Standard name
Human Ortholog
Description Protein required for uridine thiolation of Lys(UUU) and Glu(UUC) tRNAs; required for the thiolation of uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Cytoplasm 0.96 0.99 0.99 0.89 0.99 0.96 0.76 0.84 0.73 0.72 0.55 0.57 1.0 0.99 1.0 0.78 0.8 0.85 0.96 0.94 0.91 0.92 0.94 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.14 0.08 0.05 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Mitochondria 0 0 0 0.17 0 0.26 0.48 0.33 0.5 0.56 0.66 0.64 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0.06 0.08 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 2 0 0 1 0 0 0 1
Bud 0 1 0 0 0 1 1 3 2 5 2 1 2 1 1 0 0 0 0 2 0 0 0 0
Bud Neck 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 8 1 2 2 2 0 0 0 2 5 5 3 2 4 6 9 3 0 0 0 0 0 0
Cytoplasm 301 386 68 48 93 98 99 139 48 92 80 74 180 190 194 138 123 94 294 372 79 109 135 162
Endoplasmic Reticulum 2 3 1 0 0 0 0 1 0 1 3 1 0 0 0 7 4 8 1 2 0 2 0 3
Endosome 0 0 0 1 0 0 1 1 0 1 2 0 1 0 0 25 13 6 4 3 0 1 2 1
Golgi 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 4 5 0 1 1 0 0 0
Mitochondria 10 0 2 9 3 27 62 54 33 71 97 82 1 1 0 2 4 3 1 1 0 0 0 1
Nucleus 1 0 0 0 0 0 1 0 0 0 0 2 0 0 2 0 4 1 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 3 3 0 0 0 0 0 0 0 7 1 3 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 9 9 1 0 0 0 0 1 0
Vac/Vac Membrane 1 1 0 3 0 0 4 2 0 3 1 2 0 0 0 10 12 5 0 7 0 0 0 2
Unique Cell Count 312 390 69 54 94 102 130 165 66 127 146 129 180 191 194 176 153 111 308 397 87 118 144 177
Labelled Cell Count 318 399 72 63 98 129 172 204 83 176 192 167 187 194 201 205 185 129 308 397 87 118 144 177


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 7.3 6.1 6.2 5.5 4.6 3.9 4.5 4.4 3.6 4.1 3.8 6.7 6.2 6.2 8.2 9.3 9.7 6.2 6.3 7.1
Std Deviation (1e-4) 0.6 1.0 1.3 2.1 1.7 0.9 1.3 2.1 2.2 0.7 1.7 1.2 0.9 1.2 1.2 1.5 1.7 2.4 1.5 1.5 1.2
Intensity Change (Log2) – – – 0.02 -0.17 -0.41 -0.65 -0.45 -0.49 -0.75 -0.59 -0.69 0.12 0.01 0.01 0.42 0.59 0.66 0.01 0.04 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.3 0.2 -0.9 -4.1 -3.1 -4.3 -4.5 -6.5 -6.1 1.6 0.8 1.7 -3.9 -3.6 -3.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 3.3 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.4048 4.2203 4.0131 3.7418 3.5164 3.7828 4.0807 4.3275 4.4279 4.2599 3.5415 4.2904 5.0475 5.8215 5.3925 4.7283 5.5245 5.2915
Actin 0.0102 0.0007 0.0012 0.0039 0.013 0.0019 0.0206 0.0029 0.02 0.0884 0.0605 0.0278 0.0675 0.0013 0.0131 0.0515 0.0042 0.0026
Bud 0.0012 0.0004 0.0022 0.0005 0.0013 0.0027 0.0007 0.0003 0.0029 0.0007 0.0004 0.0004 0.0015 0.0051 0.0059 0.0011 0.0003 0.0049
Bud Neck 0.0054 0.0002 0.0014 0.0004 0.001 0.0027 0.0128 0.0002 0.0012 0.0007 0.0006 0.0016 0.0218 0.0004 0.0028 0.0078 0.0005 0.0005
Bud Periphery 0.002 0.0003 0.0016 0.0004 0.0013 0.0032 0.0007 0.0002 0.0024 0.0014 0.0004 0.0006 0.0018 0.0025 0.0053 0.0008 0.0001 0.0021
Bud Site 0.0019 0.0012 0.0055 0.0003 0.0104 0.0013 0.0195 0.0031 0.0029 0.0039 0.0009 0.0007 0.0266 0.0156 0.0192 0.005 0.0015 0.0005
Cell Periphery 0.0008 0.0003 0.0005 0.0003 0.0002 0.0003 0.0005 0.0004 0.0003 0.0012 0.0002 0.0003 0.0007 0.0003 0.0006 0.0003 0.0001 0.0001
Cytoplasm 0.8084 0.9391 0.8854 0.8769 0.903 0.9005 0.6778 0.9367 0.8891 0.6952 0.8646 0.8581 0.6585 0.889 0.8162 0.7638 0.8563 0.9092
Cytoplasmic Foci 0.0166 0.0119 0.0169 0.0113 0.0195 0.0154 0.0301 0.0154 0.0219 0.0255 0.0097 0.0205 0.0445 0.0129 0.0228 0.0224 0.0361 0.0076
Eisosomes 0.0002 0 0.0001 0.0005 0.0001 0 0.0003 0 0 0.0069 0.0004 0.0004 0.0007 0 0.0003 0.001 0.0001 0
Endoplasmic Reticulum 0.017 0.0028 0.0064 0.0017 0.0048 0.0038 0.0134 0.0103 0.0031 0.0053 0.0032 0.0127 0.0129 0.0096 0.0049 0.0067 0.0026 0.0061
Endosome 0.0342 0.0097 0.0187 0.015 0.0258 0.0195 0.0598 0.011 0.0113 0.0375 0.0125 0.0155 0.0271 0.0076 0.0149 0.0293 0.0145 0.008
Golgi 0.0049 0.0011 0.0016 0.0005 0.0032 0.0039 0.0186 0.0028 0.007 0.0074 0.0226 0.0087 0.011 0.0015 0.0029 0.0069 0.002 0.0006
Lipid Particles 0.0023 0.0001 0.0004 0.0002 0.0007 0.002 0.0114 0.0004 0.0015 0.0054 0.0009 0.005 0.0176 0.0007 0.0023 0.0017 0.0003 0.0002
Mitochondria 0.0019 0.0007 0.0008 0.0004 0.0009 0.0015 0.0227 0.0003 0.0011 0.0156 0.0084 0.0049 0.0023 0.0002 0.0139 0.0042 0.0003 0.0005
None 0.0685 0.0257 0.0475 0.0762 0.0084 0.0266 0.0762 0.0104 0.0161 0.0912 0.0116 0.0282 0.0532 0.0482 0.0463 0.075 0.0745 0.0523
Nuclear Periphery 0.0041 0.0005 0.0011 0.0006 0.0007 0.0033 0.0054 0.0008 0.0004 0.0014 0.0005 0.0027 0.0037 0.0011 0.0019 0.0074 0.0007 0.0009
Nucleolus 0.0007 0 0.0002 0.0001 0.0001 0.0007 0.0008 0.0001 0 0.0008 0 0.0002 0.0012 0.0001 0.0012 0.0003 0.0001 0.0001
Nucleus 0.0057 0.001 0.0018 0.0011 0.001 0.0032 0.0031 0.0008 0.0005 0.0012 0.0005 0.0009 0.0022 0.0012 0.0024 0.0036 0.0007 0.0012
Peroxisomes 0.001 0.0003 0.0004 0.0006 0.0008 0.0023 0.0056 0.0003 0.0153 0.0057 0.0004 0.0074 0.0096 0.0003 0.008 0.0047 0.0016 0.0001
Punctate Nuclear 0.004 0.0004 0.0009 0.0002 0.0005 0.0006 0.009 0.0004 0.0004 0.0009 0.0002 0.0021 0.0317 0.0008 0.0106 0.0032 0.0011 0.0004
Vacuole 0.0079 0.0032 0.0049 0.0081 0.0026 0.0037 0.0098 0.0028 0.0023 0.003 0.0012 0.0011 0.0033 0.0015 0.004 0.0026 0.002 0.0016
Vacuole Periphery 0.001 0.0005 0.0007 0.0009 0.0006 0.0008 0.0014 0.0002 0.0004 0.0008 0.0004 0.0004 0.0006 0.0002 0.0007 0.0007 0.0003 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 26.0588 14.6426 18.7675 17.6255 23.1678 8.9295 15.9828 12.4905 10.3134 20.1875
Translational Efficiency 0.6027 1.0574 0.6547 0.5413 0.5825 1.2042 0.6864 0.5497 0.7725 0.6869

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein required for uridine thiolation of Lys(UUU) and Glu(UUC) tRNAs; required for the thiolation of uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae
Localization
Cell Percentages cytoplasm (94%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ncs2

Ncs2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ncs2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available