Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of the inner nuclear membrane Asi ubiquitin ligase complex; Asi complex targets both misfolded proteins of the inner nuclear membrane-associated degradation (INMAD) pathway and regulators of sterol biosynthesis for ubiquitin-mediated degradation; acts with Asi1p and Asi2p to ensure the fidelity of SPS-sensor signaling by targeting latent unprocessed forms of Stp1p and Stp2p, maintaining the repressed state of gene expression in the absence of inducing amino acids |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 6 | 3 | 1 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 16 | 5 | 4 | 7 | 19 | 13 | 19 | 39 | 19 | 23 | 26 | 16 | 19 | 34 | 47 | 6 | 3 | 2 | 2 | 0 | 0 | 4 | 3 | 5 |
Endoplasmic Reticulum | 5 | 4 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 4 | 0 | 1 | 4 | 1 | 4 | 1 | 1 | 2 | 0 | 2 | 1 | 4 | 2 |
Endosome | 1 | 0 | 0 | 2 | 3 | 1 | 4 | 4 | 2 | 6 | 3 | 4 | 2 | 2 | 2 | 1 | 1 | 0 | 3 | 3 | 0 | 1 | 5 | 4 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 0 | 0 | 0 |
Mitochondria | 69 | 7 | 0 | 15 | 5 | 19 | 45 | 38 | 93 | 58 | 95 | 83 | 1 | 5 | 2 | 6 | 1 | 3 | 11 | 3 | 4 | 4 | 12 | 19 |
Nucleus | 24 | 12 | 12 | 34 | 57 | 47 | 81 | 116 | 75 | 89 | 50 | 72 | 30 | 46 | 64 | 9 | 2 | 9 | 3 | 3 | 3 | 12 | 27 | 28 |
Nuclear Periphery | 137 | 79 | 10 | 36 | 48 | 53 | 69 | 56 | 47 | 39 | 31 | 32 | 54 | 78 | 65 | 25 | 13 | 9 | 235 | 127 | 61 | 68 | 95 | 75 |
Nucleolus | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 1 | 2 | 0 | 1 | 0 | 5 | 1 | 2 | 1 | 1 | 0 | 1 | 7 | 4 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 1 | 2 | 1 | 4 | 1 | 2 | 3 | 0 | 0 | 0 | 3 | 1 | 1 | 2 | 3 | 5 |
Vac/Vac Membrane | 119 | 79 | 26 | 32 | 84 | 93 | 88 | 99 | 86 | 91 | 46 | 69 | 84 | 78 | 91 | 25 | 14 | 27 | 12 | 6 | 6 | 12 | 39 | 58 |
Unique Cell Count | 257 | 133 | 42 | 104 | 192 | 186 | 230 | 299 | 251 | 244 | 194 | 220 | 148 | 203 | 227 | 63 | 24 | 37 | 287 | 152 | 87 | 114 | 204 | 213 |
Labelled Cell Count | 373 | 186 | 52 | 126 | 219 | 230 | 309 | 359 | 330 | 312 | 258 | 288 | 192 | 254 | 276 | 81 | 36 | 55 | 287 | 152 | 87 | 114 | 204 | 213 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.7 | 5.6 | 6.2 | 6.0 | 7.2 | 5.8 | 5.6 | 6.1 | 5.0 | 5.1 | 4.8 | 4.7 | 6.5 | 6.2 | 6.3 | 7.6 | 8.9 | 7.7 | 5.9 | 6.4 | 6.7 |
Std Deviation (1e-4) | 0.7 | 0.9 | 0.9 | 1.9 | 2.3 | 2.0 | 1.8 | 2.2 | 1.6 | 1.4 | 2.0 | 1.5 | 1.2 | 1.4 | 1.6 | 2.0 | 1.6 | 1.6 | 1.2 | 1.6 | 1.2 |
Intensity Change (Log2) | – | – | – | -0.04 | 0.21 | -0.09 | -0.15 | -0.02 | -0.33 | -0.27 | -0.38 | -0.39 | 0.06 | -0.01 | 0.03 | 0.29 | 0.52 | 0.3 | -0.07 | 0.05 | 0.11 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0.6 | 0 | 0 | 0.7 | 0 | 0.6 | 1.2 | 1.7 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 3.1 | 0 | 4.8 | 3.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.5 | 0.1 | -0.4 | 0.8 | 1.3 | 0.2 | 1.0 | -0.4 | 0.5 | -1.1 | -0.8 | 0 | -1.8 | -1.9 | -0.4 |
Nuclear Periphery | 1.3 | 0.2 | 0.6 | 0.8 | -0.8 | -0.8 | -1.2 | 0 | 0 | 1.5 | 1.8 | 0.6 | 1.7 | 2.5 | 0.1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -3.5 | -2.1 | -1.4 | -2.9 | -3.6 | -3.4 | -3.0 | -4.9 | -3.8 | -0.6 | -2.8 | -2.6 | -2.2 | -0.3 | 1.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.0165 | 0.9696 | 0.7181 | 0.3622 | 0.4742 | 0.5814 | 1.3891 | 1.5267 | 1.3889 | 1.1586 | 0.8981 | 1.342 | 1.2065 | 0.942 | 0.9717 | 0.8487 | 1.0206 | 0.7545 |
Actin | 0.0013 | 0.0008 | 0.0084 | 0.0005 | 0.005 | 0.0001 | 0.0123 | 0.0002 | 0.0002 | 0.0017 | 0.0005 | 0.0004 | 0.0086 | 0.0001 | 0.0056 | 0.0001 | 0.02 | 0.0012 |
Bud | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0004 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0015 | 0.0001 |
Bud Neck | 0.0004 | 0.0032 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0012 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0009 | 0 | 0.0004 | 0 | 0.0003 | 0.0001 |
Bud Periphery | 0.0001 | 0.0001 | 0.0005 | 0.0005 | 0.0002 | 0.0001 | 0.0007 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0 | 0.0002 | 0 | 0.005 | 0.0003 |
Bud Site | 0.0004 | 0.0027 | 0.0017 | 0.0002 | 0.0002 | 0 | 0.0046 | 0 | 0 | 0.0002 | 0.0002 | 0 | 0.0008 | 0 | 0.0057 | 0 | 0.0013 | 0.0001 |
Cell Periphery | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0.0003 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0 |
Cytoplasm | 0.0032 | 0.0031 | 0.0027 | 0.0021 | 0.0021 | 0.0007 | 0.008 | 0.0031 | 0.0007 | 0.0008 | 0.0007 | 0.001 | 0.0022 | 0.0107 | 0.0043 | 0.0001 | 0.0018 | 0.0035 |
Cytoplasmic Foci | 0.0054 | 0.0005 | 0.0029 | 0.0013 | 0.0019 | 0.0002 | 0.0138 | 0.0001 | 0.005 | 0.0058 | 0.0058 | 0.0006 | 0.0162 | 0.0001 | 0.0016 | 0 | 0.0048 | 0.0028 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0003 | 0.0001 |
Endoplasmic Reticulum | 0.0102 | 0.0116 | 0.0114 | 0.0111 | 0.0037 | 0.003 | 0.0105 | 0.0167 | 0.018 | 0.035 | 0.004 | 0.0048 | 0.0092 | 0.0179 | 0.0095 | 0.0084 | 0.005 | 0.0061 |
Endosome | 0.0255 | 0.0017 | 0.0074 | 0.0198 | 0.0144 | 0.002 | 0.0179 | 0.0011 | 0.0079 | 0.0044 | 0.0229 | 0.0037 | 0.0174 | 0.0012 | 0.0059 | 0.0018 | 0.0346 | 0.0061 |
Golgi | 0.0041 | 0.0002 | 0.0025 | 0.0025 | 0.0052 | 0.0004 | 0.0051 | 0 | 0.001 | 0.0024 | 0.0193 | 0.003 | 0.0056 | 0 | 0.0022 | 0.0001 | 0.0202 | 0.0011 |
Lipid Particles | 0.0326 | 0.0006 | 0.0056 | 0.0007 | 0.0052 | 0.0034 | 0.0169 | 0.0002 | 0.0096 | 0.0116 | 0.0028 | 0.0076 | 0.012 | 0.0002 | 0.0058 | 0.0003 | 0.0311 | 0.0032 |
Mitochondria | 0.0023 | 0.0009 | 0.0093 | 0.0149 | 0.0022 | 0.0009 | 0.0112 | 0.0006 | 0.0046 | 0.0615 | 0.0064 | 0.0131 | 0.0031 | 0.0006 | 0.006 | 0.002 | 0.0204 | 0.004 |
None | 0.0086 | 0.0011 | 0.0082 | 0.0003 | 0.027 | 0.0007 | 0.0172 | 0.0135 | 0.0038 | 0.0007 | 0.0005 | 0.0053 | 0.0016 | 0.0009 | 0.0013 | 0.0002 | 0.0089 | 0.0026 |
Nuclear Periphery | 0.8523 | 0.8766 | 0.8768 | 0.8944 | 0.7882 | 0.9399 | 0.8194 | 0.8857 | 0.8888 | 0.779 | 0.8595 | 0.9043 | 0.854 | 0.8644 | 0.8905 | 0.9382 | 0.7532 | 0.9135 |
Nucleolus | 0.0009 | 0.001 | 0.0002 | 0.0001 | 0.0014 | 0.0003 | 0.0008 | 0.0004 | 0.0002 | 0.0001 | 0.0004 | 0.0006 | 0.0014 | 0.0016 | 0.0003 | 0.0001 | 0.0008 | 0.0004 |
Nucleus | 0.0157 | 0.0528 | 0.0131 | 0.0114 | 0.1198 | 0.0297 | 0.0134 | 0.0312 | 0.0089 | 0.0153 | 0.0379 | 0.0318 | 0.0178 | 0.0675 | 0.0171 | 0.0082 | 0.0614 | 0.0327 |
Peroxisomes | 0.0025 | 0.0001 | 0.0035 | 0.0001 | 0.0008 | 0.0001 | 0.0101 | 0 | 0.0037 | 0.0445 | 0.0011 | 0.0008 | 0.011 | 0 | 0.0013 | 0 | 0.0083 | 0.001 |
Punctate Nuclear | 0.0043 | 0.0042 | 0.0015 | 0.0003 | 0.0042 | 0.0011 | 0.0026 | 0.0136 | 0.0005 | 0.0012 | 0.0209 | 0.0031 | 0.0065 | 0.0034 | 0.0009 | 0.0002 | 0.0029 | 0.0056 |
Vacuole | 0.0032 | 0.0013 | 0.0021 | 0.0041 | 0.001 | 0.0019 | 0.0045 | 0.0011 | 0.0019 | 0.002 | 0.001 | 0.002 | 0.0018 | 0.0015 | 0.0017 | 0.0008 | 0.0024 | 0.0012 |
Vacuole Periphery | 0.0268 | 0.0375 | 0.0418 | 0.0354 | 0.0168 | 0.0155 | 0.0291 | 0.0324 | 0.0449 | 0.0334 | 0.0157 | 0.0175 | 0.0295 | 0.0297 | 0.0394 | 0.0393 | 0.0157 | 0.0144 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 14.1253 | 9.7132 | 9.0095 | 14.9844 | 11.2702 | 7.0587 | 16.9412 | 14.9142 | 15.5702 | 19.4964 |
Translational Efficiency | 0.6558 | 0.8066 | 0.8895 | 0.5556 | 0.6109 | 1.2652 | 0.5009 | 0.4988 | 0.6522 | 0.5515 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
817 | 1588 | 1610 | 235 | 1962 | 2066 | 1796 | 1727 | 2779 | 3654 | 3406 | 1962 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 620.77 | 664.04 | 898.40 | 861.02 | 749.51 | 755.91 | 895.47 | 865.40 | 711.66 | 715.98 | 896.85 | 864.88 |
Standard Deviation | 68.11 | 113.66 | 126.21 | 118.31 | 87.93 | 102.09 | 111.13 | 119.62 | 101.30 | 116.54 | 118.51 | 119.47 |
Intensity Change Log 2 | — | 0.097211 | 0.533299 | 0.471988 | — | 0.012267 | 0.256697 | 0.207419 | — | 0.051369 | 0.388605 | 0.333313 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000041 | 0.000151 | 0.000221 | 0.000385 | 0.000026 | 0.000091 | 0.000111 | 0.000132 | 0.000030 | 0.000117 | 0.000163 | 0.000162 |
Bud Neck | 0.001659 | 0.001405 | 0.001761 | 0.003606 | 0.000157 | 0.001447 | 0.001003 | 0.002377 | 0.000598 | 0.001429 | 0.001361 | 0.002524 |
Bud Site | 0.000348 | 0.000508 | 0.000732 | 0.003382 | 0.000042 | 0.001789 | 0.000304 | 0.000931 | 0.000132 | 0.001232 | 0.000506 | 0.001225 |
Cell Periphery | 0.000088 | 0.000133 | 0.000040 | 0.000100 | 0.000039 | 0.000081 | 0.000032 | 0.000033 | 0.000054 | 0.000104 | 0.000036 | 0.000041 |
Cytoplasm | 0.139914* | 0.083794 | 0.023860 | 0.051889 | 0.031472 | 0.036641 | 0.026665 | 0.046931 | 0.063353 | 0.057133 | 0.025339 | 0.047525 |
Cytoplasmic Foci | 0.026012 | 0.010921 | 0.000379 | 0.006198 | 0.004607 | 0.007221 | 0.000477 | 0.001690 | 0.010900 | 0.008829 | 0.000430 | 0.002230 |
Eisosomes | 0.000030 | 0.000022 | 0.000007 | 0.000045 | 0.000008 | 0.000013 | 0.000005 | 0.000007 | 0.000015 | 0.000017 | 0.000006 | 0.000012 |
Endoplasmic Reticulum | 0.065609 | 0.039159 | 0.022862 | 0.018972 | 0.042735 | 0.047523 | 0.020628 | 0.026188 | 0.049460 | 0.043888 | 0.021684 | 0.025324 |
Endosome | 0.005283 | 0.005656 | 0.001616 | 0.006317 | 0.001665 | 0.005739 | 0.001424 | 0.003414 | 0.002728 | 0.005703 | 0.001515 | 0.003761 |
Golgi | 0.000806 | 0.000520 | 0.000032 | 0.000425 | 0.000195 | 0.001848 | 0.000031 | 0.000166 | 0.000375 | 0.001271 | 0.000031 | 0.000197 |
Lipid Particles | 0.088262 | 0.021950 | 0.000616 | 0.008080 | 0.020710 | 0.020333 | 0.000969 | 0.001625 | 0.040569 | 0.021036 | 0.000802 | 0.002398 |
Mitochondria | 0.009871 | 0.011528 | 0.002510 | 0.002301 | 0.003204 | 0.013903 | 0.001611 | 0.002465 | 0.005164 | 0.012871 | 0.002036 | 0.002445 |
Mitotic Spindle | 0.000616 | 0.001649 | 0.002944 | 0.014836 | 0.001325 | 0.004180 | 0.001127 | 0.011156 | 0.001117 | 0.003080 | 0.001986 | 0.011597 |
None | 0.015697 | 0.025564 | 0.001703 | 0.007138 | 0.002566 | 0.005041 | 0.001762 | 0.002412 | 0.006426 | 0.013960 | 0.001734 | 0.002978 |
Nuclear Periphery | 0.426756* | 0.459938* | 0.629522* | 0.299588* | 0.738054* | 0.648227* | 0.730730* | 0.658885* | 0.646535* | 0.566398* | 0.682890* | 0.615850* |
Nuclear Periphery Foci | 0.076470 | 0.095583 | 0.045633 | 0.060264 | 0.083485 | 0.075029 | 0.059274 | 0.081633 | 0.081423 | 0.083962 | 0.052826 | 0.079073 |
Nucleolus | 0.007017 | 0.028169 | 0.014664 | 0.014512 | 0.004846 | 0.008906 | 0.007737 | 0.009185 | 0.005484 | 0.017277 | 0.011012 | 0.009824 |
Nucleus | 0.124571 | 0.195283* | 0.241658* | 0.477667* | 0.057717 | 0.099982 | 0.138895* | 0.129069 | 0.077371 | 0.141399* | 0.187471* | 0.170822* |
Peroxisomes | 0.002258 | 0.000956 | 0.000174 | 0.000791 | 0.000383 | 0.000879 | 0.000115 | 0.000160 | 0.000934 | 0.000912 | 0.000143 | 0.000236 |
Vacuole | 0.007028 | 0.012698 | 0.007390 | 0.021245 | 0.004871 | 0.014065 | 0.006090 | 0.019105 | 0.005505 | 0.013471 | 0.006705 | 0.019362 |
Vacuole Periphery | 0.001662 | 0.004412 | 0.001676 | 0.002258 | 0.001894 | 0.007060 | 0.001008 | 0.002435 | 0.001826 | 0.005909 | 0.001324 | 0.002414 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -5.02 | -9.30 | -2.47 | -1.76 | -1.21 | -6.11 | -8.71 | -8.92 | -3.58 | -1.93 | -7.76 | -12.77 | -6.68 | -2.66 | -0.30 |
Bud Neck | 0.26 | -0.45 | -2.14 | -2.51 | -2.13 | -4.19 | -4.75 | -7.22 | -3.91 | -4.58 | -2.69 | -3.41 | -6.30 | -4.81 | -4.56 |
Bud Site | -0.90 | -1.20 | -1.96 | -1.86 | -1.63 | -3.06 | -2.42 | -3.48 | 0.79 | -2.47 | -3.36 | -2.16 | -3.79 | -0.71 | -2.25 |
Cell Periphery | -4.02 | 5.76 | -1.03 | 1.34 | -3.56 | -5.39 | 0.81 | 0.85 | 6.16 | -0.34 | -7.79 | 3.14 | 2.09 | 9.30 | -1.32 |
Cytoplasm | 6.30 | 14.64 | 7.35 | 3.00 | -3.83 | -1.67 | 1.09 | -5.67 | -4.24 | -6.72 | 1.46 | 11.28 | 2.22 | 1.02 | -8.35 |
Cytoplasmic Foci | 5.50 | 10.67 | 7.42 | 3.24 | -5.22 | -2.61 | 7.13 | 4.45 | 7.28 | -4.26 | 1.88 | 12.47 | 9.73 | 9.87 | -5.84 |
Eisosomes | 3.17 | 10.29 | -3.76 | -5.22 | -7.92 | -4.26 | 2.63 | -0.33 | 4.21 | -3.22 | -2.25 | 8.67 | 0.68 | 2.79 | -7.03 |
Endoplasmic Reticulum | 6.26 | 10.66 | 10.67 | 7.62 | 1.55 | -1.67 | 10.22 | 7.45 | 8.97 | -3.78 | 2.45 | 14.44 | 11.75 | 10.67 | -3.02 |
Endosome | -0.42 | 4.50 | -0.87 | -0.73 | -2.68 | -7.80 | 0.73 | -5.90 | 3.39 | -6.17 | -6.92 | 4.16 | -3.09 | 3.08 | -6.42 |
Golgi | 1.09 | 3.02 | 1.31 | 0.66 | -1.59 | -3.87 | 2.65 | 0.44 | 3.96 | -2.06 | -3.49 | 3.92 | 1.82 | 4.39 | -2.47 |
Lipid Particles | 13.17 | 18.16 | 16.14 | 7.52 | -5.75 | 0.20 | 14.18 | 13.64 | 13.43 | -3.57 | 9.40 | 21.85 | 20.85 | 18.38 | -7.25 |
Mitochondria | -0.99 | 4.99 | 4.88 | 8.66 | 0.57 | -9.03 | 4.30 | 1.63 | 9.72 | -3.13 | -8.62 | 6.15 | 4.98 | 13.20 | -1.74 |
Mitotic Spindle | -2.63 | -2.97 | -2.97 | -2.85 | -2.64 | -3.03 | -0.58 | -6.58 | -4.79 | -6.25 | -3.29 | -2.19 | -7.27 | -6.17 | -6.40 |
None | -3.20 | 5.85 | 2.43 | 5.35 | -1.63 | -3.59 | 1.89 | 0.23 | 3.49 | -1.40 | -6.18 | 6.09 | 3.86 | 9.97 | -2.15 |
Nuclear Periphery | -2.03 | -12.97 | 6.32 | 7.94 | 14.97 | 9.02 | 1.67 | 10.14 | 1.56 | 8.02 | 9.18 | -3.23 | 5.68 | -2.10 | 8.58 |
Nuclear Periphery Foci | -3.14 | 6.21 | 1.91 | 4.06 | -1.65 | 2.09 | 5.98 | 0.76 | -1.19 | -4.86 | -0.69 | 9.35 | 0.97 | 1.61 | -6.98 |
Nucleolus | -9.37 | -4.33 | -2.41 | 4.19 | 0.01 | -3.88 | -2.94 | -4.11 | -0.61 | -1.51 | -10.58 | -6.15 | -4.23 | 5.21 | 0.62 |
Nucleus | -7.30 | -11.49 | -13.31 | -10.49 | -8.43 | -7.51 | -12.97 | -13.05 | -5.83 | 0.13 | -12.59 | -19.67 | -14.88 | -5.23 | 1.43 |
Peroxisomes | 1.51 | 2.41 | 1.79 | 1.12 | -2.05 | -3.74 | 3.70 | 2.97 | 6.77 | -1.85 | -0.01 | 2.99 | 2.90 | 8.30 | -0.56 |
Vacuole | -4.09 | -0.87 | -4.81 | -4.14 | -4.67 | -7.41 | -3.11 | -9.70 | -4.57 | -7.57 | -8.82 | -3.35 | -10.58 | -6.38 | -9.04 |
Vacuole Periphery | -5.16 | -0.18 | -0.31 | 1.70 | -0.27 | -7.33 | 3.43 | -1.19 | 6.07 | -3.47 | -8.73 | 2.33 | -1.37 | 6.23 | -2.63 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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