Standard name | |
---|---|
Human Ortholog | |
Description | Ubiquitin-specific protease involved in protein deubiquitination; forms a complex with AAA peroxins Pex1p and Pex6p; deubiquitinates mono- and polyubiquitinated forms of Pex5p; deubiquitinates Clb5p, counteracting APC activity, and facilitating both Clb5p accumulation and S phase entry; physically interacts with anaphase-promoting complex/cyclosome (APC/C) activator, Cdh1p; catalytic activity regulated by an N-terminal TRAF-like domain and and C-terminal sequences |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 18 | 2 | 5 | 1 | 3 | 1 | 3 | 5 | 2 | – | 6 | 3 | 0 | 0 | 0 | 0 | 2 | 1 | 10 | 7 | 8 | 0 | 1 | 0 |
Bud | 3 | 1 | 10 | 1 | 1 | 3 | 14 | 4 | 6 | – | 7 | 13 | 3 | 1 | 1 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 3 | 2 | 3 | 3 | 0 | 4 | 2 | 12 | – | 8 | 11 | 1 | 1 | 0 | 3 | 2 | 2 | 0 | 0 | 0 | 1 | 0 | 0 |
Cytoplasm | 157 | 208 | 201 | 215 | 244 | 260 | 372 | 348 | 224 | – | 192 | 227 | 151 | 202 | 216 | 267 | 204 | 237 | 117 | 182 | 181 | 70 | 169 | 150 |
Endoplasmic Reticulum | 22 | 12 | 10 | 5 | 8 | 21 | 21 | 14 | 25 | – | 20 | 32 | 2 | 2 | 2 | 10 | 2 | 15 | 6 | 2 | 3 | 1 | 3 | 3 |
Endosome | 26 | 7 | 8 | 5 | 8 | 2 | 12 | 13 | 3 | – | 4 | 4 | 1 | 0 | 0 | 2 | 10 | 5 | 85 | 42 | 31 | 3 | 11 | 6 |
Golgi | 1 | 1 | 1 | 0 | 0 | 2 | 0 | 1 | 2 | – | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 11 | 0 | 4 | 1 | 0 | 0 |
Mitochondria | 0 | 7 | 5 | 23 | 16 | 53 | 100 | 85 | 98 | – | 131 | 162 | 0 | 3 | 6 | 4 | 0 | 3 | 4 | 9 | 19 | 1 | 3 | 1 |
Nucleus | 0 | 0 | 1 | 0 | 0 | 2 | 6 | 3 | 0 | – | 4 | 2 | 0 | 0 | 4 | 11 | 1 | 3 | 0 | 1 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | – | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 1 |
Nucleolus | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 12 | 3 | 5 | 0 | 3 | 2 |
Vac/Vac Membrane | 6 | 6 | 11 | 4 | 16 | 5 | 18 | 41 | 14 | – | 33 | 37 | 0 | 2 | 1 | 33 | 69 | 17 | 2 | 9 | 20 | 4 | 6 | 1 |
Unique Cell Count | 214 | 234 | 239 | 243 | 280 | 321 | 493 | 484 | 335 | 342 | 407 | 154 | 206 | 225 | 295 | 251 | 253 | 259 | 271 | 285 | 88 | 207 | 173 | |
Labelled Cell Count | 239 | 248 | 254 | 257 | 299 | 350 | 551 | 520 | 387 | 408 | 493 | 158 | 211 | 230 | 331 | 291 | 283 | 259 | 271 | 285 | 88 | 207 | 173 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.8 | 7.6 | 6.7 | 6.5 | 6.8 | 7.0 | 7.1 | 7.2 | 6.9 | – | 7.0 | 7.1 | 8.0 | 8.6 | 7.8 | 12.4 | 15.0 | 14.0 | 7.8 | 8.2 | 9.1 |
Std Deviation (1e-4) | 1.3 | 1.3 | 1.1 | 1.2 | 1.3 | 1.2 | 1.5 | 1.5 | 1.7 | – | 1.8 | 2.0 | 1.8 | 1.6 | 1.4 | 2.7 | 3.1 | 3.3 | 1.5 | 1.6 | 2.3 |
Intensity Change (Log2) | – | – | – | -0.03 | 0.03 | 0.07 | 0.09 | 0.11 | 0.06 | – | 0.07 | 0.09 | 0.27 | 0.36 | 0.23 | 0.9 | 1.17 | 1.07 | 0.23 | 0.3 | 0.45 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | -2.8 | -3.0 | -2.5 | -1.0 | 0 | -1.7 | – | -1.5 | -0.7 | -1.2 | -2.5 | -2.6 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 2.1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.4 | 1.0 | -1.0 | -2.7 | -3.6 | -4.6 | – | -7.1 | -7.4 | 4.4 | 5.0 | 4.2 | 2.2 | -0.8 | 3.4 |
Endoplasmic Reticulum | -1.3 | -0.8 | 1.2 | 0 | -0.9 | 1.6 | – | 0.9 | 1.8 | 0 | -2.1 | -2.2 | -0.5 | -2.4 | 0.9 |
Endosome | -0.9 | -0.3 | 0 | -0.7 | -0.5 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0.4 | -0.9 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.5 | 2.1 | 5.5 | 6.6 | 5.9 | 8.4 | – | 10.1 | 10.6 | 0 | 0 | 0.4 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2.6 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.9 | 0.6 | -2.1 | -0.6 | 1.9 | -0.2 | – | 2.3 | 2.1 | 0 | -2.3 | -2.8 | 2.8 | 6.9 | 1.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.7936 | 9.9597 | 8.8052 | 8.3151 | 8.3153 | 9.1267 | 4.7988 | 7.2042 | 6.6263 | 5.8215 | 5.1909 | 6.419 | 5.5811 | 6.5463 | 5.9609 | 5.7522 | 4.8681 | 6.256 |
Actin | 0.0117 | 0.0019 | 0.0133 | 0.0024 | 0.0021 | 0.0079 | 0.0398 | 0.0071 | 0.0079 | 0.0203 | 0.0027 | 0.0025 | 0.0016 | 0.0024 | 0.0079 | 0.0034 | 0.0106 | 0.0032 |
Bud | 0.0005 | 0.0004 | 0.0007 | 0.0007 | 0.0004 | 0.0003 | 0.0004 | 0.0003 | 0.0042 | 0.0003 | 0.0003 | 0.0049 | 0.0003 | 0.0003 | 0.0003 | 0.0007 | 0.0002 | 0.0002 |
Bud Neck | 0.0025 | 0.001 | 0.0017 | 0.0026 | 0.0039 | 0.0051 | 0.01 | 0.0004 | 0.0014 | 0.0007 | 0.0009 | 0.0041 | 0.0003 | 0.0005 | 0.0004 | 0.0018 | 0.0011 | 0.0073 |
Bud Periphery | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0015 | 0.001 | 0.0002 | 0.002 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 |
Bud Site | 0.0033 | 0.0035 | 0.0045 | 0.0007 | 0.0016 | 0.0004 | 0.017 | 0.0072 | 0.0104 | 0.0007 | 0.001 | 0.0013 | 0.0013 | 0.0029 | 0.0008 | 0.0008 | 0.0012 | 0.0001 |
Cell Periphery | 0.0005 | 0.0004 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.7768 | 0.9035 | 0.8179 | 0.8268 | 0.847 | 0.855 | 0.5992 | 0.837 | 0.778 | 0.6782 | 0.6592 | 0.7841 | 0.7605 | 0.8032 | 0.8313 | 0.7551 | 0.5995 | 0.7728 |
Cytoplasmic Foci | 0.078 | 0.0393 | 0.0704 | 0.0836 | 0.0612 | 0.0599 | 0.1226 | 0.1023 | 0.1044 | 0.1313 | 0.1644 | 0.1242 | 0.0727 | 0.09 | 0.0753 | 0.1187 | 0.2138 | 0.0957 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0208 | 0.0082 | 0.0079 | 0.0039 | 0.009 | 0.0147 | 0.0091 | 0.0028 | 0.003 | 0.003 | 0.0035 | 0.0029 | 0.0079 | 0.0022 | 0.0058 | 0.0028 | 0.0013 | 0.003 |
Endosome | 0.0505 | 0.0185 | 0.0412 | 0.0399 | 0.047 | 0.0198 | 0.0742 | 0.0275 | 0.0397 | 0.0784 | 0.0648 | 0.0349 | 0.09 | 0.0781 | 0.0332 | 0.0998 | 0.0569 | 0.06 |
Golgi | 0.0076 | 0.0036 | 0.0076 | 0.0142 | 0.0088 | 0.003 | 0.0205 | 0.0033 | 0.0066 | 0.0093 | 0.0169 | 0.0092 | 0.0072 | 0.0086 | 0.0042 | 0.0052 | 0.0075 | 0.0067 |
Lipid Particles | 0.0035 | 0.001 | 0.0009 | 0.0004 | 0.001 | 0.0003 | 0.0213 | 0.0002 | 0.0027 | 0.0105 | 0.0587 | 0.0007 | 0.0124 | 0.0043 | 0.0008 | 0.0002 | 0.0291 | 0.0002 |
Mitochondria | 0.0104 | 0.0006 | 0.0014 | 0.0018 | 0.0012 | 0.0016 | 0.0029 | 0.0008 | 0.004 | 0.0169 | 0.002 | 0.0056 | 0.0127 | 0.0007 | 0.0005 | 0.0009 | 0.0011 | 0.001 |
None | 0.0053 | 0.0021 | 0.0025 | 0.0016 | 0.0009 | 0.002 | 0.0119 | 0.0034 | 0.008 | 0.0078 | 0.0035 | 0.0051 | 0.0111 | 0.0022 | 0.009 | 0.0026 | 0.0024 | 0.0419 |
Nuclear Periphery | 0.0037 | 0.0028 | 0.0043 | 0.0018 | 0.0025 | 0.0056 | 0.0125 | 0.0013 | 0.0023 | 0.0022 | 0.0014 | 0.0011 | 0.0022 | 0.0004 | 0.0023 | 0.0008 | 0.0004 | 0.0007 |
Nucleolus | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0008 | 0 | 0.0003 | 0.0009 | 0.0001 | 0.0001 | 0.0007 | 0 | 0 | 0 | 0.0001 | 0 |
Nucleus | 0.0046 | 0.0071 | 0.0105 | 0.0041 | 0.0078 | 0.0134 | 0.0239 | 0.0014 | 0.0028 | 0.003 | 0.0028 | 0.0033 | 0.0023 | 0.0007 | 0.0024 | 0.001 | 0.0006 | 0.0018 |
Peroxisomes | 0.0095 | 0.0012 | 0.0095 | 0.01 | 0.0016 | 0.0059 | 0.0209 | 0.0033 | 0.0171 | 0.0111 | 0.0128 | 0.0078 | 0.0042 | 0.0013 | 0.0226 | 0.0027 | 0.0684 | 0.0032 |
Punctate Nuclear | 0.0019 | 0.0015 | 0.0015 | 0.0013 | 0.001 | 0.0018 | 0.009 | 0.0007 | 0.003 | 0.0008 | 0.003 | 0.0046 | 0.0021 | 0.0006 | 0.0015 | 0.0008 | 0.0028 | 0.001 |
Vacuole | 0.0046 | 0.0029 | 0.003 | 0.0031 | 0.0021 | 0.0023 | 0.0026 | 0.0006 | 0.0016 | 0.0114 | 0.0012 | 0.0011 | 0.0069 | 0.0013 | 0.0011 | 0.0019 | 0.0023 | 0.0009 |
Vacuole Periphery | 0.0038 | 0.0003 | 0.0007 | 0.0008 | 0.0006 | 0.0006 | 0.0009 | 0.0002 | 0.0005 | 0.0119 | 0.0005 | 0.0004 | 0.0032 | 0.0003 | 0.0004 | 0.0007 | 0.0004 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 18.8233 | 11.485 | 16.0538 | 29.3125 | 11.4279 | 24.4866 | 22.8874 | 26.885 | 33.5858 | 26.4482 |
Translational Efficiency | 0.932 | 0.9767 | 0.9151 | 0.6966 | 1.0269 | 0.8924 | 0.8299 | 0.5966 | 0.6378 | 0.6188 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1531 | 17 | 227 | 1557 | 1167 | 1707 | 197 | 249 | 2698 | 1724 | 424 | 1806 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 862.85 | 1185.80 | 1541.07 | 1202.64 | 755.82 | 938.97 | 1354.90 | 1124.35 | 816.55 | 941.40 | 1454.57 | 1191.85 |
Standard Deviation | 139.03 | 150.25 | 188.23 | 169.23 | 126.90 | 129.29 | 146.87 | 148.41 | 144.03 | 131.79 | 193.94 | 168.69 |
Intensity Change Log 2 | — | 0.458679 | 0.836751 | 0.479023 | — | 0.313036 | 0.842072 | 0.572977 | — | 0.392498 | 0.839238 | 0.523656 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001896 | 0.002108 | 0.023495 | 0.009096 | 0.001630 | 0.001825 | 0.012974 | 0.001882 | 0.001781 | 0.001827 | 0.018607 | 0.008101 |
Bud Neck | 0.006379 | 0.002561 | 0.008564 | 0.009089 | 0.010428 | 0.008359 | 0.009485 | 0.007249 | 0.008130 | 0.008302 | 0.008992 | 0.008835 |
Bud Site | 0.023745 | 0.018253 | 0.071013 | 0.053007 | 0.020911 | 0.019138 | 0.073162 | 0.024567 | 0.022519 | 0.019129 | 0.072011 | 0.049086 |
Cell Periphery | 0.000122 | 0.000102 | 0.000368 | 0.000168 | 0.000122 | 0.000075 | 0.000229 | 0.000089 | 0.000122 | 0.000075 | 0.000303 | 0.000157 |
Cytoplasm | 0.149391* | 0.131500* | 0.144015* | 0.292156* | 0.204416* | 0.257071* | 0.109239* | 0.188082* | 0.173192* | 0.255833* | 0.127857* | 0.277807* |
Cytoplasmic Foci | 0.515598* | 0.608512* | 0.223494* | 0.155593 | 0.459956* | 0.428937* | 0.197343* | 0.154957 | 0.491530* | 0.430707* | 0.211344* | 0.155505 |
Eisosomes | 0.000090 | 0.000067 | 0.000046 | 0.000056 | 0.000138 | 0.000078 | 0.000074 | 0.000056 | 0.000110 | 0.000078 | 0.000059 | 0.000056 |
Endoplasmic Reticulum | 0.001763 | 0.000881 | 0.003341 | 0.002107 | 0.003161 | 0.001621 | 0.002821 | 0.001984 | 0.002368 | 0.001614 | 0.003099 | 0.002090 |
Endosome | 0.086261 | 0.067371 | 0.084257 | 0.048925 | 0.102383 | 0.086446 | 0.099008 | 0.077758 | 0.093235 | 0.086257 | 0.091111 | 0.052900 |
Golgi | 0.105309 | 0.016155 | 0.042051 | 0.009330 | 0.050892 | 0.029246 | 0.013232 | 0.002224 | 0.081771 | 0.029117 | 0.028661 | 0.008350 |
Lipid Particles | 0.010180 | 0.005432 | 0.027545 | 0.009692 | 0.018222 | 0.008677 | 0.023067 | 0.009654 | 0.013659 | 0.008645 | 0.025465 | 0.009687 |
Mitochondria | 0.009256 | 0.000194 | 0.000087 | 0.003498 | 0.012206 | 0.004392 | 0.000082 | 0.000195 | 0.010532 | 0.004350 | 0.000085 | 0.003043 |
Mitotic Spindle | 0.022237 | 0.035140 | 0.209939* | 0.170514* | 0.012756 | 0.022413 | 0.211035* | 0.132118* | 0.018136 | 0.022538 | 0.210448* | 0.165220* |
None | 0.001899 | 0.000214 | 0.000161 | 0.000773 | 0.004144 | 0.001612 | 0.000206 | 0.001559 | 0.002870 | 0.001598 | 0.000182 | 0.000881 |
Nuclear Periphery | 0.000107 | 0.000106 | 0.000618 | 0.000853 | 0.000614 | 0.000262 | 0.000730 | 0.001014 | 0.000326 | 0.000261 | 0.000670 | 0.000876 |
Nuclear Periphery Foci | 0.002315 | 0.001812 | 0.020681 | 0.005288 | 0.003163 | 0.001214 | 0.018785 | 0.012111 | 0.002682 | 0.001220 | 0.019800 | 0.006229 |
Nucleolus | 0.002063 | 0.000164 | 0.000120 | 0.000547 | 0.001703 | 0.000772 | 0.000188 | 0.000613 | 0.001908 | 0.000766 | 0.000152 | 0.000556 |
Nucleus | 0.005831 | 0.003535 | 0.010495 | 0.019465 | 0.013908 | 0.010539 | 0.011570 | 0.021819 | 0.009325 | 0.010470 | 0.010995 | 0.019790 |
Peroxisomes | 0.025090 | 0.034423 | 0.010015 | 0.019170 | 0.021615 | 0.025138 | 0.035867 | 0.019888 | 0.023587 | 0.025229 | 0.022027 | 0.019269 |
Vacuole | 0.025874 | 0.070860 | 0.117241 | 0.182840 | 0.052834 | 0.088077 | 0.179883 | 0.341337* | 0.037536 | 0.087907 | 0.146346 | 0.204693* |
Vacuole Periphery | 0.004593 | 0.000610 | 0.002454 | 0.007833 | 0.004798 | 0.004109 | 0.001021 | 0.000844 | 0.004682 | 0.004075 | 0.001788 | 0.006869 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.19 | -3.53 | -3.03 | -1.64 | 3.11 | -0.33 | -3.22 | 0.55 | 0.74 | 3.30 | -0.01 | -4.51 | -3.16 | -2.70 | 3.84 |
Bud Neck | 3.87 | -4.35 | -4.52 | -6.95 | 1.54 | 1.61 | -0.68 | 2.55 | 1.57 | 3.26 | -0.57 | -3.36 | -1.23 | -0.61 | 2.77 |
Bud Site | 0.57 | -7.57 | -2.74 | -1.66 | 6.46 | 0.93 | -5.82 | 0.24 | -0.43 | 5.72 | 2.16 | -9.39 | -3.27 | -5.17 | 7.89 |
Cell Periphery | 0.16 | -7.40 | 0.28 | -0.11 | 7.71 | 6.48 | -6.25 | 3.96 | -1.03 | 7.99 | 6.09 | -9.20 | 0.93 | -5.75 | 9.69 |
Cytoplasm | -0.37 | -1.65 | -18.40 | -2.58 | -7.76 | -5.54 | 4.49 | 0.82 | 4.22 | -2.76 | -10.56 | 1.26 | -15.85 | -4.12 | -11.13 |
Cytoplasmic Foci | -0.88 | 25.09 | 63.48 | 8.46 | 7.94 | 3.84 | 22.37 | 33.41 | 31.64 | 4.41 | 8.81 | 33.73 | 72.81 | 48.79 | 9.35 |
Eisosomes | 0.59 | 6.12 | 8.64 | 0.65 | 0.95 | 7.06 | 6.25 | 9.64 | 4.40 | 2.83 | 5.98 | 8.50 | 13.27 | 7.54 | 3.41 |
Endoplasmic Reticulum | 1.36 | -2.36 | 2.18 | -0.56 | 3.19 | 2.76 | -0.58 | 2.19 | -0.87 | 3.55 | 2.31 | -2.35 | 3.79 | 1.09 | 5.09 |
Endosome | 1.69 | 2.59 | 19.08 | 2.60 | 7.58 | 3.46 | 4.07 | 8.87 | 6.99 | 2.94 | 2.20 | 4.62 | 24.17 | 19.81 | 9.58 |
Golgi | 14.93 | 12.22 | 27.96 | 2.27 | 5.85 | 7.20 | 13.51 | 18.61 | 16.54 | 4.08 | 18.40 | 16.64 | 32.35 | 15.58 | 6.45 |
Lipid Particles | 1.92 | -5.13 | 1.18 | -1.42 | 5.48 | 6.68 | -1.28 | 6.40 | -0.07 | 5.27 | 4.99 | -5.20 | 5.41 | -0.53 | 7.23 |
Mitochondria | 8.16 | 8.24 | 6.96 | -3.01 | -3.29 | 3.79 | 6.22 | 6.19 | 5.51 | -1.68 | 4.88 | 9.91 | 8.70 | 4.05 | -3.30 |
Mitotic Spindle | -1.37 | -9.70 | -15.46 | -3.77 | 3.77 | -3.55 | -8.85 | -7.00 | -6.31 | 2.73 | -2.13 | -13.09 | -17.67 | -15.90 | 4.95 |
None | 2.65 | 2.88 | 0.86 | -4.86 | -7.86 | 1.85 | 3.25 | 2.37 | 0.47 | -2.10 | 1.40 | 4.29 | 2.42 | 0.25 | -7.80 |
Nuclear Periphery | -0.42 | -6.84 | -21.20 | -18.70 | -6.21 | 1.30 | -2.21 | -3.97 | -8.76 | -2.63 | 0.35 | -5.10 | -10.58 | -16.69 | -5.91 |
Nuclear Periphery Foci | 0.20 | -7.87 | -6.26 | -2.95 | 6.63 | 3.02 | -7.48 | -4.79 | -5.68 | 1.13 | 3.90 | -10.81 | -7.22 | -11.50 | 8.18 |
Nucleolus | 3.46 | 3.53 | 2.42 | -8.50 | -10.23 | 2.89 | 4.57 | 2.69 | -0.32 | -4.06 | 3.32 | 5.03 | 3.35 | -0.29 | -10.44 |
Nucleus | 1.22 | -3.30 | -17.46 | -14.69 | -5.28 | 2.04 | -1.32 | -3.18 | -4.28 | -1.36 | -1.30 | -3.42 | -15.71 | -15.00 | -6.15 |
Peroxisomes | -0.67 | 7.63 | 9.07 | 2.13 | -0.45 | -1.75 | -0.76 | 3.67 | 4.71 | 2.87 | -0.89 | 2.95 | 10.65 | 9.14 | 2.84 |
Vacuole | -1.99 | -9.57 | -43.83 | -10.89 | -11.83 | -9.51 | -11.59 | -20.10 | -17.95 | -7.65 | -15.53 | -14.79 | -46.56 | -35.34 | -12.07 |
Vacuole Periphery | 9.28 | 5.01 | 3.97 | -2.45 | -0.03 | 1.41 | 9.03 | 9.89 | 9.05 | 2.54 | 1.56 | 9.37 | 5.29 | 3.59 | -0.69 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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