Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase; complex catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 3 | 2 | 2 | 1 | 0 | 1 | 1 | 2 | 1 | 2 | 2 | 4 | 0 | 0 | 2 | 4 | 5 | 4 | 4 | 13 | 20 | 25 |
Bud | 5 | 11 | 16 | 8 | 5 | 10 | 23 | 22 | 18 | 22 | 18 | 14 | 2 | 4 | 0 | 16 | 4 | 13 | 0 | 3 | 0 | 5 | 6 | 15 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 10 |
Bud Site | 2 | 1 | 2 | 2 | 0 | 2 | 10 | 3 | 14 | 6 | 3 | 3 | 0 | 0 | 0 | 3 | 1 | 3 | – | – | – | – | – | – |
Cell Periphery | 4 | 12 | 14 | 9 | 4 | 6 | 4 | 1 | 4 | 3 | 0 | 1 | 9 | 8 | 11 | 30 | 13 | 18 | 0 | 0 | 0 | 0 | 0 | 3 |
Cytoplasm | 4 | 6 | 5 | 3 | 8 | 11 | 9 | 11 | 6 | 9 | 6 | 6 | 5 | 7 | 6 | 18 | 8 | 11 | 0 | 1 | 1 | 0 | 0 | 0 |
Endoplasmic Reticulum | 3 | 0 | 2 | 1 | 2 | 2 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 6 | 7 | 7 | 0 | 1 | 0 | 1 | 1 | 2 |
Endosome | 7 | 23 | 19 | 14 | 28 | 15 | 13 | 20 | 2 | 3 | 0 | 2 | 38 | 41 | 24 | 38 | 19 | 22 | 23 | 19 | 19 | 32 | 27 | 42 |
Golgi | 0 | 1 | 14 | 20 | 18 | 1 | 4 | 5 | 0 | 3 | 0 | 6 | 47 | 91 | 116 | 27 | 23 | 19 | 15 | 36 | 13 | 23 | 28 | 34 |
Mitochondria | 331 | 339 | 195 | 235 | 251 | 410 | 531 | 360 | 385 | 322 | 280 | 246 | 192 | 221 | 232 | 14 | 8 | 9 | 275 | 277 | 225 | 197 | 210 | 303 |
Nucleus | 2 | 3 | 1 | 0 | 3 | 3 | 6 | 10 | 2 | 4 | 2 | 3 | 2 | 0 | 0 | 8 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 11 | 6 | 9 | 3 | 17 | 35 | 26 | 27 | 23 | 19 | 24 | 14 | 5 | 5 | 1 | 3 | 4 | 0 | 3 | 2 | 3 | 3 | 4 | 2 |
Nucleolus | 13 | 12 | 10 | 5 | 19 | 38 | 41 | 36 | 35 | 37 | 41 | 20 | 9 | 8 | 11 | 6 | 3 | 2 | 1 | 3 | 0 | 5 | 4 | 3 |
Peroxisomes | 18 | 22 | 45 | 40 | 28 | 19 | 21 | 24 | 14 | 12 | 3 | 6 | 48 | 85 | 94 | 124 | 45 | 89 | 24 | 13 | 39 | 35 | 65 | 74 |
SpindlePole | 14 | 7 | 31 | 16 | 4 | 16 | 29 | 20 | 12 | 17 | 8 | 6 | 18 | 13 | 13 | 36 | 15 | 23 | 2 | 1 | 2 | 11 | 10 | 16 |
Vac/Vac Membrane | 31 | 70 | 23 | 12 | 32 | 16 | 24 | 13 | 11 | 8 | 4 | 3 | 27 | 36 | 32 | 31 | 14 | 16 | 5 | 24 | 2 | 5 | 6 | 7 |
Unique Cell Count | 357 | 387 | 279 | 266 | 333 | 479 | 586 | 434 | 427 | 370 | 313 | 275 | 276 | 372 | 392 | 279 | 129 | 187 | 363 | 394 | 317 | 344 | 393 | 551 |
Labelled Cell Count | 445 | 514 | 389 | 370 | 421 | 586 | 742 | 553 | 529 | 468 | 392 | 332 | 404 | 524 | 540 | 360 | 168 | 236 | 363 | 394 | 317 | 344 | 393 | 551 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.9 | 8.2 | 7.4 | 8.3 | 10.0 | 8.8 | 7.8 | 8.2 | 8.2 | 10.1 | 10.6 | 13.2 | 8.3 | 10.8 | 14.0 | 8.4 | 8.5 | 8.1 | 12.2 | 10.6 | 11.5 |
Std Deviation (1e-4) | 2.2 | 4.1 | 3.9 | 4.1 | 3.2 | 3.8 | 3.4 | 3.4 | 4.5 | 5.8 | 6.4 | 7.6 | 2.3 | 3.7 | 6.1 | 3.0 | 2.9 | 2.5 | 16.9 | 12.9 | 14.8 |
Intensity Change (Log2) | – | – | – | 0.18 | 0.44 | 0.25 | 0.08 | 0.16 | 0.16 | 0.46 | 0.52 | 0.85 | 0.18 | 0.56 | 0.93 | 0.19 | 0.21 | 0.13 | 0.73 | 0.53 | 0.65 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | -1.6 | -2.9 | -2.7 | -1.2 | -0.4 | -0.9 | 0.1 | 0 | -0.3 | -3.3 | -3.4 | -4.8 | 0 | -1.1 | 0.5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 2.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.9 | -2.8 | -3.1 | -4.2 | -4.3 | -3.4 | -3.3 | -4.0 | -3.4 | -1.0 | -2.0 | -1.5 | 2.5 | 1.9 | 1.9 |
Cytoplasm | 0 | 0.5 | 0.5 | 0 | 0.7 | 0 | 0.6 | 0.1 | 0.3 | 0 | 0.1 | 0 | 2.8 | 0 | 2.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -0.8 | 0.7 | -2.4 | -3.3 | -1.3 | -4.8 | -4.2 | -4.7 | -3.7 | 2.7 | 1.8 | -0.4 | 2.7 | 2.6 | 1.9 |
Golgi | 1.2 | 0.2 | -4.6 | -4.2 | -3.1 | -4.7 | -3.3 | -4.0 | -1.8 | 4.5 | 6.7 | 7.9 | 2.1 | 4.2 | 2.1 |
Mitochondria | 5.3 | 1.5 | 5.2 | 7.8 | 4.1 | 6.9 | 5.4 | 6.0 | 5.7 | -0.1 | -2.8 | -2.8 | -15.8 | -12.0 | -13.9 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -1.7 | 1.1 | 2.3 | 0.8 | 1.8 | 1.3 | 1.2 | 2.4 | 1.1 | -1.1 | -1.6 | 0 | -1.8 | 0 | 0 |
Nucleolus | -1.2 | 1.2 | 2.4 | 2.0 | 2.5 | 2.5 | 3.1 | 4.1 | 1.9 | -0.2 | -1.1 | -0.6 | -1.0 | 0 | 0 |
Peroxisomes | -0.4 | -2.9 | -5.8 | -6.5 | -4.7 | -6.0 | -5.7 | -6.8 | -5.7 | 0.4 | 2.1 | 2.5 | 7.3 | 4.2 | 7.4 |
SpindlePole | -2.1 | -5.3 | -4.3 | -3.3 | -3.3 | -4.5 | -3.1 | -4.2 | -4.2 | -1.9 | -3.8 | -4.0 | 0.7 | 0.2 | 0.4 |
Vacuole | -1.8 | 0.6 | -2.9 | -2.5 | -3.1 | -3.4 | -3.6 | -4.1 | -4.0 | 0.6 | 0.6 | 0 | 1.1 | 0.9 | 0.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 30.1714 | 34.6875 | 32.0918 | 29.5816 | 27.8095 | 34.0157 | 49.4011 | 42.4697 | 44.6575 | 42.6657 | 43.9458 | 42.5558 | 39.3623 | 40.9821 | 40.8039 | 36.7091 | 34.7338 | 39.3281 |
Actin | 0.0046 | 0.0092 | 0.0053 | 0.0003 | 0.0025 | 0.0047 | 0.0007 | 0.0011 | 0.0003 | 0.0001 | 0.0005 | 0.0002 | 0.002 | 0.0007 | 0.002 | 0.0027 | 0.0019 | 0.0001 |
Bud | 0.0006 | 0.0012 | 0.0005 | 0 | 0.0002 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Bud Neck | 0.0001 | 0.0003 | 0.0014 | 0 | 0.0004 | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Bud Periphery | 0.0004 | 0.0017 | 0.0012 | 0 | 0.0003 | 0.0004 | 0 | 0 | 0 | 0.0002 | 0.0004 | 0 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 |
Bud Site | 0.0015 | 0.0025 | 0.0017 | 0.0002 | 0.0012 | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0005 | 0.0018 | 0.0003 | 0 | 0.0001 | 0 |
Cell Periphery | 0.0007 | 0.0039 | 0.0008 | 0.0003 | 0.0007 | 0.0009 | 0.0003 | 0.0004 | 0.0007 | 0.0002 | 0.0001 | 0.0001 | 0.0012 | 0.0006 | 0.002 | 0.0002 | 0.0003 | 0.0001 |
Cytoplasm | 0.0011 | 0.0001 | 0.0023 | 0.0021 | 0.0011 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0.001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0 |
Cytoplasmic Foci | 0.0151 | 0.0008 | 0.012 | 0.0196 | 0.0089 | 0.0004 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0033 | 0.0007 | 0.0034 | 0.0003 | 0.0035 | 0.0001 |
Eisosomes | 0.0007 | 0.0015 | 0.0022 | 0.0001 | 0.0003 | 0.0012 | 0.0007 | 0.0009 | 0.0014 | 0.0001 | 0.0017 | 0.0009 | 0.0017 | 0.0005 | 0.0003 | 0.0019 | 0.0004 | 0.0002 |
Endoplasmic Reticulum | 0.0005 | 0.0009 | 0.0002 | 0.0006 | 0.0009 | 0.0006 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0008 | 0 | 0.0002 | 0.0001 | 0.0002 | 0 |
Endosome | 0.0078 | 0.0025 | 0.0035 | 0.0018 | 0.0047 | 0.0037 | 0.0003 | 0.0003 | 0.0002 | 0 | 0.0002 | 0.0003 | 0.0025 | 0.0014 | 0.0019 | 0.002 | 0.0023 | 0.0002 |
Golgi | 0.0103 | 0.0075 | 0.0075 | 0.0009 | 0.0033 | 0.012 | 0.0024 | 0.0013 | 0.0006 | 0 | 0.0004 | 0.0041 | 0.0027 | 0.0053 | 0.0036 | 0.0042 | 0.0013 | 0.0016 |
Lipid Particles | 0.0137 | 0.0213 | 0.0089 | 0.0061 | 0.0228 | 0.0116 | 0.0039 | 0.0018 | 0.0058 | 0.0001 | 0.0003 | 0.0019 | 0.0152 | 0.0056 | 0.0053 | 0.0078 | 0.0164 | 0.0061 |
Mitochondria | 0.8876 | 0.8759 | 0.9039 | 0.918 | 0.9154 | 0.9277 | 0.9696 | 0.9713 | 0.9687 | 0.9979 | 0.9939 | 0.9836 | 0.9108 | 0.9484 | 0.9571 | 0.9544 | 0.9555 | 0.9751 |
None | 0.0018 | 0.0001 | 0.0028 | 0.0023 | 0.0006 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0011 | 0 | 0.0003 | 0 | 0.0009 | 0 |
Nuclear Periphery | 0.003 | 0.0005 | 0.0008 | 0.0025 | 0.0022 | 0.0015 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0031 | 0.0003 | 0.0008 | 0.0006 | 0.0025 | 0 |
Nucleolus | 0.0087 | 0.0063 | 0.0108 | 0.0009 | 0.005 | 0.0014 | 0.0026 | 0.0002 | 0.0002 | 0 | 0 | 0.0001 | 0.0108 | 0.0013 | 0.0032 | 0.0001 | 0.0073 | 0.0004 |
Nucleus | 0.002 | 0.0006 | 0.0021 | 0.0009 | 0.0017 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.004 | 0.0004 | 0.0008 | 0.0001 | 0.0005 | 0 |
Peroxisomes | 0.0059 | 0.0023 | 0.0057 | 0.0007 | 0.0053 | 0.0008 | 0.0004 | 0.0005 | 0.0004 | 0.0001 | 0.0003 | 0.0002 | 0.0024 | 0.0018 | 0.001 | 0.0006 | 0.0021 | 0.0002 |
Punctate Nuclear | 0.004 | 0 | 0.0013 | 0.0137 | 0.0092 | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0036 | 0.0034 | 0.0004 | 0 | 0.002 | 0 |
Vacuole | 0.0048 | 0.0131 | 0.0061 | 0.0019 | 0.004 | 0.0032 | 0.0021 | 0.0024 | 0.0033 | 0.0002 | 0.0003 | 0.0004 | 0.0045 | 0.0043 | 0.0048 | 0.002 | 0.0007 | 0.0006 |
Vacuole Periphery | 0.025 | 0.048 | 0.019 | 0.0271 | 0.0094 | 0.0276 | 0.0168 | 0.0197 | 0.0179 | 0.001 | 0.0015 | 0.0081 | 0.0281 | 0.0231 | 0.012 | 0.0226 | 0.0019 | 0.0153 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 159.958 | 202.1935 | 144.6296 | 192.5414 | 192.8618 | 295.3002 | 542.4042 | 461.1817 | 451.6179 | 366.8166 |
Translational Efficiency | 1.7501 | 1.7562 | 2.1538 | 1.9171 | 1.8417 | 2.0281 | 1.8195 | 2.1663 | 1.8746 | 1.8966 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✔ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1398 | 380 | 2682 | 1272 | 1980 | 1897 | 656 | 1222 | 3378 | 2277 | 3338 | 2494 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 867.40 | 1150.48 | 1030.05 | 1450.32 | 921.49 | 989.10 | 1655.16 | 1784.71 | 899.10 | 1016.03 | 1152.90 | 1614.16 |
Standard Deviation | 180.64 | 263.57 | 530.42 | 514.35 | 224.53 | 219.41 | 755.65 | 636.04 | 209.20 | 235.21 | 632.44 | 600.91 |
Intensity Change Log 2 | — | 0.407467 | 0.247945 | 0.741602 | — | 0.102148 | 0.844930 | 0.953649 | — | 0.258263 | 0.585970 | 0.854714 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.002986 | 0.005675 | 0.009454 | 0.011269 | 0.002146 | 0.005131 | 0.026801 | 0.007963 | 0.002494 | 0.005222 | 0.012863 | 0.009649 |
Bud Neck | 0.001448 | 0.002174 | 0.008539 | 0.002781 | 0.000526 | 0.001422 | 0.004855 | 0.004416 | 0.000907 | 0.001547 | 0.007815 | 0.003582 |
Bud Site | 0.003451 | 0.004625 | 0.037742 | 0.007773 | 0.002875 | 0.003531 | 0.004536 | 0.011621 | 0.003113 | 0.003713 | 0.031216 | 0.009659 |
Cell Periphery | 0.000376 | 0.000170 | 0.000907 | 0.000368 | 0.000278 | 0.000178 | 0.000356 | 0.000811 | 0.000319 | 0.000176 | 0.000799 | 0.000585 |
Cytoplasm | 0.000604 | 0.001156 | 0.008258 | 0.001225 | 0.000339 | 0.000846 | 0.000681 | 0.001958 | 0.000449 | 0.000898 | 0.006769 | 0.001584 |
Cytoplasmic Foci | 0.012878 | 0.045256 | 0.022396 | 0.014383 | 0.009845 | 0.020533 | 0.033998 | 0.011421 | 0.011100 | 0.024659 | 0.024676 | 0.012931 |
Eisosomes | 0.000079 | 0.000262 | 0.000238 | 0.000519 | 0.000121 | 0.000144 | 0.001555 | 0.000826 | 0.000103 | 0.000164 | 0.000497 | 0.000670 |
Endoplasmic Reticulum | 0.000178 | 0.000265 | 0.000461 | 0.000219 | 0.000120 | 0.000193 | 0.000914 | 0.000441 | 0.000144 | 0.000205 | 0.000550 | 0.000328 |
Endosome | 0.022204 | 0.024221 | 0.055469 | 0.009187 | 0.009376 | 0.020619 | 0.019873 | 0.009424 | 0.014685 | 0.021220 | 0.048473 | 0.009303 |
Golgi | 0.017429 | 0.067501 | 0.028431 | 0.023647 | 0.015817 | 0.035829 | 0.134308* | 0.023995 | 0.016484 | 0.041115 | 0.049238 | 0.023818 |
Lipid Particles | 0.007317 | 0.008757 | 0.003311 | 0.003331 | 0.003878 | 0.005633 | 0.031471 | 0.006599 | 0.005301 | 0.006154 | 0.008845 | 0.004932 |
Mitochondria | 0.893694* | 0.781419* | 0.727892* | 0.883656* | 0.934031* | 0.870125* | 0.685102* | 0.873949* | 0.917337* | 0.855321* | 0.719483* | 0.878900* |
Mitotic Spindle | 0.002478 | 0.003250 | 0.027930 | 0.006691 | 0.000870 | 0.001942 | 0.005233 | 0.003631 | 0.001535 | 0.002161 | 0.023470 | 0.005192 |
None | 0.000277 | 0.000577 | 0.000778 | 0.001205 | 0.000494 | 0.000389 | 0.002240 | 0.001972 | 0.000404 | 0.000421 | 0.001065 | 0.001581 |
Nuclear Periphery | 0.000073 | 0.000188 | 0.000585 | 0.000722 | 0.000073 | 0.000096 | 0.001933 | 0.000779 | 0.000073 | 0.000111 | 0.000850 | 0.000750 |
Nuclear Periphery Foci | 0.000179 | 0.000275 | 0.000544 | 0.000329 | 0.000108 | 0.000229 | 0.000773 | 0.000520 | 0.000137 | 0.000237 | 0.000589 | 0.000423 |
Nucleolus | 0.000078 | 0.000392 | 0.000816 | 0.000252 | 0.000335 | 0.000555 | 0.000945 | 0.001524 | 0.000229 | 0.000528 | 0.000842 | 0.000876 |
Nucleus | 0.000052 | 0.000087 | 0.001555 | 0.000069 | 0.000081 | 0.000362 | 0.000245 | 0.000548 | 0.000069 | 0.000316 | 0.001298 | 0.000304 |
Peroxisomes | 0.028478 | 0.046348 | 0.028604 | 0.015838 | 0.015567 | 0.026937 | 0.009095 | 0.010426 | 0.020910 | 0.030176 | 0.024770 | 0.013186 |
Vacuole | 0.002752 | 0.004652 | 0.022348 | 0.005875 | 0.001416 | 0.002448 | 0.012420 | 0.012735 | 0.001969 | 0.002816 | 0.020397 | 0.009236 |
Vacuole Periphery | 0.002989 | 0.002753 | 0.013741 | 0.010660 | 0.001705 | 0.002859 | 0.022666 | 0.014441 | 0.002236 | 0.002841 | 0.015495 | 0.012513 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.57 | -5.83 | -4.82 | -2.96 | -0.91 | -3.73 | -7.16 | -4.33 | -1.94 | 5.16 | -4.06 | -9.43 | -6.54 | -3.66 | 2.19 |
Bud Neck | -1.25 | -8.68 | -2.33 | -1.09 | 6.81 | -3.22 | -5.98 | -5.09 | -3.74 | 0.36 | -2.23 | -11.16 | -5.77 | -4.20 | 5.70 |
Bud Site | -0.56 | -15.16 | -2.87 | -1.42 | 12.15 | -0.59 | -1.20 | -4.03 | -3.82 | -3.10 | -0.67 | -15.44 | -4.97 | -4.43 | 10.30 |
Cell Periphery | 2.19 | -3.75 | 0.05 | -1.91 | 3.63 | 1.05 | -0.79 | -3.60 | -5.29 | -3.42 | 2.07 | -4.30 | -2.70 | -5.18 | 1.84 |
Cytoplasm | -1.93 | -9.89 | -2.17 | -0.20 | 8.68 | -2.67 | -2.37 | -2.93 | -1.95 | -2.17 | -2.73 | -10.13 | -3.69 | -2.03 | 7.60 |
Cytoplasmic Foci | -6.41 | -5.60 | -0.80 | 6.05 | 4.44 | -6.21 | -7.72 | -1.08 | 4.77 | 6.99 | -8.26 | -9.96 | -1.55 | 6.72 | 8.01 |
Eisosomes | -4.58 | -2.98 | -6.41 | -3.27 | -3.24 | -1.53 | -5.34 | -9.16 | -8.85 | 2.62 | -4.58 | -5.73 | -10.96 | -9.65 | -2.01 |
Endoplasmic Reticulum | -1.41 | -6.63 | -1.32 | 0.73 | 5.48 | -2.02 | -6.60 | -4.00 | -3.10 | 3.34 | -2.11 | -9.65 | -4.29 | -2.69 | 4.06 |
Endosome | -0.52 | -10.81 | 5.90 | 4.24 | 18.04 | -6.15 | -4.55 | -0.09 | 5.47 | 4.19 | -3.75 | -15.43 | 4.24 | 7.19 | 18.48 |
Golgi | -6.80 | -5.07 | -2.31 | 5.86 | 1.86 | -7.72 | -13.73 | -2.80 | 3.45 | 12.37 | -10.07 | -13.73 | -3.72 | 6.23 | 9.32 |
Lipid Particles | -0.63 | 2.76 | 2.67 | 2.83 | 0.01 | -1.57 | -6.43 | -1.79 | -0.61 | 5.58 | -0.78 | -3.07 | 0.38 | 1.09 | 3.26 |
Mitochondria | 6.47 | 18.64 | 1.22 | -5.82 | -17.15 | 8.98 | 17.36 | 7.12 | -0.29 | -12.02 | 9.40 | 28.81 | 6.48 | -3.06 | -20.80 |
Mitotic Spindle | -0.84 | -14.90 | -3.48 | -2.86 | 10.60 | -2.64 | -3.54 | -2.93 | -1.83 | 0.89 | -1.49 | -16.31 | -4.82 | -4.05 | 11.86 |
None | -4.58 | -7.47 | -11.67 | -7.31 | -4.59 | 1.27 | -9.39 | -10.60 | -12.28 | 1.27 | -0.28 | -9.30 | -14.34 | -14.65 | -5.57 |
Nuclear Periphery | -3.97 | -3.74 | -4.41 | -3.58 | -0.65 | -0.87 | -4.68 | -4.05 | -3.94 | 2.66 | -2.12 | -5.72 | -5.94 | -5.63 | 0.58 |
Nuclear Periphery Foci | -1.11 | -3.66 | -2.00 | -0.63 | 2.11 | -0.75 | -3.62 | -3.66 | -2.43 | 1.28 | -0.88 | -4.93 | -4.10 | -2.11 | 1.76 |
Nucleolus | -1.73 | -4.81 | -4.39 | 0.76 | 3.70 | -0.13 | -0.97 | -1.91 | -1.74 | -0.93 | -0.67 | -2.57 | -2.00 | -1.03 | -0.09 |
Nucleus | -1.10 | -4.72 | -1.04 | 0.58 | 4.68 | -1.67 | -1.29 | -2.71 | -0.82 | -1.39 | -1.80 | -4.75 | -2.73 | 0.08 | 3.79 |
Peroxisomes | -2.57 | -0.08 | 3.73 | 4.51 | 4.65 | -3.93 | 2.53 | 2.21 | 6.05 | -0.57 | -3.48 | -1.83 | 3.87 | 6.69 | 5.84 |
Vacuole | -1.06 | -15.03 | -3.71 | -0.69 | 12.35 | -3.09 | -8.32 | -7.74 | -6.97 | -0.18 | -1.91 | -18.08 | -8.83 | -7.44 | 8.94 |
Vacuole Periphery | 0.41 | -10.36 | -5.82 | -6.15 | 1.99 | -2.87 | -10.21 | -7.83 | -6.98 | 3.15 | -1.61 | -15.11 | -9.93 | -9.12 | 2.25 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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