Standard name
Human Ortholog
Description Serine-rich protein of unknown function; predicted to be palmitoylated; overproduction suppresses growth inhibition caused by exposure to immunosuppressant leflunomide; MLF3 has a paralog, VHS2, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0.05 0.05 0.05 0 0 0 0 0 0 0 0.05 0 0 0 0.06
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.15 0.19 0.34 0.34 0.22 0.26 0.19 0.22 0.28 0.24 0.24 0.31 0.18 0.2 0.17 0.21 0.2 0.28 0.1 0 0.21 0.06 0.06 0.1
Cytoplasm 0.92 0.99 0.95 0.92 0.88 0.84 0.83 0.76 0.7 0.73 0.69 0.66 1.0 0.98 0.99 0.94 0.97 0.97 0.76 0.85 0.61 0.86 0.84 0.78
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.12 0 0 0 0.07 0.19 0.26 0.28 0.29 0.29 0.36 0.33 0 0 0 0 0 0 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 0 0 1 3 2 7 4 5 0 0 0 0 0 0 0 1 4 4 1 2 3
Bud 5 6 2 0 3 5 9 15 9 23 11 14 0 0 0 1 1 0 3 8 6 0 10 15
Bud Neck 1 0 1 0 1 0 0 2 1 0 0 1 0 0 0 0 0 0 1 1 2 0 2 3
Bud Site 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Cell Periphery 28 36 95 50 43 72 70 94 94 108 58 81 52 46 57 93 67 91 19 6 58 10 18 26
Cytoplasm 177 183 263 135 169 229 300 330 232 327 167 172 282 227 341 406 318 311 150 155 169 134 249 211
Endoplasmic Reticulum 0 0 3 4 1 4 1 6 3 2 3 4 1 0 3 11 4 6 0 0 6 1 0 3
Endosome 0 0 0 1 0 0 1 0 0 0 0 0 1 0 0 0 0 1 1 0 3 0 1 1
Golgi 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 1
Mitochondria 24 1 2 5 14 52 93 122 95 131 87 85 0 0 0 3 3 1 7 1 15 0 0 1
Nucleus 0 0 1 0 1 1 2 3 1 0 1 1 0 1 1 6 4 3 0 0 0 0 3 0
Nuclear Periphery 0 0 0 0 0 1 1 4 1 2 0 1 0 0 0 0 0 1 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 2 1 0 3 2 1 0 0 0 0 1 0 0 0 5 1 4 0 2 1
Vac/Vac Membrane 0 0 2 0 0 1 4 8 4 9 2 1 0 2 1 20 7 5 2 1 1 0 1 0
Unique Cell Count 192 185 278 146 192 273 361 435 330 447 242 260 283 231 345 433 327 322 197 183 277 157 299 272
Labelled Cell Count 235 227 370 196 235 367 484 590 449 607 334 360 336 276 404 541 404 419 197 183 277 157 299 272


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 6.5 6.5 6.1 5.6 5.1 5.2 5.3 5.3 5.3 5.0 5.5 8.1 7.8 7.4 9.7 9.0 9.2 7.7 7.8 8.5
Std Deviation (1e-4) 1.8 2.4 2.2 2.5 2.2 2.1 2.2 1.7 1.7 1.6 1.8 1.6 2.1 2.1 2.2 1.4 1.5 1.4 2.1 2.1 2.3
Intensity Change (Log2) -0.1 -0.22 -0.34 -0.32 -0.28 -0.29 -0.28 -0.36 -0.23 0.33 0.26 0.2 0.58 0.47 0.5 0.25 0.28 0.39


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 2.3 1.9 3.2 2.8 3.2 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 -2.8 -2.0 -4.2 -3.7 -1.5 -2.9 -2.5 -0.7 -4.3 -3.6 -5.1 -3.7 -3.8 -1.6
Cytoplasm -0.9 -2.6 -4.1 -4.5 -6.5 -7.7 -7.2 -7.7 -8.4 3.6 2.2 3.1 -0.5 1.7 1.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 1.4 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 3.9 7.2 8.8 9.4 9.4 9.7 10.6 10.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 2.9 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.0931 2.1751 2.3224 2.4252 2.2483 1.9016 1.7283 1.3037 1.6359 0.5085 1.724 1.6753 0.8321 0.8889 0.73 1.3223 1.8333 0.7933
Actin 0.0165 0.0021 0.0023 0.0443 0.0015 0.0058 0.0261 0.0027 0.0088 0.002 0.0058 0.0117 0.012 0.0018 0.0084 0.0043 0.0772 0.0141
Bud 0.0011 0.0012 0.0008 0.0156 0.0062 0.0008 0.004 0.006 0.0027 0.0012 0.0044 0.0011 0.0011 0.0022 0.0033 0.0005 0.0034 0.0018
Bud Neck 0.0006 0.0005 0.0011 0.0011 0.0009 0.0028 0.0123 0.0019 0.0022 0.0008 0.0027 0.0071 0.0086 0.0008 0.0056 0.0007 0.0086 0.0079
Bud Periphery 0.0013 0.0047 0.0015 0.0107 0.0396 0.0008 0.009 0.0067 0.0085 0.0023 0.0237 0.0028 0.0021 0.0057 0.0075 0.0008 0.0059 0.0046
Bud Site 0.0094 0.0063 0.0054 0.0043 0.0005 0.0002 0.0193 0.0271 0.0094 0.0022 0.003 0.0013 0.0108 0.0442 0.014 0.0003 0.0209 0.0009
Cell Periphery 0.0122 0.0039 0.0107 0.0033 0.0048 0.0022 0.0032 0.0016 0.0021 0.0007 0.0024 0.0006 0.0033 0.0034 0.0057 0.0006 0.0014 0.0013
Cytoplasm 0.4779 0.5183 0.5223 0.5924 0.6952 0.5997 0.4415 0.453 0.5025 0.322 0.4945 0.5505 0.4929 0.445 0.5035 0.4214 0.547 0.5062
Cytoplasmic Foci 0.0168 0.0028 0.0029 0.0047 0.0019 0.0028 0.013 0.0042 0.0081 0.0229 0.0041 0.0107 0.012 0.0038 0.0051 0.0151 0.0061 0.005
Eisosomes 0.0007 0.0001 0.0004 0.0007 0.0001 0.0001 0.0003 0.0002 0.0001 0.0001 0.0001 0.0001 0.001 0.0001 0.0002 0.0001 0.0006 0.0001
Endoplasmic Reticulum 0.0033 0.0022 0.006 0.0038 0.0039 0.0015 0.0048 0.0011 0.0029 0.0051 0.0016 0.0018 0.0026 0.0018 0.0019 0.0011 0.0026 0.0013
Endosome 0.0093 0.0005 0.0021 0.001 0.0006 0.0012 0.0153 0.0004 0.007 0.0105 0.0083 0.0046 0.0042 0.0006 0.0019 0.0085 0.0125 0.0016
Golgi 0.0031 0.0003 0.0004 0.001 0.0003 0.0002 0.0048 0.0002 0.001 0.0014 0.0033 0.0014 0.0015 0.0002 0.0011 0.0146 0.0036 0.0007
Lipid Particles 0.0098 0.0002 0.0006 0.0004 0.0002 0.0001 0.0159 0.0001 0.001 0.0007 0.0029 0.0006 0.0027 0.0002 0.0022 0.0078 0.0052 0.0022
Mitochondria 0.0011 0.0002 0.0002 0.0011 0.0008 0.0001 0.0061 0.0003 0.0025 0.0025 0.0025 0.001 0.0005 0.0002 0.0007 0.0008 0.035 0.0017
None 0.4059 0.454 0.44 0.307 0.2407 0.3804 0.3773 0.483 0.4304 0.595 0.4297 0.3981 0.4349 0.4865 0.4265 0.5149 0.2508 0.4463
Nuclear Periphery 0.0108 0.0002 0.001 0.0008 0.0003 0.0002 0.0121 0.0004 0.0017 0.0026 0.0019 0.0011 0.0017 0.0003 0.0006 0.0003 0.0013 0.0003
Nucleolus 0.0004 0.0002 0.0001 0.0013 0.0001 0 0.0049 0.0007 0.0007 0.0041 0.001 0.0002 0.0008 0.0002 0.0003 0 0.0003 0.0004
Nucleus 0.0052 0.0007 0.0008 0.0026 0.0004 0.0005 0.0091 0.003 0.0027 0.0143 0.0018 0.0016 0.0012 0.001 0.0015 0.0004 0.0027 0.0009
Peroxisomes 0.0099 0.0001 0.0001 0.0004 0 0 0.0078 0.0002 0.001 0.001 0.0013 0.0009 0.0017 0.0001 0.0077 0.0071 0.002 0.0013
Punctate Nuclear 0.0031 0.0002 0.0004 0.0019 0.0001 0.0002 0.006 0.0063 0.0026 0.0059 0.0029 0.002 0.0033 0.0009 0.0013 0.0004 0.0028 0.0007
Vacuole 0.0012 0.001 0.0007 0.0014 0.0014 0.0004 0.0058 0.0008 0.0015 0.0019 0.0017 0.0006 0.001 0.0008 0.001 0.0004 0.0081 0.0006
Vacuole Periphery 0.0004 0.0001 0.0001 0.0002 0.0005 0 0.0014 0.0001 0.0005 0.0006 0.0005 0.0002 0.0002 0.0001 0.0001 0.0001 0.002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.4564 30.484 28.0063 37.931 24.7383 25.1557 37.7941 42.5967 28.8281 32.2449
Translational Efficiency 1.6125 1.1694 1.341 0.8818 1.2069 1.6936 1.0472 1.2373 1.9569 1.4928

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1174 491 2219 1495 1845 1571 619 128 3019 2062 2838 1623

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 743.78 846.40 989.80 1038.55 770.73 852.30 1023.26 1377.48 760.25 850.90 997.10 1065.28
Standard Deviation 103.15 177.82 175.52 176.72 143.47 162.15 176.84 210.02 129.96 166.03 176.35 201.47
Intensity Change Log 2 0.186464 0.412261 0.481623 0.145136 0.408875 0.837734 0.165580 0.410539 0.673787

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.004464 0.007373 0.001993 0.002710 0.003312 0.004661 0.000765 0.000168 0.003760 0.005307 0.001725 0.002510
Bud Neck 0.019428 0.025082 0.010276 0.005891 0.030422 0.030801 0.003516 0.012259 0.026147 0.029439 0.008801 0.006393
Bud Site 0.081082 0.096697 0.035625 0.034076 0.053814 0.070298 0.016344 0.008852 0.064418 0.076584 0.031420 0.032087
Cell Periphery 0.011116 0.007506 0.001728 0.001674 0.009394 0.005897 0.000770 0.000317 0.010063 0.006280 0.001519 0.001567
Cytoplasm 0.623034* 0.524591* 0.572717* 0.539734* 0.634843* 0.601566* 0.516205* 0.688798* 0.630251* 0.583237* 0.560391* 0.551490*
Cytoplasmic Foci 0.102027 0.161704 0.026448 0.051862 0.091584 0.078620 0.072968 0.002176 0.095645 0.098404 0.036594 0.047944
Eisosomes 0.004252 0.002371 0.000467 0.000296 0.005156 0.004114 0.000202 0.000066 0.004804 0.003699 0.000409 0.000277
Endoplasmic Reticulum 0.005857 0.002716 0.003567 0.006973 0.001889 0.001941 0.002617 0.000116 0.003432 0.002125 0.003360 0.006432
Endosome 0.003719 0.014419 0.001249 0.002835 0.004668 0.007961 0.006348 0.000087 0.004299 0.009498 0.002361 0.002618
Golgi 0.003996 0.005343 0.000158 0.000238 0.004163 0.004566 0.000179 0.000045 0.004098 0.004751 0.000162 0.000223
Lipid Particles 0.003926 0.005055 0.001585 0.002901 0.005141 0.003487 0.002548 0.000110 0.004668 0.003860 0.001795 0.002681
Mitochondria 0.001633 0.003128 0.000433 0.000259 0.003079 0.003601 0.000389 0.000510 0.002517 0.003488 0.000423 0.000278
Mitotic Spindle 0.000146 0.002445 0.003655 0.002183 0.000514 0.006332 0.001906 0.000059 0.000371 0.005406 0.003274 0.002016
None 0.005081 0.002077 0.002714 0.001612 0.004932 0.001903 0.004187 0.007577 0.004990 0.001945 0.003036 0.002082
Nuclear Periphery 0.000121 0.000156 0.000512 0.000493 0.000107 0.000134 0.000463 0.000075 0.000113 0.000139 0.000502 0.000460
Nuclear Periphery Foci 0.001049 0.001909 0.002134 0.006173 0.000840 0.001014 0.005320 0.000020 0.000921 0.001227 0.002829 0.005688
Nucleolus 0.000257 0.002226 0.000153 0.000132 0.000360 0.000610 0.000390 0.000099 0.000320 0.000995 0.000205 0.000130
Nucleus 0.078285 0.047550 0.241719* 0.169741* 0.091517 0.074820 0.166833* 0.245644* 0.086371 0.068326 0.225386* 0.175727*
Peroxisomes 0.002383 0.009925 0.000668 0.000396 0.002857 0.005551 0.000449 0.000078 0.002672 0.006592 0.000620 0.000371
Vacuole 0.047601 0.076724 0.092116 0.169715 0.050433 0.091241 0.197408 0.032903 0.049331 0.087784 0.115082 0.158925
Vacuole Periphery 0.000544 0.001003 0.000082 0.000106 0.000978 0.000884 0.000195 0.000040 0.000809 0.000912 0.000107 0.000101

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.68 6.03 4.84 4.64 -1.96 -2.82 7.33 10.76 11.97 4.00 -3.49 6.98 4.81 7.00 -2.53
Bud Neck -1.93 7.15 11.94 7.39 5.65 -0.11 17.54 4.06 4.09 -1.93 -1.86 15.13 17.93 16.19 3.47
Bud Site -1.82 10.52 11.02 9.48 0.34 -3.94 12.93 14.29 15.17 3.42 -3.09 13.07 12.97 13.55 -0.15
Cell Periphery 2.23 8.58 8.68 4.64 0.72 4.02 11.44 11.97 12.00 2.90 4.93 13.63 13.55 10.19 0.16
Cytoplasm 7.81 11.19 14.73 2.62 4.77 4.30 13.33 -1.91 -3.43 -8.58 7.21 17.69 16.51 9.21 1.86
Cytoplasmic Foci -7.01 21.54 14.20 13.97 -10.33 3.22 5.97 31.31 25.47 19.47 -0.79 24.85 18.68 15.49 -4.68
Eisosomes 3.54 12.08 12.70 4.61 2.51 1.91 12.72 13.03 9.87 4.21 2.70 16.39 16.98 10.41 2.41
Endoplasmic Reticulum 2.82 1.72 -1.10 -6.30 -5.61 -0.14 -3.81 9.26 6.86 10.46 2.81 -1.05 -5.22 -8.96 -5.46
Endosome -6.02 8.54 3.29 6.65 -8.40 -4.04 -1.81 10.68 11.07 12.62 -7.04 6.50 5.67 9.98 -1.15
Golgi -1.46 8.61 8.48 6.90 -3.50 -0.62 20.97 21.68 7.98 11.14 -1.19 18.87 18.66 9.75 -2.72
Lipid Particles -1.66 6.84 2.51 3.13 -7.49 2.24 3.60 7.54 9.36 13.06 1.60 6.23 4.08 2.91 -5.40
Mitochondria -1.70 3.05 3.58 3.65 2.69 -0.52 4.02 3.87 4.60 -0.95 -1.36 4.80 5.17 5.90 2.10
Mitotic Spindle -1.95 -4.73 -3.14 -0.23 0.76 -4.02 -1.87 2.51 4.38 2.23 -4.44 -4.77 -2.80 2.04 0.94
None 2.26 2.30 3.25 0.99 4.93 5.23 1.54 -1.07 -2.70 -1.76 5.13 3.90 5.39 0.36 2.83
Nuclear Periphery -1.32 -15.13 -11.08 -9.62 0.71 -1.31 -12.48 1.38 1.99 12.49 -1.57 -21.63 -12.53 -11.11 1.52
Nuclear Periphery Foci -3.62 -8.70 -15.19 -10.49 -11.02 -2.18 -11.52 16.95 13.90 13.53 -3.66 -14.90 -15.41 -14.06 -8.02
Nucleolus -2.07 2.71 3.99 2.22 1.32 -1.53 0.42 7.32 3.09 6.21 -2.62 4.06 7.29 3.39 3.25
Nucleus 5.20 -28.03 -14.25 -17.12 11.28 4.00 -7.71 -7.40 -8.17 -3.65 5.29 -28.86 -15.60 -18.39 8.45
Peroxisomes -5.26 8.39 13.10 6.68 1.18 -2.98 9.18 10.75 6.36 8.32 -5.18 10.23 13.56 8.45 1.50
Vacuole -6.09 -18.98 -29.02 -19.39 -14.77 -11.77 -20.54 1.60 7.95 18.72 -13.52 -26.88 -28.65 -20.12 -9.27
Vacuole Periphery -1.89 2.82 2.73 4.96 -1.06 0.29 2.75 3.22 4.68 4.70 -0.42 3.72 3.78 5.68 0.77
Standard name
Human Ortholog
Description Serine-rich protein of unknown function; predicted to be palmitoylated; overproduction suppresses growth inhibition caused by exposure to immunosuppressant leflunomide; MLF3 has a paralog, VHS2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (66%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-10

Mlf3

Mlf3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mlf3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available