
Endoplasmic Reticulum 1.3 2.4 2.7 2.7 – 1.2 0.9 2.5 3.7 3.1 2.5 2.4 0 0.5 -0.2
Endosome -0.5 0 0 -0.9 – 0 0 0 0 -0.3 0 0 0 0 0.1
Golgi 0 3.3 0 0 – 0 1.3 0 0 2.2 1.4 0 4.1 5.1 5.7
Mitochondria 0 0 2.8 0 – 0 0 0 0 0 0 0 5.7 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus -1.3 -1.0 0.7 -1.5 – 0 0.1 -1.5 -2.9 -3.8 -4.6 -5.0 0.2 0.8 0.7
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole -2.6 -1.6 -3.9 -3.6 – 0 -5.0 -4.5 -5.3 -3.2 -3.1 -3.7 -2.8 -2.8 -2.5
Vacuole -2.5 2.3 0.7 1.0 – 0.8 2.9 0.5 -0.3 -2.8 -4.2 -4.9 3.1 4.6 2.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 19.1172 14.7259 14.7893 14.2287 14.1064 16.5151 24.0263 14.7793 18.22 18.2023 17.0227 20.6477 24.9129 18.4723 20.3412 24.499 22.3408 22.9569
Actin 0.0587 0.0667 0.0506 0.0809 0.116 0.0473 0.0514 0.0525 0.0291 0.1246 0.009 0.0868 0.0515 0.0356 0.0468 0.0157 0.0145 0.0872
Bud 0.0244 0.0354 0.2556 0.1139 0.0762 0.0162 0.0087 0.0702 0.2527 0.1777 0.0719 0.0139 0.0167 0.0712 0.2824 0.118 0.0977 0.0154
Bud Neck 0.0342 0.0114 0.0116 0.0162 0.1613 0.4996 0.0288 0.0092 0.0012 0.0015 0.0033 0.5689 0.0125 0.0098 0.0088 0.0048 0.1383 0.4579
Bud Periphery 0.0658 0.0625 0.3986 0.5118 0.2882 0.1239 0.0448 0.1131 0.4886 0.4211 0.5782 0.1011 0.0819 0.1259 0.4255 0.4723 0.3676 0.1191
Bud Site 0.197 0.4641 0.0892 0.0222 0.0076 0.0103 0.1037 0.3599 0.0465 0.0206 0.0048 0.0113 0.1311 0.4203 0.0842 0.0179 0.1559 0.0084
Cell Periphery 0.4374 0.2616 0.0599 0.148 0.0923 0.179 0.5338 0.1402 0.0601 0.0611 0.2845 0.0993 0.475 0.1964 0.0755 0.1497 0.0549 0.1804
Cytoplasm 0.0215 0.0181 0.0086 0.005 0.0054 0.0062 0.0482 0.0232 0.0027 0.0062 0.0013 0.0095 0.0201 0.0319 0.0036 0.0031 0.0018 0.0067
Cytoplasmic Foci 0.0163 0.0067 0.0094 0.0012 0.0129 0.0064 0.0173 0.0361 0.0021 0.0057 0 0.0063 0.0225 0.0081 0.0027 0.0004 0.0019 0.0086
Eisosomes 0.0149 0.0034 0.0004 0.0008 0.0037 0.0016 0.0068 0.0017 0.0002 0.0004 0.0006 0.0042 0.0089 0.0007 0.0012 0.0006 0.0012 0.0038
Endoplasmic Reticulum 0.0134 0.0028 0.0046 0.0012 0.0081 0.0038 0.0217 0.0041 0.0024 0.0038 0.0013 0.0077 0.0241 0.0028 0.0028 0.0016 0.0022 0.0033
Endosome 0.0263 0.008 0.0203 0.0027 0.0756 0.0152 0.0096 0.025 0.0152 0.0182 0.0002 0.0055 0.0301 0.0183 0.0023 0.0009 0.0688 0.0163
Golgi 0.0064 0.0033 0.0042 0.0062 0.0353 0.03 0.0069 0.0133 0.0108 0.0233 0.0001 0.0074 0.012 0.0046 0.0054 0.0002 0.0082 0.0181
Lipid Particles 0.0109 0.0053 0.0082 0.0023 0.034 0.0127 0.0134 0.0133 0.0013 0.0074 0.0003 0.0133 0.0251 0.0067 0.0031 0.0027 0.0023 0.0112
Mitochondria 0.0101 0.003 0.0154 0.0176 0.0395 0.0028 0.0035 0.0039 0.0184 0.061 0.0022 0.0062 0.005 0.0024 0.0058 0.0682 0.015 0.003
None 0.0054 0.0005 0.0013 0.0002 0.0005 0.0001 0.0053 0.0008 0.0002 0.0008 0.0002 0.0177 0.0023 0.0004 0.0006 0.0002 0.0003 0.0001
Nuclear Periphery 0.005 0.0015 0.0052 0.0009 0.0032 0.0009 0.0079 0.0016 0.013 0.0031 0.0007 0.0007 0.0081 0.0014 0.0018 0.001 0.0039 0.0008
Nucleolus 0.0034 0.0025 0.0034 0.0197 0.0015 0.0018 0.0142 0.0068 0.0011 0.001 0.001 0.0031 0.0023 0.0132 0.0072 0.0017 0.0014 0.0014
Nucleus 0.0026 0.0018 0.0064 0.0064 0.0023 0.0023 0.0041 0.0057 0.0023 0.0023 0.0015 0.0019 0.0021 0.0031 0.0022 0.0028 0.0026 0.0019
Peroxisomes 0.0035 0.0008 0.0041 0.0018 0.0024 0.0019 0.0044 0.0011 0.0009 0.0071 0.0001 0.0054 0.0092 0.0009 0.0015 0.0025 0.0004 0.0065
Punctate Nuclear 0.0016 0.0002 0.0052 0.0002 0.0006 0.0002 0.0041 0.001 0.0001 0.0004 0 0.0005 0.0022 0.0003 0.0002 0 0.0002 0.0003
Vacuole 0.0385 0.0381 0.0329 0.0369 0.0242 0.034 0.0588 0.109 0.0412 0.0452 0.0338 0.0258 0.051 0.0404 0.0324 0.1192 0.0449 0.0442
Vacuole Periphery 0.0025 0.0022 0.0046 0.004 0.0093 0.0037 0.0026 0.0083 0.01 0.0074 0.0049 0.0034 0.0062 0.0056 0.0042 0.0165 0.0161 0.0055

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 178.328 192.6256 103.5058 71.5947 186.6539 135.5285 221.2078 136.5517 134.868 131.1511
Translational Efficiency 2.1524 2.3572 1.7682 2.6679 1.8665 3.0799 1.7815 2.3402 1.6527 1.9972

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
684 1108 2233 563 1792 2082 1872 1583 2476 3190 4105 2146

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1338.82 1734.50 2086.28 2158.76 1573.82 1765.88 1942.21 2502.06 1508.90 1754.98 2020.58 2412.00
Standard Deviation 344.10 552.41 500.14 597.82 390.16 490.57 513.96 710.47 392.33 513.11 511.55 699.22
Intensity Change Log 2 — 0.373558 0.639971 0.689241 — 0.166117 0.303429 0.668846 — 0.265184 0.467912 0.678256

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.017716 0.028456 0.028156 0.019329 0.013638 0.018773 0.018715 0.009633 0.014765 0.022136 0.023850 0.012177
Bud Neck 0.069186 0.069168 0.102643 0.059783 0.081259 0.077406 0.087233 0.048067 0.077924 0.074544 0.095616 0.051141
Bud Site 0.494122 0.422081 0.359931 0.476663 0.296710 0.420203 0.505259 0.260149 0.351246 0.420856 0.426205 0.316951
Cell Periphery 0.265274 0.328078 0.453069 0.325403 0.547429 0.411569 0.325892 0.610325 0.469484 0.382570 0.395072 0.535576
Cytoplasm 0.054917 0.028900 0.004061 0.016532 0.008017 0.011365 0.011719 0.009882 0.020973 0.017455 0.007554 0.011627
Cytoplasmic Foci 0.010101 0.008981 0.000990 0.004613 0.002565 0.003790 0.001599 0.000993 0.004647 0.005593 0.001268 0.001943
Eisosomes 0.001647 0.004180 0.006750 0.005004 0.004509 0.003335 0.004335 0.004479 0.003718 0.003629 0.005649 0.004617
Endoplasmic Reticulum 0.011689 0.014675 0.009221 0.007236 0.015430 0.010295 0.005607 0.003882 0.014396 0.011816 0.007573 0.004762
Endosome 0.012090 0.012151 0.003939 0.009956 0.005288 0.007319 0.003565 0.002961 0.007167 0.008997 0.003768 0.004796
Golgi 0.016439 0.016159 0.002093 0.006644 0.003072 0.004853 0.002295 0.003479 0.006765 0.008780 0.002185 0.004310
Lipid Particles 0.007571 0.014648 0.003526 0.028944 0.002299 0.001970 0.007089 0.013843 0.003755 0.006374 0.005151 0.017805
Mitochondria 0.002064 0.003960 0.004492 0.001573 0.003969 0.007649 0.003302 0.002169 0.003442 0.006368 0.003949 0.002012
Mitotic Spindle 0.001386 0.000479 0.000497 0.000542 0.000347 0.000612 0.000408 0.000371 0.000634 0.000566 0.000457 0.000416
None 0.000392 0.000836 0.000111 0.000092 0.000160 0.000397 0.000116 0.000090 0.000224 0.000550 0.000113 0.000090
Nuclear Periphery 0.000088 0.000178 0.000167 0.000395 0.000210 0.000105 0.000110 0.000290 0.000176 0.000131 0.000141 0.000318
Nuclear Periphery Foci 0.000686 0.000809 0.000171 0.000558 0.000211 0.000196 0.000229 0.000227 0.000342 0.000409 0.000197 0.000314
Nucleolus 0.000119 0.000188 0.000113 0.000104 0.000109 0.000119 0.000105 0.000076 0.000112 0.000143 0.000109 0.000083
Nucleus 0.002659 0.002300 0.000688 0.001102 0.000592 0.000802 0.001092 0.000881 0.001163 0.001322 0.000872 0.000939
Peroxisomes 0.001288 0.000849 0.000121 0.000327 0.000399 0.000338 0.000187 0.000285 0.000644 0.000515 0.000151 0.000296
Vacuole 0.025266 0.035069 0.016396 0.030161 0.010849 0.013912 0.018268 0.025653 0.014832 0.021261 0.017250 0.026836
Vacuole Periphery 0.005301 0.007856 0.002866 0.005042 0.002936 0.004992 0.002874 0.002262 0.003590 0.005986 0.002870 0.002992

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.84 -3.35 -0.40 2.25 2.57 -2.71 -2.47 2.10 5.17 4.63 -4.15 -5.26 1.48 5.70 6.86
Bud Neck 0.03 -5.12 1.35 1.55 7.17 0.77 -1.06 6.87 7.03 9.04 0.85 -4.23 6.67 6.85 12.96
Bud Site 4.42 9.15 0.78 -3.13 -7.12 -11.56 -19.28 3.25 14.59 22.11 -7.69 -8.85 3.32 10.84 12.09
Cell Periphery -3.91 -12.92 -3.05 0.22 7.79 11.34 18.89 -4.76 -15.96 -23.31 8.77 8.01 -5.68 -14.46 -13.95
Cytoplasm 4.77 10.66 7.24 3.53 -5.43 -2.64 -3.30 -1.41 0.98 1.60 1.75 8.03 5.04 3.80 -3.16
Cytoplasmic Foci 0.47 5.79 2.61 2.41 -2.57 -1.53 1.08 2.54 4.25 2.27 -1.26 5.14 3.71 5.18 -1.22
Eisosomes -3.50 -9.56 -4.50 -0.83 2.15 2.11 0.29 0.05 -2.58 -0.28 0.21 -3.81 -1.77 -2.27 2.18
Endoplasmic Reticulum -1.39 1.38 2.01 3.43 1.13 3.20 6.20 7.56 6.98 2.02 1.96 5.45 7.65 7.72 3.54
Endosome -0.07 5.91 0.84 0.93 -2.68 -2.32 2.23 2.99 5.33 1.01 -2.33 5.27 2.66 4.78 -1.33
Golgi 0.09 5.44 3.08 3.45 -2.39 -1.89 1.15 -0.39 1.14 -1.16 -1.67 5.29 2.12 3.68 -2.40
Lipid Particles -2.79 2.39 -3.78 -2.39 -4.63 0.54 -3.45 -5.22 -5.39 -2.65 -2.82 -1.68 -6.35 -4.96 -5.58
Mitochondria -1.92 -5.08 1.16 2.50 7.13 -4.31 1.20 3.36 6.79 2.28 -4.40 -1.24 3.51 6.90 5.55
Mitotic Spindle 1.09 1.02 1.00 -0.46 -0.11 -2.69 -0.99 -0.44 2.38 0.69 0.27 0.61 0.93 1.96 0.55
None -2.07 2.19 2.33 4.48 1.26 -1.19 2.16 3.87 1.53 1.92 -2.21 2.87 3.47 3.23 2.33
Nuclear Periphery -4.66 -5.36 -4.21 -2.89 -3.08 3.69 3.18 -2.21 -7.02 -6.17 2.11 1.54 -4.31 -6.75 -6.25
Nuclear Periphery Foci -0.99 7.75 0.57 1.08 -1.79 0.64 -0.84 -0.50 -0.90 0.17 -1.46 5.73 0.45 1.35 -1.83
Nucleolus -2.80 0.33 0.66 3.30 0.51 -0.74 0.21 3.26 2.69 2.55 -2.32 0.16 2.97 4.45 3.10
Nucleus 0.20 1.36 1.05 2.04 -3.06 -1.61 -4.68 -2.71 -0.32 2.13 -0.46 0.44 0.48 1.67 0.13
Peroxisomes 1.66 5.14 4.00 3.29 -2.50 0.83 3.57 1.09 0.53 -1.02 1.37 6.54 3.43 2.43 -2.00
Vacuole -2.96 3.28 -1.40 1.12 -4.20 -2.60 -5.70 -8.39 -6.09 -3.14 -4.83 -2.57 -7.22 -3.08 -5.48
Vacuole Periphery -2.18 3.08 0.19 1.79 -1.60 -2.84 0.11 1.23 3.81 1.16 -3.87 1.68 1.07 4.44 -0.23
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
ORF
Human Ortholog
Description Protein of unknown function that associates with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and bud; not an essential gene; protein abundance increases in response to DNA replication stress; YMR295C has a paralog, YGR273C, that arose from the whole genome duplication
Localization
Cell Percentages cell periphery (48%)
Cell Cycle Regulation No
Subcompartmental Group cell periphery-3

YMR295C

YMR295C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ymr295c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available