Standard name
Human Ortholog
Description Transcription factor, activates transcription of stress response genes; nuclear localization is positively regulated by calcineurin-mediated dephosphorylation; rapidly localizes to the nucleus under blue light stress; can be activated in stochastic pulses of nuclear localization in response to calcium

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.1 0.1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.05 0.15 0.05 0.12 0.09 0.08 0.07 0.05 0.06 0.07 0.08 0.06 0.06 0 0.15 0.12 0.17 0 0 0 0 0 0
Cytoplasm 0.95 0.94 0.95 0.88 0.9 0.85 0.83 0.79 0.8 0.7 0.7 0.99 0.98 0.96 0.88 0.84 0.86 0.92 0.91 0.84 0.6 0.5 0.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.07 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.06 0.06 0.1 0.12 0.29 0.27 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.05 0 0 0 0.19 0.2 0.16
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Vac/Vac Membrane 0 0 0 0 0.07 0 0 0 0.07 0.08 0.07 0 0 0 0.07 0.11 0.1 0 0 0 0 0.06 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 1 3 0 3 2 2 4 0 0 0 0 0 0 0 0 1 3 0 0 1
Bud 4 0 0 0 1 1 4 1 4 11 5 0 0 0 0 0 0 0 0 0 6 24 29
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 3 1 4 6
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 14 41 10 28 22 27 27 16 16 20 24 14 18 14 53 49 68 0 1 1 0 0 0
Cytoplasm 254 251 201 204 212 271 322 232 205 202 207 248 299 330 314 349 350 242 249 186 74 120 153
Endoplasmic Reticulum 4 11 6 9 7 13 17 19 11 14 4 0 2 2 7 16 11 5 7 8 0 1 1
Endosome 0 0 0 0 0 1 3 0 0 1 0 0 0 2 5 3 4 0 1 1 1 2 6
Golgi 1 2 0 0 0 0 0 1 0 0 0 0 2 0 2 2 3 2 2 0 0 0 1
Mitochondria 1 0 0 1 6 19 25 30 30 84 79 0 2 2 0 5 3 1 2 2 1 1 2
Nucleus 4 2 1 7 0 6 8 5 4 2 3 2 1 5 17 23 21 2 0 1 23 49 49
Nuclear Periphery 1 0 0 0 0 0 0 0 0 3 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 1 1 0 0 1 1 2 0 1 1 0 0 1 5 2 2 0 0 0 4 12 17
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 1 0 0 0 3 6 15
Vac/Vac Membrane 2 1 2 9 17 14 16 13 19 24 20 1 2 7 26 46 39 2 1 2 4 13 18
Unique Cell Count 266 267 211 231 236 319 387 292 257 288 294 250 305 344 356 417 407 265 273 221 124 243 308
Labelled Cell Count 285 309 222 259 268 353 426 321 291 366 345 265 326 364 430 495 502 265 273 221 124 243 308


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.6 10.3 9.0 8.6 9.3 7.6 7.8 6.8 7.0 6.6 6.5 9.1 9.5 9.4 9.5 9.5 9.5 8.3 8.4 8.4
Std Deviation (1e-4) 1.0 1.2 1.4 1.5 2.0 1.7 1.6 1.3 1.5 1.9 1.3 1.2 1.2 1.2 1.2 1.3 1.3 0.9 1.2 1.4
Intensity Change (Log2) -0.06 0.05 -0.24 -0.19 -0.41 -0.36 -0.45 -0.46 0.03 0.09 0.07 0.08 0.09 0.07 -0.11 -0.09 -0.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2.8 1.9 1.6 1.1 0.4 0.7 1.0 1.5 0.4 0.6 -0.4 3.7 2.8 4.2
Cytoplasm -2.6 -2.2 -3.7 -4.3 -5.1 -4.9 -7.0 -7.0 2.6 1.8 0.4 -2.8 -4.2 -3.5
Endoplasmic Reticulum 0.6 0.1 0.7 0.9 1.9 0.8 1.1 0 0 0 0 0 0.6 -0.1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.6 3.8 4.8 5.1 8.6 8.2 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 2.8 3.1 3.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.0 3.3 2.3 2.2 2.3 3.4 3.7 3.2 0 0 0 3.4 4.5 4.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.8915 2.7692 2.3414 2.3189 2.434 2.3608 1.6034 2.957 2.1317 2.3341 0.9467 2.5419 3.3481 4.047 3.6579 3.1864 3.5207 3.5571
Actin 0.0422 0.0025 0.0044 0.0159 0.0059 0.0006 0.0108 0.0023 0.0112 0.0006 0.0002 0.0058 0.0339 0.0028 0.0006 0.0081 0.0169 0.0076
Bud 0.0004 0.0007 0.0007 0.0002 0.0004 0.0001 0.0016 0.0022 0.0004 0.0003 0.0002 0.0001 0.0102 0.0012 0.0033 0.0002 0.0013 0.0012
Bud Neck 0.0007 0.0013 0.0018 0.0061 0.0008 0.0009 0.0013 0.0029 0.003 0.0003 0.0007 0.0012 0.005 0.0006 0.0009 0.0005 0.0074 0.0072
Bud Periphery 0.0004 0.0005 0.0007 0.0002 0.0003 0.0001 0.0018 0.0012 0.0006 0.0005 0.0001 0.0002 0.0091 0.0007 0.0016 0.0002 0.0011 0.0013
Bud Site 0.0029 0.0325 0.014 0.0011 0.0022 0.0003 0.0062 0.0183 0.0022 0.0002 0.0032 0.0001 0.0199 0.0135 0.0078 0.0004 0.0004 0.0009
Cell Periphery 0.001 0.0018 0.0012 0.0003 0.0002 0.0001 0.0011 0.002 0.001 0.0008 0.0002 0.0003 0.0022 0.0006 0.0006 0.0002 0.0005 0.0003
Cytoplasm 0.7586 0.8743 0.9021 0.8324 0.7073 0.9196 0.7438 0.8567 0.7568 0.8223 0.9484 0.8525 0.7719 0.9134 0.9002 0.9401 0.8888 0.8637
Cytoplasmic Foci 0.0272 0.006 0.0083 0.0369 0.1216 0.0053 0.0144 0.0048 0.0185 0.0044 0.0079 0.0067 0.0169 0.0065 0.0063 0.0086 0.005 0.0088
Eisosomes 0.0003 0.0001 0.0001 0.0001 0.0001 0 0.0003 0.0003 0.0007 0.0001 0 0.0001 0.0003 0 0 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0332 0.0067 0.0142 0.0112 0.0032 0.0081 0.0156 0.0076 0.0116 0.0026 0.0023 0.0051 0.0205 0.0119 0.0089 0.014 0.0046 0.0085
Endosome 0.0352 0.0054 0.0052 0.0107 0.04 0.0232 0.0136 0.0026 0.0157 0.0029 0.0029 0.0045 0.021 0.0053 0.0038 0.0062 0.0069 0.0126
Golgi 0.0148 0.001 0.0023 0.018 0.0355 0.0033 0.0069 0.0004 0.0103 0.0002 0.0003 0.002 0.0053 0.001 0.0005 0.0014 0.0025 0.0032
Lipid Particles 0.0232 0.0004 0.0016 0.0354 0.0164 0.0015 0.0141 0.0003 0.007 0.0001 0.0001 0.002 0.0042 0.0006 0.0002 0.0007 0.0011 0.0024
Mitochondria 0.0073 0.0006 0.0028 0.0013 0.0498 0.001 0.0125 0.0001 0.0072 0.0001 0.0001 0.0004 0.0015 0.0005 0.0001 0.0002 0.0002 0.0011
None 0.02 0.0367 0.034 0.0025 0.001 0.0317 0.1231 0.0948 0.1274 0.1603 0.0305 0.113 0.045 0.0149 0.034 0.0151 0.0038 0.0225
Nuclear Periphery 0.0032 0.0012 0.0009 0.0009 0.0003 0.0009 0.0013 0.0005 0.0013 0.0004 0.0003 0.0004 0.0056 0.001 0.0005 0.0008 0.0018 0.0023
Nucleolus 0.0003 0.0004 0.0001 0.0001 0.0002 0.0001 0.0009 0.0001 0.0018 0.0001 0.0001 0 0.002 0.0004 0.0002 0 0.0005 0.0054
Nucleus 0.0012 0.0251 0.0008 0.0007 0.0003 0.0007 0.0119 0.0011 0.0019 0.0009 0.0009 0.0038 0.009 0.022 0.0285 0.001 0.0445 0.0455
Peroxisomes 0.0201 0.0003 0.0014 0.0225 0.0113 0.0002 0.012 0.0001 0.0171 0 0.0001 0.0008 0.003 0.0002 0.0001 0.0004 0.0003 0.001
Punctate Nuclear 0.0029 0.001 0.0004 0.001 0.0009 0.0005 0.0029 0.0004 0.0022 0.0002 0.0004 0.0002 0.0079 0.0009 0.0003 0.0005 0.0035 0.0018
Vacuole 0.0039 0.0014 0.0028 0.0024 0.0017 0.0014 0.0035 0.0013 0.0017 0.0023 0.0012 0.0008 0.005 0.002 0.0013 0.0013 0.0082 0.0023
Vacuole Periphery 0.0011 0.0001 0.0003 0.0002 0.0008 0.0004 0.0004 0.0001 0.0004 0.0002 0.0001 0.0001 0.0006 0.0002 0.0001 0.0002 0.0004 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.4172 14.5269 9.7016 17.0572 9.8324 12.9931 18.4826 22.801 15.0068 18.4022
Translational Efficiency 1.1853 1.5924 2.096 1.0353 1.958 1.9729 1.4301 0.973 1.5403 1.3115

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Transcription factor, activates transcription of stress response genes; nuclear localization is positively regulated by calcineurin-mediated dephosphorylation; rapidly localizes to the nucleus under blue light stress; can be activated in stochastic pulses of nuclear localization in response to calcium
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Crz1

Crz1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Crz1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available