Standard name | |
---|---|
Human Ortholog | |
Description | Protein localized to COPII vesicles; proposed to be involved in ER to Golgi transport; interacts with members of the Rab GTPase family and Yip1p; also interacts with Rtn1p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 13 | 41 | 37 | 43 | 43 | 64 | 54 | 61 | 96 | 102 | 74 | 120 | 21 | 9 | 11 | 10 | 15 | 15 | 8 | 31 | 19 | 12 | 13 | 5 |
Bud | 3 | 3 | 4 | 1 | 4 | 8 | 13 | 12 | 13 | 14 | 15 | 10 | 2 | 1 | 0 | 2 | 3 | 3 | 1 | 3 | 0 | 3 | 1 | 9 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 3 | 4 | 3 | 2 | 8 | 2 | 6 | 10 | 9 | 10 | 1 | 3 | 3 | 1 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 30 | 35 | 25 | 59 | 58 | 46 | 56 | 41 | 47 | 58 | 54 | 93 | 46 | 49 | 61 | 35 | 23 | 19 | 12 | 16 | 6 | 15 | 15 | 31 |
Endoplasmic Reticulum | 71 | 83 | 67 | 76 | 139 | 106 | 129 | 87 | 76 | 105 | 83 | 109 | 80 | 100 | 103 | 5 | 5 | 4 | 56 | 63 | 44 | 57 | 71 | 74 |
Endosome | 0 | 0 | 1 | 1 | 1 | 0 | 2 | 3 | 2 | 8 | 6 | 6 | 0 | 1 | 0 | 1 | 2 | 1 | 5 | 10 | 6 | 5 | 3 | 4 |
Golgi | 58 | 98 | 105 | 78 | 110 | 104 | 149 | 111 | 152 | 236 | 108 | 155 | 44 | 66 | 52 | 87 | 96 | 81 | 83 | 110 | 119 | 61 | 39 | 45 |
Mitochondria | 0 | 0 | 8 | 4 | 17 | 76 | 85 | 69 | 95 | 77 | 103 | 153 | 0 | 0 | 0 | 19 | 19 | 21 | 2 | 3 | 1 | 1 | 4 | 4 |
Nucleus | 1 | 0 | 0 | 1 | 0 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 1 | 0 | 1 | 3 | 5 | 17 | 13 | 16 | 9 | 11 | 16 | 0 | 1 | 0 | 6 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 1 | 1 | 0 | 0 | 2 | 1 | 14 | 10 | 8 | 22 | 8 | 6 | 11 | 5 | 6 | 7 | 4 | 7 | 5 | 7 | 1 | 3 | 5 | 4 |
Unique Cell Count | 149 | 205 | 178 | 198 | 288 | 275 | 371 | 295 | 334 | 448 | 328 | 459 | 172 | 195 | 206 | 137 | 130 | 113 | 181 | 252 | 207 | 166 | 164 | 187 |
Labelled Cell Count | 178 | 263 | 250 | 268 | 380 | 414 | 532 | 411 | 512 | 643 | 472 | 679 | 205 | 235 | 236 | 174 | 169 | 158 | 181 | 252 | 207 | 166 | 164 | 187 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 30.2 | 36.8 | 25.3 | 22.5 | 25.1 | 22.0 | 21.6 | 21.7 | 22.5 | 22.2 | 21.3 | 22.1 | 30.8 | 30.2 | 29.1 | 34.1 | 39.8 | 38.9 | 33.8 | 34.4 | 39.9 |
Std Deviation (1e-4) | 6.1 | 5.9 | 4.4 | 5.0 | 4.8 | 5.3 | 5.0 | 5.8 | 5.4 | 5.3 | 5.9 | 5.8 | 5.1 | 5.3 | 5.4 | 14.2 | 14.1 | 14.7 | 7.3 | 10.9 | 12.5 |
Intensity Change (Log2) | – | – | – | -0.17 | -0.01 | -0.2 | -0.23 | -0.22 | -0.17 | -0.19 | -0.25 | -0.2 | 0.28 | 0.26 | 0.2 | 0.43 | 0.65 | 0.62 | 0.42 | 0.44 | 0.66 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.2 | -1.6 | 0.6 | -1.8 | 0 | 2.0 | 0.5 | 0.5 | 1.4 | -2.2 | -4.7 | -4.6 | -3.3 | -2.1 | -1.6 |
Bud | 0 | 0 | 0 | 0.8 | 1.1 | 1.0 | 0.6 | 1.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3.7 | 1.7 | 0.8 | 0.3 | 0 | 0 | -0.4 | 0.7 | 1.8 | 3.0 | 2.7 | 3.6 | 2.6 | 0.9 | 0.6 |
Endoplasmic Reticulum | 0.1 | 2.2 | 0.2 | -0.7 | -1.8 | -3.6 | -3.6 | -2.9 | -3.5 | 1.7 | 2.6 | 2.4 | -7.1 | -6.9 | -6.6 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | -3.8 | -4.4 | -4.4 | -4.1 | -4.5 | -2.9 | -1.4 | -5.7 | -5.8 | -6.3 | -4.9 | -6.7 | 0.8 | 2.7 | 2.2 |
Mitochondria | -1.4 | 0.7 | 6.2 | 5.4 | 5.4 | 6.4 | 4.2 | 7.0 | 7.5 | 0 | 0 | 0 | 2.9 | 3.1 | 3.9 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 2.9 | 0 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 | 0 | 0 | 3.4 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 21.4275 | 23.6301 | 23.4545 | 22.8066 | 21.3369 | 23.4067 | 19.3985 | 24.2837 | 23.3062 | 21.7216 | 22.1003 | 22.0208 | 21.8191 | 28.5529 | 24.8278 | 26.1396 | 22.6135 | 27.5803 |
Actin | 0.0242 | 0.0107 | 0.007 | 0.0101 | 0.0007 | 0.0119 | 0.0246 | 0.0139 | 0.0124 | 0.0023 | 0.0036 | 0.0035 | 0.0276 | 0.0127 | 0.0175 | 0.0085 | 0.0019 | 0.0094 |
Bud | 0.0002 | 0.0006 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0.0009 | 0.0007 | 0.0006 | 0.0001 | 0.0001 | 0.0007 | 0.0005 | 0.0003 | 0.0004 | 0 | 0.0001 |
Bud Neck | 0.0005 | 0.0004 | 0.0002 | 0.0003 | 0.0002 | 0.0004 | 0.0004 | 0.0005 | 0.0004 | 0.0015 | 0.0002 | 0.0003 | 0.0009 | 0.0011 | 0.0009 | 0.0004 | 0.0001 | 0.0006 |
Bud Periphery | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0004 | 0.0008 | 0.0017 | 0.0002 | 0.0001 | 0.0008 | 0.0005 | 0.0003 | 0.0006 | 0.0001 | 0.0001 |
Bud Site | 0.0003 | 0.0022 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.001 | 0.0012 | 0.001 | 0.0026 | 0.0001 | 0.0001 | 0.0028 | 0.0027 | 0.0006 | 0.0004 | 0 | 0.0001 |
Cell Periphery | 0.0004 | 0.0004 | 0.0003 | 0.0005 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0004 | 0.0007 | 0.0005 | 0.0001 | 0.0006 | 0.0021 | 0.0014 | 0.007 | 0.0009 | 0.0003 |
Cytoplasm | 0.0107 | 0.0378 | 0.0201 | 0.0223 | 0.0196 | 0.0154 | 0.0067 | 0.019 | 0.0118 | 0.0062 | 0.0051 | 0.0068 | 0.0151 | 0.0171 | 0.0165 | 0.0072 | 0.0025 | 0.0079 |
Cytoplasmic Foci | 0.0526 | 0.0912 | 0.0557 | 0.0467 | 0.036 | 0.0331 | 0.051 | 0.0741 | 0.0478 | 0.0399 | 0.0344 | 0.0353 | 0.0349 | 0.0505 | 0.0362 | 0.0252 | 0.0324 | 0.0296 |
Eisosomes | 0.0002 | 0 | 0 | 0 | 0 | 0.0001 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0.0002 | 0.0003 | 0 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0258 | 0.0171 | 0.0043 | 0.0642 | 0.0193 | 0.0212 | 0.0306 | 0.017 | 0.0194 | 0.0055 | 0.0444 | 0.0136 | 0.0214 | 0.0318 | 0.039 | 0.0776 | 0.0076 | 0.02 |
Endosome | 0.2448 | 0.2576 | 0.1986 | 0.2704 | 0.3291 | 0.166 | 0.2669 | 0.2804 | 0.2495 | 0.292 | 0.2732 | 0.173 | 0.1849 | 0.1708 | 0.1899 | 0.281 | 0.1946 | 0.144 |
Golgi | 0.6016 | 0.5367 | 0.6635 | 0.5241 | 0.5039 | 0.7266 | 0.525 | 0.5525 | 0.619 | 0.576 | 0.5364 | 0.7449 | 0.6448 | 0.6605 | 0.6411 | 0.4902 | 0.6473 | 0.7542 |
Lipid Particles | 0.0153 | 0.0316 | 0.0305 | 0.0285 | 0.0206 | 0.0125 | 0.0326 | 0.0308 | 0.0087 | 0.0291 | 0.0089 | 0.0101 | 0.0268 | 0.0299 | 0.0271 | 0.054 | 0.0945 | 0.0217 |
Mitochondria | 0.0123 | 0.0038 | 0.0101 | 0.01 | 0.0038 | 0.0032 | 0.0417 | 0.0025 | 0.0075 | 0.0089 | 0.0458 | 0.0046 | 0.0205 | 0.0098 | 0.0055 | 0.0072 | 0.0026 | 0.0039 |
None | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0008 | 0.0001 | 0.0011 | 0.0003 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0008 | 0.0001 |
Nuclear Periphery | 0.0036 | 0.0006 | 0.001 | 0.0022 | 0.0181 | 0.0036 | 0.004 | 0.0005 | 0.0033 | 0.0033 | 0.0042 | 0.002 | 0.0037 | 0.0006 | 0.003 | 0.0026 | 0.0024 | 0.0011 |
Nucleolus | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0043 | 0 | 0 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0 |
Nucleus | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0003 | 0.0003 | 0.0001 | 0.0008 | 0.0042 | 0.0002 | 0.0002 | 0.0003 | 0.0003 | 0.0007 | 0.0004 | 0.0001 | 0.0001 |
Peroxisomes | 0.0007 | 0.001 | 0.0004 | 0.0003 | 0.0002 | 0.0002 | 0.0033 | 0.0005 | 0.0031 | 0.0005 | 0.0002 | 0.0011 | 0.0012 | 0.0007 | 0.0007 | 0.0002 | 0.0031 | 0.0002 |
Punctate Nuclear | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0.0008 | 0.0001 | 0.0015 | 0.0014 | 0.0001 | 0.0004 | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0.0015 | 0.0001 |
Vacuole | 0.0029 | 0.005 | 0.0039 | 0.0098 | 0.0327 | 0.0018 | 0.0044 | 0.0028 | 0.0057 | 0.0122 | 0.0264 | 0.0016 | 0.0055 | 0.0057 | 0.0114 | 0.0262 | 0.0033 | 0.0025 |
Vacuole Periphery | 0.0033 | 0.0026 | 0.0032 | 0.0098 | 0.0144 | 0.0029 | 0.0048 | 0.0026 | 0.0049 | 0.0066 | 0.0158 | 0.0021 | 0.0063 | 0.0023 | 0.0072 | 0.0104 | 0.004 | 0.0038 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 125.7175 | 102.167 | 77.1907 | 75.2351 | 72.743 | 53.9974 | 111.7529 | 89.3699 | 65.8399 | 91.8051 |
Translational Efficiency | 1.3859 | 1.7804 | 1.6138 | 1.2391 | 1.739 | 3.2194 | 1.5756 | 1.5466 | 1.7775 | 1.646 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1569 | 1326 | 284 | 1164 | 1136 | 1599 | 1495 | 1609 | 2705 | 2925 | 1779 | 2773 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1891.56 | 1842.84 | 3736.93 | 3504.78 | 1612.91 | 1849.11 | 3417.43 | 3297.97 | 1774.54 | 1846.27 | 3468.44 | 3384.78 |
Standard Deviation | 410.89 | 420.49 | 542.81 | 679.97 | 360.11 | 382.81 | 514.76 | 799.17 | 413.89 | 400.34 | 532.36 | 758.34 |
Intensity Change Log 2 | — | -0.037646 | 0.982277 | 0.889747 | — | 0.197165 | 1.083246 | 1.031912 | — | 0.075187 | 1.029626 | 0.956922 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.002598 | 0.002184 | 0.013625 | 0.002324 | 0.001935 | 0.004950 | 0.001776 | 0.003076 | 0.002320 | 0.003696 | 0.003667 | 0.002760 |
Bud Neck | 0.001137 | 0.000836 | 0.019905 | 0.002676 | 0.000664 | 0.000963 | 0.002092 | 0.002010 | 0.000938 | 0.000906 | 0.004935 | 0.002290 |
Bud Site | 0.009610 | 0.003228 | 0.005972 | 0.014161 | 0.003910 | 0.005088 | 0.005753 | 0.018439 | 0.007216 | 0.004245 | 0.005788 | 0.016643 |
Cell Periphery | 0.005766 | 0.001152 | 0.008125 | 0.054775 | 0.001875 | 0.002287 | 0.004687 | 0.031247 | 0.004132 | 0.001773 | 0.005235 | 0.041123 |
Cytoplasm | 0.005184 | 0.001038 | 0.000137 | 0.014031 | 0.001315 | 0.001797 | 0.002148 | 0.016119 | 0.003559 | 0.001453 | 0.001827 | 0.015243 |
Cytoplasmic Foci | 0.065066 | 0.053369 | 0.008224 | 0.032833 | 0.060526 | 0.072413 | 0.046137 | 0.071136 | 0.063159 | 0.063780 | 0.040085 | 0.055058 |
Eisosomes | 0.000130 | 0.000086 | 0.000851 | 0.000286 | 0.000124 | 0.000353 | 0.000168 | 0.000326 | 0.000127 | 0.000232 | 0.000277 | 0.000309 |
Endoplasmic Reticulum | 0.279173* | 0.047418 | 0.043856 | 0.105758* | 0.118328* | 0.132903* | 0.082687 | 0.088430 | 0.211624* | 0.094150 | 0.076488 | 0.095704 |
Endosome | 0.062873 | 0.081040 | 0.033488 | 0.025485 | 0.078968 | 0.102218 | 0.068994 | 0.042997 | 0.069632 | 0.092617 | 0.063326 | 0.035646 |
Golgi | 0.430404* | 0.650711* | 0.444811* | 0.198888* | 0.603930* | 0.543960* | 0.463074* | 0.222878* | 0.503279* | 0.592354* | 0.460158* | 0.212808* |
Lipid Particles | 0.060965 | 0.107812* | 0.241209* | 0.435230* | 0.074344 | 0.100119* | 0.240315* | 0.411041* | 0.066584 | 0.103606* | 0.240457* | 0.421194* |
Mitochondria | 0.001058 | 0.000650 | 0.000749 | 0.001287 | 0.000324 | 0.001395 | 0.000580 | 0.002351 | 0.000750 | 0.001058 | 0.000607 | 0.001904 |
Mitotic Spindle | 0.000850 | 0.000304 | 0.000955 | 0.001703 | 0.000321 | 0.000412 | 0.001641 | 0.000952 | 0.000628 | 0.000363 | 0.001532 | 0.001267 |
None | 0.000662 | 0.000384 | 0.000137 | 0.000651 | 0.000205 | 0.000304 | 0.000229 | 0.000658 | 0.000470 | 0.000341 | 0.000214 | 0.000655 |
Nuclear Periphery | 0.000608 | 0.000282 | 0.004986 | 0.001285 | 0.000304 | 0.000186 | 0.000738 | 0.000555 | 0.000481 | 0.000230 | 0.001416 | 0.000862 |
Nuclear Periphery Foci | 0.011438 | 0.005611 | 0.013687 | 0.009154 | 0.008864 | 0.003683 | 0.008094 | 0.005895 | 0.010357 | 0.004557 | 0.008987 | 0.007263 |
Nucleolus | 0.000033 | 0.000495 | 0.000077 | 0.000026 | 0.000048 | 0.000056 | 0.000009 | 0.000092 | 0.000039 | 0.000255 | 0.000020 | 0.000065 |
Nucleus | 0.000263 | 0.000722 | 0.000111 | 0.000301 | 0.000133 | 0.000349 | 0.000065 | 0.000380 | 0.000208 | 0.000518 | 0.000072 | 0.000347 |
Peroxisomes | 0.000087 | 0.000102 | 0.000082 | 0.000668 | 0.000104 | 0.000209 | 0.000149 | 0.003183 | 0.000094 | 0.000161 | 0.000139 | 0.002127 |
Vacuole | 0.028743 | 0.027175 | 0.125599 | 0.081736 | 0.024468 | 0.015338 | 0.056383 | 0.060950 | 0.026948 | 0.020704 | 0.067432 | 0.069675 |
Vacuole Periphery | 0.033350 | 0.015399 | 0.033415 | 0.016741 | 0.019311 | 0.011015 | 0.014282 | 0.017284 | 0.027454 | 0.013003 | 0.017337 | 0.017056 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.58 | -2.55 | 0.22 | -0.29 | 2.59 | -3.35 | 0.20 | -1.82 | 2.02 | -2.43 | -2.29 | -1.64 | -0.95 | 1.44 | 1.02 |
Bud Neck | 1.50 | -6.27 | -5.65 | -6.29 | 5.69 | -1.98 | -5.62 | -6.84 | -4.64 | 0.29 | 0.15 | -7.34 | -8.18 | -7.73 | 4.62 |
Bud Site | 5.62 | 2.57 | -3.26 | -7.54 | -5.17 | -1.68 | -2.31 | -9.38 | -8.43 | -7.45 | 4.09 | 1.31 | -8.30 | -11.28 | -8.84 |
Cell Periphery | 13.26 | -2.07 | -15.69 | -17.27 | -14.07 | -1.39 | -8.37 | -15.16 | -14.82 | -13.54 | 9.16 | -2.98 | -21.20 | -22.62 | -20.33 |
Cytoplasm | 4.46 | 5.55 | -4.75 | -7.72 | -8.29 | -1.09 | -1.54 | -10.16 | -9.60 | -9.05 | 3.60 | 2.39 | -9.66 | -12.36 | -11.33 |
Cytoplasmic Foci | 3.35 | 19.13 | 8.91 | 6.24 | -9.04 | -3.29 | 4.17 | -2.44 | 0.39 | -6.11 | -0.25 | 8.37 | 2.83 | 3.18 | -5.01 |
Eisosomes | 2.68 | -4.62 | -5.27 | -7.62 | 3.60 | -2.02 | -2.10 | -8.51 | 0.24 | -6.13 | -1.67 | -4.88 | -9.70 | -1.20 | -0.93 |
Endoplasmic Reticulum | 29.43* | 25.31 | 19.82 | -10.03 | -8.09 | -1.86 | 5.04 | 4.39 | 7.20 | -1.02 | 19.40 | 21.97 | 19.71 | -0.23 | -4.17 |
Endosome | -6.33 | 10.58 | 20.50 | 21.67 | 3.22 | -7.12 | 3.26 | 13.26 | 23.78 | 11.77 | -10.78 | 2.97 | 21.24 | 31.37 | 15.19 |
Golgi | -21.19* | -0.54 | 23.01 | 45.90* | 12.23 | 5.60 | 12.85 | 37.67* | 35.22* | 24.90* | -11.53 | 5.01 | 39.81 | 56.12* | 29.60* |
Lipid Particles | -8.11 | -12.35 | -39.89* | -31.64* | -11.35 | -4.69 | -21.72 | -39.45* | -36.39* | -16.82 | -9.54 | -27.16 | -56.91 | -48.06* | -21.44 |
Mitochondria | 1.75 | 1.08 | -0.37 | -1.02 | -0.83 | -1.95 | -1.19 | -7.14 | -1.58 | -5.09 | -0.94 | 0.71 | -3.63 | -1.99 | -3.72 |
Mitotic Spindle | 5.90 | -0.69 | -2.80 | -4.46 | -2.07 | -0.74 | -3.20 | -4.66 | -3.27 | 1.70 | 3.02 | -2.69 | -4.26 | -5.59 | 0.86 |
None | 1.63 | 4.86 | 0.01 | -1.14 | -2.62 | -1.00 | -0.33 | -3.74 | -2.34 | -3.00 | 1.21 | 2.64 | -1.55 | -2.39 | -3.47 |
Nuclear Periphery | 6.51 | -4.42 | -7.71 | -11.81 | 3.75 | 2.98 | -6.28 | -4.62 | -8.18 | 2.59 | 7.83 | -5.47 | -7.69 | -14.05 | 3.28 |
Nuclear Periphery Foci | 6.52 | -0.94 | 2.60 | -4.91 | 1.72 | 3.66 | 0.36 | 1.96 | -4.31 | 3.16 | 7.54 | 1.17 | 3.85 | -6.28 | 2.49 |
Nucleolus | -1.57 | -1.86 | 0.47 | 1.60 | 2.58 | -0.32 | 1.86 | -1.12 | -0.70 | -2.34 | -1.62 | 1.50 | -1.09 | 1.41 | -2.12 |
Nucleus | -1.05 | 2.10 | -0.78 | 0.92 | -5.19 | -0.96 | 1.19 | -2.43 | -0.15 | -3.44 | -1.33 | 2.86 | -2.16 | 0.67 | -5.02 |
Peroxisomes | -0.51 | 0.17 | -5.12 | -4.88 | -5.10 | -2.18 | -1.17 | -8.88 | -8.51 | -8.71 | -2.23 | -1.38 | -9.77 | -9.37 | -9.47 |
Vacuole | 0.73 | -9.85 | -14.23 | -14.53 | 4.21 | 4.98 | -11.38 | -12.12 | -16.09 | -1.32 | 4.38 | -14.38 | -18.14 | -21.33 | -0.73 |
Vacuole Periphery | 10.21 | -0.01 | 9.53 | -1.00 | 5.25 | 5.60 | 3.60 | 1.26 | -5.48 | -2.93 | 12.25 | 8.25 | 8.60 | -4.64 | 0.28 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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