Standard name | |
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Human Ortholog | |
Description | Protein that facilitates ribosomal subunit dissociation; Dom34-Hbs1 complex and Rli1p have roles in dissociating inactive ribosomes to facilitate translation restart, particularly ribosomes stalled in 3' UTRs; required for RNA cleavage in no-go decay, but reports conflict on endonuclease activity; Pelota ortholog; protein abundance increases in response to DNA replication stress; DOM34 has a paralog, YCL001W-B, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 2 | 0 | 4 | 6 | 11 | 4 | 9 | 12 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud | 0 | 0 | 4 | 1 | 3 | 3 | 12 | 7 | 11 | 9 | 19 | 18 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 3 | 22 | 11 | 6 | 18 | 31 | 45 | 34 | 37 | 32 | 46 | 9 | 28 | 14 | 6 | 9 | 7 | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 174 | 129 | 314 | 278 | 334 | 375 | 439 | 505 | 317 | 327 | 280 | 327 | 322 | 444 | 543 | 124 | 137 | 122 | 166 | 140 | 309 | 237 | 386 | 350 |
Endoplasmic Reticulum | 1 | 3 | 6 | 0 | 7 | 13 | 27 | 25 | 37 | 40 | 15 | 25 | 2 | 6 | 5 | 6 | 7 | 6 | 0 | 0 | 8 | 0 | 2 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 2 | 10 | 18 | 87 | 195 | 153 | 120 | 158 | 133 | 158 | 0 | 0 | 1 | 2 | 3 | 0 | 1 | 0 | 1 | 0 | 1 | 2 |
Nucleus | 1 | 0 | 0 | 0 | 1 | 2 | 3 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 2 | 0 | 1 | 0 | 1 | 0 | 0 | 2 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 0 | 0 | 1 | 8 | 9 | 4 | 23 | 40 | 17 | 16 | 23 | 14 | 0 | 0 | 0 | 1 | 2 | 7 | 0 | 1 | 0 | 2 | 4 | 1 |
Unique Cell Count | 175 | 130 | 324 | 292 | 361 | 436 | 621 | 691 | 460 | 497 | 418 | 493 | 324 | 449 | 546 | 129 | 142 | 129 | 172 | 145 | 330 | 247 | 401 | 362 |
Labelled Cell Count | 178 | 136 | 349 | 310 | 379 | 507 | 741 | 795 | 540 | 599 | 515 | 601 | 335 | 479 | 567 | 139 | 161 | 143 | 172 | 145 | 330 | 247 | 401 | 362 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 12.0 | 17.0 | 14.0 | 12.2 | 10.9 | 9.4 | 8.9 | 9.2 | 8.7 | 8.4 | 8.2 | 8.2 | 15.5 | 16.0 | 15.4 | 17.7 | 17.4 | 19.7 | 14.4 | 14.8 | 15.8 |
Std Deviation (1e-4) | 1.9 | 2.1 | 1.7 | 1.6 | 1.8 | 1.5 | 1.7 | 1.8 | 1.8 | 1.8 | 1.7 | 1.7 | 2.7 | 2.5 | 2.2 | 5.0 | 4.7 | 4.1 | 2.4 | 2.7 | 2.8 |
Intensity Change (Log2) | – | – | – | -0.2 | -0.36 | -0.58 | -0.65 | -0.61 | -0.69 | -0.73 | -0.78 | -0.77 | 0.15 | 0.2 | 0.14 | 0.34 | 0.31 | 0.49 | 0.04 | 0.08 | 0.17 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0.8 | 0 | 1.2 | 0.6 | 2.6 | 2.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.7 | -3.4 | -1.6 | -1.1 | -0.2 | 0.3 | 0.4 | 0.5 | 1.3 | -2.4 | -0.3 | -3.0 | -0.9 | -0.2 | -0.5 |
Cytoplasm | -1.1 | -2.5 | -5.1 | -9.5 | -9.0 | -9.7 | -10.5 | -10.1 | -10.4 | 2.3 | 2.0 | 3.0 | -0.4 | -0.2 | -1.2 |
Endoplasmic Reticulum | 0 | 0.1 | 1.0 | 2.0 | 1.5 | 3.7 | 3.8 | 1.4 | 2.4 | 0 | -0.6 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2.5 | 3.4 | 8.2 | 11.1 | 8.9 | 9.7 | 11.0 | 10.9 | 11.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 3.1 | 4.1 | 3.1 | 2.9 | 4.0 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 11.0065 | 13.5474 | 11.9633 | 10.9782 | 11.3637 | 11.9796 | 9.3789 | 12.9115 | 12.1728 | 10.6985 | 11.038 | 11.7975 | 9.0515 | 11.9813 | 10.3185 | 10.4525 | 8.4739 | 10.8888 |
Actin | 0.0002 | 0.0001 | 0.0007 | 0.0002 | 0.0001 | 0.0004 | 0.0058 | 0.0002 | 0.0101 | 0.0066 | 0.0007 | 0.0002 | 0.0348 | 0.0009 | 0.0031 | 0.0176 | 0.0297 | 0.0104 |
Bud | 0.0001 | 0.0001 | 0.0013 | 0.0002 | 0.0001 | 0.0001 | 0.0007 | 0.0001 | 0.0003 | 0.0007 | 0.0007 | 0.0001 | 0.0005 | 0.0003 | 0.0004 | 0.0005 | 0.0004 | 0.0001 |
Bud Neck | 0.0002 | 0.0001 | 0.0002 | 0.0004 | 0.0006 | 0.0006 | 0.0009 | 0.0001 | 0.0004 | 0.0003 | 0.0004 | 0.0008 | 0.0007 | 0.0001 | 0.0007 | 0.0004 | 0.0005 | 0.0004 |
Bud Periphery | 0 | 0 | 0.0006 | 0 | 0 | 0.0001 | 0.0009 | 0 | 0.0002 | 0.0008 | 0.0015 | 0.0001 | 0.0006 | 0.0001 | 0.0004 | 0.0001 | 0.0005 | 0.0001 |
Bud Site | 0.0002 | 0.0002 | 0.0017 | 0.0001 | 0 | 0 | 0.0008 | 0.0001 | 0.0005 | 0.0027 | 0.0006 | 0.0001 | 0.0016 | 0.001 | 0.001 | 0.0002 | 0.0012 | 0.0001 |
Cell Periphery | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0003 | 0 | 0 | 0.0012 | 0.0001 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0.0001 | 0 |
Cytoplasm | 0.9571 | 0.9886 | 0.9744 | 0.9409 | 0.9707 | 0.9758 | 0.8351 | 0.9941 | 0.9581 | 0.9267 | 0.8931 | 0.9611 | 0.8551 | 0.9806 | 0.9247 | 0.9471 | 0.7834 | 0.9563 |
Cytoplasmic Foci | 0.0131 | 0.0021 | 0.0035 | 0.0072 | 0.0046 | 0.0042 | 0.0291 | 0.0013 | 0.0049 | 0.0062 | 0.0034 | 0.0077 | 0.0113 | 0.0044 | 0.0141 | 0.012 | 0.0442 | 0.0041 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.0069 | 0.0023 | 0.0027 | 0.0044 | 0.0157 | 0.0076 | 0.0092 | 0.0008 | 0.0013 | 0.002 | 0.003 | 0.0093 | 0.0068 | 0.001 | 0.0068 | 0.0033 | 0.0017 | 0.003 |
Endosome | 0.0115 | 0.0021 | 0.0079 | 0.0356 | 0.0044 | 0.0067 | 0.0411 | 0.0012 | 0.0074 | 0.0105 | 0.0411 | 0.0097 | 0.0213 | 0.0031 | 0.0143 | 0.0075 | 0.0176 | 0.0074 |
Golgi | 0.0007 | 0.0001 | 0.0019 | 0.001 | 0.0002 | 0.0007 | 0.0074 | 0.0001 | 0.0065 | 0.0068 | 0.0026 | 0.0005 | 0.0111 | 0.0025 | 0.0018 | 0.0022 | 0.0142 | 0.0012 |
Lipid Particles | 0.0007 | 0 | 0 | 0 | 0 | 0 | 0.0076 | 0 | 0.0008 | 0.0192 | 0.0009 | 0.0001 | 0.0116 | 0.0001 | 0.0049 | 0 | 0.0224 | 0.0015 |
Mitochondria | 0.0002 | 0.0001 | 0.0007 | 0.0004 | 0.0001 | 0.0008 | 0.0233 | 0.0002 | 0.0046 | 0.0037 | 0.0198 | 0.0056 | 0.0164 | 0.0032 | 0.0047 | 0.0004 | 0.0424 | 0.0006 |
None | 0.0018 | 0.0008 | 0.0008 | 0.0006 | 0.0006 | 0.0006 | 0.0059 | 0.0004 | 0.0005 | 0.0006 | 0.0008 | 0.0006 | 0.0026 | 0.0005 | 0.0048 | 0.0005 | 0.0019 | 0.003 |
Nuclear Periphery | 0.0008 | 0.0008 | 0.0005 | 0.0008 | 0.0006 | 0.0004 | 0.007 | 0.0002 | 0.0005 | 0.0011 | 0.0078 | 0.0007 | 0.0091 | 0.0002 | 0.0021 | 0.0008 | 0.0023 | 0.0046 |
Nucleolus | 0.0006 | 0 | 0 | 0 | 0 | 0 | 0.0006 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0.0003 | 0 | 0.0005 | 0 | 0.0002 | 0.0006 |
Nucleus | 0.0012 | 0.0009 | 0.001 | 0.0015 | 0.0012 | 0.0008 | 0.0047 | 0.0006 | 0.0011 | 0.0021 | 0.0028 | 0.0014 | 0.0037 | 0.0006 | 0.0021 | 0.0013 | 0.0011 | 0.0024 |
Peroxisomes | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0066 | 0 | 0.0013 | 0.0051 | 0.0002 | 0.0003 | 0.006 | 0.0006 | 0.0081 | 0.0024 | 0.0269 | 0.0003 |
Punctate Nuclear | 0.0027 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0043 | 0 | 0.0001 | 0.0004 | 0.0004 | 0.0002 | 0.0017 | 0 | 0.0013 | 0.0001 | 0.008 | 0.0023 |
Vacuole | 0.0013 | 0.0012 | 0.0011 | 0.005 | 0.0008 | 0.0008 | 0.0059 | 0.0004 | 0.001 | 0.0023 | 0.0129 | 0.0012 | 0.0028 | 0.0005 | 0.0029 | 0.0027 | 0.0008 | 0.0008 |
Vacuole Periphery | 0.0002 | 0.0002 | 0.0003 | 0.0014 | 0.0002 | 0.0003 | 0.0027 | 0.0001 | 0.0003 | 0.0006 | 0.007 | 0.0004 | 0.0014 | 0.0001 | 0.001 | 0.0008 | 0.0004 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 26.611 | 33.9837 | 34.0434 | 36.4164 | 19.7156 | 18.0474 | 33.9313 | 35.6566 | 27.9943 | 34.5608 |
Translational Efficiency | 2.0903 | 1.7324 | 1.5477 | 1.557 | 2.1996 | 2.4422 | 1.582 | 1.4535 | 1.6586 | 1.5777 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✔ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2102 | 336 | 258 | 1206 | 1838 | 485 | 335 | 731 | 3940 | 821 | 593 | 1937 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1374.03 | 1418.36 | 1658.04 | 1461.69 | 1298.40 | 1353.51 | 1721.39 | 1574.35 | 1338.75 | 1380.05 | 1693.83 | 1504.21 |
Standard Deviation | 203.47 | 227.91 | 202.80 | 258.25 | 199.72 | 198.32 | 229.82 | 315.62 | 205.22 | 213.33 | 220.72 | 286.53 |
Intensity Change Log 2 | — | 0.045810 | 0.271065 | 0.089224 | — | 0.059971 | 0.406839 | 0.278021 | — | 0.052707 | 0.338628 | 0.184039 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000118 | 0.000267 | 0.000756 | 0.000605 | 0.000130 | 0.000142 | 0.001110 | 0.000646 | 0.000124 | 0.000193 | 0.000956 | 0.000621 |
Bud Neck | 0.000162 | 0.000512 | 0.004089 | 0.001203 | 0.000201 | 0.000248 | 0.003094 | 0.001713 | 0.000180 | 0.000356 | 0.003527 | 0.001396 |
Bud Site | 0.007153 | 0.014700 | 0.023363 | 0.024849 | 0.008577 | 0.006934 | 0.024209 | 0.023880 | 0.007817 | 0.010112 | 0.023841 | 0.024484 |
Cell Periphery | 0.000172 | 0.000425 | 0.000725 | 0.002015 | 0.000174 | 0.000095 | 0.000733 | 0.001939 | 0.000173 | 0.000230 | 0.000730 | 0.001987 |
Cytoplasm | 0.920782* | 0.880248* | 0.630617* | 0.864774* | 0.887612* | 0.908603* | 0.825600* | 0.844912* | 0.905308* | 0.896998* | 0.740768* | 0.857278* |
Cytoplasmic Foci | 0.044106 | 0.059206 | 0.075600 | 0.016569 | 0.060521 | 0.052498 | 0.056658 | 0.022406 | 0.051763 | 0.055243 | 0.064899 | 0.018772 |
Eisosomes | 0.000002 | 0.000003 | 0.000010 | 0.000004 | 0.000002 | 0.000002 | 0.000008 | 0.000006 | 0.000002 | 0.000003 | 0.000008 | 0.000005 |
Endoplasmic Reticulum | 0.001753 | 0.002605 | 0.003231 | 0.000749 | 0.002823 | 0.001144 | 0.000745 | 0.000851 | 0.002252 | 0.001742 | 0.001827 | 0.000787 |
Endosome | 0.001344 | 0.003655 | 0.018666 | 0.002957 | 0.002723 | 0.002450 | 0.005914 | 0.003867 | 0.001987 | 0.002943 | 0.011462 | 0.003301 |
Golgi | 0.007710 | 0.006555 | 0.004799 | 0.005224 | 0.007490 | 0.004262 | 0.005006 | 0.004226 | 0.007608 | 0.005201 | 0.004916 | 0.004847 |
Lipid Particles | 0.000842 | 0.003296 | 0.009910 | 0.006546 | 0.000620 | 0.000861 | 0.006815 | 0.008538 | 0.000738 | 0.001858 | 0.008162 | 0.007298 |
Mitochondria | 0.000872 | 0.000258 | 0.000015 | 0.000049 | 0.000291 | 0.000077 | 0.000011 | 0.000067 | 0.000601 | 0.000151 | 0.000013 | 0.000056 |
Mitotic Spindle | 0.000640 | 0.000790 | 0.002994 | 0.001932 | 0.001300 | 0.000510 | 0.003028 | 0.001271 | 0.000948 | 0.000625 | 0.003013 | 0.001683 |
None | 0.000798 | 0.000410 | 0.000113 | 0.000302 | 0.000632 | 0.000408 | 0.000403 | 0.000484 | 0.000721 | 0.000409 | 0.000277 | 0.000371 |
Nuclear Periphery | 0.000016 | 0.000310 | 0.000134 | 0.000023 | 0.000016 | 0.000012 | 0.000065 | 0.000052 | 0.000016 | 0.000134 | 0.000095 | 0.000034 |
Nuclear Periphery Foci | 0.000673 | 0.003808 | 0.007981 | 0.001901 | 0.000693 | 0.000848 | 0.006691 | 0.003235 | 0.000682 | 0.002060 | 0.007252 | 0.002404 |
Nucleolus | 0.000050 | 0.000171 | 0.000082 | 0.000060 | 0.000030 | 0.000028 | 0.000047 | 0.000094 | 0.000040 | 0.000087 | 0.000062 | 0.000073 |
Nucleus | 0.002558 | 0.002513 | 0.006489 | 0.004361 | 0.003089 | 0.003820 | 0.009099 | 0.005608 | 0.002806 | 0.003285 | 0.007964 | 0.004832 |
Peroxisomes | 0.000118 | 0.000419 | 0.000733 | 0.000316 | 0.000113 | 0.000144 | 0.000413 | 0.000534 | 0.000116 | 0.000256 | 0.000552 | 0.000398 |
Vacuole | 0.005514 | 0.016030 | 0.207257* | 0.060719 | 0.015243 | 0.014959 | 0.048710 | 0.073707 | 0.010053 | 0.015398 | 0.117690 | 0.065621 |
Vacuole Periphery | 0.004617 | 0.003816 | 0.002436 | 0.004841 | 0.007721 | 0.001955 | 0.001640 | 0.001963 | 0.006065 | 0.002717 | 0.001987 | 0.003755 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.65 | -7.22 | -7.85 | -4.75 | 1.31 | -0.58 | -6.57 | -6.22 | -5.96 | 2.74 | -3.27 | -8.90 | -10.00 | -8.13 | 3.18 |
Bud Neck | -4.55 | -11.83 | -11.79 | -6.34 | 8.62 | -1.34 | -9.65 | -9.06 | -8.70 | 4.06 | -4.64 | -14.99 | -14.43 | -11.69 | 9.15 |
Bud Site | -4.46 | -7.96 | -9.46 | -4.26 | -0.48 | 2.03 | -6.32 | -6.23 | -6.81 | 0.44 | -2.68 | -9.64 | -11.26 | -8.83 | 0.00 |
Cell Periphery | -1.55 | -5.37 | -7.70 | -5.40 | -4.93 | 4.18 | -7.22 | -5.87 | -6.16 | -3.77 | -0.83 | -8.87 | -9.69 | -8.78 | -6.28 |
Cytoplasm | 4.04 | 17.11 | 10.06 | 2.02 | -13.13 | -2.75 | 6.86 | 6.34 | 7.54 | -2.01 | 1.40 | 16.66 | 11.14 | 6.83 | -10.84 |
Cytoplasmic Foci | -3.00 | -6.63 | 12.77 | 8.77 | 12.92 | 1.74 | 0.50 | 12.95 | 6.84 | 7.81 | -1.05 | -4.18 | 18.72 | 11.38 | 14.61 |
Eisosomes | -1.76 | -9.72 | -6.31 | -1.38 | 6.91 | 0.86 | -6.26 | -5.09 | -5.67 | 1.66 | -0.97 | -10.85 | -7.61 | -5.55 | 5.92 |
Endoplasmic Reticulum | -0.76 | -0.60 | 3.06 | 1.74 | 1.23 | 2.85 | 4.05 | 2.98 | 0.67 | -0.18 | 0.92 | 0.83 | 4.35 | 1.97 | 1.14 |
Endosome | -3.31 | -9.23 | -4.15 | 0.86 | 8.15 | 0.33 | -4.32 | -1.64 | -1.33 | 2.38 | -1.76 | -9.99 | -3.72 | -0.50 | 8.16 |
Golgi | 1.19 | 5.22 | 4.31 | 1.43 | -0.90 | 5.40 | 2.85 | 3.60 | 0.37 | 0.50 | 4.56 | 4.89 | 5.55 | 0.91 | -0.18 |
Lipid Particles | -1.71 | -9.85 | -7.29 | -2.09 | 3.11 | -0.70 | -8.87 | -8.24 | -7.57 | -1.31 | -1.79 | -13.13 | -10.82 | -6.44 | 1.39 |
Mitochondria | 1.50 | 2.45 | 2.36 | 1.01 | -5.08 | 1.54 | 2.04 | 1.64 | 0.44 | -1.50 | 2.09 | 2.98 | 2.77 | 1.13 | -3.12 |
Mitotic Spindle | -0.64 | -4.27 | -2.01 | -1.83 | 1.67 | 1.84 | -2.30 | 0.16 | -1.37 | 2.32 | 1.37 | -4.19 | -1.57 | -2.34 | 2.52 |
None | 1.68 | 3.09 | 2.24 | 1.34 | -3.41 | 2.33 | 2.90 | 1.14 | -0.34 | -0.56 | 2.38 | 3.64 | 2.70 | 0.69 | -1.37 |
Nuclear Periphery | -1.31 | -8.95 | -1.74 | 1.27 | 8.79 | 2.20 | -8.12 | -1.72 | -1.93 | 1.26 | -1.28 | -11.59 | -2.28 | 1.06 | 6.88 |
Nuclear Periphery Foci | -2.01 | -6.68 | -8.38 | 1.13 | 5.53 | -0.61 | -9.96 | -6.10 | -5.09 | 4.66 | -2.09 | -11.19 | -9.44 | -0.78 | 7.90 |
Nucleolus | -0.88 | -1.41 | -0.48 | 0.81 | 2.15 | 0.60 | -4.67 | -7.29 | -7.42 | -4.74 | -0.81 | -1.82 | -2.43 | 0.23 | -1.05 |
Nucleus | 0.26 | -7.41 | -6.40 | -6.25 | 3.76 | -2.15 | -7.23 | -4.52 | -3.51 | 2.91 | -2.05 | -9.81 | -7.24 | -5.65 | 4.99 |
Peroxisomes | -2.62 | -4.79 | -7.23 | 0.88 | 3.10 | -1.68 | -6.07 | -5.68 | -5.16 | -1.23 | -2.90 | -7.08 | -8.64 | -2.43 | 2.13 |
Vacuole | -3.06 | -13.97 | -16.76 | -9.99 | 9.67 | 0.10 | -8.47 | -13.00 | -12.01 | -2.82 | -2.44 | -15.09 | -20.70 | -15.78 | 6.74 |
Vacuole Periphery | 0.44 | 3.17 | -0.03 | -0.46 | -3.40 | 5.05 | 5.67 | 6.04 | 0.24 | -0.53 | 3.42 | 6.30 | 3.39 | -0.95 | -3.22 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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