Standard name | |
---|---|
Human Ortholog | |
Description | Protein localized in the bud neck at G2/M phase; physically interacts with septins; possibly involved in a mitotic signaling network |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 26 | 11 | 20 | 11 | 28 | 3 | 10 | 4 | 7 | 13 | 2 | 6 | 61 | 72 | 72 | 54 | 51 | 48 | 11 | 21 | 17 | 13 | 23 | 31 |
Bud | 11 | 7 | 12 | 13 | 19 | 21 | 42 | 46 | 31 | 30 | 22 | 17 | 20 | 30 | 42 | 25 | 27 | 19 | 24 | 22 | 21 | 42 | 48 | 75 |
Bud Neck | 0 | 0 | 0 | 2 | 1 | 0 | 5 | 2 | 1 | 2 | 0 | 1 | 2 | 11 | 10 | 4 | 24 | 2 | 38 | 36 | 38 | 93 | 123 | 131 |
Bud Site | 27 | 5 | 20 | 20 | 42 | 33 | 47 | 44 | 24 | 19 | 22 | 29 | 39 | 35 | 39 | 44 | 49 | 31 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 3 | 10 | 3 | 1 | 0 | 0 | 0 | 0 | 2 |
Cytoplasm | 6 | 4 | 7 | 8 | 13 | 30 | 28 | 34 | 23 | 25 | 15 | 20 | 28 | 64 | 90 | 50 | 64 | 40 | 4 | 1 | 1 | 4 | 8 | 17 |
Endoplasmic Reticulum | 1 | 1 | 0 | 1 | 3 | 1 | 1 | 0 | 0 | 1 | 1 | 0 | 4 | 7 | 4 | 4 | 8 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 2 | 6 | 5 | 1 | 0 | 2 | 7 | 2 | 0 | 0 | 0 | 1 | 3 | 0 | 7 | 9 | 1 | 1 | 1 | 1 | 3 | 5 | 5 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 6 | 3 | 0 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | 30 | 1 | 7 | 9 | 32 | 24 | 66 | 90 | 73 | 109 | 116 | 92 | 2 | 0 | 0 | 31 | 16 | 10 | 0 | 1 | 2 | 1 | 2 | 6 |
Nucleus | 1 | 0 | 0 | 4 | 4 | 3 | 14 | 17 | 5 | 1 | 8 | 1 | 3 | 1 | 5 | 8 | 11 | 2 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 |
Nucleolus | 1 | 1 | 1 | 1 | 0 | 3 | 3 | 8 | 3 | 3 | 3 | 5 | 0 | 1 | 0 | 2 | 5 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
Peroxisomes | 14 | 9 | 27 | 21 | 33 | 39 | 38 | 40 | 23 | 17 | 16 | 16 | 9 | 24 | 16 | 32 | 12 | 14 | 1 | 0 | 0 | 1 | 0 | 0 |
SpindlePole | 1 | 2 | 10 | 11 | 15 | 19 | 31 | 43 | 23 | 5 | 13 | 12 | 12 | 71 | 55 | 48 | 32 | 19 | 0 | 0 | 1 | 1 | 1 | 0 |
Vac/Vac Membrane | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 1 | 2 | 0 | 0 | 0 | 1 | 5 | 3 | 7 | 5 | 0 | 0 | 0 | 2 | 3 | 4 |
Unique Cell Count | 58 | 29 | 58 | 57 | 106 | 117 | 168 | 213 | 140 | 153 | 151 | 126 | 123 | 231 | 251 | 196 | 210 | 136 | 85 | 87 | 86 | 172 | 222 | 282 |
Labelled Cell Count | 118 | 43 | 111 | 106 | 191 | 177 | 288 | 338 | 216 | 229 | 219 | 200 | 181 | 322 | 339 | 318 | 331 | 200 | 85 | 87 | 86 | 172 | 222 | 282 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.2 | 7.0 | 5.7 | 5.2 | 5.4 | 4.6 | 4.1 | 3.8 | 3.7 | 3.5 | 3.0 | 3.1 | 6.9 | 6.5 | 6.0 | 7.5 | 7.4 | 7.2 | 7.3 | 7.8 | 8.0 |
Std Deviation (1e-4) | 0.8 | 1.2 | 2.1 | 1.9 | 2.8 | 2.7 | 2.2 | 1.5 | 1.6 | 1.4 | 1.0 | 1.1 | 1.1 | 1.1 | 1.2 | 1.9 | 2.5 | 1.7 | 1.4 | 1.2 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.14 | -0.09 | -0.33 | -0.5 | -0.6 | -0.62 | -0.7 | -0.93 | -0.88 | 0.26 | 0.17 | 0.06 | 0.39 | 0.36 | 0.32 | 0.35 | 0.45 | 0.47 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -1.8 | -1.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.9 | -0.5 | -0.9 | -1.0 | -1.6 | 0.1 |
Bud | 0.3 | -0.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.7 | -1.5 | -0.7 | -1.5 | -1.5 | -1.2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.7 | 0 |
Bud Site | 0.1 | 0.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.4 | -3.4 | -3.3 | -1.9 | -1.7 | -1.7 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.3 | 0 | 2.1 | 0.8 | 0.7 | 0.8 | 0.8 | -0.5 | 0.7 | 1.7 | 2.5 | 3.5 | 2.2 | 2.8 | 2.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -0.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.6 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.7 | 0 | -1.1 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | -1.1 | -2.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -6.2 | -6.5 | -8.0 | -4.8 | -7.8 | -5.7 |
SpindlePole | 0.3 | -0.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.4 | 2.0 | 0.8 | 1.2 | -0.4 | -0.6 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.3491 | 2.5237 | 1.1358 | 0.4307 | 0.4923 | 1.6963 | 1.9487 | 2.5466 | 1.6552 | 1.2582 | 0.409 | 2.1554 | 1.1482 | 1.5674 | 0.4105 | 0.321 | 0.0612 | 1.3262 |
Actin | 0.0476 | 0.0829 | 0.0398 | 0.0269 | 0.0276 | 0.0653 | 0.1974 | 0.0662 | 0.079 | 0.0316 | 0.0326 | 0.0755 | 0.0816 | 0.0701 | 0.0301 | 0.031 | 0.0041 | 0.072 |
Bud | 0.0045 | 0.0145 | 0.006 | 0.0029 | 0.0015 | 0.0022 | 0.0056 | 0.0048 | 0.0057 | 0.0035 | 0.0026 | 0.0014 | 0.0072 | 0.0155 | 0.0054 | 0.0024 | 0.0034 | 0.0023 |
Bud Neck | 0.2635 | 0.0211 | 0.4277 | 0.6457 | 0.6513 | 0.8684 | 0.2029 | 0.0271 | 0.3676 | 0.6046 | 0.4847 | 0.8333 | 0.2692 | 0.0463 | 0.4702 | 0.7811 | 0.7765 | 0.8755 |
Bud Periphery | 0.0067 | 0.0309 | 0.0128 | 0.0048 | 0.0037 | 0.0045 | 0.0104 | 0.0056 | 0.0062 | 0.0057 | 0.0049 | 0.0037 | 0.01 | 0.0129 | 0.0082 | 0.0054 | 0.0048 | 0.004 |
Bud Site | 0.4744 | 0.7855 | 0.2946 | 0.0904 | 0.0391 | 0.0165 | 0.322 | 0.7259 | 0.3435 | 0.1135 | 0.0665 | 0.0083 | 0.4995 | 0.8177 | 0.3672 | 0.0821 | 0.1056 | 0.0185 |
Cell Periphery | 0.0038 | 0.0094 | 0.0037 | 0.0017 | 0.004 | 0.0018 | 0.0044 | 0.0054 | 0.0031 | 0.0048 | 0.0099 | 0.0015 | 0.0046 | 0.0042 | 0.0022 | 0.0016 | 0.0017 | 0.0011 |
Cytoplasm | 0.0439 | 0.0196 | 0.05 | 0.0177 | 0.0041 | 0.0056 | 0.0802 | 0.1146 | 0.0906 | 0.0684 | 0.0323 | 0.0248 | 0.046 | 0.027 | 0.0362 | 0.0062 | 0.0954 | 0.0103 |
Cytoplasmic Foci | 0.0399 | 0.0045 | 0.0298 | 0.0584 | 0.1059 | 0.0157 | 0.0157 | 0.0032 | 0.0219 | 0.0614 | 0.1972 | 0.0125 | 0.0338 | 0.0018 | 0.024 | 0.0362 | 0.0029 | 0.0086 |
Eisosomes | 0.0014 | 0.0002 | 0.0022 | 0.0004 | 0.0005 | 0.0003 | 0.0013 | 0.0002 | 0.0004 | 0.0002 | 0.0007 | 0.0002 | 0.0005 | 0.0002 | 0.0003 | 0.0002 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0025 | 0.0014 | 0.0009 | 0.0004 | 0.0001 | 0.0001 | 0.0052 | 0.001 | 0.0012 | 0.0017 | 0.0013 | 0.0007 | 0.0024 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0002 |
Endosome | 0.0079 | 0.0004 | 0.0011 | 0.0011 | 0.0024 | 0.002 | 0.0185 | 0.0009 | 0.0114 | 0.0437 | 0.0165 | 0.0091 | 0.0039 | 0.0002 | 0.0017 | 0.0002 | 0.0004 | 0.0018 |
Golgi | 0.0026 | 0.0007 | 0.0014 | 0.0019 | 0.0048 | 0.0023 | 0.0148 | 0.0011 | 0.0102 | 0.0181 | 0.0268 | 0.0063 | 0.0021 | 0.0008 | 0.001 | 0.0007 | 0.0005 | 0.0016 |
Lipid Particles | 0.0034 | 0.0001 | 0.0007 | 0.0016 | 0.0036 | 0.0005 | 0.0217 | 0.0002 | 0.0062 | 0.002 | 0.003 | 0.0072 | 0.0016 | 0.0002 | 0.0004 | 0.0002 | 0.0002 | 0.0002 |
Mitochondria | 0.0023 | 0.0014 | 0.0009 | 0.0014 | 0.0024 | 0.0006 | 0.0127 | 0.0002 | 0.0125 | 0.0059 | 0.0016 | 0.0006 | 0.0009 | 0.0003 | 0.0003 | 0.0003 | 0.0001 | 0.0001 |
None | 0.0728 | 0.0205 | 0.1139 | 0.1193 | 0.07 | 0.0069 | 0.0101 | 0.0411 | 0.0263 | 0.0038 | 0.0845 | 0.0038 | 0.0237 | 0.0005 | 0.0353 | 0.0445 | 0.0015 | 0.0003 |
Nuclear Periphery | 0.002 | 0.0003 | 0.0003 | 0.0002 | 0 | 0 | 0.0203 | 0.0003 | 0.0014 | 0.0008 | 0.0003 | 0.001 | 0.0037 | 0.0001 | 0.0002 | 0 | 0 | 0.0001 |
Nucleolus | 0.0029 | 0.0003 | 0.0015 | 0.0013 | 0.0047 | 0.0003 | 0.008 | 0.0002 | 0.0007 | 0.0004 | 0.0006 | 0.0002 | 0.0005 | 0.0001 | 0.0008 | 0.0005 | 0.0005 | 0.0001 |
Nucleus | 0.0026 | 0.0008 | 0.0015 | 0.0014 | 0.0012 | 0.0002 | 0.0135 | 0.0006 | 0.0011 | 0.0009 | 0.001 | 0.0026 | 0.0017 | 0.0004 | 0.0013 | 0.0003 | 0.0005 | 0.0002 |
Peroxisomes | 0.0085 | 0.0044 | 0.0076 | 0.0174 | 0.063 | 0.0058 | 0.0205 | 0.0003 | 0.0079 | 0.0055 | 0.0128 | 0.0021 | 0.0029 | 0.0008 | 0.0021 | 0.0019 | 0.0007 | 0.0021 |
Punctate Nuclear | 0.0048 | 0.0002 | 0.0028 | 0.0046 | 0.009 | 0.0008 | 0.0088 | 0.0003 | 0.0021 | 0.0038 | 0.0177 | 0.0047 | 0.0027 | 0.0004 | 0.012 | 0.0047 | 0.0002 | 0.0002 |
Vacuole | 0.0018 | 0.0009 | 0.0007 | 0.0005 | 0.0011 | 0.0002 | 0.004 | 0.0006 | 0.001 | 0.0159 | 0.0025 | 0.0004 | 0.0014 | 0.0003 | 0.0007 | 0.0003 | 0.0005 | 0.0006 |
Vacuole Periphery | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.002 | 0.0001 | 0.0003 | 0.0036 | 0.0003 | 0.0001 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 7.6625 | 12.0879 | 23.9194 | 87.8736 | 41.4924 | 5.4059 | 7.3333 | 10.1739 | 58.5611 | 46.0577 |
Translational Efficiency | 0.3315 | 0.1731 | 1.0269 | 0.6724 | 0.7465 | 0.612 | 0.4634 | 0.6127 | 0.9742 | 0.9582 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
168 | 1318 | 1991 | 1243 | 173 | 2059 | 482 | 1623 | 341 | 3377 | 2473 | 2866 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 764.77 | 791.11 | 908.96 | 856.92 | 783.80 | 814.17 | 1000.90 | 835.87 | 774.42 | 805.17 | 926.88 | 845.00 |
Standard Deviation | 100.04 | 123.78 | 118.05 | 105.86 | 84.89 | 101.12 | 114.99 | 116.72 | 93.15 | 111.09 | 122.97 | 112.62 |
Intensity Change Log 2 | — | 0.048852 | 0.249191 | 0.164135 | — | 0.054844 | 0.352740 | 0.092793 | — | 0.051888 | 0.302530 | 0.128466 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.002814 | 0.012083 | 0.004956 | 0.006158 | 0.003904 | 0.005168 | 0.007792 | 0.004371 | 0.003367 | 0.007867 | 0.005509 | 0.005146 |
Bud Neck | 0.369683* | 0.175280 | 0.262455* | 0.260103* | 0.253336* | 0.310905* | 0.239840* | 0.227753* | 0.310657* | 0.257972* | 0.258047* | 0.241783* |
Bud Site | 0.435719* | 0.583484* | 0.541247* | 0.578881* | 0.441911* | 0.543018* | 0.557354* | 0.562971* | 0.438860* | 0.558811* | 0.544386* | 0.569872* |
Cell Periphery | 0.005129 | 0.004236 | 0.001554 | 0.000743 | 0.004467 | 0.004720 | 0.001264 | 0.000835 | 0.004793 | 0.004531 | 0.001497 | 0.000795 |
Cytoplasm | 0.052212 | 0.093779 | 0.096658 | 0.058535 | 0.106500* | 0.061446 | 0.058091 | 0.097196 | 0.079754 | 0.074065 | 0.089141 | 0.080429 |
Cytoplasmic Foci | 0.042975 | 0.041274 | 0.010938 | 0.014573 | 0.081263 | 0.018926 | 0.016172 | 0.018958 | 0.062400 | 0.027648 | 0.011958 | 0.017057 |
Eisosomes | 0.000239 | 0.000254 | 0.000126 | 0.000101 | 0.000299 | 0.000197 | 0.000193 | 0.000100 | 0.000270 | 0.000219 | 0.000140 | 0.000101 |
Endoplasmic Reticulum | 0.000107 | 0.000356 | 0.000443 | 0.000547 | 0.001013 | 0.000175 | 0.001134 | 0.000320 | 0.000567 | 0.000246 | 0.000578 | 0.000418 |
Endosome | 0.028966 | 0.018271 | 0.006023 | 0.008569 | 0.014573 | 0.010354 | 0.005881 | 0.009966 | 0.021664 | 0.013444 | 0.005995 | 0.009360 |
Golgi | 0.006998 | 0.006099 | 0.001303 | 0.001076 | 0.003070 | 0.003628 | 0.000593 | 0.001416 | 0.005005 | 0.004593 | 0.001165 | 0.001268 |
Lipid Particles | 0.001773 | 0.001092 | 0.000581 | 0.000616 | 0.002683 | 0.000438 | 0.001248 | 0.000301 | 0.002235 | 0.000693 | 0.000711 | 0.000437 |
Mitochondria | 0.015040 | 0.014767 | 0.001492 | 0.001657 | 0.008339 | 0.010958 | 0.000909 | 0.002118 | 0.011640 | 0.012445 | 0.001379 | 0.001918 |
Mitotic Spindle | 0.002297 | 0.003256 | 0.006618 | 0.007907 | 0.002126 | 0.002904 | 0.004698 | 0.009699 | 0.002210 | 0.003041 | 0.006244 | 0.008922 |
None | 0.001935 | 0.000364 | 0.000910 | 0.000287 | 0.000674 | 0.000591 | 0.000296 | 0.000182 | 0.001296 | 0.000502 | 0.000790 | 0.000227 |
Nuclear Periphery | 0.000023 | 0.000025 | 0.000077 | 0.000089 | 0.000080 | 0.000016 | 0.000137 | 0.000053 | 0.000052 | 0.000019 | 0.000089 | 0.000069 |
Nuclear Periphery Foci | 0.000109 | 0.000189 | 0.000283 | 0.000614 | 0.000808 | 0.000081 | 0.000889 | 0.000301 | 0.000464 | 0.000123 | 0.000401 | 0.000437 |
Nucleolus | 0.000232 | 0.001168 | 0.000234 | 0.000265 | 0.001872 | 0.000069 | 0.000247 | 0.000226 | 0.001064 | 0.000498 | 0.000237 | 0.000243 |
Nucleus | 0.006450 | 0.003767 | 0.023078 | 0.010468 | 0.019334 | 0.003238 | 0.026421 | 0.013362 | 0.012987 | 0.003445 | 0.023729 | 0.012107 |
Peroxisomes | 0.013288 | 0.020587 | 0.012651 | 0.022601 | 0.034429 | 0.012127 | 0.016372 | 0.016569 | 0.024013 | 0.015429 | 0.013376 | 0.019185 |
Vacuole | 0.012804 | 0.017656 | 0.028094 | 0.025805 | 0.018157 | 0.010059 | 0.060250 | 0.032703 | 0.015520 | 0.013024 | 0.034361 | 0.029711 |
Vacuole Periphery | 0.001207 | 0.002015 | 0.000277 | 0.000404 | 0.001161 | 0.000982 | 0.000219 | 0.000601 | 0.001184 | 0.001385 | 0.000266 | 0.000515 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -5.80 | -2.45 | -2.81 | 3.37 | -1.10 | -1.31 | -2.56 | -0.67 | 0.91 | 2.39 | -5.34 | -2.92 | -2.59 | 3.17 | 0.34 |
Bud Neck | 8.04 | 4.72 | 5.02 | -7.66 | 0.93 | -2.65 | 1.46 | 1.50 | 10.87 | -0.15 | 3.02 | 3.60 | 4.69 | 4.29 | 2.55 |
Bud Site | -5.94 | -3.83 | -4.82 | 2.35 | -2.37 | -3.93 | -2.36 | -3.77 | 0.32 | -1.89 | -6.70 | -4.74 | -5.98 | 1.61 | -2.54 |
Cell Periphery | 0.71 | 3.12 | 3.83 | 6.84 | 6.80 | -0.29 | 4.45 | 5.04 | 9.03 | 3.23 | 0.35 | 4.90 | 5.95 | 11.35 | 7.14 |
Cytoplasm | -3.79 | -4.79 | -1.60 | 3.72 | 5.45 | 3.11 | 2.41 | -0.06 | -8.06 | -4.87 | 0.71 | -1.70 | -1.16 | -4.09 | 1.06 |
Cytoplasmic Foci | 0.20 | 3.44 | 2.99 | 7.23 | -2.11 | 5.13 | 5.01 | 4.98 | -0.86 | -0.35 | 4.53 | 6.37 | 5.71 | 4.74 | -3.56 |
Eisosomes | -0.25 | 3.04 | 3.64 | 3.56 | 2.66 | 2.76 | 2.37 | 5.43 | 5.21 | 5.93 | 1.75 | 4.89 | 6.44 | 5.84 | 5.56 |
Endoplasmic Reticulum | -3.83 | -7.64 | -6.85 | -3.48 | -1.76 | 3.15 | -2.42 | 2.25 | -4.69 | 5.86 | 2.30 | -1.64 | 0.07 | -5.60 | 3.19 |
Endosome | 1.34 | 2.93 | 2.61 | 5.55 | -2.48 | 1.50 | 2.77 | 1.71 | 0.39 | -2.37 | 1.98 | 3.76 | 3.02 | 3.86 | -3.71 |
Golgi | 0.33 | 2.20 | 2.30 | 8.11 | 1.24 | -0.82 | 4.86 | 3.18 | 5.14 | -3.67 | 0.31 | 2.95 | 2.90 | 9.34 | -0.39 |
Lipid Particles | 0.75 | 1.22 | 1.10 | 1.05 | -0.53 | 4.02 | 1.26 | 4.14 | 0.50 | 4.65 | 2.98 | 2.50 | 3.26 | 1.00 | 2.44 |
Mitochondria | 0.06 | 2.71 | 2.68 | 7.22 | -0.23 | -0.67 | 1.99 | 1.67 | 6.15 | -2.22 | -0.25 | 3.30 | 3.13 | 9.34 | -1.28 |
Mitotic Spindle | -1.64 | -4.50 | -4.63 | -4.04 | -0.68 | -0.74 | -2.10 | -4.88 | -5.29 | -1.99 | -1.36 | -4.73 | -6.71 | -6.64 | -1.88 |
None | 0.90 | 0.59 | 0.93 | 0.42 | 2.53 | 0.24 | 1.58 | 2.99 | 0.90 | 1.12 | 0.89 | 0.57 | 1.21 | 0.94 | 2.98 |
Nuclear Periphery | -0.24 | -6.24 | -7.06 | -7.19 | -2.24 | 2.11 | -3.35 | -0.25 | -3.98 | 3.56 | 2.05 | -4.21 | -3.28 | -7.56 | 1.07 |
Nuclear Periphery Foci | -1.37 | -4.57 | -4.21 | -3.42 | -2.51 | 3.25 | -2.13 | 1.66 | -4.19 | 4.07 | 2.83 | -1.03 | -0.85 | -5.16 | 0.16 |
Nucleolus | -1.52 | -0.42 | -0.67 | 1.42 | -0.41 | 1.31 | 1.14 | 1.16 | -3.39 | 0.44 | 0.74 | 1.12 | 1.11 | 0.91 | -0.08 |
Nucleus | 1.36 | -6.44 | -3.55 | -6.25 | 3.04 | 3.91 | -2.27 | 0.52 | -8.15 | 3.32 | 4.09 | -4.48 | -1.40 | -10.24 | 4.30 |
Peroxisomes | -2.14 | 0.22 | -2.53 | -0.47 | -4.17 | 2.63 | 1.97 | 2.15 | -2.15 | 0.26 | 1.85 | 2.27 | 1.17 | -2.06 | -3.18 |
Vacuole | -1.61 | -6.64 | -7.18 | -6.89 | -2.14 | 1.99 | -8.33 | -5.83 | -11.81 | 5.63 | 1.00 | -9.29 | -9.17 | -13.54 | 0.66 |
Vacuole Periphery | -1.51 | 2.63 | 2.16 | 3.78 | -0.92 | 0.45 | 2.74 | 1.38 | 1.29 | -1.36 | -0.66 | 3.73 | 2.38 | 3.56 | -1.48 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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