Standard name
Human Ortholog
Description GTP-binding protein; regulates nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes; RAS2 has a paralog, RAS1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.97 0.99 0.99 0.94 0.94 0.9 – 0.91 0.93 0.9 0.97 0.98 0.99 0.98 0.99 0.98 0.93 0.88 0.83 0.85 0.82 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.05 – 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 1 2 – 2 0 1 0 0 0 1 0 0 0 0 0 0 1 1
Bud 0 0 0 0 1 3 4 8 – 3 0 5 0 0 0 0 0 0 0 0 0 0 0 3
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 4 4 12 5 18 – 14 5 9 0 5 2 6 1 4 0 0 0 0 0 0
Cytoplasm 89 114 76 201 295 326 338 478 – 261 199 227 127 282 266 284 232 172 85 92 72 100 152 205
Endoplasmic Reticulum 0 1 1 2 1 4 6 11 – 5 5 2 2 2 1 10 9 5 0 1 3 3 4 2
Endosome 0 0 0 0 0 0 0 1 – 0 0 0 0 1 0 0 0 0 0 0 1 0 1 0
Golgi 0 0 0 0 0 2 0 9 – 4 0 2 0 0 0 1 0 0 0 0 0 0 0 0
Mitochondria 0 0 2 1 0 3 2 0 – 3 4 10 0 0 0 1 2 0 0 0 0 0 0 2
Nucleus 0 0 0 0 2 2 1 0 – 0 2 2 2 0 0 0 0 0 1 2 1 3 6 3
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 0 0 1 4 12 27 – 9 3 4 2 2 1 1 3 2 0 3 1 6 8 4
Unique Cell Count 90 115 78 203 298 345 360 530 286 215 251 131 287 268 289 234 175 92 105 87 119 186 230
Labelled Cell Count 90 115 79 208 304 357 369 554 301 218 262 133 292 270 304 247 183 92 105 87 119 186 230


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 40.9 45.5 29.5 30.5 27.8 28.0 26.7 31.6 – 30.9 28.9 28.0 31.7 38.7 41.3 30.0 29.9 30.8 43.7 41.8 42.9
Std Deviation (1e-4) 11.2 13.6 12.9 10.9 9.5 8.5 9.6 10.1 – 10.1 11.1 10.0 9.4 10.7 11.7 8.8 8.8 9.3 13.5 15.1 13.6
Intensity Change (Log2) – – – 0.05 -0.08 -0.07 -0.14 0.1 – 0.07 -0.03 -0.07 0.1 0.39 0.48 0.03 0.02 0.06 0.57 0.5 0.54


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 1.1 -1.1 -1.2 -2.1 – -1.8 -1.5 -2.0 -0.2 0.5 1.3 0.5 1.2 0.4
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 32.7859 40.1869 36.2268 35.1495 28.5061 37.4532 38.2441 36.4184 38.95 38.5511 36.051 39.2925 35.7505 36.9085 35.0537 32.851 30.0729 40.3966
Actin 0.0073 0.0025 0.0136 0.0001 0.0003 0.0001 0.0048 0.0008 0.0034 0 0.0009 0.0012 0.0019 0.0002 0.006 0.054 0.0323 0.0001
Bud 0.0005 0.0001 0.0009 0 0.0001 0.0001 0.0011 0 0.0004 0 0.0004 0.0001 0.0003 0 0.0001 0.0002 0.0006 0
Bud Neck 0.0008 0.0002 0.0005 0.0003 0.0004 0.0004 0.0004 0.0001 0.0002 0.0001 0.0003 0.0006 0.0001 0.0001 0.0004 0.0004 0.0005 0.0004
Bud Periphery 0.0006 0.0001 0.0003 0 0 0 0.0006 0 0.0005 0 0.0006 0.0001 0.0001 0 0.0001 0.0002 0.0009 0
Bud Site 0.0013 0.0002 0.0035 0 0.0001 0 0.0012 0.0001 0.0003 0 0.0003 0.0001 0.0002 0 0.0012 0.0005 0.0031 0
Cell Periphery 0.0003 0 0.0001 0 0 0 0.0002 0 0.0002 0 0.0003 0.0001 0 0 0.0001 0 0.0002 0
Cytoplasm 0.8836 0.986 0.9516 0.9885 0.9083 0.985 0.9313 0.9788 0.9624 0.9901 0.9008 0.9584 0.9405 0.9935 0.9554 0.9322 0.8392 0.9842
Cytoplasmic Foci 0.0197 0.0013 0.0094 0.0003 0.0153 0.0006 0.0128 0.0029 0.003 0.0002 0.0033 0.0034 0.0076 0.0004 0.0077 0.0005 0.0042 0.0002
Eisosomes 0.0001 0 0.0001 0 0 0 0.0001 0 0 0 0.0002 0 0 0 0.0001 0.0003 0.0004 0
Endoplasmic Reticulum 0.0092 0.0004 0.0004 0.0001 0.0007 0.0002 0.0021 0.0003 0.0007 0.0002 0.0016 0.0034 0.0011 0.0003 0.0009 0.0003 0.0088 0.0001
Endosome 0.0159 0.0005 0.0017 0 0.033 0.0002 0.0064 0.0005 0.0017 0.0001 0.005 0.0031 0.0047 0.0001 0.0025 0.0006 0.0148 0
Golgi 0.0042 0.0004 0.0018 0 0.0244 0 0.0014 0.0007 0.0006 0 0.0009 0.001 0.0011 0 0.0013 0.0014 0.0077 0
Lipid Particles 0.0076 0.0011 0.0005 0 0.0024 0 0.0055 0.0013 0.0017 0 0.0027 0.0013 0.0058 0 0.0041 0.0003 0.0086 0
Mitochondria 0.0029 0.0007 0.0017 0 0.0006 0.0001 0.0023 0.0001 0.0066 0 0.0584 0.0014 0.0014 0 0.0032 0.0005 0.0282 0
None 0.0101 0.0003 0.0019 0.0001 0.0003 0.0002 0.0028 0.0001 0.0007 0.0001 0.0035 0.0006 0.002 0.0002 0.0023 0.0002 0.0015 0.0001
Nuclear Periphery 0.0078 0.0007 0.001 0.001 0.0018 0.0013 0.0045 0.0026 0.0019 0.0016 0.0027 0.0068 0.0055 0.001 0.0024 0.0012 0.0213 0.0013
Nucleolus 0.0003 0.0002 0.0006 0 0 0 0.0003 0 0.0005 0 0.0004 0.0014 0.0001 0 0.001 0 0.0005 0
Nucleus 0.0194 0.0048 0.0061 0.0093 0.007 0.0114 0.0136 0.0081 0.0077 0.0071 0.0071 0.0115 0.0187 0.0038 0.0072 0.0064 0.018 0.0131
Peroxisomes 0.0015 0.0001 0.001 0 0.0011 0 0.0015 0.0028 0.0008 0 0.0012 0.0004 0.0058 0 0.0006 0.0002 0.002 0
Punctate Nuclear 0.0031 0.0002 0.0015 0.0002 0.0003 0.0002 0.0024 0.0003 0.0004 0.0001 0.0002 0.0017 0.0013 0.0001 0.0017 0.0002 0.0026 0.0001
Vacuole 0.0028 0.0002 0.0015 0.0001 0.0035 0.0002 0.0039 0.0003 0.0039 0.0002 0.0054 0.002 0.0009 0.0001 0.0008 0.0002 0.0017 0.0001
Vacuole Periphery 0.0011 0.0001 0.0003 0 0.0005 0.0001 0.0012 0.0001 0.0024 0.0001 0.0039 0.0016 0.0009 0 0.0007 0.0001 0.0029 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 77.4319 76.3449 96.6847 120.9727 98.9175 70.5606 79.7653 109.0618 135.6151 103.401
Translational Efficiency 1.8047 1.5128 1.3852 1.437 1.3844 2.0584 1.6874 1.4355 1.4563 1.4599

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
858 1504 2666 1295 2005 962 1312 1297 2863 2466 3978 2592

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1950.98 2272.04 2581.21 2467.68 1887.48 2145.29 2290.45 2334.27 1906.51 2222.59 2485.31 2400.92
Standard Deviation 479.03 458.26 461.50 532.00 404.33 498.36 469.10 498.33 429.08 478.32 483.74 519.72
Intensity Change Log 2 — 0.219789 0.403849 0.338956 — 0.184711 0.279170 0.306510 — 0.202647 0.343886 0.323093

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000544 0.000151 0.000220 0.000923 0.000065 0.000531 0.000164 0.000650 0.000208 0.000299 0.000202 0.000786
Bud Neck 0.001405 0.000609 0.000410 0.004912 0.000188 0.001544 0.001712 0.002812 0.000553 0.000974 0.000839 0.003861
Bud Site 0.003686 0.001691 0.000867 0.017040 0.000851 0.006819 0.002523 0.015441 0.001701 0.003691 0.001413 0.016240
Cell Periphery 0.000447 0.000297 0.000089 0.000610 0.000094 0.000584 0.000160 0.000259 0.000200 0.000409 0.000112 0.000434
Cytoplasm 0.795571* 0.864948* 0.763239* 0.716004* 0.897564* 0.832105* 0.776514* 0.750073* 0.866998* 0.852136* 0.767617* 0.733052*
Cytoplasmic Foci 0.009405 0.004226 0.001836 0.007235 0.002409 0.016417 0.005710 0.011961 0.004506 0.008981 0.003114 0.009600
Eisosomes 0.000003 0.000001 0.000001 0.000008 0.000000 0.000003 0.000002 0.000006 0.000001 0.000002 0.000001 0.000007
Endoplasmic Reticulum 0.000651 0.000141 0.000016 0.000333 0.000128 0.000471 0.000319 0.000222 0.000284 0.000270 0.000116 0.000278
Endosome 0.000984 0.000289 0.000021 0.003901 0.000088 0.001456 0.002017 0.005696 0.000356 0.000744 0.000679 0.004799
Golgi 0.004164 0.001153 0.000087 0.001122 0.000500 0.004127 0.002220 0.005450 0.001598 0.002313 0.000791 0.003288
Lipid Particles 0.000872 0.000474 0.000150 0.002033 0.000162 0.001714 0.000629 0.001121 0.000375 0.000958 0.000308 0.001577
Mitochondria 0.001568 0.000124 0.000105 0.000844 0.000126 0.000865 0.000173 0.001426 0.000558 0.000413 0.000127 0.001135
Mitotic Spindle 0.006407 0.001724 0.000331 0.018563 0.000710 0.005598 0.012542 0.022803 0.002417 0.003235 0.004359 0.020684
None 0.004528 0.005496 0.013622 0.012368 0.004835 0.004075 0.008923 0.008698 0.004743 0.004942 0.012072 0.010532
Nuclear Periphery 0.000450 0.000095 0.000064 0.000482 0.000053 0.000198 0.000313 0.000466 0.000172 0.000135 0.000146 0.000474
Nuclear Periphery Foci 0.004942 0.001914 0.000291 0.004466 0.001040 0.004639 0.001944 0.004615 0.002210 0.002977 0.000836 0.004540
Nucleolus 0.000827 0.000088 0.000088 0.000294 0.000060 0.000483 0.000139 0.000597 0.000290 0.000242 0.000105 0.000446
Nucleus 0.135154* 0.109327 0.214750* 0.186634* 0.086057 0.097514 0.160965* 0.146102* 0.100770 0.104719 0.197011* 0.166352*
Peroxisomes 0.000054 0.000012 0.000031 0.000224 0.000010 0.000085 0.000029 0.001362 0.000023 0.000040 0.000030 0.000793
Vacuole 0.017956 0.006809 0.003750 0.021444 0.003851 0.015683 0.022490 0.017884 0.008078 0.010271 0.009931 0.019663
Vacuole Periphery 0.010386 0.000433 0.000031 0.000561 0.001207 0.005091 0.000510 0.002357 0.003958 0.002250 0.000189 0.001460

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 5.07 2.15 -0.35 -4.61 -2.29 -7.36 -6.27 -4.91 2.65 -2.09 -3.21 -0.23 -4.47 -2.27 -2.37
Bud Neck 3.82 4.10 -5.28 -7.44 -7.57 -9.93 -8.07 -9.66 -1.21 0.55 -6.49 -6.53 -11.00 -7.28 -6.61
Bud Site 3.55 5.45 -3.36 -5.36 -6.19 -6.52 -8.30 -7.23 -0.75 -4.46 -4.57 -0.23 -8.17 -4.93 -8.16
Cell Periphery 2.50 6.65 -2.66 -4.83 -8.51 -11.31 -7.67 -7.79 6.09 -1.30 -7.28 -0.37 -8.34 -0.60 -8.50
Cytoplasm -8.42 6.50 14.19 28.33 11.75 13.43 29.16 26.74 8.86 -3.07 6.42 32.73 33.27 26.64 6.35
Cytoplasmic Foci 6.02 7.56 -1.62 -9.60 -11.62 -13.65 -11.55 -14.17 3.21 -4.84 -9.22 -1.93 -13.78 -3.79 -12.95
Eisosomes 5.32 4.72 -5.17 -9.65 -9.22 -8.92 -13.07 -10.20 -2.23 -2.13 -3.93 -4.65 -11.84 -9.60 -9.72
Endoplasmic Reticulum 4.33 5.63 4.37 0.49 -3.36 -4.28 -1.14 0.88 5.08 1.89 -0.04 4.37 3.61 4.55 -1.20
Endosome 3.99 6.40 -0.19 -1.59 -2.29 -5.26 -2.39 -3.23 -0.15 -1.07 -3.58 0.26 -2.93 -1.56 -2.88
Golgi 3.16 4.55 4.08 3.94 -3.94 -6.03 -1.17 -1.56 3.02 -0.64 -2.21 3.88 1.37 3.37 -1.70
Lipid Particles 2.28 4.36 -7.76 -9.51 -10.68 -10.70 -8.96 -8.70 0.76 -1.89 -8.81 -5.23 -12.77 -7.13 -10.38
Mitochondria 1.29 1.30 1.11 -1.51 -1.57 -1.68 -0.73 -1.63 0.87 -1.47 0.42 1.26 0.51 0.12 -2.17
Mitotic Spindle 1.90 2.83 -0.28 -2.76 -4.03 -4.17 -3.96 -3.85 -0.20 0.16 -2.02 -0.89 -3.69 -2.23 -3.15
None -2.03 -12.21 -2.99 -1.84 7.12 2.19 -3.60 -3.02 -4.69 1.09 -0.53 -14.10 -3.99 -3.68 8.28
Nuclear Periphery 1.63 1.58 0.11 -6.63 -6.50 -8.69 -8.03 -4.91 -1.00 1.80 -0.03 -0.97 -2.82 -5.47 -3.61
Nuclear Periphery Foci 2.66 4.48 1.35 -2.16 -5.82 -10.22 -3.46 -3.50 2.99 -1.53 -3.46 3.33 -2.30 0.36 -5.48
Nucleolus 1.62 1.62 1.36 -3.62 -3.23 -1.22 -4.40 -1.88 0.32 -0.95 0.21 1.11 0.00 -0.26 -1.83
Nucleus 3.78 -16.00 -7.52 -13.82 8.60 -4.38 -20.29 -11.35 -5.34 9.17 -2.96 -33.84 -16.31 -13.36 14.91
Peroxisomes 4.21 1.44 -1.15 -2.96 -1.95 -3.50 -6.46 -2.04 -1.59 -1.90 -2.08 -0.89 -2.28 -2.06 -2.17
Vacuole 5.72 6.32 -15.61 -20.24 -20.68 -9.63 -16.95 -20.38 -13.16 -2.31 -4.08 -11.52 -26.83 -24.63 -19.70
Vacuole Periphery 3.77 3.95 3.88 1.60 -2.45 -2.10 1.77 0.99 2.63 -1.25 1.73 4.42 3.99 2.74 -2.06
Standard name
Human Ortholog
Description GTP-binding protein; regulates nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes; RAS2 has a paralog, RAS1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (95%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Ras2

Ras2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ras2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available