ORF
Human Ortholog
Description Putative protein of unknown function; expression depends on Swi5p; GFP-fusion protein localizes to the endoplasmic reticulum; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.95 0.96 0.99 0.97 0.97 0.88 0.91 0.89 0.73 0.74 0.65 0.73 0.99 0.99 1.0 0.9 0.9 0.91 0.51 0.68 0.58 0.58 0.69 0.71
Endoplasmic Reticulum 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0.06 0.07 0.05 0.2 0.06 0.06 0.14 0.09 0.06
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.1 0.06 0.1 0.26 0.25 0.35 0.31 0 0 0 0.06 0 0 0.15 0.13 0.23 0.16 0.1 0.08
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 0 2 0 1 0 0 3 4 8 6 4 0 14 24 21
Bud 0 1 0 0 0 0 1 5 3 3 11 14 3 1 1 1 0 0 2 0 0 7 18 23
Bud Neck 0 0 0 3 6 3 6 4 3 3 3 7 1 0 1 2 5 2 2 2 0 9 12 16
Bud Site 0 0 0 0 0 0 0 0 0 0 3 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 2 2 5 1 2 1 2 3 2 2 3 2 3 2 6 5 7 4 0 2 20 22 31
Cytoplasm 312 172 85 236 353 155 278 513 176 223 186 273 811 1237 1389 517 305 382 186 121 60 520 741 823
Endoplasmic Reticulum 3 0 0 0 2 11 1 0 9 2 4 1 3 1 0 34 23 22 73 10 6 128 99 75
Endosome 4 1 0 0 0 0 4 3 0 1 4 0 2 2 1 11 2 2 7 1 2 5 5 8
Golgi 0 1 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 1 1 3 0 2 2 2
Mitochondria 7 5 1 4 5 17 18 58 62 76 101 117 3 3 0 35 7 9 56 22 23 148 102 96
Nucleus 0 0 0 0 2 1 1 3 5 2 2 2 0 0 0 1 2 1 0 0 0 0 1 0
Nuclear Periphery 1 0 0 0 0 0 1 0 0 1 0 1 0 2 1 0 0 0 0 0 0 1 1 0
Nucleolus 0 0 0 0 0 0 0 1 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 1 0 1 1 0 0 4 2 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 3 0 2 11 1 10 8 17 1 0 1 14 8 9 2 0 0 4 10 16
Vac/Vac Membrane 7 2 0 1 6 0 18 18 7 6 4 2 1 1 2 14 15 17 12 7 1 20 17 20
Unique Cell Count 327 180 86 243 364 176 305 579 241 300 287 373 823 1244 1395 572 340 418 365 179 105 902 1076 1157
Labelled Cell Count 338 184 88 250 378 189 331 619 269 330 331 440 829 1251 1398 642 378 460 365 179 105 902 1076 1157


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 4.5 5.6 4.9 5.7 3.6 4.6 4.6 3.8 4.0 3.8 3.7 4.5 4.5 4.7 5.4 5.8 5.9 4.1 4.1 4.2
Std Deviation (1e-4) 0.6 1.0 1.5 1.7 2.1 0.9 1.2 1.2 0.6 1.0 0.7 0.7 1.0 0.9 1.0 1.0 1.0 1.3 0.8 1.0 1.1
Intensity Change (Log2) – – – -0.19 0.01 -0.64 -0.3 -0.29 -0.58 -0.48 -0.56 -0.59 -0.31 -0.32 -0.27 -0.05 0.05 0.08 -0.45 -0.44 -0.41


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.8524 0.0467 -0.0521 -0.0463 0.284 -0.0435 0.5525 0.5253 0.53 0.7431 0.2534 0.6648 2.9054 2.6019 2.6095 2.4679 2.0019 2.726
Actin 0.0205 0.0008 0.0101 0.0038 0.051 0.0103 0.0135 0.0005 0.0115 0.0175 0.0089 0.0022 0.0127 0.0011 0.0314 0.0033 0.0047 0.0047
Bud 0.001 0.0014 0.0022 0.0004 0.0014 0.0003 0.0003 0.0017 0.0035 0.0002 0.0003 0.0001 0.0015 0.036 0.0127 0.0579 0.0003 0.0093
Bud Neck 0.0163 0.0003 0.0019 0.0009 0.0027 0.0069 0.0007 0.0002 0.0043 0.0042 0.0004 0.0007 0.0003 0.0007 0.0002 0.0004 0.0001 0.0003
Bud Periphery 0.0022 0.0019 0.002 0.0007 0.0027 0.0008 0.0004 0.0007 0.0026 0.0003 0.0004 0.0001 0.0012 0.0088 0.0062 0.0119 0.0012 0.0038
Bud Site 0.0095 0.0102 0.0072 0.0003 0.0114 0.0005 0.0024 0.0195 0.0125 0.0025 0.0012 0.0007 0.0015 0.0478 0.0066 0.0058 0.0001 0.0003
Cell Periphery 0.0008 0.0005 0.0003 0.0001 0.0003 0.0002 0.0003 0.0006 0.0005 0.0002 0.0002 0.0001 0.0004 0.0018 0.0008 0.0005 0.0001 0.0001
Cytoplasm 0.139 0.1174 0.1287 0.1123 0.2498 0.2167 0.2682 0.3335 0.303 0.3777 0.3403 0.3651 0.1021 0.1606 0.2269 0.0951 0.2964 0.3032
Cytoplasmic Foci 0.0308 0.0073 0.0141 0.0064 0.0115 0.0198 0.0298 0.0187 0.0179 0.0166 0.0094 0.0199 0.0423 0.0162 0.0155 0.0145 0.0196 0.0273
Eisosomes 0.0006 0.0002 0.0003 0.0002 0.0003 0.0002 0.0005 0.0002 0.0002 0.0003 0.0002 0.0001 0.0005 0.0005 0.0006 0.0003 0.0002 0.0001
Endoplasmic Reticulum 0.0193 0.0045 0.0084 0.0051 0.022 0.0093 0.051 0.0411 0.0169 0.0248 0.0059 0.0466 0.0354 0.0024 0.0246 0.0048 0.0222 0.0277
Endosome 0.0177 0.0022 0.0081 0.0134 0.0211 0.007 0.0307 0.0122 0.0253 0.0468 0.0084 0.0143 0.0338 0.0023 0.0113 0.0254 0.0018 0.0113
Golgi 0.0053 0.001 0.0013 0.0009 0.0071 0.0012 0.0093 0.002 0.0044 0.0115 0.0079 0.0114 0.0069 0.0002 0.0094 0.0053 0.0007 0.0069
Lipid Particles 0.0141 0.0016 0.0024 0.0018 0.0203 0.0034 0.0302 0.0034 0.0084 0.023 0.0038 0.0047 0.0361 0.0008 0.0041 0.0055 0.0024 0.0042
Mitochondria 0.0096 0.001 0.0013 0.0007 0.0055 0.0012 0.0056 0.0022 0.0059 0.0115 0.0277 0.0154 0.0041 0.0023 0.0113 0.0127 0.0011 0.0162
None 0.6217 0.8331 0.77 0.8002 0.5188 0.6956 0.4992 0.5505 0.5619 0.433 0.5768 0.5036 0.6846 0.6826 0.6193 0.7081 0.6119 0.5653
Nuclear Periphery 0.0282 0.0017 0.0097 0.0144 0.0348 0.0077 0.0071 0.0039 0.003 0.0033 0.0009 0.0037 0.0076 0.0011 0.0027 0.0038 0.0012 0.002
Nucleolus 0.003 0.0009 0.0015 0.0019 0.0054 0.0004 0.0007 0.0004 0.0005 0.0002 0.0002 0.0001 0.0015 0.0049 0.0009 0.0044 0.0001 0.0002
Nucleus 0.0249 0.0027 0.0087 0.0081 0.0112 0.0029 0.0094 0.0023 0.0019 0.0012 0.0015 0.0015 0.0018 0.0136 0.0016 0.0106 0.0007 0.0015
Peroxisomes 0.0111 0.0071 0.0015 0.0006 0.0026 0.0045 0.0277 0.0007 0.0079 0.015 0.0025 0.0064 0.0121 0.0003 0.0097 0.0014 0.007 0.0034
Punctate Nuclear 0.0159 0.0027 0.0181 0.0265 0.0153 0.0091 0.0092 0.0024 0.002 0.0016 0.0011 0.0007 0.0089 0.0135 0.0018 0.025 0.0271 0.0106
Vacuole 0.0063 0.0013 0.0018 0.001 0.0028 0.0013 0.0031 0.0032 0.0052 0.0055 0.0015 0.0018 0.0032 0.0022 0.0018 0.0027 0.0009 0.001
Vacuole Periphery 0.0022 0.0001 0.0005 0.0004 0.0019 0.0006 0.0008 0.0004 0.0007 0.0033 0.0005 0.0006 0.0015 0.0003 0.0007 0.0009 0.0002 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.3151 9.5027 18.3336 54.5317 27.5648 8.4994 6.7257 7.7818 34.5273 31.9874
Translational Efficiency 0.778 0.2966 0.6123 0.4701 0.4634 0.6789 0.7311 0.6986 0.7359 0.434

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1414 967 1726 83 1232 2407 1244 1088 2646 3374 2970 1171

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 641.89 763.74 821.34 933.19 649.72 764.42 875.69 833.81 645.54 764.23 844.10 840.85
Standard Deviation 81.10 89.41 92.75 103.76 74.24 87.57 98.82 109.64 78.08 88.10 99.04 112.17
Intensity Change Log 2 — 0.250755 0.355653 0.539845 — 0.234547 0.430602 0.359901 — 0.242625 0.393842 0.452132

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000181 0.000518 0.000233 0.016276 0.000066 0.000174 0.000482 0.000597 0.000128 0.000272 0.000338 0.001708
Bud Neck 0.018806 0.024650 0.005093 0.002130 0.005443 0.025498 0.002562 0.005610 0.012584 0.025255 0.004033 0.005363
Bud Site 0.002697 0.011676 0.009776 0.017630 0.001836 0.012498 0.005260 0.016960 0.002296 0.012262 0.007885 0.017008
Cell Periphery 0.000366 0.000228 0.000212 0.000478 0.000187 0.000422 0.000268 0.000304 0.000282 0.000367 0.000236 0.000316
Cytoplasm 0.417549* 0.404782* 0.416322* 0.195033* 0.534950* 0.497269* 0.509024* 0.479325* 0.472212* 0.470762* 0.455151* 0.459175*
Cytoplasmic Foci 0.276857* 0.298968* 0.012556 0.144583 0.218371* 0.120915 0.041936 0.098290 0.249626* 0.171946 0.024862 0.101571
Eisosomes 0.000687 0.000272 0.000035 0.000324 0.000179 0.000336 0.000083 0.000136 0.000450 0.000318 0.000055 0.000149
Endoplasmic Reticulum 0.004070 0.003214 0.006159 0.011610 0.001211 0.002058 0.008806 0.010807 0.002738 0.002390 0.007268 0.010864
Endosome 0.012501 0.024909 0.001942 0.027673 0.008408 0.009257 0.006640 0.011465 0.010595 0.013743 0.003910 0.012614
Golgi 0.006191 0.006619 0.000082 0.000866 0.001271 0.001357 0.001061 0.001391 0.003900 0.002865 0.000492 0.001354
Lipid Particles 0.037542 0.013185 0.001498 0.020575 0.011534 0.008077 0.004970 0.010558 0.025433 0.009541 0.002952 0.011268
Mitochondria 0.009972 0.007445 0.000527 0.000151 0.002765 0.005211 0.001115 0.000357 0.006616 0.005851 0.000773 0.000342
Mitotic Spindle 0.000087 0.001986 0.000933 0.000370 0.001277 0.004660 0.004308 0.004165 0.000641 0.003893 0.002346 0.003896
None 0.002749 0.002105 0.002968 0.000413 0.004107 0.005056 0.003892 0.001676 0.003382 0.004210 0.003355 0.001586
Nuclear Periphery 0.001049 0.000540 0.002736 0.002261 0.000787 0.000725 0.002756 0.001708 0.000927 0.000672 0.002744 0.001747
Nuclear Periphery Foci 0.000452 0.000933 0.002786 0.033350 0.000672 0.000341 0.009323 0.010963 0.000554 0.000511 0.005524 0.012550
Nucleolus 0.001097 0.001295 0.000342 0.000959 0.001167 0.001339 0.000483 0.000610 0.001130 0.001326 0.000401 0.000634
Nucleus 0.155258* 0.108144 0.485338* 0.107592 0.169175* 0.247549* 0.255697* 0.134540* 0.161738* 0.207595* 0.389152* 0.132630*
Peroxisomes 0.008153 0.011774 0.000154 0.007090 0.002633 0.003780 0.000443 0.003304 0.005583 0.006071 0.000275 0.003572
Vacuole 0.042373 0.074770 0.050001 0.409986* 0.033048 0.052516 0.139936 0.206157* 0.038031 0.058894 0.087671 0.220604*
Vacuole Periphery 0.001364 0.001985 0.000305 0.000649 0.000914 0.000962 0.000956 0.001079 0.001154 0.001255 0.000578 0.001048

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.00 -2.36 -1.04 -1.00 -1.03 -3.81 -3.09 -6.13 -4.65 -0.13 -4.26 -3.78 -1.66 -1.43 -1.26
Bud Neck -2.28 8.12 11.11 10.95 5.01 -12.08 4.03 0.21 12.08 -3.55 -8.36 8.64 7.54 14.69 -0.78
Bud Site -7.30 -4.00 -1.31 -0.37 -0.67 -9.11 -4.75 -6.19 -0.83 -4.02 -11.01 -5.50 -6.10 -1.02 -3.18
Cell Periphery 5.48 6.26 -0.37 -3.20 -3.57 -4.63 -4.73 -3.60 2.82 -0.62 -2.19 3.59 0.24 2.16 -2.24
Cytoplasm 1.30 3.34 10.23 9.30 8.83 4.24 6.34 8.18 5.27 2.11 0.24 7.29 4.77 4.66 -0.49
Cytoplasmic Foci -2.42 48.79 10.73 11.60 -6.75 15.41 31.10 20.02 6.98 -12.48 15.20 56.74 30.92 17.18 -18.70
Eisosomes 7.69 13.83 7.79 0.09 -8.50 -2.97 8.35 4.15 4.01 -5.70 2.97 15.06 11.73 4.73 -11.80
Endoplasmic Reticulum 1.14 -4.29 -2.50 -2.86 -1.28 -2.23 -11.20 -7.18 -6.24 -0.64 0.87 -10.57 -6.09 -6.41 -1.63
Endosome -6.37 11.85 -3.38 0.84 -7.35 -0.73 2.87 -1.67 -0.96 -4.83 -3.40 11.41 -0.47 2.67 -9.98
Golgi -0.38 13.07 10.07 6.57 -1.99 -0.34 1.30 0.47 0.61 -0.40 2.75 11.65 5.27 3.32 -1.25
Lipid Particles 10.21 16.77 6.21 -0.86 -5.08 3.64 8.15 2.08 -0.91 -4.56 12.02 18.26 10.58 0.02 -7.50
Mitochondria 1.50 7.83 8.15 5.83 7.37 -2.38 2.05 2.93 7.97 1.47 0.83 7.69 8.36 9.80 2.29
Mitotic Spindle -2.30 -6.26 -1.29 0.09 -0.65 -2.78 -1.87 -2.09 0.32 -0.11 -4.11 -2.54 -2.98 -0.08 -1.47
None 0.97 0.05 4.10 2.24 4.53 -1.17 1.02 5.69 4.87 2.84 -1.39 0.93 5.81 4.99 4.24
Nuclear Periphery 3.68 -10.52 -3.49 -4.65 0.76 0.24 -7.21 -4.09 -6.07 3.97 1.95 -12.30 -5.09 -7.35 5.61
Nuclear Periphery Foci -4.05 -8.36 -2.84 -2.78 -2.55 4.07 -11.75 -9.83 -10.19 -1.41 0.76 -14.15 -9.87 -9.91 -5.30
Nucleolus -1.06 3.92 1.47 2.53 -2.01 -0.24 4.17 3.57 3.70 -1.18 -0.87 5.74 4.13 4.71 -2.54
Nucleus 8.20 -42.79 2.92 0.01 22.38 -11.92 -11.26 4.96 16.81 15.30 -9.56 -38.47 5.02 12.56 37.74
Peroxisomes -3.15 12.78 1.29 2.47 -1.53 -1.93 5.13 -0.01 1.39 -3.28 -0.85 13.48 3.54 4.18 -3.79
Vacuole -8.91 -8.45 -13.71 -12.58 -12.69 -7.92 -23.87 -29.05 -26.42 -7.86 -10.64 -23.07 -30.88 -27.76 -18.86
Vacuole Periphery -2.60 6.60 2.70 4.05 -0.96 -0.22 0.53 0.25 0.39 -0.19 -0.69 3.80 1.00 1.28 -0.90
ORF
Human Ortholog
Description Putative protein of unknown function; expression depends on Swi5p; GFP-fusion protein localizes to the endoplasmic reticulum; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
Localization
Cell Percentages cytoplasm (32%), ER (6%), mixed (39%)
Cell Cycle Regulation No
Subcompartmental Group N/A

YNL046W

YNL046W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ynl046w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available