Standard name | |
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Human Ortholog | |
Description | Subunit of the NuA4 histone acetyltransferase complex; NuA4 acetylates the N-terminal tails of histones H4 and H2A |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 1 | 1 | 0 | 0 | 3 | 1 | 1 | 2 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 2 | 6 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 |
Bud Site | 5 | 0 | 0 | 0 | 0 | 3 | 12 | 6 | 8 | 6 | 3 | 6 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 1 | 4 | 1 | 9 | 3 | 6 | 7 | 6 | 7 | 3 | 7 | 33 | 76 | 114 | 16 | 14 | 10 | 0 | 0 | 0 | 1 | 5 | 3 |
Endoplasmic Reticulum | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 3 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 2 | 0 | 1 | 4 | 2 | 0 | 0 | 1 | 7 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 1 |
Mitochondria | 85 | 24 | 14 | 8 | 27 | 57 | 113 | 69 | 114 | 141 | 90 | 72 | 1 | 1 | 0 | 11 | 8 | 8 | 1 | 0 | 0 | 0 | 6 | 8 |
Nucleus | 224 | 223 | 113 | 36 | 112 | 143 | 177 | 129 | 150 | 166 | 112 | 94 | 410 | 488 | 566 | 139 | 130 | 113 | 204 | 204 | 110 | 244 | 385 | 390 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 2 | 1 | 0 | 2 | 1 | 2 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 1 | 2 |
Nucleolus | 40 | 25 | 22 | 8 | 14 | 33 | 42 | 34 | 30 | 39 | 35 | 31 | 0 | 2 | 1 | 3 | 2 | 1 | 22 | 22 | 17 | 24 | 52 | 57 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 2 | 1 | 3 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 14 | 14 | 15 | 0 | 1 | 1 | 6 | 2 | 3 | 7 | 10 | 10 |
Vac/Vac Membrane | 0 | 1 | 1 | 0 | 1 | 0 | 6 | 0 | 4 | 1 | 3 | 0 | 1 | 0 | 7 | 4 | 7 | 4 | 7 | 5 | 4 | 14 | 29 | 31 |
Unique Cell Count | 244 | 234 | 128 | 38 | 136 | 169 | 240 | 175 | 203 | 232 | 152 | 126 | 451 | 571 | 696 | 164 | 152 | 131 | 253 | 241 | 140 | 299 | 507 | 521 |
Labelled Cell Count | 360 | 276 | 159 | 54 | 165 | 240 | 362 | 247 | 315 | 363 | 249 | 214 | 463 | 587 | 711 | 179 | 169 | 142 | 253 | 241 | 140 | 299 | 507 | 521 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.4 | 5.8 | 5.5 | 4.8 | 5.2 | 4.5 | 4.6 | 4.3 | 4.1 | 4.0 | 3.8 | 3.8 | 6.8 | 6.7 | 6.5 | 7.6 | 7.3 | 7.7 | 6.5 | 6.8 | 7.0 |
Std Deviation (1e-4) | 0.6 | 1.0 | 1.4 | 1.0 | 1.8 | 1.2 | 2.0 | 1.5 | 1.3 | 1.1 | 2.2 | 1.3 | 1.1 | 1.2 | 1.2 | 1.5 | 1.9 | 1.7 | 1.2 | 1.1 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.2 | -0.08 | -0.28 | -0.27 | -0.37 | -0.45 | -0.47 | -0.52 | -0.53 | 0.29 | 0.29 | 0.24 | 0.45 | 0.4 | 0.48 | 0.24 | 0.29 | 0.35 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 1.3 | 0 | 0 | 0 | 0 | 0 | 0 | 1.0 | 1.7 | 3.3 | 3.9 | 2.2 | 2.1 | 1.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 2.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.3 | -1.8 | -1.4 |
Nucleus | 1.2 | -1.4 | -0.9 | -3.2 | -3.1 | -3.2 | -3.6 | -3.1 | -2.8 | 0.9 | -0.8 | -1.9 | -0.9 | -0.7 | -0.5 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | -1.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -4.7 | -4.7 | -4.6 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.8152 | 2.4505 | 2.266 | 1.928 | 1.7981 | 2.1353 | 1.8155 | 0.9478 | 1.0898 | 0.6013 | 0.4519 | 0.6373 | 1.2119 | 1.1877 | 0.6442 | 0.8844 | 0.5241 | 0.5026 |
Actin | 0.0141 | 0.0091 | 0.0122 | 0.0001 | 0.0303 | 0 | 0.0587 | 0.0017 | 0.0018 | 0.0009 | 0.0024 | 0.0009 | 0.0223 | 0 | 0.0002 | 0.0063 | 0.0002 | 0.0032 |
Bud | 0.0009 | 0.0001 | 0.0001 | 0 | 0.0003 | 0 | 0.0013 | 0 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0009 | 0 | 0.0001 | 0.0016 | 0.0004 | 0.0001 |
Bud Neck | 0.0082 | 0.0003 | 0.0012 | 0.0002 | 0.0022 | 0.0006 | 0.0045 | 0.0001 | 0.0009 | 0.0002 | 0.0005 | 0.0013 | 0.005 | 0.0001 | 0.0003 | 0.0004 | 0.0006 | 0.0011 |
Bud Periphery | 0.0016 | 0.0001 | 0.0001 | 0 | 0.0006 | 0 | 0.0029 | 0 | 0.0012 | 0.0002 | 0.0001 | 0.0004 | 0.0022 | 0 | 0.0001 | 0.002 | 0.0003 | 0.0002 |
Bud Site | 0.0049 | 0.0008 | 0.0036 | 0.0001 | 0.0008 | 0 | 0.0038 | 0.0001 | 0.0081 | 0.0003 | 0.0002 | 0.0003 | 0.0032 | 0.0001 | 0.0009 | 0.001 | 0.0004 | 0.0002 |
Cell Periphery | 0.0004 | 0.0002 | 0.0001 | 0 | 0.0002 | 0 | 0.0003 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0.0006 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 |
Cytoplasm | 0.0124 | 0.005 | 0.0003 | 0.001 | 0.0017 | 0.0001 | 0.008 | 0.0005 | 0.0035 | 0.0008 | 0.0018 | 0.0006 | 0.0037 | 0.0002 | 0.0011 | 0.004 | 0.0106 | 0.0001 |
Cytoplasmic Foci | 0.0014 | 0.0006 | 0.0103 | 0.0003 | 0.0053 | 0 | 0.0267 | 0.0003 | 0.0058 | 0.001 | 0.0021 | 0.0036 | 0.0224 | 0.0001 | 0.0003 | 0.0074 | 0.0356 | 0.0011 |
Eisosomes | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0 | 0.0002 | 0 | 0 |
Endoplasmic Reticulum | 0.0027 | 0.008 | 0.0001 | 0.0001 | 0.0018 | 0 | 0.0058 | 0.0006 | 0.0015 | 0.001 | 0.003 | 0.0001 | 0.002 | 0 | 0.0003 | 0.0009 | 0.0006 | 0.0002 |
Endosome | 0.006 | 0.0006 | 0.0018 | 0.0001 | 0.006 | 0 | 0.0362 | 0.0016 | 0.0082 | 0.0033 | 0.0198 | 0.0045 | 0.0262 | 0 | 0.0084 | 0.0155 | 0.0551 | 0.0023 |
Golgi | 0.0007 | 0.0009 | 0.001 | 0 | 0.0042 | 0 | 0.0138 | 0.0005 | 0.0029 | 0.0006 | 0.0016 | 0.003 | 0.01 | 0 | 0.0008 | 0.011 | 0.0041 | 0.0038 |
Lipid Particles | 0.0005 | 0.0002 | 0.0043 | 0.0002 | 0.0067 | 0 | 0.0284 | 0.0003 | 0.0019 | 0.0034 | 0.0022 | 0.006 | 0.0271 | 0 | 0.0018 | 0.0197 | 0.0016 | 0.0025 |
Mitochondria | 0.0014 | 0.0002 | 0.0003 | 0.0001 | 0.0025 | 0.0001 | 0.0084 | 0.0005 | 0.0067 | 0.0019 | 0.0008 | 0.0045 | 0.0119 | 0.0001 | 0.0009 | 0.0164 | 0.003 | 0.0034 |
None | 0.0106 | 0.0004 | 0.0003 | 0.0462 | 0.0026 | 0 | 0.0074 | 0.0002 | 0.0057 | 0.0004 | 0.0005 | 0.0002 | 0.0034 | 0 | 0.0004 | 0.0012 | 0.0054 | 0 |
Nuclear Periphery | 0.0105 | 0.0017 | 0.0025 | 0.001 | 0.0132 | 0.0002 | 0.0346 | 0.0039 | 0.0095 | 0.015 | 0.017 | 0.0022 | 0.0088 | 0.0005 | 0.0056 | 0.0072 | 0.0039 | 0.005 |
Nucleolus | 0.0132 | 0.0155 | 0.0147 | 0.0071 | 0.0458 | 0.0416 | 0.0229 | 0.0164 | 0.0121 | 0.0131 | 0.0671 | 0.0409 | 0.0254 | 0.0175 | 0.0139 | 0.0094 | 0.1112 | 0.0434 |
Nucleus | 0.8943 | 0.9553 | 0.9363 | 0.9412 | 0.8491 | 0.9556 | 0.6658 | 0.967 | 0.8999 | 0.9442 | 0.8731 | 0.9237 | 0.774 | 0.9666 | 0.9543 | 0.8554 | 0.7044 | 0.9189 |
Peroxisomes | 0.0003 | 0 | 0.0038 | 0 | 0.0043 | 0 | 0.0206 | 0.0002 | 0.0044 | 0.0006 | 0.0003 | 0.0025 | 0.0104 | 0 | 0.0001 | 0.0025 | 0.0002 | 0.0011 |
Punctate Nuclear | 0.0101 | 0.0008 | 0.0064 | 0.0021 | 0.0205 | 0.0016 | 0.0445 | 0.0057 | 0.0205 | 0.0115 | 0.0063 | 0.0039 | 0.032 | 0.0147 | 0.0087 | 0.0338 | 0.056 | 0.0128 |
Vacuole | 0.0045 | 0.0001 | 0.0005 | 0.0001 | 0.0013 | 0 | 0.0031 | 0.0001 | 0.0032 | 0.0007 | 0.0005 | 0.0006 | 0.006 | 0 | 0.0012 | 0.0017 | 0.0042 | 0.0002 |
Vacuole Periphery | 0.0009 | 0.0001 | 0.0001 | 0 | 0.0006 | 0 | 0.0022 | 0.0001 | 0.0014 | 0.0007 | 0.0005 | 0.0005 | 0.0022 | 0 | 0.0005 | 0.0022 | 0.0022 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 3.0218 | 10.0336 | 5.1545 | 8.7651 | 10.6344 | 6.9032 | 8.7793 | 11.0608 | 13.0594 | 8.6295 |
Translational Efficiency | 4.6471 | 0.9493 | 1.8079 | 1.5656 | 0.8812 | 1.4192 | 0.9738 | 0.8955 | 0.8074 | 0.9401 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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