Standard name | |
---|---|
Human Ortholog | |
Description | Karyopherin/importin that interacts with the nuclear pore complex; acts as the nuclear import receptor for specific proteins, including Pdr1p, Yap1p, Ste12p, and Aft1p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 3 | 4 | 5 | 4 | 9 | 4 | 7 | 4 | 5 | 1 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud | 0 | 0 | 3 | 2 | 0 | 6 | 4 | 9 | 7 | 8 | 4 | 10 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 3 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 2 | 1 | 2 | 0 | 1 | 4 | 2 | 3 | 4 | 4 | 0 | 0 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 115 | 108 | 213 | 96 | 166 | 124 | 201 | 191 | 112 | 151 | 87 | 118 | 109 | 155 | 146 | 106 | 54 | 102 | 100 | 97 | 251 | 48 | 168 | 152 |
Endoplasmic Reticulum | 5 | 2 | 17 | 26 | 51 | 30 | 30 | 49 | 16 | 32 | 33 | 29 | 18 | 36 | 20 | 3 | 2 | 2 | 9 | 3 | 35 | 4 | 18 | 27 |
Endosome | 0 | 0 | 1 | 35 | 67 | 37 | 42 | 37 | 20 | 23 | 13 | 18 | 5 | 19 | 41 | 0 | 2 | 0 | 0 | 1 | 7 | 2 | 4 | 4 |
Golgi | 0 | 0 | 0 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 2 | 3 | 2 | 31 | 34 | 61 | 65 | 109 | 71 | 158 | 1 | 0 | 2 | 1 | 0 | 1 | 0 | 1 | 2 | 0 | 0 | 0 |
Nucleus | 1 | 0 | 0 | 1 | 0 | 0 | 4 | 4 | 2 | 1 | 4 | 3 | 1 | 0 | 1 | 1 | 1 | 5 | 7 | 8 | 14 | 4 | 7 | 2 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 3 | 3 | 5 | 5 | 3 | 0 | 5 | 8 | 1 | 0 | 0 | 0 | 0 | 0 | 17 | 7 | 20 | 3 | 8 | 11 |
Nucleolus | 0 | 0 | 2 | 16 | 12 | 5 | 8 | 12 | 3 | 3 | 1 | 7 | 4 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 3 | 2 | 1 | 3 | 4 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 0 | 0 | 1 |
Vac/Vac Membrane | 20 | 5 | 69 | 92 | 86 | 26 | 96 | 120 | 45 | 48 | 35 | 66 | 15 | 10 | 17 | 19 | 18 | 14 | 7 | 16 | 36 | 6 | 14 | 17 |
Unique Cell Count | 135 | 113 | 297 | 240 | 345 | 239 | 395 | 439 | 241 | 334 | 224 | 367 | 146 | 203 | 222 | 123 | 69 | 121 | 146 | 140 | 383 | 75 | 234 | 226 |
Labelled Cell Count | 141 | 115 | 309 | 280 | 399 | 268 | 432 | 505 | 281 | 386 | 261 | 426 | 158 | 222 | 239 | 132 | 80 | 125 | 146 | 140 | 383 | 75 | 234 | 226 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 23.0 | 17.3 | 12.2 | 11.6 | 11.1 | 9.1 | 8.6 | 8.2 | 7.8 | 7.6 | 7.4 | 7.2 | 18.9 | 17.3 | 14.7 | 18.1 | 20.9 | 20.2 | 16.2 | 20.3 | 21.8 |
Std Deviation (1e-4) | 3.8 | 3.2 | 2.4 | 1.8 | 1.6 | 1.4 | 1.7 | 1.3 | 1.5 | 1.5 | 1.4 | 1.3 | 4.6 | 3.0 | 2.7 | 4.4 | 4.1 | 4.9 | 5.4 | 4.2 | 4.3 |
Intensity Change (Log2) | – | – | – | -0.07 | -0.13 | -0.41 | -0.49 | -0.58 | -0.64 | -0.69 | -0.72 | -0.77 | 0.64 | 0.51 | 0.27 | 0.57 | 0.78 | 0.73 | 0.41 | 0.74 | 0.84 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 1.3 | 0 | 1.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -7.4 | -6.1 | -4.7 | -5.5 | -7.5 | -6.0 | -6.7 | -7.5 | -10.1 | 0.7 | 1.2 | -1.5 | 3.2 | 1.1 | 2.7 |
Endoplasmic Reticulum | 2.2 | 3.7 | 2.8 | 1.0 | 2.5 | 0.4 | 1.8 | 3.5 | 1.1 | 2.4 | 4.3 | 1.4 | -1.4 | 0 | -1.8 |
Endosome | 6.6 | 7.8 | 6.8 | 5.6 | 4.9 | 4.7 | 4.3 | 3.8 | 3.5 | 0 | 5.1 | 7.5 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 5.9 | 4.7 | 6.3 | 9.2 | 10.5 | 10.1 | 12.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 3.8 | 2.4 | 0 | 0 | 2.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 3.8 | 0.5 | -3.7 | 0.3 | 1.2 | -1.3 | -2.9 | -2.2 | -1.7 | -3.3 | -5.5 | -4.7 | -1.8 | 0.5 | -2.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 14.416 | 20.9874 | 17.2655 | 16.2258 | 14.5325 | 16.8432 | 13.4931 | 18.5162 | 16.7336 | 15.2569 | 14.8226 | 17.4307 | 18.4004 | 22.1027 | 20.044 | 19.016 | 19.004 | 20.1566 |
Actin | 0.0253 | 0.0002 | 0.0068 | 0.0002 | 0.0256 | 0.0038 | 0.0274 | 0.0002 | 0.0053 | 0.0437 | 0.0021 | 0.0002 | 0.0061 | 0.0001 | 0.0096 | 0.0003 | 0.0078 | 0.0002 |
Bud | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0017 | 0.0002 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0.0001 | 0 |
Bud Neck | 0.0003 | 0.0002 | 0.0006 | 0.0003 | 0.0009 | 0.0018 | 0.0004 | 0.0002 | 0.0004 | 0.0006 | 0.0007 | 0.0007 | 0.0002 | 0.0001 | 0.0018 | 0.0004 | 0.0003 | 0.0005 |
Bud Periphery | 0.0002 | 0 | 0.0002 | 0 | 0.0008 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0024 | 0.0002 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0.0001 | 0 |
Bud Site | 0.0004 | 0.0001 | 0.0008 | 0.0001 | 0.0005 | 0.0007 | 0.0006 | 0.0004 | 0.0003 | 0.0058 | 0.0005 | 0 | 0.0005 | 0 | 0.0058 | 0.0001 | 0.0003 | 0 |
Cell Periphery | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.2772 | 0.4118 | 0.3331 | 0.4483 | 0.2269 | 0.1835 | 0.4063 | 0.5344 | 0.5221 | 0.5383 | 0.3828 | 0.4667 | 0.3314 | 0.4237 | 0.4324 | 0.4721 | 0.5627 | 0.3147 |
Cytoplasmic Foci | 0.0048 | 0.0008 | 0.0123 | 0.0009 | 0.0046 | 0.0023 | 0.0102 | 0.015 | 0.0121 | 0.0021 | 0.0092 | 0.0016 | 0.0142 | 0.0212 | 0.011 | 0.0085 | 0.0092 | 0.0054 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0 | 0.0005 | 0.0001 | 0.0002 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0222 | 0.01 | 0.0153 | 0.014 | 0.0168 | 0.0133 | 0.0459 | 0.0251 | 0.0245 | 0.0362 | 0.0297 | 0.021 | 0.0415 | 0.022 | 0.0312 | 0.0227 | 0.014 | 0.0256 |
Endosome | 0.0077 | 0.0041 | 0.0139 | 0.0064 | 0.0235 | 0.0141 | 0.0297 | 0.0102 | 0.0123 | 0.0094 | 0.056 | 0.0044 | 0.0352 | 0.0054 | 0.0087 | 0.0255 | 0.0164 | 0.0194 |
Golgi | 0.0032 | 0.0001 | 0.0038 | 0.0001 | 0.0204 | 0.0013 | 0.004 | 0.0005 | 0.0027 | 0.0079 | 0.0044 | 0 | 0.0066 | 0.0001 | 0.0038 | 0.0003 | 0.0058 | 0.0004 |
Lipid Particles | 0.0096 | 0.0001 | 0.0081 | 0.0001 | 0.0381 | 0.0014 | 0.0111 | 0.0002 | 0.0027 | 0.0021 | 0.0042 | 0 | 0.0046 | 0.0002 | 0.0052 | 0.0014 | 0.0042 | 0.0006 |
Mitochondria | 0.018 | 0.0002 | 0.003 | 0.0004 | 0.0386 | 0.0006 | 0.0018 | 0.0002 | 0.0007 | 0.0105 | 0.0035 | 0.0002 | 0.0018 | 0.0001 | 0.0011 | 0.0001 | 0.0038 | 0.0002 |
None | 0.0013 | 0.0002 | 0.0008 | 0.0004 | 0.0005 | 0.0007 | 0.0067 | 0.0004 | 0.0009 | 0.0005 | 0.0023 | 0.0004 | 0.0011 | 0.0002 | 0.0005 | 0.0009 | 0.0005 | 0.0007 |
Nuclear Periphery | 0.4341 | 0.3318 | 0.3803 | 0.3564 | 0.3163 | 0.4331 | 0.2965 | 0.2157 | 0.2553 | 0.177 | 0.2678 | 0.2721 | 0.3418 | 0.3375 | 0.326 | 0.2978 | 0.2109 | 0.3444 |
Nucleolus | 0.0007 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0005 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0011 | 0 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 |
Nucleus | 0.1775 | 0.2313 | 0.2052 | 0.1578 | 0.2719 | 0.3281 | 0.1467 | 0.1808 | 0.1458 | 0.1548 | 0.2113 | 0.2263 | 0.1961 | 0.1747 | 0.1487 | 0.1568 | 0.1433 | 0.2773 |
Peroxisomes | 0.0007 | 0 | 0.0049 | 0 | 0.0029 | 0.0001 | 0.0017 | 0.0001 | 0.0042 | 0.0008 | 0.0022 | 0 | 0.0016 | 0 | 0.0076 | 0 | 0.0119 | 0.0001 |
Punctate Nuclear | 0.0038 | 0.001 | 0.0034 | 0.001 | 0.0029 | 0.0022 | 0.0034 | 0.0111 | 0.0057 | 0.0015 | 0.0128 | 0.0023 | 0.0033 | 0.0096 | 0.0025 | 0.0039 | 0.0026 | 0.0043 |
Vacuole | 0.0064 | 0.0046 | 0.0038 | 0.0079 | 0.0038 | 0.0074 | 0.0044 | 0.0032 | 0.003 | 0.0026 | 0.0047 | 0.0024 | 0.0085 | 0.0028 | 0.0016 | 0.0074 | 0.0035 | 0.0035 |
Vacuole Periphery | 0.006 | 0.0034 | 0.0031 | 0.0055 | 0.0036 | 0.0045 | 0.0024 | 0.0014 | 0.0016 | 0.0014 | 0.0045 | 0.0017 | 0.0048 | 0.0021 | 0.0015 | 0.0015 | 0.0025 | 0.0025 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 134.6356 | 112.5132 | 95.3528 | 115.6379 | 112.874 | 97.1268 | 92.7938 | 85.5303 | 83.8122 | 119.1189 |
Translational Efficiency | 0.7538 | 0.7897 | 0.8101 | 0.6465 | 0.692 | 0.8566 | 0.6993 | 0.5914 | 0.7057 | 0.6171 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1692 | 1596 | 447 | 953 | 1392 | 1739 | 860 | 533 | 3084 | 3335 | 1307 | 1486 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1125.76 | 1598.68 | 2155.12 | 1700.98 | 1251.07 | 1562.94 | 1748.13 | 1902.82 | 1182.32 | 1580.04 | 1887.32 | 1773.38 |
Standard Deviation | 193.96 | 309.73 | 304.81 | 318.78 | 237.20 | 275.48 | 367.35 | 332.11 | 223.44 | 292.92 | 397.30 | 337.79 |
Intensity Change Log 2 | — | 0.505982 | 0.936869 | 0.595467 | — | 0.321100 | 0.482650 | 0.604977 | — | 0.411625 | 0.715637 | 0.600480 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000134 | 0.000097 | 0.000236 | 0.000814 | 0.000215 | 0.000261 | 0.000332 | 0.000310 | 0.000171 | 0.000182 | 0.000299 | 0.000633 |
Bud Neck | 0.001414 | 0.001682 | 0.013120 | 0.007387 | 0.001787 | 0.001988 | 0.005961 | 0.007721 | 0.001582 | 0.001841 | 0.008409 | 0.007507 |
Bud Site | 0.000850 | 0.001007 | 0.001091 | 0.016568 | 0.001401 | 0.001529 | 0.002075 | 0.012605 | 0.001099 | 0.001279 | 0.001738 | 0.015147 |
Cell Periphery | 0.000052 | 0.000056 | 0.000354 | 0.000218 | 0.000069 | 0.000059 | 0.000169 | 0.000292 | 0.000059 | 0.000057 | 0.000232 | 0.000245 |
Cytoplasm | 0.634875* | 0.546348* | 0.320604* | 0.365515* | 0.564192* | 0.501155* | 0.351555* | 0.328624* | 0.602971* | 0.522783* | 0.340969* | 0.352283* |
Cytoplasmic Foci | 0.011660 | 0.006674 | 0.003180 | 0.018187 | 0.006327 | 0.011547 | 0.003215 | 0.010129 | 0.009253 | 0.009215 | 0.003203 | 0.015297 |
Eisosomes | 0.000003 | 0.000002 | 0.000003 | 0.000009 | 0.000003 | 0.000003 | 0.000011 | 0.000005 | 0.000003 | 0.000002 | 0.000009 | 0.000007 |
Endoplasmic Reticulum | 0.015192 | 0.016141 | 0.003635 | 0.004921 | 0.012295 | 0.020511 | 0.009652 | 0.002284 | 0.013885 | 0.018420 | 0.007594 | 0.003975 |
Endosome | 0.003699 | 0.004560 | 0.005066 | 0.023936 | 0.003851 | 0.006579 | 0.005619 | 0.008196 | 0.003767 | 0.005613 | 0.005430 | 0.018290 |
Golgi | 0.000414 | 0.001060 | 0.000807 | 0.007188 | 0.000697 | 0.001560 | 0.000610 | 0.001195 | 0.000541 | 0.001321 | 0.000678 | 0.005038 |
Lipid Particles | 0.002421 | 0.002538 | 0.005371 | 0.011216 | 0.002201 | 0.003373 | 0.003840 | 0.019691 | 0.002322 | 0.002973 | 0.004364 | 0.014256 |
Mitochondria | 0.000603 | 0.000596 | 0.001038 | 0.005003 | 0.000593 | 0.000705 | 0.000884 | 0.001579 | 0.000599 | 0.000653 | 0.000937 | 0.003775 |
Mitotic Spindle | 0.021109 | 0.036647 | 0.097108 | 0.254077* | 0.033042 | 0.043805 | 0.062739 | 0.208801* | 0.026495 | 0.040380 | 0.074493 | 0.237837* |
None | 0.008924 | 0.003386 | 0.004350 | 0.010687 | 0.006467 | 0.002491 | 0.005812 | 0.005070 | 0.007815 | 0.002919 | 0.005312 | 0.008672 |
Nuclear Periphery | 0.035608 | 0.066847 | 0.106577* | 0.021704 | 0.044728 | 0.089479 | 0.102228* | 0.036514 | 0.039725 | 0.078648 | 0.103716* | 0.027016 |
Nuclear Periphery Foci | 0.138679 | 0.230518* | 0.211023* | 0.094225 | 0.218473* | 0.221535* | 0.148200 | 0.192553* | 0.174695 | 0.225834* | 0.169686 | 0.129493 |
Nucleolus | 0.001537 | 0.000492 | 0.000550 | 0.000853 | 0.001307 | 0.000733 | 0.001190 | 0.000385 | 0.001434 | 0.000618 | 0.000971 | 0.000685 |
Nucleus | 0.103144 | 0.064577 | 0.194423* | 0.089621 | 0.085468 | 0.067078 | 0.236350* | 0.108901 | 0.095166 | 0.065881 | 0.222011* | 0.096537 |
Peroxisomes | 0.000072 | 0.000025 | 0.000034 | 0.001158 | 0.000097 | 0.000079 | 0.000093 | 0.000278 | 0.000083 | 0.000053 | 0.000073 | 0.000842 |
Vacuole | 0.018836 | 0.015823 | 0.030912 | 0.062446 | 0.015632 | 0.023807 | 0.058642 | 0.051618 | 0.017390 | 0.019986 | 0.049158 | 0.058562 |
Vacuole Periphery | 0.000774 | 0.000924 | 0.000517 | 0.004268 | 0.001157 | 0.001724 | 0.000824 | 0.003247 | 0.000947 | 0.001341 | 0.000719 | 0.003902 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.60 | -7.23 | -3.89 | -4.35 | 0.07 | -0.40 | -4.59 | -1.47 | -0.13 | 2.92 | -0.26 | -8.24 | -3.99 | -2.43 | 0.88 |
Bud Neck | -1.23 | -11.19 | -12.08 | -12.01 | 6.17 | -1.72 | -9.02 | -9.39 | -8.86 | -1.87 | -2.15 | -14.08 | -15.35 | -14.77 | 2.93 |
Bud Site | -1.24 | -6.01 | -7.00 | -6.16 | -5.23 | -0.29 | -2.37 | -3.77 | -3.77 | -3.07 | -1.16 | -5.11 | -7.61 | -7.17 | -5.88 |
Cell Periphery | -2.06 | -10.47 | -8.77 | -8.15 | 8.01 | 1.15 | -9.35 | -6.76 | -6.98 | -2.77 | -0.44 | -13.37 | -10.37 | -10.28 | 4.77 |
Cytoplasm | 8.08 | 25.67 | 23.21 | 14.68 | -5.55 | 5.08 | 18.69 | 15.11 | 11.67 | -0.35 | 9.79 | 31.97 | 26.82 | 18.33 | -4.03 |
Cytoplasmic Foci | 5.32 | 7.20 | 0.80 | -2.70 | -3.85 | -4.16 | 3.60 | -2.74 | 0.04 | -5.07 | 0.22 | 8.72 | -1.27 | -1.45 | -6.66 |
Eisosomes | 5.62 | -5.66 | -9.10 | -13.78 | -1.97 | -0.62 | -10.68 | -6.10 | -5.41 | 6.18 | 1.88 | -12.00 | -10.88 | -12.39 | 4.78 |
Endoplasmic Reticulum | -0.46 | 10.55 | 11.40 | 11.89 | 2.86 | -4.89 | 3.82 | 10.27 | 15.78 | 8.96 | -3.81 | 8.79 | 14.89 | 18.92 | 7.97 |
Endosome | -2.22 | -5.44 | -4.45 | -3.61 | -1.70 | -5.74 | -5.07 | -3.03 | 0.97 | 0.43 | -6.00 | -7.41 | -5.19 | -2.60 | -1.65 |
Golgi | -1.53 | -6.06 | -2.68 | -1.18 | -1.33 | -2.05 | 1.03 | 0.12 | 2.14 | -0.99 | -2.62 | -1.10 | -2.52 | -0.29 | -1.99 |
Lipid Particles | -1.42 | -7.58 | -8.02 | -7.65 | -0.92 | -4.01 | -7.17 | -9.15 | -8.51 | -7.45 | -4.06 | -10.38 | -12.08 | -11.21 | -7.07 |
Mitochondria | 0.06 | -5.48 | -1.55 | -1.49 | -0.09 | -0.60 | -3.73 | -0.60 | -0.24 | 1.15 | -0.32 | -6.69 | -1.65 | -1.49 | -0.13 |
Mitotic Spindle | -4.56 | -8.72 | -17.60 | -15.25 | -7.09 | -2.43 | -6.97 | -11.80 | -10.90 | -7.29 | -5.09 | -11.34 | -20.98 | -18.70 | -11.00 |
None | 5.44 | 3.95 | -0.62 | -6.39 | -4.81 | 6.02 | 1.53 | -0.37 | -5.80 | -1.80 | 7.79 | 3.83 | -0.98 | -8.41 | -4.72 |
Nuclear Periphery | -7.88 | -8.44 | 6.15 | 13.39 | 11.25 | -9.32 | -5.85 | 5.16 | 13.50 | 9.24 | -12.48 | -10.02 | 8.40 | 19.83 | 14.73 |
Nuclear Periphery Foci | -9.73 | -3.13 | 12.75 | 21.71* | 11.08 | 0.35 | 9.64 | 8.02 | 8.46 | -0.54 | -6.78 | 4.90 | 15.43 | 22.86* | 8.63 |
Nucleolus | 4.18 | 1.56 | 0.44 | -5.88 | -1.48 | 2.43 | 1.21 | 2.41 | -0.02 | 1.54 | 4.69 | 2.13 | 1.37 | -4.75 | -1.12 |
Nucleus | 8.91 | -10.36 | -4.93 | -11.24 | 7.22 | 3.29 | -18.16 | -4.83 | -6.73 | 10.68 | 8.76 | -20.44 | -7.22 | -12.94 | 13.45 |
Peroxisomes | 2.36 | 0.23 | -2.73 | -3.12 | -2.78 | 0.42 | -0.44 | -1.21 | -1.48 | -1.07 | 1.35 | -0.66 | -2.94 | -3.32 | -2.80 |
Vacuole | 1.52 | -7.65 | -20.06 | -20.54 | -12.37 | -5.43 | -15.76 | -14.18 | -12.65 | -2.89 | -3.00 | -17.13 | -24.55 | -23.73 | -9.93 |
Vacuole Periphery | -0.39 | 1.62 | -1.34 | -0.73 | -2.12 | -1.04 | 1.85 | 0.34 | 1.00 | -0.65 | -1.21 | 1.91 | -0.54 | 0.50 | -1.70 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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