Standard name
Human Ortholog
Description Protein localized to COPII vesicles; proposed to be involved in ER to Golgi transport; interacts with members of the Rab GTPase family and Yip1p; also interacts with Rtn1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.09 0.2 0.21 0.22 0.15 0.23 0.15 0.21 0.29 0.23 0.23 0.26 0.12 0.05 0.05 0.07 0.12 0.13 0.05 0.12 0.1 0.08 0.08 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 0.17 0.14 0.3 0.2 0.17 0.15 0.14 0.14 0.13 0.16 0.2 0.27 0.25 0.3 0.26 0.18 0.17 0.07 0.06 0 0.09 0.1 0.17
Endoplasmic Reticulum 0.48 0.4 0.38 0.38 0.48 0.39 0.35 0.29 0.23 0.23 0.25 0.24 0.47 0.51 0.5 0 0 0 0.31 0.25 0.21 0.35 0.43 0.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.39 0.48 0.59 0.39 0.38 0.38 0.4 0.38 0.46 0.53 0.33 0.34 0.26 0.34 0.25 0.64 0.74 0.72 0.46 0.44 0.58 0.37 0.24 0.25
Mitochondria 0 0 0 0 0.06 0.28 0.23 0.23 0.28 0.17 0.31 0.33 0 0 0 0.14 0.15 0.19 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0.05 0 0 0.06 0 0 0.05 0 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 13 41 37 43 43 64 54 61 96 102 74 120 21 9 11 10 15 15 8 31 19 12 13 5
Bud 3 3 4 1 4 8 13 12 13 14 15 10 2 1 0 2 3 3 1 3 0 3 1 9
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud Site 0 0 0 0 0 1 0 0 1 1 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 3 4 3 2 8 2 6 10 9 10 1 3 3 1 0 2 0 0 1 0 0 0
Cytoplasm 30 35 25 59 58 46 56 41 47 58 54 93 46 49 61 35 23 19 12 16 6 15 15 31
Endoplasmic Reticulum 71 83 67 76 139 106 129 87 76 105 83 109 80 100 103 5 5 4 56 63 44 57 71 74
Endosome 0 0 1 1 1 0 2 3 2 8 6 6 0 1 0 1 2 1 5 10 6 5 3 4
Golgi 58 98 105 78 110 104 149 111 152 236 108 155 44 66 52 87 96 81 83 110 119 61 39 45
Mitochondria 0 0 8 4 17 76 85 69 95 77 103 153 0 0 0 19 19 21 2 3 1 1 4 4
Nucleus 1 0 0 1 0 1 2 2 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 1 0
Nucleolus 0 1 0 1 3 5 17 13 16 9 11 16 0 1 0 6 1 4 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 1
Vac/Vac Membrane 1 1 0 0 2 1 14 10 8 22 8 6 11 5 6 7 4 7 5 7 1 3 5 4
Unique Cell Count 149 205 178 198 288 275 371 295 334 448 328 459 172 195 206 137 130 113 181 252 207 166 164 187
Labelled Cell Count 178 263 250 268 380 414 532 411 512 643 472 679 205 235 236 174 169 158 181 252 207 166 164 187


Early Golgi, Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 30.2 36.8 25.3 22.5 25.1 22.0 21.6 21.7 22.5 22.2 21.3 22.1 30.8 30.2 29.1 34.1 39.8 38.9 33.8 34.4 39.9
Std Deviation (1e-4) 6.1 5.9 4.4 5.0 4.8 5.3 5.0 5.8 5.4 5.3 5.9 5.8 5.1 5.3 5.4 14.2 14.1 14.7 7.3 10.9 12.5
Intensity Change (Log2) – – – -0.17 -0.01 -0.2 -0.23 -0.22 -0.17 -0.19 -0.25 -0.2 0.28 0.26 0.2 0.43 0.65 0.62 0.42 0.44 0.66


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.2 -1.6 0.6 -1.8 0 2.0 0.5 0.5 1.4 -2.2 -4.7 -4.6 -3.3 -2.1 -1.6
Bud 0 0 0 0.8 1.1 1.0 0.6 1.3 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3.7 1.7 0.8 0.3 0 0 -0.4 0.7 1.8 3.0 2.7 3.6 2.6 0.9 0.6
Endoplasmic Reticulum 0.1 2.2 0.2 -0.7 -1.8 -3.6 -3.6 -2.9 -3.5 1.7 2.6 2.4 -7.1 -6.9 -6.6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi -3.8 -4.4 -4.4 -4.1 -4.5 -2.9 -1.4 -5.7 -5.8 -6.3 -4.9 -6.7 0.8 2.7 2.2
Mitochondria -1.4 0.7 6.2 5.4 5.4 6.4 4.2 7.0 7.5 0 0 0 2.9 3.1 3.9
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 2.9 0 3.0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 3.0 0 0 3.4 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 21.4275 23.6301 23.4545 22.8066 21.3369 23.4067 19.3985 24.2837 23.3062 21.7216 22.1003 22.0208 21.8191 28.5529 24.8278 26.1396 22.6135 27.5803
Actin 0.0242 0.0107 0.007 0.0101 0.0007 0.0119 0.0246 0.0139 0.0124 0.0023 0.0036 0.0035 0.0276 0.0127 0.0175 0.0085 0.0019 0.0094
Bud 0.0002 0.0006 0.0001 0.0002 0.0003 0.0001 0.0003 0.0009 0.0007 0.0006 0.0001 0.0001 0.0007 0.0005 0.0003 0.0004 0 0.0001
Bud Neck 0.0005 0.0004 0.0002 0.0003 0.0002 0.0004 0.0004 0.0005 0.0004 0.0015 0.0002 0.0003 0.0009 0.0011 0.0009 0.0004 0.0001 0.0006
Bud Periphery 0.0002 0.0002 0.0001 0.0002 0.0001 0.0001 0.0003 0.0004 0.0008 0.0017 0.0002 0.0001 0.0008 0.0005 0.0003 0.0006 0.0001 0.0001
Bud Site 0.0003 0.0022 0.0002 0.0001 0.0001 0.0001 0.001 0.0012 0.001 0.0026 0.0001 0.0001 0.0028 0.0027 0.0006 0.0004 0 0.0001
Cell Periphery 0.0004 0.0004 0.0003 0.0005 0.0002 0.0002 0.0002 0.0002 0.0004 0.0007 0.0005 0.0001 0.0006 0.0021 0.0014 0.007 0.0009 0.0003
Cytoplasm 0.0107 0.0378 0.0201 0.0223 0.0196 0.0154 0.0067 0.019 0.0118 0.0062 0.0051 0.0068 0.0151 0.0171 0.0165 0.0072 0.0025 0.0079
Cytoplasmic Foci 0.0526 0.0912 0.0557 0.0467 0.036 0.0331 0.051 0.0741 0.0478 0.0399 0.0344 0.0353 0.0349 0.0505 0.0362 0.0252 0.0324 0.0296
Eisosomes 0.0002 0 0 0 0 0.0001 0.0003 0 0.0001 0 0.0001 0 0.0002 0.0002 0.0003 0 0.0001 0.0001
Endoplasmic Reticulum 0.0258 0.0171 0.0043 0.0642 0.0193 0.0212 0.0306 0.017 0.0194 0.0055 0.0444 0.0136 0.0214 0.0318 0.039 0.0776 0.0076 0.02
Endosome 0.2448 0.2576 0.1986 0.2704 0.3291 0.166 0.2669 0.2804 0.2495 0.292 0.2732 0.173 0.1849 0.1708 0.1899 0.281 0.1946 0.144
Golgi 0.6016 0.5367 0.6635 0.5241 0.5039 0.7266 0.525 0.5525 0.619 0.576 0.5364 0.7449 0.6448 0.6605 0.6411 0.4902 0.6473 0.7542
Lipid Particles 0.0153 0.0316 0.0305 0.0285 0.0206 0.0125 0.0326 0.0308 0.0087 0.0291 0.0089 0.0101 0.0268 0.0299 0.0271 0.054 0.0945 0.0217
Mitochondria 0.0123 0.0038 0.0101 0.01 0.0038 0.0032 0.0417 0.0025 0.0075 0.0089 0.0458 0.0046 0.0205 0.0098 0.0055 0.0072 0.0026 0.0039
None 0.0001 0.0001 0.0003 0.0001 0.0001 0.0001 0.0008 0.0001 0.0011 0.0003 0.0002 0.0002 0.0003 0.0001 0.0001 0.0001 0.0008 0.0001
Nuclear Periphery 0.0036 0.0006 0.001 0.0022 0.0181 0.0036 0.004 0.0005 0.0033 0.0033 0.0042 0.002 0.0037 0.0006 0.003 0.0026 0.0024 0.0011
Nucleolus 0 0 0.0001 0 0 0 0.0001 0 0.0001 0.0043 0 0 0.0002 0.0002 0.0001 0.0002 0.0001 0
Nucleus 0.0002 0.0001 0.0002 0.0001 0.0003 0.0003 0.0003 0.0001 0.0008 0.0042 0.0002 0.0002 0.0003 0.0003 0.0007 0.0004 0.0001 0.0001
Peroxisomes 0.0007 0.001 0.0004 0.0003 0.0002 0.0002 0.0033 0.0005 0.0031 0.0005 0.0002 0.0011 0.0012 0.0007 0.0007 0.0002 0.0031 0.0002
Punctate Nuclear 0.0002 0.0002 0.0002 0.0001 0.0003 0.0002 0.0008 0.0001 0.0015 0.0014 0.0001 0.0004 0.0004 0.0002 0.0002 0.0002 0.0015 0.0001
Vacuole 0.0029 0.005 0.0039 0.0098 0.0327 0.0018 0.0044 0.0028 0.0057 0.0122 0.0264 0.0016 0.0055 0.0057 0.0114 0.0262 0.0033 0.0025
Vacuole Periphery 0.0033 0.0026 0.0032 0.0098 0.0144 0.0029 0.0048 0.0026 0.0049 0.0066 0.0158 0.0021 0.0063 0.0023 0.0072 0.0104 0.004 0.0038

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 125.7175 102.167 77.1907 75.2351 72.743 53.9974 111.7529 89.3699 65.8399 91.8051
Translational Efficiency 1.3859 1.7804 1.6138 1.2391 1.739 3.2194 1.5756 1.5466 1.7775 1.646

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein localized to COPII vesicles; proposed to be involved in ER to Golgi transport; interacts with members of the Rab GTPase family and Yip1p; also interacts with Rtn1p
Localization
Cell Percentages ER (22%), cytoplasm (1%), mixed (46%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Yip3

Yip3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yip3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available