ORF
Human Ortholog
Description Putative methyltransferase; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; not an essential gene; YMR310C has a paralog, YGR283C, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.05 0.06 0.09 0.06 0.09 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.06 0.11 0.17 0.08 0.05 0.07 0 0.08 0 0.06 0.15 0.08 0.07 0 0 0 0 0 0 0.05 0 0.05
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.22 0.25 0.5 0.31 0.5 0.5 0.46 0.6 0.46 0.43 0.38 0.4 0.4 0.34 0.36 0.24 0.16 0.21 0.14 0.1 0.14 0.11 0.17 0.12
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Endosome 0 0.2 0.06 0 0.07 0.09 0 0 0 0 0 0 0 0.2 0.26 0.22 0.27 0.23 0.12 0.12 0.1 0.14 0.12 0.15
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.76 0.14 0.06 0.11 0.05 0.12 0.42 0.27 0.52 0.51 0.68 0.53 0 0 0 0.1 0.08 0.12 0.05 0.06 0.14 0.07 0.05 0
Nucleus 0.05 0.15 0.14 0 0 0 0 0 0 0 0 0 0.17 0 0 0.14 0.2 0.18 0.09 0.15 0.05 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.16 0.07 0.07 0.1 0.08 0.07 0.09 0.06
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0.06 0.19 0.5 0.34 0.34 0.19 0.19 0.16 0.15 0.09 0.22 0.32 0.42 0.38 0.42 0.42 0.29 0.19 0.19 0.2 0.39 0.35 0.35
Vac/Vac Membrane 0.07 0.39 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0.11 0.22 0.21 0.17 0.08 0.08 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 2 0 0 2 0 1 0 0 0 0 2 0 0 1 0
Bud 1 0 1 1 3 5 18 15 24 16 20 13 1 0 0 3 1 2 1 2 1 0 4 5
Bud Neck 1 0 6 12 26 15 18 16 11 22 7 14 26 14 15 1 2 0 2 0 1 6 7 10
Bud Site 0 0 0 3 0 4 11 5 8 11 5 5 0 0 0 0 5 2
Cell Periphery 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 28 27 48 33 76 98 152 145 122 114 87 92 69 62 73 32 26 21 25 16 23 15 38 26
Endoplasmic Reticulum 0 2 0 0 0 0 0 2 0 0 0 0 2 1 2 5 6 7 0 0 2 0 1 1
Endosome 1 21 6 2 10 17 4 5 1 3 0 3 5 37 51 29 42 23 21 19 15 20 27 34
Golgi 1 1 1 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1 1 0 2
Mitochondria 99 15 6 12 8 24 137 64 139 133 155 123 2 1 2 13 13 12 9 9 23 10 12 10
Nucleus 7 16 13 4 1 1 6 7 3 7 5 4 29 8 5 18 31 18 16 24 8 2 3 1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 2 2
Nucleolus 2 1 0 1 0 0 0 0 1 1 0 1 0 1 0 10 25 7 11 15 13 10 20 13
Peroxisomes 0 0 0 0 0 6 4 2 1 0 0 2 0 1 6 0 2 0 0 0 0 0 0 1
SpindlePole 5 6 18 54 51 66 62 46 43 39 21 50 55 76 75 55 66 28 33 30 32 56 78 80
Vac/Vac Membrane 9 42 4 2 1 1 3 5 1 4 2 2 2 3 3 9 10 11 38 33 27 12 17 23
Unique Cell Count 130 107 96 107 151 196 330 240 265 263 228 231 174 182 200 131 158 98 177 162 166 145 227 228
Labelled Cell Count 154 131 103 124 177 237 415 313 354 354 303 309 193 204 233 177 230 131 177 162 166 145 227 228


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.2 4.5 4.9 4.7 4.8 4.1 3.5 4.0 3.2 3.3 3.0 3.4 4.9 5.2 5.0 6.8 6.9 6.2 4.7 5.2 5.6
Std Deviation (1e-4) 0.4 0.8 1.5 1.8 1.9 2.1 1.8 1.7 1.6 1.3 1.4 1.2 1.0 1.2 1.3 1.7 2.1 1.8 1.3 1.8 2.1
Intensity Change (Log2) -0.04 -0.03 -0.26 -0.48 -0.27 -0.59 -0.55 -0.68 -0.52 -0.0 0.11 0.04 0.47 0.51 0.35 -0.06 0.1 0.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 0.9 0.7 1.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.0065 -0.5669 -0.6229 -0.7827 -0.666 -0.6393 -0.1653 -0.3038 -0.1454 -0.348 -0.6429 -0.1746 -1.0379 -0.8433 -1.0789 -0.0163 -0.4433 -0.9918
Actin 0.0169 0.0013 0.0137 0.0105 0.0179 0.0027 0.0365 0.0002 0.0117 0.0002 0.0004 0.0074 0.0095 0.0001 0.0003 0.0432 0.0478 0.0071
Bud 0.0007 0.0084 0.0032 0.0008 0.0024 0.0037 0.0004 0.0003 0.0003 0.0007 0.0001 0.0002 0.0002 0.0002 0.0003 0.0007 0.0029 0.0003
Bud Neck 0.0131 0.005 0.0048 0.0077 0.0269 0.0071 0.0091 0.0095 0.0065 0.0085 0.0078 0.0102 0.0101 0.0075 0.0115 0.0054 0.0098 0.0137
Bud Periphery 0.0016 0.0015 0.0015 0.0012 0.0075 0.0009 0.0007 0.0004 0.0006 0.0018 0.0001 0.0005 0.0005 0.0003 0.0005 0.0016 0.0081 0.0005
Bud Site 0.0094 0.0137 0.006 0.0053 0.027 0.001 0.0034 0.0102 0.006 0.0057 0.0014 0.0016 0.0046 0.0071 0.0056 0.004 0.0094 0.0024
Cell Periphery 0.0005 0.0005 0.0005 0.0005 0.0009 0.0002 0.0004 0.0004 0.0005 0.0009 0.0001 0.0003 0.0005 0.0004 0.0006 0.0004 0.0008 0.0003
Cytoplasm 0.0182 0.0237 0.0291 0.0132 0.0104 0.014 0.0152 0.022 0.0129 0.0049 0.0135 0.0143 0.0135 0.0288 0.0185 0.0164 0.0693 0.019
Cytoplasmic Foci 0.0804 0.0279 0.0331 0.0538 0.0297 0.0376 0.0578 0.0525 0.0397 0.0297 0.1397 0.0354 0.0574 0.0494 0.0507 0.0483 0.0609 0.041
Eisosomes 0.0004 0.0001 0.0003 0.0001 0.0005 0.0001 0.0008 0.0001 0.0002 0.0002 0.0001 0.0002 0.0002 0.0001 0.0001 0.0008 0.0009 0.0002
Endoplasmic Reticulum 0.0045 0.0022 0.0024 0.0009 0.0051 0.0013 0.0024 0.0012 0.002 0.0009 0.0009 0.0012 0.0015 0.0017 0.0014 0.0022 0.0025 0.001
Endosome 0.0727 0.0232 0.037 0.0534 0.0295 0.0187 0.0302 0.0185 0.0254 0.0199 0.027 0.0168 0.0377 0.035 0.0299 0.0257 0.0242 0.0173
Golgi 0.0174 0.0033 0.0054 0.0254 0.0127 0.0047 0.007 0.0024 0.0032 0.0027 0.0034 0.0025 0.005 0.0039 0.0031 0.0091 0.0115 0.0045
Lipid Particles 0.1263 0.0482 0.0547 0.0856 0.02 0.0749 0.0605 0.0602 0.054 0.1318 0.054 0.0473 0.079 0.0807 0.0932 0.0456 0.0368 0.0754
Mitochondria 0.0092 0.0028 0.0106 0.0089 0.0409 0.0044 0.0037 0.001 0.0018 0.0022 0.0009 0.0015 0.0008 0.0005 0.0014 0.0029 0.0177 0.0013
None 0.0606 0.073 0.0983 0.0497 0.0997 0.0756 0.079 0.084 0.0719 0.0757 0.034 0.0643 0.0508 0.0379 0.0332 0.1568 0.0429 0.0394
Nuclear Periphery 0.0387 0.0196 0.013 0.005 0.0334 0.0169 0.0233 0.0116 0.0085 0.0074 0.0133 0.017 0.0172 0.0099 0.0095 0.0181 0.0176 0.0104
Nucleolus 0.1572 0.2965 0.2848 0.2802 0.1755 0.2443 0.1952 0.3195 0.4278 0.4026 0.1578 0.2316 0.2599 0.3002 0.3515 0.3001 0.1378 0.2421
Nucleus 0.1144 0.2128 0.156 0.1839 0.2705 0.1328 0.1396 0.1798 0.1556 0.0872 0.0796 0.2122 0.1192 0.1966 0.141 0.158 0.1554 0.1332
Peroxisomes 0.0361 0.0027 0.0076 0.0114 0.003 0.0071 0.0197 0.006 0.0031 0.0096 0.0111 0.0068 0.0095 0.0079 0.0095 0.0102 0.0155 0.0071
Punctate Nuclear 0.2064 0.2214 0.226 0.1863 0.1741 0.3472 0.3081 0.2128 0.1621 0.192 0.4523 0.3221 0.3148 0.2204 0.2226 0.1481 0.3174 0.3793
Vacuole 0.0095 0.0114 0.0107 0.0134 0.0085 0.0037 0.0059 0.0067 0.0057 0.014 0.0019 0.006 0.0074 0.0108 0.0143 0.0019 0.0082 0.004
Vacuole Periphery 0.0056 0.0009 0.0014 0.0026 0.0039 0.001 0.0013 0.0006 0.0006 0.0014 0.0006 0.0007 0.0007 0.0008 0.0013 0.0007 0.0027 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 30.0718 13.4412 19.1807 25.6141 22.4939 15.0276 16.7266 18.3452 12.3383 24.4734
Translational Efficiency 1.0108 1.9974 1.5447 0.5674 0.9752 1.3818 1.3224 0.8625 1.4226 1.0685

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
151 697 1866 1801 913 1823 158 1481 1064 2520 2024 3282

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 708.10 752.06 748.89 924.64 660.11 644.36 934.58 899.69 666.92 674.15 763.39 913.38
Standard Deviation 77.53 96.92 100.64 123.36 76.04 83.34 100.57 130.51 78.07 99.72 112.29 127.24
Intensity Change Log 2 0.086895 0.080801 0.384939 -0.034840 0.501612 0.446722 0.029443 0.299148 0.415077

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000139 0.000787 0.000453 0.000758 0.000124 0.000209 0.001261 0.001241 0.000126 0.000369 0.000516 0.000976
Bud Neck 0.005488 0.020213 0.019085 0.031366 0.005164 0.011253 0.006972 0.030232 0.005210 0.013731 0.018139 0.030854
Bud Site 0.000822 0.007944 0.005124 0.005020 0.002555 0.003260 0.007115 0.014866 0.002309 0.004556 0.005280 0.009463
Cell Periphery 0.000192 0.000195 0.000087 0.000068 0.000149 0.000174 0.000138 0.000151 0.000155 0.000180 0.000091 0.000106
Cytoplasm 0.286468* 0.120796* 0.061422 0.003131 0.287191* 0.207552* 0.019375 0.025138 0.287089* 0.183556* 0.058140 0.013062
Cytoplasmic Foci 0.235698* 0.106702 0.002677 0.001367 0.196872* 0.117913 0.004102 0.004274 0.202383* 0.114812 0.002788 0.002678
Eisosomes 0.000190 0.000149 0.000041 0.000047 0.000142 0.000133 0.000155 0.000065 0.000149 0.000137 0.000050 0.000055
Endoplasmic Reticulum 0.001391 0.004794 0.002556 0.003233 0.001264 0.001671 0.006523 0.004029 0.001282 0.002535 0.002866 0.003592
Endosome 0.010043 0.020008 0.002354 0.003122 0.011616 0.013598 0.003179 0.006833 0.011393 0.015371 0.002419 0.004797
Golgi 0.001485 0.008283 0.000372 0.001184 0.001775 0.004145 0.000766 0.004475 0.001734 0.005289 0.000403 0.002669
Lipid Particles 0.058278 0.031860 0.000978 0.000296 0.036250 0.037357 0.003190 0.000750 0.039377 0.035836 0.001151 0.000501
Mitochondria 0.008595 0.031212 0.001912 0.004023 0.007340 0.018525 0.002973 0.004937 0.007518 0.022034 0.001995 0.004435
Mitotic Spindle 0.001306 0.050434 0.016233 0.094173 0.005134 0.011435 0.013917 0.102655* 0.004591 0.022222 0.016052 0.098000
None 0.009448 0.020576 0.011836 0.001716 0.014371 0.025003 0.011093 0.007298 0.013672 0.023779 0.011778 0.004235
Nuclear Periphery 0.002680 0.008669 0.001828 0.004172 0.003167 0.006227 0.001890 0.002781 0.003098 0.006903 0.001833 0.003544
Nuclear Periphery Foci 0.002880 0.016905 0.003991 0.011180 0.004430 0.009498 0.023589 0.005630 0.004210 0.011547 0.005521 0.008675
Nucleolus 0.026896 0.171195 0.189830 0.433979* 0.039018 0.126035 0.284803* 0.324132* 0.037298 0.138526 0.197244 0.384410*
Nucleus 0.316897* 0.341172* 0.673235* 0.391466* 0.364228* 0.380497* 0.594602* 0.436769* 0.357511* 0.369620* 0.667097* 0.411909*
Peroxisomes 0.015025 0.020089 0.001767 0.001461 0.006735 0.014532 0.003918 0.002010 0.007912 0.016069 0.001935 0.001709
Vacuole 0.015087 0.013195 0.003737 0.005485 0.011434 0.009587 0.010027 0.016643 0.011952 0.010585 0.004228 0.010520
Vacuole Periphery 0.000991 0.004822 0.000481 0.002754 0.001039 0.001396 0.000411 0.005092 0.001032 0.002343 0.000475 0.003809

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.80 -9.02 -16.39 -1.65 -6.43 -1.86 -7.77 -10.30 -9.73 2.40 -5.34 -11.07 -15.75 -11.80 -6.13
Bud Neck -6.69 -10.10 -17.01 -10.33 -9.80 -5.63 -2.79 -20.38 -17.24 -8.70 -8.56 -14.79 -27.82 -21.66 -13.13
Bud Site -3.96 -5.35 -8.90 0.16 -1.01 -0.48 -2.35 -8.56 -8.79 -2.07 -1.86 -3.45 -8.67 -7.66 -5.00
Cell Periphery 0.09 4.20 4.62 7.36 1.38 -1.49 -0.78 1.97 3.18 1.67 -1.64 7.23 6.61 8.66 -0.88
Cytoplasm 7.54 10.92 13.34 15.53 15.86 8.37 28.44 29.53 27.58 -1.89 11.90 28.47 34.52 33.82 11.49
Cytoplasmic Foci 6.75 12.83 12.94 18.55 4.40 10.14 29.94 30.64 30.98 1.76 12.51 33.27 33.45 36.39 2.42
Eisosomes 1.58 6.12 6.28 17.02 1.37 1.70 -2.88 14.45 14.72 6.84 1.99 15.90 17.88 23.94 1.45
Endoplasmic Reticulum -5.13 -3.21 -4.29 1.91 -2.39 -2.62 -9.63 -14.32 -13.02 4.94 -7.00 -12.94 -16.04 -9.86 -2.83
Endosome -4.14 5.53 5.20 10.26 -0.57 -0.71 3.54 5.17 8.69 0.89 -2.16 8.23 7.10 14.31 -2.81
Golgi -5.89 3.27 1.11 6.44 -3.99 -2.63 1.41 -1.19 1.07 -2.17 -5.00 4.05 -0.07 4.98 -4.27
Lipid Particles 2.64 5.80 5.87 11.96 6.12 -0.63 13.82 15.75 21.94 5.68 1.16 15.95 16.27 25.05 6.45
Mitochondria -4.90 2.16 1.40 8.53 -5.55 -4.87 2.68 2.22 8.01 -1.34 -7.01 4.34 2.57 11.38 -6.76
Mitotic Spindle -8.66 -7.62 -19.47 -8.37 -15.82 -2.83 -2.69 -18.25 -17.51 -9.95 -7.44 -6.95 -25.77 -21.24 -21.17
None -4.16 -1.37 5.12 8.94 17.15 -4.93 1.65 5.35 12.08 1.60 -5.39 2.12 7.85 16.47 12.04
Nuclear Periphery -1.87 1.30 -0.60 1.94 -7.52 -4.20 1.76 0.50 4.50 -1.22 -4.71 2.82 -1.66 3.95 -6.52
Nuclear Periphery Foci -7.41 -2.68 -7.17 2.97 -5.02 -3.61 -4.24 -0.85 3.09 4.08 -6.50 -2.29 -4.66 2.55 -2.15
Nucleolus -14.73* -23.05* -41.09* -18.39* -18.01 -14.52* -11.09 -29.03* -16.47 -0.25 -19.08* -26.59* -48.95* -28.58* -14.40
Nucleus -0.27 -13.28 -2.39 -3.78 25.81 -0.50 -6.86 -4.52 -4.67 4.86 -0.09 -24.32 -3.99 -4.98 26.42
Peroxisomes -1.01 3.95 4.02 11.84 0.29 -5.91 2.09 6.63 12.70 3.10 -6.41 7.13 8.03 17.12 0.31
Vacuole 1.43 4.84 3.73 3.78 -2.26 2.42 -2.18 -6.14 -7.02 -1.30 2.32 6.47 -3.02 -4.59 -7.31
Vacuole Periphery -4.67 2.38 -2.99 2.39 -4.57 -0.98 1.70 -3.68 -3.34 -4.64 -3.77 2.65 -4.50 -1.61 -6.65
ORF
Human Ortholog
Description Putative methyltransferase; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; not an essential gene; YMR310C has a paralog, YGR283C, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (39%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

YMR310C

YMR310C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ymr310c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available