ORF | |
---|---|
Human Ortholog | |
Description | Putative methyltransferase; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; not an essential gene; YMR310C has a paralog, YGR283C, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 |
Bud | 1 | 0 | 1 | 1 | 3 | 5 | 18 | 15 | 24 | 16 | 20 | 13 | 1 | 0 | 0 | 3 | 1 | 2 | 1 | 2 | 1 | 0 | 4 | 5 |
Bud Neck | 1 | 0 | 6 | 12 | 26 | 15 | 18 | 16 | 11 | 22 | 7 | 14 | 26 | 14 | 15 | 1 | 2 | 0 | 2 | 0 | 1 | 6 | 7 | 10 |
Bud Site | 0 | 0 | 0 | 3 | 0 | 4 | 11 | 5 | 8 | 11 | 5 | 5 | 0 | 0 | 0 | 0 | 5 | 2 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 28 | 27 | 48 | 33 | 76 | 98 | 152 | 145 | 122 | 114 | 87 | 92 | 69 | 62 | 73 | 32 | 26 | 21 | 25 | 16 | 23 | 15 | 38 | 26 |
Endoplasmic Reticulum | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 5 | 6 | 7 | 0 | 0 | 2 | 0 | 1 | 1 |
Endosome | 1 | 21 | 6 | 2 | 10 | 17 | 4 | 5 | 1 | 3 | 0 | 3 | 5 | 37 | 51 | 29 | 42 | 23 | 21 | 19 | 15 | 20 | 27 | 34 |
Golgi | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 2 |
Mitochondria | 99 | 15 | 6 | 12 | 8 | 24 | 137 | 64 | 139 | 133 | 155 | 123 | 2 | 1 | 2 | 13 | 13 | 12 | 9 | 9 | 23 | 10 | 12 | 10 |
Nucleus | 7 | 16 | 13 | 4 | 1 | 1 | 6 | 7 | 3 | 7 | 5 | 4 | 29 | 8 | 5 | 18 | 31 | 18 | 16 | 24 | 8 | 2 | 3 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 2 |
Nucleolus | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 0 | 10 | 25 | 7 | 11 | 15 | 13 | 10 | 20 | 13 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 6 | 4 | 2 | 1 | 0 | 0 | 2 | 0 | 1 | 6 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 5 | 6 | 18 | 54 | 51 | 66 | 62 | 46 | 43 | 39 | 21 | 50 | 55 | 76 | 75 | 55 | 66 | 28 | 33 | 30 | 32 | 56 | 78 | 80 |
Vac/Vac Membrane | 9 | 42 | 4 | 2 | 1 | 1 | 3 | 5 | 1 | 4 | 2 | 2 | 2 | 3 | 3 | 9 | 10 | 11 | 38 | 33 | 27 | 12 | 17 | 23 |
Unique Cell Count | 130 | 107 | 96 | 107 | 151 | 196 | 330 | 240 | 265 | 263 | 228 | 231 | 174 | 182 | 200 | 131 | 158 | 98 | 177 | 162 | 166 | 145 | 227 | 228 |
Labelled Cell Count | 154 | 131 | 103 | 124 | 177 | 237 | 415 | 313 | 354 | 354 | 303 | 309 | 193 | 204 | 233 | 177 | 230 | 131 | 177 | 162 | 166 | 145 | 227 | 228 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.2 | 4.5 | 4.9 | 4.7 | 4.8 | 4.1 | 3.5 | 4.0 | 3.2 | 3.3 | 3.0 | 3.4 | 4.9 | 5.2 | 5.0 | 6.8 | 6.9 | 6.2 | 4.7 | 5.2 | 5.6 |
Std Deviation (1e-4) | 0.4 | 0.8 | 1.5 | 1.8 | 1.9 | 2.1 | 1.8 | 1.7 | 1.6 | 1.3 | 1.4 | 1.2 | 1.0 | 1.2 | 1.3 | 1.7 | 2.1 | 1.8 | 1.3 | 1.8 | 2.1 |
Intensity Change (Log2) | – | – | – | -0.04 | -0.03 | -0.26 | -0.48 | -0.27 | -0.59 | -0.55 | -0.68 | -0.52 | -0.0 | 0.11 | 0.04 | 0.47 | 0.51 | 0.35 | -0.06 | 0.1 | 0.21 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 0.9 | 0.7 | 1.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.0065 | -0.5669 | -0.6229 | -0.7827 | -0.666 | -0.6393 | -0.1653 | -0.3038 | -0.1454 | -0.348 | -0.6429 | -0.1746 | -1.0379 | -0.8433 | -1.0789 | -0.0163 | -0.4433 | -0.9918 |
Actin | 0.0169 | 0.0013 | 0.0137 | 0.0105 | 0.0179 | 0.0027 | 0.0365 | 0.0002 | 0.0117 | 0.0002 | 0.0004 | 0.0074 | 0.0095 | 0.0001 | 0.0003 | 0.0432 | 0.0478 | 0.0071 |
Bud | 0.0007 | 0.0084 | 0.0032 | 0.0008 | 0.0024 | 0.0037 | 0.0004 | 0.0003 | 0.0003 | 0.0007 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0.0007 | 0.0029 | 0.0003 |
Bud Neck | 0.0131 | 0.005 | 0.0048 | 0.0077 | 0.0269 | 0.0071 | 0.0091 | 0.0095 | 0.0065 | 0.0085 | 0.0078 | 0.0102 | 0.0101 | 0.0075 | 0.0115 | 0.0054 | 0.0098 | 0.0137 |
Bud Periphery | 0.0016 | 0.0015 | 0.0015 | 0.0012 | 0.0075 | 0.0009 | 0.0007 | 0.0004 | 0.0006 | 0.0018 | 0.0001 | 0.0005 | 0.0005 | 0.0003 | 0.0005 | 0.0016 | 0.0081 | 0.0005 |
Bud Site | 0.0094 | 0.0137 | 0.006 | 0.0053 | 0.027 | 0.001 | 0.0034 | 0.0102 | 0.006 | 0.0057 | 0.0014 | 0.0016 | 0.0046 | 0.0071 | 0.0056 | 0.004 | 0.0094 | 0.0024 |
Cell Periphery | 0.0005 | 0.0005 | 0.0005 | 0.0005 | 0.0009 | 0.0002 | 0.0004 | 0.0004 | 0.0005 | 0.0009 | 0.0001 | 0.0003 | 0.0005 | 0.0004 | 0.0006 | 0.0004 | 0.0008 | 0.0003 |
Cytoplasm | 0.0182 | 0.0237 | 0.0291 | 0.0132 | 0.0104 | 0.014 | 0.0152 | 0.022 | 0.0129 | 0.0049 | 0.0135 | 0.0143 | 0.0135 | 0.0288 | 0.0185 | 0.0164 | 0.0693 | 0.019 |
Cytoplasmic Foci | 0.0804 | 0.0279 | 0.0331 | 0.0538 | 0.0297 | 0.0376 | 0.0578 | 0.0525 | 0.0397 | 0.0297 | 0.1397 | 0.0354 | 0.0574 | 0.0494 | 0.0507 | 0.0483 | 0.0609 | 0.041 |
Eisosomes | 0.0004 | 0.0001 | 0.0003 | 0.0001 | 0.0005 | 0.0001 | 0.0008 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0008 | 0.0009 | 0.0002 |
Endoplasmic Reticulum | 0.0045 | 0.0022 | 0.0024 | 0.0009 | 0.0051 | 0.0013 | 0.0024 | 0.0012 | 0.002 | 0.0009 | 0.0009 | 0.0012 | 0.0015 | 0.0017 | 0.0014 | 0.0022 | 0.0025 | 0.001 |
Endosome | 0.0727 | 0.0232 | 0.037 | 0.0534 | 0.0295 | 0.0187 | 0.0302 | 0.0185 | 0.0254 | 0.0199 | 0.027 | 0.0168 | 0.0377 | 0.035 | 0.0299 | 0.0257 | 0.0242 | 0.0173 |
Golgi | 0.0174 | 0.0033 | 0.0054 | 0.0254 | 0.0127 | 0.0047 | 0.007 | 0.0024 | 0.0032 | 0.0027 | 0.0034 | 0.0025 | 0.005 | 0.0039 | 0.0031 | 0.0091 | 0.0115 | 0.0045 |
Lipid Particles | 0.1263 | 0.0482 | 0.0547 | 0.0856 | 0.02 | 0.0749 | 0.0605 | 0.0602 | 0.054 | 0.1318 | 0.054 | 0.0473 | 0.079 | 0.0807 | 0.0932 | 0.0456 | 0.0368 | 0.0754 |
Mitochondria | 0.0092 | 0.0028 | 0.0106 | 0.0089 | 0.0409 | 0.0044 | 0.0037 | 0.001 | 0.0018 | 0.0022 | 0.0009 | 0.0015 | 0.0008 | 0.0005 | 0.0014 | 0.0029 | 0.0177 | 0.0013 |
None | 0.0606 | 0.073 | 0.0983 | 0.0497 | 0.0997 | 0.0756 | 0.079 | 0.084 | 0.0719 | 0.0757 | 0.034 | 0.0643 | 0.0508 | 0.0379 | 0.0332 | 0.1568 | 0.0429 | 0.0394 |
Nuclear Periphery | 0.0387 | 0.0196 | 0.013 | 0.005 | 0.0334 | 0.0169 | 0.0233 | 0.0116 | 0.0085 | 0.0074 | 0.0133 | 0.017 | 0.0172 | 0.0099 | 0.0095 | 0.0181 | 0.0176 | 0.0104 |
Nucleolus | 0.1572 | 0.2965 | 0.2848 | 0.2802 | 0.1755 | 0.2443 | 0.1952 | 0.3195 | 0.4278 | 0.4026 | 0.1578 | 0.2316 | 0.2599 | 0.3002 | 0.3515 | 0.3001 | 0.1378 | 0.2421 |
Nucleus | 0.1144 | 0.2128 | 0.156 | 0.1839 | 0.2705 | 0.1328 | 0.1396 | 0.1798 | 0.1556 | 0.0872 | 0.0796 | 0.2122 | 0.1192 | 0.1966 | 0.141 | 0.158 | 0.1554 | 0.1332 |
Peroxisomes | 0.0361 | 0.0027 | 0.0076 | 0.0114 | 0.003 | 0.0071 | 0.0197 | 0.006 | 0.0031 | 0.0096 | 0.0111 | 0.0068 | 0.0095 | 0.0079 | 0.0095 | 0.0102 | 0.0155 | 0.0071 |
Punctate Nuclear | 0.2064 | 0.2214 | 0.226 | 0.1863 | 0.1741 | 0.3472 | 0.3081 | 0.2128 | 0.1621 | 0.192 | 0.4523 | 0.3221 | 0.3148 | 0.2204 | 0.2226 | 0.1481 | 0.3174 | 0.3793 |
Vacuole | 0.0095 | 0.0114 | 0.0107 | 0.0134 | 0.0085 | 0.0037 | 0.0059 | 0.0067 | 0.0057 | 0.014 | 0.0019 | 0.006 | 0.0074 | 0.0108 | 0.0143 | 0.0019 | 0.0082 | 0.004 |
Vacuole Periphery | 0.0056 | 0.0009 | 0.0014 | 0.0026 | 0.0039 | 0.001 | 0.0013 | 0.0006 | 0.0006 | 0.0014 | 0.0006 | 0.0007 | 0.0007 | 0.0008 | 0.0013 | 0.0007 | 0.0027 | 0.0006 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 30.0718 | 13.4412 | 19.1807 | 25.6141 | 22.4939 | 15.0276 | 16.7266 | 18.3452 | 12.3383 | 24.4734 |
Translational Efficiency | 1.0108 | 1.9974 | 1.5447 | 0.5674 | 0.9752 | 1.3818 | 1.3224 | 0.8625 | 1.4226 | 1.0685 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
151 | 697 | 1866 | 1801 | 913 | 1823 | 158 | 1481 | 1064 | 2520 | 2024 | 3282 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 708.10 | 752.06 | 748.89 | 924.64 | 660.11 | 644.36 | 934.58 | 899.69 | 666.92 | 674.15 | 763.39 | 913.38 |
Standard Deviation | 77.53 | 96.92 | 100.64 | 123.36 | 76.04 | 83.34 | 100.57 | 130.51 | 78.07 | 99.72 | 112.29 | 127.24 |
Intensity Change Log 2 | — | 0.086895 | 0.080801 | 0.384939 | — | -0.034840 | 0.501612 | 0.446722 | — | 0.029443 | 0.299148 | 0.415077 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000139 | 0.000787 | 0.000453 | 0.000758 | 0.000124 | 0.000209 | 0.001261 | 0.001241 | 0.000126 | 0.000369 | 0.000516 | 0.000976 |
Bud Neck | 0.005488 | 0.020213 | 0.019085 | 0.031366 | 0.005164 | 0.011253 | 0.006972 | 0.030232 | 0.005210 | 0.013731 | 0.018139 | 0.030854 |
Bud Site | 0.000822 | 0.007944 | 0.005124 | 0.005020 | 0.002555 | 0.003260 | 0.007115 | 0.014866 | 0.002309 | 0.004556 | 0.005280 | 0.009463 |
Cell Periphery | 0.000192 | 0.000195 | 0.000087 | 0.000068 | 0.000149 | 0.000174 | 0.000138 | 0.000151 | 0.000155 | 0.000180 | 0.000091 | 0.000106 |
Cytoplasm | 0.286468* | 0.120796* | 0.061422 | 0.003131 | 0.287191* | 0.207552* | 0.019375 | 0.025138 | 0.287089* | 0.183556* | 0.058140 | 0.013062 |
Cytoplasmic Foci | 0.235698* | 0.106702 | 0.002677 | 0.001367 | 0.196872* | 0.117913 | 0.004102 | 0.004274 | 0.202383* | 0.114812 | 0.002788 | 0.002678 |
Eisosomes | 0.000190 | 0.000149 | 0.000041 | 0.000047 | 0.000142 | 0.000133 | 0.000155 | 0.000065 | 0.000149 | 0.000137 | 0.000050 | 0.000055 |
Endoplasmic Reticulum | 0.001391 | 0.004794 | 0.002556 | 0.003233 | 0.001264 | 0.001671 | 0.006523 | 0.004029 | 0.001282 | 0.002535 | 0.002866 | 0.003592 |
Endosome | 0.010043 | 0.020008 | 0.002354 | 0.003122 | 0.011616 | 0.013598 | 0.003179 | 0.006833 | 0.011393 | 0.015371 | 0.002419 | 0.004797 |
Golgi | 0.001485 | 0.008283 | 0.000372 | 0.001184 | 0.001775 | 0.004145 | 0.000766 | 0.004475 | 0.001734 | 0.005289 | 0.000403 | 0.002669 |
Lipid Particles | 0.058278 | 0.031860 | 0.000978 | 0.000296 | 0.036250 | 0.037357 | 0.003190 | 0.000750 | 0.039377 | 0.035836 | 0.001151 | 0.000501 |
Mitochondria | 0.008595 | 0.031212 | 0.001912 | 0.004023 | 0.007340 | 0.018525 | 0.002973 | 0.004937 | 0.007518 | 0.022034 | 0.001995 | 0.004435 |
Mitotic Spindle | 0.001306 | 0.050434 | 0.016233 | 0.094173 | 0.005134 | 0.011435 | 0.013917 | 0.102655* | 0.004591 | 0.022222 | 0.016052 | 0.098000 |
None | 0.009448 | 0.020576 | 0.011836 | 0.001716 | 0.014371 | 0.025003 | 0.011093 | 0.007298 | 0.013672 | 0.023779 | 0.011778 | 0.004235 |
Nuclear Periphery | 0.002680 | 0.008669 | 0.001828 | 0.004172 | 0.003167 | 0.006227 | 0.001890 | 0.002781 | 0.003098 | 0.006903 | 0.001833 | 0.003544 |
Nuclear Periphery Foci | 0.002880 | 0.016905 | 0.003991 | 0.011180 | 0.004430 | 0.009498 | 0.023589 | 0.005630 | 0.004210 | 0.011547 | 0.005521 | 0.008675 |
Nucleolus | 0.026896 | 0.171195 | 0.189830 | 0.433979* | 0.039018 | 0.126035 | 0.284803* | 0.324132* | 0.037298 | 0.138526 | 0.197244 | 0.384410* |
Nucleus | 0.316897* | 0.341172* | 0.673235* | 0.391466* | 0.364228* | 0.380497* | 0.594602* | 0.436769* | 0.357511* | 0.369620* | 0.667097* | 0.411909* |
Peroxisomes | 0.015025 | 0.020089 | 0.001767 | 0.001461 | 0.006735 | 0.014532 | 0.003918 | 0.002010 | 0.007912 | 0.016069 | 0.001935 | 0.001709 |
Vacuole | 0.015087 | 0.013195 | 0.003737 | 0.005485 | 0.011434 | 0.009587 | 0.010027 | 0.016643 | 0.011952 | 0.010585 | 0.004228 | 0.010520 |
Vacuole Periphery | 0.000991 | 0.004822 | 0.000481 | 0.002754 | 0.001039 | 0.001396 | 0.000411 | 0.005092 | 0.001032 | 0.002343 | 0.000475 | 0.003809 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -6.80 | -9.02 | -16.39 | -1.65 | -6.43 | -1.86 | -7.77 | -10.30 | -9.73 | 2.40 | -5.34 | -11.07 | -15.75 | -11.80 | -6.13 |
Bud Neck | -6.69 | -10.10 | -17.01 | -10.33 | -9.80 | -5.63 | -2.79 | -20.38 | -17.24 | -8.70 | -8.56 | -14.79 | -27.82 | -21.66 | -13.13 |
Bud Site | -3.96 | -5.35 | -8.90 | 0.16 | -1.01 | -0.48 | -2.35 | -8.56 | -8.79 | -2.07 | -1.86 | -3.45 | -8.67 | -7.66 | -5.00 |
Cell Periphery | 0.09 | 4.20 | 4.62 | 7.36 | 1.38 | -1.49 | -0.78 | 1.97 | 3.18 | 1.67 | -1.64 | 7.23 | 6.61 | 8.66 | -0.88 |
Cytoplasm | 7.54 | 10.92 | 13.34 | 15.53 | 15.86 | 8.37 | 28.44 | 29.53 | 27.58 | -1.89 | 11.90 | 28.47 | 34.52 | 33.82 | 11.49 |
Cytoplasmic Foci | 6.75 | 12.83 | 12.94 | 18.55 | 4.40 | 10.14 | 29.94 | 30.64 | 30.98 | 1.76 | 12.51 | 33.27 | 33.45 | 36.39 | 2.42 |
Eisosomes | 1.58 | 6.12 | 6.28 | 17.02 | 1.37 | 1.70 | -2.88 | 14.45 | 14.72 | 6.84 | 1.99 | 15.90 | 17.88 | 23.94 | 1.45 |
Endoplasmic Reticulum | -5.13 | -3.21 | -4.29 | 1.91 | -2.39 | -2.62 | -9.63 | -14.32 | -13.02 | 4.94 | -7.00 | -12.94 | -16.04 | -9.86 | -2.83 |
Endosome | -4.14 | 5.53 | 5.20 | 10.26 | -0.57 | -0.71 | 3.54 | 5.17 | 8.69 | 0.89 | -2.16 | 8.23 | 7.10 | 14.31 | -2.81 |
Golgi | -5.89 | 3.27 | 1.11 | 6.44 | -3.99 | -2.63 | 1.41 | -1.19 | 1.07 | -2.17 | -5.00 | 4.05 | -0.07 | 4.98 | -4.27 |
Lipid Particles | 2.64 | 5.80 | 5.87 | 11.96 | 6.12 | -0.63 | 13.82 | 15.75 | 21.94 | 5.68 | 1.16 | 15.95 | 16.27 | 25.05 | 6.45 |
Mitochondria | -4.90 | 2.16 | 1.40 | 8.53 | -5.55 | -4.87 | 2.68 | 2.22 | 8.01 | -1.34 | -7.01 | 4.34 | 2.57 | 11.38 | -6.76 |
Mitotic Spindle | -8.66 | -7.62 | -19.47 | -8.37 | -15.82 | -2.83 | -2.69 | -18.25 | -17.51 | -9.95 | -7.44 | -6.95 | -25.77 | -21.24 | -21.17 |
None | -4.16 | -1.37 | 5.12 | 8.94 | 17.15 | -4.93 | 1.65 | 5.35 | 12.08 | 1.60 | -5.39 | 2.12 | 7.85 | 16.47 | 12.04 |
Nuclear Periphery | -1.87 | 1.30 | -0.60 | 1.94 | -7.52 | -4.20 | 1.76 | 0.50 | 4.50 | -1.22 | -4.71 | 2.82 | -1.66 | 3.95 | -6.52 |
Nuclear Periphery Foci | -7.41 | -2.68 | -7.17 | 2.97 | -5.02 | -3.61 | -4.24 | -0.85 | 3.09 | 4.08 | -6.50 | -2.29 | -4.66 | 2.55 | -2.15 |
Nucleolus | -14.73* | -23.05* | -41.09* | -18.39* | -18.01 | -14.52* | -11.09 | -29.03* | -16.47 | -0.25 | -19.08* | -26.59* | -48.95* | -28.58* | -14.40 |
Nucleus | -0.27 | -13.28 | -2.39 | -3.78 | 25.81 | -0.50 | -6.86 | -4.52 | -4.67 | 4.86 | -0.09 | -24.32 | -3.99 | -4.98 | 26.42 |
Peroxisomes | -1.01 | 3.95 | 4.02 | 11.84 | 0.29 | -5.91 | 2.09 | 6.63 | 12.70 | 3.10 | -6.41 | 7.13 | 8.03 | 17.12 | 0.31 |
Vacuole | 1.43 | 4.84 | 3.73 | 3.78 | -2.26 | 2.42 | -2.18 | -6.14 | -7.02 | -1.30 | 2.32 | 6.47 | -3.02 | -4.59 | -7.31 |
Vacuole Periphery | -4.67 | 2.38 | -2.99 | 2.39 | -4.57 | -0.98 | 1.70 | -3.68 | -3.34 | -4.64 | -3.77 | 2.65 | -4.50 | -1.61 | -6.65 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|