Standard name
Human Ortholog
Description Nucleoside diphosphate kinase; catalyzes the transfer of gamma phosphates from nucleoside triphosphates, usually ATP, to nucleoside diphosphates by a mechanism that involves formation of an autophosphorylated enzyme intermediate; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.98 0.97 0.95 0.93 0.87 0.85 0.78 0.74 0.77 0.73 0.99 0.99 0.99 0.95 0.95 0.96 0.96 0.9 0.96 0.92 0.95 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.05 0.05 0.08 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.09 0 0.09 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.05 0.06 0.05 0.08 0.11 0.09 0.11 0 0 0 0.06 0.06 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 4 6 2 6 2 7 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 4 0 1 1 0 0 1 0 1 3 0 0 0 0 0 0 2 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 5 2 2 1 0 2 7 10 14 17 13 17 1 0 0 3 3 6 0 0 0 0 0 0
Cytoplasm 493 149 228 203 296 338 430 391 300 325 203 330 241 260 305 257 161 184 477 132 222 85 205 171
Endoplasmic Reticulum 0 0 1 1 6 6 18 22 20 33 12 24 1 2 3 8 3 8 1 0 0 0 1 1
Endosome 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 1 0
Golgi 1 0 0 0 0 0 1 2 3 1 2 1 0 0 1 0 0 1 0 0 0 0 0 0
Mitochondria 0 0 1 0 2 6 13 5 33 19 23 31 0 0 1 4 2 0 1 2 1 0 1 0
Nucleus 1 0 2 0 0 1 1 6 2 6 2 9 0 0 1 1 0 1 5 2 2 2 2 4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 0 0 0 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Vac/Vac Membrane 6 0 2 4 13 17 32 24 30 49 23 51 1 0 0 16 10 12 5 2 1 2 2 2
Unique Cell Count 501 150 232 209 312 365 494 458 386 439 263 450 243 262 309 271 169 191 500 146 233 93 217 186
Labelled Cell Count 506 152 240 210 318 372 506 466 405 459 282 476 244 262 311 289 179 213 500 146 233 93 217 186


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 20.5 27.5 12.9 16.2 20.8 19.3 20.3 20.7 20.9 21.7 22.1 20.5 21.8 24.4 25.6 31.1 28.2 31.6 19.9 21.1 20.9
Std Deviation (1e-4) 4.3 7.7 3.3 3.6 4.9 4.1 5.0 5.3 5.8 6.2 6.3 7.2 5.9 6.7 6.8 10.5 10.1 16.6 4.4 5.2 5.2
Intensity Change (Log2) – – – 0.33 0.69 0.58 0.66 0.68 0.7 0.75 0.78 0.67 0.76 0.92 0.99 1.27 1.13 1.3 0.63 0.71 0.7


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 2.1 2.2 2.6 2.2 0 0 0 0 0 0
Cytoplasm -0.8 -2.1 -3.0 -4.8 -5.3 -7.0 -7.8 -7.0 -8.0 0.9 1.0 0.4 -2.1 -1.7 -1.2
Endoplasmic Reticulum 0 0 0 2.5 3.0 3.2 4.0 2.9 3.2 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 4.3 2.8 4.3 3.8 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 2.3 2.6 3.3 2.9 3.8 4.8 4.0 4.8 0 0 0 3.0 2.9 3.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 21.4824 30.5231 29.558 25.8093 23.6601 25.6475 17.6788 25.534 23.4397 22.5383 20.4122 20.8284 20.3134 30.3108 28.8444 22.8053 26.2925 25.3901
Actin 0.0245 0.0001 0.0009 0.0001 0.0041 0.0001 0.0095 0.0002 0.0054 0.0005 0.014 0.0009 0.0132 0.0001 0.0027 0.0735 0 0.0001
Bud 0.0011 0 0.0001 0.0001 0.0005 0.0001 0.0007 0.0001 0.0016 0.0004 0.0004 0.0001 0.0005 0 0.0004 0.0018 0 0.0001
Bud Neck 0.0008 0.0001 0.0004 0.0002 0.0016 0.0005 0.0007 0.0001 0.0006 0.0004 0.0005 0.0008 0.0018 0.0001 0.0003 0.0007 0.0005 0.0006
Bud Periphery 0.0011 0 0 0 0.0004 0 0.0005 0 0.0015 0.0001 0.0006 0.0001 0.0004 0 0.0002 0.0027 0 0
Bud Site 0.0018 0 0.0003 0 0.0008 0 0.0018 0.0001 0.0052 0.0003 0.0005 0.0002 0.0015 0 0.0003 0.005 0 0
Cell Periphery 0.0002 0 0 0 0.0001 0 0.0001 0 0.0002 0 0 0 0.0002 0 0 0.0003 0 0
Cytoplasm 0.8691 0.9937 0.97 0.9658 0.8927 0.9614 0.8182 0.9591 0.9019 0.901 0.8476 0.8838 0.8144 0.9899 0.9611 0.7786 0.945 0.9671
Cytoplasmic Foci 0.0143 0.0002 0.0008 0.0003 0.0191 0.0005 0.0289 0.0009 0.0049 0.0255 0.0147 0.0059 0.0308 0.0003 0.0023 0.0118 0.0002 0.0008
Eisosomes 0.0003 0 0.0001 0 0 0 0.0001 0 0.0002 0 0.0001 0 0.0001 0 0 0.0004 0 0
Endoplasmic Reticulum 0.0031 0.0001 0.0004 0.0002 0.0038 0.0004 0.0047 0.0003 0.001 0.0035 0.0007 0.0015 0.0047 0.0002 0.0002 0.0041 0.0007 0.0009
Endosome 0.0082 0 0.0005 0.0001 0.0198 0.0003 0.0352 0.0001 0.0088 0.011 0.0249 0.0044 0.0179 0.0001 0.0006 0.0123 0.0001 0.0002
Golgi 0.0018 0 0.0001 0 0.0052 0 0.0087 0 0.0048 0.0011 0.0207 0.0023 0.0044 0 0.0003 0.0126 0 0
Lipid Particles 0.004 0 0.0002 0 0.0109 0 0.0053 0 0.0024 0.0038 0.005 0.0023 0.0117 0 0.0001 0.0107 0 0
Mitochondria 0.0061 0 0.0001 0.0001 0.0018 0.0001 0.005 0 0.0052 0.0013 0.0182 0.01 0.0051 0 0.0017 0.0489 0 0.0001
None 0.0037 0.0002 0.005 0.0002 0.0017 0.0003 0.0014 0.0003 0.001 0.0012 0.0003 0.001 0.009 0.0002 0.0003 0.0015 0.0002 0.0003
Nuclear Periphery 0.0086 0.0007 0.0013 0.0026 0.0034 0.0031 0.0134 0.0015 0.0057 0.0082 0.0036 0.0047 0.019 0.0008 0.0013 0.0061 0.004 0.0042
Nucleolus 0.0006 0 0.0003 0 0.0006 0 0.0003 0.0003 0.0006 0.0003 0.0001 0.0012 0.0006 0 0 0.0003 0 0
Nucleus 0.0364 0.0047 0.0177 0.0298 0.0088 0.0321 0.0502 0.0362 0.0427 0.0323 0.0418 0.0752 0.0447 0.008 0.0271 0.0106 0.0485 0.025
Peroxisomes 0.0008 0 0.0001 0 0.0072 0 0.0082 0 0.0027 0.0013 0.0044 0.0014 0.0077 0 0.0007 0.0074 0 0
Punctate Nuclear 0.0064 0.0001 0.0011 0.0002 0.0139 0.0007 0.002 0.0005 0.0009 0.0042 0.0007 0.003 0.0067 0.0001 0.0002 0.0079 0.0002 0.0003
Vacuole 0.0045 0 0.0002 0.0002 0.0028 0.0003 0.0036 0.0001 0.0017 0.0025 0.0008 0.001 0.0039 0.0001 0.0002 0.0013 0.0003 0.0003
Vacuole Periphery 0.0025 0 0.0001 0.0001 0.0008 0.0001 0.0016 0 0.0009 0.0009 0.0006 0.0003 0.0017 0 0.0001 0.0013 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 224.5022 155.856 72.8765 99.7749 193.5352 238.6999 164.6016 155.1688 158.191 211.6674
Translational Efficiency 3.0094 2.6508 2.2439 2.4234 2.7428 3.5499 2.982 2.0998 2.1288 2.7443

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1775 1478 1786 1256 2283 1736 2321 1534 4058 3214 4107 2790

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1026.90 1552.78 3666.60 3755.23 1363.21 1489.69 2764.45 3376.14 1216.11 1518.70 3156.77 3546.80
Standard Deviation 207.46 308.09 802.81 943.85 255.23 279.15 529.40 872.10 288.63 294.49 799.17 924.54
Intensity Change Log 2 — 0.596558 1.836147 1.870606 — 0.128004 1.019985 1.308367 — 0.348166 1.427977 1.577102

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000175 0.000298 0.000076 0.000765 0.000095 0.000217 0.000093 0.000353 0.000130 0.000254 0.000085 0.000539
Bud Neck 0.000854 0.000677 0.000887 0.004689 0.000729 0.000702 0.000824 0.004030 0.000783 0.000690 0.000851 0.004327
Bud Site 0.003323 0.003183 0.000482 0.014328 0.002103 0.004532 0.001350 0.019393 0.002637 0.003912 0.000973 0.017113
Cell Periphery 0.000082 0.000066 0.000182 0.000790 0.000038 0.000056 0.000096 0.000484 0.000057 0.000061 0.000133 0.000621
Cytoplasm 0.836285* 0.824114* 0.548299* 0.628765* 0.854831* 0.844309* 0.680909* 0.688076* 0.846719* 0.835022* 0.623241* 0.661375*
Cytoplasmic Foci 0.038507 0.013398 0.003003 0.011639 0.016888 0.020083 0.002544 0.010038 0.026344 0.017009 0.002744 0.010759
Eisosomes 0.000008 0.000003 0.000001 0.000006 0.000002 0.000004 0.000001 0.000004 0.000005 0.000003 0.000001 0.000005
Endoplasmic Reticulum 0.001356 0.000365 0.000027 0.000223 0.000351 0.000524 0.000038 0.000365 0.000791 0.000451 0.000033 0.000301
Endosome 0.001684 0.000548 0.000097 0.004387 0.000487 0.002575 0.000180 0.006556 0.001010 0.001643 0.000144 0.005580
Golgi 0.000689 0.000817 0.000194 0.007022 0.000631 0.002369 0.000167 0.005606 0.000656 0.001655 0.000179 0.006243
Lipid Particles 0.000553 0.000646 0.000180 0.001862 0.000234 0.000453 0.000213 0.001422 0.000374 0.000542 0.000199 0.001620
Mitochondria 0.001232 0.000734 0.000311 0.000733 0.000200 0.001220 0.000205 0.000980 0.000651 0.000997 0.000251 0.000869
Mitotic Spindle 0.001729 0.012021 0.001022 0.027301 0.002768 0.013545 0.004563 0.026090 0.002313 0.012844 0.003024 0.026635
None 0.012796 0.010352 0.012009 0.016269 0.006862 0.006099 0.013279 0.012316 0.009458 0.008054 0.012727 0.014095
Nuclear Periphery 0.000123 0.000125 0.000262 0.000493 0.000109 0.000181 0.000252 0.000317 0.000115 0.000155 0.000256 0.000396
Nuclear Periphery Foci 0.004222 0.002520 0.000364 0.002991 0.001491 0.003370 0.000536 0.001564 0.002685 0.002979 0.000461 0.002207
Nucleolus 0.000428 0.000189 0.000080 0.000290 0.000137 0.000393 0.000086 0.000222 0.000264 0.000299 0.000084 0.000252
Nucleus 0.073600 0.120054 0.430119* 0.251385* 0.098819 0.080288 0.288478* 0.204640* 0.087788 0.098575 0.350073* 0.225683*
Peroxisomes 0.000192 0.000177 0.000005 0.000258 0.000087 0.000426 0.000012 0.000179 0.000133 0.000311 0.000009 0.000215
Vacuole 0.021321 0.008990 0.002321 0.023293 0.012432 0.016323 0.005583 0.015817 0.016320 0.012951 0.004164 0.019182
Vacuole Periphery 0.000841 0.000725 0.000081 0.002512 0.000706 0.002334 0.000590 0.001549 0.000765 0.001594 0.000369 0.001982

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.85 2.17 -3.32 -0.97 -3.98 -3.37 -2.10 -4.84 0.47 -1.83 -3.20 -0.56 -4.68 -0.70 -4.36
Bud Neck -0.34 -3.30 -7.81 -7.91 -5.59 -0.93 -4.27 -8.92 -9.03 -5.40 -0.94 -5.37 -11.57 -11.66 -7.71
Bud Site -0.19 8.44 -4.18 -3.62 -7.17 -4.16 0.19 -4.80 -2.59 -4.86 -3.29 4.59 -6.32 -4.18 -7.88
Cell Periphery -0.32 -10.41 -13.33 -13.25 -6.96 -3.44 -12.42 -9.85 -9.23 -4.89 -2.94 -15.56 -16.41 -15.93 -8.52
Cytoplasm 4.66 49.67 33.61 28.37 -9.13 4.69 44.03 32.43 25.21 -3.96 6.71 64.89 46.48 37.46 -8.67
Cytoplasmic Foci 10.96 17.03 10.81 -0.29 -8.45 -2.09 10.91 3.89 5.30 -7.40 6.44 19.95 10.84 3.94 -11.22
Eisosomes 5.78 8.71 2.82 -3.88 -8.33 -3.60 0.78 -2.86 1.00 -6.13 2.49 7.54 0.62 -2.51 -10.29
Endoplasmic Reticulum 2.86 4.03 3.83 3.05 -3.12 -1.60 6.28 4.33 4.58 -1.84 2.17 5.12 4.68 5.43 -3.07
Endosome 4.64 7.03 1.71 -1.14 -2.61 -4.74 5.74 -1.94 1.03 -2.66 -2.07 8.60 -0.83 0.45 -3.56
Golgi -0.23 2.88 -1.35 -1.24 -1.90 -2.79 2.10 -1.32 0.70 -2.15 -2.66 3.13 -1.88 -0.26 -2.87
Lipid Particles -0.76 0.11 -10.35 -7.57 -10.43 -3.57 -8.10 -10.24 -8.11 -7.02 -2.07 -5.76 -14.50 -10.76 -12.41
Mitochondria 0.80 1.58 1.62 0.51 0.31 -1.39 -1.98 -1.89 1.11 -1.04 -0.42 1.38 1.20 1.20 -0.69
Mitotic Spindle -5.18 -0.51 -5.14 0.66 -5.00 -5.19 -3.08 -4.29 0.75 -2.45 -7.31 -2.93 -6.57 1.00 -4.97
None 2.14 0.89 2.26 0.45 2.58 1.45 -10.82 -3.17 -4.74 8.19 2.46 -4.06 0.55 -2.50 6.68
Nuclear Periphery -2.02 -11.19 -15.60 -14.10 -2.72 -6.07 -12.55 -14.25 -8.73 -0.81 -5.76 -16.78 -21.12 -15.95 -2.51
Nuclear Periphery Foci 2.91 10.74 4.25 1.36 -8.24 -4.27 3.18 -1.55 3.61 -4.45 -2.07 10.43 2.60 3.84 -8.73
Nucleolus 2.22 4.11 1.97 -0.81 -3.34 -1.38 0.46 -2.50 1.12 -3.68 -0.16 4.11 1.28 0.68 -4.40
Nucleus -12.87 -69.63 -33.23 -22.04 25.25 3.52 -47.42 -24.81 -26.73 17.24 -6.51 -78.69 -40.53 -34.00 28.93
Peroxisomes 0.08 7.44 3.52 1.41 -3.08 -2.54 2.80 0.97 2.83 -3.56 -2.17 6.74 3.08 3.16 -4.53
Vacuole 3.63 10.23 -14.75 -16.50 -19.45 -7.22 -5.92 -16.80 -12.57 -14.20 -3.13 1.70 -22.40 -20.25 -23.20
Vacuole Periphery 0.54 3.06 0.52 0.02 -1.47 -1.83 1.44 0.90 2.34 -0.83 -1.40 2.79 0.97 2.01 -1.64
Standard name
Human Ortholog
Description Nucleoside diphosphate kinase; catalyzes the transfer of gamma phosphates from nucleoside triphosphates, usually ATP, to nucleoside diphosphates by a mechanism that involves formation of an autophosphorylated enzyme intermediate; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Ynk1

Ynk1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ynk1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available