Standard name
Human Ortholog
Description Protein required for uridine thiolation of Lys(UUU) and Glu(UUC) tRNAs; required for the thiolation of uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Cytoplasm 0.96 0.99 0.99 0.89 0.99 0.96 0.76 0.84 0.73 0.72 0.55 0.57 1.0 0.99 1.0 0.78 0.8 0.85 0.96 0.94 0.91 0.92 0.94 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.14 0.08 0.05 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Mitochondria 0 0 0 0.17 0 0.26 0.48 0.33 0.5 0.56 0.66 0.64 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0.06 0.08 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 2 0 0 1 0 0 0 1
Bud 0 1 0 0 0 1 1 3 2 5 2 1 2 1 1 0 0 0 0 2 0 0 0 0
Bud Neck 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 8 1 2 2 2 0 0 0 2 5 5 3 2 4 6 9 3 0 0 0 0 0 0
Cytoplasm 301 386 68 48 93 98 99 139 48 92 80 74 180 190 194 138 123 94 294 372 79 109 135 162
Endoplasmic Reticulum 2 3 1 0 0 0 0 1 0 1 3 1 0 0 0 7 4 8 1 2 0 2 0 3
Endosome 0 0 0 1 0 0 1 1 0 1 2 0 1 0 0 25 13 6 4 3 0 1 2 1
Golgi 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 4 5 0 1 1 0 0 0
Mitochondria 10 0 2 9 3 27 62 54 33 71 97 82 1 1 0 2 4 3 1 1 0 0 0 1
Nucleus 1 0 0 0 0 0 1 0 0 0 0 2 0 0 2 0 4 1 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 3 3 0 0 0 0 0 0 0 7 1 3 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 9 9 1 0 0 0 0 1 0
Vac/Vac Membrane 1 1 0 3 0 0 4 2 0 3 1 2 0 0 0 10 12 5 0 7 0 0 0 2
Unique Cell Count 312 390 69 54 94 102 130 165 66 127 146 129 180 191 194 176 153 111 308 397 87 118 144 177
Labelled Cell Count 318 399 72 63 98 129 172 204 83 176 192 167 187 194 201 205 185 129 308 397 87 118 144 177


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 7.3 6.1 6.2 5.5 4.6 3.9 4.5 4.4 3.6 4.1 3.8 6.7 6.2 6.2 8.2 9.3 9.7 6.2 6.3 7.1
Std Deviation (1e-4) 0.6 1.0 1.3 2.1 1.7 0.9 1.3 2.1 2.2 0.7 1.7 1.2 0.9 1.2 1.2 1.5 1.7 2.4 1.5 1.5 1.2
Intensity Change (Log2) – – – 0.02 -0.17 -0.41 -0.65 -0.45 -0.49 -0.75 -0.59 -0.69 0.12 0.01 0.01 0.42 0.59 0.66 0.01 0.04 0.22

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP7000510WT3HU80HU120HU1600510WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30510WT1AF100AF140AF1800510
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.3 0.2 -0.9 -4.1 -3.1 -4.3 -4.5 -6.5 -6.1 1.6 0.8 1.7 -3.9 -3.6 -3.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 3.3 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.4048 4.2203 4.0131 3.7418 3.5164 3.7828 4.0807 4.3275 4.4279 4.2599 3.5415 4.2904 5.0475 5.8215 5.3925 4.7283 5.5245 5.2915
Actin 0.0102 0.0007 0.0012 0.0039 0.013 0.0019 0.0206 0.0029 0.02 0.0884 0.0605 0.0278 0.0675 0.0013 0.0131 0.0515 0.0042 0.0026
Bud 0.0012 0.0004 0.0022 0.0005 0.0013 0.0027 0.0007 0.0003 0.0029 0.0007 0.0004 0.0004 0.0015 0.0051 0.0059 0.0011 0.0003 0.0049
Bud Neck 0.0054 0.0002 0.0014 0.0004 0.001 0.0027 0.0128 0.0002 0.0012 0.0007 0.0006 0.0016 0.0218 0.0004 0.0028 0.0078 0.0005 0.0005
Bud Periphery 0.002 0.0003 0.0016 0.0004 0.0013 0.0032 0.0007 0.0002 0.0024 0.0014 0.0004 0.0006 0.0018 0.0025 0.0053 0.0008 0.0001 0.0021
Bud Site 0.0019 0.0012 0.0055 0.0003 0.0104 0.0013 0.0195 0.0031 0.0029 0.0039 0.0009 0.0007 0.0266 0.0156 0.0192 0.005 0.0015 0.0005
Cell Periphery 0.0008 0.0003 0.0005 0.0003 0.0002 0.0003 0.0005 0.0004 0.0003 0.0012 0.0002 0.0003 0.0007 0.0003 0.0006 0.0003 0.0001 0.0001
Cytoplasm 0.8084 0.9391 0.8854 0.8769 0.903 0.9005 0.6778 0.9367 0.8891 0.6952 0.8646 0.8581 0.6585 0.889 0.8162 0.7638 0.8563 0.9092
Cytoplasmic Foci 0.0166 0.0119 0.0169 0.0113 0.0195 0.0154 0.0301 0.0154 0.0219 0.0255 0.0097 0.0205 0.0445 0.0129 0.0228 0.0224 0.0361 0.0076
Eisosomes 0.0002 0 0.0001 0.0005 0.0001 0 0.0003 0 0 0.0069 0.0004 0.0004 0.0007 0 0.0003 0.001 0.0001 0
Endoplasmic Reticulum 0.017 0.0028 0.0064 0.0017 0.0048 0.0038 0.0134 0.0103 0.0031 0.0053 0.0032 0.0127 0.0129 0.0096 0.0049 0.0067 0.0026 0.0061
Endosome 0.0342 0.0097 0.0187 0.015 0.0258 0.0195 0.0598 0.011 0.0113 0.0375 0.0125 0.0155 0.0271 0.0076 0.0149 0.0293 0.0145 0.008
Golgi 0.0049 0.0011 0.0016 0.0005 0.0032 0.0039 0.0186 0.0028 0.007 0.0074 0.0226 0.0087 0.011 0.0015 0.0029 0.0069 0.002 0.0006
Lipid Particles 0.0023 0.0001 0.0004 0.0002 0.0007 0.002 0.0114 0.0004 0.0015 0.0054 0.0009 0.005 0.0176 0.0007 0.0023 0.0017 0.0003 0.0002
Mitochondria 0.0019 0.0007 0.0008 0.0004 0.0009 0.0015 0.0227 0.0003 0.0011 0.0156 0.0084 0.0049 0.0023 0.0002 0.0139 0.0042 0.0003 0.0005
None 0.0685 0.0257 0.0475 0.0762 0.0084 0.0266 0.0762 0.0104 0.0161 0.0912 0.0116 0.0282 0.0532 0.0482 0.0463 0.075 0.0745 0.0523
Nuclear Periphery 0.0041 0.0005 0.0011 0.0006 0.0007 0.0033 0.0054 0.0008 0.0004 0.0014 0.0005 0.0027 0.0037 0.0011 0.0019 0.0074 0.0007 0.0009
Nucleolus 0.0007 0 0.0002 0.0001 0.0001 0.0007 0.0008 0.0001 0 0.0008 0 0.0002 0.0012 0.0001 0.0012 0.0003 0.0001 0.0001
Nucleus 0.0057 0.001 0.0018 0.0011 0.001 0.0032 0.0031 0.0008 0.0005 0.0012 0.0005 0.0009 0.0022 0.0012 0.0024 0.0036 0.0007 0.0012
Peroxisomes 0.001 0.0003 0.0004 0.0006 0.0008 0.0023 0.0056 0.0003 0.0153 0.0057 0.0004 0.0074 0.0096 0.0003 0.008 0.0047 0.0016 0.0001
Punctate Nuclear 0.004 0.0004 0.0009 0.0002 0.0005 0.0006 0.009 0.0004 0.0004 0.0009 0.0002 0.0021 0.0317 0.0008 0.0106 0.0032 0.0011 0.0004
Vacuole 0.0079 0.0032 0.0049 0.0081 0.0026 0.0037 0.0098 0.0028 0.0023 0.003 0.0012 0.0011 0.0033 0.0015 0.004 0.0026 0.002 0.0016
Vacuole Periphery 0.001 0.0005 0.0007 0.0009 0.0006 0.0008 0.0014 0.0002 0.0004 0.0008 0.0004 0.0004 0.0006 0.0002 0.0007 0.0007 0.0003 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 26.0588 14.6426 18.7675 17.6255 23.1678 8.9295 15.9828 12.4905 10.3134 20.1875
Translational Efficiency 0.6027 1.0574 0.6547 0.5413 0.5825 1.2042 0.6864 0.5497 0.7725 0.6869

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
220 1540 1098 1768 2098 1385 628 230 2318 2925 1726 1998

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 740.43 817.19 983.48 998.43 783.82 854.55 997.95 1103.73 779.70 834.88 988.74 1010.55
Standard Deviation 78.73 130.01 117.66 125.30 95.93 110.99 109.04 148.71 95.28 122.80 114.81 132.54
Intensity Change Log 2 — 0.142308 0.409532 0.431298 — 0.124643 0.348445 0.493793 — 0.133251 0.378442 0.463773

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000187 0.000211 0.000086 0.001648 0.000052 0.000242 0.000126 0.000675 0.000065 0.000226 0.000101 0.001536
Bud Neck 0.009536 0.006521 0.001190 0.002333 0.002967 0.009995 0.000659 0.008078 0.003590 0.008166 0.000997 0.002995
Bud Site 0.007483 0.028317 0.007383 0.032655 0.002907 0.020889 0.006446 0.104232 0.003341 0.024800 0.007042 0.040894
Cell Periphery 0.000137 0.000124 0.000067 0.000049 0.000103 0.000101 0.000068 0.000082 0.000106 0.000113 0.000068 0.000053
Cytoplasm 0.637491 0.663176 0.746206 0.753465 0.721905 0.541671 0.785632 0.480954 0.713893 0.605643 0.760551 0.722095
Cytoplasmic Foci 0.181645 0.158480 0.050031 0.053085 0.137494 0.197913 0.042800 0.119554 0.141684 0.177152 0.047400 0.060737
Eisosomes 0.000111 0.000024 0.000021 0.000008 0.000055 0.000050 0.000020 0.000020 0.000060 0.000036 0.000021 0.000009
Endoplasmic Reticulum 0.000329 0.000749 0.000680 0.000285 0.000886 0.000879 0.000540 0.000286 0.000833 0.000811 0.000629 0.000285
Endosome 0.011993 0.018109 0.005874 0.005191 0.006371 0.035977 0.004926 0.026759 0.006905 0.026569 0.005529 0.007674
Golgi 0.006330 0.006812 0.000158 0.003737 0.001737 0.008904 0.000278 0.011852 0.002172 0.007802 0.000202 0.004671
Lipid Particles 0.004178 0.001483 0.000702 0.000983 0.002425 0.002003 0.000690 0.001657 0.002592 0.001729 0.000697 0.001060
Mitochondria 0.001284 0.001999 0.000094 0.002225 0.000702 0.003460 0.000050 0.001647 0.000758 0.002691 0.000078 0.002159
Mitotic Spindle 0.004730 0.003136 0.002583 0.074667 0.000127 0.009038 0.006136 0.076244 0.000564 0.005930 0.003876 0.074848
None 0.002465 0.002210 0.002536 0.001526 0.005967 0.000871 0.001812 0.001312 0.005635 0.001576 0.002273 0.001501
Nuclear Periphery 0.000152 0.000092 0.000311 0.000162 0.000135 0.000091 0.000207 0.000139 0.000136 0.000091 0.000273 0.000159
Nuclear Periphery Foci 0.000982 0.000594 0.002680 0.001114 0.000432 0.000215 0.004092 0.002682 0.000484 0.000415 0.003194 0.001294
Nucleolus 0.000640 0.000417 0.000177 0.000152 0.000359 0.000326 0.000117 0.000183 0.000385 0.000374 0.000155 0.000156
Nucleus 0.062500 0.015812 0.048406 0.013231 0.069534 0.013389 0.035812 0.004059 0.068866 0.014665 0.043823 0.012175
Peroxisomes 0.002021 0.002158 0.000199 0.006061 0.000549 0.002173 0.000195 0.016576 0.000689 0.002165 0.000198 0.007272
Vacuole 0.064963 0.085518 0.130352 0.045809 0.044386 0.146482 0.109080 0.139968 0.046339 0.114385 0.122612 0.056648
Vacuole Periphery 0.000842 0.004057 0.000265 0.001615 0.000909 0.005333 0.000314 0.003043 0.000902 0.004661 0.000283 0.001779

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.29 1.30 -2.48 -2.46 -2.86 -3.05 -3.64 -5.54 -2.90 -4.45 -4.74 -3.78 -3.17 -2.55 -3.00
Bud Neck 1.17 3.27 2.84 5.54 -5.09 -8.46 8.88 -2.32 4.21 -4.94 -7.27 7.48 2.46 9.65 -7.82
Bud Site -6.40 0.32 -4.78 2.33 -7.78 -13.28 -2.71 -6.61 -4.27 -6.05 -18.52 -3.22 -13.30 -0.81 -10.10
Cell Periphery 0.43 5.60 7.25 2.25 4.25 0.25 7.66 4.94 2.49 -1.47 -0.31 9.67 15.09 3.38 4.46
Cytoplasm -1.54 -3.87 -2.53 -1.78 2.24 21.00 -1.59 13.09 4.74 12.97 16.20 -0.47 7.27 -5.33 6.89
Cytoplasmic Foci 2.21 13.01 10.39 15.90 -6.12 -10.48 25.22 3.66 9.55 -7.20 -7.83 28.26 15.39 22.76 -8.74
Eisosomes 9.59 9.84 11.26 11.24 9.50 1.45 12.24 11.72 7.75 -0.60 8.83 15.82 21.57 15.16 10.49
Endoplasmic Reticulum -3.25 -4.04 1.64 4.39 5.60 0.06 2.30 4.22 4.00 4.18 0.17 1.43 4.41 5.63 6.06
Endosome -3.18 4.12 4.91 12.50 2.47 -17.58 3.61 -2.34 6.66 -3.05 -18.79 3.96 2.75 19.80 -0.37
Golgi -0.11 1.76 1.31 7.93 -3.95 -6.81 10.22 -0.70 1.92 -1.28 -8.44 5.61 0.43 8.07 -3.79
Lipid Particles 1.89 2.47 1.87 -0.08 -3.27 1.96 9.61 -0.14 -1.20 -4.73 3.57 9.40 3.31 0.41 -4.21
Mitochondria -0.96 2.04 0.50 1.99 -3.04 -3.24 9.90 0.64 3.24 -1.14 -4.10 8.31 -0.52 3.38 -3.23
Mitotic Spindle 0.47 0.76 -12.44 -14.88 -15.20 -4.63 -2.26 -6.68 -5.92 -6.27 -4.82 -2.64 -17.01 -15.43 -16.20
None 0.42 0.48 1.34 1.40 1.72 8.50 7.30 7.77 -0.24 2.02 7.24 6.62 7.69 -0.02 1.91
Nuclear Periphery 2.70 -6.90 -3.87 -6.69 2.89 2.31 -6.69 -5.60 -6.37 -2.20 3.19 -10.28 -6.40 -8.24 1.90
Nuclear Periphery Foci 0.70 -1.14 -0.36 -6.29 3.65 6.96 -7.82 -4.39 -4.53 -1.90 0.90 -11.61 -7.28 -8.39 2.54
Nucleolus 1.10 2.07 1.44 1.87 -4.50 0.49 13.58 5.82 3.83 -3.13 0.26 8.26 1.88 1.80 -5.34
Nucleus 7.52 1.48 7.04 -2.52 16.64 29.84 10.71 34.22 5.88 16.37 30.15 9.24 28.89 -3.35 19.91
Peroxisomes -0.22 3.02 -2.48 -3.21 -6.76 -10.00 10.39 -4.21 -3.33 -4.41 -11.05 7.73 -7.00 -4.27 -7.95
Vacuole -3.40 -12.47 -6.11 -3.89 8.85 -20.87 -14.30 -12.38 -6.49 -5.78 -21.57 -23.27 -18.38 -1.86 6.39
Vacuole Periphery -8.38 3.36 0.58 8.51 -2.19 -12.38 5.80 -0.00 5.91 -1.03 -14.89 8.57 0.90 12.58 -2.40
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Protein required for uridine thiolation of Lys(UUU) and Glu(UUC) tRNAs; required for the thiolation of uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae
Localization
Cell Percentages cytoplasm (94%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ncs2

Ncs2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ncs2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available