Standard name | |
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Human Ortholog | |
Description | Sugar alcohol phosphatase; polyol phosphatase that hydrolyzes sorbitol-6-phosphate, ribitol-5-phosphate, and (D)-glycerol-3-phosphate, maintaining phosphoglucose isomerase (PGI) activity in the presence of PGI-inhibitory sugar alcohols; expression correlated with growth rate; GFP-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant displays increased glycogen accumulation; member of the haloacid dehalogenase (HAD) superfamily |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | 1 | – | 2 | 0 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 1 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 0 | 0 | 4 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 8 | 0 | 1 | 4 | 0 | 7 | – | 9 | 9 | 2 | 10 | 5 | 7 | 7 | 5 | 5 | 8 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 460 | 662 | 196 | 126 | 208 | 193 | 165 | – | 221 | 223 | 145 | 124 | 376 | 636 | 634 | 220 | 226 | 260 | 439 | 650 | 193 | 212 | 511 | 381 |
Endoplasmic Reticulum | 3 | 1 | 1 | 0 | 6 | 3 | 4 | – | 22 | 16 | 11 | 10 | 0 | 4 | 5 | 1 | 3 | 7 | 5 | 2 | 0 | 1 | 8 | 4 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 1 | 2 | 0 | 0 | – | 1 | 6 | 2 | 5 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Mitochondria | 0 | 2 | 0 | 0 | 0 | 3 | 1 | – | 6 | 2 | 8 | 6 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 |
Nucleus | 1 | 2 | 1 | 1 | 1 | 1 | 2 | – | 6 | 6 | 3 | 0 | 1 | 0 | 2 | 2 | 0 | 2 | 3 | 8 | 0 | 1 | 1 | 2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 7 | 4 | 3 | 1 | 17 | 12 | 15 | – | 12 | 27 | 17 | 25 | 0 | 0 | 0 | 1 | 1 | 0 | 3 | 8 | 0 | 1 | 3 | 4 |
Unique Cell Count | 470 | 668 | 199 | 128 | 229 | 210 | 186 | 267 | 275 | 187 | 172 | 378 | 642 | 639 | 223 | 228 | 264 | 472 | 685 | 200 | 221 | 535 | 403 | |
Labelled Cell Count | 471 | 680 | 201 | 130 | 238 | 214 | 195 | 279 | 289 | 191 | 185 | 383 | 649 | 649 | 229 | 235 | 277 | 472 | 685 | 200 | 221 | 535 | 403 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 26.6 | 23.9 | 22.3 | 22.6 | 23.6 | 15.3 | 19.6 | – | 17.6 | 17.9 | 16.1 | 17.4 | 28.0 | 27.4 | 26.6 | 33.3 | 35.8 | 35.2 | 23.7 | 24.1 | 24.2 |
Std Deviation (1e-4) | 6.2 | 5.1 | 4.1 | 4.2 | 5.6 | 3.9 | 3.6 | – | 3.7 | 3.2 | 3.5 | 3.2 | 5.4 | 4.8 | 4.7 | 8.3 | 8.8 | 8.3 | 5.8 | 5.0 | 5.4 |
Intensity Change (Log2) | – | – | – | 0.02 | 0.08 | -0.54 | -0.18 | – | -0.34 | -0.31 | -0.47 | -0.36 | 0.33 | 0.3 | 0.25 | 0.58 | 0.68 | 0.66 | 0.09 | 0.11 | 0.12 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | -3.4 | -3.1 | -4.0 | – | -5.5 | -5.8 | -6.4 | -7.4 | 1.2 | 0.7 | 0.9 | 0.1 | 0.6 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 3.8 | 3.1 | 3.0 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 2.9 | 2.3 | 3.0 | – | 1.8 | 3.7 | 3.4 | 4.7 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 13.8511 | 18.9283 | 17.6853 | 14.3537 | 14.99 | 17.8759 | 22.8567 | 27.0974 | 24.0994 | 23.1924 | 22.8144 | 25.0841 | 23.5421 | 28.7558 | 25.9465 | 25.9949 | 22.6045 | 25.5716 |
Actin | 0.0116 | 0.0001 | 0.0026 | 0.0065 | 0.0008 | 0.0001 | 0.0039 | 0.0006 | 0.0039 | 0.0021 | 0.0044 | 0.0023 | 0.0011 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0003 |
Bud | 0.0009 | 0 | 0.0002 | 0.0009 | 0.0001 | 0.0001 | 0.0061 | 0.0002 | 0.0008 | 0.0002 | 0.0002 | 0.0005 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 |
Bud Neck | 0.0017 | 0.0001 | 0.0004 | 0.0008 | 0.0004 | 0.0006 | 0.0014 | 0.0001 | 0.0013 | 0.0007 | 0.0012 | 0.001 | 0.0004 | 0.0002 | 0.0002 | 0.0003 | 0.0008 | 0.0006 |
Bud Periphery | 0.0008 | 0 | 0.0001 | 0.0005 | 0.0001 | 0 | 0.0027 | 0 | 0.0007 | 0.0004 | 0.0001 | 0.0005 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0.0031 | 0.0001 | 0.0009 | 0.0036 | 0.0003 | 0 | 0.0018 | 0.0001 | 0.002 | 0.0001 | 0.0003 | 0.0003 | 0.0002 | 0 | 0 | 0 | 0.0002 | 0 |
Cell Periphery | 0.0004 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0021 | 0 | 0.0002 | 0.0003 | 0.0003 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0 |
Cytoplasm | 0.8227 | 0.9878 | 0.9492 | 0.8366 | 0.9104 | 0.9734 | 0.8928 | 0.9877 | 0.9055 | 0.9633 | 0.9008 | 0.9633 | 0.9592 | 0.9918 | 0.9823 | 0.9912 | 0.9637 | 0.9793 |
Cytoplasmic Foci | 0.0256 | 0.0003 | 0.0066 | 0.0459 | 0.0044 | 0.0006 | 0.0087 | 0.0007 | 0.0161 | 0.0006 | 0.01 | 0.0017 | 0.0028 | 0.0003 | 0.0036 | 0.0002 | 0.0037 | 0.0008 |
Eisosomes | 0.0003 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0097 | 0.0003 | 0.0009 | 0.003 | 0.0057 | 0.0008 | 0.0039 | 0.0004 | 0.0024 | 0.005 | 0.0092 | 0.001 | 0.0028 | 0.0005 | 0.0005 | 0.0003 | 0.0019 | 0.0011 |
Endosome | 0.0328 | 0.0001 | 0.0097 | 0.0426 | 0.0161 | 0.0006 | 0.0108 | 0.0001 | 0.0162 | 0.0008 | 0.0245 | 0.003 | 0.0026 | 0.0001 | 0.001 | 0.0001 | 0.0026 | 0.0003 |
Golgi | 0.0076 | 0 | 0.0014 | 0.0108 | 0.0009 | 0 | 0.0036 | 0 | 0.0029 | 0.0004 | 0.0067 | 0.0005 | 0.0007 | 0 | 0.0002 | 0 | 0.0002 | 0 |
Lipid Particles | 0.0137 | 0 | 0.0027 | 0.0136 | 0.0024 | 0 | 0.0068 | 0 | 0.0046 | 0.0001 | 0.0021 | 0.0001 | 0.0036 | 0 | 0.0013 | 0 | 0.0005 | 0 |
Mitochondria | 0.0065 | 0 | 0.0067 | 0.0075 | 0.0011 | 0.0002 | 0.0048 | 0 | 0.0044 | 0.0066 | 0.0016 | 0.0014 | 0.0005 | 0 | 0.0001 | 0 | 0.0002 | 0 |
None | 0.0055 | 0.0002 | 0.0006 | 0.0027 | 0.0064 | 0.0003 | 0.0019 | 0.0004 | 0.0025 | 0.0006 | 0.001 | 0.0005 | 0.0017 | 0.0002 | 0.0003 | 0.0002 | 0.0026 | 0.0008 |
Nuclear Periphery | 0.0146 | 0.0011 | 0.0013 | 0.0031 | 0.0096 | 0.0029 | 0.0054 | 0.0008 | 0.0051 | 0.0021 | 0.0082 | 0.0016 | 0.0026 | 0.0006 | 0.0009 | 0.0006 | 0.0022 | 0.002 |
Nucleolus | 0.0004 | 0 | 0.0001 | 0.0003 | 0.0094 | 0 | 0.0003 | 0 | 0.0009 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0011 | 0 |
Nucleus | 0.0243 | 0.0094 | 0.0088 | 0.0098 | 0.0183 | 0.0197 | 0.0338 | 0.0082 | 0.0221 | 0.0156 | 0.0203 | 0.0197 | 0.0166 | 0.0059 | 0.0082 | 0.0067 | 0.0127 | 0.014 |
Peroxisomes | 0.0048 | 0 | 0.0049 | 0.0035 | 0.0002 | 0 | 0.0017 | 0 | 0.0022 | 0 | 0.0008 | 0.0003 | 0.0032 | 0 | 0.0007 | 0 | 0.0001 | 0 |
Punctate Nuclear | 0.0049 | 0.0001 | 0.0003 | 0.0054 | 0.0101 | 0.0002 | 0.0018 | 0.0002 | 0.002 | 0.0004 | 0.0035 | 0.0004 | 0.0009 | 0.0001 | 0.0002 | 0.0001 | 0.0055 | 0.0004 |
Vacuole | 0.0061 | 0.0001 | 0.0017 | 0.0017 | 0.0024 | 0.0005 | 0.0049 | 0.0001 | 0.0034 | 0.0005 | 0.0041 | 0.0014 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0.0013 | 0.0002 |
Vacuole Periphery | 0.002 | 0 | 0.0006 | 0.001 | 0.0009 | 0.0001 | 0.0008 | 0 | 0.0007 | 0.0001 | 0.0006 | 0.0004 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 284.9702 | 298.9019 | 288.3375 | 236.4947 | 209.8625 | 247.5969 | 282.9872 | 236.8923 | 244.8918 | 213.7221 |
Translational Efficiency | 1.4375 | 1.4521 | 1.4131 | 1.8039 | 1.8202 | 1.6417 | 1.69 | 1.9726 | 1.7502 | 1.8061 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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