Standard name
Human Ortholog
Description Low-affinity Fe(II) transporter of the plasma membrane
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 – – – 0 0 0.09 0.1 0.06 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0.05 0.08 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.08 0.09 – – – 0 0 0 0.09 0.07 0
Cytoplasm 0.99 0.95 0.94 0.96 0.96 0.92 0.87 0.85 0.71 0.74 0.69 0.57 0.88 0.88 0.83 – – – 0.72 0.74 0.44 0.34 0.48 0.47
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0.06 0.05 0 0.05
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.13 0.22 0.25 0.42 0.43 0.49 0.54 0 0 0 – – – 0 0.05 0.14 0.13 0.08 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0.09 0.1 0.09 0.14 0.16 0.22
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 1 2 1 0 0 0 2 0 1 0 0 31 30 51 – – – 9 3 25 21 19 13
Bud 2 1 2 4 0 10 3 7 8 16 15 8 20 20 15 – – – 4 1 7 8 9 13
Bud Neck 0 0 2 0 1 0 3 6 2 4 1 1 14 13 12 – – – 12 3 12 17 12 11
Bud Site 0 1 1 0 0 0 0 0 1 2 0 1 2 3 6 – – – – – – – – –
Cell Periphery 1 2 5 3 3 4 1 3 4 1 4 0 58 58 70 – – – 3 1 9 19 22 13
Cytoplasm 288 121 256 175 275 347 412 414 351 397 272 241 679 679 634 – – – 215 103 119 74 160 172
Endoplasmic Reticulum 0 2 2 1 0 3 1 0 1 0 0 2 12 11 17 – – – 5 0 16 11 12 18
Endosome 0 0 0 0 2 2 3 5 2 3 0 1 1 1 1 – – – 3 1 4 1 2 9
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 1 1 1 3
Mitochondria 3 5 9 7 8 49 104 123 207 231 195 229 0 0 1 – – – 12 7 37 27 26 19
Nucleus 0 0 0 1 1 1 4 2 2 2 1 3 6 6 4 – – – 0 0 0 0 0 1
Nuclear Periphery 0 1 2 0 0 0 0 0 0 0 0 2 0 0 0 – – – 0 0 0 0 0 1
Nucleolus 0 0 1 0 0 0 0 0 1 1 0 0 0 0 0 – – – 0 0 0 0 1 0
Peroxisomes 0 0 0 1 1 0 0 1 2 0 1 4 0 0 0 – – – 0 0 1 0 0 0
SpindlePole 0 0 0 0 1 1 7 4 6 9 15 10 11 10 17 – – – 0 0 1 1 3 3
Vac/Vac Membrane 1 0 1 2 5 7 11 4 3 7 6 7 3 4 15 – – – 28 13 23 30 51 78
Unique Cell Count 292 128 271 183 286 378 474 488 494 535 394 422 771 769 760 300 140 271 220 332 365
Labelled Cell Count 298 134 284 195 297 424 549 571 590 674 510 509 837 835 843 300 140 271 220 332 365


Cell Periphery, Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 3.6 3.7 4.2 3.9 3.2 3.0 3.1 2.7 2.5 2.6 2.6 5.6 5.6 6.8 3.6 4.2 4.8 5.7 5.8 6.2
Std Deviation (1e-4) 0.7 0.6 1.3 1.9 1.6 1.4 1.4 1.2 1.0 1.2 1.3 0.6 1.4 1.4 2.5 0.5 0.8 1.2 1.9 1.7 2.4
Intensity Change (Log2) – – – 0.21 0.11 -0.21 -0.29 -0.23 -0.42 -0.54 -0.47 -0.52 0.61 0.61 0.88 -0.03 0.19 0.38 0.64 0.66 0.75


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.818 0.0395 0.4212 -0.2335 0.6888 0.4531 0.6104 0.5149 0.7015 0.0942 0.4144 0.5455 -0.8526 0.3151 0.3064 0.327 0.1195 0.0422
Actin 0.0293 0.0026 0.0107 0.0007 0.0623 0.0103 0.0046 0.0008 0.0062 0.001 0.0031 0.0034 0.0002 0.0002 0.0002 0.001 0 0
Bud 0.0006 0.0005 0.0017 0.0006 0.006 0.0016 0.0003 0.0001 0.0069 0.003 0.0002 0.0006 0 0.0002 0 0.001 0 0
Bud Neck 0.0051 0.0002 0.0005 0.0008 0.002 0.0161 0.001 0.0003 0.0018 0.0003 0.0015 0.0021 0 0.0005 0.0001 0.0089 0 0.0008
Bud Periphery 0.0009 0.0017 0.0025 0.0015 0.0237 0.0068 0.0004 0.0005 0.0131 0.0103 0.0011 0.001 0 0.0001 0 0.0066 0 0
Bud Site 0.0125 0.0029 0.0034 0.0008 0.0031 0.0007 0.0041 0.0016 0.0099 0.0023 0.0003 0.0022 0.0001 0.0075 0.0001 0.0037 0 0
Cell Periphery 0.0041 0.0008 0.0007 0.0008 0.0064 0.0004 0.0012 0.0104 0.0082 0.002 0.0253 0.0004 0.0003 0.0002 0.0002 0.0246 0 0.0002
Cytoplasm 0.0805 0.0979 0.0992 0.1205 0.073 0.0925 0.1203 0.3203 0.2097 0.3218 0.4126 0.2939 0.0425 0.03 0.0949 0.3081 0.0237 0.1839
Cytoplasmic Foci 0.0585 0.0063 0.0092 0.0276 0.0356 0.0128 0.0336 0.0108 0.0166 0.0075 0.0172 0.0166 0.0116 0.0021 0.0015 0.0083 0.0008 0.0025
Eisosomes 0.0004 0.0003 0.0005 0.0002 0.0006 0.0002 0.0003 0.0002 0.0005 0.0001 0.0003 0.0001 0.0005 0.0005 0.0002 0.0003 0.0001 0.0001
Endoplasmic Reticulum 0.0081 0.0008 0.0016 0.0015 0.0048 0.0015 0.0053 0.0053 0.0028 0.0031 0.0028 0.0017 0.0015 0.0007 0.0012 0.0027 0.0001 0.0005
Endosome 0.0486 0.0024 0.0028 0.0049 0.0551 0.0194 0.0255 0.0035 0.0102 0.0576 0.0063 0.0139 0.0017 0.0019 0.0012 0.0014 0 0.0011
Golgi 0.0117 0.0002 0.0014 0.0004 0.0273 0.0072 0.0037 0.0003 0.0027 0.0161 0.001 0.0035 0.0002 0.0001 0.0001 0.0002 0 0
Lipid Particles 0.0296 0.0005 0.0042 0.0026 0.0431 0.0113 0.0538 0.0027 0.0067 0.0028 0.0013 0.0026 0.0046 0.0002 0.0001 0.0089 0 0.0001
Mitochondria 0.0038 0.0002 0.001 0.0011 0.0472 0.002 0.001 0.0001 0.0025 0.0015 0.0002 0.0032 0.0003 0.0002 0.0001 0.0002 0 0
None 0.6418 0.8789 0.8081 0.8131 0.5725 0.7922 0.7078 0.6352 0.6862 0.5641 0.5182 0.6327 0.9334 0.8947 0.868 0.6168 0.9752 0.7883
Nuclear Periphery 0.0061 0.0002 0.0053 0.0009 0.0042 0.0038 0.0042 0.0004 0.0005 0.0009 0.0002 0.0005 0.0005 0.0003 0.0003 0.0003 0 0.0001
Nucleolus 0.003 0.0002 0.0003 0.0016 0.0008 0.0007 0.0027 0.0001 0.0006 0.0003 0.0001 0.0003 0.0003 0.0004 0.0001 0.0004 0 0
Nucleus 0.0111 0.0005 0.0073 0.0016 0.0028 0.0006 0.0026 0.0006 0.0008 0.0009 0.0004 0.0009 0.0004 0.0017 0.0004 0.0008 0 0.0002
Peroxisomes 0.0162 0.0001 0.0289 0.0009 0.0069 0.0028 0.0024 0 0.0016 0.0005 0.0001 0.0042 0.0002 0 0 0.0002 0 0
Punctate Nuclear 0.0203 0.0003 0.0008 0.0032 0.0034 0.0022 0.0154 0.0002 0.0005 0.0005 0.0002 0.0018 0.0006 0.0034 0.0001 0.0005 0 0
Vacuole 0.0065 0.0027 0.009 0.0126 0.0179 0.0131 0.009 0.0063 0.0114 0.0026 0.007 0.0144 0.0012 0.0494 0.031 0.0051 0 0.0202
Vacuole Periphery 0.0014 0.0001 0.0008 0.0022 0.0015 0.0019 0.0009 0.0001 0.0005 0.0008 0.0004 0.0003 0.0001 0.0056 0.0003 0.0001 0 0.002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.6158 28.5389 19.8535 20.5851 17.2467 31.2425 42.9829 37.3283 36.4286 45.7914
Translational Efficiency 0.6636 0.9801 0.8666 0.8275 0.6909 0.523 0.7963 0.8459 0.7405 0.7635

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
116 443 1220 1324 93 1330 1716 1496 209 1773 2936 2820

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 899.85 872.80 2072.90 854.50 700.41 731.65 977.00 820.22 811.10 766.92 1432.38 836.31
Standard Deviation 106.27 148.28 461.12 114.19 90.92 100.53 189.37 124.75 140.61 129.65 633.24 121.12
Intensity Change Log 2 — -0.044033 1.203894 -0.074604 — 0.062954 0.480159 0.227811 — 0.003773 0.930456 0.065614

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000685 0.007028 0.000682 0.011514 0.003579 0.002176 0.041094 0.006627 0.001973 0.003388 0.024301 0.008921
Bud Neck 0.009395 0.105078 0.003310 0.024872 0.137528 0.099240 0.097472 0.027142 0.066411 0.100698 0.058345 0.026076
Bud Site 0.020728 0.078507 0.009895 0.096625 0.095197 0.037820 0.287654* 0.090708 0.053865 0.047986 0.172237* 0.093486
Cell Periphery 0.000370 0.080003 0.897115* 0.018137 0.026064 0.007973 0.207169* 0.008461 0.011803 0.025970 0.493863* 0.013004
Cytoplasm 0.225727* 0.250512* 0.001837 0.394882* 0.306500* 0.358176* 0.213125* 0.435784* 0.261669* 0.331275* 0.125328* 0.416581*
Cytoplasmic Foci 0.072466 0.080662 0.000087 0.032229 0.224577* 0.197530* 0.009153 0.036721 0.140152 0.168330 0.005386 0.034612
Eisosomes 0.000178 0.003541 0.002028 0.003685 0.004005 0.002710 0.008586 0.001872 0.001881 0.002917 0.005861 0.002724
Endoplasmic Reticulum 0.002638 0.010645 0.003308 0.005658 0.000565 0.001847 0.016006 0.002737 0.001716 0.004045 0.010730 0.004108
Endosome 0.030864 0.027206 0.000433 0.004345 0.019200 0.040071 0.002206 0.007458 0.025674 0.036856 0.001469 0.005996
Golgi 0.003930 0.004764 0.000585 0.001677 0.004583 0.010995 0.000760 0.001925 0.004220 0.009438 0.000687 0.001809
Lipid Particles 0.003986 0.005364 0.002397 0.003205 0.005771 0.012575 0.006904 0.002148 0.004780 0.010773 0.005032 0.002644
Mitochondria 0.019929 0.002158 0.000214 0.001308 0.001703 0.010249 0.000724 0.002896 0.011819 0.008228 0.000512 0.002150
Mitotic Spindle 0.003556 0.000101 0.000509 0.011608 0.000733 0.001255 0.000329 0.019235 0.002300 0.000966 0.000404 0.015654
None 0.000412 0.000148 0.000016 0.001077 0.000263 0.000977 0.000313 0.003201 0.000346 0.000770 0.000190 0.002203
Nuclear Periphery 0.001091 0.001275 0.002026 0.000453 0.000136 0.000211 0.000114 0.000545 0.000666 0.000477 0.000909 0.000502
Nuclear Periphery Foci 0.001799 0.001600 0.000304 0.001762 0.000202 0.000246 0.000998 0.000699 0.001088 0.000584 0.000709 0.001198
Nucleolus 0.000502 0.000235 0.000068 0.000126 0.000572 0.000537 0.000049 0.000599 0.000534 0.000462 0.000057 0.000377
Nucleus 0.118406 0.021777 0.000203 0.139112* 0.050952 0.048461 0.035996 0.148710* 0.088391 0.041794 0.021123 0.144204*
Peroxisomes 0.002975 0.008271 0.000065 0.001322 0.043899 0.021780 0.001310 0.002601 0.021185 0.018404 0.000793 0.002000
Vacuole 0.474516* 0.309584* 0.072865 0.244732* 0.073476 0.142729 0.069845 0.198482 0.296063* 0.184419 0.071100 0.220196*
Vacuole Periphery 0.005847 0.001541 0.002052 0.001670 0.000494 0.002443 0.000192 0.001450 0.003465 0.002217 0.000965 0.001554

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.86 -0.70 -7.94 -0.02 -7.82 0.96 -14.99 -0.45 -2.72 16.73* -1.98 -15.77 -4.55 -3.05 13.48
Bud Neck -12.77 1.56 -4.57 11.60 -9.85 1.60 1.86 5.40 17.02 18.33 -3.37 0.64 4.30 20.93 14.88
Bud Site -6.02 1.40 -5.51 1.83 -11.04 2.69 -8.79 1.62 -4.08 27.30* 0.40 -10.50 -0.83 -3.04 18.92*
Cell Periphery -8.61 -195.98* -6.46 7.40 183.42* 1.92 -15.44 2.25 1.90 28.49* -2.98 -54.76 0.92 6.68 61.93*
Cytoplasm -0.89 10.04 -5.53 -8.33 -49.30* -2.01 3.12 -3.41 -3.87 -19.50 -3.77 7.50 -6.48 -6.11 -39.09
Cytoplasmic Foci -3.39 8.60 3.88 7.87 -20.74 1.11 8.92 8.03 29.40 -11.88 -2.97 9.66 7.78 29.64 -20.30
Eisosomes -3.51 -6.98 -7.22 1.34 -0.34 0.68 -2.38 1.55 4.69 11.42 -1.29 -4.47 0.24 3.72 10.08
Endoplasmic Reticulum -3.15 -0.53 -3.07 2.39 -2.06 -4.54 -10.86 -5.35 -1.78 9.21 -3.10 -9.67 -4.55 0.55 7.85
Endosome -1.83 3.73 3.08 9.12 -6.21 -3.97 3.84 3.16 16.04 -4.08 -4.96 5.25 4.33 18.80 -6.30
Golgi -1.66 0.91 0.64 4.69 -1.27 -3.46 2.49 2.16 8.54 -1.19 -4.63 2.25 1.84 9.51 -1.68
Lipid Particles -2.47 0.77 0.97 4.20 -0.29 -5.18 -0.70 4.19 11.54 5.02 -7.22 -0.62 3.93 11.92 3.80
Mitochondria 1.00 1.26 1.17 1.66 -2.77 -6.38 2.62 -0.27 6.18 -2.62 -0.47 1.35 1.13 6.70 -3.48
Mitotic Spindle 2.62 2.28 -2.90 -4.97 -4.76 -0.64 0.54 -6.10 -6.03 -6.48 1.46 2.31 -6.34 -7.56 -8.04
None 2.73 4.07 -3.76 -5.83 -6.59 -1.98 -0.22 -5.40 -3.25 -5.43 -1.46 2.30 -6.38 -3.73 -7.35
Nuclear Periphery -0.04 -2.93 1.37 0.33 7.52 -1.51 0.17 -15.19 -14.44 -17.28 0.86 -1.22 -1.69 -1.78 -0.75
Nuclear Periphery Foci 0.14 5.50 0.04 -0.14 -12.11 -0.37 -7.41 -6.66 -9.76 2.62 1.64 1.90 -0.99 -2.52 -5.84
Nucleolus 1.07 3.00 2.00 2.70 -7.97 0.40 1.63 0.48 0.28 -3.09 0.19 2.40 0.82 1.44 -3.64
Nucleus 5.56 6.93 -2.23 -24.41 -32.31 0.59 1.02 -13.04 -22.80 -22.90 4.55 6.06 -7.11 -31.46 -36.45
Peroxisomes -4.39 3.26 1.46 5.13 -3.94 1.66 3.26 3.23 11.15 -1.01 0.30 3.29 3.20 12.24 -2.36
Vacuole 6.67 15.32 6.65 -0.88 -29.35 -3.89 -0.09 -11.54 -14.31 -23.76 6.02 10.67 1.41 -14.51 -37.22
Vacuole Periphery 0.70 0.44 0.95 1.16 2.57 -5.49 3.45 -1.12 3.23 -2.08 -0.32 0.80 0.80 3.44 0.08
Standard name
Human Ortholog
Description Low-affinity Fe(II) transporter of the plasma membrane
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Fet4

Fet4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fet4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available