Standard name
Human Ortholog
Description Pleiotropic negative transcriptional regulator; involved in Ras-CAMP and lysine biosynthetic pathways and nitrogen regulation; involved in retrograde (RTG) mitochondria-to-nucleus signaling
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0 0.07 0 0 0 0 0 0
Cytoplasm 0.88 0.95 – 0.97 0.97 0.97 0.96 0.93 0.83 0.88 0.71 0.83 0.97 1.0 0.99 0.94 0.96 0.94 0.59 0.7 0.71 0.82 0.85 0.83
Endoplasmic Reticulum 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0
Endosome 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.06 0 0 0 0
Golgi 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0 – 0 0 0 0 0.05 0.21 0.13 0.36 0.24 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – 0 0 0 0 0 0.05 0 0 0 0 0 0 0.05 0 0 0.24 0.18 0.13 0.06 0.08 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 2 1 3 5 1 5 5 1 1 0 0 0 0 3 0 1 0 0 0 0
Bud 0 0 – 3 1 0 1 1 7 8 3 2 0 0 0 1 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 – 2 2 1 7 6 2 4 3 6 1 5 3 32 15 26 0 0 0 0 0 0
Cytoplasm 104 152 – 198 248 261 370 403 221 280 187 214 58 131 168 288 406 359 77 128 13 26 87 87
Endoplasmic Reticulum 5 4 – 4 1 4 4 10 0 4 5 2 1 0 1 20 23 12 1 0 0 0 1 1
Endosome 1 0 – 1 3 0 2 3 3 2 1 2 2 0 0 2 0 2 11 11 0 1 0 0
Golgi 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0
Mitochondria 8 0 – 0 2 6 12 23 57 41 95 63 0 0 0 3 4 6 3 4 0 0 0 0
Nucleus 0 0 – 0 0 0 0 0 2 0 1 6 0 0 0 1 5 4 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 – 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 1 0 2 1 1 1 3 0 0 0 1 0 0 1 0 0 0 0 0 0
Vac/Vac Membrane 4 6 – 1 3 3 3 4 12 7 1 3 0 0 4 14 12 13 31 32 2 1 8 11
Unique Cell Count 118 160 204 255 268 387 434 266 320 263 259 60 131 170 308 421 380 131 183 19 32 103 105
Labelled Cell Count 122 163 209 263 276 404 456 306 352 306 301 63 137 177 361 466 426 131 183 19 32 103 105


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.9 6.8 – 6.7 7.2 6.3 6.1 6.5 5.7 5.7 5.3 5.6 6.7 7.0 7.4 8.8 8.6 7.9 – 6.6 7.0
Std Deviation (1e-4) 0.7 0.8 – 1.3 1.6 1.4 1.0 1.4 1.3 1.1 1.1 1.1 1.3 1.3 2.0 1.3 1.3 1.3 – 1.5 1.5
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.7063 3.1388 2.6733 2.3448 2.7786 2.6966 1.4441 2.1797 2.0902 1.5043 1.7071 1.7081 -0.5327 0.318 0.0233 -0.1946 -0.3062 0.0621
Actin 0.025 0.0044 0.0086 0.0075 0.0125 0.0076 0.0325 0.0006 0.0055 0.0315 0.0693 0.0015 0.0081 0.0004 0.0106 0.0058 0.0374 0.0046
Bud 0.0079 0.0071 0.0123 0.0052 0.0135 0.003 0.0007 0.0007 0.0004 0.0024 0.0007 0.0003 0.0034 0.0035 0.0014 0.0013 0.0008 0.0002
Bud Neck 0.0091 0.0011 0.0014 0.004 0.0049 0.0015 0.0007 0.0001 0.0009 0.0003 0.0006 0.0004 0.0017 0.0001 0.0014 0.0004 0.0021 0.0009
Bud Periphery 0.0038 0.0023 0.0052 0.0056 0.0153 0.0025 0.0008 0.0002 0.0003 0.0061 0.0004 0.0002 0.0016 0.0011 0.0021 0.0027 0.0011 0.0003
Bud Site 0.0144 0.0171 0.026 0.0073 0.0129 0.0034 0.0046 0.0023 0.0028 0.003 0.0012 0.0006 0.0016 0.0043 0.0046 0.003 0.004 0.0004
Cell Periphery 0.0011 0.0008 0.0006 0.0002 0.0006 0.0002 0.0007 0.0002 0.0001 0.0003 0.0001 0.0001 0.0002 0.0003 0.0004 0.0002 0.0003 0.0001
Cytoplasm 0.5537 0.7592 0.6577 0.6919 0.4595 0.7853 0.5869 0.8097 0.7413 0.7345 0.6206 0.8138 0.5341 0.6921 0.72 0.5206 0.5601 0.7572
Cytoplasmic Foci 0.0511 0.0276 0.0348 0.0507 0.0773 0.0256 0.055 0.0108 0.0214 0.0206 0.0465 0.0153 0.0247 0.0203 0.0201 0.0173 0.0574 0.033
Eisosomes 0.0009 0 0.0001 0 0.0002 0 0.0003 0 0 0.0002 0.0001 0 0.0008 0 0.0001 0.0003 0.0004 0
Endoplasmic Reticulum 0.0102 0.0031 0.0056 0.0021 0.0068 0.0027 0.0219 0.0037 0.0046 0.0048 0.0032 0.0048 0.0059 0.0059 0.0063 0.0047 0.0041 0.0151
Endosome 0.0565 0.025 0.045 0.0568 0.1452 0.0286 0.0569 0.0237 0.0415 0.0505 0.0645 0.0287 0.0332 0.0264 0.0412 0.0472 0.0614 0.078
Golgi 0.0074 0.0027 0.0049 0.0078 0.0175 0.0024 0.0196 0.0017 0.0094 0.0076 0.0401 0.0108 0.0043 0.0008 0.0018 0.0102 0.0504 0.0107
Lipid Particles 0.01 0.0004 0.0029 0.0011 0.0151 0.0015 0.0117 0.0002 0.0037 0.0173 0.0218 0.0012 0.0168 0.0005 0.001 0.0051 0.0455 0.0062
Mitochondria 0.0143 0.0017 0.0041 0.0014 0.0101 0.0049 0.0185 0.0005 0.0039 0.0132 0.0057 0.0193 0.0057 0.0005 0.0014 0.0123 0.0424 0.0062
None 0.192 0.1245 0.1505 0.1243 0.1323 0.1116 0.1428 0.1342 0.1465 0.076 0.1054 0.0927 0.2959 0.2344 0.1641 0.3476 0.0127 0.0682
Nuclear Periphery 0.006 0.0021 0.0042 0.0012 0.0196 0.0022 0.0062 0.0007 0.0021 0.0017 0.0007 0.0006 0.0089 0.0015 0.0019 0.003 0.0034 0.0022
Nucleolus 0.0021 0.0004 0.0013 0.0011 0.002 0.0006 0.0005 0 0.0002 0.0001 0.0001 0.0001 0.0021 0.0001 0.0003 0.0004 0.0014 0.0001
Nucleus 0.0047 0.0032 0.0043 0.003 0.0104 0.0028 0.0026 0.0007 0.0015 0.0011 0.0007 0.0007 0.008 0.0008 0.002 0.0022 0.0041 0.0009
Peroxisomes 0.0058 0.0005 0.0083 0.0023 0.007 0.0017 0.007 0.0001 0.004 0.0114 0.0107 0.0012 0.0127 0.0001 0.0031 0.0022 0.0825 0.0064
Punctate Nuclear 0.0035 0.002 0.0055 0.0026 0.0077 0.0036 0.0083 0.0002 0.0015 0.0006 0.0006 0.0001 0.0145 0.0004 0.0009 0.0016 0.0167 0.0007
Vacuole 0.0173 0.0124 0.0142 0.0207 0.024 0.0068 0.0194 0.0088 0.0071 0.0136 0.0054 0.0057 0.0142 0.0055 0.0134 0.0095 0.0089 0.0066
Vacuole Periphery 0.0032 0.0023 0.0024 0.003 0.0056 0.0015 0.0022 0.0009 0.0012 0.003 0.0013 0.0018 0.0016 0.001 0.0019 0.0024 0.0029 0.002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.8319 13.4889 16.2652 16.3524 15.8957 9.4256 14.0648 17.2597 14.5151 17.7157
Translational Efficiency 0.824 0.8329 0.7363 0.6975 0.7369 1.3478 0.8548 0.7411 0.9362 0.7906

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1544 802 1499 1366 227 1695 1488 2206 1771 2497 2987 3572

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 703.48 802.81 1033.56 910.70 717.04 722.06 963.70 974.34 705.22 748.00 998.76 950.00
Standard Deviation 80.05 122.45 115.12 121.07 106.70 85.93 111.45 120.37 84.06 106.06 118.57 124.54
Intensity Change Log 2 — 0.190549 0.555041 0.372466 — 0.010065 0.426531 0.442372 — 0.102267 0.491603 0.408176

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000257 0.000461 0.000192 0.000782 0.000151 0.000258 0.000168 0.001162 0.000244 0.000323 0.000180 0.001017
Bud Neck 0.014380 0.018233 0.001544 0.005774 0.005170 0.015705 0.000862 0.002585 0.013199 0.016517 0.001204 0.003804
Bud Site 0.009998 0.031220 0.009093 0.027043 0.005031 0.022577 0.005061 0.030174 0.009362 0.025353 0.007084 0.028976
Cell Periphery 0.000132 0.000126 0.000066 0.000067 0.000122 0.000070 0.000056 0.000046 0.000131 0.000088 0.000061 0.000054
Cytoplasm 0.423445* 0.339558* 0.792568* 0.658784* 0.466770* 0.287330* 0.785828* 0.817073* 0.428998* 0.304105* 0.789211* 0.756541*
Cytoplasmic Foci 0.245492* 0.299927* 0.033905 0.060497 0.253222* 0.277872* 0.041606 0.025819 0.246483* 0.284955* 0.037742 0.039081
Eisosomes 0.000116 0.000107 0.000012 0.000017 0.000086 0.000063 0.000015 0.000008 0.000113 0.000077 0.000014 0.000012
Endoplasmic Reticulum 0.002682 0.001137 0.000768 0.000396 0.003109 0.000944 0.000702 0.000102 0.002737 0.001006 0.000735 0.000215
Endosome 0.063645 0.086243 0.004626 0.011977 0.044343 0.135481 0.003893 0.002162 0.061171 0.119666 0.004261 0.005916
Golgi 0.006415 0.011653 0.000301 0.005100 0.001878 0.013732 0.000282 0.001807 0.005834 0.013064 0.000291 0.003066
Lipid Particles 0.008888 0.004229 0.000845 0.000804 0.009099 0.005514 0.000781 0.000212 0.008915 0.005101 0.000813 0.000439
Mitochondria 0.003141 0.006305 0.000102 0.001007 0.000701 0.008217 0.000066 0.001209 0.002828 0.007603 0.000084 0.001131
Mitotic Spindle 0.002568 0.003926 0.004153 0.020960 0.000720 0.005985 0.008146 0.023664 0.002331 0.005324 0.006142 0.022630
None 0.002121 0.000341 0.001931 0.001703 0.003674 0.000237 0.002053 0.001473 0.002320 0.000270 0.001992 0.001561
Nuclear Periphery 0.000252 0.000135 0.000295 0.000297 0.000911 0.000141 0.000278 0.000074 0.000337 0.000139 0.000287 0.000159
Nuclear Periphery Foci 0.000639 0.000434 0.004098 0.002352 0.003475 0.000264 0.003430 0.000429 0.001003 0.000319 0.003765 0.001165
Nucleolus 0.000915 0.001061 0.000129 0.000316 0.001280 0.000637 0.000104 0.000106 0.000962 0.000773 0.000117 0.000186
Nucleus 0.024386 0.011343 0.060223 0.018193 0.042096 0.007960 0.044130 0.018527 0.026656 0.009047 0.052206 0.018399
Peroxisomes 0.002635 0.007338 0.000329 0.001734 0.002090 0.006090 0.000413 0.001165 0.002565 0.006491 0.000371 0.001383
Vacuole 0.181503 0.168448 0.084555 0.177930 0.151067 0.198302 0.101978 0.070657 0.177602 0.188713 0.093234 0.111680
Vacuole Periphery 0.006389 0.007777 0.000265 0.004268 0.005006 0.012624 0.000145 0.001546 0.006212 0.011067 0.000205 0.002587

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.52 0.30 -0.99 0.32 -1.83 -1.66 -0.54 -1.71 -1.28 -1.63 -0.68 0.42 -1.44 -1.22 -2.02
Bud Neck -2.24 12.11 9.10 9.84 -3.02 -9.57 5.54 4.29 16.48 -4.36 -2.88 13.04 11.39 18.59 -4.22
Bud Site -10.00 1.19 -3.43 6.04 -4.21 -10.63 0.37 -7.16 2.66 -7.32 -10.76 2.77 -5.12 5.26 -7.85
Cell Periphery 0.52 6.10 8.06 6.86 3.90 4.49 6.11 8.02 5.36 5.31 4.39 7.70 10.24 8.34 6.89
Cytoplasm 7.30 -36.46 -20.31 -23.55 12.46 9.66 -13.63 -16.46 -60.03* -5.66 15.49 -40.47 -37.69 -59.82 3.37
Cytoplasmic Foci -6.11 44.05 39.35 32.68 -6.34 -2.07 18.45 19.72 50.44 7.42 -6.48 47.74 48.19 59.46 1.46
Eisosomes 0.84 12.95 12.80 9.44 -1.15 3.25 9.92 11.26 18.63 10.74 4.61 13.97 14.67 17.75 7.18
Endoplasmic Reticulum 4.13 5.19 6.92 5.90 8.48 2.44 2.67 3.42 9.81 11.61 5.24 5.96 8.00 11.16 14.77
Endosome -4.63 24.70 22.84 19.10 -2.27 -16.07 9.74 10.26 35.11 8.07 -15.97 26.60 26.74 39.30 1.74
Golgi -4.60 12.21 5.43 8.10 -3.43 -13.87 5.34 2.30 14.83 -1.65 -9.32 12.64 8.31 16.65 -3.58
Lipid Particles 5.04 14.66 14.88 4.59 1.88 2.40 5.63 6.00 14.26 9.95 6.25 15.81 16.68 13.22 6.81
Mitochondria -2.26 5.63 4.73 4.58 -2.45 -7.39 3.30 0.57 7.57 -3.12 -5.14 5.81 4.74 8.86 -3.91
Mitotic Spindle -0.73 -0.95 -3.91 -3.27 -3.63 -3.85 -4.03 -6.53 -3.14 -2.07 -2.08 -2.55 -6.00 -4.31 -4.05
None 4.15 0.92 1.85 -4.06 2.10 1.60 0.90 1.09 -11.18 2.56 4.51 1.26 2.17 -10.01 3.43
Nuclear Periphery 1.98 -3.00 -0.84 -3.69 2.45 2.36 1.49 2.33 -0.54 13.81 3.08 -1.32 1.96 -3.38 10.31
Nuclear Periphery Foci 1.29 -12.93 -4.61 -5.69 7.83 2.66 -0.03 2.48 -4.62 13.55 3.58 -11.77 0.11 -7.04 15.39
Nucleolus -0.31 6.82 5.47 1.43 -2.34 1.56 3.03 2.98 3.10 -2.61 0.71 7.54 7.02 2.76 -2.70
Nucleus 6.42 -17.96 -1.49 -8.00 17.58 6.62 -2.23 2.69 -21.05 13.60 12.56 -18.77 -0.80 -20.86 22.12
Peroxisomes -8.28 9.06 5.24 11.69 -3.03 -6.91 4.05 3.27 13.07 -2.34 -10.05 9.70 7.28 17.13 -3.92
Vacuole 1.31 9.29 -8.72 -8.71 -15.79 -4.09 -0.69 0.92 9.50 2.83 -2.28 6.90 -0.92 1.45 -8.26
Vacuole Periphery -1.76 13.77 6.87 7.59 -3.55 -3.18 2.10 1.85 16.28 -1.79 -6.92 12.35 9.14 17.69 -3.69
Standard name
Human Ortholog
Description Pleiotropic negative transcriptional regulator; involved in Ras-CAMP and lysine biosynthetic pathways and nitrogen regulation; involved in retrograde (RTG) mitochondria-to-nucleus signaling
Localization
Cell Percentages cytoplasm (91%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Mks1

Mks1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mks1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available