Standard name
Human Ortholog
Description Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0.08 0 0 0 0 0 0
Cytoplasm 0.7 0.96 0.95 0.94 0.96 0.91 0.84 0.89 0.79 0.78 0.7 0.81 0.95 0.95 0.95 0.68 0.67 0.62 0.64 0.77 0.59 0.73 0.7 0.75
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.17 0.16 0.17 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.3 0 0 0.05 0 0.12 0.23 0.13 0.37 0.24 0.49 0.26 0 0 0 0 0 0.06 0.19 0.11 0.24 0.12 0.13 0.08
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.1 0.08 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 2 1 2 1 0 1 2 0 2 1 1 7 8 1 3 4 6 2 2 2 7 13 11
Bud 0 1 1 7 3 1 10 14 10 8 13 17 2 1 0 1 2 4 1 0 0 2 6 10
Bud Neck 1 0 1 4 16 5 7 9 6 7 6 10 14 17 11 1 2 2 1 2 0 4 1 6
Bud Site 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 1 0 2 5 2 4 3 0 2 4 3 2 0 1 9 8 12 0 0 0 2 3 9
Cytoplasm 58 129 57 182 329 239 291 336 214 222 237 327 372 499 618 123 80 94 88 122 55 217 254 363
Endoplasmic Reticulum 1 1 0 0 0 2 1 0 0 1 2 0 2 0 3 11 3 9 2 1 0 2 1 2
Endosome 1 0 0 0 0 1 0 0 0 0 1 0 1 1 5 30 19 26 6 2 0 6 4 9
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 3 2 0 0 2 1 4
Mitochondria 25 4 1 9 4 32 81 51 100 69 165 107 0 0 0 8 1 9 26 17 22 34 48 38
Nucleus 2 0 1 0 1 0 0 1 0 7 3 3 1 0 3 0 1 0 0 0 0 0 1 0
Nuclear Periphery 5 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 1 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 2 3 1 0 0 0 0 0 0
SpindlePole 3 0 0 5 7 7 3 8 6 2 3 11 7 18 23 20 12 12 3 0 0 3 14 12
Vac/Vac Membrane 0 1 0 0 0 0 3 0 4 1 2 5 0 0 1 10 6 6 3 5 3 5 2 5
Unique Cell Count 83 134 60 194 343 264 346 379 271 284 337 404 391 526 653 181 119 151 140 159 93 299 365 482
Labelled Cell Count 100 139 62 212 366 289 402 427 341 323 437 484 408 544 667 219 141 185 140 159 93 299 365 482


Cytoplasm, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 4.4 4.9 4.6 4.7 3.8 3.5 4.0 3.4 3.5 3.2 3.6 5.2 5.4 5.3 6.3 6.8 6.4 4.9 5.2 5.4
Std Deviation (1e-4) 3.6 0.6 1.8 1.7 1.4 0.8 1.1 1.1 1.2 1.1 0.9 0.8 1.0 1.0 1.1 1.5 1.8 1.6 1.1 1.3 1.5
Intensity Change (Log2) – – – -0.1 -0.06 -0.36 -0.47 -0.31 -0.52 -0.47 -0.62 -0.46 0.09 0.13 0.1 0.37 0.47 0.39 0.0 0.08 0.15


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.5235 -0.8564 -0.6392 -0.477 -0.0945 -0.8943 -0.5106 -0.0458 -0.186 -0.036 -0.5614 0.0329 -1.4569 -1.1405 -1.318 -0.9592 -1.5798 -1.4314
Actin 0.0215 0.0043 0.0185 0.0143 0.0416 0.0089 0.0141 0.0062 0.0151 0.0118 0.0188 0.0128 0.0176 0.0036 0.023 0.0027 0.0065 0.0005
Bud 0.0007 0.0046 0.0043 0.0013 0.0178 0.0009 0.0019 0.003 0.0023 0.009 0.0167 0.0005 0.0006 0.0016 0.0076 0.0002 0.0005 0
Bud Neck 0.0011 0.0003 0.0114 0.1209 0.0027 0.0003 0.0012 0.0071 0.0267 0.0255 0.0011 0.0004 0.0009 0.0004 0.0128 0.0525 0.0004 0.0004
Bud Periphery 0.0008 0.0064 0.0072 0.0024 0.0082 0.0032 0.0041 0.0025 0.0047 0.0195 0.0046 0.0075 0.0009 0.0011 0.002 0.0002 0.0006 0
Bud Site 0.0116 0.0115 0.0145 0.0048 0.0091 0.0001 0.0036 0.0162 0.0108 0.0051 0.0021 0.0001 0.0016 0.0188 0.0157 0.0083 0.0007 0
Cell Periphery 0.0002 0.0003 0.0004 0.0002 0.0006 0.0001 0.0006 0.0008 0.0002 0.0002 0.0004 0.0003 0.0003 0.0003 0.0004 0.0002 0.0001 0
Cytoplasm 0.0716 0.1129 0.1098 0.091 0.1039 0.1542 0.1297 0.1663 0.1582 0.1734 0.2107 0.2285 0.1979 0.3609 0.2599 0.3561 0.29 0.3919
Cytoplasmic Foci 0.0469 0.026 0.0478 0.1197 0.0715 0.0352 0.0363 0.0216 0.0585 0.134 0.0466 0.0203 0.0697 0.0239 0.1014 0.1769 0.1881 0.042
Eisosomes 0.0002 0.0002 0.0003 0.0002 0.0005 0.0003 0.0003 0.0001 0.0002 0.0001 0.0001 0.0009 0.0003 0.0003 0.0007 0.0001 0.0002 0.0001
Endoplasmic Reticulum 0.0022 0.0015 0.0046 0.002 0.0045 0.001 0.0042 0.0012 0.0019 0.0006 0.0008 0.0012 0.0046 0.0031 0.0041 0.0027 0.0062 0.0032
Endosome 0.0527 0.0048 0.0245 0.0423 0.0754 0.0069 0.0309 0.0044 0.0101 0.0123 0.0148 0.0146 0.0326 0.0139 0.0211 0.0243 0.0958 0.0118
Golgi 0.0156 0.0008 0.0039 0.0283 0.0265 0.0008 0.0098 0.0006 0.0043 0.0041 0.0055 0.0041 0.009 0.0014 0.0038 0.0042 0.0082 0.0004
Lipid Particles 0.0079 0.0013 0.0057 0.0168 0.0461 0.0019 0.0116 0.0002 0.0051 0.0114 0.0129 0.0042 0.0116 0.0008 0.0071 0.0207 0.015 0.0004
Mitochondria 0.0037 0.0012 0.0049 0.0287 0.0172 0.0009 0.0043 0.0008 0.0091 0.009 0.0033 0.003 0.0065 0.0009 0.0027 0.001 0.0048 0.0004
None 0.707 0.813 0.7045 0.463 0.4848 0.7765 0.6961 0.7585 0.67 0.5267 0.6438 0.6877 0.5945 0.561 0.5049 0.2986 0.311 0.5446
Nuclear Periphery 0.0154 0.0008 0.0058 0.0014 0.0224 0.001 0.0135 0.0005 0.0012 0.0005 0.0007 0.0037 0.0134 0.0008 0.008 0.0006 0.0125 0.0003
Nucleolus 0.0015 0.0004 0.001 0.0012 0.0017 0.0002 0.0011 0.0005 0.0006 0.0004 0.0003 0.0003 0.0014 0.0002 0.0012 0.0002 0.0003 0.0001
Nucleus 0.0085 0.0014 0.0039 0.0015 0.0079 0.0006 0.0063 0.0023 0.002 0.0009 0.0044 0.0055 0.0072 0.0011 0.0043 0.0006 0.0011 0.0006
Peroxisomes 0.02 0.0024 0.0142 0.0513 0.0262 0.0041 0.0119 0.0016 0.0103 0.0517 0.009 0.0012 0.0212 0.0009 0.0111 0.0442 0.047 0.0007
Punctate Nuclear 0.0038 0.0035 0.0072 0.004 0.0238 0.002 0.0052 0.004 0.0068 0.0016 0.0017 0.0019 0.0042 0.0015 0.0048 0.004 0.0034 0.0011
Vacuole 0.0047 0.0021 0.0042 0.0036 0.0049 0.0008 0.0086 0.0014 0.0015 0.0018 0.0015 0.001 0.0027 0.003 0.0027 0.0013 0.0053 0.0011
Vacuole Periphery 0.0026 0.0003 0.0014 0.0011 0.0027 0.0003 0.0047 0.0001 0.0004 0.0004 0.0003 0.0004 0.0014 0.0003 0.0008 0.0002 0.0022 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.9882 18.9082 7.7948 10.821 12.9502 8.221 16.9938 12.9034 11.7871 13.4942
Translational Efficiency 0.6805 0.3759 0.5811 0.4796 0.3745 0.6922 0.5643 0.6119 0.4913 0.4971

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1385 550 1686 942 2192 1093 177 1231 3577 1643 1863 2173

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 670.80 728.11 790.57 794.71 671.00 723.80 915.78 870.73 670.92 725.24 802.47 837.78
Standard Deviation 78.97 95.45 104.06 106.77 74.87 100.08 100.16 113.57 76.48 98.57 110.00 116.91
Intensity Change Log 2 — 0.118274 0.237011 0.244546 — 0.109278 0.448688 0.375913 — 0.113782 0.346744 0.311734

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000328 0.001013 0.000315 0.001462 0.000292 0.000480 0.000378 0.001103 0.000306 0.000658 0.000321 0.001259
Bud Neck 0.025484 0.032814 0.007648 0.018015 0.031792 0.047969 0.003568 0.012782 0.029349 0.042896 0.007260 0.015050
Bud Site 0.006822 0.025240 0.011550 0.052617 0.011022 0.022837 0.016978 0.057060 0.009396 0.023641 0.012066 0.055134
Cell Periphery 0.000134 0.000193 0.000123 0.000179 0.000211 0.000268 0.000411 0.000129 0.000181 0.000243 0.000150 0.000150
Cytoplasm 0.378295* 0.305298* 0.512370* 0.424697* 0.412030* 0.276290* 0.399876* 0.481091* 0.398968* 0.286001* 0.501683* 0.456644*
Cytoplasmic Foci 0.375536* 0.393582* 0.032446 0.117137 0.297778* 0.302801* 0.103651 0.073170 0.327885* 0.333190* 0.039211 0.092230
Eisosomes 0.000260 0.000196 0.000040 0.000104 0.000292 0.000225 0.000172 0.000061 0.000280 0.000216 0.000053 0.000079
Endoplasmic Reticulum 0.001010 0.001019 0.002602 0.001485 0.003187 0.001807 0.003650 0.001197 0.002344 0.001543 0.002702 0.001322
Endosome 0.036501 0.052751 0.003689 0.028094 0.026356 0.066817 0.014735 0.016096 0.030284 0.062109 0.004738 0.021298
Golgi 0.007856 0.014036 0.000193 0.000925 0.011220 0.011554 0.000221 0.003417 0.009918 0.012385 0.000196 0.002336
Lipid Particles 0.015983 0.010203 0.002118 0.003772 0.018058 0.013723 0.007797 0.001612 0.017254 0.012544 0.002658 0.002548
Mitochondria 0.008217 0.014419 0.000688 0.005217 0.010423 0.017547 0.000361 0.007919 0.009569 0.016500 0.000657 0.006748
Mitotic Spindle 0.001731 0.005161 0.007281 0.023608 0.004109 0.009842 0.000857 0.048646 0.003189 0.008275 0.006671 0.037792
None 0.001545 0.001091 0.007528 0.002477 0.002745 0.002668 0.010017 0.002714 0.002280 0.002140 0.007765 0.002611
Nuclear Periphery 0.000364 0.000323 0.001422 0.000942 0.000465 0.000640 0.001624 0.000941 0.000426 0.000534 0.001441 0.000941
Nuclear Periphery Foci 0.000437 0.000751 0.002385 0.002797 0.000363 0.000496 0.009774 0.001564 0.000392 0.000581 0.003087 0.002098
Nucleolus 0.001055 0.001323 0.001038 0.001979 0.001363 0.002594 0.001449 0.000875 0.001244 0.002169 0.001077 0.001354
Nucleus 0.060816 0.031998 0.354429* 0.062520 0.092659 0.058708 0.245997* 0.079795 0.080329 0.049766 0.344127* 0.072306
Peroxisomes 0.014723 0.025292 0.001995 0.025016 0.009307 0.024723 0.001550 0.014383 0.011404 0.024913 0.001953 0.018992
Vacuole 0.061032 0.079576 0.049893 0.224940* 0.063935 0.132069 0.176563 0.188608 0.062811 0.114497 0.061928 0.204358*
Vacuole Periphery 0.001870 0.003721 0.000246 0.002019 0.002393 0.005941 0.000370 0.006837 0.002191 0.005198 0.000258 0.004748

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.21 -1.51 -4.60 -1.05 -4.08 -2.27 -2.68 -3.32 -1.53 -1.55 -3.91 -2.27 -5.67 -2.16 -4.31
Bud Neck -1.79 8.53 5.08 4.79 -2.91 -4.64 9.91 10.70 12.20 -1.78 -5.07 13.47 11.51 12.66 -2.35
Bud Site -8.18 -3.16 -8.12 -2.49 -6.33 -4.89 -0.28 -6.23 -1.63 -2.76 -8.10 -1.52 -10.00 -2.93 -7.81
Cell Periphery -1.17 0.89 -0.12 1.11 -0.74 -0.98 -2.05 5.57 3.47 4.74 -1.48 2.42 4.81 3.47 3.68
Cytoplasm 5.39 -8.78 0.88 -4.15 8.29 14.19 4.35 2.07 -10.21 -3.17 14.47 -6.74 2.33 -10.60 7.86
Cytoplasmic Foci -1.37 50.61 34.21 25.88 -13.15 -0.44 22.16 43.89 33.06 3.18 -0.55 64.88 53.61 40.08 -10.08
Eisosomes 2.09 16.50 11.95 3.40 -8.54 1.76 5.99 15.88 4.39 8.12 2.36 21.46 19.56 5.21 -4.89
Endoplasmic Reticulum -0.02 -9.87 -3.44 -3.64 7.59 2.55 -2.88 2.82 -0.01 6.39 2.33 -3.80 1.98 -0.98 8.89
Endosome -3.80 15.88 5.05 7.29 -8.70 -11.79 4.31 11.31 17.46 3.03 -11.78 21.46 10.35 17.80 -8.92
Golgi -2.97 10.75 10.13 7.17 -6.07 -0.12 15.19 12.66 8.25 -2.68 -1.98 18.59 16.19 11.02 -3.68
Lipid Particles 3.84 13.49 11.85 5.37 -3.72 2.78 7.27 16.74 9.77 5.42 4.14 19.68 20.33 10.90 0.59
Mitochondria -2.13 6.89 3.30 4.00 -1.65 -3.60 11.40 5.77 7.36 -2.39 -4.25 12.90 6.57 8.32 -2.62
Mitotic Spindle -2.67 -4.85 -6.56 -5.07 -4.04 -2.56 1.91 -9.15 -6.80 -9.83 -3.36 -3.73 -11.17 -8.10 -8.33
None 1.37 -5.72 -1.44 -2.50 4.33 0.11 -1.43 -1.12 -0.74 1.23 0.29 -5.24 -1.64 -1.31 4.45
Nuclear Periphery 0.47 -14.01 -7.30 -8.08 4.02 -1.90 -7.99 -12.33 -10.66 -0.47 -1.67 -16.05 -14.16 -12.58 -0.03
Nuclear Periphery Foci -1.85 -7.23 -7.63 -5.42 0.90 -1.68 -3.93 -8.10 -7.46 3.21 -2.44 -8.15 -11.15 -9.45 3.01
Nucleolus -1.46 -2.70 -2.41 -1.20 -1.01 -2.34 -0.96 0.05 2.54 1.12 -2.74 -1.87 -1.81 1.30 -0.06
Nucleus 8.42 -42.63 -0.58 -7.37 39.91 7.76 -8.97 -4.81 -10.54 7.39 9.86 -41.37 -3.82 -11.49 37.42
Peroxisomes -3.87 9.43 -1.09 2.55 -7.23 -7.47 9.42 1.45 7.45 -4.06 -8.13 12.60 -0.18 6.92 -7.81
Vacuole -3.45 -3.12 -27.98 -22.79 -26.15 -11.72 -10.58 -30.91 -19.42 -2.43 -11.97 -7.42 -41.64 -28.41 -33.00
Vacuole Periphery -3.55 12.35 1.95 4.19 -2.49 -6.56 11.14 0.30 3.96 -2.32 -7.55 15.70 0.98 5.67 -3.08
Standard name
Human Ortholog
Description Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration
Localization
Cell Percentages cytoplasm (39%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Dyn3

Dyn3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dyn3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available