ORF
Human Ortholog
Description Putative protein of unknown function; predicted to contain a transmembrane domain; not an essential gene; YNL095C has a paralog, ECM3, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0.08 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.1 0 0 0 0 0 0
Cytoplasm 0.82 0.85 0.74 0.63 0.74 0.72 0.69 0.65 0.61 0.6 0.51 0.57 0.94 0.97 0.99 0.7 0.69 0.62 0.32 0.47 0.13 0.32 0.45 0.52
Endoplasmic Reticulum 0.1 0.09 0.17 0.21 0.1 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0.45 0.25 0.57 0.36 0.24 0.15
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.06 0.08 0.15 0.09 0.33 0.31 0.39 0.48 0.47 0.61 0.48 0 0 0 0.15 0.09 0.11 0.1 0.13 0.13 0.17 0.13 0.11
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.1 0.07 0.09 0 0 0.05 0 0.06 0 0 0 0.05 0.16 0.14 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 2 0 0 1 0 0 1 0 0 0 3 0 0 4 2 7 2 5 37 8 14 20
Bud 1 4 3 2 1 3 6 6 9 12 22 12 0 0 0 2 0 0 0 2 2 4 14 17
Bud Neck 0 0 0 0 1 0 0 0 0 0 0 0 3 1 3 1 1 1 1 5 9 4 10 8
Bud Site 0 0 0 0 0 0 1 0 0 0 2 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 5 7 2 11 7 6 4 8 10 4 9 6 5 7 4 17 12 1 6 9 11 26 22
Cytoplasm 121 180 270 135 311 309 478 448 342 434 292 347 164 313 333 69 164 72 56 127 64 100 265 270
Endoplasmic Reticulum 14 19 63 45 41 18 7 14 14 12 6 10 7 8 5 6 12 2 79 66 276 114 141 76
Endosome 3 0 3 2 17 5 17 10 8 15 2 16 0 0 0 3 7 1 3 1 4 1 2 4
Golgi 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 4 1 0 1 0 3 4
Mitochondria 3 13 29 32 38 141 215 269 268 342 346 288 0 0 0 15 21 13 18 34 63 52 76 57
Nucleus 0 0 0 3 0 2 2 3 2 2 1 2 0 0 1 5 3 1 0 0 0 0 1 0
Nuclear Periphery 1 0 0 1 2 0 1 3 1 2 0 1 0 0 0 0 0 0 0 0 0 1 0 0
Nucleolus 0 0 0 0 0 0 0 1 1 0 3 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 1 0 1 0 1 0 0 2 0 3 0 0 0 1 0 0 0 0 1 0 0 0
SpindlePole 0 0 0 1 2 0 6 6 4 10 6 10 0 1 0 2 2 0 1 1 2 0 2 2
Vac/Vac Membrane 6 0 8 8 42 30 65 26 23 33 14 34 0 1 0 5 37 16 6 13 5 13 20 19
Unique Cell Count 147 211 366 214 419 429 691 684 557 728 569 605 174 323 338 98 238 117 176 270 487 317 589 515
Labelled Cell Count 150 222 386 231 467 516 806 790 681 874 698 732 183 330 349 117 266 129 176 270 487 317 589 515


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 3.9 4.6 4.9 4.9 4.0 3.7 3.7 3.5 3.3 3.4 3.5 5.7 5.5 5.7 5.3 6.5 7.9 6.1 5.9 6.0
Std Deviation (1e-4) 1.4 0.4 1.0 1.4 1.7 1.5 1.3 1.0 1.1 0.9 0.8 0.9 1.1 0.9 1.1 0.8 1.2 1.4 1.5 1.6 1.5
Intensity Change (Log2) – – – 0.09 0.09 -0.21 -0.32 -0.3 -0.41 -0.46 -0.46 -0.41 0.3 0.25 0.31 0.19 0.49 0.78 0.4 0.35 0.38


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 0
Bud Neck – – – – – – – – – – – – – 0 0
Bud Site – – – – – – – – – – – – – 0 0
Cell Periphery – – – – – – – – – – – – – 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 0
Endosome – – – – – – – – – – – – – 0 0
Golgi – – – – – – – – – – – – – 0 0
Mitochondria – – – – – – – – – – – – – 0 0
Nuclear Periphery – – – – – – – – – – – – – 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 0
Peroxisomes – – – – – – – – – – – – – 0 0
SpindlePole – – – – – – – – – – – – – 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 0
Cytoplasm – – – – – – – – – – – – – 0 0
Nucleus – – – – – – – – – – – – – 0 0
Vacuole – – – – – – – – – – – – – 6.1 5.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.4311 0.9158 0.5165 0.578 -0.0458 0.4834 0.3315 1.0381 0.8508 0.749 0.1192 0.5704 -0.4423 0.5948 0.0935 0.5056 -0.2111 0.6206
Actin 0.035 0.0107 0.0156 0.0019 0.022 0.0052 0.0166 0.0004 0.0223 0.0015 0.0105 0.0029 0.0148 0.0039 0.0043 0.0329 0.0057 0.0027
Bud 0.0018 0.0005 0.0007 0.0004 0.0008 0.0001 0.0005 0 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0003 0.0003 0.0001 0.0001
Bud Neck 0.0054 0.0004 0.0007 0.0013 0.0006 0.0025 0.0003 0.0003 0.0033 0.0003 0.0006 0.0009 0.0003 0.0003 0.0038 0.0033 0.0002 0.0009
Bud Periphery 0.0032 0.0008 0.0011 0.0005 0.0027 0.0002 0.0005 0 0.0005 0.0002 0.0002 0.0002 0.0002 0.0002 0.0009 0.0005 0.0002 0.0003
Bud Site 0.0056 0.0106 0.0033 0.0036 0.0009 0.0001 0.0026 0.001 0.002 0.0001 0.0001 0.0001 0.0005 0.002 0.002 0.0007 0.0002 0.0001
Cell Periphery 0.0014 0.0014 0.0013 0.0009 0.0003 0.0004 0.0062 0.0009 0.0049 0.0011 0.0011 0.0006 0.0019 0.0021 0.0026 0.0009 0.0003 0.001
Cytoplasm 0.2253 0.3713 0.3539 0.3301 0.3008 0.4168 0.29 0.2938 0.4165 0.2519 0.4429 0.3334 0.3588 0.386 0.3793 0.4485 0.3748 0.442
Cytoplasmic Foci 0.0294 0.0157 0.0154 0.0145 0.0206 0.0158 0.0225 0.0127 0.011 0.0036 0.0087 0.0148 0.01 0.008 0.0123 0.0104 0.008 0.0164
Eisosomes 0.0005 0.0002 0.0005 0.0003 0.0005 0.0001 0.0004 0.0001 0.0007 0.0001 0.0006 0.0002 0.0003 0.0006 0.0003 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.1896 0.1926 0.1542 0.1569 0.1701 0.19 0.2315 0.2848 0.253 0.4746 0.0528 0.1885 0.2068 0.2224 0.1794 0.2678 0.207 0.2151
Endosome 0.0439 0.0087 0.0133 0.0302 0.0209 0.0153 0.0393 0.0331 0.0102 0.0108 0.0075 0.0166 0.0171 0.0086 0.0124 0.0079 0.0113 0.0103
Golgi 0.0134 0.0013 0.0047 0.0025 0.013 0.0044 0.013 0.001 0.0026 0.0015 0.0007 0.001 0.002 0.001 0.0027 0.0062 0.005 0.0014
Lipid Particles 0.0254 0.0041 0.0083 0.0061 0.0178 0.0079 0.0165 0.0072 0.014 0.0056 0.0026 0.0065 0.0118 0.0071 0.0126 0.0044 0.0167 0.0053
Mitochondria 0.0053 0.0012 0.0062 0.0016 0.0121 0.0024 0.0473 0.0002 0.0168 0.0018 0.0004 0.0004 0.0011 0.0002 0.0061 0.0007 0.0021 0.0017
None 0.3653 0.3525 0.4052 0.4247 0.3736 0.3239 0.2905 0.334 0.2063 0.2317 0.4609 0.4211 0.3554 0.3459 0.3665 0.2031 0.3427 0.2864
Nuclear Periphery 0.0135 0.0055 0.0052 0.0078 0.0089 0.0069 0.0054 0.0154 0.0065 0.0085 0.0042 0.0062 0.0076 0.0053 0.0053 0.005 0.0112 0.0065
Nucleolus 0.0007 0.0012 0.0003 0.0005 0.0006 0.0002 0.0002 0.0002 0.0007 0 0.0001 0.0001 0.0002 0.0001 0.0004 0.0001 0.0001 0.0001
Nucleus 0.0031 0.0023 0.0024 0.0044 0.0021 0.0022 0.0022 0.0051 0.0023 0.0019 0.0017 0.0012 0.0017 0.0013 0.0015 0.0016 0.0022 0.0019
Peroxisomes 0.0124 0.0005 0.002 0.0005 0.0157 0.0006 0.0041 0.0001 0.0166 0 0.0002 0.0003 0.0007 0.0001 0.0018 0.0022 0.0082 0.0034
Punctate Nuclear 0.0102 0.002 0.0018 0.0024 0.0119 0.0024 0.0017 0.0035 0.0028 0.0003 0.0008 0.0023 0.0025 0.0006 0.0013 0.0012 0.001 0.0013
Vacuole 0.008 0.0149 0.0034 0.0079 0.0029 0.0022 0.0073 0.0059 0.0065 0.0038 0.0031 0.0023 0.0055 0.0039 0.0036 0.0021 0.0025 0.0028
Vacuole Periphery 0.0013 0.0017 0.0004 0.0009 0.0014 0.0004 0.0014 0.0004 0.0004 0.0005 0.0003 0.0003 0.0007 0.0002 0.0007 0.0002 0.0005 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.8702 9.7155 11.7625 11.0488 10.3829 9.1471 14.9935 12.3878 10.7468 15.4183
Translational Efficiency 0.6941 0.9011 0.6565 0.4658 0.6335 0.9584 0.5225 0.4464 0.5548 0.4912

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
150 628 2076 848 1694 370 184 689 1844 998 2260 1537

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 909.00 716.70 845.41 857.94 687.40 741.61 905.97 877.23 705.43 725.94 850.34 866.59
Standard Deviation 138.13 107.97 97.54 126.18 104.72 105.57 98.21 117.59 123.68 107.76 98.99 122.78
Intensity Change Log 2 — -0.342911 -0.104629 -0.083404 — 0.109511 0.398313 0.351805 — -0.130525 0.133670 0.120255

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.007273 0.001114 0.000280 0.002298 0.000286 0.000390 0.000770 0.004427 0.000854 0.000846 0.000320 0.003252
Bud Neck 0.003991 0.012530 0.007499 0.012023 0.009270 0.010026 0.016939 0.007044 0.008841 0.011602 0.008268 0.009791
Bud Site 0.033353 0.008376 0.012549 0.038286 0.002778 0.004883 0.015071 0.023069 0.005265 0.007081 0.012754 0.031464
Cell Periphery 0.001544 0.000625 0.000243 0.000845 0.000384 0.000352 0.000700 0.000827 0.000479 0.000524 0.000280 0.000837
Cytoplasm 0.284531* 0.343464* 0.402200* 0.379113* 0.403025* 0.374606* 0.318548* 0.363995* 0.393386* 0.355010* 0.395389* 0.372336*
Cytoplasmic Foci 0.078154 0.240517* 0.016714 0.051124 0.210417* 0.225486* 0.038861 0.083232 0.199658* 0.234944* 0.018517 0.065517
Eisosomes 0.000156 0.000845 0.000047 0.000343 0.000728 0.000427 0.000224 0.000292 0.000682 0.000690 0.000061 0.000320
Endoplasmic Reticulum 0.003445 0.097097 0.006806 0.036305 0.057655 0.043614 0.070568 0.035110 0.053245 0.077269 0.011998 0.035769
Endosome 0.020398 0.024426 0.002513 0.009788 0.012875 0.020375 0.008290 0.013304 0.013487 0.022924 0.002984 0.011364
Golgi 0.031665 0.014272 0.000086 0.003153 0.007935 0.004688 0.000583 0.001853 0.009865 0.010719 0.000127 0.002570
Lipid Particles 0.002881 0.069030 0.002030 0.010228 0.074040 0.067190 0.011044 0.017040 0.068252 0.068348 0.002764 0.013281
Mitochondria 0.004551 0.007360 0.000865 0.002378 0.005306 0.006767 0.000605 0.004291 0.005245 0.007140 0.000844 0.003236
Mitotic Spindle 0.004345 0.001869 0.004401 0.006756 0.000558 0.002279 0.005292 0.018182 0.000866 0.002021 0.004474 0.011878
None 0.000621 0.006346 0.003981 0.002901 0.003048 0.002778 0.005425 0.005137 0.002851 0.005023 0.004098 0.003903
Nuclear Periphery 0.000911 0.003372 0.004173 0.002162 0.004852 0.009732 0.004218 0.005698 0.004531 0.005730 0.004177 0.003747
Nuclear Periphery Foci 0.002656 0.007637 0.003109 0.012005 0.003702 0.002785 0.019907 0.022592 0.003617 0.005838 0.004477 0.016751
Nucleolus 0.000164 0.002003 0.000444 0.000634 0.001718 0.001194 0.000381 0.000409 0.001591 0.001703 0.000439 0.000533
Nucleus 0.116079 0.081854 0.481585* 0.207868* 0.150934* 0.134426* 0.270331* 0.129322 0.148099* 0.101345 0.464386* 0.172658*
Peroxisomes 0.001080 0.010178 0.000365 0.002688 0.005314 0.006169 0.001001 0.004642 0.004970 0.008692 0.000417 0.003564
Vacuole 0.394985* 0.064674 0.049923 0.217975* 0.044055 0.078262 0.210911* 0.258226* 0.072602 0.069712 0.063030 0.236019*
Vacuole Periphery 0.007220 0.002408 0.000185 0.001127 0.001120 0.003571 0.000332 0.001310 0.001616 0.002839 0.000197 0.001209

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.90 1.26 0.58 -1.70 -3.95 -0.50 -3.53 -2.12 -2.04 -1.70 -1.27 0.89 -2.86 -2.44 -3.28
Bud Neck -5.13 -3.90 -5.67 1.19 -1.84 -0.32 -1.39 2.95 1.60 2.28 -1.87 0.31 0.28 2.16 -0.08
Bud Site 2.16 1.71 -0.66 -5.73 -4.63 -1.29 -3.24 -5.98 -4.67 -0.99 -2.25 -4.43 -8.43 -7.29 -4.82
Cell Periphery 0.22 1.07 0.12 -0.54 -7.60 0.69 -3.31 -1.25 -1.40 0.04 -1.77 3.14 -2.35 -1.27 -4.02
Cytoplasm -5.01 -6.72 -4.93 0.31 2.88 2.09 6.05 6.23 2.87 -2.09 3.55 3.26 6.19 1.99 3.23
Cytoplasmic Foci -18.33 6.09 1.80 23.69 -10.02 -1.45 28.42 24.06 14.98 -6.08 -4.98 41.72 30.02 27.47 -15.65
Eisosomes -3.29 5.85 -4.28 2.61 -7.63 2.73 6.01 5.48 2.35 -0.70 -0.04 8.05 5.28 3.07 -8.84
Endoplasmic Reticulum -11.25 -4.91 -7.67 7.95 -6.41 2.05 0.60 7.08 2.85 2.34 -3.58 12.12 6.95 8.41 -6.31
Endosome -3.39 3.19 1.55 7.93 -4.83 -2.42 5.01 2.02 3.13 -2.03 -5.74 15.45 4.37 7.89 -7.29
Golgi -0.27 2.34 1.97 6.69 -1.67 3.69 12.25 9.87 4.49 -1.53 -1.95 12.13 7.41 7.84 -2.32
Lipid Particles -14.82 2.07 -7.16 13.55 -9.33 1.17 20.75 19.55 9.66 -1.77 0.05 24.71 21.21 16.49 -10.49
Mitochondria -4.03 1.10 0.29 4.62 -0.82 -0.98 6.80 1.83 2.25 -1.73 -2.14 6.43 2.99 4.82 -1.66
Mitotic Spindle 0.49 -1.58 -1.61 -2.15 -0.30 -1.22 -1.42 -4.16 -3.45 -1.65 -1.64 -4.16 -5.09 -4.09 -2.41
None -3.77 -4.94 -1.99 2.41 1.31 0.12 -0.33 -0.32 -0.34 0.11 -2.02 -1.12 -0.03 1.67 0.73
Nuclear Periphery -2.73 -7.79 -4.94 1.27 4.71 -2.00 1.34 -0.05 1.96 -1.29 -0.99 0.36 1.67 2.10 1.73
Nuclear Periphery Foci -4.97 -0.40 -7.32 -1.92 -7.02 1.88 -4.60 -5.72 -6.10 -0.49 -3.37 -0.87 -7.98 -5.77 -7.30
Nucleolus -2.99 -1.50 -1.35 2.44 -0.59 1.31 3.76 3.65 3.85 -0.70 -0.16 3.54 3.27 3.21 -0.49
Nucleus 2.42 -24.01 -5.86 -15.33 29.00 1.98 -6.84 2.51 0.24 7.58 8.70 -45.80 -4.44 -11.70 37.71
Peroxisomes -9.33 1.92 -1.65 7.37 -2.50 -0.62 8.92 2.37 2.08 -3.10 -4.22 10.69 4.06 7.02 -4.43
Vacuole 16.42 16.34 7.72 -22.83 -23.41 -5.61 -11.66 -26.51 -21.01 -2.91 2.24 -1.03 -28.60 -31.17 -29.12
Vacuole Periphery 0.24 1.25 1.00 5.29 -2.17 -2.67 7.71 0.82 2.79 -1.51 -3.82 5.66 1.79 4.95 -2.71
ORF
Human Ortholog
Description Putative protein of unknown function; predicted to contain a transmembrane domain; not an essential gene; YNL095C has a paralog, ECM3, that arose from the whole genome duplication
Localization
Cell Percentages ER (44%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

YNL095C

YNL095C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ynl095c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available