ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNL146W is not an essential gene
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 – – – – – –
Cell Periphery 0 0 0.07 0 0 0 – 0 0 0 0 0 0 0 0.05 – – 0.19 0 0 0 0 0 0
Cytoplasm 0.62 0.64 0.74 0.76 0.73 0.5 – 0.54 0.44 0.5 0.49 0.53 0.85 0.75 0.81 – – 0.62 0.25 0.35 0.45 0.43 0.48 0.51
Endoplasmic Reticulum 0.31 0.32 0.15 0.05 0 0.06 – 0 0 0 0 0 0.15 0.25 0.16 – – 0 0.55 0.42 0.32 0.33 0.28 0.22
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.28 0.18 0.52 – 0.43 0.6 0.5 0.58 0.55 0 0 0 – – 0 0.08 0.1 0.14 0.14 0.1 0.07
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0.06 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0 0 0 0.11 0.11 – 0.13 0.1 0.1 0 0.06 0 0 0 – – 0.12 0.07 0.06 0 0.07 0.06 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 0 0 1 2 0 – 1 1 2 0 1 0 2 2 – – 0 3 3 0 0 5 7
Bud 1 1 2 6 12 11 – 33 20 25 20 25 1 1 3 – – 0 2 2 0 0 3 12
Bud Neck 0 0 0 0 1 0 – 0 0 0 0 0 0 1 0 – – 0 3 2 0 0 2 8
Bud Site 0 0 0 0 0 2 – 1 2 5 2 8 0 0 0 – – 0 – – – – – –
Cell Periphery 0 1 3 1 4 7 – 1 5 7 5 14 1 15 27 – – 3 1 1 1 0 3 8
Cytoplasm 165 130 34 293 426 235 – 516 261 372 215 340 174 319 418 – – 10 87 89 43 28 140 232
Endoplasmic Reticulum 83 64 7 19 14 26 – 5 25 15 10 14 31 106 83 – – 0 194 107 30 22 81 102
Endosome 0 0 0 1 13 4 – 9 0 3 0 4 0 0 2 – – 0 1 2 1 0 2 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 1 1 1 – – 0 0 0 0 0 0 1
Mitochondria 7 4 1 110 105 244 – 406 359 367 254 352 2 0 1 – – 0 28 26 13 9 30 30
Nucleus 3 0 1 0 2 3 – 27 9 31 8 17 1 0 0 – – 1 0 0 0 0 0 1
Nuclear Periphery 0 0 0 0 1 4 – 18 5 5 3 7 0 0 0 – – 0 2 1 0 0 0 0
Nucleolus 0 0 0 1 2 1 – 3 1 1 0 1 0 0 0 – – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 2 0 – 3 0 1 2 1 0 0 1 – – 0 0 0 0 0 1 2
Vac/Vac Membrane 19 9 0 8 64 51 – 126 61 73 18 41 5 4 14 – – 2 25 16 3 4 16 42
Unique Cell Count 264 203 46 386 581 469 947 596 737 441 644 205 427 516 16 355 256 95 67 294 459
Labelled Cell Count 281 209 48 441 648 588 1149 749 907 537 825 216 449 552 16 355 256 95 67 294 459


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 5.6 4.2 3.8 4.8 3.8 – 4.3 3.5 4.0 3.5 3.7 5.4 5.7 5.7 – – 10.7 – 5.5 5.7
Std Deviation (1e-4) 0.9 0.6 0.7 0.9 1.5 0.9 – 1.6 0.9 1.1 0.8 1.2 1.1 0.9 1.2 – – 1.8 – 1.0 1.1
Intensity Change (Log2) – – – -0.14 0.21 -0.12 – 0.03 -0.24 -0.05 -0.24 -0.19 0.39 0.46 0.44 – – 1.36 – 0.41 0.45


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 – – 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 – – 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 – – 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 – – 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 – – 0
Cytoplasm 0.3 -0.1 -3.1 – -2.6 -3.9 -3.1 -3.2 -2.8 1.8 0.1 1.2 – – -0.9
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 1.5 0.2 – – 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 – – 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 – – 0
Mitochondria 0 0 0 – 0 0 0 0 0 0 0 0 – – 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 – – 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 – – 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 – – 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 – – 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 – – 0
Vacuole 0 0 0 – 2.6 0 0 0 0 0 0 0 – – 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.005 2.3827 2.254 1.9293 2.1271 2.3669 1.0498 1.4067 1.1297 1.0329 1.2501 1.427 3.0522 3.5372 3.0677 3.3244 2.5024 2.9199
Actin 0.0215 0.0004 0.0017 0.0184 0.0012 0.0006 0.0138 0.0006 0.0181 0.0351 0.0033 0.0023 0.0032 0.0002 0.004 0.0318 0.0294 0.0003
Bud 0.0001 0 0 0.0004 0.0012 0 0.0003 0.0001 0.0014 0.0004 0.0022 0.0002 0.0003 0 0.0001 0.0001 0.0004 0
Bud Neck 0.002 0.0006 0.0003 0.0034 0.0005 0.0012 0.0002 0.0002 0.0007 0.0075 0.0011 0.0011 0.0002 0.0001 0.0002 0.0011 0.0005 0.001
Bud Periphery 0.0002 0 0.0001 0.0004 0.0032 0 0.0005 0.0001 0.0026 0.0008 0.0089 0.0006 0.0003 0.0001 0.0002 0.0001 0.0006 0
Bud Site 0.0011 0.0087 0.0007 0.0044 0.002 0.0001 0.001 0.0019 0.0074 0.0024 0.0008 0.0004 0.0006 0.0011 0.0005 0.0003 0.0018 0.0001
Cell Periphery 0.0005 0.0011 0.0007 0.0005 0.0004 0.0003 0.0006 0.001 0.001 0.0014 0.0013 0.0002 0.0005 0.001 0.0009 0.0005 0.0004 0.0002
Cytoplasm 0.2662 0.3231 0.3277 0.1339 0.3523 0.4101 0.2508 0.3663 0.2837 0.1989 0.3194 0.219 0.1899 0.3342 0.3374 0.304 0.3775 0.3882
Cytoplasmic Foci 0.0083 0.0051 0.0094 0.0379 0.0097 0.0065 0.0139 0.0109 0.0188 0.034 0.0123 0.0138 0.0611 0.0029 0.0066 0.0064 0.007 0.0121
Eisosomes 0.0003 0.0001 0.0001 0.0002 0 0 0.0003 0.0001 0.0002 0.0003 0 0.0001 0.0001 0 0.0005 0.0001 0.0001 0
Endoplasmic Reticulum 0.5078 0.497 0.5139 0.5578 0.4346 0.4803 0.4807 0.4804 0.4949 0.4013 0.3959 0.6294 0.5473 0.5341 0.5382 0.5323 0.5189 0.5312
Endosome 0.024 0.0067 0.0189 0.0216 0.0134 0.0127 0.0406 0.0208 0.0369 0.0663 0.0941 0.0297 0.0227 0.0052 0.0092 0.0257 0.0063 0.0068
Golgi 0.0037 0.0004 0.0021 0.0028 0.0006 0.0004 0.0097 0.0011 0.0058 0.0542 0.0858 0.0124 0.0064 0.0003 0.0017 0.0079 0.0038 0.0004
Lipid Particles 0.0064 0.0034 0.0097 0.0032 0.0023 0.0023 0.045 0.0124 0.0143 0.0804 0.0046 0.0046 0.0271 0.0021 0.006 0.0203 0.0069 0.0014
Mitochondria 0.0017 0.0001 0.0007 0.0016 0.0057 0.0003 0.0078 0.0005 0.0326 0.0173 0.0139 0.0099 0.0051 0.0002 0.0128 0.0009 0.0045 0.0003
None 0.1055 0.1226 0.0791 0.1554 0.0992 0.0444 0.1003 0.0726 0.0463 0.0431 0.0126 0.0381 0.0734 0.0997 0.0665 0.042 0.0065 0.0368
Nuclear Periphery 0.0301 0.0186 0.0224 0.0377 0.0209 0.0208 0.0175 0.0198 0.0175 0.0167 0.0109 0.0203 0.0144 0.009 0.0092 0.0144 0.0068 0.0114
Nucleolus 0.0005 0.0002 0.0002 0.0005 0.0086 0.0002 0.0004 0.0001 0.0003 0.0029 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001
Nucleus 0.0062 0.0055 0.0047 0.0118 0.0291 0.0121 0.0027 0.0034 0.0029 0.0032 0.0039 0.0068 0.0028 0.0039 0.0017 0.003 0.0023 0.0048
Peroxisomes 0.001 0 0.0002 0.001 0.0004 0.0001 0.0051 0.0001 0.0043 0.0234 0.0005 0.0035 0.027 0 0.0005 0.0014 0.0226 0.0002
Punctate Nuclear 0.0054 0.0023 0.0019 0.0035 0.0025 0.004 0.0022 0.0015 0.0014 0.0028 0.0004 0.0009 0.0135 0.0007 0.0005 0.0007 0.0007 0.0019
Vacuole 0.006 0.0035 0.0047 0.0028 0.009 0.0032 0.0052 0.0056 0.0077 0.0061 0.0234 0.0056 0.003 0.0049 0.003 0.0057 0.0028 0.0025
Vacuole Periphery 0.0014 0.0003 0.0008 0.0008 0.0032 0.0004 0.0014 0.0006 0.0013 0.0015 0.0043 0.0012 0.0009 0.0003 0.0004 0.001 0.0002 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression –
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1454 963 1597 940 1781 1025 956 1246 3235 1988 2553 2186

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 617.76 722.49 904.68 903.97 675.83 740.32 860.54 884.35 649.73 731.68 888.15 892.79
Standard Deviation 65.85 122.94 101.57 130.61 84.45 120.26 89.90 117.62 81.91 121.89 99.68 123.75
Intensity Change Log 2 — 0.225931 0.550361 0.549228 — 0.131489 0.348582 0.387957 — 0.177362 0.448468 0.467224

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000077 0.000956 0.000322 0.002453 0.000104 0.000263 0.000652 0.002306 0.000092 0.000599 0.000445 0.002369
Bud Neck 0.008225 0.019010 0.005638 0.007958 0.006122 0.009740 0.005262 0.008690 0.007067 0.014230 0.005497 0.008375
Bud Site 0.001474 0.014714 0.005150 0.029480 0.002088 0.004551 0.011555 0.022010 0.001812 0.009474 0.007549 0.025222
Cell Periphery 0.000285 0.000283 0.000460 0.000872 0.000321 0.000346 0.000590 0.000751 0.000305 0.000315 0.000509 0.000803
Cytoplasm 0.371698* 0.324753* 0.324735* 0.300859* 0.357617* 0.320754* 0.315783* 0.354621* 0.363946* 0.322691* 0.321383* 0.331503*
Cytoplasmic Foci 0.211712* 0.213488* 0.023313 0.060350 0.161638 0.221604* 0.039315 0.068483 0.184144* 0.217673* 0.029305 0.064986
Eisosomes 0.000410 0.000333 0.000096 0.000318 0.000443 0.000421 0.000164 0.000460 0.000428 0.000378 0.000121 0.000399
Endoplasmic Reticulum 0.070258 0.068380 0.175710* 0.126879* 0.105669* 0.079655 0.239799* 0.126599* 0.089753 0.074193 0.199709* 0.126719*
Endosome 0.011231 0.032144 0.007543 0.014113 0.011527 0.027784 0.011632 0.012431 0.011394 0.029896 0.009074 0.013154
Golgi 0.007592 0.009427 0.000558 0.004641 0.006437 0.010091 0.000529 0.002315 0.006956 0.009769 0.000547 0.003315
Lipid Particles 0.119010* 0.050929 0.012016 0.023548 0.111581* 0.093947 0.019782 0.028360 0.114920* 0.073109 0.014924 0.026291
Mitochondria 0.009299 0.010024 0.000987 0.003841 0.006842 0.011712 0.000753 0.001733 0.007947 0.010894 0.000900 0.002639
Mitotic Spindle 0.000234 0.003550 0.000778 0.017637 0.000957 0.003465 0.003990 0.005594 0.000632 0.003506 0.001980 0.010773
None 0.006444 0.005234 0.001440 0.001276 0.002996 0.004075 0.000945 0.001541 0.004546 0.004637 0.001255 0.001427
Nuclear Periphery 0.008863 0.005067 0.039568 0.007675 0.011682 0.009412 0.032975 0.006349 0.010415 0.007308 0.037099 0.006919
Nuclear Periphery Foci 0.003514 0.007866 0.039310 0.037828 0.005881 0.005189 0.047449 0.037244 0.004818 0.006485 0.042358 0.037495
Nucleolus 0.001169 0.002286 0.000309 0.000348 0.001256 0.002488 0.000267 0.000324 0.001217 0.002390 0.000293 0.000334
Nucleus 0.133338* 0.102996 0.295182* 0.104843 0.163592* 0.103412 0.168740* 0.121669 0.149994* 0.103211 0.247834* 0.114434
Peroxisomes 0.004736 0.012516 0.000729 0.001382 0.003829 0.006584 0.000806 0.001722 0.004237 0.009458 0.000758 0.001576
Vacuole 0.028920 0.112905 0.065698 0.251819* 0.037983 0.081266 0.098717 0.196419 0.033910 0.096592 0.078062 0.220242*
Vacuole Periphery 0.001511 0.003139 0.000459 0.001880 0.001434 0.003241 0.000295 0.000380 0.001468 0.003192 0.000397 0.001025

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -8.36 -6.78 -5.16 -3.03 -4.50 -3.89 -3.89 -4.53 -4.23 -3.21 -9.09 -6.12 -6.57 -5.07 -5.34
Bud Neck -5.28 1.90 0.25 5.88 -2.13 -2.83 0.55 -2.23 0.83 -2.54 -6.03 1.63 -1.56 5.03 -3.19
Bud Site -7.56 -4.06 -7.48 -3.01 -6.03 -2.54 -4.55 -7.59 -6.41 -2.72 -7.69 -5.85 -10.67 -6.22 -6.85
Cell Periphery 0.14 -5.21 -4.46 -4.50 -2.65 -0.75 -5.53 -8.04 -7.27 -1.57 -0.45 -7.32 -8.05 -7.82 -3.65
Cytoplasm 4.65 5.87 7.15 2.14 2.29 4.06 4.58 1.64 -2.09 -2.61 6.11 7.27 5.93 -0.19 -0.62
Cytoplasmic Foci -0.18 38.29 28.20 23.85 -11.47 -8.74 27.32 20.73 24.65 -8.37 -6.52 47.75 34.23 34.16 -15.73
Eisosomes 2.01 10.87 3.44 1.31 -7.98 0.55 10.42 0.05 -0.33 -5.14 1.76 15.96 1.51 0.08 -7.88
Endoplasmic Reticulum 0.47 -14.00 -3.38 -3.42 8.62 3.81 -11.82 -0.77 -4.01 10.50 3.36 -17.17 -2.80 -5.39 12.99
Endosome -10.85 4.25 -0.77 9.73 -4.13 -9.27 -0.14 -0.08 8.77 0.06 -14.24 3.45 -0.59 13.15 -3.43
Golgi -1.23 12.59 3.45 3.58 -2.35 -2.59 13.36 5.71 5.49 -2.24 -2.79 18.01 6.32 6.54 -3.20
Lipid Particles 13.53 25.25 22.47 9.65 -5.37 3.44 26.30 23.67 15.59 -3.98 11.55 37.31 32.54 17.86 -7.83
Mitochondria -0.43 9.66 4.84 4.71 -1.72 -2.81 7.68 5.59 6.26 -1.32 -2.58 12.06 7.42 8.00 -2.08
Mitotic Spindle -2.82 -1.84 -5.26 -3.85 -5.05 -1.79 -2.11 -2.75 -0.80 -0.59 -3.10 -2.22 -5.90 -3.54 -4.86
None 0.84 5.42 5.70 3.46 0.12 -1.01 3.36 1.87 2.17 -0.54 -0.20 6.04 5.04 3.82 -0.24
Nuclear Periphery 3.43 -13.04 1.44 -1.92 13.84 1.68 -7.43 5.80 2.68 9.80 3.51 -14.72 5.18 0.94 17.33
Nuclear Periphery Foci -5.24 -17.87 -13.42 -10.72 2.34 1.07 -15.21 -15.04 -15.22 3.41 -3.15 -23.11 -20.19 -18.35 3.69
Nucleolus -2.85 5.68 4.15 5.12 -1.66 -2.11 2.89 2.75 4.67 -1.29 -3.24 4.65 4.17 6.82 -1.89
Nucleus 5.13 -23.69 4.88 -0.44 25.88 10.87 -0.70 7.68 -3.22 6.60 11.53 -18.98 8.92 -2.73 24.90
Peroxisomes -5.05 12.09 10.07 7.42 -2.64 -4.56 11.46 6.88 8.15 -3.69 -6.51 16.31 11.73 10.04 -4.43
Vacuole -16.99 -14.04 -31.59 -19.00 -25.73 -11.13 -14.22 -28.35 -19.67 -14.11 -19.95 -19.17 -41.94 -26.60 -29.48
Vacuole Periphery -5.91 4.75 0.36 3.49 -1.91 -4.25 4.63 4.37 8.25 -0.55 -6.58 5.69 2.95 8.35 -1.68
ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNL146W is not an essential gene
Localization
Cell Percentages ER (60%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

YNL146W

YNL146W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ynl146w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available