Standard name
Human Ortholog
Description ATP-binding protein required for mismatch repair; required for both mitosis and meiosis; functions as a heterodimer with Mlh1p; binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – – – – – – – – – – – – – 0 0 0 0 0 0 0 0 0
Bud 0 0 – – – – – – – – – – – – – 0 0 0 0.05 0 0 0 0 0
Bud Neck 0 0 – – – – – – – – – – – – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – – – – – – – – – – – – – 0 0 0 – – – – – –
Cell Periphery 0 0 – – – – – – – – – – – – – 0 0 0 0 0 0 0 0 0
Cytoplasm 0.41 0.46 – – – – – – – – – – – – – 0.34 0.24 0.3 0.22 0.28 0.3 0.28 0.21 0.16
Endoplasmic Reticulum 0 0 – – – – – – – – – – – – – 0.05 0.06 0.07 0 0 0 0 0 0
Endosome 0 0 – – – – – – – – – – – – – 0 0 0 0 0 0 0 0 0
Golgi 0 0 – – – – – – – – – – – – – 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0.13 – – – – – – – – – – – – – 0 0 0 0 0 0 0.11 0.09 0.05
Nucleus 0.42 0.31 – – – – – – – – – – – – – 0.5 0.52 0.49 0.43 0.37 0.3 0.16 0.17 0.21
Nuclear Periphery 0 0 – – – – – – – – – – – – – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – – – – – – – – – – – – – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – – – – – – – – – – – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – – – – – – – – – – – – – 0 0 0 0.08 0.06 0.06 0 0 0
Vac/Vac Membrane 0.14 0.18 – – – – – – – – – – – – – 0.18 0.25 0.22 0.14 0.17 0.21 0.33 0.38 0.36
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – – – – – – – – – – – – – 0 0 0 0 0 2 0 2 6
Bud 1 0 – – – – – – – – – – – – – 0 0 0 15 9 2 2 5 7
Bud Neck 1 2 – – – – – – – – – – – – – 0 0 0 2 2 1 2 2 6
Bud Site 0 0 – – – – – – – – – – – – – 0 0 0 – – – – – –
Cell Periphery 1 0 – – – – – – – – – – – – – 1 0 1 0 0 1 0 1 0
Cytoplasm 114 148 – – – – – – – – – – – – – 68 52 64 69 109 65 43 66 45
Endoplasmic Reticulum 6 3 – – – – – – – – – – – – – 10 14 14 1 0 1 1 4 5
Endosome 2 0 – – – – – – – – – – – – – 1 8 5 2 4 2 0 3 4
Golgi 0 1 – – – – – – – – – – – – – 0 2 2 2 0 0 0 0 3
Mitochondria 20 43 – – – – – – – – – – – – – 8 4 5 5 14 8 17 29 14
Nucleus 117 101 – – – – – – – – – – – – – 99 114 102 137 143 66 24 53 58
Nuclear Periphery 0 0 – – – – – – – – – – – – – 0 0 0 2 0 0 0 1 0
Nucleolus 0 1 – – – – – – – – – – – – – 0 0 1 1 0 0 0 3 3
Peroxisomes 0 0 – – – – – – – – – – – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 – – – – – – – – – – – – – 1 1 3 25 25 12 4 12 12
Vac/Vac Membrane 39 58 – – – – – – – – – – – – – 35 56 47 45 68 46 51 118 99
Unique Cell Count 276 323 198 221 210 317 393 221 158 317 279
Labelled Cell Count 301 358 223 251 244 317 393 221 158 317 279


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 3.9 – – – – – – – – – – – – – 6.7 6.9 6.4 – – –
Std Deviation (1e-4) 0.9 0.6 – – – – – – – – – – – – – 1.8 2.1 1.9 – – –
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.7035 2.2834 2.1536 2.0582 1.6478 2.0619 0.0457 -0.0366 0.1156 -0.0766 -0.5452 -0.5555 -0.3784 0.1605 0.5017 -0.3618 -0.5547 -0.2577
Actin 0.0583 0.0006 0.0117 0.0158 0.0149 0.0028 0.0498 0.0002 0.011 0.0536 0.0175 0.003 0.0017 0.0006 0.0005 0.0005 0.0006 0.0012
Bud 0.0032 0.0002 0.0006 0.0001 0.0085 0.0002 0.0023 0.0002 0.0008 0.007 0.0005 0.0005 0.0097 0.0108 0.0038 0.0145 0.0046 0.0086
Bud Neck 0.0104 0.0019 0.0024 0.0004 0.0014 0.0037 0.0038 0.0006 0.0016 0.0075 0.0024 0.0039 0.0077 0.002 0.0016 0.002 0.0117 0.0158
Bud Periphery 0.0067 0.0002 0.0007 0.0004 0.0129 0.0006 0.0037 0.0002 0.0009 0.0129 0.0009 0.0005 0.0051 0.0033 0.0012 0.0057 0.0025 0.0064
Bud Site 0.0123 0.015 0.0079 0.0002 0.0092 0.0003 0.0068 0.003 0.0068 0.0045 0.0011 0.0002 0.0222 0.022 0.0045 0.0029 0.0034 0.0029
Cell Periphery 0.0012 0.0002 0.0004 0 0.0003 0.0001 0.0005 0.0001 0.0002 0.0005 0.0002 0.0001 0.0009 0.0003 0.0001 0.0002 0.0005 0.0002
Cytoplasm 0.1076 0.0536 0.0338 0.0399 0.072 0.084 0.1289 0.1062 0.0456 0.1248 0.1046 0.1981 0.0667 0.0315 0.023 0.0315 0.0642 0.0747
Cytoplasmic Foci 0.0165 0.0047 0.0059 0.0366 0.0221 0.0077 0.0432 0.0063 0.0102 0.0148 0.0105 0.0108 0.0247 0.0023 0.0036 0.0088 0.0145 0.0107
Eisosomes 0.0009 0.0001 0.0008 0.0002 0.0004 0.0002 0.0005 0 0.0001 0.0007 0.0003 0.0001 0.0002 0.0001 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0098 0.0023 0.0013 0.0008 0.0039 0.0016 0.0081 0.0038 0.0026 0.0064 0.0076 0.0037 0.003 0.0005 0.0006 0.0009 0.0016 0.0012
Endosome 0.0246 0.0026 0.0024 0.0016 0.0269 0.0047 0.0471 0.0042 0.006 0.0153 0.0243 0.0058 0.0018 0.0001 0.0011 0.0004 0.0006 0.0004
Golgi 0.0068 0.0004 0.0024 0.0021 0.0091 0.0012 0.0191 0.0001 0.0035 0.0092 0.0149 0.004 0.0004 0 0.0002 0.0001 0.0001 0.0001
Lipid Particles 0.0105 0.0008 0.0038 0.0087 0.0195 0.0078 0.0253 0.0019 0.0032 0.0111 0.0288 0.0027 0.0018 0.0001 0.0018 0.0002 0.0005 0.0002
Mitochondria 0.0091 0.0006 0.0123 0.0012 0.0463 0.0022 0.0143 0.0007 0.0116 0.0301 0.009 0.0018 0.0042 0.0003 0.0014 0.001 0.0005 0.0011
None 0.4342 0.3452 0.2217 0.592 0.6084 0.5807 0.3602 0.2514 0.1122 0.3066 0.543 0.3903 0.175 0.0519 0.0677 0.1918 0.2272 0.1273
Nuclear Periphery 0.022 0.01 0.0172 0.009 0.0138 0.0067 0.0308 0.0199 0.0134 0.0186 0.0162 0.0132 0.0107 0.0058 0.0076 0.0114 0.0084 0.0101
Nucleolus 0.0081 0.0098 0.0091 0.0034 0.0045 0.0092 0.007 0.0048 0.0049 0.0033 0.0021 0.0041 0.0256 0.0099 0.013 0.0121 0.0192 0.0115
Nucleus 0.2112 0.5091 0.6225 0.1944 0.0718 0.2484 0.1767 0.5515 0.7301 0.3409 0.1896 0.3124 0.2964 0.3734 0.4253 0.2259 0.2306 0.3248
Peroxisomes 0.0169 0.0005 0.0171 0.0264 0.0256 0.003 0.0201 0.0002 0.0105 0.0033 0.0104 0.0077 0.001 0.0001 0.0004 0.0002 0.0002 0.0003
Punctate Nuclear 0.016 0.0401 0.0246 0.0659 0.0253 0.0332 0.0394 0.041 0.0217 0.021 0.0116 0.0326 0.3373 0.4845 0.4415 0.488 0.4064 0.4014
Vacuole 0.0109 0.0018 0.0009 0.0005 0.0021 0.0013 0.0096 0.0031 0.0024 0.0055 0.0029 0.0037 0.0035 0.0005 0.0007 0.0013 0.0025 0.0009
Vacuole Periphery 0.0028 0.0002 0.0004 0.0003 0.001 0.0004 0.0029 0.0006 0.0006 0.0024 0.0016 0.0008 0.0006 0.0001 0.0002 0.0004 0.0002 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 20.8309 6.0191 5.8622 13.4406 17.4598 10.5148 5.8006 5.0061 9.5145 18.9725
Translational Efficiency 0.9342 0.6404 0.8082 0.7905 0.7239 2.1155 0.8096 0.6658 0.9828 0.8961

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1949 1666 244 1605 1510 1977 179 898 3459 3643 423 2503

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 663.62 743.36 893.10 870.88 617.96 724.43 870.09 816.80 643.69 733.09 883.36 851.48
Standard Deviation 70.13 100.04 107.21 121.67 74.40 101.43 106.05 140.90 75.50 101.24 107.32 131.48
Intensity Change Log 2 — 0.163704 0.428464 0.392117 — 0.229333 0.493651 0.402469 — 0.195722 0.460264 0.397118

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000434 0.000185 0.000720 0.000771 0.000102 0.000243 0.000403 0.001413 0.000289 0.000216 0.000586 0.001002
Bud Neck 0.005347 0.013815 0.003309 0.023160 0.006948 0.025783 0.001446 0.018796 0.006046 0.020310 0.002520 0.021594
Bud Site 0.003234 0.003935 0.004321 0.019695 0.001611 0.007336 0.000483 0.020928 0.002525 0.005780 0.002697 0.020138
Cell Periphery 0.000247 0.000142 0.000134 0.000248 0.000214 0.000245 0.000067 0.000389 0.000233 0.000198 0.000106 0.000299
Cytoplasm 0.287075* 0.212950* 0.021850 0.045273 0.295703* 0.234580* 0.013786 0.033821 0.290841* 0.224688* 0.018437 0.041165
Cytoplasmic Foci 0.031833 0.043512 0.002875 0.001405 0.080571 0.067052 0.001312 0.001759 0.053109 0.056287 0.002213 0.001532
Eisosomes 0.000039 0.000041 0.000083 0.000028 0.000062 0.000068 0.000049 0.000033 0.000049 0.000056 0.000069 0.000030
Endoplasmic Reticulum 0.000512 0.001814 0.009579 0.009331 0.000839 0.001407 0.006385 0.008451 0.000655 0.001593 0.008228 0.009015
Endosome 0.001228 0.003374 0.001538 0.002262 0.001758 0.007532 0.001384 0.001098 0.001460 0.005630 0.001473 0.001845
Golgi 0.000808 0.000769 0.000081 0.001859 0.000456 0.002688 0.000069 0.000152 0.000654 0.001810 0.000076 0.001246
Lipid Particles 0.002281 0.003286 0.002197 0.000339 0.005037 0.005125 0.001063 0.000501 0.003484 0.004284 0.001717 0.000397
Mitochondria 0.001473 0.005052 0.000838 0.003296 0.003564 0.013393 0.000399 0.002584 0.002386 0.009579 0.000652 0.003040
Mitotic Spindle 0.001887 0.003956 0.003128 0.020120 0.000698 0.007669 0.000072 0.017246 0.001368 0.005971 0.001835 0.019089
None 0.014410 0.007968 0.005921 0.006123 0.019514 0.009337 0.003128 0.019281 0.016638 0.008711 0.004739 0.010844
Nuclear Periphery 0.000674 0.001392 0.001364 0.004195 0.000956 0.001518 0.001009 0.001174 0.000797 0.001460 0.001214 0.003111
Nuclear Periphery Foci 0.000175 0.000401 0.000574 0.000808 0.000303 0.000284 0.000275 0.000535 0.000231 0.000337 0.000447 0.000710
Nucleolus 0.002703 0.004697 0.002964 0.001704 0.003309 0.006906 0.001456 0.004044 0.002967 0.005896 0.002326 0.002543
Nucleus 0.639275* 0.676829* 0.907774* 0.842046* 0.566294* 0.579119* 0.943124* 0.853452* 0.607415* 0.623804* 0.922733* 0.846138*
Peroxisomes 0.001400 0.003527 0.000429 0.000321 0.003794 0.007634 0.000173 0.000320 0.002445 0.005756 0.000321 0.000320
Vacuole 0.004779 0.011612 0.029933 0.016273 0.007925 0.020372 0.021413 0.012980 0.006153 0.016366 0.026328 0.015091
Vacuole Periphery 0.000186 0.000745 0.000388 0.000744 0.000343 0.001707 0.002505 0.001042 0.000255 0.001267 0.001284 0.000851

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.59 -1.65 -1.43 -13.94 0.91 -2.83 -5.64 -9.41 -8.54 -4.86 0.28 -2.83 -4.16 -14.03 -3.03
Bud Neck -5.82 0.20 -12.32 -6.25 -12.42 -9.60 5.24 -6.63 1.62 -9.29 -11.40 3.12 -13.84 -2.96 -15.66
Bud Site -0.67 -0.84 -5.06 -4.99 -3.46 -6.16 1.11 -5.94 -3.92 -6.08 -4.18 -0.66 -7.95 -5.84 -6.76
Cell Periphery 4.49 3.41 2.40 -1.35 -0.64 -0.96 6.14 -1.51 -1.00 -3.89 1.84 6.02 0.45 -0.84 -3.58
Cytoplasm 8.64 34.59 38.34 27.80 -2.57 6.74 37.64 35.39 32.97 -3.84 10.68 50.04 52.11 43.18 -4.24
Cytoplasmic Foci -3.23 14.07 16.26 16.34 2.89 3.01 20.92 20.47 22.23 -0.49 -0.78 24.69 25.67 27.87 2.21
Eisosomes -1.10 -7.29 -0.39 0.88 7.29 -1.55 -0.78 2.20 3.72 1.89 -2.83 -6.47 1.82 4.91 7.24
Endoplasmic Reticulum -9.32 -12.39 -20.79 -18.00 1.60 -4.62 -8.66 -15.73 -14.92 -1.41 -10.22 -15.02 -26.02 -23.63 0.40
Endosome -3.81 -1.52 -0.25 3.67 1.31 -8.00 0.87 1.94 8.77 0.03 -8.77 -0.53 0.51 9.04 0.93
Golgi 0.13 1.98 0.11 0.01 -1.62 -3.54 4.58 3.30 4.02 -2.80 -2.79 2.76 0.33 2.85 -1.76
Lipid Particles -2.61 -1.01 6.72 9.34 5.74 -0.00 7.01 8.38 11.40 2.85 -2.10 3.86 10.72 14.85 6.37
Mitochondria -4.59 1.95 -4.46 2.80 -8.89 -6.18 3.84 1.05 7.64 -8.68 -7.93 4.28 -1.12 8.04 -12.52
Mitotic Spindle -1.74 -1.19 -7.16 -6.28 -3.57 -5.23 0.37 -5.47 -3.22 -5.52 -4.87 -1.16 -9.06 -6.68 -5.80
None 5.17 6.38 7.78 2.82 -1.10 5.58 9.33 2.74 -1.75 -5.26 7.46 10.87 7.18 0.55 -4.55
Nuclear Periphery -7.80 -6.70 -8.84 -7.18 -5.66 -3.24 -2.33 -7.28 -4.42 -5.51 -6.91 -6.81 -10.14 -7.86 -6.84
Nuclear Periphery Foci -3.26 -2.40 -5.23 -3.76 0.73 0.67 -1.38 -2.82 -2.94 -2.15 -2.00 -2.67 -5.09 -4.66 -0.52
Nucleolus -2.50 0.18 -1.78 0.41 -1.90 -3.84 3.37 -1.70 1.13 -4.27 -4.75 1.97 -2.38 1.50 -4.10
Nucleus -3.52 -17.33 -21.29 -16.56 3.34 -1.14 -27.73 -22.87 -22.94 6.86 -2.10 -29.80 -31.41 -29.30 6.68
Peroxisomes -3.31 3.44 4.19 6.89 3.13 -4.04 5.65 5.47 10.28 -1.70 -5.76 6.59 6.89 12.35 1.87
Vacuole -8.82 -6.10 -11.44 -7.49 2.31 -9.57 -4.07 -7.27 -3.09 0.44 -13.12 -7.32 -13.28 -6.76 2.14
Vacuole Periphery -4.43 -1.81 -2.42 2.44 -0.22 -5.37 -0.83 -0.85 2.13 0.54 -6.78 -1.00 -1.54 3.61 0.55
Standard name
Human Ortholog
Description ATP-binding protein required for mismatch repair; required for both mitosis and meiosis; functions as a heterodimer with Mlh1p; binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL
Localization
Cell Percentages nucleus (48%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Pms1

Pms1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pms1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available