ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL115C is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Cytoplasm 0.94 0.97 0.97 0.97 0.81 0.76 0.69 0.73 0.73 0.68 0.74 0.71 0.99 0.99 1.0 – – 0.56 0.4 0.45 0.28 0.43 0.43 0.38
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Mitochondria 0.07 0.08 0 0 0.05 0.18 0.19 0.16 0.2 0.26 0.21 0.25 0 0 0 – – 0.05 0 0 0.07 0 0 0.05
Nucleus 0 0 0 0 0 0 0.08 0.07 0 0.07 0 0.05 0 0 0 – – 0.13 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.28 0.28 0.32 0.22 0.28 0.29 0.26 0.28 0 0 0 – – 0.54 0.5 0.45 0.52 0.45 0.43 0.44
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 – – 0 0 1 4 0 2 2
Bud 0 1 0 1 0 1 3 8 3 3 4 5 1 1 0 – – 0 0 1 2 5 11 18
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 2 3 1 – – 0 0 1 2 1 5 7
Bud Site 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 – – 0 – – – – – –
Cell Periphery 0 0 0 0 0 1 0 1 1 0 0 0 5 5 2 – – 0 0 0 0 1 2 2
Cytoplasm 64 231 280 252 283 255 355 393 361 420 293 383 412 412 383 – – 107 37 105 79 157 247 248
Endoplasmic Reticulum 0 1 0 0 1 0 1 0 1 1 1 0 1 1 1 – – 8 0 0 5 3 5 6
Endosome 0 0 0 0 4 3 0 1 0 0 0 0 1 1 0 – – 2 2 3 7 3 6 7
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 2 2 1 2
Mitochondria 5 18 9 8 18 59 98 86 101 158 83 134 0 0 0 – – 9 4 8 18 11 25 34
Nucleus 0 0 0 2 8 15 40 36 20 41 12 25 3 3 1 – – 25 0 0 1 2 1 2
Nuclear Periphery 0 0 2 0 0 2 5 4 6 4 2 2 0 0 0 – – 0 0 0 0 0 2 0
Nucleolus 0 1 0 0 0 0 2 2 0 1 0 3 0 0 0 – – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 1 0 0 0
SpindlePole 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 – – 0 0 1 2 1 4 12
Vac/Vac Membrane 1 3 8 6 97 93 162 120 138 181 101 153 1 2 0 – – 103 47 105 148 162 249 289
Unique Cell Count 68 239 290 261 350 334 514 541 495 617 396 539 417 417 384 190 96 234 284 365 577 652
Labelled Cell Count 70 255 299 269 412 429 666 652 631 809 496 705 426 428 389 254 96 234 284 365 577 652


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 3.3 3.7 3.6 4.0 3.6 3.5 3.9 3.8 3.5 3.7 3.6 5.0 5.0 5.2 3.9 4.7 5.7 5.3 5.1 5.4
Std Deviation (1e-4) 0.3 0.5 1.3 1.4 1.5 1.6 0.8 1.4 1.2 1.1 1.2 1.1 1.1 1.1 1.4 1.1 1.3 1.4 1.6 1.4 1.7
Intensity Change (Log2) – – – -0.05 0.09 -0.04 -0.08 0.04 0.01 -0.11 -0.02 -0.07 0.42 0.42 0.48 0.07 0.33 0.6 0.51 0.46 0.52


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – 0
Bud Neck – – – – – – – – – – – – – – 0
Bud Site – – – – – – – – – – – – – – 0
Cell Periphery – – – – – – – – – – – – – – 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – 0
Endosome – – – – – – – – – – – – – – 0
Golgi – – – – – – – – – – – – – – 0
Mitochondria – – – – – – – – – – – – – – 0
Nuclear Periphery – – – – – – – – – – – – – – 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – 0
Peroxisomes – – – – – – – – – – – – – – 0
SpindlePole – – – – – – – – – – – – – – 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – 0
Cytoplasm – – – – – – – – – – – – – – 0
Nucleus – – – – – – – – – – – – – – 0
Vacuole – – – – – – – – – – – – – – 13.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.1787 0.1366 0.1002 -0.0728 0.0092 0.1748 -1.6154 -0.5479 -1.122 -1.1781 -1.2088 -0.9087 -1.7095 -0.7446 -1.4037 -1.5955 -1.7758 -1.3988
Actin 0.019 0.0047 0.0137 0.0003 0.0234 0.001 0.0159 0.0006 0.0002 0.0002 0.0114 0.004 0.0535 0.0001 0.0112 0 0 0.0028
Bud 0.0024 0.0084 0.0027 0.0008 0.0083 0.0015 0.0001 0.0019 0.0005 0.0017 0.0002 0.0011 0.0003 0.0004 0.0004 0.0001 0.0003 0.0002
Bud Neck 0.0045 0.0012 0.0021 0.0005 0.0041 0.0046 0.0013 0.0003 0.0051 0.0013 0.0004 0.0022 0.0003 0.0004 0.0004 0.0001 0.0003 0.0005
Bud Periphery 0.0047 0.0125 0.0026 0.0019 0.0045 0.0034 0.0002 0.0015 0.0017 0.0034 0.0005 0.003 0.0006 0.0005 0.0005 0.0002 0.0004 0.0004
Bud Site 0.0104 0.0149 0.0035 0.0009 0.0044 0.0007 0.0006 0.0118 0.0024 0.0025 0.0006 0.0013 0.002 0.0014 0.0043 0.0001 0.0002 0.0001
Cell Periphery 0.0007 0.001 0.0006 0.0002 0.0014 0.0004 0.0008 0.0004 0.0007 0.0006 0.0004 0.0005 0.0005 0.0005 0.0004 0.0001 0.0001 0.0001
Cytoplasm 0.1038 0.1443 0.0988 0.1287 0.1056 0.1656 0.0609 0.2071 0.1597 0.1422 0.2458 0.18 0.1244 0.2213 0.1554 0.205 0.1482 0.2593
Cytoplasmic Foci 0.0535 0.0522 0.0639 0.0837 0.0581 0.0492 0.0199 0.0325 0.0292 0.1099 0.031 0.043 0.0323 0.0418 0.0534 0.0586 0.1501 0.0402
Eisosomes 0.0003 0.0001 0.0003 0.0001 0.0003 0.0001 0.0005 0.0002 0.0002 0.0001 0.0014 0.0002 0.0012 0.0001 0.0002 0 0 0.0001
Endoplasmic Reticulum 0.0076 0.0028 0.0028 0.0031 0.0045 0.0028 0.0019 0.0066 0.0033 0.0029 0.0038 0.0013 0.0047 0.0049 0.0054 0.0032 0.0066 0.0023
Endosome 0.0885 0.0675 0.0943 0.1171 0.0961 0.0816 0.0373 0.0259 0.0447 0.0935 0.0669 0.0687 0.0309 0.1008 0.1204 0.1104 0.1301 0.0628
Golgi 0.0046 0.0026 0.0034 0.0046 0.0099 0.0017 0.0014 0.0007 0.0008 0.0024 0.0112 0.0016 0.0046 0.0014 0.0024 0.0013 0.0015 0.0009
Lipid Particles 0.0143 0.0046 0.0115 0.022 0.021 0.0105 0.0124 0.0051 0.0033 0.0102 0.0763 0.0016 0.0091 0.0047 0.0133 0.0068 0.0091 0.0036
Mitochondria 0.0057 0.0059 0.0058 0.0048 0.0302 0.011 0.001 0.0014 0.0061 0.0037 0.0272 0.0062 0.036 0.0022 0.0131 0.0017 0.0047 0.0106
None 0.5357 0.5571 0.5735 0.4905 0.43 0.5367 0.806 0.6788 0.6563 0.4899 0.4309 0.5852 0.6613 0.4982 0.5295 0.5718 0.4939 0.5332
Nuclear Periphery 0.0189 0.0019 0.0036 0.0033 0.0147 0.0034 0.0012 0.0017 0.0028 0.002 0.0025 0.0007 0.0016 0.0036 0.0043 0.0032 0.0106 0.0015
Nucleolus 0.0038 0.0051 0.0042 0.0033 0.0055 0.0016 0.0015 0.0007 0.0014 0.0014 0.0007 0.0006 0.0005 0.0016 0.0013 0.0006 0.0006 0.0003
Nucleus 0.0148 0.0106 0.0088 0.0076 0.0107 0.0099 0.002 0.0015 0.0036 0.0048 0.0025 0.0021 0.001 0.0033 0.0032 0.0019 0.0032 0.0016
Peroxisomes 0.0074 0.0026 0.008 0.0102 0.022 0.0057 0.0034 0.0007 0.0012 0.0045 0.0151 0.003 0.0078 0.0007 0.0028 0.0006 0.001 0.0004
Punctate Nuclear 0.0062 0.0025 0.0041 0.0039 0.0059 0.006 0.0036 0.0023 0.0013 0.0113 0.0018 0.0005 0.0008 0.001 0.0013 0.001 0.0058 0.0014
Vacuole 0.0837 0.0914 0.0829 0.1024 0.1285 0.0938 0.0266 0.0168 0.0716 0.1032 0.065 0.0837 0.0251 0.0975 0.0683 0.0294 0.0286 0.0705
Vacuole Periphery 0.0094 0.006 0.0089 0.0103 0.011 0.009 0.0014 0.0016 0.0039 0.0082 0.0042 0.0094 0.0015 0.0136 0.0085 0.0038 0.0048 0.0072

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.2559 7.1366 7.5652 13.2687 14.7867 13.1081 12.4988 14.6105 19.0664 18.2506
Translational Efficiency 1.7333 1.565 1.3984 0.802 0.876 2.339 1.0667 0.9551 1.0093 1.0623

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
72 847 263 1012 1434 653 345 1591 1506 1500 608 2603

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 872.59 669.99 835.20 777.11 637.20 692.84 790.16 782.63 648.45 679.94 809.64 780.48
Standard Deviation 158.12 120.19 93.69 104.43 79.01 81.85 110.34 104.16 98.30 105.84 105.84 104.30
Intensity Change Log 2 — -0.381164 -0.063182 -0.167185 — 0.120776 0.310399 0.296584 — -0.147742 0.106411 0.046958

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.003957 0.000168 0.000315 0.000334 0.000123 0.000245 0.000307 0.000199 0.000306 0.000201 0.000310 0.000251
Bud Neck 0.006002 0.026146 0.002657 0.006743 0.022350 0.055719 0.003406 0.009302 0.021568 0.039020 0.003082 0.008307
Bud Site 0.040839 0.012462 0.014663 0.034634 0.005899 0.012160 0.026252 0.025804 0.007570 0.012331 0.021239 0.029237
Cell Periphery 0.000378 0.000100 0.000188 0.000185 0.000124 0.000178 0.000763 0.000099 0.000136 0.000134 0.000514 0.000133
Cytoplasm 0.200856* 0.442055* 0.227367* 0.458034* 0.436124* 0.279723* 0.400894* 0.525096* 0.424876* 0.371386* 0.325832* 0.499023*
Cytoplasmic Foci 0.078847 0.232202* 0.027405 0.034574 0.264565* 0.277420* 0.020364 0.027540 0.255686* 0.251887* 0.023410 0.030274
Eisosomes 0.000119 0.000121 0.000094 0.000063 0.000222 0.000261 0.000058 0.000039 0.000217 0.000182 0.000074 0.000048
Endoplasmic Reticulum 0.002507 0.000447 0.003971 0.001634 0.000518 0.000876 0.009886 0.000792 0.000613 0.000634 0.007327 0.001119
Endosome 0.034684 0.034431 0.004974 0.006806 0.028691 0.075572 0.002905 0.007875 0.028978 0.052341 0.003800 0.007459
Golgi 0.008145 0.003392 0.000098 0.003408 0.002950 0.007411 0.004538 0.002780 0.003199 0.005142 0.002617 0.003024
Lipid Particles 0.002563 0.006497 0.003796 0.001483 0.009598 0.011968 0.002001 0.000941 0.009261 0.008879 0.002777 0.001152
Mitochondria 0.021433 0.004227 0.000498 0.001318 0.002823 0.017377 0.000422 0.004957 0.003713 0.009951 0.000455 0.003542
Mitotic Spindle 0.017086 0.005217 0.001249 0.019277 0.000738 0.003061 0.003480 0.039146 0.001520 0.004278 0.002515 0.031421
None 0.000637 0.003278 0.005796 0.002869 0.001631 0.001048 0.003891 0.005499 0.001584 0.002308 0.004715 0.004477
Nuclear Periphery 0.000970 0.000408 0.001849 0.001488 0.000369 0.000751 0.002087 0.001158 0.000397 0.000557 0.001984 0.001286
Nuclear Periphery Foci 0.001102 0.000557 0.001471 0.000968 0.000243 0.000206 0.001050 0.000629 0.000285 0.000404 0.001232 0.000761
Nucleolus 0.000601 0.001908 0.000964 0.000858 0.001047 0.001552 0.000341 0.001603 0.001026 0.001753 0.000611 0.001313
Nucleus 0.100217 0.090204 0.625899* 0.203424* 0.104354 0.069102 0.418239* 0.193765* 0.104156 0.081018 0.508066* 0.197520*
Peroxisomes 0.004687 0.009210 0.001090 0.001167 0.009026 0.021353 0.000226 0.002366 0.008818 0.014496 0.000600 0.001900
Vacuole 0.466741* 0.124324 0.075422 0.218610* 0.107348 0.159684 0.098515 0.144800 0.124530 0.139717 0.088526 0.173496
Vacuole Periphery 0.007627 0.002643 0.000232 0.002122 0.001256 0.004334 0.000377 0.005611 0.001561 0.003379 0.000314 0.004255

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.47 1.38 1.42 -3.06 1.75 -2.32 -3.99 -2.56 1.30 3.09 0.87 -0.48 0.74 -0.65 3.60
Bud Neck -7.11 1.22 -0.38 8.30 -3.11 -7.00 11.27 8.88 10.60 -2.77 -6.21 11.94 9.56 13.16 -4.13
Bud Site 2.04 1.88 1.13 -2.91 -1.67 -3.06 -2.36 -3.68 -0.38 0.84 -3.04 -2.11 -4.81 -2.29 -0.42
Cell Periphery 2.46 1.05 1.95 -3.03 3.23 -3.46 -1.62 6.94 6.98 1.84 -0.14 -1.85 3.83 3.93 2.11
Cytoplasm -7.93 -0.37 -6.60 3.27 -11.90 13.06 6.84 0.21 -12.80 -6.68 5.43 12.09 0.65 -5.34 -12.37
Cytoplasmic Foci -12.12 3.61 3.60 27.01 -0.34 -1.42 42.87 43.54 31.87 -1.51 0.44 41.56 42.54 41.25 -1.01
Eisosomes -1.08 -0.72 2.51 8.56 5.50 -1.80 12.12 15.60 11.00 5.98 2.30 10.49 15.04 13.68 8.00
Endoplasmic Reticulum 2.63 -4.72 0.19 -10.74 7.44 -1.53 -2.85 -9.85 -1.76 2.56 -0.34 -4.32 -11.32 -7.70 3.68
Endosome -1.37 4.10 4.32 11.88 1.31 -9.93 15.07 14.15 16.14 -1.88 -8.16 14.81 14.68 19.65 -0.27
Golgi 0.75 1.77 1.24 1.60 -1.71 -3.02 0.39 3.02 4.53 0.54 -2.30 1.44 2.77 4.89 0.01
Lipid Particles -4.56 -3.28 1.63 6.31 6.32 -1.71 9.27 11.14 9.76 4.88 0.48 7.92 10.91 11.59 7.93
Mitochondria 1.25 1.67 1.62 3.27 -1.68 -5.10 4.74 0.87 5.31 -2.86 -4.33 4.65 2.40 5.88 -3.21
Mitotic Spindle 1.53 1.91 0.66 -3.05 -5.28 -2.22 -2.49 -7.98 -6.74 -5.32 -1.87 -1.39 -8.75 -6.75 -7.35
None -4.30 -4.44 -5.62 1.00 2.13 1.22 -1.71 -5.20 -5.95 -2.00 -1.41 -3.23 -5.11 -3.31 -0.40
Nuclear Periphery 1.61 -3.70 -2.79 -8.10 1.16 -1.49 -4.30 -20.14 -4.92 1.74 -1.32 -6.46 -17.44 -10.23 2.00
Nuclear Periphery Foci 2.84 -3.16 0.22 -3.95 3.91 1.35 -6.73 -4.16 -4.63 3.34 -2.11 -9.99 -7.01 -4.91 5.19
Nucleolus -4.72 -2.02 -0.78 4.52 1.47 -1.77 5.88 0.63 1.70 -1.71 -3.48 3.99 1.20 3.80 -1.07
Nucleus 0.06 -16.74 -4.98 -15.75 19.93 6.32 -20.96 -21.28 -24.09 11.69 4.87 -31.93 -23.75 -28.12 21.17
Peroxisomes -1.25 1.27 1.34 11.17 0.43 -5.02 7.88 6.89 9.61 -2.02 -3.60 7.64 7.26 13.19 -1.13
Vacuole 8.90 9.09 4.94 -15.67 -13.48 -6.17 -5.34 -17.64 -8.32 -5.51 -2.44 -2.15 -19.13 -17.09 -12.39
Vacuole Periphery 0.94 1.73 1.43 2.17 -1.76 -5.68 6.88 -1.43 2.88 -3.15 -4.66 6.38 -0.56 3.05 -3.61
ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL115C is not an essential gene
Localization
Cell Percentages vacuole (26%), cytoplasm (3%), mixed (48%)
Cell Cycle Regulation No
Subcompartmental Group N/A

YNL115C

YNL115C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ynl115c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available