Standard name | |
---|---|
Human Ortholog | |
Description | Component of serine palmitoyltransferase; responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 13 | 29 | 65 | 49 | 65 | 53 | 63 | 54 | – | 56 | 85 | 90 | 23 | 29 | 16 | 29 | 49 | 28 | 3 | 5 | 19 | 18 | 24 | 19 |
Bud | 1 | 8 | 4 | 2 | 6 | 2 | 3 | 4 | – | 5 | 23 | 10 | 2 | 2 | 1 | 2 | 3 | 1 | 1 | 0 | 0 | 0 | 1 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 13 | 25 | 52 | 43 | 39 | 60 | 62 | 46 | – | 57 | 72 | 75 | 95 | 98 | 81 | 87 | 64 | 63 | 4 | 8 | 29 | 37 | 80 | 89 |
Cytoplasm | 14 | 25 | 18 | 26 | 30 | 36 | 51 | 70 | – | 50 | 67 | 68 | 41 | 27 | 43 | 30 | 48 | 19 | 2 | 5 | 1 | 1 | 4 | 3 |
Endoplasmic Reticulum | 140 | 165 | 287 | 184 | 300 | 319 | 390 | 439 | – | 325 | 258 | 321 | 348 | 398 | 245 | 2 | 2 | 5 | 150 | 196 | 287 | 349 | 450 | 432 |
Endosome | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 3 |
Golgi | 5 | 0 | 35 | 43 | 36 | 7 | 10 | 20 | – | 3 | 6 | 29 | 14 | 7 | 0 | 8 | 18 | 18 | 1 | 0 | 1 | 5 | 5 | 4 |
Mitochondria | 0 | 3 | 28 | 40 | 43 | 70 | 75 | 94 | – | 175 | 184 | 134 | 1 | 1 | 0 | 14 | 18 | 7 | 3 | 0 | 7 | 8 | 11 | 10 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 1 | 0 | 1 | 0 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 2 | 0 | 1 | 7 | 9 | 13 | – | 15 | 10 | 14 | 0 | 0 | 0 | 2 | 2 | 0 | 4 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 6 | 0 | 8 | 3 | 10 | 3 | 13 | 31 | – | 8 | 15 | 35 | 2 | 1 | 0 | 0 | 3 | 0 | 8 | 2 | 0 | 2 | 3 | 5 |
Unique Cell Count | 162 | 207 | 342 | 249 | 377 | 398 | 516 | 617 | 502 | 499 | 557 | 412 | 463 | 315 | 154 | 183 | 117 | 182 | 224 | 355 | 432 | 585 | 578 | |
Labelled Cell Count | 192 | 255 | 499 | 391 | 535 | 557 | 677 | 772 | 695 | 720 | 778 | 526 | 564 | 387 | 176 | 208 | 141 | 182 | 224 | 355 | 432 | 585 | 578 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 15.9 | 19.2 | 15.9 | 16.1 | 14.7 | 13.2 | 13.4 | 13.4 | – | 11.9 | 12.0 | 12.4 | 18.7 | 17.7 | 20.0 | 12.5 | 15.6 | 18.9 | 18.9 | 19.3 | 20.0 |
Std Deviation (1e-4) | 2.6 | 3.4 | 2.7 | 2.7 | 2.6 | 2.0 | 2.8 | 2.5 | – | 2.2 | 2.2 | 3.5 | 3.0 | 2.7 | 3.3 | 2.7 | 3.9 | 4.4 | 2.7 | 3.2 | 3.3 |
Intensity Change (Log2) | – | – | – | 0.02 | -0.11 | -0.27 | -0.25 | -0.24 | – | -0.41 | -0.41 | -0.35 | 0.24 | 0.16 | 0.34 | -0.34 | -0.02 | 0.25 | 0.25 | 0.28 | 0.33 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.2 | -0.6 | -2.1 | -2.7 | -4.6 | – | -3.2 | -0.7 | -1.1 | -5.7 | -5.6 | -5.4 | 0 | 2.1 | 1.1 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 2.8 | 0.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.7 | -2.0 | 0 | -1.3 | -3.8 | – | -1.6 | -0.3 | -0.7 | 2.7 | 2.1 | 3.3 | 9.5 | 5.2 | 8.3 |
Cytoplasm | 2.4 | 1.4 | 2.0 | 2.4 | 3.1 | – | 2.5 | 3.9 | 3.4 | 2.4 | 0.3 | 3.7 | 5.0 | 6.9 | 3.8 |
Endoplasmic Reticulum | -3.0 | -1.5 | -1.3 | -2.9 | -4.4 | – | -6.1 | -9.6 | -8.2 | 0.2 | 0.8 | -2.0 | -17.3 | -18.2 | -15.5 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 2.5 | -0.3 | -5.0 | -5.3 | -4.5 | – | -6.6 | -6.0 | -2.8 | -3.8 | -5.5 | -5.8 | -1.8 | -0.1 | 1.5 |
Mitochondria | 3.0 | 1.4 | 3.8 | 2.8 | 3.1 | – | 8.9 | 9.4 | 6.0 | -5.6 | -6.0 | -5.2 | 0.3 | 0.6 | -0.8 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 1.8 | – | 2.4 | 0 | 2.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0.3 | -1.8 | 0.2 | 2.0 | – | -0.8 | 0.6 | 2.7 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 18.5506 | 17.3618 | 17.5022 | 16.1258 | 15.8918 | 17.1057 | 16.933 | 19.6257 | 19.8742 | 19.4572 | 17.1963 | 19.9343 | 16.3232 | 19.3902 | 19.9514 | 17.5361 | 18.1425 | 19.1869 |
Actin | 0.0018 | 0.0034 | 0.0025 | 0.0013 | 0 | 0.0035 | 0.0928 | 0.0196 | 0.0323 | 0.0028 | 0.011 | 0.0108 | 0.0199 | 0.0056 | 0.0224 | 0.0532 | 0.0316 | 0.0088 |
Bud | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0.0005 | 0.0007 | 0.0006 | 0.0008 | 0.0002 | 0.0007 | 0.0001 | 0.0008 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0001 |
Bud Neck | 0.0003 | 0.0005 | 0.0018 | 0.0005 | 0.0001 | 0.0011 | 0.0012 | 0.0014 | 0.0058 | 0.0011 | 0.0136 | 0.0082 | 0.0015 | 0.0012 | 0.0016 | 0.0005 | 0.0053 | 0.0075 |
Bud Periphery | 0.0005 | 0.0006 | 0.0008 | 0.0002 | 0 | 0.0013 | 0.0014 | 0.0012 | 0.0071 | 0.0015 | 0.01 | 0.0006 | 0.0016 | 0.0006 | 0.0009 | 0.0002 | 0.0018 | 0.0004 |
Bud Site | 0.0035 | 0.0037 | 0.0009 | 0 | 0 | 0.0003 | 0.0021 | 0.0158 | 0.0062 | 0.0001 | 0.0006 | 0.0004 | 0.0141 | 0.0016 | 0.001 | 0.0002 | 0.0001 | 0.0001 |
Cell Periphery | 0.0257 | 0.0223 | 0.0311 | 0.0069 | 0.0102 | 0.0127 | 0.0112 | 0.0318 | 0.0372 | 0.0281 | 0.0283 | 0.0087 | 0.0258 | 0.0355 | 0.0205 | 0.006 | 0.0353 | 0.0088 |
Cytoplasm | 0.0186 | 0.0397 | 0.0195 | 0.0249 | 0.0016 | 0.0234 | 0.0234 | 0.0942 | 0.0197 | 0.0156 | 0.0406 | 0.0161 | 0.008 | 0.0349 | 0.0076 | 0.004 | 0.0343 | 0.0093 |
Cytoplasmic Foci | 0.0006 | 0.001 | 0.0019 | 0.0019 | 0.0001 | 0.0015 | 0.0092 | 0.0033 | 0.002 | 0.0009 | 0.0101 | 0.002 | 0.0068 | 0.0013 | 0.0097 | 0.0022 | 0.0015 | 0.0006 |
Eisosomes | 0.0025 | 0.002 | 0.0008 | 0.0002 | 0.0002 | 0.0006 | 0.0009 | 0.0005 | 0.0009 | 0.0008 | 0.0015 | 0.001 | 0.0012 | 0.0004 | 0.0003 | 0.0001 | 0.0007 | 0.0033 |
Endoplasmic Reticulum | 0.9227 | 0.8818 | 0.9142 | 0.9375 | 0.9757 | 0.9275 | 0.7023 | 0.771 | 0.8415 | 0.904 | 0.7677 | 0.921 | 0.7451 | 0.8605 | 0.8891 | 0.9007 | 0.8535 | 0.9455 |
Endosome | 0.0013 | 0.0027 | 0.0026 | 0.0041 | 0.0003 | 0.002 | 0.0311 | 0.007 | 0.0055 | 0.0063 | 0.0102 | 0.0051 | 0.0209 | 0.0039 | 0.0081 | 0.004 | 0.0035 | 0.002 |
Golgi | 0.0064 | 0.0085 | 0.0064 | 0.003 | 0.0005 | 0.005 | 0.0249 | 0.0109 | 0.0093 | 0.0222 | 0.0346 | 0.0119 | 0.0114 | 0.0048 | 0.009 | 0.0099 | 0.0157 | 0.0059 |
Lipid Particles | 0.0066 | 0.0172 | 0.0045 | 0.0119 | 0.0062 | 0.0108 | 0.0233 | 0.0172 | 0.0131 | 0.0033 | 0.0146 | 0.0047 | 0.0427 | 0.0323 | 0.0097 | 0.0022 | 0.0056 | 0.001 |
Mitochondria | 0.0002 | 0.0038 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0039 | 0.001 | 0.0007 | 0.0022 | 0.0335 | 0.0028 | 0.0723 | 0.0004 | 0.0023 | 0.0005 | 0.0002 | 0.0002 |
None | 0.0004 | 0.0005 | 0.0003 | 0.001 | 0 | 0.0008 | 0.0035 | 0.0003 | 0.0002 | 0.0003 | 0.0005 | 0.0001 | 0.0008 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Nuclear Periphery | 0.0052 | 0.0062 | 0.0065 | 0.0038 | 0.0029 | 0.0032 | 0.0382 | 0.01 | 0.0086 | 0.0059 | 0.004 | 0.0038 | 0.013 | 0.0108 | 0.0096 | 0.0116 | 0.0058 | 0.0045 |
Nucleolus | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0006 | 0 | 0.0001 | 0 | 0 | 0 |
Nucleus | 0.0002 | 0.0009 | 0.0006 | 0.0002 | 0.0001 | 0.0008 | 0.0072 | 0.0012 | 0.0005 | 0.0003 | 0.0005 | 0.0003 | 0.0016 | 0.0006 | 0.0003 | 0.0008 | 0.0003 | 0.0002 |
Peroxisomes | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0105 | 0.0002 | 0.0003 | 0 | 0.0105 | 0.0002 | 0.0021 | 0 | 0.0032 | 0.0003 | 0 | 0 |
Punctate Nuclear | 0 | 0.0004 | 0.0001 | 0.0001 | 0 | 0.0004 | 0.0037 | 0.0002 | 0.0001 | 0 | 0.001 | 0.0001 | 0.0011 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0001 |
Vacuole | 0.0031 | 0.0038 | 0.0044 | 0.0022 | 0.0017 | 0.0041 | 0.0051 | 0.0117 | 0.0067 | 0.0039 | 0.0054 | 0.0018 | 0.0071 | 0.0045 | 0.0029 | 0.0027 | 0.0037 | 0.0011 |
Vacuole Periphery | 0.0002 | 0.0004 | 0.0004 | 0.0002 | 0.0001 | 0.0002 | 0.0029 | 0.0006 | 0.0013 | 0.0006 | 0.0011 | 0.0004 | 0.0017 | 0.0005 | 0.0009 | 0.0005 | 0.0005 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 155.2793 | 86.4623 | 77.6888 | 98.6878 | 97.6775 | 110.4766 | 96.4912 | 82.2844 | 85.0655 | 112.1828 |
Translational Efficiency | 0.8671 | 0.8414 | 0.9795 | 0.7953 | 0.8895 | 1.0173 | 0.6251 | 0.7385 | 0.73 | 0.7111 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
120 | 895 | 203 | 418 | 2137 | 874 | 2259 | 1577 | 2257 | 1769 | 2462 | 1995 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1678.72 | 1449.43 | 1948.75 | 2123.04 | 1340.89 | 1351.45 | 1923.70 | 1930.45 | 1358.85 | 1401.02 | 1925.77 | 1970.80 |
Standard Deviation | 230.44 | 252.77 | 263.44 | 342.27 | 208.98 | 221.85 | 258.70 | 322.95 | 223.43 | 242.99 | 259.18 | 336.35 |
Intensity Change Log 2 | — | -0.211876 | 0.215187 | 0.338770 | — | 0.011317 | 0.520693 | 0.525746 | — | -0.108482 | 0.358884 | 0.424802 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.002855 | 0.000977 | 0.000506 | 0.002428 | 0.001033 | 0.001190 | 0.001469 | 0.002752 | 0.001130 | 0.001082 | 0.001390 | 0.002684 |
Bud Neck | 0.010005 | 0.000413 | 0.001645 | 0.007921 | 0.000435 | 0.000472 | 0.000580 | 0.001659 | 0.000944 | 0.000442 | 0.000668 | 0.002971 |
Bud Site | 0.016401 | 0.003807 | 0.001310 | 0.025243 | 0.002743 | 0.004481 | 0.003549 | 0.009663 | 0.003470 | 0.004140 | 0.003364 | 0.012928 |
Cell Periphery | 0.069744 | 0.025778 | 0.064498 | 0.107464* | 0.037364 | 0.045007 | 0.054892 | 0.100090* | 0.039086 | 0.035278 | 0.055684 | 0.101635* |
Cytoplasm | 0.004898 | 0.001847 | 0.000037 | 0.004323 | 0.001261 | 0.001879 | 0.002324 | 0.003828 | 0.001454 | 0.001863 | 0.002136 | 0.003932 |
Cytoplasmic Foci | 0.006391 | 0.003809 | 0.000088 | 0.004560 | 0.003207 | 0.005121 | 0.001724 | 0.003916 | 0.003377 | 0.004457 | 0.001589 | 0.004051 |
Eisosomes | 0.000616 | 0.000556 | 0.001304 | 0.001261 | 0.000962 | 0.000709 | 0.001210 | 0.000912 | 0.000944 | 0.000632 | 0.001218 | 0.000985 |
Endoplasmic Reticulum | 0.657398* | 0.936357* | 0.902472* | 0.629902* | 0.922436* | 0.916583* | 0.883748* | 0.761509* | 0.908344* | 0.926588* | 0.885292* | 0.733934* |
Endosome | 0.020659 | 0.005831 | 0.003852 | 0.013742 | 0.004612 | 0.005249 | 0.004099 | 0.008352 | 0.005465 | 0.005543 | 0.004079 | 0.009481 |
Golgi | 0.021571 | 0.004242 | 0.007939 | 0.018938 | 0.003864 | 0.004230 | 0.004371 | 0.011780 | 0.004806 | 0.004236 | 0.004665 | 0.013280 |
Lipid Particles | 0.088758 | 0.008645 | 0.004039 | 0.135336* | 0.006526 | 0.006741 | 0.018675 | 0.065207 | 0.010898 | 0.007704 | 0.017468 | 0.079900 |
Mitochondria | 0.001214 | 0.001513 | 0.000066 | 0.000280 | 0.001914 | 0.000839 | 0.001375 | 0.001505 | 0.001877 | 0.001180 | 0.001267 | 0.001248 |
Mitotic Spindle | 0.000917 | 0.000106 | 0.000107 | 0.002099 | 0.000476 | 0.000375 | 0.000311 | 0.001015 | 0.000500 | 0.000239 | 0.000294 | 0.001242 |
None | 0.000015 | 0.000197 | 0.000007 | 0.000088 | 0.000602 | 0.000096 | 0.000585 | 0.001167 | 0.000571 | 0.000147 | 0.000537 | 0.000941 |
Nuclear Periphery | 0.001476 | 0.000589 | 0.001624 | 0.005119 | 0.001141 | 0.000591 | 0.003867 | 0.005012 | 0.001159 | 0.000590 | 0.003682 | 0.005035 |
Nuclear Periphery Foci | 0.020595 | 0.000392 | 0.000907 | 0.009233 | 0.000288 | 0.000576 | 0.000515 | 0.003251 | 0.001368 | 0.000483 | 0.000548 | 0.004504 |
Nucleolus | 0.000013 | 0.000025 | 0.000003 | 0.000016 | 0.000022 | 0.000049 | 0.000027 | 0.000025 | 0.000021 | 0.000037 | 0.000025 | 0.000023 |
Nucleus | 0.000724 | 0.000133 | 0.000035 | 0.000275 | 0.000201 | 0.000242 | 0.000237 | 0.000255 | 0.000229 | 0.000187 | 0.000220 | 0.000259 |
Peroxisomes | 0.000346 | 0.002275 | 0.000021 | 0.000219 | 0.009304 | 0.002871 | 0.009078 | 0.003121 | 0.008827 | 0.002569 | 0.008331 | 0.002513 |
Vacuole | 0.062071 | 0.001603 | 0.008652 | 0.028546 | 0.000774 | 0.001441 | 0.006180 | 0.012008 | 0.004033 | 0.001523 | 0.006384 | 0.015473 |
Vacuole Periphery | 0.013333 | 0.000906 | 0.000889 | 0.003007 | 0.000834 | 0.001259 | 0.001183 | 0.002972 | 0.001499 | 0.001080 | 0.001159 | 0.002979 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 2.05 | 2.45 | 0.82 | -2.94 | -4.13 | -0.31 | -0.78 | -2.23 | -2.24 | -1.69 | 0.19 | -0.43 | -2.32 | -2.93 | -2.06 |
Bud Neck | 3.81 | 3.32 | 0.94 | -3.77 | -3.04 | -0.28 | -1.24 | -4.00 | -3.90 | -3.49 | 3.00 | 1.68 | -3.84 | -5.22 | -4.70 |
Bud Site | 2.81 | 3.33 | -1.13 | -4.48 | -5.03 | -1.28 | -0.89 | -4.33 | -2.93 | -3.87 | -0.51 | 0.18 | -5.62 | -5.33 | -5.90 |
Cell Periphery | 3.14 | 0.24 | -2.35 | -8.73 | -3.14 | -1.92 | -5.79 | -13.15 | -9.88 | -9.17 | 1.30 | -5.51 | -14.21 | -14.92 | -10.09 |
Cytoplasm | 2.53 | 4.20 | 0.41 | -1.93 | -3.49 | -1.43 | -2.38 | -4.72 | -3.49 | -2.53 | -0.89 | -1.56 | -4.71 | -3.84 | -3.24 |
Cytoplasmic Foci | 1.11 | 2.78 | 0.67 | -0.41 | -2.59 | -1.60 | 2.24 | -0.75 | 1.00 | -3.00 | -1.15 | 2.81 | -0.73 | 0.41 | -3.52 |
Eisosomes | 0.24 | -1.68 | -1.46 | -1.74 | 0.10 | 1.44 | -1.10 | 0.34 | -1.29 | 1.42 | 2.24 | -1.31 | -0.25 | -2.61 | 1.13 |
Endoplasmic Reticulum | -8.53 | -7.13 | 0.53 | 17.45 | 12.95 | 0.90 | 7.49 | 20.51 | 17.42 | 15.01 | -3.66 | 4.16 | 22.38 | 25.37 | 19.57 |
Endosome | 3.70 | 4.17 | 1.64 | -5.75 | -6.83 | -1.24 | 1.61 | -6.47 | -4.55 | -7.62 | -0.15 | 3.74 | -6.85 | -6.50 | -10.25 |
Golgi | 1.90 | 1.44 | 0.24 | -5.77 | -3.25 | -0.54 | -1.19 | -8.19 | -6.74 | -7.36 | 0.85 | 0.13 | -8.06 | -9.29 | -8.69 |
Lipid Particles | 5.06 | 5.33 | -2.14 | -10.91 | -11.27 | -0.19 | -7.46 | -15.76 | -15.45 | -11.75 | 2.48 | -3.54 | -17.20 | -18.49 | -15.34 |
Mitochondria | -0.27 | 1.29 | 1.04 | 1.45 | -2.92 | 1.64 | 0.84 | 0.55 | -1.27 | -0.25 | 0.96 | 1.00 | 0.94 | -0.12 | 0.05 |
Mitotic Spindle | 2.46 | 2.46 | -1.12 | -3.11 | -3.10 | 0.46 | 1.05 | -2.14 | -2.51 | -3.65 | 1.62 | 1.48 | -2.87 | -4.58 | -4.65 |
None | -2.39 | 1.46 | -4.91 | 1.36 | -6.00 | 2.45 | 0.15 | -0.74 | -1.51 | -0.81 | 2.18 | 0.23 | -0.59 | -1.42 | -0.71 |
Nuclear Periphery | 2.98 | -0.28 | -5.57 | -7.88 | -5.35 | 5.22 | -13.34 | -12.41 | -14.04 | -3.17 | 6.92 | -13.21 | -13.99 | -16.27 | -4.16 |
Nuclear Periphery Foci | 3.69 | 3.60 | 1.95 | -4.19 | -3.95 | -2.00 | -3.90 | -4.02 | -3.59 | -3.71 | 2.87 | 2.69 | -3.90 | -5.49 | -5.41 |
Nucleolus | -0.52 | 3.02 | -0.48 | 0.42 | -5.62 | -0.60 | -0.50 | -0.26 | 0.56 | 0.65 | -0.58 | -0.36 | -0.12 | 0.58 | 0.67 |
Nucleus | 1.98 | 2.18 | 1.67 | -2.14 | -6.20 | -0.20 | -0.45 | -0.77 | -0.09 | -0.47 | 0.73 | 0.59 | -0.05 | -0.93 | -1.30 |
Peroxisomes | -3.36 | 2.77 | 1.05 | 3.66 | -4.14 | 6.12 | 0.19 | 6.00 | -0.34 | 6.37 | 6.64 | 0.45 | 6.70 | 0.11 | 7.02 |
Vacuole | 5.09 | 4.50 | 2.68 | -7.94 | -5.65 | -2.27 | -11.22 | -14.23 | -12.66 | -6.29 | 3.54 | -2.68 | -9.55 | -14.25 | -8.63 |
Vacuole Periphery | 1.70 | 1.70 | 1.41 | -4.66 | -4.31 | -0.91 | -1.36 | -4.02 | -2.50 | -3.11 | 0.86 | 0.68 | -2.59 | -3.94 | -3.82 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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