Standard name | |
---|---|
Human Ortholog | |
Description | Cytochrome b5; involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 13 | 12 | 10 | 12 | 16 | 10 | – | 21 | 9 | 10 | 5 | 17 | 14 | 14 | 5 | 11 | 8 | 9 | 8 | 5 | 5 | 3 | 8 | 3 |
Bud | 1 | 1 | 13 | 4 | 2 | 7 | – | 9 | 5 | 8 | 12 | 13 | 2 | 2 | 2 | 1 | 0 | 1 | 5 | 2 | 2 | 1 | 2 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 4 | 1 | 0 | 2 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 5 | 0 | 8 | 11 | 24 | – | 18 | 14 | 12 | 19 | 23 | 26 | 44 | 56 | 25 | 12 | 17 | 0 | 0 | 0 | 0 | 1 | 0 |
Cytoplasm | 77 | 77 | 76 | 46 | 69 | 113 | – | 189 | 153 | 140 | 164 | 207 | 52 | 84 | 123 | 165 | 113 | 123 | 29 | 8 | 14 | 10 | 12 | 18 |
Endoplasmic Reticulum | 264 | 251 | 174 | 134 | 159 | 201 | – | 238 | 157 | 128 | 156 | 220 | 316 | 303 | 362 | 5 | 1 | 4 | 350 | 348 | 347 | 210 | 343 | 342 |
Endosome | 15 | 7 | 23 | 11 | 38 | 22 | – | 7 | 12 | 5 | 2 | 13 | 41 | 41 | 37 | 16 | 11 | 15 | 11 | 6 | 12 | 2 | 5 | 9 |
Golgi | 0 | 3 | 0 | 1 | 1 | 1 | – | 1 | 0 | 0 | 0 | 0 | 4 | 1 | 2 | 2 | 2 | 3 | 1 | 4 | 1 | 0 | 2 | 7 |
Mitochondria | 1 | 1 | 43 | 26 | 8 | 29 | – | 29 | 32 | 21 | 44 | 73 | 0 | 0 | 0 | 4 | 3 | 4 | 3 | 2 | 10 | 3 | 5 | 6 |
Nucleus | 1 | 0 | 1 | 0 | 0 | 0 | – | 6 | 1 | 1 | 1 | 0 | 1 | 1 | 2 | 1 | 4 | 6 | 0 | 0 | 0 | 1 | 1 | 2 |
Nuclear Periphery | 0 | 3 | 4 | 5 | 4 | 2 | – | 11 | 8 | 4 | 7 | 10 | 5 | 6 | 4 | 1 | 0 | 1 | 9 | 7 | 8 | 5 | 2 | 2 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 4 | 1 | 2 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 6 | 1 | 0 | 2 | 2 | 3 | 2 | 0 | 0 | 0 |
Vac/Vac Membrane | 64 | 57 | 44 | 1 | 67 | 39 | – | 55 | 33 | 42 | 31 | 47 | 5 | 5 | 15 | 59 | 22 | 26 | 13 | 5 | 12 | 16 | 11 | 25 |
Unique Cell Count | 387 | 356 | 335 | 196 | 330 | 372 | 506 | 360 | 323 | 374 | 530 | 387 | 417 | 521 | 259 | 168 | 192 | 444 | 400 | 425 | 259 | 401 | 432 | |
Labelled Cell Count | 437 | 417 | 392 | 249 | 378 | 448 | 586 | 425 | 371 | 442 | 623 | 468 | 503 | 614 | 292 | 176 | 211 | 444 | 400 | 425 | 259 | 401 | 432 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.3 | 11.1 | 6.8 | 9.2 | 10.0 | 9.1 | – | 9.3 | 9.0 | 9.2 | 9.1 | 8.9 | 11.1 | 10.7 | 10.2 | 9.4 | 9.3 | 8.8 | 11.6 | 12.4 | 12.1 |
Std Deviation (1e-4) | 1.5 | 1.4 | 1.3 | 1.8 | 1.6 | 1.5 | – | 1.7 | 1.8 | 1.7 | 1.8 | 1.9 | 1.8 | 1.6 | 1.5 | 1.7 | 1.7 | 1.6 | 2.1 | 2.2 | 2.4 |
Intensity Change (Log2) | – | – | – | 0.44 | 0.56 | 0.42 | – | 0.45 | 0.42 | 0.44 | 0.42 | 0.39 | 0.72 | 0.66 | 0.59 | 0.48 | 0.46 | 0.37 | 0.78 | 0.87 | 0.83 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.8 | 1.2 | -0.2 | – | 0.9 | -0.4 | 0.1 | -1.5 | 0.2 | 0.5 | 0.3 | -2.2 | 0.8 | 1.0 | 1.0 |
Bud | -1.2 | -2.8 | -1.6 | – | -1.9 | -2.1 | -1.0 | -0.5 | -1.2 | -3.2 | -3.3 | -3.8 | -2.8 | 0 | -2.3 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 3.4 | 4.7 | – | 3.5 | 3.6 | 3.6 | 4.2 | 3.9 | 4.8 | 6.1 | 6.2 | 5.8 | 0 | 5.5 |
Cytoplasm | 0.2 | -0.6 | 2.3 | – | 4.5 | 5.6 | 5.6 | 5.9 | 5.0 | -3.2 | -0.8 | 0.3 | 10.1 | 9.7 | 9.4 |
Endoplasmic Reticulum | 3.7 | -1.0 | 0.6 | – | -1.4 | -2.2 | -3.2 | -2.7 | -3.0 | 8.5 | 5.9 | 5.2 | -13.2 | -11.4 | -11.6 |
Endosome | -0.6 | 2.1 | -0.5 | – | -4.2 | -2.1 | -3.4 | -4.6 | -3.2 | 1.8 | 1.4 | 0.1 | -0.3 | -0.1 | 0.4 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.1 | -5.0 | -2.2 | – | -3.6 | -1.7 | -2.7 | -0.4 | 0.4 | -7.3 | -7.5 | -8.4 | -5.1 | -4.1 | -4.2 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 1.1 | 1.0 | 0 | 0.7 | 0.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -5.0 | 2.5 | -1.1 | – | -1.0 | -1.7 | 0 | -2.1 | -2.0 | -6.3 | -6.6 | -5.8 | 3.1 | 0 | 0.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 9.5258 | 10.0755 | 9.4197 | 8.865 | 8.4197 | 9.6339 | 8.8611 | 10.1018 | 9.558 | 8.6287 | 7.6655 | 9.6387 | 10.8255 | 12.7982 | 12.0196 | 12.1624 | 10.7103 | 12.1355 |
Actin | 0.0017 | 0.0032 | 0.0121 | 0.0004 | 0.0364 | 0.0162 | 0.0236 | 0.0006 | 0.0102 | 0.0112 | 0.0164 | 0.002 | 0.0058 | 0.0004 | 0.0259 | 0.0387 | 0.0473 | 0.0003 |
Bud | 0 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 |
Bud Neck | 0.0003 | 0.0002 | 0.0006 | 0.0001 | 0.0016 | 0.0006 | 0.0007 | 0.0008 | 0.0001 | 0.0009 | 0.0004 | 0.0004 | 0.0004 | 0.0003 | 0.001 | 0.0006 | 0.0004 | 0.0004 |
Bud Periphery | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 |
Bud Site | 0.0002 | 0.0006 | 0.0003 | 0 | 0.0007 | 0.0002 | 0.0015 | 0.0011 | 0.0001 | 0.0002 | 0.0014 | 0.0001 | 0.0002 | 0.0002 | 0.0006 | 0.0001 | 0.0001 | 0 |
Cell Periphery | 0.0011 | 0.001 | 0.0005 | 0.0002 | 0.0006 | 0.0003 | 0.0009 | 0.0018 | 0.0007 | 0.0007 | 0.0004 | 0.0003 | 0.0006 | 0.0012 | 0.0006 | 0.0005 | 0.0005 | 0.0009 |
Cytoplasm | 0.0194 | 0.0392 | 0.017 | 0.0035 | 0.0111 | 0.02 | 0.0173 | 0.0412 | 0.0171 | 0.0533 | 0.0202 | 0.0167 | 0.0118 | 0.0841 | 0.0627 | 0.0356 | 0.0073 | 0.0226 |
Cytoplasmic Foci | 0.007 | 0.0111 | 0.0087 | 0.0012 | 0.0084 | 0.0062 | 0.0123 | 0.0101 | 0.0065 | 0.0076 | 0.0489 | 0.0045 | 0.006 | 0.0051 | 0.0175 | 0.002 | 0.0025 | 0.0036 |
Eisosomes | 0 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0003 | 0 |
Endoplasmic Reticulum | 0.7735 | 0.7635 | 0.8268 | 0.9459 | 0.7126 | 0.8209 | 0.7189 | 0.7569 | 0.8328 | 0.7365 | 0.6386 | 0.8463 | 0.785 | 0.7651 | 0.7568 | 0.855 | 0.7453 | 0.82 |
Endosome | 0.0155 | 0.0223 | 0.0206 | 0.0104 | 0.0197 | 0.0148 | 0.0462 | 0.0303 | 0.0262 | 0.0222 | 0.0777 | 0.0204 | 0.0335 | 0.0086 | 0.0148 | 0.0064 | 0.0142 | 0.0104 |
Golgi | 0.0042 | 0.0022 | 0.003 | 0.001 | 0.0055 | 0.0036 | 0.0151 | 0.0061 | 0.0046 | 0.0284 | 0.028 | 0.0046 | 0.0035 | 0.0015 | 0.0048 | 0.0037 | 0.0083 | 0.0017 |
Lipid Particles | 0.0812 | 0.0877 | 0.0426 | 0.0127 | 0.0633 | 0.0389 | 0.0825 | 0.1021 | 0.0716 | 0.0865 | 0.0778 | 0.0535 | 0.027 | 0.1025 | 0.0826 | 0.0412 | 0.0429 | 0.098 |
Mitochondria | 0.003 | 0.0003 | 0.0008 | 0.0008 | 0.0006 | 0.0004 | 0.0017 | 0.0012 | 0.0008 | 0.0189 | 0.0012 | 0.0017 | 0.0012 | 0.0001 | 0.0009 | 0.0005 | 0.0012 | 0.0002 |
None | 0.0003 | 0.0005 | 0.0016 | 0.0002 | 0.0032 | 0.0003 | 0.0046 | 0.0003 | 0.0003 | 0.0002 | 0.0034 | 0.0002 | 0.0022 | 0.0003 | 0.002 | 0.0002 | 0.002 | 0.0001 |
Nuclear Periphery | 0.0731 | 0.0525 | 0.0415 | 0.0168 | 0.0795 | 0.0635 | 0.0578 | 0.0303 | 0.0195 | 0.0175 | 0.066 | 0.0404 | 0.0992 | 0.0212 | 0.0148 | 0.0103 | 0.1097 | 0.0339 |
Nucleolus | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0012 | 0.0001 | 0.0002 | 0.0004 | 0 | 0 | 0.0001 | 0 | 0.0004 | 0.0001 | 0.0002 | 0 | 0.0003 | 0 |
Nucleus | 0.0028 | 0.0037 | 0.0041 | 0.0008 | 0.0051 | 0.004 | 0.0024 | 0.0034 | 0.0011 | 0.0103 | 0.004 | 0.0015 | 0.0089 | 0.0029 | 0.0017 | 0.0006 | 0.0023 | 0.0022 |
Peroxisomes | 0.0066 | 0.0002 | 0.0004 | 0 | 0.0023 | 0.0022 | 0.0025 | 0.0002 | 0.0003 | 0.0007 | 0.0016 | 0.0006 | 0.0005 | 0.0001 | 0.0021 | 0.0011 | 0.0021 | 0 |
Punctate Nuclear | 0.0011 | 0.0024 | 0.0133 | 0.0006 | 0.0417 | 0.0026 | 0.0026 | 0.0023 | 0.0005 | 0.0003 | 0.008 | 0.0013 | 0.0051 | 0.0009 | 0.007 | 0.0005 | 0.0072 | 0.0011 |
Vacuole | 0.0069 | 0.0081 | 0.004 | 0.0038 | 0.0043 | 0.0038 | 0.0066 | 0.0099 | 0.0062 | 0.0034 | 0.0038 | 0.0043 | 0.0066 | 0.0052 | 0.0031 | 0.0019 | 0.0028 | 0.0035 |
Vacuole Periphery | 0.0018 | 0.001 | 0.0015 | 0.0014 | 0.0013 | 0.0012 | 0.0019 | 0.0007 | 0.0011 | 0.0006 | 0.0016 | 0.0009 | 0.0018 | 0.0004 | 0.0005 | 0.0003 | 0.0027 | 0.0008 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 75.992 | 100.5397 | 62.5067 | 74.007 | 88.6743 | 54.684 | 48.0804 | 75.1009 | 62.9169 | 74.1765 |
Translational Efficiency | 2.122 | 1.8138 | 2.752 | 1.8692 | 2.1732 | 2.5051 | 3.2758 | 2.0409 | 2.3779 | 2.2498 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
438 | 1683 | 266 | 1212 | 1212 | 1526 | 193 | 1586 | 1650 | 3209 | 459 | 2798 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1087.60 | 1134.63 | 1412.30 | 1414.05 | 1190.66 | 1125.61 | 1492.92 | 1367.41 | 1163.30 | 1130.34 | 1446.20 | 1387.61 |
Standard Deviation | 166.13 | 175.74 | 214.03 | 242.39 | 193.78 | 165.28 | 187.49 | 224.45 | 192.30 | 170.90 | 207.15 | 233.54 |
Intensity Change Log 2 | — | 0.061074 | 0.376899 | 0.378685 | — | -0.081054 | 0.326375 | 0.199684 | — | -0.011456 | 0.350715 | 0.287910 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001341 | 0.000498 | 0.000376 | 0.007470 | 0.001335 | 0.000414 | 0.001400 | 0.007696 | 0.001337 | 0.000458 | 0.000807 | 0.007598 |
Bud Neck | 0.000537 | 0.000383 | 0.003042 | 0.007689 | 0.000193 | 0.000247 | 0.001931 | 0.008075 | 0.000284 | 0.000318 | 0.002575 | 0.007908 |
Bud Site | 0.002653 | 0.001641 | 0.001709 | 0.022419 | 0.001368 | 0.001134 | 0.002907 | 0.023137 | 0.001709 | 0.001400 | 0.002213 | 0.022826 |
Cell Periphery | 0.001041 | 0.000400 | 0.000530 | 0.002812 | 0.000742 | 0.000476 | 0.000768 | 0.001248 | 0.000821 | 0.000436 | 0.000630 | 0.001925 |
Cytoplasm | 0.010558 | 0.024490 | 0.033133 | 0.076494 | 0.009353 | 0.023504 | 0.012560 | 0.107134* | 0.009673 | 0.024021 | 0.024483 | 0.093862 |
Cytoplasmic Foci | 0.006829 | 0.010531 | 0.005240 | 0.013199 | 0.004016 | 0.007086 | 0.000743 | 0.034541 | 0.004763 | 0.008893 | 0.003349 | 0.025296 |
Eisosomes | 0.000118 | 0.000042 | 0.000027 | 0.000237 | 0.000037 | 0.000076 | 0.000021 | 0.000264 | 0.000059 | 0.000058 | 0.000025 | 0.000252 |
Endoplasmic Reticulum | 0.908614* | 0.771208* | 0.614108* | 0.601660* | 0.948877* | 0.808154* | 0.862501* | 0.406263* | 0.938189* | 0.788777* | 0.718552* | 0.490902* |
Endosome | 0.014218 | 0.035504 | 0.019495 | 0.027685 | 0.007925 | 0.025971 | 0.021221 | 0.038734 | 0.009595 | 0.030970 | 0.020221 | 0.033948 |
Golgi | 0.005725 | 0.014216 | 0.000407 | 0.006699 | 0.003201 | 0.010325 | 0.001256 | 0.010446 | 0.003871 | 0.012366 | 0.000764 | 0.008823 |
Lipid Particles | 0.012374 | 0.067033 | 0.016619 | 0.076119 | 0.005353 | 0.062893 | 0.019828 | 0.096736 | 0.007217 | 0.065064 | 0.017968 | 0.087806 |
Mitochondria | 0.000371 | 0.001414 | 0.000094 | 0.001002 | 0.000488 | 0.001988 | 0.000049 | 0.001488 | 0.000457 | 0.001687 | 0.000075 | 0.001277 |
Mitotic Spindle | 0.001400 | 0.001363 | 0.000709 | 0.017184 | 0.000556 | 0.000629 | 0.000184 | 0.043459 | 0.000780 | 0.001014 | 0.000488 | 0.032077 |
None | 0.000420 | 0.004806 | 0.000080 | 0.000948 | 0.001143 | 0.005069 | 0.000043 | 0.000659 | 0.000951 | 0.004931 | 0.000064 | 0.000784 |
Nuclear Periphery | 0.001582 | 0.003268 | 0.062359 | 0.003286 | 0.001231 | 0.002470 | 0.005067 | 0.010562 | 0.001324 | 0.002888 | 0.038269 | 0.007410 |
Nuclear Periphery Foci | 0.020266 | 0.054405 | 0.170783 | 0.070955 | 0.010490 | 0.045252 | 0.051946 | 0.118989 | 0.013085 | 0.050052 | 0.120815 | 0.098182 |
Nucleolus | 0.000102 | 0.000064 | 0.000062 | 0.000080 | 0.000093 | 0.000036 | 0.000005 | 0.000126 | 0.000096 | 0.000051 | 0.000038 | 0.000106 |
Nucleus | 0.000641 | 0.000631 | 0.006055 | 0.005714 | 0.000624 | 0.000630 | 0.000484 | 0.011224 | 0.000629 | 0.000631 | 0.003713 | 0.008837 |
Peroxisomes | 0.000461 | 0.000295 | 0.000109 | 0.000477 | 0.000222 | 0.000731 | 0.000014 | 0.001169 | 0.000285 | 0.000502 | 0.000069 | 0.000869 |
Vacuole | 0.005452 | 0.005990 | 0.064630 | 0.056305 | 0.002094 | 0.002055 | 0.016743 | 0.075739 | 0.002985 | 0.004119 | 0.044494 | 0.067321 |
Vacuole Periphery | 0.005298 | 0.001818 | 0.000431 | 0.001567 | 0.000660 | 0.000860 | 0.000332 | 0.002313 | 0.001891 | 0.001363 | 0.000390 | 0.001989 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.17 | 1.19 | -4.96 | -6.25 | -6.25 | 1.29 | -0.67 | -5.74 | -8.17 | -2.29 | 1.59 | -0.10 | -7.37 | -10.11 | -5.93 |
Bud Neck | 0.66 | -3.75 | -11.13 | -11.69 | -5.90 | -0.99 | -3.49 | -17.07 | -16.99 | -9.19 | -0.44 | -5.32 | -20.01 | -20.05 | -10.09 |
Bud Site | 0.97 | 0.37 | -9.75 | -10.82 | -10.50 | 0.54 | -1.35 | -12.41 | -12.52 | -6.85 | 0.73 | -1.42 | -16.13 | -16.51 | -12.66 |
Cell Periphery | 2.01 | 1.34 | -4.37 | -9.06 | -7.49 | 2.87 | -0.66 | -2.63 | -4.35 | -1.96 | 3.62 | 0.98 | -6.10 | -9.77 | -7.01 |
Cytoplasm | -6.51 | -4.20 | -15.50 | -12.56 | -6.54 | -7.72 | -0.93 | -22.07 | -18.99 | -16.96 | -10.23 | -4.26 | -26.89 | -22.62 | -15.29 |
Cytoplasmic Foci | -1.51 | 0.20 | -3.17 | -2.62 | -5.25 | -2.71 | 4.23 | -15.05 | -13.22 | -17.47 | -3.92 | 0.76 | -14.70 | -12.33 | -16.19 |
Eisosomes | 1.47 | 1.65 | -1.89 | -4.96 | -5.16 | -1.77 | 0.88 | -2.17 | -1.75 | -2.33 | 0.03 | 1.69 | -3.14 | -3.25 | -3.79 |
Endoplasmic Reticulum | 11.31 | 14.07 | 23.91 | 16.30 | 1.73 | 17.45 | 5.61 | 58.40* | 38.55* | 26.44* | 23.38 | 15.35 | 59.37 | 38.63* | 15.91 |
Endosome | -7.65 | -2.13 | -4.90 | 3.48 | -3.33 | -11.23 | -4.37 | -19.30 | -5.85 | -4.90 | -16.03 | -6.28 | -18.25 | -1.66 | -6.67 |
Golgi | -4.45 | 3.83 | -0.40 | 5.07 | -7.18 | -5.62 | 2.78 | -5.15 | 0.74 | -7.67 | -8.13 | 5.14 | -4.63 | 4.01 | -10.61 |
Lipid Particles | -11.60 | -1.48 | -12.93 | -2.16 | -11.05 | -13.90 | -2.40 | -21.41 | -6.28 | -10.27 | -19.06 | -3.57 | -25.28 | -6.15 | -15.94 |
Mitochondria | -3.09 | 3.00 | -2.73 | 1.04 | -4.11 | -2.97 | 3.80 | -2.79 | 1.01 | -4.36 | -4.06 | 4.27 | -3.58 | 1.30 | -5.75 |
Mitotic Spindle | 0.02 | 0.31 | -6.10 | -6.33 | -6.49 | -0.19 | 1.64 | -12.45 | -12.40 | -12.55 | -0.54 | 0.19 | -13.90 | -13.78 | -13.96 |
None | -5.04 | 1.82 | -1.88 | 4.37 | -3.90 | -3.53 | 2.63 | 1.16 | 4.26 | -3.73 | -5.43 | 2.84 | 0.54 | 6.16 | -5.34 |
Nuclear Periphery | -2.94 | -9.14 | -3.06 | -0.28 | 8.85 | -4.37 | -4.54 | -8.04 | -6.82 | -3.62 | -5.50 | -9.06 | -8.80 | -6.23 | 7.50 |
Nuclear Periphery Foci | -7.24 | -12.38 | -10.09 | -4.13 | 7.97 | -11.02 | -5.91 | -22.29 | -13.31 | -7.77 | -15.02 | -13.60 | -25.06 | -13.07 | 2.72 |
Nucleolus | 0.48 | 0.46 | 0.15 | -0.72 | -0.80 | 0.71 | 1.07 | -0.78 | -6.71 | -8.84 | 0.70 | 0.85 | -0.51 | -3.90 | -5.19 |
Nucleus | 0.04 | -4.65 | -8.66 | -9.32 | -0.58 | 0.01 | 0.17 | -12.26 | -12.80 | -12.66 | 0.03 | -4.40 | -14.70 | -15.63 | -7.03 |
Peroxisomes | 1.06 | 2.20 | -0.28 | -2.00 | -3.71 | -3.90 | 3.51 | -6.41 | -2.47 | -8.48 | -2.61 | 3.37 | -5.65 | -3.59 | -8.93 |
Vacuole | -0.28 | -9.13 | -15.28 | -16.39 | 0.34 | 0.08 | -5.05 | -24.20 | -24.21 | -15.79 | -1.57 | -10.28 | -29.16 | -28.83 | -6.76 |
Vacuole Periphery | 1.24 | 1.75 | 1.34 | 0.67 | -4.42 | -1.02 | 1.83 | -2.60 | -2.21 | -3.21 | 0.69 | 2.01 | 0.07 | -1.27 | -4.52 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|