Standard name | |
---|---|
Human Ortholog | |
Description | Bifunctional triacylglycerol lipase and LPE acyltransferase; major lipid particle-localized triacylglycerol (TAG) lipase; catalyzes acylation of lysophosphatidylethanolamine (LPE), a function which is essential for sporulation; protein level and stability of Tgl3p are markedly reduced in the absence of lipid droplets; required with Tgl4p for timely bud formation |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 2 | 0 | 3 | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 9 | 1 | 0 | 1 | 2 |
Bud | 3 | 7 | 1 | 5 | 6 | 5 | 10 | 17 | 9 | 6 | 13 | 3 | 2 | 0 | 1 | 0 | 2 | 2 | 2 | 5 | 3 | 4 | 2 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 1 | 1 | 1 | 0 | 2 | 1 | 2 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud Site | 5 | 3 | 0 | 1 | 0 | 1 | 1 | 3 | 2 | 0 | 6 | 6 | 0 | 0 | 0 | 0 | 1 | 2 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 4 | 8 | 7 | 14 | 9 | 19 | 23 | 8 | 8 | 7 | 11 | 8 | 13 | 13 | 10 | 16 | 15 | 0 | 0 | 0 | 3 | 0 | 1 |
Endoplasmic Reticulum | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 5 | 1 | 0 | 0 | 1 | 0 | 0 | 1 |
Endosome | 35 | 55 | 59 | 40 | 50 | 60 | 63 | 112 | 43 | 67 | 21 | 65 | 51 | 57 | 102 | 40 | 59 | 60 | 75 | 107 | 104 | 55 | 91 | 97 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 3 | 2 | 5 | 14 | 4 | 7 | 6 | 6 |
Mitochondria | 80 | 115 | 24 | 30 | 14 | 46 | 83 | 123 | 99 | 93 | 206 | 186 | 33 | 20 | 11 | 22 | 19 | 14 | 3 | 21 | 11 | 7 | 11 | 11 |
Nucleus | 0 | 2 | 1 | 1 | 2 | 5 | 7 | 8 | 5 | 10 | 8 | 4 | 0 | 0 | 0 | 2 | 2 | 1 | 0 | 0 | 0 | 1 | 1 | 1 |
Nuclear Periphery | 1 | 5 | 0 | 1 | 1 | 0 | 0 | 8 | 1 | 7 | 6 | 14 | 0 | 0 | 0 | 2 | 0 | 3 | 1 | 1 | 0 | 1 | 0 | 1 |
Nucleolus | 46 | 51 | 29 | 34 | 36 | 63 | 85 | 136 | 80 | 84 | 115 | 110 | 26 | 34 | 55 | 61 | 90 | 70 | 0 | 6 | 2 | 2 | 2 | 1 |
Peroxisomes | 96 | 123 | 78 | 145 | 123 | 140 | 111 | 120 | 57 | 43 | 32 | 31 | 108 | 108 | 133 | 89 | 125 | 89 | 84 | 105 | 79 | 63 | 91 | 100 |
SpindlePole | 74 | 60 | 98 | 140 | 170 | 176 | 208 | 238 | 93 | 90 | 62 | 76 | 93 | 96 | 125 | 73 | 101 | 104 | 51 | 39 | 36 | 31 | 47 | 33 |
Vac/Vac Membrane | 0 | 6 | 4 | 1 | 2 | 2 | 2 | 3 | 2 | 5 | 4 | 7 | 1 | 2 | 3 | 7 | 5 | 11 | 0 | 4 | 0 | 3 | 3 | 2 |
Unique Cell Count | 212 | 293 | 220 | 268 | 309 | 334 | 416 | 546 | 270 | 288 | 346 | 359 | 198 | 216 | 271 | 196 | 287 | 252 | 235 | 327 | 255 | 190 | 271 | 272 |
Labelled Cell Count | 343 | 434 | 303 | 408 | 419 | 508 | 595 | 796 | 401 | 415 | 482 | 513 | 326 | 331 | 447 | 307 | 428 | 375 | 235 | 327 | 255 | 190 | 271 | 272 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.0 | 9.0 | 9.2 | 8.2 | 8.5 | 7.2 | 7.0 | 7.4 | 6.7 | 7.0 | 6.3 | 6.8 | 8.4 | 9.0 | 8.7 | 12.1 | 12.4 | 11.5 | 8.8 | 8.4 | 8.5 |
Std Deviation (1e-4) | 2.0 | 2.7 | 3.7 | 2.3 | 3.1 | 2.2 | 3.1 | 2.3 | 2.4 | 2.3 | 2.1 | 2.3 | 2.1 | 2.9 | 2.2 | 4.5 | 4.1 | 4.2 | 2.5 | 2.3 | 2.2 |
Intensity Change (Log2) | – | – | – | -0.17 | -0.12 | -0.35 | -0.39 | -0.31 | -0.46 | -0.39 | -0.54 | -0.44 | -0.13 | -0.03 | -0.08 | 0.4 | 0.43 | 0.32 | -0.07 | -0.13 | -0.12 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 2.2 | 0 | 0 | 2.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.7 | 0.5 | -0.6 | 0.6 | 0.4 | -0.4 | -0.5 | -1.2 | -0.4 | 0.2 | 1.2 | 0.6 | 0.7 | 1.0 | 1.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -3.3 | -3.0 | -2.5 | -3.6 | -1.9 | -3.0 | -0.9 | -6.9 | -2.5 | -0.2 | -0.1 | 2.5 | -1.5 | -1.7 | -0.8 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.1 | -2.8 | 1.0 | 2.9 | 3.7 | 6.5 | 5.7 | 11.5 | 9.9 | 1.7 | -0.6 | -2.9 | 0.1 | -1.7 | -2.1 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -0.2 | -0.5 | 1.8 | 2.3 | 3.6 | 4.4 | 4.3 | 5.3 | 4.8 | 0 | 0.8 | 2.1 | 4.4 | 4.8 | 3.9 |
Peroxisomes | 4.1 | 1.0 | 1.5 | -2.3 | -3.9 | -3.5 | -5.4 | -7.7 | -8.0 | 3.9 | 3.1 | 3.0 | 2.1 | 1.8 | 0 |
SpindlePole | 1.7 | 2.4 | 1.9 | 1.3 | -0.2 | -2.3 | -3.1 | -6.9 | -6.0 | 0.5 | 0 | 0.3 | -1.5 | -2.1 | -0.7 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.1 | 0 | 1.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.4169 | 4.3956 | 3.6772 | 4.0532 | 3.749 | 3.9161 | 2.0489 | 4.4025 | 3.6749 | 3.3919 | 3.0332 | 4.0507 | 2.6401 | 6.0794 | 4.7395 | 3.8203 | 4.9467 | 4.4277 |
Actin | 0.0022 | 0.0011 | 0.0041 | 0.0001 | 0.0002 | 0.0008 | 0.0085 | 0.0009 | 0.004 | 0.0352 | 0.0001 | 0.0001 | 0.0102 | 0.0003 | 0.0019 | 0 | 0.0055 | 0.0006 |
Bud | 0.0002 | 0.0016 | 0.0006 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0011 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 |
Bud Neck | 0.0039 | 0.0008 | 0.0009 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0003 | 0.0002 | 0.0008 | 0.0005 | 0.0001 | 0.0006 | 0.0002 | 0.0001 | 0 | 0.0005 | 0.0001 |
Bud Periphery | 0.0002 | 0.0028 | 0.0009 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0025 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0003 | 0 |
Bud Site | 0.0025 | 0.0043 | 0.0049 | 0.0001 | 0.0002 | 0.0003 | 0.0005 | 0.0003 | 0.0005 | 0.0145 | 0.0011 | 0 | 0.0004 | 0.0003 | 0.0001 | 0 | 0.0016 | 0.0001 |
Cell Periphery | 0.0002 | 0.0005 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0006 | 0.0001 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0.0003 | 0 |
Cytoplasm | 0.0016 | 0.0005 | 0.0017 | 0.0009 | 0.0002 | 0.0002 | 0.0007 | 0.0001 | 0.0003 | 0.0013 | 0.0005 | 0.0001 | 0.001 | 0 | 0.0004 | 0 | 0.0022 | 0 |
Cytoplasmic Foci | 0.2613 | 0.2688 | 0.2587 | 0.2616 | 0.2497 | 0.2079 | 0.1934 | 0.2216 | 0.2059 | 0.2027 | 0.2124 | 0.1792 | 0.1731 | 0.1917 | 0.2073 | 0.2 | 0.2581 | 0.1408 |
Eisosomes | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0004 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0011 | 0.0001 | 0.0007 | 0.0004 | 0.0001 | 0.0002 | 0.0006 | 0.0001 | 0.0002 | 0.0014 | 0.0001 | 0.0001 | 0.0006 | 0 | 0.0008 | 0 | 0.0049 | 0 |
Endosome | 0.0265 | 0.0297 | 0.0257 | 0.0364 | 0.0224 | 0.0128 | 0.0332 | 0.0121 | 0.0178 | 0.0174 | 0.0371 | 0.0152 | 0.0101 | 0.0064 | 0.0055 | 0.0131 | 0.019 | 0.002 |
Golgi | 0.0109 | 0.0271 | 0.0217 | 0.0213 | 0.019 | 0.0155 | 0.0187 | 0.0157 | 0.0168 | 0.0209 | 0.0153 | 0.0097 | 0.0086 | 0.0111 | 0.0117 | 0.0122 | 0.0071 | 0.0064 |
Lipid Particles | 0.565 | 0.5303 | 0.5012 | 0.5645 | 0.6269 | 0.6823 | 0.6172 | 0.6505 | 0.6386 | 0.5783 | 0.4805 | 0.7369 | 0.6616 | 0.6741 | 0.6796 | 0.7056 | 0.6011 | 0.8155 |
Mitochondria | 0.0117 | 0.0026 | 0.0176 | 0.0012 | 0.0013 | 0.0109 | 0.0075 | 0.0032 | 0.004 | 0.0186 | 0.0088 | 0.0018 | 0.0026 | 0.011 | 0.0059 | 0.0014 | 0.0028 | 0.0021 |
None | 0.0009 | 0.0001 | 0.0052 | 0.0005 | 0 | 0.0001 | 0.0013 | 0 | 0.0001 | 0.0032 | 0.0001 | 0 | 0.0013 | 0 | 0.0005 | 0 | 0.0014 | 0 |
Nuclear Periphery | 0.0029 | 0.0007 | 0.0009 | 0.0011 | 0.0001 | 0.0003 | 0.0021 | 0.0018 | 0.0009 | 0.0078 | 0.0006 | 0.0034 | 0.0022 | 0.0001 | 0.0072 | 0.0001 | 0.0206 | 0.0007 |
Nucleolus | 0.016 | 0.0416 | 0.0191 | 0.0129 | 0.0288 | 0.0018 | 0.0358 | 0.0271 | 0.0101 | 0.0088 | 0.0726 | 0.0081 | 0.025 | 0.0451 | 0.0076 | 0.0005 | 0.0115 | 0.0058 |
Nucleus | 0.0021 | 0.0022 | 0.0009 | 0.0004 | 0.0005 | 0.0002 | 0.002 | 0.0007 | 0.0005 | 0.0075 | 0.0017 | 0.0004 | 0.0017 | 0.0016 | 0.0011 | 0 | 0.0095 | 0.0003 |
Peroxisomes | 0.0443 | 0.0432 | 0.113 | 0.0708 | 0.047 | 0.0492 | 0.0385 | 0.041 | 0.0697 | 0.0615 | 0.0275 | 0.0273 | 0.0478 | 0.0432 | 0.0481 | 0.0648 | 0.025 | 0.0176 |
Punctate Nuclear | 0.0385 | 0.0244 | 0.0174 | 0.0251 | 0.0018 | 0.0137 | 0.0222 | 0.0194 | 0.0268 | 0.0093 | 0.1347 | 0.0124 | 0.0504 | 0.0082 | 0.0209 | 0.0012 | 0.0137 | 0.0066 |
Vacuole | 0.006 | 0.0113 | 0.0027 | 0.0013 | 0.0009 | 0.0024 | 0.0108 | 0.0014 | 0.0014 | 0.0044 | 0.0037 | 0.0014 | 0.0012 | 0.0036 | 0.0004 | 0.0003 | 0.0099 | 0.0004 |
Vacuole Periphery | 0.0021 | 0.0062 | 0.0019 | 0.0012 | 0.0005 | 0.001 | 0.0061 | 0.0037 | 0.0016 | 0.0018 | 0.0022 | 0.0037 | 0.0013 | 0.0026 | 0.0008 | 0.0004 | 0.0048 | 0.0008 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 12.5842 | 10.2268 | 11.7625 | 18.7573 | 14.091 | 13.1489 | 17.7078 | 23.3797 | 19.1257 | 21.9262 |
Translational Efficiency | 0.644 | 1.0246 | 0.8149 | 0.5249 | 0.5835 | 0.6027 | 0.6882 | 0.4521 | 0.6555 | 0.5609 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1961 | 1518 | 832 | 1019 | 1662 | 2511 | 247 | 212 | 3623 | 4029 | 1079 | 1231 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 742.99 | 878.75 | 1106.80 | 1069.98 | 829.26 | 900.76 | 1211.07 | 1088.34 | 782.57 | 892.47 | 1130.67 | 1073.14 |
Standard Deviation | 115.35 | 161.85 | 172.39 | 172.84 | 126.60 | 148.68 | 172.87 | 183.52 | 128.07 | 154.14 | 177.98 | 174.87 |
Intensity Change Log 2 | — | 0.242110 | 0.574980 | 0.526169 | — | 0.119318 | 0.546386 | 0.392233 | — | 0.178649 | 0.559969 | 0.457078 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001601 | 0.004533 | 0.002528 | 0.050429 | 0.001356 | 0.001359 | 0.012467 | 0.069893 | 0.001489 | 0.002555 | 0.004803 | 0.053781 |
Bud Neck | 0.002652 | 0.002138 | 0.013478 | 0.005163 | 0.001576 | 0.003360 | 0.008558 | 0.005991 | 0.002158 | 0.002899 | 0.012352 | 0.005306 |
Bud Site | 0.001535 | 0.001054 | 0.004205 | 0.004654 | 0.000577 | 0.001463 | 0.003860 | 0.004608 | 0.001095 | 0.001309 | 0.004127 | 0.004646 |
Cell Periphery | 0.000142 | 0.000090 | 0.000103 | 0.000135 | 0.000060 | 0.000117 | 0.000104 | 0.000135 | 0.000104 | 0.000107 | 0.000103 | 0.000135 |
Cytoplasm | 0.000988 | 0.001643 | 0.001308 | 0.002221 | 0.000883 | 0.001480 | 0.001207 | 0.002277 | 0.000940 | 0.001541 | 0.001285 | 0.002231 |
Cytoplasmic Foci | 0.072706 | 0.121992 | 0.175391 | 0.233707* | 0.091498 | 0.138889 | 0.212073* | 0.173790 | 0.081327 | 0.132523 | 0.183788* | 0.223388* |
Eisosomes | 0.000175 | 0.000617 | 0.000666 | 0.000261 | 0.000507 | 0.000241 | 0.000345 | 0.000423 | 0.000327 | 0.000382 | 0.000593 | 0.000289 |
Endoplasmic Reticulum | 0.000900 | 0.000843 | 0.000775 | 0.000754 | 0.000730 | 0.000597 | 0.000906 | 0.000555 | 0.000822 | 0.000690 | 0.000805 | 0.000720 |
Endosome | 0.038814 | 0.035269 | 0.018701 | 0.116091 | 0.013543 | 0.065264 | 0.044978 | 0.124457 | 0.027221 | 0.053963 | 0.024717 | 0.117532 |
Golgi | 0.038563 | 0.103156 | 0.020901 | 0.125290* | 0.058648 | 0.059095 | 0.053591 | 0.164855* | 0.047777 | 0.075696 | 0.028384 | 0.132104* |
Lipid Particles | 0.705328* | 0.576458* | 0.384137* | 0.233083* | 0.713484* | 0.538330* | 0.427824* | 0.151241* | 0.709069* | 0.552696* | 0.394138* | 0.218989* |
Mitochondria | 0.036249 | 0.011217 | 0.005756 | 0.036126 | 0.005218 | 0.020511 | 0.002495 | 0.050333 | 0.022014 | 0.017009 | 0.005009 | 0.038573 |
Mitotic Spindle | 0.017076 | 0.017184 | 0.205204* | 0.054634 | 0.018640 | 0.037773 | 0.124825* | 0.029302 | 0.017794 | 0.030016 | 0.186804* | 0.050271 |
None | 0.000695 | 0.000438 | 0.000143 | 0.000110 | 0.000254 | 0.000627 | 0.000066 | 0.000119 | 0.000493 | 0.000556 | 0.000125 | 0.000112 |
Nuclear Periphery | 0.002211 | 0.000967 | 0.009896 | 0.002849 | 0.000976 | 0.001937 | 0.004768 | 0.001017 | 0.001644 | 0.001572 | 0.008722 | 0.002534 |
Nuclear Periphery Foci | 0.025652 | 0.012258 | 0.056007 | 0.007198 | 0.018831 | 0.028418 | 0.023561 | 0.001545 | 0.022523 | 0.022329 | 0.048580 | 0.006224 |
Nucleolus | 0.005129 | 0.003494 | 0.009671 | 0.000339 | 0.002683 | 0.003278 | 0.000953 | 0.000080 | 0.004007 | 0.003359 | 0.007675 | 0.000294 |
Nucleus | 0.001945 | 0.000670 | 0.001130 | 0.000160 | 0.000434 | 0.000697 | 0.000236 | 0.000051 | 0.001252 | 0.000687 | 0.000926 | 0.000142 |
Peroxisomes | 0.042986 | 0.103279* | 0.080420 | 0.109708* | 0.068363 | 0.091837 | 0.067409 | 0.210573* | 0.054628 | 0.096148 | 0.077441 | 0.127079* |
Vacuole | 0.003309 | 0.002163 | 0.008505 | 0.013120 | 0.001484 | 0.003656 | 0.008948 | 0.006657 | 0.002472 | 0.003093 | 0.008606 | 0.012007 |
Vacuole Periphery | 0.001346 | 0.000538 | 0.001074 | 0.003966 | 0.000255 | 0.001071 | 0.000824 | 0.002099 | 0.000845 | 0.000870 | 0.001017 | 0.003644 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.81 | -1.59 | -11.55 | -10.72 | -11.28 | 0.01 | -4.19 | -6.67 | -6.67 | -5.43 | -3.18 | -4.42 | -13.34 | -13.04 | -12.30 |
Bud Neck | 1.06 | -11.67 | -3.52 | -4.87 | 8.10 | -5.63 | -5.46 | -4.37 | -2.58 | 1.62 | -2.55 | -13.66 | -5.49 | -4.43 | 8.01 |
Bud Site | 1.01 | -5.00 | -4.49 | -5.88 | -0.80 | -5.05 | -4.55 | -5.71 | -4.37 | -0.64 | -0.94 | -7.69 | -6.41 | -6.39 | -0.99 |
Cell Periphery | 3.04 | 2.56 | 0.30 | -4.28 | -4.26 | -3.03 | -4.81 | -6.41 | -0.79 | -2.04 | -0.19 | 0.04 | -3.18 | -2.21 | -4.66 |
Cytoplasm | -2.55 | -1.63 | -5.55 | -2.30 | -6.14 | -2.75 | -1.50 | -6.96 | -2.76 | -2.16 | -3.41 | -2.79 | -8.21 | -3.57 | -6.08 |
Cytoplasmic Foci | -12.81 | -17.09 | -25.52 | -16.47 | -6.96 | -12.92 | -11.04 | -6.88 | -2.89 | 2.39 | -19.37 | -19.53 | -25.41 | -15.75 | -5.30 |
Eisosomes | -11.06 | -10.62 | -3.86 | 8.34 | 8.31 | 7.98 | 3.27 | 1.50 | -3.60 | -1.25 | -2.45 | -6.68 | 1.68 | 4.00 | 7.55 |
Endoplasmic Reticulum | 0.47 | 1.42 | 1.67 | 1.05 | 0.32 | 2.15 | -1.54 | 2.16 | 0.68 | 2.95 | 2.17 | 0.30 | 1.76 | -0.40 | 1.54 |
Endosome | 1.12 | 7.46 | -15.56 | -16.19 | -20.50 | -18.92 | -6.00 | -10.95 | -5.71 | -7.04 | -12.06 | 1.17 | -21.11 | -14.23 | -20.90 |
Golgi | -17.65 | 8.60 | -17.00 | -3.75 | -20.98* | -0.15 | 1.02 | -8.42 | -8.42 | -8.33 | -13.07 | 9.61 | -17.79 | -11.62 | -21.61 |
Lipid Particles | 12.45 | 25.21 | 45.14* | 30.94* | 10.96 | 19.34* | 14.05 | 32.71 | 22.52 | 10.83 | 22.86 | 29.88 | 57.05 | 38.28 | 14.40 |
Mitochondria | 8.31 | 10.43 | 0.03 | -6.27 | -7.79 | -9.02 | 2.79 | -4.80 | -3.15 | -5.10 | 2.68 | 9.73 | -4.33 | -5.90 | -9.33 |
Mitotic Spindle | -0.01 | -17.76 | -7.63 | -7.46 | 13.09 | -6.00 | -6.55 | -1.32 | 1.18 | 5.44 | -5.66 | -18.75 | -7.62 | -4.54 | 13.89 |
None | 1.51 | 3.92 | 4.13 | 3.25 | 3.36 | -1.46 | 3.37 | 2.36 | 2.05 | -2.47 | -0.35 | 4.57 | 4.72 | 2.78 | 1.54 |
Nuclear Periphery | 5.69 | -8.79 | -1.14 | -3.43 | 6.96 | -7.15 | -5.52 | -0.14 | 2.83 | 5.01 | 0.51 | -10.29 | -1.94 | -2.11 | 7.58 |
Nuclear Periphery Foci | 5.34 | -6.14 | 7.79 | 2.86 | 11.00 | -3.53 | -0.79 | 9.46 | 14.39 | 4.23 | 0.37 | -6.65 | 9.84 | 10.41 | 11.57 |
Nucleolus | 1.49 | -2.76 | 5.65 | 4.50 | 6.33 | -0.88 | 2.47 | 4.65 | 6.96 | 3.15 | 0.95 | -3.02 | 7.01 | 7.76 | 6.47 |
Nucleus | 2.24 | 1.33 | 3.26 | 3.52 | 3.76 | -1.56 | 0.63 | 4.51 | 4.37 | 2.24 | 1.76 | 0.82 | 3.69 | 4.86 | 4.00 |
Peroxisomes | -11.36 | -6.83 | -10.01 | -0.78 | -3.65 | -4.59 | 0.12 | -7.55 | -6.33 | -6.99 | -11.74 | -4.73 | -11.13 | -4.57 | -6.59 |
Vacuole | 2.94 | -8.97 | -7.14 | -8.07 | -3.23 | -8.64 | -3.47 | -3.10 | -1.78 | 1.26 | -2.65 | -9.05 | -8.20 | -7.69 | -2.30 |
Vacuole Periphery | 4.54 | 1.16 | -4.42 | -5.94 | -4.86 | -5.62 | -5.26 | -2.59 | -1.42 | -1.78 | -0.20 | -1.13 | -5.63 | -5.57 | -5.19 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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