Standard name | |
---|---|
Human Ortholog | |
Description | Poly (A)+ RNA-binding protein; abundant mRNP-component protein that binds mRNA and is required for stability of many mRNAs; component of glucose deprivation induced stress granules, involved in P-body-dependent granule assembly; implicated in regulation of translation; carries Q/N-rich domain at C- terminus, identified as candidate prion; human homolog Tia1 is critical for normal synaptic plasticity; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 3 | 8 | 12 | 26 | 17 | 24 | 16 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 4 | 0 | 0 | 0 | 2 | 3 | 7 | 1 | 4 | 8 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 2 | 2 | 7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 23 | 9 | 4 | 19 | 26 | 34 | 35 | 30 | 45 | 43 | 9 | 20 | 14 | 9 | 11 | 7 | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 162 | 359 | 455 | 254 | 306 | 371 | 359 | 431 | 339 | 409 | 250 | 319 | 401 | 419 | 453 | 400 | 447 | 436 | 155 | 330 | 439 | 383 | 521 | 473 |
Endoplasmic Reticulum | 0 | 6 | 12 | 5 | 12 | 29 | 60 | 108 | 114 | 104 | 71 | 100 | 5 | 2 | 2 | 16 | 14 | 15 | 0 | 3 | 19 | 2 | 2 | 6 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 1 | 2 | 0 | 0 | 2 | 7 | 7 | 2 | 5 | 1 | 0 | 0 | 0 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2 | 0 | 0 | 0 | 1 | 9 | 18 | 15 | 27 | 30 | 24 | 5 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 4 | 0 | 0 | 2 | 2 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 0 | 3 | 2 | 0 | 1 | 0 | 0 | 11 | 12 | 4 | 10 | 22 | 1 | 1 | 2 | 4 | 3 | 5 | 0 | 7 | 2 | 1 | 6 | 3 |
Unique Cell Count | 164 | 368 | 468 | 265 | 319 | 418 | 451 | 576 | 503 | 565 | 381 | 462 | 404 | 421 | 454 | 410 | 456 | 443 | 164 | 356 | 469 | 394 | 540 | 501 |
Labelled Cell Count | 164 | 374 | 493 | 271 | 326 | 433 | 477 | 625 | 562 | 603 | 439 | 512 | 416 | 442 | 471 | 434 | 478 | 468 | 164 | 356 | 469 | 394 | 540 | 501 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 49.1 | 49.0 | 47.9 | 42.8 | 40.9 | 37.5 | 34.6 | 33.9 | 33.2 | 31.2 | 32.9 | 31.7 | 56.8 | 53.6 | 52.4 | 67.9 | 59.7 | 67.3 | 56.2 | 57.6 | 64.0 |
Std Deviation (1e-4) | 11.6 | 12.4 | 9.6 | 9.1 | 8.0 | 7.1 | 7.8 | 8.2 | 7.7 | 7.6 | 9.4 | 7.9 | 12.6 | 12.0 | 10.4 | 23.7 | 18.2 | 23.2 | 13.8 | 14.1 | 16.2 |
Intensity Change (Log2) | – | – | – | -0.16 | -0.23 | -0.35 | -0.47 | -0.5 | -0.53 | -0.62 | -0.54 | -0.6 | 0.24 | 0.16 | 0.13 | 0.5 | 0.32 | 0.49 | 0.23 | 0.26 | 0.42 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 3.1 | 5.0 | 3.8 | 5.5 | 4.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.0 | -2.8 | -0.3 | 0.6 | 0.7 | 1.3 | 0.3 | 3.7 | 2.6 | -2.1 | -0.1 | -1.4 | -2.1 | -2.0 | -2.8 |
Cytoplasm | -1.0 | -1.0 | -5.0 | -8.4 | -10.0 | -12.0 | -10.7 | -12.2 | -11.5 | 2.2 | 2.7 | 3.2 | 0.3 | 0.8 | 1.2 |
Endoplasmic Reticulum | -0.6 | 1.0 | 3.1 | 6.1 | 8.2 | 9.3 | 8.0 | 7.8 | 8.9 | -1.4 | -2.5 | -2.6 | 1.1 | 0.5 | 0.7 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 4.4 | 3.5 | 5.1 | 5.1 | 5.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 2.1 | 2.6 | 0 | 2.7 | 4.2 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 50.6991 | 58.9833 | 56.699 | 54.0101 | 51.3832 | 55.8568 | 51.2429 | 67.6896 | 62.0342 | 61.0028 | 54.4925 | 60.5796 | 57.851 | 67.4197 | 62.2075 | 55.2144 | 54.5113 | 61.297 |
Actin | 0.0026 | 0.0009 | 0.0002 | 0.0003 | 0.0001 | 0.0017 | 0.0118 | 0.0039 | 0.0043 | 0.0002 | 0.0366 | 0.0059 | 0.0147 | 0.0004 | 0.0005 | 0.0422 | 0.0001 | 0.0003 |
Bud | 0.0004 | 0.0001 | 0.0003 | 0.0004 | 0.0001 | 0.0007 | 0.0017 | 0.0001 | 0.0005 | 0.0001 | 0.0006 | 0.0005 | 0.0004 | 0.0001 | 0.0002 | 0.0005 | 0.0002 | 0.0002 |
Bud Neck | 0.0004 | 0.0002 | 0.0003 | 0.0003 | 0.0003 | 0.0009 | 0.0009 | 0.0001 | 0.0005 | 0.0003 | 0.0004 | 0.0012 | 0.0012 | 0.0002 | 0.0003 | 0.0035 | 0.0005 | 0.0013 |
Bud Periphery | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0015 | 0.0001 | 0.0006 | 0 | 0.0005 | 0.0004 | 0.0004 | 0.0001 | 0.0003 | 0.0005 | 0.0002 | 0.0003 |
Bud Site | 0.0003 | 0.0008 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0019 | 0.0008 | 0.0053 | 0 | 0.0006 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0.0007 | 0.0001 | 0.0001 |
Cell Periphery | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0011 | 0.0002 | 0.001 | 0.0001 | 0.0004 | 0.0007 | 0.0014 | 0.0004 | 0.0015 | 0.0003 | 0.0008 | 0.0004 |
Cytoplasm | 0.8669 | 0.9924 | 0.9432 | 0.9275 | 0.9542 | 0.9272 | 0.8391 | 0.9854 | 0.9375 | 0.9646 | 0.8696 | 0.9221 | 0.8476 | 0.9822 | 0.9448 | 0.8764 | 0.9145 | 0.9247 |
Cytoplasmic Foci | 0.0117 | 0.0007 | 0.0045 | 0.0011 | 0.0032 | 0.0068 | 0.0147 | 0.0004 | 0.0044 | 0.0007 | 0.0078 | 0.0016 | 0.0033 | 0.0008 | 0.0045 | 0.0064 | 0.0041 | 0.0017 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0002 | 0 | 0 | 0.0008 | 0 | 0 |
Endoplasmic Reticulum | 0.0149 | 0.0009 | 0.0181 | 0.0086 | 0.0115 | 0.0245 | 0.0262 | 0.0054 | 0.0044 | 0.0144 | 0.0056 | 0.02 | 0.0564 | 0.0084 | 0.0175 | 0.0023 | 0.0081 | 0.0343 |
Endosome | 0.0141 | 0.0003 | 0.0047 | 0.0062 | 0.0114 | 0.0077 | 0.014 | 0.0002 | 0.0034 | 0.0013 | 0.0154 | 0.0049 | 0.0093 | 0.0008 | 0.003 | 0.0047 | 0.0172 | 0.0044 |
Golgi | 0.0013 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0011 | 0.0026 | 0.0001 | 0.0012 | 0 | 0.0185 | 0.0012 | 0.0018 | 0.0002 | 0.0005 | 0.0034 | 0.0011 | 0.0002 |
Lipid Particles | 0.0047 | 0.0001 | 0.0003 | 0 | 0.0002 | 0.0006 | 0.0082 | 0 | 0.0032 | 0.0001 | 0.0116 | 0.0008 | 0.0025 | 0.0001 | 0.005 | 0.0154 | 0.0007 | 0.0001 |
Mitochondria | 0.0014 | 0 | 0.0006 | 0.0002 | 0.0003 | 0.0013 | 0.0079 | 0.0001 | 0.0132 | 0.0001 | 0.0068 | 0.0017 | 0.0022 | 0.0008 | 0.0036 | 0.0201 | 0.0074 | 0.0007 |
None | 0.0071 | 0.0001 | 0.0009 | 0.0002 | 0.0013 | 0.0011 | 0.0056 | 0.0001 | 0.0019 | 0.0001 | 0.0035 | 0.0004 | 0.0024 | 0.0004 | 0.0014 | 0.0022 | 0.0021 | 0.0002 |
Nuclear Periphery | 0.0198 | 0.0007 | 0.0064 | 0.0086 | 0.0031 | 0.0078 | 0.0167 | 0.0011 | 0.0032 | 0.008 | 0.0054 | 0.0177 | 0.0172 | 0.001 | 0.0056 | 0.005 | 0.0034 | 0.0066 |
Nucleolus | 0.0005 | 0 | 0.0006 | 0 | 0 | 0.0014 | 0.0032 | 0 | 0.0009 | 0 | 0.0004 | 0.0003 | 0.0009 | 0 | 0.0012 | 0.0026 | 0.0003 | 0.0001 |
Nucleus | 0.0106 | 0.0009 | 0.0028 | 0.0035 | 0.0017 | 0.0037 | 0.0134 | 0.0007 | 0.0026 | 0.003 | 0.0027 | 0.0042 | 0.0068 | 0.0011 | 0.0021 | 0.0025 | 0.0029 | 0.0024 |
Peroxisomes | 0.0014 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.001 | 0.0002 | 0.0006 | 0 | 0.0021 | 0.0002 | 0.0047 | 0 | 0.0007 | 0.0013 | 0 | 0 |
Punctate Nuclear | 0.006 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0017 | 0.007 | 0 | 0.0002 | 0.0001 | 0.0019 | 0.0012 | 0.0011 | 0 | 0.0002 | 0.0018 | 0.0001 | 0 |
Vacuole | 0.0269 | 0.0012 | 0.0129 | 0.031 | 0.0095 | 0.008 | 0.0165 | 0.0011 | 0.0082 | 0.006 | 0.0071 | 0.0104 | 0.0187 | 0.0025 | 0.0055 | 0.0039 | 0.0234 | 0.0114 |
Vacuole Periphery | 0.0083 | 0.0001 | 0.0031 | 0.0112 | 0.0023 | 0.003 | 0.0046 | 0.0001 | 0.0025 | 0.0008 | 0.002 | 0.0045 | 0.0063 | 0.0003 | 0.0015 | 0.0034 | 0.0129 | 0.0105 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 233.319 | 220.8845 | 161.2189 | 234.4357 | 215.8523 | 172.4633 | 216.0136 | 206.5836 | 187.6793 | 232.5825 |
Translational Efficiency | 2.1949 | 2.1937 | 2.5844 | 1.408 | 2.1793 | 3.2271 | 2.0573 | 1.908 | 2.1285 | 2.0634 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1481 | 471 | 2761 | 1891 | 2177 | 1911 | 1375 | 1047 | 3658 | 2382 | 4136 | 2938 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 2505.89 | 3365.56 | 4657.25 | 5705.63 | 3497.91 | 3591.10 | 4770.50 | 5414.91 | 3096.27 | 3546.50 | 4694.90 | 5602.03 |
Standard Deviation | 579.10 | 827.23 | 858.74 | 1093.18 | 605.23 | 680.76 | 840.11 | 1317.96 | 768.70 | 717.76 | 854.26 | 1186.41 |
Intensity Change Log 2 | — | 0.425523 | 0.894155 | 1.187063 | — | 0.037933 | 0.447647 | 0.630444 | — | 0.212519 | 0.651038 | 0.889279 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000137 | 0.000129 | 0.000071 | 0.000190 | 0.000026 | 0.000127 | 0.000112 | 0.000626 | 0.000071 | 0.000127 | 0.000084 | 0.000345 |
Bud Neck | 0.000238 | 0.000694 | 0.000489 | 0.001017 | 0.000139 | 0.000426 | 0.002278 | 0.003169 | 0.000179 | 0.000479 | 0.001084 | 0.001784 |
Bud Site | 0.005629 | 0.006202 | 0.003123 | 0.005370 | 0.000944 | 0.002835 | 0.002353 | 0.013538 | 0.002841 | 0.003501 | 0.002867 | 0.008281 |
Cell Periphery | 0.004487 | 0.009879 | 0.004196 | 0.019551 | 0.003450 | 0.004325 | 0.009788 | 0.019801 | 0.003870 | 0.005423 | 0.006055 | 0.019640 |
Cytoplasm | 0.629637* | 0.792186* | 0.806865* | 0.805426* | 0.822503* | 0.862038* | 0.778139* | 0.699985* | 0.744418* | 0.848226* | 0.797315* | 0.767850* |
Cytoplasmic Foci | 0.043208 | 0.010622 | 0.004111 | 0.002142 | 0.008099 | 0.008528 | 0.004978 | 0.007733 | 0.022314 | 0.008942 | 0.004399 | 0.004135 |
Eisosomes | 0.000001 | 0.000002 | 0.000001 | 0.000003 | 0.000000 | 0.000001 | 0.000002 | 0.000009 | 0.000001 | 0.000001 | 0.000001 | 0.000005 |
Endoplasmic Reticulum | 0.004290 | 0.001306 | 0.000764 | 0.000168 | 0.002394 | 0.001247 | 0.000282 | 0.000224 | 0.003162 | 0.001259 | 0.000603 | 0.000188 |
Endosome | 0.002223 | 0.001353 | 0.000653 | 0.000447 | 0.000624 | 0.001044 | 0.000585 | 0.003454 | 0.001271 | 0.001105 | 0.000631 | 0.001518 |
Golgi | 0.114145* | 0.048278 | 0.011348 | 0.005259 | 0.042809 | 0.037070 | 0.012140 | 0.007653 | 0.071691 | 0.039286 | 0.011611 | 0.006112 |
Lipid Particles | 0.004857 | 0.013490 | 0.006784 | 0.017325 | 0.004723 | 0.007406 | 0.013043 | 0.034245 | 0.004777 | 0.008609 | 0.008865 | 0.023355 |
Mitochondria | 0.000716 | 0.000343 | 0.000250 | 0.000190 | 0.000107 | 0.000130 | 0.000130 | 0.001043 | 0.000353 | 0.000172 | 0.000210 | 0.000494 |
Mitotic Spindle | 0.003335 | 0.001597 | 0.000508 | 0.000251 | 0.000812 | 0.001510 | 0.000912 | 0.005067 | 0.001833 | 0.001527 | 0.000643 | 0.001967 |
None | 0.001948 | 0.002934 | 0.000607 | 0.001123 | 0.001156 | 0.001114 | 0.001805 | 0.001744 | 0.001477 | 0.001474 | 0.001005 | 0.001344 |
Nuclear Periphery | 0.000259 | 0.000313 | 0.000261 | 0.000203 | 0.000378 | 0.000231 | 0.001084 | 0.000351 | 0.000330 | 0.000247 | 0.000535 | 0.000255 |
Nuclear Periphery Foci | 0.008574 | 0.016284 | 0.006328 | 0.003441 | 0.012495 | 0.011249 | 0.013744 | 0.005820 | 0.010908 | 0.012244 | 0.008794 | 0.004289 |
Nucleolus | 0.000051 | 0.000085 | 0.000077 | 0.000099 | 0.000028 | 0.000043 | 0.000113 | 0.000175 | 0.000037 | 0.000051 | 0.000089 | 0.000126 |
Nucleus | 0.003963 | 0.011934 | 0.004578 | 0.014418 | 0.001893 | 0.003520 | 0.033436 | 0.018258 | 0.002731 | 0.005184 | 0.014172 | 0.015786 |
Peroxisomes | 0.000030 | 0.000049 | 0.000046 | 0.000049 | 0.000009 | 0.000019 | 0.000012 | 0.000120 | 0.000017 | 0.000025 | 0.000035 | 0.000074 |
Vacuole | 0.031191 | 0.057241 | 0.120857 | 0.117246 | 0.033117 | 0.034749 | 0.113077 | 0.171545 | 0.032337 | 0.039197 | 0.118271 | 0.136597 |
Vacuole Periphery | 0.141081* | 0.025082 | 0.028081 | 0.006083 | 0.064293 | 0.022388 | 0.011987 | 0.005443 | 0.095382 | 0.022921 | 0.022731 | 0.005855 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.33 | 1.34 | -1.22 | -1.93 | -3.33 | -1.99 | -11.49 | -3.87 | -2.95 | -2.99 | -1.20 | -1.41 | -4.70 | -3.07 | -4.12 |
Bud Neck | -4.90 | -6.57 | -10.52 | -4.04 | -6.12 | -6.00 | -11.68 | -10.29 | -9.28 | -0.48 | -7.05 | -13.37 | -14.02 | -11.14 | -3.28 |
Bud Site | -0.34 | 3.69 | -0.06 | 0.30 | -3.34 | -4.63 | -5.89 | -8.23 | -6.60 | -6.78 | -1.60 | -1.34 | -8.02 | -5.97 | -7.34 |
Cell Periphery | -4.20 | 0.32 | -11.16 | -5.62 | -12.35 | -2.22 | -8.09 | -10.37 | -9.50 | -5.18 | -3.47 | -4.79 | -15.87 | -13.73 | -13.24 |
Cytoplasm | -10.38 | -16.45 | -14.94 | 0.11 | 1.17 | -4.84 | 8.45 | 13.76 | 17.47 | 5.79 | -14.20 | -5.20 | -0.39 | 13.85 | 4.79 |
Cytoplasmic Foci | 14.09 | 19.34 | 20.15 | 7.01 | 5.86 | -0.72 | 2.21 | -0.64 | -0.13 | -2.43 | 12.88 | 18.11 | 17.59 | 7.80 | 0.34 |
Eisosomes | -1.44 | 0.79 | -6.98 | -4.00 | -7.22 | -3.37 | -10.84 | -5.58 | -5.15 | -4.09 | -2.52 | -5.87 | -8.30 | -7.36 | -6.76 |
Endoplasmic Reticulum | 4.41 | 5.84 | 6.87 | 3.87 | 8.74 | 2.81 | 6.30 | 6.63 | 4.43 | 2.31 | 5.11 | 8.07 | 9.52 | 5.46 | 8.98 |
Endosome | 3.98 | 12.61 | 8.68 | 3.68 | 1.48 | -3.32 | -0.82 | -3.17 | -2.43 | -3.04 | 1.19 | 8.90 | 0.21 | -0.33 | -2.06 |
Golgi | 11.10 | 24.03 | 25.59 | 10.24 | 11.03 | 2.23 | 16.46 | 19.31 | 16.29 | 6.05 | 12.10 | 28.22 | 31.17 | 20.11 | 12.32 |
Lipid Particles | -5.40 | -3.19 | -10.15 | -2.27 | -8.74 | -3.77 | -7.99 | -13.12 | -11.73 | -8.01 | -5.96 | -8.00 | -16.58 | -12.31 | -11.91 |
Mitochondria | 1.32 | 2.50 | 2.77 | 0.63 | 0.82 | -1.56 | -3.10 | -2.58 | -2.49 | -2.40 | 2.12 | 1.64 | -0.43 | -2.00 | -1.63 |
Mitotic Spindle | 3.60 | 8.91 | 10.00 | 3.65 | 4.82 | -3.33 | -1.52 | -2.66 | -2.09 | -2.40 | 1.27 | 7.02 | 0.26 | -0.31 | -1.99 |
None | -1.23 | 3.73 | 2.23 | 2.59 | -3.96 | 0.30 | -4.38 | -1.76 | -1.85 | 0.53 | 0.04 | 3.02 | 0.95 | 0.89 | -2.28 |
Nuclear Periphery | -0.96 | -0.55 | 0.96 | 2.11 | 1.81 | 4.43 | -5.36 | 0.31 | -3.30 | 5.40 | 2.69 | -4.30 | 2.22 | -0.75 | 5.52 |
Nuclear Periphery Foci | -3.74 | 2.29 | 7.41 | 6.34 | 7.05 | 1.05 | -1.71 | 7.96 | 6.08 | 8.04 | -1.60 | 2.22 | 11.92 | 10.13 | 10.46 |
Nucleolus | -2.28 | -3.50 | -5.49 | -1.15 | -3.48 | -1.52 | -10.37 | -6.64 | -5.19 | -1.55 | -1.58 | -11.86 | -9.82 | -5.70 | -2.98 |
Nucleus | -2.97 | -1.60 | -10.75 | -1.91 | -12.42 | -5.31 | -19.56 | -15.72 | -14.43 | 6.74 | -3.74 | -17.55 | -20.03 | -15.30 | -2.51 |
Peroxisomes | -1.49 | -2.50 | -2.52 | -0.01 | -0.45 | -3.40 | -2.86 | -5.14 | -4.50 | -4.84 | -2.05 | -5.08 | -6.49 | -5.18 | -4.07 |
Vacuole | -4.86 | -23.33 | -21.32 | -10.15 | -0.36 | -1.05 | -17.71 | -23.13 | -22.30 | -7.12 | -3.46 | -29.37 | -30.89 | -26.53 | -4.84 |
Vacuole Periphery | 19.64* | 20.99 | 25.56* | 7.11 | 18.37* | 13.44 | 18.29 | 21.29 | 11.80 | 7.02 | 23.79 | 25.35 | 32.36 | 13.54 | 19.51* |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|