Standard name
Human Ortholog
Description Adenine deaminase (adenine aminohydrolase); converts adenine to hypoxanthine; involved in purine salvage; transcriptionally regulated by nutrient levels and growth phase; Aah1p degraded upon entry into quiescence via SCF and the proteasome
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 1.0 0.97 0.93 0.92 0.9 0.79 0.77 0.76 0.72 0.63 0.68 0.99 0.99 1.0 0.97 0.97 0.99 0.95 0.96 0.94 0.96 0.93 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.15 0.11 0.3 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.09 0.08 0.08 0.16 0.15 0.08 0.17 0.12 0.2 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 3 2 1 0 3 0 0 0 0 0 0 1 0 1 0 0 0
Bud 0 0 0 0 0 0 2 3 8 5 10 4 0 0 0 1 0 0 3 0 0 0 7 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 2 3 5 2 5 10 8 2 4 13 1 1 0 3 5 4 1 0 0 0 1 0
Cytoplasm 343 83 170 256 390 316 401 314 279 231 166 224 255 236 254 515 303 385 324 93 162 110 315 261
Endoplasmic Reticulum 0 0 0 1 8 7 12 10 2 5 4 9 2 2 0 12 10 14 1 0 0 0 4 2
Endosome 0 0 0 1 0 1 1 2 2 1 1 0 0 0 0 1 0 0 1 0 0 0 0 0
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Mitochondria 3 0 0 0 0 5 18 14 56 35 79 36 0 0 0 1 1 0 0 0 0 0 0 0
Nucleus 1 0 6 3 2 6 8 9 14 10 9 9 0 1 1 1 1 0 2 1 2 0 2 2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane 2 0 2 25 36 28 79 61 31 56 32 66 2 2 2 18 6 6 1 0 1 0 3 1
Unique Cell Count 345 83 175 276 424 353 509 408 369 320 262 331 257 238 254 531 311 390 342 98 173 115 339 278
Labelled Cell Count 350 84 180 291 441 365 526 426 402 347 305 364 260 242 257 552 326 410 342 98 173 115 339 278


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 17.5 21.4 19.0 18.9 13.8 9.6 8.4 8.6 7.3 7.7 6.9 7.7 22.2 21.0 18.5 34.7 38.5 38.4 25.2 26.1 23.4
Std Deviation (1e-4) 4.8 5.3 4.8 4.9 2.9 2.2 2.3 2.2 2.0 2.3 2.0 2.1 6.0 5.7 5.3 8.4 10.4 9.8 8.8 8.4 6.7
Intensity Change (Log2) – – – -0.01 -0.46 -0.98 -1.17 -1.14 -1.38 -1.3 -1.45 -1.29 0.23 0.14 -0.03 0.87 1.02 1.02 0.41 0.46 0.31


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 1.8 0 0 0 0 0 0
Cytoplasm -2.0 -2.3 -3.0 -5.6 -5.9 -6.2 -6.8 -8.2 -7.6 1.7 1.6 2.7 -0.1 0.2 1.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 5.4 4.5 8.0 4.5 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0.2 -0.2 0 -0.4 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 3.5 3.4 3.2 5.1 4.9 3.3 5.4 4.2 5.9 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 13.6789 17.6315 16.1908 14.36 13.0271 15.9184 12.4758 16.1214 15.7435 14.8556 11.647 15.6617 13.0754 17.9726 18.17 18.9294 15.6896 17.1189
Actin 0.0096 0.0017 0.0142 0.004 0.007 0.0025 0.0082 0.0024 0.0121 0.0004 0.0162 0.0008 0.039 0.0002 0.0005 0.0001 0.0062 0.0002
Bud 0.0008 0.0026 0.0009 0.0003 0.0009 0.0009 0.0007 0.0054 0.002 0.0005 0.0003 0.0001 0.001 0.0005 0.0004 0.0001 0.0012 0.0001
Bud Neck 0.0012 0.0002 0.0006 0.0005 0.0013 0.0013 0.001 0.0004 0.0039 0.0013 0.0008 0.001 0.0092 0.0002 0.0026 0.0003 0.0013 0.0006
Bud Periphery 0.0004 0.0005 0.0004 0.0003 0.001 0.0003 0.0009 0.0019 0.0014 0.0001 0.0006 0.0001 0.0012 0.0002 0.0003 0 0.0009 0
Bud Site 0.0017 0.0022 0.0011 0.0011 0.0018 0.0001 0.0008 0.0041 0.0035 0.0002 0.0008 0 0.0082 0.0146 0.0019 0 0.005 0
Cell Periphery 0.0001 0.0001 0 0.0001 0.0001 0 0.0002 0.0001 0.0001 0 0.0002 0 0.0014 0.0002 0.0002 0 0.0001 0
Cytoplasm 0.844 0.9499 0.897 0.8462 0.8291 0.9016 0.8633 0.9502 0.8806 0.9105 0.7318 0.8965 0.7781 0.9711 0.9573 0.981 0.8498 0.9687
Cytoplasmic Foci 0.0161 0.0021 0.0039 0.0106 0.0075 0.0044 0.014 0.0022 0.0125 0.0167 0.017 0.0021 0.0325 0.0009 0.0054 0.0004 0.0195 0.0005
Eisosomes 0.0001 0 0.0001 0.0001 0.0001 0 0.0001 0 0.0001 0 0.0005 0 0.0002 0 0 0 0 0
Endoplasmic Reticulum 0.0062 0.0009 0.002 0.0017 0.0069 0.0036 0.0066 0.0016 0.0025 0.0016 0.0041 0.0019 0.0039 0.0004 0.0006 0.0002 0.0022 0.0005
Endosome 0.0184 0.0007 0.0041 0.0152 0.025 0.0075 0.0114 0.0006 0.011 0.0123 0.0246 0.0056 0.0288 0.0002 0.0045 0.0001 0.0423 0.0002
Golgi 0.0038 0.0001 0.0016 0.0097 0.0039 0.0029 0.0031 0.0001 0.0037 0.0006 0.0111 0.0015 0.011 0 0.0016 0 0.0153 0
Lipid Particles 0.0036 0 0.0009 0.0123 0.0061 0.0011 0.0026 0 0.0046 0.0016 0.0418 0.0043 0.0092 0 0.0011 0 0.0065 0
Mitochondria 0.0034 0.0002 0.0029 0.0398 0.0145 0.0017 0.0056 0.0004 0.0057 0.0003 0.0688 0.0049 0.0056 0.0001 0.0016 0.0001 0.0034 0.0001
None 0.0043 0.0013 0.0019 0.0015 0.0025 0.0017 0.0073 0.0019 0.0021 0.0017 0.0025 0.0007 0.0033 0.0005 0.0007 0.0002 0.0009 0.0003
Nuclear Periphery 0.0116 0.0033 0.0051 0.0046 0.0198 0.0077 0.016 0.0033 0.0055 0.0043 0.0258 0.0102 0.0081 0.0011 0.0019 0.0016 0.005 0.0021
Nucleolus 0.0006 0 0.0001 0.0001 0.0006 0.0001 0.0003 0.0001 0.0006 0.0002 0.0006 0.0001 0.0004 0.0001 0.0003 0 0.0004 0
Nucleus 0.0575 0.0326 0.0565 0.0359 0.0582 0.0581 0.0417 0.0228 0.0366 0.0435 0.0263 0.0653 0.0427 0.0086 0.0141 0.0156 0.0298 0.0258
Peroxisomes 0.0064 0.0001 0.0016 0.012 0.0029 0.0012 0.0026 0.0002 0.0038 0.0003 0.0162 0.0015 0.0055 0 0.0009 0 0.0029 0
Punctate Nuclear 0.0055 0.001 0.0039 0.0018 0.0043 0.0015 0.0056 0.0016 0.0042 0.0016 0.0042 0.002 0.0029 0.0002 0.0008 0.0002 0.0048 0.0003
Vacuole 0.0036 0.0004 0.0008 0.001 0.0042 0.0013 0.0065 0.0007 0.0028 0.0022 0.0021 0.0008 0.0068 0.0007 0.0029 0.0002 0.002 0.0003
Vacuole Periphery 0.0011 0.0001 0.0003 0.0011 0.0025 0.0005 0.0013 0.0001 0.0008 0.0002 0.0039 0.0005 0.0011 0.0001 0.0004 0.0001 0.0007 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 76.6253 72.75 64.4999 45.72 78.7933 36.971 43.4657 21.277 29.9537 53.493
Translational Efficiency 1.6078 1.9232 1.7439 1.6142 1.4378 1.8641 1.7253 1.9714 1.3924 1.6036

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1313 892 1822 1193 1999 1794 200 1468 3312 2686 2022 2661

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1511.02 1659.71 2564.84 2074.33 1513.54 1628.50 2322.98 1981.63 1512.54 1638.86 2540.92 2023.19
Standard Deviation 387.68 406.10 564.06 517.21 331.46 310.79 455.55 472.30 354.82 345.68 558.96 495.09
Intensity Change Log 2 — 0.135408 0.763346 0.457123 — 0.105617 0.618050 0.388761 — 0.120577 0.692466 0.423318

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000229 0.000221 0.000314 0.000814 0.000180 0.000299 0.000497 0.001004 0.000199 0.000273 0.000332 0.000918
Bud Neck 0.000468 0.000610 0.000749 0.005520 0.001159 0.000738 0.002660 0.008204 0.000885 0.000695 0.000938 0.007001
Bud Site 0.002739 0.002731 0.004639 0.024912 0.004639 0.003035 0.003015 0.038311 0.003886 0.002934 0.004478 0.032304
Cell Periphery 0.000141 0.000127 0.000130 0.000391 0.000308 0.000070 0.000458 0.000369 0.000242 0.000089 0.000163 0.000379
Cytoplasm 0.837861* 0.787417* 0.717623* 0.546148* 0.805135* 0.818726* 0.502534* 0.628339* 0.818109* 0.808328* 0.696349* 0.591490*
Cytoplasmic Foci 0.011054 0.012354 0.003231 0.012148 0.009548 0.012699 0.012810 0.018054 0.010145 0.012584 0.004179 0.015406
Eisosomes 0.000003 0.000003 0.000001 0.000010 0.000003 0.000004 0.000006 0.000010 0.000003 0.000004 0.000002 0.000010
Endoplasmic Reticulum 0.000413 0.000247 0.000042 0.000388 0.000503 0.000345 0.000335 0.000389 0.000467 0.000313 0.000071 0.000389
Endosome 0.000352 0.000570 0.000287 0.009197 0.000358 0.001126 0.000441 0.009449 0.000355 0.000942 0.000302 0.009336
Golgi 0.000594 0.000557 0.000478 0.004601 0.000319 0.001519 0.000488 0.011988 0.000428 0.001200 0.000479 0.008677
Lipid Particles 0.000303 0.000318 0.000283 0.001486 0.000364 0.000540 0.000993 0.000819 0.000340 0.000466 0.000353 0.001118
Mitochondria 0.000912 0.000150 0.000175 0.011367 0.000151 0.000241 0.000257 0.004352 0.000453 0.000211 0.000183 0.007497
Mitotic Spindle 0.003326 0.003321 0.001756 0.063696 0.000917 0.008643 0.002218 0.051811 0.001872 0.006876 0.001802 0.057140
None 0.011023 0.014811 0.010974 0.011169 0.011202 0.011522 0.006782 0.016216 0.011131 0.012614 0.010559 0.013953
Nuclear Periphery 0.000062 0.000097 0.000084 0.000455 0.000096 0.000110 0.000496 0.000298 0.000083 0.000105 0.000125 0.000368
Nuclear Periphery Foci 0.002930 0.002990 0.000432 0.002715 0.001706 0.002055 0.003056 0.001100 0.002191 0.002366 0.000692 0.001824
Nucleolus 0.000106 0.000362 0.000068 0.000592 0.000102 0.000122 0.000171 0.000479 0.000104 0.000202 0.000078 0.000530
Nucleus 0.117309 0.161175* 0.252293* 0.263175* 0.153707* 0.128189 0.432023* 0.175595* 0.139277* 0.139143* 0.270070* 0.214860*
Peroxisomes 0.000042 0.000071 0.000043 0.002565 0.000032 0.000140 0.000044 0.001320 0.000036 0.000117 0.000043 0.001878
Vacuole 0.009518 0.011308 0.006192 0.030924 0.009123 0.009577 0.030595 0.029052 0.009280 0.010152 0.008606 0.029891
Vacuole Periphery 0.000613 0.000559 0.000205 0.007728 0.000448 0.000301 0.000121 0.002841 0.000513 0.000387 0.000196 0.005032

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.83 -0.03 -4.68 -3.55 -2.55 -1.68 -7.74 -2.62 -0.90 3.76 -1.78 -1.21 -4.50 -2.13 -1.55
Bud Neck -3.28 -5.25 -11.93 -10.01 -9.64 1.18 -7.19 -4.92 -6.11 3.81 0.17 -3.99 -9.29 -10.19 -7.09
Bud Site -0.85 -1.15 -4.81 -4.47 -3.59 1.79 -2.68 -3.75 -4.89 -1.78 1.30 -0.36 -5.72 -6.57 -4.71
Cell Periphery -0.75 -3.01 -7.58 -6.87 -5.98 4.40 -5.91 1.47 -5.58 7.43 4.19 -1.06 -2.22 -9.42 -1.69
Cytoplasm 9.10 22.94 37.66 26.65 19.08 -0.48 25.01 26.67 26.42 -11.21 5.08 27.22 44.22 38.28 13.61
Cytoplasmic Foci -2.41 6.37 0.19 2.54 -6.08 -4.23 -4.92 -0.16 3.61 4.80 -4.74 3.94 -0.10 4.35 -3.68
Eisosomes -1.68 3.38 -10.04 -8.43 -13.97 -0.93 -7.77 -7.08 -2.27 4.79 -1.30 1.01 -12.00 -4.74 -13.46
Endoplasmic Reticulum 1.83 5.98 3.08 1.35 -8.55 1.49 0.02 3.96 2.51 5.34 2.01 6.80 4.88 2.71 -6.03
Endosome -1.21 2.27 -2.56 -1.98 -2.99 -2.90 -4.26 -2.85 -1.04 -1.67 -3.15 1.73 -3.83 -1.85 -4.21
Golgi 0.36 1.58 -0.70 -0.91 -1.83 -1.93 -4.71 -2.13 -0.48 -1.55 -1.77 0.53 -2.29 -0.47 -2.40
Lipid Particles -1.61 -2.79 -10.89 -9.93 -9.84 -1.28 -6.95 -9.24 -5.63 3.72 -1.70 -5.08 -14.06 -9.86 -10.06
Mitochondria 0.97 0.93 -0.71 -1.79 -1.75 -0.71 -4.98 -2.20 -1.88 -1.56 0.80 0.84 -1.46 -2.35 -2.40
Mitotic Spindle -0.21 1.57 -5.49 -5.35 -6.26 -4.11 -3.59 -7.85 -4.15 -5.27 -3.68 -0.32 -9.50 -6.23 -9.41
None -1.63 0.88 3.05 4.82 4.76 0.39 5.79 0.84 0.44 -5.74 -0.68 2.22 2.72 3.57 0.85
Nuclear Periphery -6.37 -9.28 -19.28 -17.32 -16.80 -2.88 -11.06 -20.62 -18.38 4.67 -5.15 -11.25 -28.03 -24.85 -18.51
Nuclear Periphery Foci -0.98 9.49 2.98 3.51 -10.03 -2.17 -5.72 0.51 2.96 6.24 -1.98 7.57 2.12 4.17 -7.31
Nucleolus -0.97 1.20 -3.74 0.20 -4.82 -1.42 -10.09 -6.80 -3.84 -1.70 -1.30 0.99 -7.20 -1.07 -7.98
Nucleus -8.23 -26.84 -21.21 -12.00 1.80 5.17 -16.41 -5.97 -10.56 13.84 -1.05 -28.39 -18.23 -17.11 11.19
Peroxisomes -1.74 1.56 -1.64 -1.50 -1.71 -2.76 -5.77 -3.33 -1.22 -2.00 -3.07 0.20 -2.57 -1.75 -2.58
Vacuole -3.29 -2.71 -22.77 -20.57 -22.15 -4.97 -9.07 -24.17 -22.18 -2.69 -5.91 -8.22 -33.23 -30.37 -28.08
Vacuole Periphery 0.29 1.75 -0.65 -0.77 -1.34 0.93 1.57 0.02 -0.97 -1.67 0.86 2.30 -0.62 -1.11 -1.78
Standard name
Human Ortholog
Description Adenine deaminase (adenine aminohydrolase); converts adenine to hypoxanthine; involved in purine salvage; transcriptionally regulated by nutrient levels and growth phase; Aah1p degraded upon entry into quiescence via SCF and the proteasome
Localization
Cell Percentages cytoplasm (96%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Aah1

Aah1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Aah1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available