Standard name | |
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Human Ortholog | |
Description | Karyopherin/importin that interacts with the nuclear pore complex; acts as the nuclear import receptor for specific proteins, including Pdr1p, Yap1p, Ste12p, and Aft1p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 3 | 4 | 5 | 4 | 9 | 4 | 7 | 4 | 5 | 1 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud | 0 | 0 | 3 | 2 | 0 | 6 | 4 | 9 | 7 | 8 | 4 | 10 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 3 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 2 | 1 | 2 | 0 | 1 | 4 | 2 | 3 | 4 | 4 | 0 | 0 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 115 | 108 | 213 | 96 | 166 | 124 | 201 | 191 | 112 | 151 | 87 | 118 | 109 | 155 | 146 | 106 | 54 | 102 | 100 | 97 | 251 | 48 | 168 | 152 |
Endoplasmic Reticulum | 5 | 2 | 17 | 26 | 51 | 30 | 30 | 49 | 16 | 32 | 33 | 29 | 18 | 36 | 20 | 3 | 2 | 2 | 9 | 3 | 35 | 4 | 18 | 27 |
Endosome | 0 | 0 | 1 | 35 | 67 | 37 | 42 | 37 | 20 | 23 | 13 | 18 | 5 | 19 | 41 | 0 | 2 | 0 | 0 | 1 | 7 | 2 | 4 | 4 |
Golgi | 0 | 0 | 0 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 2 | 3 | 2 | 31 | 34 | 61 | 65 | 109 | 71 | 158 | 1 | 0 | 2 | 1 | 0 | 1 | 0 | 1 | 2 | 0 | 0 | 0 |
Nucleus | 1 | 0 | 0 | 1 | 0 | 0 | 4 | 4 | 2 | 1 | 4 | 3 | 1 | 0 | 1 | 1 | 1 | 5 | 7 | 8 | 14 | 4 | 7 | 2 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 3 | 3 | 5 | 5 | 3 | 0 | 5 | 8 | 1 | 0 | 0 | 0 | 0 | 0 | 17 | 7 | 20 | 3 | 8 | 11 |
Nucleolus | 0 | 0 | 2 | 16 | 12 | 5 | 8 | 12 | 3 | 3 | 1 | 7 | 4 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 3 | 2 | 1 | 3 | 4 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 0 | 0 | 1 |
Vac/Vac Membrane | 20 | 5 | 69 | 92 | 86 | 26 | 96 | 120 | 45 | 48 | 35 | 66 | 15 | 10 | 17 | 19 | 18 | 14 | 7 | 16 | 36 | 6 | 14 | 17 |
Unique Cell Count | 135 | 113 | 297 | 240 | 345 | 239 | 395 | 439 | 241 | 334 | 224 | 367 | 146 | 203 | 222 | 123 | 69 | 121 | 146 | 140 | 383 | 75 | 234 | 226 |
Labelled Cell Count | 141 | 115 | 309 | 280 | 399 | 268 | 432 | 505 | 281 | 386 | 261 | 426 | 158 | 222 | 239 | 132 | 80 | 125 | 146 | 140 | 383 | 75 | 234 | 226 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 23.0 | 17.3 | 12.2 | 11.6 | 11.1 | 9.1 | 8.6 | 8.2 | 7.8 | 7.6 | 7.4 | 7.2 | 18.9 | 17.3 | 14.7 | 18.1 | 20.9 | 20.2 | 16.2 | 20.3 | 21.8 |
Std Deviation (1e-4) | 3.8 | 3.2 | 2.4 | 1.8 | 1.6 | 1.4 | 1.7 | 1.3 | 1.5 | 1.5 | 1.4 | 1.3 | 4.6 | 3.0 | 2.7 | 4.4 | 4.1 | 4.9 | 5.4 | 4.2 | 4.3 |
Intensity Change (Log2) | – | – | – | -0.07 | -0.13 | -0.41 | -0.49 | -0.58 | -0.64 | -0.69 | -0.72 | -0.77 | 0.64 | 0.51 | 0.27 | 0.57 | 0.78 | 0.73 | 0.41 | 0.74 | 0.84 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 1.3 | 0 | 1.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -7.4 | -6.1 | -4.7 | -5.5 | -7.5 | -6.0 | -6.7 | -7.5 | -10.1 | 0.7 | 1.2 | -1.5 | 3.2 | 1.1 | 2.7 |
Endoplasmic Reticulum | 2.2 | 3.7 | 2.8 | 1.0 | 2.5 | 0.4 | 1.8 | 3.5 | 1.1 | 2.4 | 4.3 | 1.4 | -1.4 | 0 | -1.8 |
Endosome | 6.6 | 7.8 | 6.8 | 5.6 | 4.9 | 4.7 | 4.3 | 3.8 | 3.5 | 0 | 5.1 | 7.5 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 5.9 | 4.7 | 6.3 | 9.2 | 10.5 | 10.1 | 12.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 3.8 | 2.4 | 0 | 0 | 2.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 3.8 | 0.5 | -3.7 | 0.3 | 1.2 | -1.3 | -2.9 | -2.2 | -1.7 | -3.3 | -5.5 | -4.7 | -1.8 | 0.5 | -2.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 14.416 | 20.9874 | 17.2655 | 16.2258 | 14.5325 | 16.8432 | 13.4931 | 18.5162 | 16.7336 | 15.2569 | 14.8226 | 17.4307 | 18.4004 | 22.1027 | 20.044 | 19.016 | 19.004 | 20.1566 |
Actin | 0.0253 | 0.0002 | 0.0068 | 0.0002 | 0.0256 | 0.0038 | 0.0274 | 0.0002 | 0.0053 | 0.0437 | 0.0021 | 0.0002 | 0.0061 | 0.0001 | 0.0096 | 0.0003 | 0.0078 | 0.0002 |
Bud | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0017 | 0.0002 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0.0001 | 0 |
Bud Neck | 0.0003 | 0.0002 | 0.0006 | 0.0003 | 0.0009 | 0.0018 | 0.0004 | 0.0002 | 0.0004 | 0.0006 | 0.0007 | 0.0007 | 0.0002 | 0.0001 | 0.0018 | 0.0004 | 0.0003 | 0.0005 |
Bud Periphery | 0.0002 | 0 | 0.0002 | 0 | 0.0008 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0024 | 0.0002 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0.0001 | 0 |
Bud Site | 0.0004 | 0.0001 | 0.0008 | 0.0001 | 0.0005 | 0.0007 | 0.0006 | 0.0004 | 0.0003 | 0.0058 | 0.0005 | 0 | 0.0005 | 0 | 0.0058 | 0.0001 | 0.0003 | 0 |
Cell Periphery | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.2772 | 0.4118 | 0.3331 | 0.4483 | 0.2269 | 0.1835 | 0.4063 | 0.5344 | 0.5221 | 0.5383 | 0.3828 | 0.4667 | 0.3314 | 0.4237 | 0.4324 | 0.4721 | 0.5627 | 0.3147 |
Cytoplasmic Foci | 0.0048 | 0.0008 | 0.0123 | 0.0009 | 0.0046 | 0.0023 | 0.0102 | 0.015 | 0.0121 | 0.0021 | 0.0092 | 0.0016 | 0.0142 | 0.0212 | 0.011 | 0.0085 | 0.0092 | 0.0054 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0 | 0.0005 | 0.0001 | 0.0002 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0222 | 0.01 | 0.0153 | 0.014 | 0.0168 | 0.0133 | 0.0459 | 0.0251 | 0.0245 | 0.0362 | 0.0297 | 0.021 | 0.0415 | 0.022 | 0.0312 | 0.0227 | 0.014 | 0.0256 |
Endosome | 0.0077 | 0.0041 | 0.0139 | 0.0064 | 0.0235 | 0.0141 | 0.0297 | 0.0102 | 0.0123 | 0.0094 | 0.056 | 0.0044 | 0.0352 | 0.0054 | 0.0087 | 0.0255 | 0.0164 | 0.0194 |
Golgi | 0.0032 | 0.0001 | 0.0038 | 0.0001 | 0.0204 | 0.0013 | 0.004 | 0.0005 | 0.0027 | 0.0079 | 0.0044 | 0 | 0.0066 | 0.0001 | 0.0038 | 0.0003 | 0.0058 | 0.0004 |
Lipid Particles | 0.0096 | 0.0001 | 0.0081 | 0.0001 | 0.0381 | 0.0014 | 0.0111 | 0.0002 | 0.0027 | 0.0021 | 0.0042 | 0 | 0.0046 | 0.0002 | 0.0052 | 0.0014 | 0.0042 | 0.0006 |
Mitochondria | 0.018 | 0.0002 | 0.003 | 0.0004 | 0.0386 | 0.0006 | 0.0018 | 0.0002 | 0.0007 | 0.0105 | 0.0035 | 0.0002 | 0.0018 | 0.0001 | 0.0011 | 0.0001 | 0.0038 | 0.0002 |
None | 0.0013 | 0.0002 | 0.0008 | 0.0004 | 0.0005 | 0.0007 | 0.0067 | 0.0004 | 0.0009 | 0.0005 | 0.0023 | 0.0004 | 0.0011 | 0.0002 | 0.0005 | 0.0009 | 0.0005 | 0.0007 |
Nuclear Periphery | 0.4341 | 0.3318 | 0.3803 | 0.3564 | 0.3163 | 0.4331 | 0.2965 | 0.2157 | 0.2553 | 0.177 | 0.2678 | 0.2721 | 0.3418 | 0.3375 | 0.326 | 0.2978 | 0.2109 | 0.3444 |
Nucleolus | 0.0007 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0005 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0011 | 0 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 |
Nucleus | 0.1775 | 0.2313 | 0.2052 | 0.1578 | 0.2719 | 0.3281 | 0.1467 | 0.1808 | 0.1458 | 0.1548 | 0.2113 | 0.2263 | 0.1961 | 0.1747 | 0.1487 | 0.1568 | 0.1433 | 0.2773 |
Peroxisomes | 0.0007 | 0 | 0.0049 | 0 | 0.0029 | 0.0001 | 0.0017 | 0.0001 | 0.0042 | 0.0008 | 0.0022 | 0 | 0.0016 | 0 | 0.0076 | 0 | 0.0119 | 0.0001 |
Punctate Nuclear | 0.0038 | 0.001 | 0.0034 | 0.001 | 0.0029 | 0.0022 | 0.0034 | 0.0111 | 0.0057 | 0.0015 | 0.0128 | 0.0023 | 0.0033 | 0.0096 | 0.0025 | 0.0039 | 0.0026 | 0.0043 |
Vacuole | 0.0064 | 0.0046 | 0.0038 | 0.0079 | 0.0038 | 0.0074 | 0.0044 | 0.0032 | 0.003 | 0.0026 | 0.0047 | 0.0024 | 0.0085 | 0.0028 | 0.0016 | 0.0074 | 0.0035 | 0.0035 |
Vacuole Periphery | 0.006 | 0.0034 | 0.0031 | 0.0055 | 0.0036 | 0.0045 | 0.0024 | 0.0014 | 0.0016 | 0.0014 | 0.0045 | 0.0017 | 0.0048 | 0.0021 | 0.0015 | 0.0015 | 0.0025 | 0.0025 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 134.6356 | 112.5132 | 95.3528 | 115.6379 | 112.874 | 97.1268 | 92.7938 | 85.5303 | 83.8122 | 119.1189 |
Translational Efficiency | 0.7538 | 0.7897 | 0.8101 | 0.6465 | 0.692 | 0.8566 | 0.6993 | 0.5914 | 0.7057 | 0.6171 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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