Standard name
Human Ortholog
Description Ubiquitin-protein ligase (E3); controls septin dynamics and spindle position checkpoint (SPOC) with ligase Dma1p by regulating recruitment of Elm1p to bud neck; regulates levels of eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ortholog of human RNF8, similar to human Chfr; contains FHA and RING finger domains; DMA2 has a paralog, DMA1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.06 0.09 0.09 0 0 0 0 0 0
Cytoplasm 0.96 0.97 0.97 0.97 0.95 0.92 0.81 0.71 0.75 0.69 0.62 0.58 0.99 0.99 1.0 0.97 0.96 0.97 0.93 0.9 0.81 0.91 0.87 0.81
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0 0.1 0.1 0.32 0.36 0.45 0.48 0.62 0.65 0.67 0 0 0 0 0 0 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 3 0 1 4 4 2 2 7 5
Bud 3 3 1 0 0 5 3 12 5 7 5 3 0 0 0 1 0 0 0 2 1 0 9 9
Bud Neck 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 4 2 3 4 4
Bud Site 0 0 0 0 0 0 0 0 3 0 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 9 2 3 5 3 4 4 4 9 2 7 1 9 5 37 23 29 1 1 2 0 1 2
Cytoplasm 411 465 189 127 170 171 185 109 155 132 128 116 267 479 440 599 243 326 383 442 163 148 328 327
Endoplasmic Reticulum 0 7 1 1 1 1 0 0 1 1 0 1 2 1 0 31 15 13 1 1 4 0 3 4
Endosome 0 0 1 0 0 1 3 1 0 0 0 0 0 0 0 2 2 1 2 8 4 1 1 3
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 4 1 6 0 0 2 0
Mitochondria 27 9 4 13 18 60 81 69 99 119 133 134 0 1 0 6 3 7 5 10 11 0 5 9
Nucleus 2 0 0 0 1 0 1 2 0 0 0 0 0 0 0 6 0 1 1 0 0 0 2 1
Nuclear Periphery 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 0 2 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 0 0 0 0 2 0 0 1 0 0 0 3 0 0 0 2 1 0 1 3
Vac/Vac Membrane 1 2 0 0 0 0 3 0 3 2 0 4 0 1 0 3 4 2 4 3 3 2 4 18
Unique Cell Count 426 478 194 131 179 185 228 154 207 192 205 200 269 482 440 617 252 336 414 492 202 163 378 402
Labelled Cell Count 448 498 201 144 195 241 280 197 272 272 270 269 270 491 445 692 291 384 414 492 202 163 378 402


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 4.9 4.8 4.7 4.7 4.3 4.1 4.1 4.0 3.5 3.6 3.5 5.8 5.2 5.3 6.9 8.1 8.0 4.7 5.0 5.6
Std Deviation (1e-4) 0.6 0.6 0.9 0.9 1.1 1.4 1.7 1.9 1.9 0.9 1.0 1.3 1.6 1.4 1.4 1.3 1.4 1.5 1.1 1.5 1.5
Intensity Change (Log2) – – – -0.03 -0.01 -0.16 -0.22 -0.22 -0.27 -0.46 -0.43 -0.45 0.28 0.13 0.15 0.53 0.76 0.75 -0.01 0.05 0.23

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP70002468WT3HU80HU120HU16002468WT3rpd3Δ_1rpd3Δ_2rpd3Δ_302468WT1AF100AF140AF18002468
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 2.8 3.7 3.6
Cytoplasm -0.3 -1.2 -2.2 -5.3 -7.0 -6.5 -7.5 -8.6 -9.4 1.6 2.1 3.4 -0.2 -0.6 -0.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 2.8 3.1 2.3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.7672 2.3587 2.3093 2.1312 2.0053 2.3984 3.9912 3.8326 4.0932 4.5256 3.7059 3.7726 4.6734 4.4024 4.2276 4.0846 4.6986 4.1553
Actin 0.0103 0.0056 0.0039 0.0002 0.0082 0.0066 0.064 0.0005 0.0195 0.0039 0.0086 0.0051 0.0506 0.0167 0.0157 0.0002 0.028 0.0173
Bud 0.0004 0.0012 0.0007 0.0023 0.0053 0.0004 0.001 0.0004 0.0026 0.0013 0.0004 0.0004 0.0034 0.0023 0.0041 0.0001 0.0004 0.0014
Bud Neck 0.0003 0.0006 0.0021 0.0004 0.0005 0.0015 0.0082 0.0001 0.0008 0.0018 0.0009 0.0022 0.0074 0.0002 0.0046 0.0001 0.0008 0.0014
Bud Periphery 0.0007 0.0013 0.0008 0.0019 0.0276 0.0006 0.0018 0.0006 0.0035 0.0022 0.0008 0.0008 0.0119 0.0049 0.004 0.0001 0.0008 0.0024
Bud Site 0.0014 0.0161 0.0135 0.0035 0.0007 0.0013 0.009 0.0015 0.0174 0.0214 0.0007 0.0002 0.0094 0.0045 0.0141 0 0.0007 0.0004
Cell Periphery 0.0005 0.0023 0.0009 0.0002 0.0003 0.0003 0.0007 0.0003 0.0005 0.0013 0.0002 0.0001 0.0018 0.0005 0.0004 0.0001 0.0001 0.0002
Cytoplasm 0.3147 0.408 0.402 0.5204 0.4261 0.535 0.3679 0.4559 0.4286 0.4282 0.4574 0.5448 0.2089 0.4682 0.3516 0.4625 0.4265 0.4862
Cytoplasmic Foci 0.0074 0.0105 0.0108 0.0711 0.0315 0.012 0.0357 0.0053 0.021 0.0072 0.0075 0.0203 0.02 0.0036 0.0131 0.0026 0.0094 0.0118
Eisosomes 0.0004 0.0004 0.0002 0.0001 0.0002 0.0002 0.0006 0.0001 0.0004 0.0002 0.0001 0.0001 0.0025 0.0002 0.0004 0.0001 0.0002 0.0002
Endoplasmic Reticulum 0.0032 0.0042 0.0043 0.0037 0.0028 0.0027 0.0097 0.0021 0.0038 0.0017 0.0025 0.0018 0.0146 0.0017 0.0026 0.0013 0.0015 0.0018
Endosome 0.0103 0.002 0.0081 0.0069 0.017 0.0054 0.0239 0.0018 0.0187 0.0075 0.0534 0.0024 0.0398 0.0041 0.0205 0.001 0.0091 0.0028
Golgi 0.0047 0.0006 0.003 0.0003 0.002 0.0011 0.0089 0.0003 0.0039 0.0035 0.0122 0.0004 0.0159 0.0114 0.0073 0.0001 0.0044 0.0015
Lipid Particles 0.0076 0.0005 0.0031 0.0007 0.001 0.0009 0.0107 0.0002 0.004 0.0031 0.0105 0.0004 0.0219 0.0001 0.0067 0.0001 0.0062 0.0016
Mitochondria 0.0047 0.0004 0.0049 0.0003 0.0065 0.0014 0.0052 0.0003 0.005 0.0064 0.008 0.0002 0.0071 0.0014 0.0076 0.0001 0.0017 0.0005
None 0.5994 0.5412 0.5159 0.3766 0.4529 0.4253 0.405 0.5275 0.4472 0.501 0.4117 0.4155 0.5225 0.4765 0.5223 0.5304 0.4941 0.4664
Nuclear Periphery 0.0015 0.0005 0.001 0.0008 0.0008 0.0008 0.0114 0.0007 0.0027 0.0004 0.0077 0.0006 0.0247 0.0005 0.0012 0.0003 0.0016 0.0005
Nucleolus 0.0017 0.0002 0.0002 0.0005 0.0002 0.0001 0.0013 0.0001 0.0007 0.0006 0.0005 0.0001 0.0013 0.0002 0.0004 0.0001 0.0002 0.0001
Nucleus 0.0021 0.0013 0.0012 0.0014 0.0009 0.0008 0.0078 0.0007 0.0012 0.001 0.0016 0.0005 0.0056 0.0013 0.0013 0.0005 0.0007 0.0005
Peroxisomes 0.0239 0.0004 0.0205 0.0005 0.0121 0.0011 0.0117 0.0001 0.0133 0.001 0.0011 0.0021 0.0127 0.0001 0.0151 0 0.0081 0.0018
Punctate Nuclear 0.0022 0.0011 0.0007 0.0055 0.0006 0.0008 0.0115 0.0004 0.0027 0.0004 0.0041 0.0012 0.012 0.0008 0.0018 0.0002 0.0041 0.0009
Vacuole 0.002 0.0016 0.0018 0.0024 0.0023 0.0014 0.0027 0.0009 0.0019 0.0052 0.0055 0.0007 0.004 0.0006 0.0038 0.0003 0.0012 0.0004
Vacuole Periphery 0.0006 0.0001 0.0003 0.0003 0.0006 0.0003 0.0012 0.0001 0.0007 0.0007 0.0045 0.0001 0.002 0.0001 0.0014 0 0.0003 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.3395 14.4593 15.8609 13.8051 13.2211 16.1659 19.3871 18.4477 15.676 19.442
Translational Efficiency 1.2003 1.649 1.0505 0.8461 1.6913 1.4214 1.1333 1.044 1.2126 1.1613

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
862 1342 1902 1650 1758 1269 138 2295 2620 2611 2040 3945

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 649.76 730.22 852.49 886.24 697.64 698.29 946.82 955.06 681.89 714.70 858.87 926.28
Standard Deviation 73.77 94.81 114.69 136.15 86.70 98.08 126.08 132.47 85.68 97.72 117.90 138.25
Intensity Change Log 2 — 0.168424 0.391776 0.447791 — 0.001344 0.440607 0.453109 — 0.084333 0.417266 0.450546

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000087 0.000665 0.000278 0.001221 0.000189 0.000487 0.000263 0.001875 0.000155 0.000578 0.000277 0.001601
Bud Neck 0.012741 0.034168 0.006057 0.017055 0.020402 0.039408 0.002718 0.018032 0.017882 0.036715 0.005831 0.017623
Bud Site 0.005830 0.032282 0.010740 0.072824 0.005722 0.024828 0.012260 0.071628 0.005757 0.028659 0.010843 0.072128
Cell Periphery 0.000200 0.000142 0.000146 0.000275 0.000227 0.000174 0.000179 0.000168 0.000218 0.000157 0.000149 0.000213
Cytoplasm 0.597581 0.468904 0.625086 0.608874 0.507044 0.399312 0.538670 0.607069 0.536831 0.435081 0.619240 0.607824
Cytoplasmic Foci 0.192936 0.225563 0.028254 0.025925 0.216126 0.266537 0.030898 0.019305 0.208496 0.245477 0.028433 0.022074
Eisosomes 0.000219 0.000123 0.000047 0.000044 0.000324 0.000206 0.000068 0.000034 0.000290 0.000164 0.000049 0.000039
Endoplasmic Reticulum 0.000806 0.000761 0.001432 0.000924 0.001455 0.000705 0.002231 0.000670 0.001241 0.000734 0.001486 0.000776
Endosome 0.009363 0.025515 0.003598 0.004416 0.018501 0.043501 0.004191 0.006477 0.015494 0.034257 0.003638 0.005615
Golgi 0.001523 0.006817 0.000382 0.001868 0.003824 0.010776 0.000123 0.006050 0.003067 0.008741 0.000364 0.004301
Lipid Particles 0.005178 0.003637 0.001095 0.000753 0.009154 0.007318 0.001509 0.000765 0.007846 0.005426 0.001123 0.000760
Mitochondria 0.000894 0.009973 0.000320 0.005652 0.003587 0.016922 0.000142 0.003367 0.002701 0.013351 0.000308 0.004323
Mitotic Spindle 0.000905 0.009497 0.003145 0.040636 0.002081 0.006436 0.002959 0.070353 0.001694 0.008009 0.003133 0.057924
None 0.003760 0.002962 0.005262 0.003402 0.004128 0.002853 0.007163 0.002188 0.004007 0.002909 0.005390 0.002696
Nuclear Periphery 0.000351 0.000304 0.000887 0.000455 0.000404 0.000247 0.000911 0.000693 0.000386 0.000277 0.000888 0.000594
Nuclear Periphery Foci 0.000907 0.000764 0.001083 0.000941 0.000314 0.000346 0.005153 0.000769 0.000509 0.000561 0.001358 0.000841
Nucleolus 0.000593 0.001359 0.000360 0.000287 0.000745 0.001878 0.000160 0.000437 0.000695 0.001611 0.000347 0.000375
Nucleus 0.107095 0.075372 0.197229 0.077899 0.115046 0.057851 0.120912 0.055074 0.112430 0.066856 0.192067 0.064621
Peroxisomes 0.002682 0.015323 0.000517 0.001862 0.003731 0.015540 0.000286 0.003671 0.003386 0.015429 0.000501 0.002914
Vacuole 0.055779 0.083475 0.113931 0.132866 0.085653 0.100444 0.269083 0.126967 0.075824 0.091722 0.124426 0.129434
Vacuole Periphery 0.000571 0.002395 0.000153 0.001819 0.001343 0.004231 0.000121 0.004407 0.001089 0.003287 0.000150 0.003325

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.25 -5.65 -7.19 -1.25 -4.87 -2.53 -3.61 -4.48 -2.20 -2.78 -5.87 -3.73 -6.93 -2.45 -5.32
Bud Neck -7.67 4.28 0.70 8.71 -4.34 -6.44 10.00 5.63 10.65 -6.51 -9.10 9.57 5.24 13.78 -6.01
Bud Site -10.70 -2.64 -10.83 -1.40 -9.27 -8.90 -1.64 -12.87 -3.31 -3.86 -14.07 -3.48 -16.86 -3.14 -12.70
Cell Periphery 4.89 5.59 0.09 -2.97 -3.21 4.36 0.98 9.92 5.53 4.22 6.94 8.38 6.53 0.97 -0.37
Cytoplasm 11.75 5.08 7.80 -4.52 3.20 10.85 4.75 -4.12 -14.47 -6.25 13.75 0.12 0.79 -13.34 0.57
Cytoplasmic Foci -3.85 28.98 29.51 34.07 1.53 -6.67 33.26 42.82 40.12 2.08 -6.59 48.52 51.70 52.38 3.93
Eisosomes 6.24 11.45 11.82 13.27 1.70 3.21 7.13 8.83 9.52 7.30 5.25 10.48 11.11 13.37 5.64
Endoplasmic Reticulum 0.13 -6.12 -1.19 -2.33 8.58 2.33 -4.22 2.83 0.18 7.72 2.29 -4.82 2.04 -0.86 12.05
Endosome -8.55 7.20 8.11 13.25 1.49 -9.20 11.40 12.77 16.44 1.93 -10.95 13.59 14.73 20.92 1.19
Golgi -6.86 3.72 2.16 8.50 -1.46 -5.93 9.26 2.62 7.08 -3.32 -7.98 8.98 3.62 9.98 -3.27
Lipid Particles 2.80 8.20 8.45 7.51 1.42 2.17 11.80 13.93 10.66 5.42 4.51 15.37 16.01 12.95 3.41
Mitochondria -6.38 4.84 -1.95 4.72 -2.70 -6.32 4.70 2.72 7.75 -5.65 -8.13 4.82 1.42 9.14 -4.49
Mitotic Spindle -4.96 -2.73 -7.81 -3.79 -6.82 -2.51 -1.68 -10.75 -8.49 -5.55 -4.93 -1.85 -13.08 -9.01 -12.09
None 0.77 -0.45 0.77 -0.28 1.46 1.17 0.03 1.74 0.51 1.03 1.37 -0.23 1.83 0.19 2.03
Nuclear Periphery 0.25 -7.43 -4.39 -6.54 7.12 3.39 -8.09 -6.79 -9.54 2.99 1.55 -14.92 -8.43 -10.44 6.66
Nuclear Periphery Foci 0.19 -0.75 -0.44 -1.16 1.54 -1.05 -6.57 -6.18 -4.90 6.01 -0.26 -5.04 -1.97 -1.86 6.41
Nucleolus -2.90 0.87 2.62 3.90 0.50 -3.35 7.20 3.05 4.06 -2.58 -4.34 1.67 4.24 5.53 0.10
Nucleus 6.08 -20.32 -0.32 -6.92 21.07 13.31 -1.24 5.27 -9.92 3.01 13.35 -20.96 3.98 -11.11 24.45
Peroxisomes -9.67 3.80 2.90 12.71 -3.12 -8.96 11.61 4.38 10.38 -3.06 -13.37 10.10 5.69 15.98 -3.37
Vacuole -7.44 -19.52 -29.57 -23.92 -13.38 -3.24 -12.60 -26.86 -22.30 6.01 -5.55 -18.97 -38.56 -32.96 -12.70
Vacuole Periphery -6.83 6.99 -0.61 4.89 -2.60 -6.52 7.25 -0.42 5.04 -3.91 -8.22 8.35 -0.33 6.38 -4.63
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Ubiquitin-protein ligase (E3); controls septin dynamics and spindle position checkpoint (SPOC) with ligase Dma1p by regulating recruitment of Elm1p to bud neck; regulates levels of eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ortholog of human RNF8, similar to human Chfr; contains FHA and RING finger domains; DMA2 has a paralog, DMA1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (78%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Dma2

Dma2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dma2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available