ORF
Human Ortholog
Description Protein of unknown function; expressed at both mRNA and protein levels
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.96 0.95 0.96 0.94 – 0.83 0.7 0.71 0.56 0.6 0.98 0.98 0.98 0.91 0.93 0.98 0.94 0.96 0.92 0.81 0.86 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.1 0.06 0.22 – 0.31 0.63 0.59 0.65 0.67 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0 2 0 0 0 1 1
Bud 0 0 1 0 1 3 – 2 3 0 3 0 0 0 0 0 0 0 3 0 0 2 3 5
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 1 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 2 1 0 – 1 0 1 0 2 0 0 1 1 2 3 0 0 0 0 0 0
Cytoplasm 423 183 47 120 153 137 – 232 124 150 92 111 183 234 238 64 43 44 407 181 69 64 178 201
Endoplasmic Reticulum 0 2 0 0 2 0 – 1 0 0 2 0 1 1 1 0 3 1 1 0 0 1 5 2
Endosome 0 0 0 1 1 0 – 1 0 0 1 0 0 1 0 0 2 0 3 0 1 1 1 0
Golgi 1 0 0 0 0 0 – 0 0 0 0 0 0 0 0 2 0 0 2 0 0 1 3 0
Mitochondria 4 1 1 12 10 32 – 86 112 125 106 124 0 0 1 3 0 0 2 0 0 0 0 1
Nucleus 1 1 0 0 1 0 – 4 3 3 0 3 0 3 0 2 0 1 1 0 0 0 1 2
Nuclear Periphery 0 0 0 1 0 0 – 2 0 0 0 3 0 1 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 1 1 0 0 – 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 1
Vac/Vac Membrane 0 1 0 0 5 0 – 3 7 4 0 4 2 1 4 4 2 1 2 1 0 2 3 2
Unique Cell Count 428 187 49 126 160 146 280 177 211 163 186 186 238 242 70 46 45 433 190 76 79 207 228
Labelled Cell Count 430 188 50 137 174 172 332 249 284 206 248 187 241 245 76 52 50 433 190 76 79 207 228


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.6 8.3 4.5 5.3 5.6 4.2 – 4.1 3.4 3.5 3.2 3.3 7.9 8.4 8.6 10.4 9.1 8.8 6.5 9.3 9.1
Std Deviation (1e-4) 1.1 1.4 1.0 1.7 1.8 1.2 – 1.4 1.7 1.2 1.0 1.1 2.4 1.8 2.1 3.4 2.5 2.1 1.8 2.6 2.2
Intensity Change (Log2) – – – 0.23 0.31 -0.12 – -0.15 -0.41 -0.37 -0.49 -0.45 0.8 0.9 0.93 1.2 1.01 0.96 0.51 1.04 1.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.2 -0.1 -0.5 – -2.3 -3.7 -3.7 -5.1 -4.8 1.1 1.1 1.1 -1.0 -0.5 0.5
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 10.4383 14.8499 12.4267 11.0469 10.8322 12.7814 9.8458 13.1317 11.6115 9.8983 8.9704 11.6187 12.2587 16.6762 15.0951 14.7513 13.7856 14.8709
Actin 0.0124 0.0002 0.0082 0.0027 0.0009 0.0002 0.0097 0.0002 0.0004 0.0023 0.0144 0.0001 0.0321 0.0002 0.0033 0.0022 0.0042 0.0101
Bud 0.0004 0.0001 0.0002 0.0002 0.0002 0.0001 0.0005 0 0.0002 0.0001 0.0008 0.0001 0.0006 0 0.0001 0.0001 0.0001 0.0001
Bud Neck 0.0012 0.0002 0.0005 0.0008 0.0004 0.0011 0.001 0.0001 0.001 0.0006 0.0015 0.0006 0.0031 0.0001 0.0003 0.0006 0.0006 0.0008
Bud Periphery 0.0004 0 0.0001 0.0001 0.0001 0 0.0004 0 0.0002 0 0.0007 0 0.0011 0 0 0.0001 0 0.0001
Bud Site 0.0116 0.0001 0.0005 0.0002 0.0006 0 0.0009 0.0001 0.0011 0.0002 0.0027 0 0.0035 0.0001 0.0002 0.0006 0.0004 0
Cell Periphery 0.0003 0 0 0.0001 0 0 0.0001 0 0.0001 0 0.0002 0 0.0008 0 0 0 0 0
Cytoplasm 0.8383 0.9918 0.9235 0.9084 0.839 0.9655 0.8807 0.9897 0.9473 0.9342 0.7313 0.9698 0.8533 0.9882 0.9704 0.9551 0.862 0.9567
Cytoplasmic Foci 0.0208 0.0006 0.0122 0.021 0.0081 0.002 0.0298 0.0006 0.0066 0.0173 0.049 0.0013 0.0085 0.0005 0.0017 0.006 0.0085 0.0089
Eisosomes 0.0005 0 0 0 0 0 0.0001 0 0 0 0.0001 0 0.0018 0 0 0 0 0.0002
Endoplasmic Reticulum 0.0038 0.0004 0.0009 0.0053 0.0021 0.0022 0.0029 0.0004 0.0016 0.0011 0.001 0.0009 0.0056 0.0003 0.0015 0.001 0.005 0.0011
Endosome 0.0255 0.0001 0.0129 0.026 0.0701 0.0081 0.0169 0.0001 0.0114 0.0032 0.0605 0.0006 0.0139 0.0001 0.0035 0.007 0.0338 0.0014
Golgi 0.0122 0 0.0102 0.0196 0.0165 0.0018 0.0064 0 0.0021 0.005 0.0467 0 0.0055 0 0.0005 0.0017 0.0045 0.0009
Lipid Particles 0.0123 0 0.0033 0.0018 0.0036 0.0001 0.0051 0 0.0042 0.0027 0.0239 0 0.0105 0 0.0007 0.0084 0.0157 0.0009
Mitochondria 0.0095 0 0.0024 0.0007 0.0343 0.0004 0.0138 0 0.0023 0.0011 0.0187 0.0001 0.0042 0 0.0003 0.0003 0.0008 0.0003
None 0.0077 0.0005 0.0006 0.0008 0.0009 0.0006 0.0051 0.0006 0.001 0.0009 0.0007 0.0006 0.006 0.0005 0.0024 0.0012 0.0036 0.001
Nuclear Periphery 0.0097 0.0006 0.0011 0.002 0.0058 0.0025 0.0035 0.0008 0.0019 0.002 0.0012 0.002 0.0104 0.0009 0.0032 0.0016 0.014 0.0018
Nucleolus 0.0011 0 0 0 0.0002 0 0.0001 0 0.0012 0 0.0002 0 0.002 0 0.0001 0.0001 0.0004 0
Nucleus 0.0166 0.0052 0.007 0.0078 0.0115 0.014 0.009 0.007 0.01 0.0136 0.0103 0.023 0.0212 0.0088 0.0094 0.0095 0.0327 0.011
Peroxisomes 0.0049 0 0.0145 0.0006 0.0005 0 0.0104 0 0.0009 0.0142 0.0304 0 0.0049 0 0.0002 0.0014 0.0023 0.0029
Punctate Nuclear 0.0058 0.0001 0.001 0.0007 0.0012 0.0005 0.0017 0.0001 0.0029 0.0009 0.0014 0.0004 0.0082 0.0003 0.0018 0.0024 0.0081 0.0014
Vacuole 0.0038 0.0001 0.0005 0.0009 0.0024 0.0005 0.0012 0.0001 0.003 0.0003 0.0034 0.0003 0.0019 0.0001 0.0002 0.0004 0.0024 0.0002
Vacuole Periphery 0.0011 0 0.0002 0.0003 0.0018 0.0003 0.0007 0 0.0007 0.0001 0.0011 0.0001 0.0009 0 0.0001 0.0001 0.0008 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 242.8613 604.877 659.299 21.9451 413.1666 1111.2119 75.4972 170.4226 211.8928 24.9278
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1492 471 1128 514 74 1752 635 1638 1566 2223 1763 2152

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 697.51 1179.97 1626.25 1589.79 688.56 1023.05 1369.68 1475.22 697.09 1056.30 1533.84 1502.58
Standard Deviation 131.29 271.12 256.55 250.73 96.90 189.42 220.52 230.16 129.88 219.00 273.49 240.25
Intensity Change Log 2 — 0.758464 1.221263 1.188550 — 0.571222 0.992185 1.099276 — 0.668482 1.112004 1.144892

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000106 0.000817 0.000239 0.000515 0.000647 0.000451 0.000376 0.000759 0.000132 0.000529 0.000288 0.000701
Bud Neck 0.005010 0.001632 0.001223 0.003745 0.018591 0.002836 0.001935 0.004109 0.005652 0.002581 0.001479 0.004022
Bud Site 0.005796 0.010140 0.002576 0.031246 0.023910 0.009377 0.004800 0.026035 0.006652 0.009538 0.003377 0.027279
Cell Periphery 0.000158 0.000088 0.000128 0.000121 0.000401 0.000077 0.000103 0.000091 0.000169 0.000079 0.000119 0.000098
Cytoplasm 0.664180* 0.804431* 0.629702* 0.596894* 0.436939* 0.779027* 0.660739* 0.618565* 0.653442* 0.784410* 0.640881* 0.613389*
Cytoplasmic Foci 0.098843 0.053542 0.007779 0.008586 0.351201* 0.073404 0.007818 0.027260 0.110768 0.069195 0.007793 0.022800
Eisosomes 0.000053 0.000016 0.000004 0.000009 0.000266 0.000023 0.000010 0.000012 0.000063 0.000022 0.000006 0.000012
Endoplasmic Reticulum 0.001211 0.000493 0.000349 0.000604 0.002507 0.000551 0.000793 0.000378 0.001272 0.000539 0.000509 0.000432
Endosome 0.007357 0.003864 0.000486 0.005046 0.017046 0.006527 0.000915 0.026968 0.007815 0.005963 0.000641 0.021732
Golgi 0.001025 0.002439 0.000166 0.001353 0.007478 0.002802 0.000154 0.024786 0.001330 0.002725 0.000162 0.019189
Lipid Particles 0.003153 0.000530 0.000540 0.000834 0.010499 0.001474 0.000633 0.001674 0.003500 0.001274 0.000573 0.001473
Mitochondria 0.003198 0.000244 0.000246 0.005244 0.015852 0.003463 0.000370 0.012239 0.003796 0.002781 0.000291 0.010568
Mitotic Spindle 0.001187 0.008641 0.007557 0.061430 0.003398 0.008483 0.019185 0.088451 0.001291 0.008516 0.011745 0.081997
None 0.015615 0.007297 0.008705 0.010590 0.001403 0.002968 0.008662 0.006109 0.014944 0.003885 0.008689 0.007179
Nuclear Periphery 0.000347 0.000163 0.000391 0.002912 0.000303 0.000135 0.000602 0.000547 0.000344 0.000141 0.000467 0.001112
Nuclear Periphery Foci 0.001213 0.002635 0.002009 0.002698 0.001038 0.001351 0.003266 0.002117 0.001205 0.001623 0.002462 0.002256
Nucleolus 0.002065 0.000353 0.000106 0.000382 0.000934 0.000334 0.000110 0.000295 0.002012 0.000338 0.000107 0.000316
Nucleus 0.131066* 0.061234 0.317848* 0.145614* 0.065205 0.046491 0.226062* 0.068521 0.127953 0.049615 0.284788* 0.086934
Peroxisomes 0.001035 0.001074 0.000049 0.000256 0.019462 0.001752 0.000562 0.002345 0.001906 0.001609 0.000234 0.001846
Vacuole 0.056545 0.039884 0.019824 0.106517 0.021154 0.057567 0.062819 0.070831 0.054872 0.053821 0.035310 0.079355
Vacuole Periphery 0.000839 0.000484 0.000073 0.015404 0.001764 0.000905 0.000085 0.017906 0.000883 0.000816 0.000078 0.017309

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.38 -8.82 -5.45 0.97 -0.75 0.67 0.65 1.16 0.89 1.62 -3.13 -7.68 -4.38 1.21 1.59
Bud Neck 4.19 3.02 3.13 -2.66 0.27 4.11 4.10 4.35 2.14 1.67 3.95 3.86 4.83 1.42 2.52
Bud Site -2.76 1.43 -2.15 -0.39 -2.92 1.10 1.36 1.21 1.09 -1.63 -1.99 1.56 -1.40 0.69 -3.93
Cell Periphery 1.65 -1.79 -0.75 -6.51 2.31 6.23 4.99 6.32 0.50 7.37 2.57 -0.72 2.03 -3.09 10.00
Cytoplasm -9.82 10.00 11.72 18.15 4.48 -10.40 -3.60 -5.67 18.31 -5.92 -14.52 10.29 6.76 24.59 -3.96
Cytoplasmic Foci 9.08 25.75 24.97 9.97 -1.27 10.88 13.43 13.36 21.62 -1.81 9.81 27.24 26.93 23.69 -1.17
Eisosomes 7.98 10.80 9.21 1.61 -8.17 8.46 8.70 8.92 9.26 6.34 9.22 11.97 11.93 9.16 -0.45
Endoplasmic Reticulum 1.79 1.69 2.31 2.76 4.62 1.09 0.85 1.29 5.10 10.64 1.94 1.47 2.86 5.55 12.71
Endosome 4.92 10.99 10.20 4.04 -0.73 3.46 5.16 4.51 3.72 -2.57 2.98 11.75 7.07 4.52 -2.87
Golgi -0.99 3.47 3.33 1.83 0.02 1.49 2.24 1.55 0.24 -2.82 -1.86 4.20 -0.78 0.77 -2.76
Lipid Particles 5.93 4.20 1.84 -9.09 -5.17 4.74 4.90 4.95 0.93 0.78 4.77 5.31 5.03 -0.64 -0.93
Mitochondria 3.05 2.94 2.58 -1.33 -0.94 2.51 3.11 2.92 1.88 -2.10 1.05 3.62 2.66 1.81 -2.23
Mitotic Spindle -2.08 -4.90 -4.27 -1.77 -1.65 -1.44 -2.86 -3.93 -3.51 -0.33 -4.71 -6.31 -8.30 -3.93 -2.18
None 3.48 4.50 4.93 0.08 1.75 -2.96 -10.31 -9.16 -8.38 3.78 6.67 4.25 5.39 -4.32 4.73
Nuclear Periphery 2.14 -9.61 -3.58 -4.20 -1.13 1.54 -7.14 -3.60 -17.91 7.77 5.43 -13.66 -7.33 -11.71 2.65
Nuclear Periphery Foci -4.25 -6.59 -6.14 0.71 1.25 -0.57 -3.71 -0.50 0.27 6.80 -2.88 -8.72 -2.87 0.41 7.34
Nucleolus 4.72 6.12 6.11 0.79 -0.08 2.94 3.86 3.58 1.51 -2.30 5.60 6.25 6.11 1.69 -1.98
Nucleus 10.07 -26.72 -0.20 -9.01 24.18 1.61 -15.50 -3.92 -19.81 20.36 16.73 -27.97 3.20 -21.49 36.54
Peroxisomes 0.49 6.18 6.02 3.05 -1.32 3.66 3.95 3.94 3.70 -0.08 1.15 6.05 5.60 4.26 -1.17
Vacuole -0.04 0.17 -19.77 -18.55 -19.57 -11.08 -14.50 -30.44 -24.10 -8.68 -2.25 -6.65 -30.91 -30.03 -23.94
Vacuole Periphery 1.92 7.96 -0.48 -0.98 -1.46 2.62 6.04 0.15 -1.34 -2.69 0.61 8.80 -1.40 -1.57 -3.03
ORF
Human Ortholog
Description Protein of unknown function; expressed at both mRNA and protein levels
Localization
Cell Percentages cytoplasm (64%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YNL143C

YNL143C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ynl143c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available