Standard name
Human Ortholog
Description ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains 3 calcium and lipid binding domains; mRNA is targeted to bud; TCB2 has a paralog, TCB1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.25 0.32 0.43 0.35 0.41 0.51 0.4 0.34 – 0.33 0.41 0.41 0.36 0.29 0.21 0.3 0.35 0.28 0 0.07 0.09 0.12 0.06 0.05
Bud 0 0.05 0.05 0 0 0 0 0 – 0 0.09 0 0 0 0 0 0.08 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.77 0.72 0.63 0.61 0.54 0.47 0.32 0.25 – 0.35 0.34 0.43 0.82 0.89 0.85 0.7 0.53 0.68 0.91 0.81 0.82 0.76 0.86 0.9
Cytoplasm 0 0.08 0 0.05 0.05 0 0 0 – 0 0 0 0.07 0.08 0.1 0.11 0.17 0.14 0 0 0 0 0 0
Endoplasmic Reticulum 0.11 0.08 0.13 0.12 0.2 0.23 0.26 0.29 – 0.22 0.23 0.16 0.08 0 0.07 0 0.05 0.05 0 0 0.05 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.26 0.35 0.55 0.65 0.48 0.61 0.6 0.65 – 0.73 0.74 0.74 0 0 0 0.09 0.1 0.12 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 111 74 157 94 107 175 214 142 – 147 153 182 62 49 38 198 168 117 21 18 35 31 20 19
Bud 12 11 20 11 7 12 24 15 – 16 32 18 3 1 2 29 37 10 2 3 1 3 2 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 2 2 3 2 2 2
Bud Site 0 0 0 1 0 1 0 0 – 1 0 0 0 0 0 0 1 0 – – – – – –
Cell Periphery 338 166 233 166 140 161 170 107 – 153 125 192 142 151 151 465 257 278 468 217 333 203 293 341
Cytoplasm 10 18 13 13 13 11 15 16 – 11 6 6 12 13 18 74 82 56 1 2 1 0 1 1
Endoplasmic Reticulum 47 18 48 34 51 78 137 120 – 96 86 72 14 6 12 27 23 21 5 10 18 11 7 6
Endosome 0 0 0 0 0 1 0 0 – 0 0 0 0 0 0 5 2 1 2 2 1 1 1 0
Golgi 3 1 4 8 5 0 16 5 – 6 0 1 1 0 0 17 16 14 1 1 1 0 1 0
Mitochondria 115 81 204 176 125 210 323 275 – 323 274 329 5 4 5 58 49 48 5 3 4 11 7 5
Nucleus 2 1 0 1 1 0 2 2 – 2 0 0 0 0 1 7 1 3 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 2 1 2 3 – 10 2 7 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 1 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 10 2 6 8 17 8 – 10 3 10 1 0 0 12 0 1 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 0 0 0 – 0 0 0 0 0 0 3 2 2 0 0 0 0 0 0
Vac/Vac Membrane 3 0 1 0 0 1 0 1 – 0 0 2 1 1 0 19 2 2 1 3 1 0 1 0
Unique Cell Count 440 230 368 272 260 342 536 420 443 370 446 174 170 178 666 483 411 513 269 408 268 341 381
Labelled Cell Count 642 370 691 506 457 659 920 694 775 681 819 241 225 227 914 640 554 513 269 408 268 341 381


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 15.5 14.8 12.7 13.0 13.6 12.4 12.3 11.8 – 11.2 10.7 11.3 13.5 13.3 13.1 10.2 11.4 13.5 14.2 15.0 15.0
Std Deviation (1e-4) 2.1 2.9 2.2 2.1 2.5 2.2 2.2 2.1 – 2.2 2.2 2.2 2.3 1.9 2.3 1.9 2.4 2.9 2.3 2.3 2.3
Intensity Change (Log2) – – – 0.04 0.1 -0.04 -0.05 -0.1 – -0.17 -0.24 -0.16 0.09 0.07 0.05 -0.32 -0.16 0.09 0.17 0.24 0.24

WT3RAP60RAP140RAP220RAP300RAP380RAP540RAP620RAP700051015WT3HU80HU120HU160051015WT3rpd3Δ_1rpd3Δ_2rpd3Δ_3051015WT1AF100AF140AF180051015
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.1 -0.4 2.3 -0.8 -2.6 – -2.8 -0.4 -0.5 -1.6 -3.1 -4.9 -4.2 -2.3 -4.1
Bud -0.8 -1.7 -1.2 -0.7 -1.3 – -1.3 1.7 -0.9 -2.0 -2.7 -2.4 -0.8 1.3 -2.2
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery -0.6 -2.4 -4.4 -9.4 -10.7 – -8.2 -8.0 -5.8 4.3 6.1 5.2 2.1 -3.0 1.3
Cytoplasm 0.8 0.9 -0.2 -0.6 0.2 – -0.9 -1.6 -2.1 1.7 2.1 3.1 4.2 6.2 4.9
Endoplasmic Reticulum -0.2 2.2 3.4 4.6 5.3 – 3.2 3.6 1.2 -1.7 -3.4 -2.2 -5.3 -4.3 -3.9
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 1.7 0 0 1.9 0 – 0 0 0 0 0 0 1.6 2.1 2.2
Mitochondria 2.4 -1.8 1.6 1.4 2.9 – 5.2 5.3 5.5 -11.7 -11.8 -11.9 -16.5 -14.3 -13.0
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes -1.8 -0.3 -0.3 0.4 -0.8 – -0.4 -2.0 -0.4 0 0 0 -1.0 0 -2.9
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 – 0 0 0 0 0 0 2.9 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0094 0.0083 0.0315 0.0261 0.0347 0.004 0.0455 0.0014 0.0273 0.0905 0.0236 0.0045 0.034 0.0019 0.148 0 0.0026 0.0215
Bud 0.0007 0.0011 0.0056 0.0032 0.0016 0.0013 0.0045 0.001 0.0027 0.0009 0.0013 0.0011 0.0475 0.0004 0.0047 0 0.0003 0.0009
Bud Neck 0.0046 0.0126 0.0137 0.0105 0.0034 0.0321 0.0049 0.0035 0.0114 0.011 0.0027 0.0213 0.0019 0.0098 0.0127 0.0006 0.0191 0.1049
Bud Periphery 0.0054 0.004 0.0711 0.0438 0.0216 0.0203 0.0252 0.0103 0.0296 0.0103 0.0132 0.014 0.0247 0.0022 0.0641 0.001 0.0048 0.0146
Bud Site 0.0159 0.2212 0.0576 0.0129 0.0031 0.0038 0.0157 0.1416 0.0308 0.0058 0.0051 0.0021 0.0772 0.3957 0.0197 0.0003 0.0055 0.0048
Cell Periphery 0.6252 0.5235 0.6517 0.5564 0.7534 0.8529 0.6799 0.7674 0.7909 0.7186 0.826 0.8945 0.0011 0.5482 0.6887 0.9552 0.7235 0.749
Cytoplasm 0.0352 0.1387 0.0475 0.0237 0.0127 0.0159 0.0103 0.0164 0.0114 0.0071 0.003 0.0056 0.1662 0.0324 0.0064 0.0012 0.0087 0.0109
Cytoplasmic Foci 0.0091 0.0062 0.0129 0.0704 0.0477 0.0038 0.0096 0.0001 0.0041 0.0094 0.0095 0.0013 0.0451 0.0001 0.0009 0 0.0001 0.0001
Eisosomes 0.0325 0.0166 0.0374 0.0382 0.0038 0.0095 0.0909 0.0473 0.0422 0.0254 0.0662 0.0359 0.0003 0.0037 0.0394 0.0255 0.2274 0.0867
Endoplasmic Reticulum 0.0295 0.0103 0.0137 0.0132 0.0017 0.0088 0.0219 0.0038 0.013 0.0102 0.0082 0.0084 0.0052 0.0006 0.0029 0.0099 0.0036 0.0015
Endosome 0.0954 0.026 0.0162 0.0616 0.0162 0.008 0.0157 0 0.006 0.0079 0.0013 0.0002 0.0206 0 0.0007 0 0 0
Golgi 0.0027 0.0045 0.0093 0.0227 0.0439 0.0105 0.0075 0.0001 0.0065 0.015 0.0023 0.0005 0.0046 0 0.003 0 0.0005 0.0003
Lipid Particles 0.0047 0.005 0.0051 0.0193 0.0055 0.0008 0.0078 0.0002 0.0067 0.023 0.0029 0.0019 0.0021 0.0002 0.0005 0.0001 0.0003 0.0001
Mitochondria 0.003 0.0006 0.0038 0.0559 0.0046 0.016 0.0208 0.0005 0.0027 0.0274 0.0068 0.001 0.0401 0 0.0024 0.0001 0.0005 0.0003
None 0.0118 0.0038 0.0007 0.0023 0.0004 0.0004 0.017 0.0006 0.001 0.0021 0.0006 0.0005 0.0285 0.0003 0.0003 0.0003 0.0006 0.0002
Nuclear Periphery 0.0631 0.0011 0.0017 0.0032 0.0002 0.0012 0.0049 0.0014 0.002 0.0009 0.0007 0.0007 0.0503 0.0003 0.0009 0.001 0.0007 0.0003
Nucleolus 0.0012 0.0005 0.0007 0.0005 0.0001 0.0002 0.0005 0.0001 0.0003 0.0008 0.0002 0.0001 0.0185 0.0001 0.0001 0 0.0001 0.0002
Nucleus 0.0105 0.0009 0.0011 0.0007 0.0001 0.0006 0.0011 0.0003 0.0004 0.0006 0.0003 0.0002 0.3164 0.0003 0.0003 0 0.0001 0.0003
Peroxisomes 0.0014 0.0003 0.0062 0.0106 0.0437 0.0006 0.0079 0 0.0036 0.0278 0.0209 0.0024 0.0103 0 0.0003 0 0.0001 0.0001
Punctate Nuclear 0.0192 0.0002 0.0007 0.0148 0.0004 0 0.0009 0 0.0002 0.0002 0.0001 0 0.0826 0 0.0001 0 0 0
Vacuole 0.0153 0.0132 0.0107 0.0089 0.0012 0.0077 0.0063 0.004 0.0067 0.0046 0.0048 0.0038 0.015 0.0035 0.0036 0.0046 0.0015 0.0033
Vacuole Periphery 0.0042 0.0013 0.001 0.0012 0.0001 0.0016 0.0011 0.0001 0.0005 0.0005 0.0004 0.0002 0.0076 0 0.0002 0.0001 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 55.9969 57.448 37.0416 37.514 44.0873 33.6731 51.3183 46.4356 35.2161 52.0117
Translational Efficiency 0.7202 0.7037 0.7757 0.6787 0.6396 0.9534 0.6914 0.5746 0.6403 0.5513

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1958 920 2058 319 2062 1031 3039 1779 4020 1951 5097 2098

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1198.64 1237.60 1383.30 1302.26 1095.35 1266.01 1399.89 1269.06 1145.66 1252.61 1393.19 1274.11
Standard Deviation 168.05 213.71 203.45 208.42 161.63 168.93 163.08 171.07 172.69 191.88 180.65 177.66
Intensity Change Log 2 — 0.046147 0.206716 0.119619 — 0.208897 0.353922 0.212368 — 0.126151 0.278880 0.164650

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.017879 0.011902 0.004269 0.016081 0.005153 0.005849 0.001784 0.007033 0.011351 0.008703 0.002788 0.008409
Bud Neck 0.007611 0.005819 0.003490 0.009823 0.002111 0.002218 0.001318 0.003633 0.004790 0.003916 0.002195 0.004574
Bud Site 0.033188 0.019287 0.013792 0.050027 0.006716 0.010431 0.002826 0.015922 0.019609 0.014607 0.007254 0.021107
Cell Periphery 0.886229 0.813470 0.918774 0.861680 0.926759 0.945519 0.977325 0.951146 0.907018 0.883251 0.953684 0.937542
Cytoplasm 0.007035 0.004377 0.008343 0.010484 0.001269 0.001147 0.000503 0.003410 0.004077 0.002670 0.003668 0.004486
Cytoplasmic Foci 0.001745 0.004464 0.001734 0.002411 0.000994 0.001328 0.000188 0.000255 0.001360 0.002807 0.000812 0.000583
Eisosomes 0.013326 0.011092 0.004035 0.007834 0.017220 0.010614 0.007597 0.005599 0.015324 0.010839 0.006159 0.005939
Endoplasmic Reticulum 0.014727 0.100280 0.027202 0.022554 0.027897 0.010380 0.004329 0.006134 0.021482 0.052773 0.013565 0.008631
Endosome 0.002987 0.007526 0.002137 0.002931 0.003111 0.002971 0.000781 0.001181 0.003051 0.005119 0.001329 0.001447
Golgi 0.003261 0.003567 0.001380 0.002071 0.000857 0.001135 0.000262 0.000627 0.002028 0.002282 0.000714 0.000847
Lipid Particles 0.000973 0.004720 0.005472 0.003636 0.000455 0.000150 0.000103 0.000344 0.000708 0.002305 0.002271 0.000845
Mitochondria 0.005505 0.003081 0.000702 0.000474 0.002892 0.004924 0.000359 0.000962 0.004165 0.004055 0.000498 0.000888
Mitotic Spindle 0.001013 0.000838 0.000742 0.000869 0.000546 0.000560 0.000337 0.000464 0.000774 0.000691 0.000501 0.000526
None 0.000049 0.000292 0.000048 0.000033 0.000081 0.000035 0.000019 0.000028 0.000066 0.000156 0.000031 0.000029
Nuclear Periphery 0.000311 0.000957 0.001291 0.000554 0.000687 0.000475 0.000566 0.000511 0.000504 0.000703 0.000859 0.000517
Nuclear Periphery Foci 0.000130 0.000309 0.000287 0.000574 0.000068 0.000088 0.000039 0.000088 0.000098 0.000192 0.000139 0.000162
Nucleolus 0.000020 0.000034 0.000038 0.000018 0.000034 0.000019 0.000018 0.000016 0.000027 0.000026 0.000026 0.000016
Nucleus 0.000193 0.000176 0.000225 0.000241 0.000094 0.000066 0.000067 0.000120 0.000142 0.000118 0.000130 0.000138
Peroxisomes 0.001523 0.005250 0.002023 0.000752 0.002211 0.000945 0.000494 0.000226 0.001876 0.002975 0.001111 0.000306
Vacuole 0.001238 0.001240 0.003089 0.004908 0.000308 0.000587 0.000702 0.001508 0.000761 0.000895 0.001666 0.002024
Vacuole Periphery 0.001055 0.001318 0.000926 0.002043 0.000537 0.000559 0.000382 0.000793 0.000789 0.000917 0.000602 0.000983

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.41 8.44 0.44 -0.99 -3.10 -0.50 3.96 -1.33 -0.71 -4.30 1.89 9.55 2.05 0.18 -4.72
Bud Neck 2.37 6.57 -1.85 -3.39 -5.74 -0.31 2.98 -2.94 -2.69 -4.83 2.14 7.86 0.41 -1.29 -5.29
Bud Site 4.32 6.88 -2.35 -4.39 -5.30 -1.74 3.30 -4.20 -2.08 -6.71 2.60 8.35 -0.64 -2.76 -6.87
Cell Periphery 7.26 -5.09 1.80 -3.14 4.32 -3.13 -13.57 -5.01 -0.94 7.23 4.03 -13.10 -6.52 -8.83 4.09
Cytoplasm 2.31 -1.05 -1.91 -3.43 -1.16 0.26 2.27 -3.16 -3.35 -4.91 2.32 0.71 -0.57 -2.61 -1.21
Cytoplasmic Foci -2.80 0.03 -0.49 1.30 -0.51 -0.64 4.28 3.79 2.20 -1.02 -2.70 2.38 2.72 4.14 0.97
Eisosomes 1.76 12.13 4.16 2.15 -3.38 3.96 7.87 9.50 3.96 3.55 4.23 12.41 11.92 5.53 0.48
Endoplasmic Reticulum -13.54 -5.26 -1.68 10.17 0.95 7.58 11.52 10.48 3.03 -2.02 -9.28 5.33 8.66 13.44 3.87
Endosome -7.49 2.27 0.06 4.53 -0.89 0.23 10.20 6.59 2.98 -1.73 -4.80 7.98 5.36 8.30 -0.50
Golgi -0.33 2.25 1.18 2.05 -1.14 -0.68 4.11 0.90 1.12 -1.54 -0.51 3.18 2.58 3.97 -0.60
Lipid Particles -3.76 -5.58 -2.71 0.82 1.56 2.97 3.61 0.71 -1.47 -1.88 -3.35 -4.55 -0.56 3.04 4.10
Mitochondria 2.10 4.40 4.59 6.64 1.78 -1.39 8.68 5.39 2.72 -2.77 0.12 6.63 5.64 3.94 -2.11
Mitotic Spindle 0.41 0.64 0.33 -0.24 -1.01 -0.31 7.58 2.20 2.00 -4.17 0.39 1.31 1.18 3.87 -0.77
None -1.19 0.14 2.83 1.27 2.51 6.33 10.74 8.78 1.49 -3.73 -0.95 8.27 9.00 1.33 0.76
Nuclear Periphery -8.30 -18.43 -2.16 2.98 6.00 4.13 3.02 3.46 -0.63 1.21 -4.33 -11.32 -0.32 3.30 7.56
Nuclear Periphery Foci -6.74 -6.39 -3.12 -1.87 -2.03 -2.75 6.90 -2.72 0.07 -7.19 -6.95 -3.53 -2.62 1.24 -0.99
Nucleolus -6.74 -10.66 0.74 4.53 5.89 6.53 9.99 10.73 1.37 2.24 0.59 0.68 8.77 6.29 9.21
Nucleus 0.94 -1.63 -1.26 -1.84 -0.47 3.37 2.98 -1.10 -2.23 -2.17 2.45 1.19 0.16 -0.97 -0.40
Peroxisomes -3.87 -1.23 2.39 4.81 3.78 2.36 3.72 4.50 2.34 1.88 -2.05 2.54 5.91 5.71 5.38
Vacuole 0.01 -6.43 -3.36 -3.39 -1.66 -2.40 -2.57 -3.22 -2.38 -2.02 -1.06 -5.76 -3.44 -3.10 -0.96
Vacuole Periphery -0.73 0.38 -0.60 -0.45 -0.69 -0.19 1.02 -0.96 -0.82 -1.37 -0.69 1.01 -0.53 -0.20 -1.11
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains 3 calcium and lipid binding domains; mRNA is targeted to bud; TCB2 has a paralog, TCB1, that arose from the whole genome duplication
Localization
Cell Percentages cell periphery (93%)
Cell Cycle Regulation No
Subcompartmental Group cell periphery-2

Tcb2

Tcb2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tcb2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available