Standard name | |
---|---|
Human Ortholog | |
Description | Component of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 8 | 0 | 2 | 2 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 9 | 13 | 7 | 16 | 6 | 10 | 0 | 3 | 6 | 0 | 5 | 4 |
Bud | 1 | 0 | 4 | 4 | 5 | 10 | 14 | 18 | 14 | 18 | 9 | 16 | 1 | 0 | 1 | 3 | 8 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 2 | 3 | 5 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 1 | 1 | 2 | 3 | 4 | 0 | 2 | 0 | 5 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 3 | 2 | 16 | 15 | 15 | 15 | 12 | 11 | 6 | 13 | 12 | 6 | 14 | 24 | 29 | 14 | 17 | 0 | 0 | 0 | 0 | 4 | 2 |
Endoplasmic Reticulum | 8 | 13 | 2 | 6 | 0 | 1 | 6 | 0 | 2 | 3 | 4 | 3 | 11 | 12 | 10 | 11 | 8 | 11 | 0 | 1 | 0 | 0 | 2 | 1 |
Endosome | 1 | 3 | 36 | 9 | 31 | 3 | 3 | 27 | 3 | 0 | 1 | 1 | 10 | 41 | 31 | 45 | 27 | 17 | 23 | 16 | 16 | 3 | 10 | 18 |
Golgi | 18 | 50 | 19 | 17 | 4 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 32 | 130 | 127 | 148 | 78 | 90 | 124 | 122 | 120 | 35 | 115 | 141 |
Mitochondria | 168 | 135 | 120 | 148 | 161 | 267 | 371 | 283 | 261 | 301 | 302 | 320 | 24 | 39 | 33 | 22 | 18 | 14 | 20 | 18 | 8 | 17 | 30 | 28 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 2 | 1 | 1 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 1 | 4 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 2 | 0 | 2 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | 1 | 1 | 1 | 0 | 3 | 1 | 7 | 2 | 2 | 5 | 2 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 15 | 4 | 12 | 3 | 6 | 26 | 2 | 3 | 2 | 3 | 1 | 0 | 0 | 2 | 0 | 0 | 4 | 1 | 17 | 1 | 1 | 1 |
SpindlePole | 0 | 0 | 4 | 0 | 1 | 3 | 6 | 14 | 7 | 4 | 3 | 3 | 2 | 5 | 4 | 9 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 7 | 8 | 4 | 2 | 5 | 7 | 8 | 10 | 12 | 9 | 7 | 11 | 5 | 22 | 23 | 43 | 18 | 28 | 0 | 3 | 0 | 4 | 5 | 7 |
Unique Cell Count | 171 | 155 | 154 | 175 | 198 | 285 | 390 | 325 | 281 | 308 | 317 | 336 | 72 | 221 | 207 | 283 | 156 | 163 | 175 | 170 | 172 | 66 | 179 | 208 |
Labelled Cell Count | 210 | 221 | 209 | 215 | 236 | 313 | 439 | 407 | 320 | 353 | 355 | 380 | 102 | 280 | 261 | 338 | 183 | 194 | 175 | 170 | 172 | 66 | 179 | 208 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.1 | 7.5 | 7.3 | 6.4 | 6.4 | 5.2 | 5.0 | 5.0 | 4.8 | 4.5 | 4.1 | 4.2 | 8.2 | 8.5 | 8.3 | 8.6 | 8.7 | 8.7 | 8.7 | 8.7 | 8.9 |
Std Deviation (1e-4) | 0.8 | 1.2 | 1.3 | 1.4 | 2.1 | 1.4 | 1.2 | 1.5 | 1.1 | 1.0 | 1.2 | 1.2 | 1.0 | 1.0 | 1.1 | 1.5 | 1.6 | 1.5 | 0.8 | 1.2 | 1.2 |
Intensity Change (Log2) | – | – | – | -0.18 | -0.18 | -0.49 | -0.55 | -0.53 | -0.62 | -0.7 | -0.83 | -0.79 | 0.17 | 0.21 | 0.18 | 0.23 | 0.25 | 0.25 | 0.25 | 0.25 | 0.29 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | 0 | 0 | 0 | 0.6 | 1.4 | 0 | – | – | – | 0 | 0 | 0 | 0 | 1.2 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 2.1 | 1.9 | 0 | 0 | 2.4 |
Endosome | -4.8 | -1.8 | -7.8 | -9.2 | -4.6 | 0 | – | – | – | -1.7 | -1.1 | -2.0 | -1.9 | -1.3 | -3.1 |
Golgi | -0.8 | -3.9 | -6.1 | -6.7 | -6.1 | 0 | – | – | – | 5.4 | 9.0 | 9.4 | 8.2 | 7.2 | 8.0 |
Mitochondria | 1.5 | 0.8 | 4.9 | 6.1 | 2.6 | 0 | – | – | – | -6.5 | -11.6 | -11.8 | -15.0 | -11.8 | -12.5 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | -2.9 | -1.3 | -4.4 | -4.5 | -0.6 | 0 | – | – | – | -2.3 | -4.7 | -4.6 | -4.7 | -4.0 | -4.1 |
SpindlePole | 0 | 0 | 0 | 0 | 0.9 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 3.1 | 0 | 3.0 | 0 | 0 |
Cytoplasm | 3.1 | 2.7 | 2.1 | 0 | 0 | 0 | – | – | – | 0 | 2.4 | 3.7 | 3.5 | 3.1 | 3.4 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0.9 | – | – | – | 0 | 2.8 | 3.0 | 4.1 | 3.1 | 4.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.8655 | 2.4263 | 2.1211 | 1.5299 | 1.6733 | 2.1577 | 2.1217 | 2.5369 | 2.4867 | 2.2165 | 1.9478 | 2.4722 | 1.5618 | 1.6148 | 1.9847 | 2.0302 | 1.8127 | 2.033 |
Actin | 0.0232 | 0.0007 | 0.018 | 0.0098 | 0.0064 | 0.0035 | 0.0371 | 0.0063 | 0.0093 | 0.0051 | 0.0074 | 0.0164 | 0.0561 | 0.0102 | 0.008 | 0.0014 | 0.0367 | 0.0057 |
Bud | 0.0002 | 0.0001 | 0.0004 | 0.0003 | 0.0002 | 0.0001 | 0.0007 | 0.0002 | 0.0008 | 0.0004 | 0.0002 | 0.0002 | 0.0005 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0001 |
Bud Neck | 0.0004 | 0.0002 | 0.0005 | 0.0011 | 0.0007 | 0.0003 | 0.0044 | 0.0003 | 0.0005 | 0.0008 | 0.0004 | 0.0006 | 0.0023 | 0.0004 | 0.0004 | 0.0002 | 0.0008 | 0.0004 |
Bud Periphery | 0.0002 | 0.0001 | 0.0005 | 0.0003 | 0.0003 | 0.0001 | 0.0009 | 0.0002 | 0.0012 | 0.0004 | 0.0002 | 0.0003 | 0.0007 | 0.0001 | 0.0001 | 0.0002 | 0.0004 | 0.0001 |
Bud Site | 0.0005 | 0.0002 | 0.0012 | 0.0008 | 0.0007 | 0.0002 | 0.0121 | 0.0016 | 0.0013 | 0.0021 | 0.0004 | 0.0007 | 0.0061 | 0.0005 | 0.0002 | 0.0001 | 0.0003 | 0.0002 |
Cell Periphery | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 |
Cytoplasm | 0.0033 | 0.0052 | 0.0047 | 0.0032 | 0.0029 | 0.0018 | 0.0058 | 0.0038 | 0.0051 | 0.0036 | 0.0042 | 0.0025 | 0.0059 | 0.0044 | 0.0024 | 0.0021 | 0.0011 | 0.0014 |
Cytoplasmic Foci | 0.0865 | 0.1151 | 0.0862 | 0.0981 | 0.0783 | 0.0756 | 0.091 | 0.106 | 0.0846 | 0.0823 | 0.058 | 0.0792 | 0.11 | 0.1003 | 0.0815 | 0.079 | 0.0913 | 0.0541 |
Eisosomes | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0004 | 0.0001 | 0.0001 | 0 | 0 | 0.0002 | 0.0012 | 0 | 0 | 0 | 0.0006 | 0.0001 |
Endoplasmic Reticulum | 0.0016 | 0.0006 | 0.0011 | 0.0005 | 0.0023 | 0.0008 | 0.0087 | 0.0007 | 0.0024 | 0.0007 | 0.0013 | 0.0014 | 0.0038 | 0.001 | 0.0008 | 0.001 | 0.0053 | 0.0008 |
Endosome | 0.2772 | 0.3011 | 0.2479 | 0.2801 | 0.2221 | 0.2002 | 0.2885 | 0.3381 | 0.2963 | 0.3657 | 0.343 | 0.2594 | 0.2435 | 0.3001 | 0.3177 | 0.3985 | 0.3618 | 0.2206 |
Golgi | 0.4812 | 0.4965 | 0.5266 | 0.4562 | 0.5359 | 0.611 | 0.4346 | 0.4988 | 0.5318 | 0.5082 | 0.5375 | 0.5689 | 0.4046 | 0.5434 | 0.53 | 0.4563 | 0.4379 | 0.6544 |
Lipid Particles | 0.0219 | 0.0103 | 0.0132 | 0.0123 | 0.0292 | 0.0186 | 0.0407 | 0.0151 | 0.0068 | 0.0059 | 0.0121 | 0.0148 | 0.0392 | 0.0088 | 0.0053 | 0.0077 | 0.0257 | 0.0211 |
Mitochondria | 0.0561 | 0.0583 | 0.0645 | 0.0779 | 0.0568 | 0.0682 | 0.0284 | 0.0136 | 0.0448 | 0.0148 | 0.0192 | 0.041 | 0.0668 | 0.0192 | 0.0445 | 0.0204 | 0.013 | 0.0317 |
None | 0.003 | 0.0002 | 0.006 | 0.0022 | 0.0046 | 0.0002 | 0.0023 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.003 | 0.0001 | 0.0001 | 0.0001 | 0.0012 | 0.0001 |
Nuclear Periphery | 0.003 | 0.0001 | 0.0005 | 0.0002 | 0.0106 | 0.0002 | 0.0067 | 0.0002 | 0.0007 | 0.0002 | 0.0003 | 0.0009 | 0.002 | 0.0001 | 0.0002 | 0.0002 | 0.0054 | 0.0002 |
Nucleolus | 0.0002 | 0 | 0.0001 | 0.0125 | 0.0023 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0005 | 0.0002 | 0 | 0 | 0.0001 | 0 |
Nucleus | 0.0017 | 0 | 0.0002 | 0.0021 | 0.0051 | 0.0001 | 0.0008 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0009 | 0.0001 | 0.0001 | 0 | 0.0004 | 0 |
Peroxisomes | 0.0325 | 0.0074 | 0.0222 | 0.0303 | 0.0131 | 0.0151 | 0.0228 | 0.0109 | 0.0062 | 0.0034 | 0.0089 | 0.0084 | 0.0419 | 0.0045 | 0.0034 | 0.0261 | 0.0034 | 0.0048 |
Punctate Nuclear | 0.0016 | 0.0001 | 0.0013 | 0.0008 | 0.0232 | 0.0006 | 0.0019 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0006 | 0.0037 | 0.0001 | 0.0001 | 0.0001 | 0.001 | 0.0001 |
Vacuole | 0.0026 | 0.0015 | 0.0025 | 0.0091 | 0.0026 | 0.0013 | 0.0072 | 0.0017 | 0.0031 | 0.0024 | 0.003 | 0.0015 | 0.0038 | 0.0037 | 0.0021 | 0.0024 | 0.0058 | 0.0013 |
Vacuole Periphery | 0.0027 | 0.0023 | 0.0023 | 0.002 | 0.0026 | 0.0019 | 0.0042 | 0.0019 | 0.004 | 0.0035 | 0.0034 | 0.0027 | 0.0027 | 0.0023 | 0.0029 | 0.0038 | 0.0074 | 0.0025 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 11.8352 | 6.5107 | 12.682 | 15.3359 | 12.9841 | 11.8286 | 11.9317 | 13.8846 | 11.5963 | 16.6501 |
Translational Efficiency | 1.0681 | 1.8468 | 1.036 | 0.8299 | 0.8618 | 1.258 | 1.0229 | 1.0771 | 1.3217 | 1.064 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
203 | 176 | 145 | 1116 | 2108 | 1978 | 1047 | 1486 | 2311 | 2154 | 1192 | 2602 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 835.01 | 909.50 | 1099.59 | 952.48 | 861.28 | 907.88 | 1012.54 | 1035.20 | 858.97 | 908.01 | 1023.13 | 999.72 |
Standard Deviation | 100.95 | 109.43 | 140.35 | 131.08 | 105.95 | 115.11 | 133.63 | 140.62 | 105.78 | 114.65 | 137.44 | 142.62 |
Intensity Change Log 2 | — | 0.123280 | 0.397100 | 0.189895 | — | 0.076019 | 0.233425 | 0.265355 | — | 0.099477 | 0.316316 | 0.228703 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001535 | 0.002205 | 0.006254 | 0.021359 | 0.000997 | 0.001196 | 0.006242 | 0.014273 | 0.001044 | 0.001278 | 0.006243 | 0.017312 |
Bud Neck | 0.000238 | 0.000283 | 0.001063 | 0.001236 | 0.000060 | 0.000096 | 0.000416 | 0.001338 | 0.000076 | 0.000111 | 0.000495 | 0.001295 |
Bud Site | 0.002490 | 0.002167 | 0.005006 | 0.019224 | 0.000489 | 0.000915 | 0.006019 | 0.016734 | 0.000664 | 0.001018 | 0.005896 | 0.017802 |
Cell Periphery | 0.000094 | 0.000143 | 0.000137 | 0.000138 | 0.000063 | 0.000049 | 0.000075 | 0.000069 | 0.000065 | 0.000057 | 0.000082 | 0.000098 |
Cytoplasm | 0.001150 | 0.000606 | 0.001899 | 0.009251 | 0.000518 | 0.001275 | 0.002657 | 0.009812 | 0.000573 | 0.001221 | 0.002565 | 0.009571 |
Cytoplasmic Foci | 0.175424 | 0.181975* | 0.269748* | 0.250915* | 0.137436 | 0.149088 | 0.283843* | 0.275104* | 0.140773 | 0.151775 | 0.282128* | 0.264729* |
Eisosomes | 0.000096 | 0.000073 | 0.000052 | 0.000146 | 0.000046 | 0.000032 | 0.000053 | 0.000081 | 0.000050 | 0.000036 | 0.000053 | 0.000109 |
Endoplasmic Reticulum | 0.000305 | 0.000296 | 0.002479 | 0.003281 | 0.000401 | 0.000284 | 0.003273 | 0.000681 | 0.000393 | 0.000285 | 0.003176 | 0.001796 |
Endosome | 0.166038 | 0.200239* | 0.222076* | 0.189977 | 0.111494 | 0.151126 | 0.155754 | 0.199714 | 0.116285 | 0.155139 | 0.163822 | 0.195538 |
Golgi | 0.586805* | 0.587128* | 0.325950* | 0.387326* | 0.677019* | 0.642734* | 0.468533* | 0.370837* | 0.669095* | 0.638191* | 0.451188* | 0.377909* |
Lipid Particles | 0.003587 | 0.004488 | 0.039645 | 0.003758 | 0.004723 | 0.003018 | 0.005347 | 0.003243 | 0.004623 | 0.003138 | 0.009519 | 0.003464 |
Mitochondria | 0.041961 | 0.010155 | 0.004237 | 0.016431 | 0.045603 | 0.030970 | 0.008959 | 0.014964 | 0.045283 | 0.029269 | 0.008384 | 0.015593 |
Mitotic Spindle | 0.005286 | 0.000600 | 0.032557 | 0.021466 | 0.000973 | 0.001288 | 0.011787 | 0.027936 | 0.001352 | 0.001232 | 0.014313 | 0.025161 |
None | 0.000031 | 0.000011 | 0.000091 | 0.000025 | 0.000048 | 0.000040 | 0.000022 | 0.000023 | 0.000046 | 0.000038 | 0.000031 | 0.000024 |
Nuclear Periphery | 0.000030 | 0.000012 | 0.000564 | 0.000075 | 0.000087 | 0.000031 | 0.000139 | 0.000097 | 0.000082 | 0.000029 | 0.000191 | 0.000088 |
Nuclear Periphery Foci | 0.000225 | 0.000094 | 0.011930 | 0.000569 | 0.000361 | 0.000142 | 0.001227 | 0.000441 | 0.000349 | 0.000138 | 0.002529 | 0.000496 |
Nucleolus | 0.000168 | 0.000004 | 0.001248 | 0.000023 | 0.000039 | 0.000006 | 0.000023 | 0.000026 | 0.000050 | 0.000006 | 0.000172 | 0.000025 |
Nucleus | 0.000268 | 0.000008 | 0.000468 | 0.000244 | 0.000025 | 0.000016 | 0.000082 | 0.000197 | 0.000046 | 0.000015 | 0.000129 | 0.000217 |
Peroxisomes | 0.007023 | 0.002037 | 0.012167 | 0.017912 | 0.001400 | 0.001479 | 0.004557 | 0.022330 | 0.001894 | 0.001524 | 0.005483 | 0.020435 |
Vacuole | 0.002475 | 0.003697 | 0.040049 | 0.046057 | 0.005012 | 0.006580 | 0.028670 | 0.035758 | 0.004789 | 0.006345 | 0.030054 | 0.040175 |
Vacuole Periphery | 0.004772 | 0.003780 | 0.022381 | 0.010586 | 0.013206 | 0.009634 | 0.012325 | 0.006340 | 0.012465 | 0.009155 | 0.013548 | 0.008161 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.95 | -1.17 | -7.54 | -7.21 | -3.17 | -0.48 | -3.70 | -7.33 | -7.12 | -3.51 | -0.61 | -3.88 | -10.65 | -10.32 | -5.48 |
Bud Neck | -0.23 | -3.54 | -6.45 | -5.43 | -1.09 | -1.58 | -7.32 | -6.69 | -6.48 | -4.76 | -1.35 | -8.26 | -10.20 | -9.82 | -6.43 |
Bud Site | 0.15 | -1.26 | -6.87 | -8.62 | -7.94 | -2.43 | -8.93 | -11.16 | -10.81 | -6.93 | -1.45 | -9.10 | -15.48 | -15.14 | -9.90 |
Cell Periphery | -0.86 | -1.26 | -1.94 | 0.10 | 0.00 | 1.28 | -1.17 | -0.69 | -2.31 | 0.65 | 0.79 | -1.68 | -3.24 | -3.99 | -1.62 |
Cytoplasm | 0.85 | -1.12 | -6.99 | -8.28 | -6.99 | -1.66 | -6.54 | -10.42 | -8.42 | -7.76 | -1.55 | -6.75 | -13.29 | -10.38 | -9.88 |
Cytoplasmic Foci | -0.49 | -5.84 | -7.07 | -6.25 | 1.42 | -3.40 | -27.75 | -27.63 | -24.51 | 1.46 | -3.29 | -28.24 | -31.12 | -27.22 | 3.31 |
Eisosomes | 0.93 | 1.96 | -1.89 | -3.35 | -4.99 | 2.70 | -1.15 | -5.27 | -9.23 | -4.43 | 2.89 | -0.44 | -6.56 | -9.15 | -6.55 |
Endoplasmic Reticulum | 0.15 | -4.07 | -3.85 | -3.88 | -0.88 | 1.56 | -3.66 | -1.59 | -2.17 | 3.22 | 1.56 | -4.01 | -4.06 | -4.34 | 1.82 |
Endosome | -2.35 | -3.34 | -2.20 | 0.96 | 2.27 | -10.71 | -10.24 | -17.49 | -9.06 | -7.52 | -10.71 | -10.98 | -19.83 | -9.23 | -6.29 |
Golgi | -0.03 | 10.50 | 12.06 | 11.85 | -2.64 | 5.35 | 24.91 | 37.58* | 33.16 | 10.19 | 5.00 | 27.07 | 43.67* | 38.77 | 9.02 |
Lipid Particles | -0.40 | -3.38 | -0.31 | 0.27 | 3.36 | 1.91 | -0.62 | 1.60 | -0.47 | 2.04 | 1.78 | -3.00 | 1.43 | -0.72 | 3.81 |
Mitochondria | 3.73 | 4.68 | 3.16 | -1.50 | -4.05 | 4.18 | 11.85 | 9.70 | 6.21 | -2.98 | 4.86 | 12.75 | 10.16 | 5.86 | -4.08 |
Mitotic Spindle | 1.26 | -3.01 | -3.56 | -7.53 | 1.07 | -1.07 | -7.03 | -9.85 | -9.72 | -5.29 | 0.34 | -7.57 | -11.96 | -12.16 | -4.57 |
None | 2.16 | -0.88 | 0.64 | -3.87 | 0.95 | 0.32 | 2.40 | 2.12 | 0.65 | -0.53 | 0.39 | 0.86 | 2.22 | 0.59 | 0.71 |
Nuclear Periphery | 1.87 | -3.59 | -4.24 | -6.69 | 3.21 | 1.54 | -1.18 | -0.38 | -3.45 | 1.10 | 1.59 | -2.46 | -0.41 | -5.09 | 2.91 |
Nuclear Periphery Foci | 1.19 | -2.56 | -2.81 | -5.48 | 2.47 | 1.78 | -3.27 | -0.72 | -2.99 | 2.92 | 1.88 | -3.58 | -1.49 | -5.03 | 3.30 |
Nucleolus | 1.04 | -1.04 | 0.91 | -6.25 | 1.17 | 1.13 | 0.55 | 0.41 | -6.88 | -0.69 | 1.48 | -0.96 | 0.82 | -8.82 | 1.14 |
Nucleus | 1.07 | -0.59 | -0.01 | -3.25 | 0.69 | 0.91 | -3.07 | -1.78 | -1.84 | -1.41 | 1.32 | -1.74 | -2.50 | -2.90 | -1.47 |
Peroxisomes | 2.66 | -1.17 | -4.30 | -8.46 | -1.28 | -0.28 | -4.45 | -10.73 | -10.65 | -8.63 | 1.19 | -4.41 | -13.51 | -13.78 | -9.57 |
Vacuole | -1.12 | -6.03 | -13.85 | -13.01 | -1.56 | -2.59 | -10.67 | -13.65 | -12.80 | -2.87 | -2.77 | -12.06 | -18.93 | -17.82 | -4.62 |
Vacuole Periphery | 0.81 | -3.30 | -4.98 | -4.83 | 2.21 | 3.51 | 0.70 | 7.72 | 4.26 | 5.67 | 3.53 | -0.84 | 5.10 | 1.39 | 4.67 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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