Standard name
Human Ortholog
Description Component of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.05 0 0 0 0 0 0 0 0 0 0 0.12 0.06 0 0.06 0 0.06 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0.06 0.05 0.06 0 0.05 0 0 0 0 0.05 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.09 0.08 0.05 0 0 0 0 0 0 0.08 0.06 0.12 0.1 0.09 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0.05 0.08 0 0 0 0 0 0 0 0 0 0 0.15 0.05 0.05 0 0.05 0.07 0 0 0 0 0 0
Endosome 0 0 0.23 0.05 0.16 0 0 0.08 0 0 0 0 0.14 0.19 0.15 0.16 0.17 0.1 0.13 0.1 0.09 0.06 0.06 0.09
Golgi 0.11 0.32 0.12 0.1 0 0 0 0 0 0 0 0 0.44 0.59 0.61 0.52 0.5 0.55 0.71 0.72 0.7 0.54 0.65 0.68
Mitochondria 0.98 0.87 0.78 0.85 0.81 0.94 0.95 0.87 0.93 0.98 0.95 0.95 0.33 0.18 0.16 0.08 0.12 0.09 0.11 0.11 0.05 0.26 0.17 0.14
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.1 0 0.06 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 0 0 0 0 0 0 0 0 0 0 0.07 0.1 0.11 0.15 0.12 0.17 0 0 0 0.07 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 8 0 2 2 0 3 0 0 0 0 0 9 13 7 16 6 10 0 3 6 0 5 4
Bud 1 0 4 4 5 10 14 18 14 18 9 16 1 0 1 3 8 1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 3 2 2 3 5 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 2 0 0 0 1 1 2 3 4 0 2 0 5 1 1 0 0 0 0 0 0
Cytoplasm 4 3 2 16 15 15 15 12 11 6 13 12 6 14 24 29 14 17 0 0 0 0 4 2
Endoplasmic Reticulum 8 13 2 6 0 1 6 0 2 3 4 3 11 12 10 11 8 11 0 1 0 0 2 1
Endosome 1 3 36 9 31 3 3 27 3 0 1 1 10 41 31 45 27 17 23 16 16 3 10 18
Golgi 18 50 19 17 4 0 1 1 0 0 0 0 32 130 127 148 78 90 124 122 120 35 115 141
Mitochondria 168 135 120 148 161 267 371 283 261 301 302 320 24 39 33 22 18 14 20 18 8 17 30 28
Nucleus 0 0 0 0 0 0 1 5 2 1 1 1 0 0 1 1 1 1 0 0 0 0 0 0
Nuclear Periphery 0 0 1 4 0 1 1 1 1 1 0 1 1 2 0 2 1 2 0 1 0 0 0 0
Nucleolus 1 1 1 1 0 3 1 7 2 2 5 2 0 0 0 2 2 0 0 0 0 0 0 0
Peroxisomes 0 0 15 4 12 3 6 26 2 3 2 3 1 0 0 2 0 0 4 1 17 1 1 1
SpindlePole 0 0 4 0 1 3 6 14 7 4 3 3 2 5 4 9 1 2 0 0 0 0 0 0
Vac/Vac Membrane 7 8 4 2 5 7 8 10 12 9 7 11 5 22 23 43 18 28 0 3 0 4 5 7
Unique Cell Count 171 155 154 175 198 285 390 325 281 308 317 336 72 221 207 283 156 163 175 170 172 66 179 208
Labelled Cell Count 210 221 209 215 236 313 439 407 320 353 355 380 102 280 261 338 183 194 175 170 172 66 179 208


Early Golgi, Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.1 7.5 7.3 6.4 6.4 5.2 5.0 5.0 4.8 4.5 4.1 4.2 8.2 8.5 8.3 8.6 8.7 8.7 8.7 8.7 8.9
Std Deviation (1e-4) 0.8 1.2 1.3 1.4 2.1 1.4 1.2 1.5 1.1 1.0 1.2 1.2 1.0 1.0 1.1 1.5 1.6 1.5 0.8 1.2 1.2
Intensity Change (Log2) – – – -0.18 -0.18 -0.49 -0.55 -0.53 -0.62 -0.7 -0.83 -0.79 0.17 0.21 0.18 0.23 0.25 0.25 0.25 0.25 0.29


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud 0 0 0 0.6 1.4 0 – – – 0 0 0 0 1.2 0
Bud Neck 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum 0 0 0 0 0 0 – – – 0 2.1 1.9 0 0 2.4
Endosome -4.8 -1.8 -7.8 -9.2 -4.6 0 – – – -1.7 -1.1 -2.0 -1.9 -1.3 -3.1
Golgi -0.8 -3.9 -6.1 -6.7 -6.1 0 – – – 5.4 9.0 9.4 8.2 7.2 8.0
Mitochondria 1.5 0.8 4.9 6.1 2.6 0 – – – -6.5 -11.6 -11.8 -15.0 -11.8 -12.5
Nuclear Periphery 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes -2.9 -1.3 -4.4 -4.5 -0.6 0 – – – -2.3 -4.7 -4.6 -4.7 -4.0 -4.1
SpindlePole 0 0 0 0 0.9 0 – – – 0 0 0 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches 0 0 0 0 0 0 – – – 0 3.1 0 3.0 0 0
Cytoplasm 3.1 2.7 2.1 0 0 0 – – – 0 2.4 3.7 3.5 3.1 3.4
Nucleus 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0.9 – – – 0 2.8 3.0 4.1 3.1 4.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.8655 2.4263 2.1211 1.5299 1.6733 2.1577 2.1217 2.5369 2.4867 2.2165 1.9478 2.4722 1.5618 1.6148 1.9847 2.0302 1.8127 2.033
Actin 0.0232 0.0007 0.018 0.0098 0.0064 0.0035 0.0371 0.0063 0.0093 0.0051 0.0074 0.0164 0.0561 0.0102 0.008 0.0014 0.0367 0.0057
Bud 0.0002 0.0001 0.0004 0.0003 0.0002 0.0001 0.0007 0.0002 0.0008 0.0004 0.0002 0.0002 0.0005 0.0002 0.0002 0.0002 0.0002 0.0001
Bud Neck 0.0004 0.0002 0.0005 0.0011 0.0007 0.0003 0.0044 0.0003 0.0005 0.0008 0.0004 0.0006 0.0023 0.0004 0.0004 0.0002 0.0008 0.0004
Bud Periphery 0.0002 0.0001 0.0005 0.0003 0.0003 0.0001 0.0009 0.0002 0.0012 0.0004 0.0002 0.0003 0.0007 0.0001 0.0001 0.0002 0.0004 0.0001
Bud Site 0.0005 0.0002 0.0012 0.0008 0.0007 0.0002 0.0121 0.0016 0.0013 0.0021 0.0004 0.0007 0.0061 0.0005 0.0002 0.0001 0.0003 0.0002
Cell Periphery 0.0001 0 0.0001 0.0002 0.0001 0 0.0004 0 0.0001 0.0001 0.0001 0.0001 0.0006 0.0001 0.0001 0 0.0002 0.0001
Cytoplasm 0.0033 0.0052 0.0047 0.0032 0.0029 0.0018 0.0058 0.0038 0.0051 0.0036 0.0042 0.0025 0.0059 0.0044 0.0024 0.0021 0.0011 0.0014
Cytoplasmic Foci 0.0865 0.1151 0.0862 0.0981 0.0783 0.0756 0.091 0.106 0.0846 0.0823 0.058 0.0792 0.11 0.1003 0.0815 0.079 0.0913 0.0541
Eisosomes 0.0002 0 0.0001 0.0001 0.0001 0 0.0004 0.0001 0.0001 0 0 0.0002 0.0012 0 0 0 0.0006 0.0001
Endoplasmic Reticulum 0.0016 0.0006 0.0011 0.0005 0.0023 0.0008 0.0087 0.0007 0.0024 0.0007 0.0013 0.0014 0.0038 0.001 0.0008 0.001 0.0053 0.0008
Endosome 0.2772 0.3011 0.2479 0.2801 0.2221 0.2002 0.2885 0.3381 0.2963 0.3657 0.343 0.2594 0.2435 0.3001 0.3177 0.3985 0.3618 0.2206
Golgi 0.4812 0.4965 0.5266 0.4562 0.5359 0.611 0.4346 0.4988 0.5318 0.5082 0.5375 0.5689 0.4046 0.5434 0.53 0.4563 0.4379 0.6544
Lipid Particles 0.0219 0.0103 0.0132 0.0123 0.0292 0.0186 0.0407 0.0151 0.0068 0.0059 0.0121 0.0148 0.0392 0.0088 0.0053 0.0077 0.0257 0.0211
Mitochondria 0.0561 0.0583 0.0645 0.0779 0.0568 0.0682 0.0284 0.0136 0.0448 0.0148 0.0192 0.041 0.0668 0.0192 0.0445 0.0204 0.013 0.0317
None 0.003 0.0002 0.006 0.0022 0.0046 0.0002 0.0023 0.0001 0.0003 0.0001 0.0001 0.0002 0.003 0.0001 0.0001 0.0001 0.0012 0.0001
Nuclear Periphery 0.003 0.0001 0.0005 0.0002 0.0106 0.0002 0.0067 0.0002 0.0007 0.0002 0.0003 0.0009 0.002 0.0001 0.0002 0.0002 0.0054 0.0002
Nucleolus 0.0002 0 0.0001 0.0125 0.0023 0.0001 0.0003 0.0001 0.0001 0.0001 0 0.0001 0.0005 0.0002 0 0 0.0001 0
Nucleus 0.0017 0 0.0002 0.0021 0.0051 0.0001 0.0008 0.0001 0.0002 0.0001 0.0001 0.0001 0.0009 0.0001 0.0001 0 0.0004 0
Peroxisomes 0.0325 0.0074 0.0222 0.0303 0.0131 0.0151 0.0228 0.0109 0.0062 0.0034 0.0089 0.0084 0.0419 0.0045 0.0034 0.0261 0.0034 0.0048
Punctate Nuclear 0.0016 0.0001 0.0013 0.0008 0.0232 0.0006 0.0019 0.0002 0.0002 0.0001 0.0002 0.0006 0.0037 0.0001 0.0001 0.0001 0.001 0.0001
Vacuole 0.0026 0.0015 0.0025 0.0091 0.0026 0.0013 0.0072 0.0017 0.0031 0.0024 0.003 0.0015 0.0038 0.0037 0.0021 0.0024 0.0058 0.0013
Vacuole Periphery 0.0027 0.0023 0.0023 0.002 0.0026 0.0019 0.0042 0.0019 0.004 0.0035 0.0034 0.0027 0.0027 0.0023 0.0029 0.0038 0.0074 0.0025

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.8352 6.5107 12.682 15.3359 12.9841 11.8286 11.9317 13.8846 11.5963 16.6501
Translational Efficiency 1.0681 1.8468 1.036 0.8299 0.8618 1.258 1.0229 1.0771 1.3217 1.064

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Component of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
Localization
Cell Percentages Golgi (15%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Cog5

Cog5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cog5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available