Standard name
Human Ortholog
Description Subunit of the inner nuclear membrane Asi ubiquitin ligase complex; Asi complex targets both misfolded proteins of the inner nuclear membrane-associated degradation (INMAD) pathway and regulators of sterol biosynthesis for ubiquitin-mediated degradation; acts with Asi1p and Asi2p to ensure the fidelity of SPS-sensor signaling by targeting latent unprocessed forms of Stp1p and Stp2p, maintaining the repressed state of gene expression in the absence of inducing amino acids
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.06 0 0.1 0.07 0.1 0.07 0.08 0.13 0.08 0.09 0.13 0.07 0.13 0.17 0.21 0.1 0.12 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.27 0.05 0 0.14 0 0.1 0.2 0.13 0.37 0.24 0.49 0.38 0 0 0 0.1 0 0.08 0 0 0.05 0 0.06 0.09
Nucleus 0.09 0.09 0.29 0.33 0.3 0.25 0.35 0.39 0.3 0.36 0.26 0.33 0.2 0.23 0.28 0.14 0.08 0.24 0 0 0 0.11 0.13 0.13
Nuclear Periphery 0.53 0.59 0.24 0.35 0.25 0.28 0.3 0.19 0.19 0.16 0.16 0.15 0.36 0.38 0.29 0.4 0.54 0.24 0.82 0.84 0.71 0.6 0.47 0.35
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0.05 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.46 0.59 0.62 0.31 0.44 0.5 0.38 0.33 0.34 0.37 0.24 0.31 0.57 0.38 0.4 0.4 0.58 0.73 0.05 0 0.08 0.11 0.19 0.27
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0
Bud 0 0 0 0 0 0 2 4 6 3 1 5 0 0 0 0 0 0 0 1 0 1 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 1 0 0 0 0 0 0
Cytoplasm 16 5 4 7 19 13 19 39 19 23 26 16 19 34 47 6 3 2 2 0 0 4 3 5
Endoplasmic Reticulum 5 4 0 0 1 2 0 0 0 0 4 0 1 4 1 4 1 1 2 0 2 1 4 2
Endosome 1 0 0 2 3 1 4 4 2 6 3 4 2 2 2 1 1 0 3 3 0 1 5 4
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 1 2 0 1 0 0 0
Mitochondria 69 7 0 15 5 19 45 38 93 58 95 83 1 5 2 6 1 3 11 3 4 4 12 19
Nucleus 24 12 12 34 57 47 81 116 75 89 50 72 30 46 64 9 2 9 3 3 3 12 27 28
Nuclear Periphery 137 79 10 36 48 53 69 56 47 39 31 32 54 78 65 25 13 9 235 127 61 68 95 75
Nucleolus 2 0 0 0 0 1 0 1 1 1 1 2 0 1 0 5 1 2 1 1 0 1 7 4
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 1 0 0 0 0 0
SpindlePole 0 0 0 0 1 1 0 2 1 2 1 4 1 2 3 0 0 0 3 1 1 2 3 5
Vac/Vac Membrane 119 79 26 32 84 93 88 99 86 91 46 69 84 78 91 25 14 27 12 6 6 12 39 58
Unique Cell Count 257 133 42 104 192 186 230 299 251 244 194 220 148 203 227 63 24 37 287 152 87 114 204 213
Labelled Cell Count 373 186 52 126 219 230 309 359 330 312 258 288 192 254 276 81 36 55 287 152 87 114 204 213


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 5.6 6.2 6.0 7.2 5.8 5.6 6.1 5.0 5.1 4.8 4.7 6.5 6.2 6.3 7.6 8.9 7.7 5.9 6.4 6.7
Std Deviation (1e-4) 0.7 0.9 0.9 1.9 2.3 2.0 1.8 2.2 1.6 1.4 2.0 1.5 1.2 1.4 1.6 2.0 1.6 1.6 1.2 1.6 1.2
Intensity Change (Log2) – – – -0.04 0.21 -0.09 -0.15 -0.02 -0.33 -0.27 -0.38 -0.39 0.06 -0.01 0.03 0.29 0.52 0.3 -0.07 0.05 0.11

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP7000510WT3HU80HU120HU1600510WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30510WT1AF100AF140AF1800510
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.6 0 0 0.7 0 0.6 1.2 1.7 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.1 0 4.8 3.5 0 0 0 0 0 0 0 0
Nucleus 0.5 0.1 -0.4 0.8 1.3 0.2 1.0 -0.4 0.5 -1.1 -0.8 0 -1.8 -1.9 -0.4
Nuclear Periphery 1.3 0.2 0.6 0.8 -0.8 -0.8 -1.2 0 0 1.5 1.8 0.6 1.7 2.5 0.1
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -3.5 -2.1 -1.4 -2.9 -3.6 -3.4 -3.0 -4.9 -3.8 -0.6 -2.8 -2.6 -2.2 -0.3 1.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.0165 0.9696 0.7181 0.3622 0.4742 0.5814 1.3891 1.5267 1.3889 1.1586 0.8981 1.342 1.2065 0.942 0.9717 0.8487 1.0206 0.7545
Actin 0.0013 0.0008 0.0084 0.0005 0.005 0.0001 0.0123 0.0002 0.0002 0.0017 0.0005 0.0004 0.0086 0.0001 0.0056 0.0001 0.02 0.0012
Bud 0.0001 0.0001 0.0003 0.0001 0.0001 0 0.0004 0 0 0.0001 0.0001 0 0.0001 0 0.0001 0 0.0015 0.0001
Bud Neck 0.0004 0.0032 0.0002 0.0001 0.0003 0 0.0012 0 0 0.0001 0.0001 0.0001 0.0009 0 0.0004 0 0.0003 0.0001
Bud Periphery 0.0001 0.0001 0.0005 0.0005 0.0002 0.0001 0.0007 0 0.0001 0.0002 0.0001 0.0002 0.0002 0 0.0002 0 0.005 0.0003
Bud Site 0.0004 0.0027 0.0017 0.0002 0.0002 0 0.0046 0 0 0.0002 0.0002 0 0.0008 0 0.0057 0 0.0013 0.0001
Cell Periphery 0.0001 0.0001 0.0001 0.0002 0 0 0.0003 0 0 0.0001 0 0 0.0001 0 0.0001 0 0.0002 0
Cytoplasm 0.0032 0.0031 0.0027 0.0021 0.0021 0.0007 0.008 0.0031 0.0007 0.0008 0.0007 0.001 0.0022 0.0107 0.0043 0.0001 0.0018 0.0035
Cytoplasmic Foci 0.0054 0.0005 0.0029 0.0013 0.0019 0.0002 0.0138 0.0001 0.005 0.0058 0.0058 0.0006 0.0162 0.0001 0.0016 0 0.0048 0.0028
Eisosomes 0.0001 0 0.0001 0 0.0004 0 0.0002 0 0 0 0 0 0.0001 0 0.0001 0 0.0003 0.0001
Endoplasmic Reticulum 0.0102 0.0116 0.0114 0.0111 0.0037 0.003 0.0105 0.0167 0.018 0.035 0.004 0.0048 0.0092 0.0179 0.0095 0.0084 0.005 0.0061
Endosome 0.0255 0.0017 0.0074 0.0198 0.0144 0.002 0.0179 0.0011 0.0079 0.0044 0.0229 0.0037 0.0174 0.0012 0.0059 0.0018 0.0346 0.0061
Golgi 0.0041 0.0002 0.0025 0.0025 0.0052 0.0004 0.0051 0 0.001 0.0024 0.0193 0.003 0.0056 0 0.0022 0.0001 0.0202 0.0011
Lipid Particles 0.0326 0.0006 0.0056 0.0007 0.0052 0.0034 0.0169 0.0002 0.0096 0.0116 0.0028 0.0076 0.012 0.0002 0.0058 0.0003 0.0311 0.0032
Mitochondria 0.0023 0.0009 0.0093 0.0149 0.0022 0.0009 0.0112 0.0006 0.0046 0.0615 0.0064 0.0131 0.0031 0.0006 0.006 0.002 0.0204 0.004
None 0.0086 0.0011 0.0082 0.0003 0.027 0.0007 0.0172 0.0135 0.0038 0.0007 0.0005 0.0053 0.0016 0.0009 0.0013 0.0002 0.0089 0.0026
Nuclear Periphery 0.8523 0.8766 0.8768 0.8944 0.7882 0.9399 0.8194 0.8857 0.8888 0.779 0.8595 0.9043 0.854 0.8644 0.8905 0.9382 0.7532 0.9135
Nucleolus 0.0009 0.001 0.0002 0.0001 0.0014 0.0003 0.0008 0.0004 0.0002 0.0001 0.0004 0.0006 0.0014 0.0016 0.0003 0.0001 0.0008 0.0004
Nucleus 0.0157 0.0528 0.0131 0.0114 0.1198 0.0297 0.0134 0.0312 0.0089 0.0153 0.0379 0.0318 0.0178 0.0675 0.0171 0.0082 0.0614 0.0327
Peroxisomes 0.0025 0.0001 0.0035 0.0001 0.0008 0.0001 0.0101 0 0.0037 0.0445 0.0011 0.0008 0.011 0 0.0013 0 0.0083 0.001
Punctate Nuclear 0.0043 0.0042 0.0015 0.0003 0.0042 0.0011 0.0026 0.0136 0.0005 0.0012 0.0209 0.0031 0.0065 0.0034 0.0009 0.0002 0.0029 0.0056
Vacuole 0.0032 0.0013 0.0021 0.0041 0.001 0.0019 0.0045 0.0011 0.0019 0.002 0.001 0.002 0.0018 0.0015 0.0017 0.0008 0.0024 0.0012
Vacuole Periphery 0.0268 0.0375 0.0418 0.0354 0.0168 0.0155 0.0291 0.0324 0.0449 0.0334 0.0157 0.0175 0.0295 0.0297 0.0394 0.0393 0.0157 0.0144

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.1253 9.7132 9.0095 14.9844 11.2702 7.0587 16.9412 14.9142 15.5702 19.4964
Translational Efficiency 0.6558 0.8066 0.8895 0.5556 0.6109 1.2652 0.5009 0.4988 0.6522 0.5515

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
817 1588 1610 235 1962 2066 1796 1727 2779 3654 3406 1962

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 620.77 664.04 898.40 861.02 749.51 755.91 895.47 865.40 711.66 715.98 896.85 864.88
Standard Deviation 68.11 113.66 126.21 118.31 87.93 102.09 111.13 119.62 101.30 116.54 118.51 119.47
Intensity Change Log 2 — 0.097211 0.533299 0.471988 — 0.012267 0.256697 0.207419 — 0.051369 0.388605 0.333313

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000041 0.000151 0.000221 0.000385 0.000026 0.000091 0.000111 0.000132 0.000030 0.000117 0.000163 0.000162
Bud Neck 0.001659 0.001405 0.001761 0.003606 0.000157 0.001447 0.001003 0.002377 0.000598 0.001429 0.001361 0.002524
Bud Site 0.000348 0.000508 0.000732 0.003382 0.000042 0.001789 0.000304 0.000931 0.000132 0.001232 0.000506 0.001225
Cell Periphery 0.000088 0.000133 0.000040 0.000100 0.000039 0.000081 0.000032 0.000033 0.000054 0.000104 0.000036 0.000041
Cytoplasm 0.139914 0.083794 0.023860 0.051889 0.031472 0.036641 0.026665 0.046931 0.063353 0.057133 0.025339 0.047525
Cytoplasmic Foci 0.026012 0.010921 0.000379 0.006198 0.004607 0.007221 0.000477 0.001690 0.010900 0.008829 0.000430 0.002230
Eisosomes 0.000030 0.000022 0.000007 0.000045 0.000008 0.000013 0.000005 0.000007 0.000015 0.000017 0.000006 0.000012
Endoplasmic Reticulum 0.065609 0.039159 0.022862 0.018972 0.042735 0.047523 0.020628 0.026188 0.049460 0.043888 0.021684 0.025324
Endosome 0.005283 0.005656 0.001616 0.006317 0.001665 0.005739 0.001424 0.003414 0.002728 0.005703 0.001515 0.003761
Golgi 0.000806 0.000520 0.000032 0.000425 0.000195 0.001848 0.000031 0.000166 0.000375 0.001271 0.000031 0.000197
Lipid Particles 0.088262 0.021950 0.000616 0.008080 0.020710 0.020333 0.000969 0.001625 0.040569 0.021036 0.000802 0.002398
Mitochondria 0.009871 0.011528 0.002510 0.002301 0.003204 0.013903 0.001611 0.002465 0.005164 0.012871 0.002036 0.002445
Mitotic Spindle 0.000616 0.001649 0.002944 0.014836 0.001325 0.004180 0.001127 0.011156 0.001117 0.003080 0.001986 0.011597
None 0.015697 0.025564 0.001703 0.007138 0.002566 0.005041 0.001762 0.002412 0.006426 0.013960 0.001734 0.002978
Nuclear Periphery 0.426756 0.459938 0.629522 0.299588 0.738054 0.648227 0.730730 0.658885 0.646535 0.566398 0.682890 0.615850
Nuclear Periphery Foci 0.076470 0.095583 0.045633 0.060264 0.083485 0.075029 0.059274 0.081633 0.081423 0.083962 0.052826 0.079073
Nucleolus 0.007017 0.028169 0.014664 0.014512 0.004846 0.008906 0.007737 0.009185 0.005484 0.017277 0.011012 0.009824
Nucleus 0.124571 0.195283 0.241658 0.477667 0.057717 0.099982 0.138895 0.129069 0.077371 0.141399 0.187471 0.170822
Peroxisomes 0.002258 0.000956 0.000174 0.000791 0.000383 0.000879 0.000115 0.000160 0.000934 0.000912 0.000143 0.000236
Vacuole 0.007028 0.012698 0.007390 0.021245 0.004871 0.014065 0.006090 0.019105 0.005505 0.013471 0.006705 0.019362
Vacuole Periphery 0.001662 0.004412 0.001676 0.002258 0.001894 0.007060 0.001008 0.002435 0.001826 0.005909 0.001324 0.002414

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.02 -9.30 -2.47 -1.76 -1.21 -6.11 -8.71 -8.92 -3.58 -1.93 -7.76 -12.77 -6.68 -2.66 -0.30
Bud Neck 0.26 -0.45 -2.14 -2.51 -2.13 -4.19 -4.75 -7.22 -3.91 -4.58 -2.69 -3.41 -6.30 -4.81 -4.56
Bud Site -0.90 -1.20 -1.96 -1.86 -1.63 -3.06 -2.42 -3.48 0.79 -2.47 -3.36 -2.16 -3.79 -0.71 -2.25
Cell Periphery -4.02 5.76 -1.03 1.34 -3.56 -5.39 0.81 0.85 6.16 -0.34 -7.79 3.14 2.09 9.30 -1.32
Cytoplasm 6.30 14.64 7.35 3.00 -3.83 -1.67 1.09 -5.67 -4.24 -6.72 1.46 11.28 2.22 1.02 -8.35
Cytoplasmic Foci 5.50 10.67 7.42 3.24 -5.22 -2.61 7.13 4.45 7.28 -4.26 1.88 12.47 9.73 9.87 -5.84
Eisosomes 3.17 10.29 -3.76 -5.22 -7.92 -4.26 2.63 -0.33 4.21 -3.22 -2.25 8.67 0.68 2.79 -7.03
Endoplasmic Reticulum 6.26 10.66 10.67 7.62 1.55 -1.67 10.22 7.45 8.97 -3.78 2.45 14.44 11.75 10.67 -3.02
Endosome -0.42 4.50 -0.87 -0.73 -2.68 -7.80 0.73 -5.90 3.39 -6.17 -6.92 4.16 -3.09 3.08 -6.42
Golgi 1.09 3.02 1.31 0.66 -1.59 -3.87 2.65 0.44 3.96 -2.06 -3.49 3.92 1.82 4.39 -2.47
Lipid Particles 13.17 18.16 16.14 7.52 -5.75 0.20 14.18 13.64 13.43 -3.57 9.40 21.85 20.85 18.38 -7.25
Mitochondria -0.99 4.99 4.88 8.66 0.57 -9.03 4.30 1.63 9.72 -3.13 -8.62 6.15 4.98 13.20 -1.74
Mitotic Spindle -2.63 -2.97 -2.97 -2.85 -2.64 -3.03 -0.58 -6.58 -4.79 -6.25 -3.29 -2.19 -7.27 -6.17 -6.40
None -3.20 5.85 2.43 5.35 -1.63 -3.59 1.89 0.23 3.49 -1.40 -6.18 6.09 3.86 9.97 -2.15
Nuclear Periphery -2.03 -12.97 6.32 7.94 14.97 9.02 1.67 10.14 1.56 8.02 9.18 -3.23 5.68 -2.10 8.58
Nuclear Periphery Foci -3.14 6.21 1.91 4.06 -1.65 2.09 5.98 0.76 -1.19 -4.86 -0.69 9.35 0.97 1.61 -6.98
Nucleolus -9.37 -4.33 -2.41 4.19 0.01 -3.88 -2.94 -4.11 -0.61 -1.51 -10.58 -6.15 -4.23 5.21 0.62
Nucleus -7.30 -11.49 -13.31 -10.49 -8.43 -7.51 -12.97 -13.05 -5.83 0.13 -12.59 -19.67 -14.88 -5.23 1.43
Peroxisomes 1.51 2.41 1.79 1.12 -2.05 -3.74 3.70 2.97 6.77 -1.85 -0.01 2.99 2.90 8.30 -0.56
Vacuole -4.09 -0.87 -4.81 -4.14 -4.67 -7.41 -3.11 -9.70 -4.57 -7.57 -8.82 -3.35 -10.58 -6.38 -9.04
Vacuole Periphery -5.16 -0.18 -0.31 1.70 -0.27 -7.33 3.43 -1.19 6.07 -3.47 -8.73 2.33 -1.37 6.23 -2.63
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Subunit of the inner nuclear membrane Asi ubiquitin ligase complex; Asi complex targets both misfolded proteins of the inner nuclear membrane-associated degradation (INMAD) pathway and regulators of sterol biosynthesis for ubiquitin-mediated degradation; acts with Asi1p and Asi2p to ensure the fidelity of SPS-sensor signaling by targeting latent unprocessed forms of Stp1p and Stp2p, maintaining the repressed state of gene expression in the absence of inducing amino acids
Localization
Cell Percentages nuclear periphery (66%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Asi3

Asi3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Asi3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available