Standard name | |
---|---|
Human Ortholog | |
Description | Protein involved in recovery from cell cycle arrest; acts in response to pheromone; also involved in regulation of intra-S DNA damage checkpoint and autophagy; is essential for dephosphorylation of Atg13p; interacts with Far3p, Far7p, Far8p, Far9p, Far10p and with the phosphatases Pph21p, Pph22p and Pph3p; has similarity to the N- and C-termini of N. crassa HAM-2; similar to human Fam40A and Fam40B |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 8 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 13 | 11 | 6 | 9 | 5 | 7 | 3 | 12 | 10 |
Bud | 2 | 2 | 8 | 7 | 3 | 9 | 11 | 17 | 5 | 8 | 7 | 9 | 3 | 2 | 12 | 3 | 2 | 7 | 1 | 1 | 0 | 0 | 2 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 5 | 0 | 7 | 7 | 7 | 1 | 8 | 3 |
Bud Site | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 6 | 12 | 4 | 8 | 11 | 5 | 17 | 7 | 5 | 8 | 2 | 12 | 12 | 15 | 14 | 16 | 24 | 37 | 3 | 6 | 6 | 7 | 7 | 14 |
Cytoplasm | 94 | 124 | 116 | 171 | 211 | 200 | 237 | 343 | 118 | 144 | 122 | 164 | 156 | 231 | 267 | 241 | 167 | 219 | 54 | 86 | 39 | 53 | 90 | 95 |
Endoplasmic Reticulum | 63 | 35 | 6 | 1 | 1 | 1 | 7 | 4 | 2 | 5 | 5 | 6 | 2 | 6 | 1 | 35 | 16 | 18 | 106 | 66 | 31 | 14 | 30 | 85 |
Endosome | 1 | 1 | 0 | 2 | 2 | 0 | 6 | 8 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 7 | 6 | 6 | 2 | 1 | 0 | 0 | 2 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 4 | 2 | 1 | 0 | 0 | 0 |
Mitochondria | 1 | 5 | 22 | 28 | 26 | 75 | 126 | 244 | 98 | 153 | 126 | 216 | 3 | 3 | 4 | 10 | 6 | 5 | 18 | 19 | 57 | 16 | 21 | 21 |
Nucleus | 1 | 0 | 0 | 1 | 0 | 2 | 4 | 4 | 2 | 0 | 3 | 0 | 1 | 0 | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 |
Nuclear Periphery | 1 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 2 | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 3 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 2 | 1 | 1 | 1 | 5 | 3 | 11 | 20 | 5 | 14 | 4 | 12 | 0 | 1 | 3 | 11 | 16 | 8 | 6 | 8 | 5 | 5 | 8 | 10 |
Unique Cell Count | 163 | 170 | 137 | 192 | 232 | 245 | 342 | 537 | 182 | 261 | 214 | 329 | 164 | 242 | 278 | 288 | 223 | 267 | 217 | 206 | 161 | 109 | 189 | 251 |
Labelled Cell Count | 179 | 182 | 158 | 222 | 259 | 298 | 424 | 652 | 236 | 335 | 271 | 421 | 179 | 260 | 305 | 343 | 259 | 309 | 217 | 206 | 161 | 109 | 189 | 251 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.4 | 4.9 | 4.1 | 4.3 | 4.7 | 4.3 | 4.1 | 4.0 | 3.7 | 3.7 | 3.7 | 3.4 | 5.5 | 5.6 | 5.0 | 6.5 | 7.0 | 7.0 | 5.9 | 6.1 | 6.1 |
Std Deviation (1e-4) | 0.6 | 0.6 | 1.1 | 1.4 | 1.6 | 1.8 | 1.3 | 1.5 | 1.5 | 1.5 | 1.5 | 1.4 | 1.1 | 0.9 | 1.1 | 1.2 | 1.2 | 1.4 | 1.2 | 2.2 | 1.5 |
Intensity Change (Log2) | – | – | – | 0.06 | 0.2 | 0.06 | -0.0 | -0.03 | -0.17 | -0.15 | -0.14 | -0.26 | 0.44 | 0.45 | 0.29 | 0.68 | 0.78 | 0.78 | 0.52 | 0.57 | 0.58 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.8 | 0 | -0.7 | 0 | 0 | -1.6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.7 | 1.4 | 1.0 | 1.2 | 2.7 | 3.4 |
Cytoplasm | 1.2 | 1.8 | -0.8 | -3.5 | -4.7 | -4.0 | -5.9 | -5.4 | -7.0 | 3.1 | 3.6 | 4.1 | -0.3 | -2.2 | -0.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.5 | 1.1 | 1.0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -4.5 | -5.6 | -5.8 | -4.6 | -4.6 | -5.4 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 2.3 | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.0356 | 1.8531 | 2.0287 | 1.9027 | 2.4559 | 1.7196 | 2.0695 | 2.2248 | 2.2294 | 1.8189 | 1.5985 | 1.9976 | 1.8296 | 2.3356 | 1.9628 | 2.3389 | 2.0115 | 2.2469 |
Actin | 0.0417 | 0.0044 | 0.0336 | 0.0108 | 0.0354 | 0.0072 | 0.0292 | 0.0127 | 0.0225 | 0.0513 | 0.0363 | 0.0106 | 0.018 | 0.0012 | 0.0022 | 0.0136 | 0.0373 | 0.0055 |
Bud | 0.0026 | 0.0017 | 0.0032 | 0.0013 | 0.0006 | 0.0013 | 0.0011 | 0.0005 | 0.0026 | 0.0028 | 0.0005 | 0.0008 | 0.0004 | 0.0009 | 0.0005 | 0.0053 | 0.0002 | 0.0002 |
Bud Neck | 0.0065 | 0.0011 | 0.0076 | 0.005 | 0.0043 | 0.007 | 0.0067 | 0.0004 | 0.0019 | 0.0016 | 0.0012 | 0.0042 | 0.0007 | 0.0014 | 0.0023 | 0.0033 | 0.0008 | 0.0022 |
Bud Periphery | 0.0091 | 0.0018 | 0.0082 | 0.0036 | 0.0013 | 0.0035 | 0.0025 | 0.0011 | 0.0042 | 0.0072 | 0.001 | 0.0029 | 0.0012 | 0.0022 | 0.0011 | 0.01 | 0.0005 | 0.0006 |
Bud Site | 0.0072 | 0.0368 | 0.0259 | 0.0085 | 0.0017 | 0.0008 | 0.0131 | 0.0111 | 0.0105 | 0.0065 | 0.0017 | 0.0012 | 0.0028 | 0.0125 | 0.0042 | 0.0045 | 0.0003 | 0.0006 |
Cell Periphery | 0.0029 | 0.0031 | 0.0044 | 0.0013 | 0.0025 | 0.0022 | 0.0029 | 0.0024 | 0.0013 | 0.0007 | 0.0012 | 0.001 | 0.0033 | 0.004 | 0.0032 | 0.0022 | 0.0012 | 0.0013 |
Cytoplasm | 0.4682 | 0.5994 | 0.5936 | 0.4866 | 0.5418 | 0.6661 | 0.4228 | 0.5552 | 0.5336 | 0.5279 | 0.5085 | 0.5883 | 0.3555 | 0.558 | 0.5272 | 0.406 | 0.5599 | 0.5247 |
Cytoplasmic Foci | 0.0336 | 0.0182 | 0.0301 | 0.0155 | 0.0222 | 0.0259 | 0.0575 | 0.0106 | 0.032 | 0.0296 | 0.0303 | 0.0209 | 0.018 | 0.0122 | 0.0184 | 0.0128 | 0.0171 | 0.0251 |
Eisosomes | 0.0006 | 0.0002 | 0.0006 | 0.0004 | 0.0004 | 0.0002 | 0.0024 | 0.0004 | 0.0003 | 0.0005 | 0.0004 | 0.0002 | 0.0008 | 0.0003 | 0.0005 | 0.0005 | 0.0003 | 0.0002 |
Endoplasmic Reticulum | 0.0441 | 0.0257 | 0.0267 | 0.0328 | 0.0252 | 0.0355 | 0.0494 | 0.0231 | 0.0269 | 0.0281 | 0.0298 | 0.0251 | 0.0186 | 0.0125 | 0.024 | 0.0197 | 0.0107 | 0.0195 |
Endosome | 0.03 | 0.0116 | 0.0199 | 0.0188 | 0.052 | 0.0138 | 0.0407 | 0.0055 | 0.0252 | 0.0134 | 0.0581 | 0.0133 | 0.0084 | 0.0057 | 0.0134 | 0.0056 | 0.0029 | 0.006 |
Golgi | 0.0063 | 0.0029 | 0.0123 | 0.008 | 0.0193 | 0.0049 | 0.0156 | 0.004 | 0.0123 | 0.0072 | 0.0344 | 0.005 | 0.003 | 0.0006 | 0.0022 | 0.0009 | 0.0017 | 0.0018 |
Lipid Particles | 0.0121 | 0.0035 | 0.0147 | 0.0394 | 0.0288 | 0.0098 | 0.0219 | 0.0026 | 0.0094 | 0.003 | 0.0098 | 0.003 | 0.0078 | 0.001 | 0.0038 | 0.0012 | 0.0012 | 0.005 |
Mitochondria | 0.0147 | 0.001 | 0.014 | 0.0421 | 0.0153 | 0.0109 | 0.0095 | 0.0017 | 0.0055 | 0.0147 | 0.0249 | 0.0025 | 0.0027 | 0.0003 | 0.0014 | 0.0006 | 0.001 | 0.0021 |
None | 0.2421 | 0.2757 | 0.1798 | 0.2976 | 0.1934 | 0.1896 | 0.2395 | 0.3627 | 0.2816 | 0.2707 | 0.2468 | 0.3095 | 0.5203 | 0.3821 | 0.3867 | 0.504 | 0.3562 | 0.3866 |
Nuclear Periphery | 0.0335 | 0.002 | 0.0026 | 0.0089 | 0.0161 | 0.0081 | 0.0121 | 0.0008 | 0.006 | 0.0023 | 0.004 | 0.0038 | 0.0018 | 0.0007 | 0.0017 | 0.002 | 0.0005 | 0.0012 |
Nucleolus | 0.0009 | 0.0005 | 0.0008 | 0.0023 | 0.0019 | 0.0004 | 0.0026 | 0.0002 | 0.0003 | 0.0003 | 0.0002 | 0.0002 | 0.0011 | 0.0002 | 0.0003 | 0.0004 | 0.0001 | 0.0002 |
Nucleus | 0.0082 | 0.0023 | 0.0033 | 0.0022 | 0.0168 | 0.0051 | 0.0084 | 0.0009 | 0.0066 | 0.0015 | 0.0012 | 0.0013 | 0.0015 | 0.0011 | 0.0014 | 0.0019 | 0.0005 | 0.0011 |
Peroxisomes | 0.0052 | 0.0012 | 0.0092 | 0.0063 | 0.0043 | 0.0016 | 0.0284 | 0.0017 | 0.0115 | 0.0256 | 0.0036 | 0.0026 | 0.0289 | 0.0001 | 0.0006 | 0.0003 | 0.0021 | 0.0117 |
Punctate Nuclear | 0.0212 | 0.0023 | 0.004 | 0.0023 | 0.0101 | 0.0017 | 0.0272 | 0.0005 | 0.0028 | 0.0018 | 0.0017 | 0.0011 | 0.0028 | 0.0005 | 0.0011 | 0.0022 | 0.0046 | 0.0025 |
Vacuole | 0.0058 | 0.004 | 0.0049 | 0.0041 | 0.0042 | 0.0032 | 0.005 | 0.0018 | 0.0024 | 0.0027 | 0.0029 | 0.0019 | 0.0022 | 0.0023 | 0.0033 | 0.0026 | 0.0009 | 0.0014 |
Vacuole Periphery | 0.0035 | 0.0004 | 0.0006 | 0.0024 | 0.0025 | 0.0012 | 0.0015 | 0.0001 | 0.0008 | 0.0006 | 0.0013 | 0.0006 | 0.0002 | 0.0001 | 0.0003 | 0.0003 | 0.0001 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 18.5057 | 6.5483 | 8.951 | 13.214 | 10.4972 | 11.1744 | 10.9768 | 13.1709 | 12.277 | 14.8603 |
Translational Efficiency | 0.478 | 0.7478 | 0.8577 | 0.6859 | 0.69 | 0.9243 | 0.5946 | 0.4989 | 0.6232 | 0.5577 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
493 | 1223 | 1765 | 149 | 1400 | 1913 | 196 | 75 | 1893 | 3136 | 1961 | 224 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 703.57 | 830.02 | 1068.77 | 924.48 | 632.67 | 813.45 | 977.18 | 940.13 | 651.13 | 819.91 | 1059.62 | 929.72 |
Standard Deviation | 76.94 | 109.33 | 123.72 | 114.27 | 92.51 | 100.95 | 101.37 | 122.57 | 94.01 | 104.61 | 124.73 | 117.34 |
Intensity Change Log 2 | — | 0.238452 | 0.603186 | 0.393948 | — | 0.362600 | 0.627171 | 0.571407 | — | 0.298566 | 0.614592 | 0.480695 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001227 | 0.002781 | 0.000447 | 0.000669 | 0.001019 | 0.001309 | 0.000271 | 0.000618 | 0.001073 | 0.001883 | 0.000430 | 0.000652 |
Bud Neck | 0.041117 | 0.024721 | 0.002931 | 0.007906 | 0.016753 | 0.038186 | 0.004412 | 0.004208 | 0.023098 | 0.032935 | 0.003079 | 0.006668 |
Bud Site | 0.022859 | 0.029293 | 0.011106 | 0.061977 | 0.008227 | 0.028469 | 0.003748 | 0.013566 | 0.012037 | 0.028791 | 0.010370 | 0.045768 |
Cell Periphery | 0.002076 | 0.000640 | 0.000285 | 0.000213 | 0.000321 | 0.000701 | 0.000405 | 0.000536 | 0.000778 | 0.000677 | 0.000297 | 0.000321 |
Cytoplasm | 0.356693* | 0.314519* | 0.647024* | 0.363464* | 0.317110* | 0.322003* | 0.347285* | 0.342522* | 0.327419* | 0.319084* | 0.617065* | 0.356452* |
Cytoplasmic Foci | 0.235334* | 0.338037* | 0.047930 | 0.068738 | 0.326975* | 0.279657* | 0.079128 | 0.127346 | 0.303109* | 0.302424* | 0.051048 | 0.088361 |
Eisosomes | 0.002347 | 0.001208 | 0.000042 | 0.000119 | 0.000597 | 0.001273 | 0.000197 | 0.000258 | 0.001053 | 0.001247 | 0.000058 | 0.000166 |
Endoplasmic Reticulum | 0.040033 | 0.026584 | 0.006876 | 0.002178 | 0.024106 | 0.022866 | 0.005534 | 0.009574 | 0.028254 | 0.024316 | 0.006742 | 0.004654 |
Endosome | 0.046503 | 0.043366 | 0.002987 | 0.015324 | 0.044131 | 0.054046 | 0.011824 | 0.010335 | 0.044749 | 0.049881 | 0.003870 | 0.013653 |
Golgi | 0.026778 | 0.026010 | 0.000217 | 0.002349 | 0.018952 | 0.027555 | 0.000125 | 0.000457 | 0.020990 | 0.026953 | 0.000208 | 0.001715 |
Lipid Particles | 0.076707 | 0.031801 | 0.002457 | 0.003509 | 0.069255 | 0.036928 | 0.007194 | 0.007378 | 0.071196 | 0.034928 | 0.002930 | 0.004804 |
Mitochondria | 0.016681 | 0.011181 | 0.000172 | 0.000401 | 0.015368 | 0.017457 | 0.000134 | 0.021215 | 0.015710 | 0.015010 | 0.000168 | 0.007370 |
Mitotic Spindle | 0.005795 | 0.001855 | 0.001566 | 0.018643 | 0.000523 | 0.002830 | 0.000164 | 0.013465 | 0.001896 | 0.002450 | 0.001426 | 0.016910 |
None | 0.000794 | 0.000548 | 0.001909 | 0.000943 | 0.006541 | 0.000639 | 0.001966 | 0.000843 | 0.005044 | 0.000604 | 0.001914 | 0.000910 |
Nuclear Periphery | 0.001304 | 0.000555 | 0.000785 | 0.001397 | 0.004101 | 0.000435 | 0.001974 | 0.007469 | 0.003373 | 0.000482 | 0.000904 | 0.003430 |
Nuclear Periphery Foci | 0.001096 | 0.001673 | 0.012599 | 0.005360 | 0.002797 | 0.000861 | 0.007716 | 0.017957 | 0.002354 | 0.001178 | 0.012111 | 0.009578 |
Nucleolus | 0.000432 | 0.000418 | 0.000138 | 0.000262 | 0.002997 | 0.000891 | 0.000533 | 0.000377 | 0.002329 | 0.000706 | 0.000177 | 0.000300 |
Nucleus | 0.033795 | 0.034176 | 0.176533* | 0.048415 | 0.058762 | 0.033460 | 0.198549* | 0.067956 | 0.052260 | 0.033739 | 0.178734* | 0.054958 |
Peroxisomes | 0.010842 | 0.012957 | 0.000283 | 0.001707 | 0.007925 | 0.011082 | 0.000640 | 0.003663 | 0.008685 | 0.011813 | 0.000319 | 0.002362 |
Vacuole | 0.072630 | 0.092428 | 0.083510 | 0.395727* | 0.067868 | 0.113759 | 0.328016* | 0.349326* | 0.069108 | 0.105440 | 0.107948 | 0.380191* |
Vacuole Periphery | 0.004955 | 0.005252 | 0.000204 | 0.000700 | 0.005671 | 0.005592 | 0.000184 | 0.000930 | 0.005485 | 0.005460 | 0.000202 | 0.000777 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.19 | 2.33 | 2.03 | 5.74 | -0.61 | -0.74 | 1.83 | 0.84 | 3.65 | -2.89 | -2.44 | 2.03 | 1.56 | 7.05 | -1.48 |
Bud Neck | 4.09 | 10.32 | 9.16 | 9.02 | -1.50 | -9.93 | 9.41 | 9.28 | 18.01 | 0.18 | -5.34 | 14.63 | 11.23 | 17.83 | -1.52 |
Bud Site | -1.87 | 3.75 | -0.71 | -0.07 | -1.97 | -10.91 | 5.27 | -0.88 | 3.59 | -1.85 | -10.73 | 1.12 | -1.82 | 0.74 | -2.05 |
Cell Periphery | 6.18 | 7.80 | 8.32 | 8.11 | 4.44 | -8.93 | -1.23 | -1.99 | 2.75 | -1.41 | 1.46 | 7.26 | 7.21 | 9.23 | 0.90 |
Cytoplasm | 3.41 | -22.70 | 2.52 | 0.94 | 13.60 | -0.65 | 1.15 | 0.90 | 1.08 | 0.21 | 1.15 | -33.87 | 1.87 | 1.50 | 15.11 |
Cytoplasmic Foci | -10.81 | 24.53 | 19.85 | 35.17 | -0.19 | 6.86 | 33.35 | 15.00 | 12.09 | -2.44 | 0.18 | 52.93 | 30.73 | 32.71 | -2.58 |
Eisosomes | 4.21 | 11.24 | 10.88 | 6.07 | -4.78 | -6.82 | 8.25 | 6.46 | 11.31 | -1.23 | -1.75 | 14.95 | 13.25 | 12.30 | -6.39 |
Endoplasmic Reticulum | 2.44 | 6.49 | 7.42 | 10.94 | 9.47 | 0.42 | 8.16 | 4.85 | 4.72 | -0.76 | 1.59 | 10.33 | 10.83 | 12.14 | 2.73 |
Endosome | 0.76 | 11.47 | 8.97 | 12.09 | -1.95 | -3.67 | 14.98 | 15.04 | 20.96 | 1.23 | -2.25 | 22.88 | 15.87 | 20.85 | -2.64 |
Golgi | 0.33 | 12.31 | 11.53 | 15.62 | -0.89 | -5.66 | 18.66 | 18.28 | 23.59 | -2.00 | -4.57 | 22.17 | 19.66 | 26.16 | -1.02 |
Lipid Particles | 7.70 | 13.18 | 13.15 | 17.84 | -0.05 | 9.57 | 20.35 | 18.97 | 14.80 | 0.05 | 12.55 | 25.55 | 24.73 | 24.85 | -1.69 |
Mitochondria | 2.37 | 8.18 | 8.16 | 9.17 | -0.32 | -1.09 | 10.28 | 0.19 | 0.36 | -1.01 | 0.46 | 12.80 | 2.56 | 2.45 | -1.01 |
Mitotic Spindle | 1.40 | 1.33 | -1.37 | -2.07 | -2.04 | -3.66 | 2.60 | -1.64 | -1.25 | -1.69 | -0.65 | 0.03 | -2.45 | -2.35 | -2.46 |
None | 1.13 | -2.93 | -2.35 | -2.83 | -0.67 | 5.96 | 4.83 | 5.39 | -0.91 | 1.07 | 6.03 | 4.30 | 3.98 | -2.92 | -0.08 |
Nuclear Periphery | 1.72 | 1.02 | 0.10 | -2.33 | -1.35 | 4.91 | 2.51 | -0.22 | -1.12 | -0.71 | 5.12 | 4.20 | 0.57 | -1.47 | -1.10 |
Nuclear Periphery Foci | -2.27 | -16.84 | -2.88 | -2.27 | 7.12 | 7.86 | -4.17 | -1.75 | -2.07 | -0.97 | 5.79 | -15.23 | -2.25 | -2.79 | 2.33 |
Nucleolus | 0.15 | 9.19 | 3.84 | 3.52 | -1.60 | 5.11 | 6.55 | 5.86 | 1.79 | 0.19 | 5.40 | 7.50 | 6.90 | 3.35 | -1.22 |
Nucleus | -0.19 | -34.43 | -3.83 | -3.82 | 12.38 | 9.37 | -9.06 | -1.21 | -2.84 | 5.33 | 8.66 | -31.65 | -2.29 | -4.78 | 12.97 |
Peroxisomes | -1.69 | 12.46 | 10.98 | 12.51 | -2.62 | -4.54 | 15.38 | 1.89 | 3.22 | -1.28 | -4.96 | 20.09 | 7.63 | 10.79 | -1.96 |
Vacuole | -3.54 | -5.34 | -16.13 | -15.49 | -15.11 | -11.07 | -15.95 | -9.98 | -8.74 | -0.96 | -11.18 | -13.57 | -19.08 | -17.40 | -15.97 |
Vacuole Periphery | -0.47 | 11.11 | 10.60 | 11.58 | -0.76 | 0.11 | 12.32 | 7.85 | 9.25 | -0.92 | 0.04 | 15.13 | 13.12 | 17.55 | -1.13 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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