Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of the mitochondrial alpha-ketoglutarate dehydrogenase; recruits E3 subunit (Lpd1p) to the E1-E2 (Kgd1p, Kgd2p) core; has similarity to human mitochondrial ribosomal protein MRP-S36 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 1 | 1 | 1 | 1 | 0 | – | 0 | 0 | 1 | 1 | 1 | 6 | 0 | 1 | 2 | – | 0 | 19 | 13 | 3 | 13 | 22 | 23 |
Bud | 13 | 2 | 22 | 33 | 21 | 22 | – | 29 | 20 | 41 | 18 | 36 | 32 | 11 | 12 | 6 | – | 2 | 3 | 2 | 0 | 3 | 4 | 6 |
Bud Neck | 1 | 0 | 0 | 3 | 8 | 8 | – | 1 | 3 | 4 | 1 | 2 | 3 | 1 | 0 | 0 | – | 0 | 1 | 0 | 1 | 1 | 4 | 2 |
Bud Site | 5 | 0 | 8 | 20 | 3 | 16 | – | 7 | 9 | 11 | 16 | 17 | 2 | 1 | 1 | 0 | – | 0 | – | – | – | – | – | – |
Cell Periphery | 14 | 18 | 10 | 0 | 0 | 0 | – | 1 | 1 | 4 | 1 | 4 | 10 | 5 | 7 | 3 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 6 | 3 | 6 | 6 | 6 | 10 | – | 8 | 3 | 7 | 7 | 5 | 7 | 7 | 7 | 4 | – | 5 | 0 | 0 | 1 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 1 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | – | 1 | 0 | 0 | 1 | 0 | 0 | 1 |
Endosome | 52 | 7 | 6 | 0 | 19 | 7 | – | 8 | 1 | 4 | 1 | 2 | 108 | 75 | 86 | 4 | – | 3 | 61 | 13 | 23 | 56 | 89 | 77 |
Golgi | 8 | 14 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 3 | 21 | 26 | 34 | – | 6 | 60 | 26 | 14 | 33 | 44 | 39 |
Mitochondria | 693 | 293 | 340 | 56 | 79 | 258 | – | 361 | 280 | 331 | 304 | 384 | 303 | 355 | 335 | 21 | – | 5 | 582 | 260 | 258 | 131 | 151 | 176 |
Nucleus | 5 | 2 | 0 | 0 | 2 | 4 | – | 5 | 3 | 1 | 0 | 3 | 3 | 2 | 1 | 2 | – | 0 | 1 | 1 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 25 | 13 | 10 | 0 | 0 | 7 | – | 8 | 10 | 10 | 7 | 10 | 3 | 7 | 5 | 0 | – | 0 | 8 | 4 | 2 | 1 | 1 | 3 |
Nucleolus | 44 | 9 | 17 | 20 | 39 | 90 | – | 79 | 55 | 77 | 64 | 50 | 26 | 21 | 19 | 6 | – | 4 | 4 | 1 | 0 | 2 | 4 | 4 |
Peroxisomes | 61 | 17 | 14 | 184 | 155 | 128 | – | 101 | 62 | 70 | 53 | 79 | 281 | 135 | 163 | 56 | – | 17 | 42 | 18 | 43 | 140 | 208 | 199 |
SpindlePole | 27 | 2 | 10 | 130 | 157 | 100 | – | 79 | 57 | 64 | 45 | 44 | 139 | 40 | 53 | 25 | – | 14 | 17 | 1 | 3 | 75 | 86 | 117 |
Vac/Vac Membrane | 114 | 43 | 14 | 0 | 3 | 10 | – | 9 | 5 | 7 | 4 | 7 | 25 | 34 | 39 | 6 | – | 10 | 37 | 8 | 1 | 5 | 7 | 5 |
Unique Cell Count | 815 | 335 | 353 | 274 | 344 | 469 | 522 | 380 | 469 | 413 | 498 | 608 | 511 | 546 | 138 | 49 | 850 | 354 | 359 | 475 | 640 | 675 | ||
Labelled Cell Count | 1070 | 424 | 459 | 454 | 493 | 660 | 696 | 509 | 632 | 522 | 644 | 952 | 715 | 755 | 171 | 67 | 850 | 354 | 359 | 475 | 640 | 675 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.9 | 9.7 | 5.4 | 6.8 | 8.1 | 7.5 | – | 8.5 | 8.4 | 9.0 | 9.5 | 8.9 | 8.3 | 9.2 | 9.3 | 17.9 | – | 15.8 | 9.2 | 9.4 | 9.1 |
Std Deviation (1e-4) | 1.7 | 2.0 | 1.2 | 1.9 | 2.2 | 2.6 | – | 2.7 | 3.1 | 3.4 | 3.9 | 3.7 | 1.8 | 2.1 | 2.4 | 5.5 | – | 4.6 | 5.8 | 3.8 | 2.1 |
Intensity Change (Log2) | – | – | – | 0.32 | 0.57 | 0.46 | – | 0.64 | 0.62 | 0.73 | 0.81 | 0.72 | 0.6 | 0.76 | 0.77 | 1.72 | – | 1.54 | 0.75 | 0.79 | 0.74 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Bud | 2.6 | -0.1 | -1.0 | – | -0.4 | -0.6 | 1.3 | -1.2 | 0.6 | -0.6 | -3.1 | -3.1 | -0.8 | – | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Bud Site | 3.0 | -1.5 | 1.0 | – | -1.0 | 0.1 | 0.1 | 1.3 | 1.0 | 0 | 0 | 0 | 0 | – | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | -2.9 | -2.2 | -3.0 | -2.3 | -1.2 | -2.1 | -1.7 | 0 | – | 0 |
Cytoplasm | 0.4 | 0 | 0.4 | – | -0.2 | 0 | -0.2 | 0 | 0 | 0 | -0.4 | -0.5 | 0 | – | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Endosome | 0 | 2.7 | -0.2 | – | -0.2 | 0 | 0 | 0 | 0 | 7.4 | 6.4 | 6.8 | 0 | – | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 3.9 | 4.2 | 9.7 | – | 0 |
Mitochondria | -19.5 | -19.8 | -13.2 | – | -9.9 | -8.5 | -9.4 | -8.6 | -7.7 | -14.8 | -9.8 | -11.8 | -18.3 | – | -16.2 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Nuclear Periphery | 0 | 0 | -1.3 | – | -1.3 | -0.2 | -0.6 | -1.1 | -0.8 | 0 | -1.5 | -2.2 | 0 | – | 0 |
Nucleolus | 1.3 | 3.2 | 6.1 | – | 4.8 | 4.4 | 5.2 | 4.8 | 2.8 | -0.4 | -0.5 | -1.0 | -0.2 | – | 0 |
Peroxisomes | 16.9 | 12.7 | 8.8 | – | 6.6 | 5.5 | 5.1 | 4.3 | 5.5 | 13.7 | 8.6 | 9.5 | 10.4 | – | 0 |
SpindlePole | 13.3 | 13.2 | 7.7 | – | 5.9 | 5.7 | 5.4 | 4.3 | 3.5 | 8.3 | 3.1 | 3.9 | 5.9 | – | 0 |
Vacuole | -3.3 | -2.6 | -1.5 | – | -2.0 | -2.3 | -2.2 | -2.7 | -2.4 | 0.1 | 1.7 | 2.0 | 0.2 | – | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.6392 | 7.8841 | 7.205 | 5.6696 | 4.9692 | 7.0937 | 5.6625 | 6.6069 | 6.3688 | 4.9468 | 4.586 | 6.2158 | 7.8234 | 7.7957 | 6.8317 | 6.5655 | 5.1802 | 7.5173 |
Actin | 0.0044 | 0.0065 | 0.0201 | 0.0355 | 0.029 | 0.0034 | 0.028 | 0.0112 | 0.0197 | 0.0009 | 0.0049 | 0.0135 | 0.0629 | 0.0044 | 0.0071 | 0.0002 | 0.0011 | 0.0513 |
Bud | 0.0003 | 0.0026 | 0.0011 | 0.0059 | 0.0011 | 0.0001 | 0.0004 | 0.0024 | 0.0008 | 0.0001 | 0.0006 | 0.0002 | 0.0003 | 0.0017 | 0.0008 | 0.0002 | 0.0005 | 0.0002 |
Bud Neck | 0.0016 | 0.001 | 0.0006 | 0.0026 | 0.0016 | 0.0007 | 0.0029 | 0.0013 | 0.0072 | 0.0004 | 0.0012 | 0.0023 | 0.0012 | 0.0015 | 0.0014 | 0.0003 | 0.001 | 0.0028 |
Bud Periphery | 0.0004 | 0.002 | 0.001 | 0.0034 | 0.0014 | 0.0001 | 0.0005 | 0.0028 | 0.0008 | 0.0001 | 0.001 | 0.0003 | 0.0003 | 0.0008 | 0.0017 | 0.0002 | 0.0002 | 0.0002 |
Bud Site | 0.0023 | 0.0161 | 0.0078 | 0.0132 | 0.0079 | 0.0004 | 0.0035 | 0.0164 | 0.0104 | 0.0003 | 0.0049 | 0.001 | 0.0012 | 0.0143 | 0.0021 | 0.0015 | 0.0005 | 0.0008 |
Cell Periphery | 0.0005 | 0.0004 | 0.0003 | 0.0012 | 0.0003 | 0.0001 | 0.0005 | 0.0005 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0005 | 0.0002 | 0 | 0.0002 | 0.0001 |
Cytoplasm | 0.0011 | 0.0004 | 0.0003 | 0.0037 | 0.0008 | 0.0002 | 0.0034 | 0.0003 | 0.0004 | 0.0001 | 0.0005 | 0.0001 | 0.0034 | 0.0002 | 0.0001 | 0.0001 | 0.0012 | 0.0031 |
Cytoplasmic Foci | 0.2657 | 0.3584 | 0.3469 | 0.3246 | 0.3895 | 0.3248 | 0.3309 | 0.401 | 0.3635 | 0.4889 | 0.3993 | 0.3549 | 0.1642 | 0.3533 | 0.3891 | 0.3269 | 0.4117 | 0.3271 |
Eisosomes | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.002 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0003 |
Endoplasmic Reticulum | 0.0005 | 0.0001 | 0.0002 | 0.0008 | 0.0009 | 0.0005 | 0.0011 | 0 | 0.0003 | 0 | 0.0008 | 0 | 0.0012 | 0 | 0 | 0 | 0 | 0.0022 |
Endosome | 0.0201 | 0.0161 | 0.0144 | 0.0189 | 0.0335 | 0.0138 | 0.0197 | 0.0085 | 0.0222 | 0.0077 | 0.0387 | 0.0089 | 0.0098 | 0.0091 | 0.0184 | 0.0068 | 0.0046 | 0.0284 |
Golgi | 0.0245 | 0.0346 | 0.0163 | 0.0306 | 0.033 | 0.0258 | 0.0285 | 0.0152 | 0.0284 | 0.0166 | 0.0325 | 0.0169 | 0.0207 | 0.0205 | 0.0182 | 0.0091 | 0.0084 | 0.0252 |
Lipid Particles | 0.2916 | 0.2105 | 0.182 | 0.246 | 0.2324 | 0.3038 | 0.1565 | 0.1104 | 0.1266 | 0.213 | 0.2003 | 0.225 | 0.2051 | 0.1618 | 0.1365 | 0.2987 | 0.3089 | 0.2041 |
Mitochondria | 0.1311 | 0.097 | 0.138 | 0.0831 | 0.0718 | 0.1011 | 0.1462 | 0.0713 | 0.1285 | 0.051 | 0.0838 | 0.0632 | 0.2356 | 0.1282 | 0.1128 | 0.0092 | 0.038 | 0.1674 |
None | 0.0004 | 0.0003 | 0.0004 | 0.0003 | 0.0008 | 0.0001 | 0.0041 | 0.0021 | 0.0003 | 0 | 0.0009 | 0.0001 | 0.004 | 0.0001 | 0.0001 | 0.0001 | 0.0043 | 0.0011 |
Nuclear Periphery | 0.0059 | 0.0001 | 0.0005 | 0.0008 | 0.0013 | 0.0046 | 0.0015 | 0.0003 | 0.0005 | 0.0001 | 0.0041 | 0 | 0.0142 | 0.0001 | 0.0001 | 0 | 0 | 0.0004 |
Nucleolus | 0.0142 | 0.0076 | 0.0218 | 0.0224 | 0.0271 | 0.008 | 0.0106 | 0.008 | 0.0154 | 0.0006 | 0.0104 | 0.0083 | 0.0033 | 0.0162 | 0.0203 | 0.0006 | 0.0192 | 0.0008 |
Nucleus | 0.0034 | 0.0005 | 0.0016 | 0.0033 | 0.0019 | 0.0008 | 0.001 | 0.0005 | 0.0006 | 0.0001 | 0.0019 | 0.0006 | 0.006 | 0.0018 | 0.001 | 0 | 0.0005 | 0.0001 |
Peroxisomes | 0.2197 | 0.238 | 0.2349 | 0.1928 | 0.1535 | 0.2037 | 0.249 | 0.3235 | 0.2614 | 0.1839 | 0.1931 | 0.2909 | 0.2565 | 0.272 | 0.2372 | 0.3447 | 0.1839 | 0.1813 |
Punctate Nuclear | 0.0025 | 0.0009 | 0.0051 | 0.0047 | 0.0078 | 0.0038 | 0.0064 | 0.0122 | 0.0079 | 0.0336 | 0.0147 | 0.0128 | 0.0075 | 0.0033 | 0.049 | 0.0006 | 0.014 | 0.0011 |
Vacuole | 0.0046 | 0.0044 | 0.0037 | 0.0041 | 0.0019 | 0.0014 | 0.0017 | 0.0078 | 0.0033 | 0.0008 | 0.0035 | 0.0005 | 0.0007 | 0.0042 | 0.0026 | 0.0003 | 0.0011 | 0.0008 |
Vacuole Periphery | 0.005 | 0.0025 | 0.0028 | 0.0018 | 0.0022 | 0.0026 | 0.0017 | 0.004 | 0.0013 | 0.0017 | 0.0023 | 0.0004 | 0.0015 | 0.0057 | 0.0012 | 0.0005 | 0.0006 | 0.0012 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 19.2799 | 10.6062 | 7.8703 | 31.8445 | 24.9973 | 14.8225 | 18.0966 | 26.2375 | 34.9477 | 35.639 |
Translational Efficiency | 3.169 | 4.2032 | 7.6216 | 1.9432 | 2.643 | 6.735 | 3.448 | 3.1254 | 3.0263 | 2.4921 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2044 | 1272 | 751 | 111 | 1708 | 1649 | 2224 | 1337 | 3752 | 2921 | 2975 | 1448 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 845.90 | 912.74 | 1389.04 | 1631.38 | 816.30 | 949.21 | 1037.25 | 1164.43 | 832.43 | 933.33 | 1126.05 | 1200.23 |
Standard Deviation | 142.14 | 179.36 | 270.95 | 330.48 | 126.82 | 176.90 | 253.74 | 292.65 | 136.18 | 178.89 | 300.03 | 320.76 |
Intensity Change Log 2 | — | 0.109717 | 0.715529 | 0.947534 | — | 0.217628 | 0.345592 | 0.512453 | — | 0.163720 | 0.545658 | 0.750172 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.002520 | 0.006588 | 0.014667 | 0.046936 | 0.002995 | 0.003479 | 0.002776 | 0.010600 | 0.002736 | 0.004833 | 0.005778 | 0.013385 |
Bud Neck | 0.002255 | 0.002001 | 0.007914 | 0.006560 | 0.001384 | 0.002030 | 0.002034 | 0.001714 | 0.001859 | 0.002018 | 0.003519 | 0.002085 |
Bud Site | 0.003916 | 0.002557 | 0.004094 | 0.007295 | 0.003804 | 0.003635 | 0.005179 | 0.007697 | 0.003865 | 0.003165 | 0.004905 | 0.007666 |
Cell Periphery | 0.000274 | 0.000261 | 0.000121 | 0.000596 | 0.000227 | 0.000132 | 0.000186 | 0.000345 | 0.000253 | 0.000188 | 0.000170 | 0.000364 |
Cytoplasm | 0.000748 | 0.000713 | 0.001775 | 0.000696 | 0.000816 | 0.000757 | 0.001364 | 0.001286 | 0.000779 | 0.000738 | 0.001468 | 0.001241 |
Cytoplasmic Foci | 0.012916 | 0.026120 | 0.126550 | 0.055818 | 0.018165 | 0.025468 | 0.014476 | 0.013078 | 0.015305 | 0.025752 | 0.042767 | 0.016354 |
Eisosomes | 0.000100 | 0.000197 | 0.001317 | 0.002014 | 0.000103 | 0.000119 | 0.000075 | 0.000196 | 0.000101 | 0.000153 | 0.000388 | 0.000335 |
Endoplasmic Reticulum | 0.000733 | 0.000356 | 0.000382 | 0.002043 | 0.000139 | 0.000145 | 0.000244 | 0.000362 | 0.000462 | 0.000237 | 0.000278 | 0.000491 |
Endosome | 0.021986 | 0.031521 | 0.020654 | 0.017793 | 0.023272 | 0.029110 | 0.016355 | 0.012190 | 0.022571 | 0.030160 | 0.017440 | 0.012620 |
Golgi | 0.017445 | 0.044842 | 0.133378* | 0.086694 | 0.024706 | 0.035213 | 0.025342 | 0.040757 | 0.020750 | 0.039406 | 0.052614 | 0.044278 |
Lipid Particles | 0.007740 | 0.011870 | 0.073070 | 0.014186 | 0.009128 | 0.008929 | 0.003573 | 0.001902 | 0.008372 | 0.010210 | 0.021116 | 0.002844 |
Mitochondria | 0.880876* | 0.812434* | 0.402572* | 0.692729* | 0.871201* | 0.841395* | 0.885533* | 0.865454* | 0.876472* | 0.828783* | 0.763616* | 0.852214* |
Mitotic Spindle | 0.001263 | 0.002845 | 0.035344 | 0.008872 | 0.001283 | 0.002613 | 0.011212 | 0.005977 | 0.001272 | 0.002714 | 0.017304 | 0.006199 |
None | 0.000252 | 0.000311 | 0.001177 | 0.002127 | 0.000206 | 0.000250 | 0.000355 | 0.000543 | 0.000231 | 0.000276 | 0.000562 | 0.000665 |
Nuclear Periphery | 0.000102 | 0.000085 | 0.002838 | 0.001418 | 0.000095 | 0.000133 | 0.000391 | 0.000248 | 0.000099 | 0.000112 | 0.001008 | 0.000337 |
Nuclear Periphery Foci | 0.000246 | 0.000403 | 0.001838 | 0.000641 | 0.000272 | 0.000296 | 0.000292 | 0.000174 | 0.000258 | 0.000343 | 0.000682 | 0.000210 |
Nucleolus | 0.000563 | 0.000231 | 0.000858 | 0.000208 | 0.000136 | 0.000415 | 0.000227 | 0.000113 | 0.000368 | 0.000335 | 0.000386 | 0.000120 |
Nucleus | 0.009233 | 0.003510 | 0.000142 | 0.000085 | 0.000063 | 0.000182 | 0.000384 | 0.000074 | 0.005059 | 0.001631 | 0.000323 | 0.000075 |
Peroxisomes | 0.029594 | 0.048008 | 0.153386* | 0.035766 | 0.038673 | 0.038402 | 0.017519 | 0.022418 | 0.033727 | 0.042586 | 0.051817 | 0.023442 |
Vacuole | 0.003799 | 0.002842 | 0.009073 | 0.004993 | 0.001415 | 0.003934 | 0.006070 | 0.005973 | 0.002714 | 0.003459 | 0.006828 | 0.005898 |
Vacuole Periphery | 0.003439 | 0.002305 | 0.008850 | 0.012530 | 0.001917 | 0.003362 | 0.006415 | 0.008899 | 0.002746 | 0.002902 | 0.007030 | 0.009178 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.92 | -8.85 | -4.26 | -3.86 | -3.07 | -0.57 | 0.32 | -4.83 | -4.27 | -4.93 | -3.21 | -5.18 | -6.43 | -4.97 | -4.47 |
Bud Neck | 0.46 | -7.47 | -2.80 | -2.91 | 0.85 | -1.45 | -2.01 | -0.94 | 0.67 | 0.97 | -0.43 | -5.08 | -0.67 | -0.21 | 3.93 |
Bud Site | 1.59 | -0.23 | -0.92 | -1.30 | -0.87 | 0.14 | -1.40 | -2.38 | -2.55 | -1.42 | 0.97 | -1.52 | -2.66 | -3.20 | -1.77 |
Cell Periphery | 0.19 | 3.28 | -1.68 | -1.74 | -2.56 | 2.55 | 0.61 | -1.41 | -2.79 | -1.68 | 1.68 | 1.61 | -1.44 | -2.35 | -2.35 |
Cytoplasm | 0.14 | -3.83 | 0.20 | 0.12 | 7.04 | 0.50 | -1.63 | -1.40 | -2.21 | 0.42 | 0.38 | -2.74 | -1.74 | -2.47 | 1.00 |
Cytoplasmic Foci | -5.92 | -22.04 | -4.09 | -2.80 | 6.10 | -3.15 | 2.10 | 2.73 | 5.65 | 0.87 | -6.51 | -13.95 | -0.59 | 4.85 | 11.70 |
Eisosomes | -2.61 | -12.22 | -4.42 | -4.18 | -1.57 | -1.17 | 2.86 | -3.79 | -3.06 | -5.12 | -2.83 | -10.13 | -5.55 | -4.11 | 1.07 |
Endoplasmic Reticulum | 1.48 | 1.53 | -1.81 | -2.42 | -2.42 | -0.29 | -2.01 | -2.68 | -2.63 | -1.23 | 1.68 | 1.41 | -0.18 | -2.42 | -2.12 |
Endosome | -3.32 | 0.58 | 0.85 | 2.62 | 0.58 | -2.33 | 3.66 | 5.68 | 7.34 | 2.63 | -4.01 | 3.53 | 6.15 | 9.19 | 3.32 |
Golgi | -8.49 | -18.08 | -5.47 | -3.24 | 3.31 | -3.73 | -0.25 | -4.47 | -1.42 | -4.13 | -8.91 | -12.55 | -7.18 | -1.36 | 2.14 |
Lipid Particles | -2.37 | -14.09 | -1.54 | -0.49 | 9.65 | 0.12 | 3.99 | 5.45 | 6.11 | 2.38 | -1.60 | -8.23 | 6.10 | 7.21 | 12.78 |
Mitochondria | 6.74 | 32.15* | 5.86 | 3.65 | -8.43 | 3.27 | -1.73 | 0.61 | -2.46 | 2.22 | 7.08 | 14.82 | 2.98 | -2.67 | -9.34 |
Mitotic Spindle | -2.34 | -8.08 | -3.55 | -2.72 | 5.64 | -3.27 | -7.74 | -4.99 | -3.38 | 3.41 | -3.80 | -11.08 | -5.56 | -3.74 | 6.65 |
None | -2.01 | -13.56 | -4.70 | -4.47 | -2.36 | -2.47 | -6.26 | -9.09 | -8.15 | -4.75 | -2.61 | -12.60 | -9.19 | -7.71 | -2.01 |
Nuclear Periphery | 1.03 | -5.69 | -3.18 | -3.22 | 2.24 | -1.27 | -3.09 | -2.30 | -1.80 | 1.29 | -0.81 | -6.43 | -3.56 | -3.39 | 4.32 |
Nuclear Periphery Foci | -1.21 | -5.59 | -2.45 | -0.47 | 3.76 | -0.21 | -0.17 | 0.96 | 1.63 | 1.74 | -1.19 | -4.52 | 0.73 | 1.65 | 5.08 |
Nucleolus | 0.51 | -1.42 | 2.75 | 0.93 | 3.83 | -1.74 | -1.14 | 0.44 | 2.02 | 2.00 | -0.33 | -0.19 | 3.30 | 2.25 | 4.33 |
Nucleus | 2.26 | 4.68 | 4.71 | 2.22 | 2.45 | -1.92 | -1.20 | -0.10 | 1.97 | 1.20 | 2.69 | 4.38 | 4.70 | 2.31 | 1.28 |
Peroxisomes | -4.07 | -13.29 | -0.58 | 1.09 | 8.49 | 0.06 | 6.47 | 4.13 | 3.93 | -1.59 | -2.91 | -5.43 | 3.21 | 5.42 | 7.49 |
Vacuole | 1.40 | -5.55 | -0.80 | -1.43 | 2.49 | -4.77 | -8.51 | -4.69 | -1.89 | 0.14 | -1.67 | -8.06 | -3.44 | -2.54 | 0.97 |
Vacuole Periphery | 2.06 | -4.35 | -1.62 | -1.82 | -0.64 | -3.68 | -7.16 | -5.56 | -4.35 | -1.82 | -0.44 | -7.24 | -5.15 | -5.08 | -1.62 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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