Standard name
Human Ortholog
Description Protein of unknown function; potential Cdc28p substrate; overproduction confers resistance to methylmercury
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.3 0.26 0.31 0.32 0.39 0.4 0.22 0.23 0.18 0.13 0.12 0.08 0.42 0.39 0.42 0.08 0.12 0.08 0.19 0.29 0.11 0.15 0.31 0.29
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0 0 0 0 0 0
Endosome 0 0 0.09 0.05 0.12 0 0 0 0 0 0 0 0 0 0.1 0.06 0 0.07 0.06 0 0.07 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.68 0.29 0.15 0.4 0.27 0.52 0.67 0.56 0.65 0.7 0.78 0.72 0 0 0 0.06 0.09 0 0.18 0.06 0.16 0 0 0.05
Nucleus 0.13 0.53 0.35 0.16 0.18 0.14 0.24 0.26 0.35 0.38 0.39 0.43 0.45 0.49 0.36 0.73 0.72 0.7 0.15 0.3 0.22 0.35 0.22 0.22
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.08 0.12 0.05 0 0 0.08 0.05 0 0 0 0.05 0 0 0 0 0 0.07 0.08 0.12 0.19 0.13 0.13
Vac/Vac Membrane 0.13 0.21 0.14 0.1 0.1 0.09 0.07 0 0.06 0 0 0 0.09 0.08 0.14 0.12 0.07 0.13 0.21 0.15 0.18 0.12 0.14 0.15
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud 0 0 0 2 4 4 12 12 11 16 14 14 0 0 0 0 0 0 1 1 3 0 5 5
Bud Neck 0 0 0 0 2 3 0 2 1 0 1 0 2 1 1 0 0 0 0 0 1 0 3 4
Bud Site 0 0 0 2 1 4 12 4 7 19 15 12 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 25 9 52 76 155 145 137 141 82 69 48 33 63 87 97 18 21 12 18 24 28 6 52 57
Endoplasmic Reticulum 1 0 0 1 1 0 0 0 0 0 0 0 0 2 0 3 13 8 2 0 2 0 0 0
Endosome 0 0 16 13 47 13 3 27 2 4 1 6 1 1 24 14 4 10 6 2 19 1 5 6
Golgi 0 0 0 3 0 0 0 0 0 0 0 0 0 0 1 0 2 1 1 0 1 0 0 0
Mitochondria 56 10 25 96 108 188 405 343 295 379 302 307 3 2 1 15 15 6 17 5 41 1 5 9
Nucleus 11 18 59 39 71 50 147 162 156 205 152 182 68 109 83 173 126 101 14 25 56 13 36 43
Nuclear Periphery 0 0 0 0 0 1 1 1 1 1 3 5 0 0 0 3 0 0 0 0 2 0 0 1
Nucleolus 1 0 0 0 3 0 8 10 6 16 11 14 0 0 0 9 5 3 0 2 12 1 5 6
Peroxisomes 0 0 0 2 0 0 2 0 0 1 1 0 0 0 1 1 1 0 0 0 0 0 0 0
SpindlePole 2 0 14 29 21 15 27 51 22 18 13 11 7 8 7 6 4 2 6 6 31 7 22 25
Vac/Vac Membrane 11 7 23 23 40 34 40 26 25 20 16 16 13 17 32 29 12 19 20 12 46 4 22 29
Unique Cell Count 82 34 169 241 397 365 609 617 452 542 389 424 150 221 232 236 174 144 98 83 260 40 167 199
Labelled Cell Count 107 44 189 286 453 457 794 779 608 748 577 600 157 227 247 271 203 162 98 83 260 40 167 199


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 4.4 4.3 4.1 4.1 3.8 3.4 3.5 3.7 3.4 3.4 3.2 5.6 5.4 5.2 6.6 7.0 7.0 – 5.5 6.1
Std Deviation (1e-4) 0.9 0.7 1.1 1.9 1.3 2.1 1.6 1.5 2.0 1.5 1.8 0.7 1.3 1.1 1.1 1.4 1.7 1.6 – 1.4 1.7
Intensity Change (Log2) – – – -0.07 -0.07 -0.18 -0.31 -0.27 -0.22 -0.32 -0.32 -0.42 0.39 0.35 0.3 0.64 0.72 0.73 – 0.36 0.52


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – -0.4 -2.1 -0.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.7757 0.405 0.5017 -0.0086 0.7382 0.1917 -1.0447 -0.8101 -1.1803 -0.9365 -1.0814 -1.0533 -2.4244 -1.507 -1.8002 -1.7135 -1.6311 -1.7308
Actin 0.0355 0.0003 0.0086 0.002 0.0398 0.0027 0.0328 0.0007 0.0071 0.0043 0.0234 0.0099 0.0033 0.0006 0.0005 0.0007 0.0004 0.0003
Bud 0.0007 0.0003 0.0011 0.0001 0.001 0.0001 0.0013 0.0002 0.0002 0.0002 0.0007 0.0003 0.0008 0.0088 0.0001 0.0002 0.0002 0.0001
Bud Neck 0.0062 0.0007 0.0047 0.0006 0.002 0.0042 0.0143 0.0006 0.0007 0.0004 0.0018 0.0064 0.0016 0.0007 0.0004 0.0015 0.0027 0.0049
Bud Periphery 0.0019 0.0002 0.0014 0.0001 0.0022 0.0001 0.0019 0.0002 0.0002 0.0002 0.0011 0.0005 0.001 0.0014 0.0001 0.0002 0.0002 0.0002
Bud Site 0.0043 0.002 0.0116 0.0003 0.0026 0.0002 0.0053 0.0016 0.0006 0.0007 0.0026 0.0006 0.0016 0.0026 0.0003 0.0003 0.0005 0.0003
Cell Periphery 0.001 0 0.0003 0 0.0002 0 0.0004 0.0001 0.0001 0 0.0002 0.0001 0.0002 0.0001 0 0 0.0001 0.0001
Cytoplasm 0.0425 0.043 0.0375 0.0492 0.0346 0.0291 0.0397 0.0574 0.0403 0.0116 0.0901 0.03 0.0475 0.0429 0.0487 0.0909 0.0864 0.0191
Cytoplasmic Foci 0.0191 0.0028 0.0083 0.0128 0.0202 0.0025 0.0188 0.0102 0.0052 0.0143 0.0163 0.0072 0.0063 0.0042 0.0065 0.008 0.0113 0.0065
Eisosomes 0.0004 0.0001 0.0001 0.0001 0.0002 0.0001 0.0004 0 0.0001 0 0.0002 0.0001 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0088 0.0021 0.0017 0.0019 0.0034 0.0009 0.0075 0.0031 0.0021 0.0007 0.0047 0.0019 0.0039 0.0022 0.0031 0.0045 0.0054 0.001
Endosome 0.0364 0.0027 0.0118 0.025 0.033 0.0037 0.0353 0.0032 0.0126 0.0126 0.026 0.0122 0.0078 0.0037 0.0057 0.0053 0.0125 0.0034
Golgi 0.0085 0.0001 0.0068 0.0105 0.0181 0.0005 0.0093 0.0002 0.0029 0.0053 0.0116 0.007 0.0009 0.0004 0.0002 0.0003 0.0002 0.0002
Lipid Particles 0.0141 0.0005 0.0056 0.0108 0.0389 0.0012 0.0203 0.0006 0.01 0.026 0.0346 0.0039 0.0018 0.0039 0.0009 0.0004 0.0008 0.0022
Mitochondria 0.0121 0.0006 0.0062 0.0043 0.0082 0.0007 0.0144 0.0005 0.0043 0.0032 0.0086 0.0124 0.0009 0.0023 0.0004 0.0006 0.0011 0.0003
None 0.0898 0.1046 0.0558 0.1075 0.1245 0.0404 0.0498 0.056 0.0793 0.0509 0.0844 0.0308 0.0571 0.0202 0.0586 0.0601 0.1174 0.0412
Nuclear Periphery 0.0581 0.026 0.0249 0.0403 0.0477 0.0235 0.063 0.0431 0.0539 0.0212 0.0453 0.0299 0.0467 0.07 0.052 0.0352 0.0153 0.0173
Nucleolus 0.0231 0.025 0.0174 0.0075 0.0337 0.0493 0.0168 0.0231 0.0099 0.0086 0.0092 0.0444 0.023 0.0291 0.0111 0.0145 0.0153 0.0457
Nucleus 0.5105 0.7097 0.7146 0.6742 0.4702 0.7062 0.5773 0.7443 0.7227 0.7857 0.5669 0.7347 0.6776 0.7129 0.7428 0.5211 0.7056 0.7536
Peroxisomes 0.0073 0.0002 0.0022 0.0096 0.0281 0.0008 0.0094 0.0006 0.0031 0.0144 0.0032 0.0034 0.0006 0.0003 0.0002 0.0003 0.0001 0.0002
Punctate Nuclear 0.1107 0.0773 0.076 0.0409 0.0823 0.1329 0.0735 0.0522 0.0409 0.0389 0.0637 0.0605 0.1101 0.0917 0.0654 0.2538 0.0165 0.1021
Vacuole 0.0057 0.0014 0.0023 0.0013 0.0061 0.0006 0.0058 0.0019 0.0025 0.0004 0.0033 0.0028 0.0063 0.001 0.002 0.0013 0.0073 0.0013
Vacuole Periphery 0.0031 0.0004 0.0008 0.0009 0.0028 0.0003 0.0029 0.0005 0.0017 0.0005 0.0021 0.0011 0.0012 0.0008 0.0012 0.0006 0.0007 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.8013 6.6255 11.6765 11.9024 5.4053 5.5556 11.7234 12.4339 9.4406 11.3286
Translational Efficiency 0.2529 0.4703 0.3623 0.3352 0.3708 0.4229 0.2208 0.2486 0.402 0.2974

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
988 319 1744 184 725 285 748 381 1713 604 2492 565

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 630.97 711.73 793.91 754.48 653.36 717.04 789.43 804.29 640.45 714.24 792.57 788.07
Standard Deviation 67.82 77.67 95.14 130.67 81.18 77.26 100.37 91.77 74.59 77.52 96.76 108.56
Intensity Change Log 2 — 0.173759 0.331404 0.257911 — 0.134175 0.272933 0.299838 — 0.153758 0.301955 0.279392

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000091 0.000639 0.000800 0.001710 0.000209 0.000272 0.000711 0.000585 0.000141 0.000466 0.000773 0.000951
Bud Neck 0.005502 0.032784 0.010976 0.002323 0.002950 0.006333 0.009304 0.006040 0.004422 0.020303 0.010474 0.004830
Bud Site 0.000766 0.011728 0.004719 0.001426 0.001663 0.003900 0.002571 0.001325 0.001146 0.008034 0.004074 0.001358
Cell Periphery 0.000134 0.000185 0.000102 0.000202 0.000163 0.000183 0.000081 0.000117 0.000146 0.000184 0.000096 0.000144
Cytoplasm 0.320820* 0.226274* 0.037352 0.039239 0.392349* 0.198641* 0.040462 0.048422 0.351094* 0.213235* 0.038285 0.045431
Cytoplasmic Foci 0.127327 0.148741 0.001177 0.004913 0.055071 0.117348 0.001056 0.003729 0.096746 0.133928 0.001141 0.004115
Eisosomes 0.000099 0.000123 0.000031 0.000101 0.000051 0.000096 0.000033 0.000055 0.000079 0.000110 0.000032 0.000070
Endoplasmic Reticulum 0.001261 0.003243 0.006070 0.011428 0.002059 0.002087 0.006645 0.004085 0.001599 0.002698 0.006243 0.006476
Endosome 0.004134 0.013826 0.001485 0.001565 0.002407 0.007232 0.000984 0.001720 0.003403 0.010715 0.001335 0.001670
Golgi 0.000597 0.001387 0.000251 0.000246 0.000295 0.006661 0.000162 0.002832 0.000469 0.003875 0.000224 0.001990
Lipid Particles 0.018516 0.010873 0.000726 0.003044 0.004246 0.017776 0.000786 0.001375 0.012477 0.014130 0.000744 0.001918
Mitochondria 0.006922 0.009670 0.002725 0.000839 0.000896 0.023796 0.001867 0.001146 0.004372 0.016335 0.002468 0.001046
Mitotic Spindle 0.001982 0.010857 0.007059 0.000421 0.001991 0.012231 0.007436 0.002031 0.001986 0.011506 0.007172 0.001507
None 0.013964 0.009744 0.005483 0.015614 0.022625 0.008407 0.008993 0.012035 0.017630 0.009113 0.006536 0.013200
Nuclear Periphery 0.002027 0.001805 0.003137 0.001058 0.000810 0.002015 0.002966 0.001467 0.001512 0.001904 0.003085 0.001334
Nuclear Periphery Foci 0.003336 0.003485 0.001659 0.007885 0.003939 0.002007 0.002049 0.004479 0.003591 0.002788 0.001776 0.005588
Nucleolus 0.007722 0.010377 0.009670 0.020522 0.008008 0.012025 0.012186 0.020661 0.007843 0.011154 0.010426 0.020616
Nucleus 0.467474* 0.445682* 0.899051* 0.872693* 0.488259* 0.547452* 0.893215* 0.880371* 0.476271* 0.493703* 0.897299* 0.877871*
Peroxisomes 0.005976 0.020174 0.000523 0.000983 0.001052 0.008151 0.000353 0.000454 0.003892 0.014501 0.000472 0.000626
Vacuole 0.010886 0.036942 0.006345 0.013531 0.010647 0.022254 0.006310 0.006748 0.010785 0.030011 0.006335 0.008957
Vacuole Periphery 0.000464 0.001460 0.000658 0.000257 0.000309 0.001133 0.001830 0.000324 0.000398 0.001306 0.001009 0.000302

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.67 -12.01 -8.07 -5.05 -4.20 -0.77 -8.08 -6.93 -5.11 -0.48 -5.20 -13.80 -10.90 -6.28 -2.95
Bud Neck -4.62 -8.27 1.80 4.89 9.67 -2.12 -4.63 -3.86 -2.23 0.10 -4.87 -9.78 -3.07 3.15 3.18
Bud Site -4.64 -5.22 -3.72 3.67 2.21 -0.88 -1.97 -2.67 0.19 0.01 -4.23 -5.31 -5.06 2.97 1.94
Cell Periphery -3.20 3.91 -3.27 -0.86 -4.67 -0.21 6.89 2.96 1.60 -3.07 -1.87 7.39 -0.29 1.49 -4.91
Cytoplasm 5.80 33.50 24.48 11.90 -0.70 10.75 29.30 27.18 9.86 -1.08 11.46 44.58 37.02 15.48 -1.90
Cytoplasmic Foci -1.49 23.53 22.20 13.34 -2.66 -5.18 14.37 13.59 11.33 -3.31 -3.95 26.87 25.72 17.52 -4.23
Eisosomes -2.36 8.48 -1.29 0.73 -7.07 -4.79 3.42 -1.66 3.14 -4.28 -4.35 9.09 -0.34 3.70 -7.87
Endoplasmic Reticulum -4.86 -19.98 -9.67 -7.35 -4.24 -0.11 -8.38 -6.76 -6.63 2.07 -3.97 -19.47 -12.11 -9.06 -1.09
Endosome -4.47 4.33 3.38 5.57 -1.00 -3.62 5.82 2.11 4.45 -2.26 -5.51 5.88 3.87 6.93 -1.57
Golgi -2.26 3.46 3.27 3.29 -0.55 -2.64 3.66 -0.87 1.72 -1.03 -2.98 4.30 -0.70 1.69 -1.00
Lipid Particles 2.94 9.45 7.87 4.67 -3.77 -3.53 7.54 6.18 4.54 -2.45 -0.68 10.70 9.46 6.41 -4.70
Mitochondria -0.97 2.38 3.46 4.65 6.42 -3.49 -3.42 -2.82 3.41 1.66 -3.45 1.80 3.09 4.65 5.27
Mitotic Spindle -3.20 -5.25 0.24 3.34 5.79 -2.33 -3.75 -2.33 0.35 1.20 -3.90 -6.55 -2.24 1.61 2.13
None 1.95 5.07 0.24 -0.97 -2.34 5.65 6.44 4.63 -0.91 -1.40 5.09 8.61 3.00 -1.29 -3.45
Nuclear Periphery 0.29 -4.68 1.55 1.18 6.20 -4.29 -6.79 -6.37 -0.58 2.37 -2.28 -7.92 -2.87 -0.34 5.27
Nuclear Periphery Foci -0.72 -0.34 -1.84 -1.43 -1.77 2.29 2.88 0.72 -1.07 -1.38 0.91 1.49 -0.92 -1.54 -1.89
Nucleolus -0.46 -3.89 -2.66 -2.32 -0.85 -1.22 -1.74 -4.06 -2.67 -3.08 -1.29 -4.23 -4.95 -3.81 -2.92
Nucleus 1.06 -37.76 -22.23 -17.37 1.50 -2.63 -27.13 -22.37 -12.92 1.87 -1.17 -46.65 -32.46 -21.67 2.77
Peroxisomes -4.41 5.75 5.26 6.62 -2.40 -4.97 6.11 5.46 5.63 -1.45 -5.72 6.34 6.07 8.31 -1.56
Vacuole -6.06 1.46 -2.62 2.83 -3.10 -3.03 -0.47 -0.43 2.53 -0.01 -6.57 0.86 -1.95 4.49 -2.37
Vacuole Periphery -3.48 -0.15 2.31 4.09 1.07 -4.10 -1.16 0.29 3.85 1.19 -5.07 -1.16 1.39 5.40 1.54
Standard name
Human Ortholog
Description Protein of unknown function; potential Cdc28p substrate; overproduction confers resistance to methylmercury
Localization
Cell Percentages nucleus (39%), cytoplasm (13%), mixed (33%)
Cell Cycle Regulation Yes
cytoplasm - MA (3.5e-06)
Subcompartmental Group N/A

Bop3

Bop3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bop3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available