Standard name
Human Ortholog
Description Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p; required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 – 0.05 0.06 0.05 0.06 0 0 0 0 0 0.05 0.14 0.15 0.16 0.05 0.05 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 – 0 0 0 0.13 0.13 0.18 0.3 0.31 0.27 0.33 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.98 – 0.91 0.91 0.89 0.86 0.79 0.81 0.73 0.77 0.79 0.76 0.83 0.8 0.78 0.92 0.89 0.88 0.95 0.99 0.91 0.8 0.69 0.59
Nuclear Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 – 0 0 0 0.06 0.11 0.11 0.11 0.13 0.15 0.14 0 0 0 0 0 0 0 0 0 0 0.06 0.09
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.2 0.25
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 – 0 0 1 0 1 3 0 1 1 3 0 0 0 0 0 0 0 0 0 0 1 2
Bud Neck 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 1 0 1 1 2 7 13 8 24 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 – 11 13 19 16 13 12 11 15 8 19 53 72 81 29 24 30 0 0 0 0 0 1
Endoplasmic Reticulum 0 – 1 0 0 1 2 0 1 0 0 0 0 0 0 10 6 7 1 0 2 0 1 0
Endosome 0 – 0 0 0 0 0 0 0 0 0 0 1 5 8 1 0 0 1 0 2 3 1 2
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 3 2 2
Mitochondria 6 – 3 9 1 36 40 57 95 107 105 133 0 0 3 3 2 4 0 0 0 1 2 2
Nucleus 217 – 220 193 318 246 247 260 230 267 302 311 318 379 391 591 390 358 210 25 231 172 204 202
Nuclear Periphery 1 – 0 3 3 0 6 3 7 3 5 7 0 5 6 1 0 3 0 0 0 0 2 4
Nucleolus 3 – 7 7 16 17 35 36 35 45 58 56 1 10 7 5 7 10 0 0 5 7 17 31
Peroxisomes 0 – 0 0 1 0 0 0 0 0 0 0 6 9 13 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 1 0 0 0 0 0 0 1 8 11 14 1 0 0 0 0 0 0 1 0
Vac/Vac Membrane 1 – 6 4 8 3 4 5 4 7 3 14 2 5 8 27 19 7 4 0 8 23 57 85
Unique Cell Count 221 241 212 356 285 312 320 313 347 384 407 382 474 502 642 438 407 222 26 255 215 295 342
Labelled Cell Count 230 248 230 369 320 349 378 390 458 490 568 389 496 531 668 448 420 222 26 255 215 295 342


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.8 – 9.7 8.3 8.4 6.7 6.1 6.1 5.8 5.5 5.4 5.3 10.1 9.5 9.3 14.5 16.2 15.9 9.4 9.5 10.1
Std Deviation (1e-4) 1.7 – 3.5 2.8 2.9 2.0 1.8 1.8 2.3 1.9 1.6 2.1 3.1 3.1 2.8 4.2 5.4 4.6 1.8 1.8 1.7
Intensity Change (Log2) – – – -0.24 -0.21 -0.53 -0.68 -0.67 -0.75 -0.84 -0.86 -0.87 0.06 -0.03 -0.07 0.58 0.73 0.71 -0.05 -0.04 0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 3.0 0 3.8 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.7 0.4 0.5 -0.2 -0.5 -0.6 -0.1 -1.8 0.1 3.7 4.2 4.5 0 0.5 1.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.0 0 5.0 5.0 6.3 8.9 9.0 8.4 9.5 0 0 0 0 0 0
Nucleus -0.1 -0.8 -1.8 -3.9 -3.3 -5.3 -4.5 -4.1 -4.8 -2.8 -3.9 -4.5 0.4 -0.9 -1.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.2 1.0 1.7 3.7 3.7 3.6 4.2 4.9 4.5 0 -0.7 0 0 0 -0.3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 -0.2 0 0 0 0 -0.4 0 0.7 0 0 0 1.2 1.2 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.1743 8.559 7.6045 7.5746 7.2273 7.5677 10.0893 10.2324 9.2563 8.6762 8.2555 9.1605 11.4284 10.7355 10.8232 10.8524 10.3127 10.8308
Actin 0.0027 0 0.0032 0 0 0.001 0.0023 0 0.0227 0 0.0001 0.0026 0.0037 0 0.0095 0.0002 0.0011 0.0001
Bud 0.0004 0 0 0 0 0 0.001 0 0.0004 0 0.0003 0.0001 0.0009 0 0 0 0.0005 0
Bud Neck 0.0006 0.0001 0.0001 0.0001 0.0005 0.0007 0.0013 0 0.0004 0 0.0002 0.0004 0.0006 0 0.0001 0.0001 0.0016 0.0002
Bud Periphery 0.0007 0 0 0 0 0.0001 0.0037 0 0.001 0 0.0015 0.0004 0.0024 0 0.0001 0 0.0022 0
Bud Site 0.0027 0.0001 0 0 0 0 0.0013 0 0.0005 0 0.0003 0.0003 0.0053 0 0.0006 0 0.0004 0
Cell Periphery 0.0002 0 0 0 0 0.0001 0.0013 0.0001 0.0004 0 0.0008 0.0003 0.001 0 0.0001 0 0.0007 0
Cytoplasm 0.0008 0.0026 0 0.0011 0 0 0.0026 0.0001 0.0016 0 0.0002 0.0016 0.0017 0 0.0003 0.0004 0.0004 0.0012
Cytoplasmic Foci 0.0024 0 0.0004 0.0042 0 0.0001 0.0026 0 0.0008 0 0.0001 0.0027 0.0136 0 0.0005 0 0.0039 0
Eisosomes 0.0001 0 0 0 0 0 0.0001 0 0.0002 0 0 0.0001 0.0001 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0006 0 0 0.0006 0 0.0003 0.0033 0 0.0026 0 0.0004 0.0026 0.0007 0 0.0001 0 0.0007 0
Endosome 0.0023 0 0.0001 0.002 0 0.0006 0.0027 0 0.0041 0 0.001 0.0072 0.0046 0 0.0004 0 0.0259 0
Golgi 0.0006 0 0.0001 0.0001 0 0.0003 0.0005 0 0.007 0 0.0001 0.001 0.0037 0 0.0003 0.0001 0.01 0
Lipid Particles 0.0018 0 0.0002 0.0028 0 0.0006 0.0082 0 0.0017 0 0.0021 0.0114 0.0092 0 0.0009 0 0.0254 0
Mitochondria 0.0015 0.0001 0.0001 0.0002 0.0002 0.0003 0.0075 0.0001 0.0089 0 0.0125 0.0083 0.0166 0.0001 0.0013 0.083 0.027 0.0009
None 0.001 0 0 0.0024 0 0.0002 0.0004 0 0.0003 0 0.0003 0.0004 0.0006 0 0.0001 0 0.0003 0.007
Nuclear Periphery 0.0023 0.002 0.0022 0.0043 0.001 0.001 0.0164 0.0049 0.0075 0.0002 0.0108 0.01 0.0021 0.0004 0.0014 0.0018 0.0029 0.0003
Nucleolus 0.0194 0.0065 0.0057 0.0193 0.0885 0.0535 0.0167 0.0047 0.0055 0.0022 0.1219 0.0297 0.0159 0.0144 0.0051 0.0084 0.0731 0.027
Nucleus 0.9512 0.9866 0.9805 0.9563 0.9061 0.9408 0.9026 0.9817 0.9168 0.9975 0.8118 0.9095 0.8961 0.9848 0.975 0.9052 0.7912 0.9629
Peroxisomes 0.0005 0 0.0025 0.0001 0 0 0.0004 0 0.0002 0 0.0001 0.0005 0.0108 0 0.0001 0.0001 0.0031 0
Punctate Nuclear 0.0061 0.0001 0.005 0.0049 0.0032 0.0003 0.0157 0.0001 0.0007 0.0001 0.0004 0.008 0.0034 0.0003 0.0001 0.0003 0.0011 0.0001
Vacuole 0.0012 0.0015 0 0.0008 0.0001 0.0002 0.0065 0.0036 0.0108 0 0.0124 0.0017 0.0046 0 0.0016 0.0001 0.0166 0.0001
Vacuole Periphery 0.001 0.0003 0 0.0005 0.0001 0.0001 0.0031 0.0047 0.0056 0 0.0226 0.0013 0.0022 0 0.0021 0.0002 0.0118 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 46.839 48.7354 28.5235 35.9066 54.2577 32.9994 40.2539 27.4029 27.5078 41.8435
Translational Efficiency 1.4762 1.3567 1.7747 1.1667 0.8839 1.1668 1.1808 1.0894 0.9599 1.0397

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
118 1238 1890 1525 1493 1678 745 9 1611 2916 2635 1534

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 799.36 964.33 1159.15 1436.80 858.96 988.53 1308.11 1387.80 854.59 978.26 1201.27 1436.51
Standard Deviation 104.87 223.79 195.12 374.44 139.62 174.18 204.13 330.01 138.25 197.14 208.78 374.21
Intensity Change Log 2 — 0.270682 0.536150 0.845942 — 0.202694 0.606821 0.692137 — 0.235866 0.573187 0.768324

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000286 0.000748 0.000478 0.000526 0.000310 0.000536 0.000529 0.001691 0.000308 0.000626 0.000492 0.000533
Bud Neck 0.003137 0.007579 0.012164 0.017827 0.003653 0.008933 0.011648 0.005457 0.003615 0.008358 0.012018 0.017754
Bud Site 0.003401 0.001354 0.000763 0.001160 0.000356 0.001723 0.000684 0.001829 0.000579 0.001566 0.000741 0.001164
Cell Periphery 0.000046 0.000515 0.000115 0.000138 0.000105 0.000323 0.000066 0.000905 0.000101 0.000405 0.000102 0.000143
Cytoplasm 0.001285 0.004863 0.000610 0.000885 0.000373 0.001022 0.000138 0.002120 0.000440 0.002653 0.000476 0.000893
Cytoplasmic Foci 0.000390 0.000586 0.000012 0.000033 0.000013 0.000529 0.000011 0.000046 0.000041 0.000554 0.000012 0.000033
Eisosomes 0.000021 0.000056 0.000068 0.000039 0.000037 0.000065 0.000062 0.000072 0.000035 0.000061 0.000066 0.000040
Endoplasmic Reticulum 0.002552 0.006684 0.001931 0.001186 0.004595 0.007241 0.001375 0.002586 0.004445 0.007005 0.001774 0.001194
Endosome 0.000643 0.000386 0.000045 0.000091 0.000124 0.000954 0.000042 0.000434 0.000162 0.000713 0.000044 0.000093
Golgi 0.000096 0.000211 0.000028 0.000049 0.000030 0.000587 0.000024 0.000119 0.000035 0.000427 0.000027 0.000050
Lipid Particles 0.000192 0.000262 0.000011 0.000013 0.000022 0.000437 0.000008 0.000040 0.000034 0.000363 0.000010 0.000013
Mitochondria 0.000688 0.001722 0.000874 0.001869 0.000448 0.002144 0.001188 0.003143 0.000465 0.001965 0.000962 0.001876
Mitotic Spindle 0.001889 0.001017 0.000891 0.001366 0.000056 0.003623 0.000045 0.007255 0.000191 0.002517 0.000652 0.001401
None 0.003398 0.007759 0.001292 0.001776 0.002588 0.002299 0.000837 0.008792 0.002647 0.004617 0.001164 0.001817
Nuclear Periphery 0.000125 0.000250 0.000130 0.000664 0.000113 0.000586 0.000204 0.000878 0.000114 0.000443 0.000151 0.000665
Nuclear Periphery Foci 0.000090 0.000288 0.000015 0.000224 0.000074 0.000572 0.000016 0.000119 0.000075 0.000452 0.000015 0.000223
Nucleolus 0.038053 0.009859 0.007123 0.012823 0.013073 0.007467 0.005850 0.005818 0.014902 0.008483 0.006763 0.012782
Nucleus 0.941572* 0.953385* 0.972856* 0.958238* 0.973205* 0.957731* 0.976485* 0.957229* 0.970888* 0.955886* 0.973882* 0.958232*
Peroxisomes 0.000369 0.000479 0.000212 0.000203 0.000205 0.000308 0.000305 0.000451 0.000217 0.000380 0.000239 0.000204
Vacuole 0.001006 0.001698 0.000312 0.000617 0.000454 0.002113 0.000382 0.000870 0.000494 0.001937 0.000332 0.000619
Vacuole Periphery 0.000763 0.000296 0.000070 0.000272 0.000168 0.000806 0.000100 0.000145 0.000211 0.000590 0.000078 0.000271

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.84 -0.59 -1.25 1.24 -2.24 NaN NaN NaN NaN NaN -5.29 -10.93 -6.72 0.50 -1.76
Bud Neck -2.60 -6.64 -9.88 -9.71 -6.35 NaN NaN NaN NaN NaN -7.69 -14.29 -13.71 -9.55 -6.18
Bud Site 0.65 0.84 0.64 -0.09 -2.48 NaN NaN NaN NaN NaN -2.85 -1.06 -2.59 0.48 -2.49
Cell Periphery -3.87 -2.45 -1.87 2.92 -0.50 NaN NaN NaN NaN NaN -5.08 0.08 -0.86 3.49 -0.87
Cytoplasm -2.47 1.20 0.52 3.09 -1.54 NaN NaN NaN NaN NaN -3.97 -0.00 -2.21 2.75 -2.14
Cytoplasmic Foci -0.17 1.77 1.65 2.04 -2.20 NaN NaN NaN NaN NaN -3.47 1.71 0.20 3.52 -2.22
Eisosomes -6.96 -7.58 -4.90 3.66 4.90 NaN NaN NaN NaN NaN -6.02 -6.32 -1.09 5.71 6.01
Endoplasmic Reticulum -6.51 1.88 3.68 12.78 8.30 NaN NaN NaN NaN NaN -7.09 13.38 15.89 18.43 7.27
Endosome 0.49 1.18 1.04 2.55 -1.19 NaN NaN NaN NaN NaN -3.21 2.36 0.66 3.45 -1.24
Golgi -1.36 1.61 0.78 2.12 -3.54 NaN NaN NaN NaN NaN -2.68 0.40 -2.79 2.44 -3.61
Lipid Particles -0.21 1.63 1.56 1.84 -1.46 NaN NaN NaN NaN NaN -2.23 2.36 1.32 2.33 -1.48
Mitochondria -1.09 0.35 -2.13 -0.95 -7.54 NaN NaN NaN NaN NaN -3.76 -8.36 -10.21 -0.33 -6.84
Mitotic Spindle 0.53 0.26 0.06 -1.09 -0.40 NaN NaN NaN NaN NaN -3.34 -1.72 -2.74 0.61 -1.04
None -2.20 2.51 1.98 3.94 -1.08 NaN NaN NaN NaN NaN -2.35 3.36 1.70 3.61 -1.40
Nuclear Periphery 0.22 1.05 -2.07 -2.54 -3.06 NaN NaN NaN NaN NaN -3.70 -1.45 -3.21 -1.43 -2.97
Nuclear Periphery Foci -1.77 1.93 -0.83 0.53 -2.00 NaN NaN NaN NaN NaN -3.12 2.19 -1.43 1.42 -2.02
Nucleolus 4.19 4.47 2.99 -4.12 -5.36 NaN NaN NaN NaN NaN 6.04 6.91 -1.52 -5.14 -5.64
Nucleus -1.33 -2.90 -0.94 1.08 7.06 NaN NaN NaN NaN NaN 5.63 -0.36 6.89 1.93 7.48
Peroxisomes -0.45 1.93 1.24 1.48 -1.47 NaN NaN NaN NaN NaN -2.50 -2.11 -1.91 1.34 -0.78
Vacuole -0.79 0.85 0.28 3.16 -3.26 NaN NaN NaN NaN NaN -5.16 0.71 -1.29 4.43 -2.89
Vacuole Periphery 0.71 1.07 0.70 -0.09 -4.21 NaN NaN NaN NaN NaN -2.54 1.76 -0.97 2.06 -4.02
Standard name
Human Ortholog
Description Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p; required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Gcd10

Gcd10


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gcd10-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available