Standard name
Human Ortholog
Description Poly(A+) RNA-binding protein; key surveillance factor for the selective export of spliced mRNAs from the nucleus to the cytoplasm; preference for intron-containing genes; similar to Npl3p; HRB1 has a paralog, GBP2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0.05 0 0 0 0 0 0.1 0.1 0.17 0.12 0.11 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.07 0.05 0 0.07 0.08 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.95 0.97 0.96 0.94 0.89 0.85 0.81 0.81 0.8 0.79 0.78 0.77 0.88 0.86 0.78 0.81 0.81 0.83 0.92 0.93 0.94 0.84 0.75 0.61
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.06 0 0.05 0.06 0.17 0.28 0.28 0.32 0.4 0.41 0.37 0.38 0 0 0 0 0.05 0.05 0 0 0 0 0.07 0.13
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.07 0.14 0.22
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 2 0 0 0 1 1 1 0 0 0 0 0 0 0 1 1 4 8 5
Bud Neck 0 0 0 0 0 1 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 1 2 3 6 6 11 8 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0 0 0
Cytoplasm 4 2 3 4 7 4 17 8 4 4 5 1 39 47 83 19 28 13 0 0 0 5 1 1
Endoplasmic Reticulum 0 0 0 0 2 1 0 0 0 0 0 0 0 0 1 4 2 2 0 0 0 0 2 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 0 1 0 4 1 1 2 3 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 1 0 1 0 0
Mitochondria 3 3 0 0 4 7 23 17 11 25 19 33 0 0 0 2 3 5 0 0 0 0 4 4
Nucleus 222 168 211 122 144 181 261 267 238 289 182 271 349 420 386 129 210 110 219 165 216 280 465 335
Nuclear Periphery 0 0 0 0 1 3 3 1 3 0 0 5 0 1 2 0 0 0 0 0 0 0 1 3
Nucleolus 14 5 11 8 27 59 91 106 120 149 87 135 7 17 20 7 12 6 3 2 6 8 40 71
Peroxisomes 1 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 4 6 13 1 0 0 0 0 0 1 2 4
Vac/Vac Membrane 0 2 1 2 0 1 2 2 3 2 2 0 4 5 12 3 13 4 4 3 3 24 90 121
Unique Cell Count 233 174 219 130 161 214 323 329 297 367 234 352 396 486 498 160 259 133 239 179 230 334 623 553
Labelled Cell Count 244 180 226 136 186 261 400 407 385 481 304 449 404 499 524 165 270 140 239 179 230 334 623 553


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.3 16.3 15.5 12.2 11.0 9.2 8.9 8.9 8.4 8.2 7.6 7.6 16.9 16.1 15.6 18.4 17.8 18.0 14.6 16.2 17.3
Std Deviation (1e-4) 4.0 4.8 4.7 2.8 2.7 2.6 3.2 3.4 2.4 2.8 2.4 2.2 6.7 6.5 8.1 7.6 6.6 7.3 4.5 5.3 5.5
Intensity Change (Log2) – – – -0.35 -0.49 -0.75 -0.8 -0.8 -0.89 -0.91 -1.02 -1.02 0.13 0.05 0.01 0.25 0.2 0.22 -0.08 0.07 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 2.4 0 0 0 0 0 4.0 4.0 5.8 4.3 4.2 3.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 4.0 3.4 0 3.9 4.3 4.7 0 0 0 0 0 0
Nucleus -1.1 -2.7 -4.2 -5.3 -5.2 -5.4 -5.8 -5.8 -6.2 -3.4 -4.0 -6.2 -5.0 -5.1 -4.4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.5 3.8 6.4 6.8 7.6 9.1 9.4 8.3 8.9 -2.3 -1.0 -0.6 -0.3 -0.2 -0.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 2.9 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.9891 11.1688 10.783 9.848 10.3752 10.4236 10.8418 11.9962 10.8259 10.8156 9.4852 10.5 11.6862 12.493 11.4138 10.0635 10.7242 11.0251
Actin 0 0 0 0 0 0 0.0169 0.0033 0.0115 0.0035 0.0046 0.0032 0.0088 0 0.0179 0 0.005 0.0003
Bud 0 0 0 0 0 0 0.0014 0 0.0003 0.0001 0.0013 0.0001 0.0003 0 0.0001 0 0.0008 0
Bud Neck 0.0001 0 0 0 0.0001 0.0002 0.001 0 0.0002 0.0001 0.0003 0.0007 0.0004 0 0.0001 0 0.0006 0.0002
Bud Periphery 0 0 0 0 0 0 0.0028 0 0.0003 0.0001 0.0015 0.0001 0.0006 0 0.0001 0 0.0013 0.0001
Bud Site 0 0 0 0 0 0 0.0042 0.0001 0.0012 0.0002 0.0099 0.0001 0.0004 0 0.0002 0 0.0019 0.0001
Cell Periphery 0 0 0 0 0 0 0.0007 0 0.0001 0 0.0005 0.0001 0.0004 0 0.0001 0 0.0006 0.0001
Cytoplasm 0.0009 0 0.0001 0 0 0 0.008 0.0003 0.0001 0.0001 0.006 0.0001 0.0025 0 0.001 0 0.0046 0.0011
Cytoplasmic Foci 0.0001 0 0 0 0 0 0.0176 0.0007 0.0012 0.0049 0.0081 0.0007 0.0032 0 0.0019 0 0.0089 0.0056
Eisosomes 0 0 0 0 0 0 0.0004 0 0 0 0.0001 0 0.0002 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0001 0 0 0 0 0 0.0016 0.0001 0.0001 0 0.0018 0.0005 0.0036 0 0.0005 0 0.0021 0.0001
Endosome 0.0005 0 0.0001 0 0 0 0.01 0.0009 0.0018 0.004 0.0235 0.0032 0.0078 0 0.0021 0 0.0077 0.0008
Golgi 0 0 0 0 0 0 0.0063 0.0009 0.0046 0.009 0.006 0.0025 0.0023 0 0.0021 0 0.0014 0.0003
Lipid Particles 0.0001 0 0 0 0 0 0.0515 0.0006 0.0021 0.008 0.0031 0.0031 0.0039 0 0.0018 0 0.008 0.0026
Mitochondria 0.0005 0.0001 0.0006 0.0001 0.0006 0.0005 0.0098 0.0004 0.0044 0.0042 0.0269 0.003 0.0051 0.0001 0.0008 0 0.0058 0.0002
None 0.004 0 0.0001 0 0 0 0.0025 0 0 0 0.0016 0.0002 0.0014 0 0.0007 0 0.0029 0.0004
Nuclear Periphery 0.005 0.0003 0.0024 0.0002 0.0011 0.0001 0.0073 0.0011 0.0004 0.0009 0.0043 0.0028 0.0182 0.0009 0.0032 0.0002 0.0254 0.0008
Nucleolus 0.0369 0.0091 0.0111 0.026 0.1276 0.06 0.0265 0.0094 0.0079 0.0067 0.0887 0.0674 0.0605 0.0094 0.0074 0.0085 0.1177 0.0546
Nucleus 0.9472 0.9904 0.9845 0.9736 0.8688 0.9387 0.7948 0.9813 0.9599 0.9422 0.7976 0.8994 0.8648 0.9893 0.9481 0.9912 0.7849 0.9244
Peroxisomes 0 0 0 0 0 0 0.0233 0.0006 0.0009 0.0155 0.0017 0.0003 0.0008 0 0.0086 0 0.001 0.0002
Punctate Nuclear 0.004 0 0.0001 0 0.0014 0 0.0065 0.0001 0.0001 0.0002 0.0016 0.0118 0.0108 0 0.0026 0 0.0119 0.0077
Vacuole 0.0004 0 0.0004 0 0.0001 0.0002 0.0042 0.0001 0.0015 0.0001 0.0039 0.0003 0.0019 0.0001 0.0002 0 0.0042 0.0002
Vacuole Periphery 0.0002 0 0.0006 0 0.0002 0.0001 0.0027 0.0002 0.0015 0.0001 0.0068 0.0004 0.0022 0.0001 0.0002 0 0.0031 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.6339 29.6274 24.6659 29.7757 28.2978 22.6214 29.7736 28.1086 32.1764 29.7395
Translational Efficiency 2.1118 1.6529 1.8448 1.5855 1.6338 1.9901 1.7165 1.686 1.5459 1.8199

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1605 1288 1908 832 1765 1990 702 1436 3370 3278 2610 2268

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1281.33 1606.54 1468.37 1433.91 1326.87 1425.85 1490.09 1312.09 1305.18 1496.85 1474.21 1356.78
Standard Deviation 275.27 332.75 282.03 258.35 256.30 282.34 228.65 308.61 266.48 315.73 268.89 297.04
Intensity Change Log 2 — 0.326315 0.196573 0.162312 — 0.103795 0.167372 -0.016160 — 0.217401 0.181792 0.074277

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000410 0.000429 0.000389 0.000799 0.000435 0.000443 0.000582 0.000914 0.000423 0.000437 0.000441 0.000872
Bud Neck 0.002524 0.005008 0.008356 0.016471 0.003669 0.004913 0.009852 0.019891 0.003123 0.004950 0.008758 0.018636
Bud Site 0.000211 0.000579 0.000425 0.001780 0.000389 0.000565 0.000625 0.005629 0.000305 0.000570 0.000479 0.004217
Cell Periphery 0.000093 0.000184 0.000045 0.000089 0.000134 0.000214 0.000038 0.000318 0.000115 0.000202 0.000043 0.000234
Cytoplasm 0.000364 0.000291 0.000038 0.000400 0.000078 0.000231 0.000118 0.003643 0.000214 0.000255 0.000059 0.002453
Cytoplasmic Foci 0.000032 0.000270 0.000012 0.001743 0.000034 0.000075 0.000032 0.001646 0.000033 0.000152 0.000017 0.001682
Eisosomes 0.000146 0.000179 0.000096 0.000044 0.000250 0.000175 0.000088 0.000042 0.000201 0.000176 0.000094 0.000043
Endoplasmic Reticulum 0.001404 0.000795 0.000616 0.001726 0.001439 0.001282 0.001000 0.001854 0.001422 0.001091 0.000719 0.001807
Endosome 0.000029 0.000110 0.000011 0.003364 0.000032 0.000092 0.000016 0.001800 0.000030 0.000099 0.000013 0.002374
Golgi 0.000033 0.000054 0.000015 0.001924 0.000050 0.000055 0.000027 0.001242 0.000042 0.000054 0.000018 0.001492
Lipid Particles 0.000018 0.000063 0.000003 0.000162 0.000011 0.000023 0.000009 0.000171 0.000015 0.000039 0.000004 0.000168
Mitochondria 0.001513 0.000769 0.000876 0.006586 0.001164 0.000846 0.001136 0.002967 0.001330 0.000816 0.000946 0.004295
Mitotic Spindle 0.000174 0.001645 0.000026 0.003234 0.000026 0.001428 0.001271 0.009739 0.000096 0.001513 0.000361 0.007353
None 0.000857 0.000941 0.000324 0.000645 0.000539 0.000556 0.000605 0.008464 0.000691 0.000707 0.000400 0.005596
Nuclear Periphery 0.000028 0.000052 0.000079 0.000377 0.000030 0.000153 0.000059 0.000714 0.000029 0.000113 0.000074 0.000591
Nuclear Periphery Foci 0.000038 0.000470 0.000019 0.000181 0.000048 0.000120 0.000006 0.000854 0.000043 0.000257 0.000015 0.000607
Nucleolus 0.015312 0.016294 0.008099 0.009251 0.020641 0.015965 0.009364 0.009754 0.018103 0.016094 0.008439 0.009569
Nucleus 0.975237* 0.969983* 0.979844* 0.945942* 0.969427* 0.970204* 0.973995* 0.924576* 0.972194* 0.970117* 0.978271* 0.932414*
Peroxisomes 0.000551 0.000791 0.000403 0.000428 0.000706 0.000551 0.000789 0.000416 0.000632 0.000645 0.000507 0.000420
Vacuole 0.000806 0.000748 0.000195 0.002618 0.000671 0.001068 0.000327 0.002743 0.000735 0.000942 0.000230 0.002697
Vacuole Periphery 0.000220 0.000348 0.000128 0.002236 0.000229 0.001042 0.000062 0.002623 0.000224 0.000769 0.000110 0.002481

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.75 0.54 -4.43 -4.27 -4.64 -0.32 -6.29 -2.64 -2.60 -1.68 -0.80 -1.92 -3.88 -3.76 -3.63
Bud Neck -4.05 -16.09 -9.78 -7.73 -6.07 -3.05 -12.09 -11.41 -10.53 -6.69 -5.38 -18.52 -14.97 -13.01 -9.64
Bud Site -2.53 -5.26 -5.13 -3.65 -4.52 -1.36 -3.36 -4.84 -4.64 -4.55 -2.81 -3.97 -5.78 -5.30 -5.49
Cell Periphery -1.07 2.27 0.28 1.17 -2.18 -0.87 2.24 -2.09 -0.93 -3.65 -1.38 2.91 -2.15 -0.36 -3.87
Cytoplasm 0.47 1.61 0.14 -0.58 -3.88 -0.93 -0.16 -4.05 -3.82 -4.04 0.02 1.66 -3.81 -3.86 -4.37
Cytoplasmic Foci -1.56 1.84 -1.93 -1.52 -1.96 -0.86 0.45 -2.62 -2.53 -2.64 -1.79 2.02 -3.26 -2.91 -3.30
Eisosomes -1.50 3.22 7.33 7.66 6.17 2.72 6.36 8.27 11.06 12.10 1.37 6.76 10.68 13.32 8.59
Endoplasmic Reticulum 4.75 6.77 -0.66 -2.17 -2.62 1.41 3.87 -2.92 -4.12 -5.99 3.89 9.33 -2.14 -4.10 -6.30
Endosome -1.38 2.19 -2.90 -2.81 -2.92 -1.60 1.40 -4.22 -4.01 -4.26 -2.11 2.64 -4.71 -4.52 -4.76
Golgi -0.80 1.18 -2.64 -2.60 -2.67 -0.07 0.74 -2.70 -2.70 -2.78 -0.49 1.38 -3.78 -3.75 -3.87
Lipid Particles -0.99 1.75 -1.66 -0.96 -1.92 -0.90 0.41 -2.57 -2.33 -2.64 -1.22 2.01 -3.01 -2.31 -3.31
Mitochondria 1.12 0.94 -2.38 -2.94 -2.87 0.58 -0.05 -2.61 -4.76 -4.03 1.21 0.84 -3.34 -4.56 -4.35
Mitotic Spindle -1.52 1.28 -2.27 -1.09 -2.36 -2.11 -1.02 -5.10 -4.17 -3.84 -2.54 -0.80 -5.56 -4.17 -5.24
None -0.29 2.46 0.71 0.97 -5.94 -0.11 -0.40 -4.94 -4.93 -4.88 -0.12 2.42 -4.79 -4.76 -5.16
Nuclear Periphery -2.04 -1.21 -5.47 -5.09 -4.07 -2.55 -4.03 -5.28 -4.07 -5.00 -2.84 -1.50 -6.70 -5.42 -5.78
Nuclear Periphery Foci -1.27 1.15 -1.12 0.99 -2.79 -1.13 1.53 -2.26 -2.01 -2.45 -1.52 1.60 -2.38 -1.17 -2.70
Nucleolus -0.71 5.49 2.09 2.50 -1.56 2.92 5.94 4.94 2.35 -1.41 1.65 9.20 4.93 3.40 -2.70
Nucleus 1.96 -2.24 5.71 4.51 6.60 -0.25 -1.20 9.14 9.06 9.42 1.26 -3.76 10.94 10.02 12.51
Peroxisomes -1.95 1.20 -0.16 1.66 -1.28 1.36 -1.15 2.96 1.08 3.51 -0.32 1.63 1.79 1.91 0.62
Vacuole 0.35 3.20 -2.38 -2.50 -3.20 -1.49 2.05 -3.63 -2.83 -4.44 -0.89 4.10 -4.19 -3.79 -5.53
Vacuole Periphery -1.39 2.10 -1.91 -1.75 -2.02 -2.15 3.01 -2.63 -1.34 -2.87 -2.36 2.94 -3.25 -2.10 -3.47
Standard name
Human Ortholog
Description Poly(A+) RNA-binding protein; key surveillance factor for the selective export of spliced mRNAs from the nucleus to the cytoplasm; preference for intron-containing genes; similar to Npl3p; HRB1 has a paralog, GBP2, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Hrb1

Hrb1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hrb1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available