Standard name
Human Ortholog
Description Protein similar to nucleases that forms a complex with Pbp1p; complex may mediate posttranscriptional regulation of HO; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability, drug resistance, and more; forms cytoplasmic foci upon DNA replication stress; localization to P-bodies under ethanol stress differs between strains

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0.06 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.96 0.93 0.96 0.96 0.89 0.88 0.9 0.81 0.75 0.7 0.78 0.95 0.98 0.97 0.77 0.8 0.93 0.92 0.92 0.82 0.66 0.65
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0 0 0 0.05 0 0.05
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.15 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.05 0 0.18 0.2 0.11 0.4 0.45 0.43 0.29 0 0 0 0.09 0 0 0 0 0 0.07 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.05 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.05 0 0 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 1 0 0 1 0 0 1 0 0 1 1 2 5 5
Bud 1 4 0 0 0 4 4 1 5 7 10 2 5 2 1 0 0 0 3 0 1 16 10
Bud Neck 0 0 0 0 0 0 1 1 1 1 0 0 0 0 0 0 0 0 2 1 0 3 2
Bud Site 0 0 0 0 0 0 1 0 1 0 1 0 0 0 0 0 0
Cell Periphery 2 11 12 5 3 3 8 3 7 3 1 4 4 7 1 1 0 0 0 0 0 0 1
Cytoplasm 113 505 244 126 211 190 237 284 204 180 154 187 227 367 368 27 16 112 504 237 143 196 169
Endoplasmic Reticulum 1 10 2 0 0 0 0 0 0 0 0 3 0 0 2 1 2 0 2 2 8 12 12
Endosome 0 1 0 0 3 2 5 2 0 3 4 2 3 5 4 1 3 1 4 0 0 10 10
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 4 0 1 6 6
Mitochondria 2 6 5 6 9 38 53 36 101 107 95 69 6 4 3 3 0 1 8 3 6 20 12
Nucleus 0 2 1 1 2 0 3 9 2 7 3 7 0 1 0 0 0 0 1 1 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 0 1 0 1 2 1 0 1 0 0 1 0 2 0 1 3 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 2 5 5 0 0 0 0 0 1 7 7
SpindlePole 0 1 0 1 2 0 1 3 0 4 4 4 1 3 3 0 2 0 1 0 0 4 4
Vac/Vac Membrane 0 3 16 3 2 6 5 4 4 1 1 1 1 0 1 3 1 0 10 4 5 10 15
Unique Cell Count 116 525 262 131 220 214 269 315 253 239 221 241 239 376 379 35 20 121 549 259 175 299 262
Labelled Cell Count 119 545 281 143 234 244 318 345 325 314 274 279 252 394 389 41 25 121 549 259 175 299 262


Cytoplasm, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.1 7.2 7.3 6.8 6.9 4.8 5.4 5.7 4.4 4.3 4.3 4.4 7.6 6.1 5.5 9.4 7.8 7.0 6.0 6.2
Std Deviation (1e-4) 1.2 1.4 1.1 1.6 2.1 1.2 1.9 2.0 1.4 1.5 1.9 1.3 1.9 1.1 1.5 2.6 2.2 1.7 1.5 1.7
Intensity Change (Log2) -0.11 -0.09 -0.61 -0.44 -0.36 -0.74 -0.76 -0.77 -0.72 0.06 -0.26 -0.41 0.36 0.1 -0.06 -0.29 -0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.4 -2.0 0 -1.0 -2.7 0 0 0 0 -1.8 -2.0 -3.8 0 0
Cytoplasm 1.2 1.3 -1.7 -2.0 -1.3 -4.2 -5.5 -6.7 -5.0 0.9 2.8 2.4 -3.1 -2.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 1.4 0 6.6 4.4 0 0 0 0 0.5 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.7 -3.0 -1.7 -2.5 -3.2 -2.7 -3.5 -3.4 -3.5 -3.5 -4.9 -4.5 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.4655 6.6952 5.8557 4.9203 4.7998 5.7004 2.6796 4.37 3.6928 2.9074 3.3296 3.8257 2.0128 3.1859 2.8728 3.0406 2.7504 2.9792
Actin 0.0202 0.0004 0.0029 0.0003 0.0351 0.0064 0.0085 0.0007 0.0079 0.0107 0.0013 0.0063 0.0018 0.0001 0.0019 0.0002 0.0195 0.0016
Bud 0.0013 0.0002 0.0004 0.0004 0.0003 0.0003 0.0005 0.0028 0.001 0.0015 0.0003 0.0008 0.0002 0.0002 0.001 0.0003 0.0003 0.0001
Bud Neck 0.0037 0.0003 0.0005 0.0004 0.0009 0.0012 0.0006 0.0002 0.0018 0.0067 0.0004 0.0012 0.0002 0.0001 0.0008 0.0002 0.0003 0.0005
Bud Periphery 0.0015 0.0001 0.0004 0.0002 0.0004 0.0002 0.0003 0.0018 0.0008 0.0012 0.0003 0.0012 0.0001 0.0001 0.0006 0.0001 0.0002 0.0001
Bud Site 0.0042 0.0042 0.0014 0.0002 0.0014 0.0002 0.0015 0.019 0.0055 0.0035 0.0004 0.0007 0.0008 0.0006 0.0084 0.0001 0.0006 0.0001
Cell Periphery 0.0012 0.0005 0.0003 0.0004 0.0002 0.0002 0.0002 0.0002 0.0002 0.0004 0.0001 0.0001 0.0002 0.0001 0.0002 0 0 0
Cytoplasm 0.8047 0.9022 0.9214 0.9365 0.8335 0.8957 0.8325 0.9263 0.9192 0.6702 0.8905 0.8935 0.8222 0.9317 0.8726 0.9351 0.8979 0.943
Cytoplasmic Foci 0.0248 0.0042 0.0114 0.0052 0.0117 0.0181 0.0239 0.0073 0.0117 0.0576 0.0135 0.0191 0.0389 0.0048 0.0232 0.0077 0.0174 0.0136
Eisosomes 0.0002 0 0.0001 0.0001 0.0001 0.0003 0.0001 0 0.0001 0.0001 0 0.0001 0.0001 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0102 0.002 0.0051 0.0028 0.0017 0.0063 0.0088 0.0011 0.007 0.0075 0.0044 0.0044 0.0056 0.0014 0.0029 0.004 0.0026 0.0034
Endosome 0.0235 0.0025 0.0112 0.0052 0.0146 0.0127 0.0329 0.0133 0.0147 0.089 0.0369 0.031 0.0245 0.0024 0.0226 0.0088 0.0208 0.0129
Golgi 0.0053 0.0003 0.0015 0.0003 0.0049 0.0055 0.0069 0.0009 0.003 0.0349 0.0062 0.0098 0.0042 0.0002 0.005 0.0003 0.0091 0.0014
Lipid Particles 0.005 0.0002 0.0028 0.0013 0.0241 0.0018 0.0084 0.0001 0.0004 0.0067 0.0058 0.0037 0.0056 0 0.004 0.0003 0.0022 0.0008
Mitochondria 0.0035 0.0172 0.0041 0.0003 0.0135 0.0036 0.0103 0.0002 0.0015 0.0435 0.0187 0.0152 0.0072 0.0001 0.0022 0.0003 0.0018 0.0005
None 0.0562 0.0632 0.0288 0.0414 0.0187 0.0383 0.0462 0.0224 0.0176 0.0058 0.0131 0.0061 0.0729 0.0548 0.0445 0.0352 0.0204 0.0184
Nuclear Periphery 0.0015 0.0003 0.0008 0.0006 0.0007 0.0012 0.0041 0.0002 0.0011 0.002 0.001 0.0007 0.0013 0.0005 0.001 0.0024 0.0006 0.0008
Nucleolus 0.0041 0 0.0001 0.0001 0.0008 0.0003 0.0003 0.0001 0.0001 0.0011 0.0002 0.0002 0.0001 0 0.0008 0.0001 0 0
Nucleus 0.0055 0.0006 0.001 0.001 0.0021 0.0009 0.0019 0.0007 0.0015 0.0029 0.0012 0.0009 0.001 0.0013 0.0016 0.0016 0.001 0.0008
Peroxisomes 0.011 0 0.0015 0.0001 0.0274 0.0016 0.0044 0.0001 0.0009 0.0123 0.0008 0.0017 0.008 0 0.002 0.0001 0.0022 0.0003
Punctate Nuclear 0.001 0.0001 0.0005 0.0002 0.0019 0.0037 0.0016 0.0001 0.0004 0.0009 0.0002 0.0005 0.0023 0.0002 0.0008 0.0007 0.0004 0.0006
Vacuole 0.0101 0.0011 0.0034 0.0026 0.0052 0.0012 0.0048 0.0022 0.0031 0.0379 0.0034 0.002 0.0023 0.0011 0.0032 0.0021 0.002 0.0009
Vacuole Periphery 0.0015 0.0002 0.0004 0.0005 0.0011 0.0004 0.0013 0.0003 0.0006 0.0036 0.0012 0.0009 0.0005 0.0001 0.0006 0.0005 0.0007 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 122.3792 102.8712 99.8225 133.3479 109.8926 71.6617 109.4136 109.7577 97.038 142.4559
Translational Efficiency 0.6101 0.7221 0.7697 0.511 0.6185 1.0503 0.6837 0.5728 0.7319 0.5735

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
210 1580 237 1659 1442 1696 2237 1063 1652 3276 2474 2722

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 834.56 950.12 1274.20 1442.10 760.45 898.30 1274.06 1374.50 769.87 923.29 1274.07 1415.70
Standard Deviation 117.32 141.82 195.50 230.56 131.26 132.94 185.47 257.71 131.90 139.71 186.45 243.77
Intensity Change Log 2 0.187094 0.610504 0.789084 0.240344 0.744508 0.853982 0.212727 0.675947 0.820389

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000432 0.000282 0.000632 0.000986 0.000210 0.000239 0.000134 0.003467 0.000238 0.000260 0.000181 0.001955
Bud Neck 0.012805 0.006022 0.005169 0.006588 0.006521 0.004784 0.002530 0.009769 0.007320 0.005381 0.002783 0.007830
Bud Site 0.039090 0.018115 0.019766 0.048353 0.013078 0.022982 0.008744 0.056288 0.016385 0.020634 0.009800 0.051452
Cell Periphery 0.000229 0.000072 0.000199 0.000219 0.000117 0.000085 0.000057 0.000211 0.000131 0.000079 0.000070 0.000216
Cytoplasm 0.565659* 0.668892* 0.428636* 0.558880* 0.631954* 0.696394* 0.783637* 0.496334* 0.623527* 0.683130* 0.749629* 0.534454*
Cytoplasmic Foci 0.234801* 0.170308 0.044445 0.105370 0.185169* 0.165202 0.026174 0.121864 0.191479* 0.167665 0.027925 0.111811
Eisosomes 0.000123 0.000027 0.000062 0.000012 0.000057 0.000029 0.000008 0.000019 0.000066 0.000028 0.000013 0.000015
Endoplasmic Reticulum 0.000707 0.001156 0.002206 0.000213 0.002353 0.000619 0.000439 0.000514 0.002143 0.000878 0.000608 0.000330
Endosome 0.022959 0.013942 0.014199 0.004960 0.014484 0.013451 0.003502 0.010202 0.015561 0.013688 0.004527 0.007007
Golgi 0.008064 0.004197 0.000507 0.004717 0.004351 0.008181 0.001145 0.006005 0.004823 0.006260 0.001084 0.005220
Lipid Particles 0.002530 0.001202 0.004077 0.003621 0.002836 0.001158 0.000687 0.007142 0.002797 0.001179 0.001011 0.004996
Mitochondria 0.007989 0.001456 0.000153 0.000285 0.002321 0.003333 0.000510 0.003191 0.003042 0.002428 0.000476 0.001420
Mitotic Spindle 0.006415 0.005396 0.036495 0.203308* 0.000467 0.003474 0.035926 0.198502* 0.001223 0.004401 0.035981 0.201431*
None 0.000271 0.001106 0.002758 0.001597 0.004051 0.001039 0.002513 0.001265 0.003570 0.001071 0.002536 0.001468
Nuclear Periphery 0.000047 0.000103 0.002556 0.000200 0.000202 0.000043 0.000236 0.000337 0.000182 0.000072 0.000458 0.000253
Nuclear Periphery Foci 0.000403 0.000523 0.010505 0.004792 0.000795 0.000326 0.001963 0.006461 0.000745 0.000421 0.002782 0.005444
Nucleolus 0.000263 0.000212 0.000506 0.000386 0.000766 0.000260 0.000602 0.000966 0.000702 0.000237 0.000593 0.000613
Nucleus 0.009291 0.020729 0.069162 0.018304 0.049818 0.014079 0.047190 0.019509 0.044666 0.017286 0.049295 0.018775
Peroxisomes 0.004450 0.002288 0.002647 0.009346 0.001645 0.002456 0.001518 0.014384 0.002002 0.002375 0.001626 0.011313
Vacuole 0.080758 0.081155 0.354238* 0.026857 0.075595 0.058870 0.081729 0.042168 0.076252 0.069618 0.107834 0.032836
Vacuole Periphery 0.002712 0.002817 0.001081 0.001005 0.003210 0.002997 0.000755 0.001401 0.003147 0.002910 0.000786 0.001160

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.49 -2.22 -1.21 -2.49 0.53 -0.58 0.79 -1.86 -1.82 -1.91 -0.47 0.26 -2.52 -2.46 -2.57
Bud Neck 3.11 3.13 3.18 -0.06 -0.21 2.49 5.56 -0.87 -2.87 -5.14 2.73 6.50 0.98 -1.99 -6.52
Bud Site 4.59 4.89 0.01 -8.40 -7.91 -6.01 3.44 -10.45 -7.06 -12.38 -2.93 5.47 -12.10 -10.69 -17.31
Cell Periphery 6.54 0.88 1.12 -7.09 0.31 2.20 7.74 -5.18 -5.81 -8.38 5.61 7.66 -5.17 -8.65 -9.69
Cytoplasm -5.21 8.54 4.11 16.21 -6.65 -7.06 -7.84 14.20 19.43 20.10 -7.57 -5.11 16.29 25.23 21.44
Cytoplasmic Foci 4.81 15.67 8.22 7.03 -15.77 3.09 32.20 6.91 4.57 -15.11 4.44 35.54 11.18 8.38 -22.86
Eisosomes 5.09 3.58 6.00 8.52 9.38 6.96 13.41 11.23 5.73 -2.59 9.10 13.02 13.33 9.99 1.79
Endoplasmic Reticulum -1.55 -6.16 2.75 4.71 10.04 2.82 3.21 3.39 2.29 1.40 2.34 2.87 3.66 5.40 7.81
Endosome 2.69 3.83 5.96 10.18 6.27 0.89 12.56 7.60 6.30 -4.16 1.82 13.09 11.94 12.13 -2.10
Golgi 1.74 3.46 2.62 3.29 -3.47 -5.41 9.10 2.35 6.18 -2.69 -2.55 9.18 4.37 7.28 -3.74
Lipid Particles 2.03 -1.53 -3.71 -8.88 -2.93 5.19 6.45 -6.03 -7.81 -7.91 6.48 5.62 -7.61 -11.50 -11.05
Mitochondria 1.25 1.48 1.49 2.84 1.56 -1.09 4.03 1.11 1.79 -0.90 0.69 3.41 2.85 3.16 -0.66
Mitotic Spindle 0.23 -2.90 -24.49 -27.54* -18.27 -2.93 -14.03 -21.63 -21.21 -15.32 -3.16 -14.18 -35.47 -34.83* -24.40
None -2.88 -5.57 -10.68 -1.90 1.57 3.86 2.28 3.56 -1.35 4.10 3.65 1.91 3.04 -2.59 3.57
Nuclear Periphery -3.74 -4.05 -17.06 -12.59 3.40 6.58 -8.35 -7.68 -15.78 1.17 4.95 -7.89 -9.89 -20.57 4.02
Nuclear Periphery Foci -1.83 -6.21 -11.55 -11.33 1.90 4.68 -7.06 -11.13 -11.89 -7.68 3.77 -9.18 -15.37 -16.31 -7.65
Nucleolus 0.39 -1.24 -1.49 -4.04 0.58 3.94 1.25 -0.19 -1.77 -0.84 4.69 0.90 0.42 -2.94 -0.44
Nucleus -7.16 -9.43 -7.26 -0.18 8.15 12.96 -2.94 9.40 -4.83 17.18 11.36 -6.18 9.44 -3.93 21.37
Peroxisomes 2.48 2.93 -0.28 -3.14 -3.42 -2.66 1.98 -4.91 -4.07 -5.43 -1.42 2.98 -5.49 -4.98 -6.71
Vacuole -0.46 -16.81 2.65 6.08 18.81 4.70 -11.07 -2.42 -5.80 6.69 1.73 -15.98 1.52 -0.03 17.25
Vacuole Periphery -0.09 3.21 3.35 5.27 0.44 0.33 5.09 4.74 6.25 -0.71 0.47 5.58 5.40 8.04 -0.24
Standard name
Human Ortholog
Description Protein similar to nucleases that forms a complex with Pbp1p; complex may mediate posttranscriptional regulation of HO; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability, drug resistance, and more; forms cytoplasmic foci upon DNA replication stress; localization to P-bodies under ethanol stress differs between strains
Localization
Cell Percentages cytoplasm (94%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Mkt1

Mkt1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mkt1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available