Standard name
Human Ortholog
Description Subunit of the NuA4 histone acetyltransferase complex; NuA4 acetylates the N-terminal tails of histones H4 and H2A

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0.05 0 0 0 0 0.05 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.07 0 0 0 0 0 0 0.06 0.07 0.13 0.16 0.1 0.09 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.35 0.1 0.11 0.21 0.2 0.34 0.47 0.39 0.56 0.61 0.59 0.57 0 0 0 0.07 0.05 0.06 0 0 0 0 0 0
Nucleus 0.92 0.95 0.88 0.95 0.82 0.85 0.74 0.74 0.74 0.72 0.74 0.75 0.91 0.85 0.81 0.85 0.86 0.86 0.81 0.85 0.79 0.82 0.76 0.75
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.16 0.11 0.17 0.21 0.1 0.2 0.17 0.19 0.15 0.17 0.23 0.25 0 0 0 0 0 0 0.09 0.09 0.12 0.08 0.1 0.11
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.05 0.06 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1 1 0 0 3 1 1 2 2 2 0 0 0 0 0 1 2 0 1 2 6 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2
Bud Site 5 0 0 0 0 3 12 6 8 6 3 6 0 0 1 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 3 1 4 1 9 3 6 7 6 7 3 7 33 76 114 16 14 10 0 0 0 1 5 3
Endoplasmic Reticulum 0 1 0 0 0 0 0 0 0 0 0 0 1 1 1 3 4 2 0 0 0 0 0 0
Endosome 0 0 1 0 0 0 0 0 0 0 0 0 1 1 3 2 0 1 4 2 0 0 1 7
Golgi 0 0 0 0 0 0 1 0 0 0 0 0 1 1 1 0 0 0 1 1 0 0 2 1
Mitochondria 85 24 14 8 27 57 113 69 114 141 90 72 1 1 0 11 8 8 1 0 0 0 6 8
Nucleus 224 223 113 36 112 143 177 129 150 166 112 94 410 488 566 139 130 113 204 204 110 244 385 390
Nuclear Periphery 1 0 0 0 1 1 1 0 2 1 0 2 1 2 0 0 3 1 0 0 0 0 1 2
Nucleolus 40 25 22 8 14 33 42 34 30 39 35 31 0 2 1 3 2 1 22 22 17 24 52 57
Peroxisomes 0 0 0 0 1 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0 0 0
SpindlePole 2 1 3 0 0 0 1 1 0 0 1 0 14 14 15 0 1 1 6 2 3 7 10 10
Vac/Vac Membrane 0 1 1 0 1 0 6 0 4 1 3 0 1 0 7 4 7 4 7 5 4 14 29 31
Unique Cell Count 244 234 128 38 136 169 240 175 203 232 152 126 451 571 696 164 152 131 253 241 140 299 507 521
Labelled Cell Count 360 276 159 54 165 240 362 247 315 363 249 214 463 587 711 179 169 142 253 241 140 299 507 521


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 5.8 5.5 4.8 5.2 4.5 4.6 4.3 4.1 4.0 3.8 3.8 6.8 6.7 6.5 7.6 7.3 7.7 6.5 6.8 7.0
Std Deviation (1e-4) 0.6 1.0 1.4 1.0 1.8 1.2 2.0 1.5 1.3 1.1 2.2 1.3 1.1 1.2 1.2 1.5 1.9 1.7 1.2 1.1 1.3
Intensity Change (Log2) -0.2 -0.08 -0.28 -0.27 -0.37 -0.45 -0.47 -0.52 -0.53 0.29 0.29 0.24 0.45 0.4 0.48 0.24 0.29 0.35


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 1.3 0 0 0 0 0 0 1.0 1.7 3.3 3.9 2.2 2.1 1.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 2.0 0 0 0 0 0 0 0 0 0 0 -1.3 -1.8 -1.4
Nucleus 1.2 -1.4 -0.9 -3.2 -3.1 -3.2 -3.6 -3.1 -2.8 0.9 -0.8 -1.9 -0.9 -0.7 -0.5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 -1.6 0 0 0 0 0 0 0 0 0 0 -4.7 -4.7 -4.6
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.8152 2.4505 2.266 1.928 1.7981 2.1353 1.8155 0.9478 1.0898 0.6013 0.4519 0.6373 1.2119 1.1877 0.6442 0.8844 0.5241 0.5026
Actin 0.0141 0.0091 0.0122 0.0001 0.0303 0 0.0587 0.0017 0.0018 0.0009 0.0024 0.0009 0.0223 0 0.0002 0.0063 0.0002 0.0032
Bud 0.0009 0.0001 0.0001 0 0.0003 0 0.0013 0 0.0005 0.0001 0.0001 0.0001 0.0009 0 0.0001 0.0016 0.0004 0.0001
Bud Neck 0.0082 0.0003 0.0012 0.0002 0.0022 0.0006 0.0045 0.0001 0.0009 0.0002 0.0005 0.0013 0.005 0.0001 0.0003 0.0004 0.0006 0.0011
Bud Periphery 0.0016 0.0001 0.0001 0 0.0006 0 0.0029 0 0.0012 0.0002 0.0001 0.0004 0.0022 0 0.0001 0.002 0.0003 0.0002
Bud Site 0.0049 0.0008 0.0036 0.0001 0.0008 0 0.0038 0.0001 0.0081 0.0003 0.0002 0.0003 0.0032 0.0001 0.0009 0.001 0.0004 0.0002
Cell Periphery 0.0004 0.0002 0.0001 0 0.0002 0 0.0003 0 0.0002 0 0 0.0001 0.0006 0 0.0001 0.0002 0.0001 0
Cytoplasm 0.0124 0.005 0.0003 0.001 0.0017 0.0001 0.008 0.0005 0.0035 0.0008 0.0018 0.0006 0.0037 0.0002 0.0011 0.004 0.0106 0.0001
Cytoplasmic Foci 0.0014 0.0006 0.0103 0.0003 0.0053 0 0.0267 0.0003 0.0058 0.001 0.0021 0.0036 0.0224 0.0001 0.0003 0.0074 0.0356 0.0011
Eisosomes 0.0005 0.0001 0.0001 0.0001 0.0002 0 0.0004 0 0 0 0 0 0.0003 0 0 0.0002 0 0
Endoplasmic Reticulum 0.0027 0.008 0.0001 0.0001 0.0018 0 0.0058 0.0006 0.0015 0.001 0.003 0.0001 0.002 0 0.0003 0.0009 0.0006 0.0002
Endosome 0.006 0.0006 0.0018 0.0001 0.006 0 0.0362 0.0016 0.0082 0.0033 0.0198 0.0045 0.0262 0 0.0084 0.0155 0.0551 0.0023
Golgi 0.0007 0.0009 0.001 0 0.0042 0 0.0138 0.0005 0.0029 0.0006 0.0016 0.003 0.01 0 0.0008 0.011 0.0041 0.0038
Lipid Particles 0.0005 0.0002 0.0043 0.0002 0.0067 0 0.0284 0.0003 0.0019 0.0034 0.0022 0.006 0.0271 0 0.0018 0.0197 0.0016 0.0025
Mitochondria 0.0014 0.0002 0.0003 0.0001 0.0025 0.0001 0.0084 0.0005 0.0067 0.0019 0.0008 0.0045 0.0119 0.0001 0.0009 0.0164 0.003 0.0034
None 0.0106 0.0004 0.0003 0.0462 0.0026 0 0.0074 0.0002 0.0057 0.0004 0.0005 0.0002 0.0034 0 0.0004 0.0012 0.0054 0
Nuclear Periphery 0.0105 0.0017 0.0025 0.001 0.0132 0.0002 0.0346 0.0039 0.0095 0.015 0.017 0.0022 0.0088 0.0005 0.0056 0.0072 0.0039 0.005
Nucleolus 0.0132 0.0155 0.0147 0.0071 0.0458 0.0416 0.0229 0.0164 0.0121 0.0131 0.0671 0.0409 0.0254 0.0175 0.0139 0.0094 0.1112 0.0434
Nucleus 0.8943 0.9553 0.9363 0.9412 0.8491 0.9556 0.6658 0.967 0.8999 0.9442 0.8731 0.9237 0.774 0.9666 0.9543 0.8554 0.7044 0.9189
Peroxisomes 0.0003 0 0.0038 0 0.0043 0 0.0206 0.0002 0.0044 0.0006 0.0003 0.0025 0.0104 0 0.0001 0.0025 0.0002 0.0011
Punctate Nuclear 0.0101 0.0008 0.0064 0.0021 0.0205 0.0016 0.0445 0.0057 0.0205 0.0115 0.0063 0.0039 0.032 0.0147 0.0087 0.0338 0.056 0.0128
Vacuole 0.0045 0.0001 0.0005 0.0001 0.0013 0 0.0031 0.0001 0.0032 0.0007 0.0005 0.0006 0.006 0 0.0012 0.0017 0.0042 0.0002
Vacuole Periphery 0.0009 0.0001 0.0001 0 0.0006 0 0.0022 0.0001 0.0014 0.0007 0.0005 0.0005 0.0022 0 0.0005 0.0022 0.0022 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3.0218 10.0336 5.1545 8.7651 10.6344 6.9032 8.7793 11.0608 13.0594 8.6295
Translational Efficiency 4.6471 0.9493 1.8079 1.5656 0.8812 1.4192 0.9738 0.8955 0.8074 0.9401

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Subunit of the NuA4 histone acetyltransferase complex; NuA4 acetylates the N-terminal tails of histones H4 and H2A
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Eaf7

Eaf7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Eaf7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available