ORF | |
---|---|
Human Ortholog | |
Description | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to vacuole; not an essential gene; YNL058C has a paralog, PRM5, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 5 | 4 | 4 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 0 | 3 | 2 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 10 | 12 | 37 | 40 | 72 | 79 | 121 | 132 | 133 | 180 | 175 | 241 | 82 | 244 | 287 | 15 | 28 | 15 | 3 | 2 | 2 | 1 | 3 | 7 |
Endoplasmic Reticulum | 0 | 0 | 1 | 3 | 2 | 1 | 4 | 1 | 2 | 1 | 1 | 2 | 0 | 1 | 2 | 7 | 4 | 4 | 0 | 0 | 0 | 0 | 1 | 0 |
Endosome | 4 | 5 | 3 | 4 | 18 | 11 | 3 | 4 | 1 | 1 | 0 | 1 | 6 | 0 | 0 | 4 | 3 | 2 | 14 | 13 | 12 | 18 | 11 | 14 |
Golgi | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 4 | 0 | 0 | 0 | 2 | 0 | 0 | 0 |
Mitochondria | 58 | 9 | 8 | 5 | 17 | 24 | 58 | 30 | 44 | 35 | 67 | 40 | 6 | 0 | 0 | 1 | 4 | 5 | 2 | 1 | 4 | 4 | 9 | 8 |
Nucleus | 40 | 30 | 49 | 66 | 68 | 77 | 105 | 83 | 43 | 48 | 38 | 24 | 18 | 6 | 4 | 65 | 47 | 44 | 3 | 3 | 1 | 5 | 3 | 3 |
Nuclear Periphery | 6 | 2 | 0 | 1 | 2 | 1 | 3 | 0 | 0 | 6 | 5 | 3 | 0 | 0 | 0 | 2 | 1 | 1 | 2 | 0 | 0 | 0 | 2 | 1 |
Nucleolus | 4 | 2 | 1 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 5 | 0 | 0 | 0 | 1 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 2 | 0 | 2 | 3 | 5 | 1 | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 4 | 2 | 4 |
Vac/Vac Membrane | 95 | 110 | 83 | 86 | 199 | 207 | 213 | 182 | 178 | 180 | 117 | 137 | 88 | 42 | 21 | 72 | 67 | 80 | 133 | 123 | 159 | 120 | 196 | 181 |
Unique Cell Count | 158 | 142 | 159 | 166 | 325 | 323 | 407 | 340 | 315 | 343 | 309 | 372 | 182 | 275 | 303 | 150 | 132 | 137 | 163 | 151 | 189 | 162 | 241 | 226 |
Labelled Cell Count | 220 | 170 | 184 | 214 | 388 | 403 | 509 | 436 | 406 | 455 | 408 | 454 | 201 | 293 | 315 | 174 | 161 | 156 | 163 | 151 | 189 | 162 | 241 | 226 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.1 | 7.3 | 5.9 | 5.8 | 7.0 | 6.3 | 5.6 | 5.8 | 5.2 | 4.8 | 4.6 | 5.0 | 5.5 | 5.3 | 5.1 | 11.9 | 12.3 | 12.2 | 6.9 | 6.5 | 6.6 |
Std Deviation (1e-4) | 1.5 | 2.3 | 1.5 | 1.5 | 2.0 | 2.1 | 1.9 | 1.8 | 1.6 | 1.4 | 1.4 | 1.6 | 1.4 | 1.4 | 1.5 | 4.0 | 4.7 | 4.4 | 2.1 | 1.6 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.02 | 0.25 | 0.09 | -0.07 | -0.03 | -0.19 | -0.29 | -0.38 | -0.25 | -0.11 | -0.15 | -0.21 | 1.01 | 1.06 | 1.04 | 0.23 | 0.13 | 0.16 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.2 | -0.3 | 0.3 | 1.5 | 3.4 | 4.1 | 6.1 | 6.9 | 8.8 | 4.2 | 13.8 | 15.9 | -3.1 | -0.4 | -2.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 1.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -0.9 | 0.1 | 1.0 | 3.1 | 1.5 | 2.9 | 0 | 0 | 2.1 | -0.8 | 0 | 0 | 0 | -0.9 | -0.6 |
Nucleus | 1.7 | -2.4 | -1.6 | -1.2 | -1.5 | -4.5 | -4.4 | -4.9 | -7.5 | -4.9 | -8.6 | -9.5 | 2.3 | 0.9 | 0.2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -0.1 | 1.9 | 2.5 | 0 | 0.3 | 0.9 | 0.1 | -3.0 | -3.3 | -0.7 | -8.2 | -11.1 | -0.7 | -0.2 | 1.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.7999 | 1.1439 | 0.381 | 0.6398 | 1.3318 | 1.7578 | 0.7843 | 2.0369 | 1.3824 | 1.4655 | 2.7019 | 2.9507 | -0.1899 | 1.587 | 0.344 | 0.049 | 0.4228 | 2.1835 |
Actin | 0.0261 | 0 | 0.0073 | 0.0001 | 0.0038 | 0.0005 | 0.0047 | 0 | 0.0047 | 0.0024 | 0.0317 | 0.0004 | 0.001 | 0.0002 | 0.0317 | 0.0002 | 0 | 0 |
Bud | 0.0007 | 0.0014 | 0.0019 | 0.0007 | 0.0013 | 0.0004 | 0.0004 | 0.0011 | 0.002 | 0.0009 | 0.0006 | 0.0005 | 0.0003 | 0.0007 | 0.0012 | 0.0005 | 0.0002 | 0.0003 |
Bud Neck | 0.0078 | 0.0023 | 0.0014 | 0.0007 | 0.0018 | 0.0518 | 0.0021 | 0.0035 | 0.0016 | 0.0008 | 0.0021 | 0.0539 | 0.0006 | 0.002 | 0.0016 | 0.0014 | 0.0147 | 0.0673 |
Bud Periphery | 0.0015 | 0.0024 | 0.003 | 0.0018 | 0.0041 | 0.0015 | 0.0013 | 0.0026 | 0.0045 | 0.0024 | 0.0015 | 0.0017 | 0.0007 | 0.0011 | 0.002 | 0.0011 | 0.0002 | 0.0009 |
Bud Site | 0.004 | 0.0065 | 0.0019 | 0.0012 | 0.0024 | 0.0007 | 0.0022 | 0.0077 | 0.0043 | 0.0008 | 0.0016 | 0.0009 | 0.0008 | 0.0061 | 0.0051 | 0.0022 | 0.0004 | 0.0006 |
Cell Periphery | 0.0019 | 0.0026 | 0.0018 | 0.001 | 0.0012 | 0.0023 | 0.0016 | 0.0028 | 0.0029 | 0.0016 | 0.0014 | 0.0018 | 0.0028 | 0.0032 | 0.0014 | 0.0008 | 0.0002 | 0.0018 |
Cytoplasm | 0.0083 | 0.0189 | 0.0177 | 0.011 | 0.0118 | 0.0048 | 0.0053 | 0.0176 | 0.0192 | 0.0283 | 0.0241 | 0.0032 | 0.0138 | 0.006 | 0.0135 | 0.0136 | 0.0266 | 0.0034 |
Cytoplasmic Foci | 0.0477 | 0.0227 | 0.0362 | 0.0158 | 0.0244 | 0.0234 | 0.0426 | 0.0119 | 0.0218 | 0.0645 | 0.0384 | 0.023 | 0.0174 | 0.0156 | 0.0328 | 0.0215 | 0.0464 | 0.0254 |
Eisosomes | 0.0004 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0.0003 | 0.0002 | 0 | 0 | 0.0004 | 0.0003 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0083 | 0.0042 | 0.004 | 0.0035 | 0.0081 | 0.0037 | 0.0041 | 0.0029 | 0.0053 | 0.0032 | 0.003 | 0.0034 | 0.0019 | 0.0027 | 0.0048 | 0.0104 | 0.0028 | 0.0015 |
Endosome | 0.2502 | 0.1545 | 0.2337 | 0.3473 | 0.3 | 0.2702 | 0.2688 | 0.1493 | 0.2284 | 0.1696 | 0.2296 | 0.2496 | 0.1867 | 0.1193 | 0.194 | 0.2215 | 0.4323 | 0.1213 |
Golgi | 0.0204 | 0.0034 | 0.0157 | 0.0149 | 0.0176 | 0.017 | 0.0117 | 0.0039 | 0.0086 | 0.0054 | 0.0219 | 0.018 | 0.0055 | 0.0059 | 0.0093 | 0.0136 | 0.0199 | 0.0112 |
Lipid Particles | 0.0412 | 0.0118 | 0.0339 | 0.0144 | 0.074 | 0.0322 | 0.0523 | 0.0181 | 0.0278 | 0.0185 | 0.043 | 0.0247 | 0.0205 | 0.0246 | 0.0156 | 0.0762 | 0.0062 | 0.0329 |
Mitochondria | 0.0445 | 0.0256 | 0.0742 | 0.0523 | 0.0601 | 0.0145 | 0.0506 | 0.0092 | 0.0477 | 0.0065 | 0.0157 | 0.0102 | 0.0153 | 0.0167 | 0.0113 | 0.0091 | 0.0008 | 0.0033 |
None | 0.0415 | 0.0303 | 0.0358 | 0.002 | 0.0016 | 0.0004 | 0.0161 | 0.0358 | 0.0357 | 0.0145 | 0.0038 | 0.0002 | 0.0048 | 0.0267 | 0.0368 | 0.0006 | 0.0015 | 0.0001 |
Nuclear Periphery | 0.0195 | 0.0177 | 0.0066 | 0.0115 | 0.0178 | 0.023 | 0.0098 | 0.0048 | 0.0064 | 0.0185 | 0.0039 | 0.0048 | 0.0052 | 0.0067 | 0.0056 | 0.0089 | 0.004 | 0.0028 |
Nucleolus | 0.0038 | 0.0099 | 0.0054 | 0.0024 | 0.0029 | 0.0022 | 0.005 | 0.0083 | 0.0083 | 0.0038 | 0.0035 | 0.0022 | 0.0126 | 0.0069 | 0.0057 | 0.0045 | 0.0015 | 0.0021 |
Nucleus | 0.0075 | 0.0106 | 0.0064 | 0.0059 | 0.0056 | 0.0052 | 0.0045 | 0.0067 | 0.0082 | 0.0197 | 0.0054 | 0.0029 | 0.009 | 0.0058 | 0.015 | 0.0028 | 0.0021 | 0.0021 |
Peroxisomes | 0.0141 | 0.0029 | 0.0112 | 0.0023 | 0.0132 | 0.0039 | 0.0071 | 0.0014 | 0.0137 | 0.0085 | 0.0205 | 0.0062 | 0.0053 | 0.001 | 0.0088 | 0.0325 | 0.0043 | 0.0022 |
Punctate Nuclear | 0.0044 | 0.0008 | 0.0045 | 0.0003 | 0.0022 | 0.0005 | 0.0123 | 0.0007 | 0.0011 | 0.0072 | 0.0053 | 0.0004 | 0.0034 | 0.0004 | 0.0031 | 0.0013 | 0.0004 | 0.0002 |
Vacuole | 0.3397 | 0.5305 | 0.3733 | 0.4064 | 0.3634 | 0.4178 | 0.3711 | 0.5757 | 0.4401 | 0.4706 | 0.4712 | 0.4769 | 0.5361 | 0.5901 | 0.4845 | 0.4814 | 0.3652 | 0.5713 |
Vacuole Periphery | 0.1067 | 0.1408 | 0.124 | 0.1046 | 0.0825 | 0.124 | 0.1263 | 0.136 | 0.1077 | 0.1518 | 0.0716 | 0.1153 | 0.1562 | 0.158 | 0.1159 | 0.096 | 0.0705 | 0.1493 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 8.7019 | 19.7068 | 52.336 | 51.5982 | 13.5389 | 4.6385 | 35.9183 | 59.8099 | 45.8144 | 18.4438 |
Translational Efficiency | 0.4626 | 1.5851 | 1.7234 | 1.0338 | 0.9576 | 0.9467 | 1.5037 | 1.2427 | 1.6246 | 1.3614 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1575 | 989 | 185 | 1531 | 1619 | 1591 | 202 | 1219 | 3194 | 2580 | 387 | 2750 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 762.66 | 962.15 | 946.07 | 910.24 | 733.40 | 848.45 | 966.87 | 948.17 | 747.83 | 892.03 | 956.93 | 927.05 |
Standard Deviation | 135.85 | 170.57 | 93.61 | 155.16 | 114.70 | 141.59 | 113.21 | 167.93 | 126.43 | 163.01 | 104.82 | 162.04 |
Intensity Change Log 2 | — | 0.335222 | 0.310907 | 0.255207 | — | 0.210229 | 0.398722 | 0.370545 | — | 0.275300 | 0.354624 | 0.312901 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000029 | 0.000066 | 0.001031 | 0.000527 | 0.000042 | 0.000061 | 0.000426 | 0.000288 | 0.000035 | 0.000063 | 0.000715 | 0.000421 |
Bud Neck | 0.016962 | 0.006489 | 0.019731 | 0.017182 | 0.021770 | 0.008452 | 0.043138 | 0.022523 | 0.019399 | 0.007699 | 0.031949 | 0.019549 |
Bud Site | 0.019443 | 0.010684 | 0.031468 | 0.046984 | 0.021483 | 0.011403 | 0.045414 | 0.054063 | 0.020477 | 0.011128 | 0.038747 | 0.050122 |
Cell Periphery | 0.001385 | 0.001740 | 0.000217 | 0.000221 | 0.000899 | 0.001721 | 0.000179 | 0.000245 | 0.001139 | 0.001728 | 0.000197 | 0.000231 |
Cytoplasm | 0.042822 | 0.010646 | 0.139207* | 0.081583 | 0.087235 | 0.013973 | 0.177705* | 0.112885* | 0.065335 | 0.012697 | 0.159302* | 0.095458 |
Cytoplasmic Foci | 0.018465 | 0.010633 | 0.064481 | 0.055074 | 0.031455 | 0.010596 | 0.060468 | 0.045146 | 0.025049 | 0.010610 | 0.062386 | 0.050673 |
Eisosomes | 0.000030 | 0.000031 | 0.000112 | 0.000026 | 0.000027 | 0.000021 | 0.000086 | 0.000028 | 0.000028 | 0.000025 | 0.000098 | 0.000027 |
Endoplasmic Reticulum | 0.002763 | 0.003224 | 0.009419 | 0.004252 | 0.001690 | 0.003142 | 0.004824 | 0.002976 | 0.002219 | 0.003173 | 0.007020 | 0.003686 |
Endosome | 0.096650 | 0.065317 | 0.125143 | 0.199167 | 0.088225 | 0.066695 | 0.097960 | 0.150555 | 0.092379 | 0.066166 | 0.110954 | 0.177619 |
Golgi | 0.002919 | 0.003594 | 0.007231 | 0.017250 | 0.002349 | 0.003275 | 0.003218 | 0.010477 | 0.002630 | 0.003397 | 0.005136 | 0.014248 |
Lipid Particles | 0.002198 | 0.000901 | 0.007310 | 0.004487 | 0.001601 | 0.001171 | 0.012001 | 0.003690 | 0.001896 | 0.001067 | 0.009758 | 0.004134 |
Mitochondria | 0.001851 | 0.002528 | 0.000650 | 0.002141 | 0.003208 | 0.002826 | 0.000645 | 0.001764 | 0.002539 | 0.002711 | 0.000647 | 0.001974 |
Mitotic Spindle | 0.000182 | 0.000499 | 0.040993 | 0.015106 | 0.000496 | 0.001301 | 0.008512 | 0.024274 | 0.000341 | 0.000993 | 0.024039 | 0.019170 |
None | 0.001845 | 0.000160 | 0.000843 | 0.001147 | 0.000823 | 0.000184 | 0.000915 | 0.000514 | 0.001327 | 0.000175 | 0.000881 | 0.000866 |
Nuclear Periphery | 0.000196 | 0.000248 | 0.003122 | 0.001414 | 0.000142 | 0.000168 | 0.001758 | 0.001941 | 0.000169 | 0.000199 | 0.002410 | 0.001648 |
Nuclear Periphery Foci | 0.000268 | 0.000586 | 0.013103 | 0.004958 | 0.000158 | 0.000265 | 0.007204 | 0.003293 | 0.000212 | 0.000388 | 0.010024 | 0.004220 |
Nucleolus | 0.001730 | 0.001474 | 0.001620 | 0.002178 | 0.000539 | 0.000926 | 0.001769 | 0.002805 | 0.001127 | 0.001136 | 0.001698 | 0.002456 |
Nucleus | 0.012147 | 0.006844 | 0.295639* | 0.016568 | 0.010855 | 0.003770 | 0.156912* | 0.026160 | 0.011492 | 0.004948 | 0.223229* | 0.020820 |
Peroxisomes | 0.000901 | 0.001108 | 0.016484 | 0.004444 | 0.001504 | 0.001751 | 0.012463 | 0.006164 | 0.001207 | 0.001504 | 0.014385 | 0.005206 |
Vacuole | 0.732587* | 0.779370* | 0.220288* | 0.500013* | 0.696368* | 0.823980* | 0.361098* | 0.505431* | 0.714228* | 0.806880* | 0.293786* | 0.502415* |
Vacuole Periphery | 0.044629 | 0.093857 | 0.001908 | 0.025278 | 0.029132 | 0.044322 | 0.003304 | 0.024778 | 0.036774 | 0.063310 | 0.002637 | 0.025057 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.33 | -5.41 | -5.43 | -4.87 | 2.04 | -1.12 | -6.65 | -4.95 | -4.40 | 1.83 | -2.37 | -7.27 | -6.86 | -6.21 | 2.29 |
Bud Neck | 6.45 | -0.21 | 0.05 | -7.48 | 0.24 | 7.58 | -0.70 | 0.08 | -6.80 | 0.73 | 9.71 | -0.72 | 0.30 | -9.71 | 0.84 |
Bud Site | 3.95 | -0.40 | -7.30 | -9.86 | -3.79 | 4.89 | -0.60 | -6.60 | -9.62 | -3.05 | 6.28 | -0.73 | -9.79 | -13.87 | -4.70 |
Cell Periphery | -1.46 | 7.54 | 7.54 | 7.59 | 0.34 | -4.32 | 6.83 | 5.98 | 9.23 | -1.01 | -3.83 | 10.10 | 9.65 | 12.03 | -0.68 |
Cytoplasm | 8.85 | -8.43 | -9.87 | -17.37 | 3.66 | 14.64 | -7.31 | -7.24 | -19.60 | 3.21 | 16.80 | -10.92 | -11.14 | -25.93 | 5.13 |
Cytoplasmic Foci | 3.31 | -4.46 | -12.38 | -14.42 | -1.15 | 8.28 | -1.81 | -2.89 | -12.14 | 0.27 | 8.55 | -4.43 | -10.50 | -19.43 | -0.83 |
Eisosomes | -0.46 | -9.08 | 0.92 | 1.41 | 9.72 | 2.19 | -8.57 | -1.02 | -3.78 | 8.37 | 1.17 | -12.36 | 0.20 | -1.29 | 12.78 |
Endoplasmic Reticulum | -1.49 | -6.59 | -1.55 | -0.95 | 4.89 | -5.63 | -6.99 | -2.96 | 1.35 | 4.83 | -4.64 | -8.81 | -2.56 | -0.45 | 6.06 |
Endosome | 4.95 | 1.59 | -11.41 | -15.54 | -7.42 | 3.97 | 3.55 | -5.28 | -8.73 | -6.79 | 6.39 | 3.44 | -12.41 | -17.97 | -10.49 |
Golgi | -0.76 | -0.54 | -8.25 | -7.11 | -5.02 | -1.31 | 0.86 | -5.28 | -3.97 | -4.96 | -1.38 | -0.07 | -9.84 | -8.26 | -7.16 |
Lipid Particles | 2.28 | -6.05 | -3.30 | -5.97 | 2.95 | 1.31 | -2.73 | -2.44 | -2.95 | 1.93 | 2.55 | -4.30 | -4.27 | -6.22 | 2.63 |
Mitochondria | -1.17 | 4.61 | -0.50 | 0.90 | -5.86 | 0.39 | 4.22 | 2.37 | 1.61 | -4.24 | -0.30 | 5.64 | 1.84 | 1.70 | -7.23 |
Mitotic Spindle | -1.59 | -2.94 | -8.20 | -7.91 | 0.94 | -1.52 | -2.41 | -7.57 | -7.27 | -3.31 | -2.08 | -3.70 | -10.92 | -10.41 | -0.87 |
None | 2.97 | 1.74 | 1.18 | -2.68 | -0.63 | 4.06 | -1.11 | 0.77 | -4.63 | 1.82 | 3.96 | 1.08 | 1.24 | -3.73 | 0.39 |
Nuclear Periphery | -0.96 | -8.08 | -6.75 | -6.34 | 4.83 | -0.73 | -11.02 | -5.44 | -5.37 | 1.01 | -0.98 | -12.08 | -8.33 | -8.14 | 4.51 |
Nuclear Periphery Foci | -1.17 | -3.21 | -5.84 | -4.99 | 1.71 | -1.31 | -7.31 | -4.38 | -4.22 | 3.05 | -1.47 | -4.99 | -7.29 | -6.74 | 2.48 |
Nucleolus | 0.26 | 0.19 | -0.59 | -1.06 | -1.09 | -2.01 | -2.63 | -4.75 | -3.81 | -2.29 | -0.12 | -1.26 | -3.65 | -3.97 | -2.45 |
Nucleus | 2.47 | -14.63 | -3.34 | -6.07 | 14.30 | 6.18 | -11.22 | -8.89 | -12.29 | 9.71 | 5.76 | -17.83 | -8.72 | -13.94 | 16.76 |
Peroxisomes | -0.94 | -2.59 | -8.29 | -6.65 | 1.61 | -0.64 | -2.04 | -6.03 | -5.02 | 0.53 | -1.22 | -3.31 | -9.72 | -7.52 | 1.63 |
Vacuole | -4.32 | 23.85 | 20.34 | 23.85 | -11.87 | -12.90 | 12.99 | 15.48 | 28.56 | -4.28 | -12.81 | 24.44 | 25.20 | 38.28 | -10.49 |
Vacuole Periphery | -9.89 | 21.16 | 8.94 | 14.98 | -15.33 | -5.82 | 16.16 | 3.74 | 8.72 | -9.52 | -10.35 | 26.41 | 8.94 | 16.80 | -17.19 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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