Standard name
Human Ortholog
Description Alpha-tubulin folding protein; similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 0.06 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
Bud Neck – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0.96 0.95 0.96 0.94 0.87 0.82 – 0.89 0.76 0.73 0.69 0.88 1.0 0.99 0.92 0.87 0.8 1.0 0.93 0.87 0.9 0.87 0.78
Endoplasmic Reticulum – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – 0 0 0 0.06 0 0.12 – 0.18 0.19 0.27 0.32 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus – 0 0.06 0 0 0.08 0.06 – 0 0.08 0.05 0 0.09 0 0 0 0 0.05 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0.06 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0 0 0 0 0 0.09 – 0.05 0.06 0.13 0.09 0 0 0 0.11 0.17 0.23 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0
Bud – 0 0 0 1 0 1 – 1 2 2 2 0 0 0 0 0 1 0 0 1 0 4 6
Bud Neck – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery – 1 0 0 0 1 0 – 1 0 1 0 0 0 0 4 5 5 0 0 0 0 0 0
Cytoplasm – 297 59 100 15 97 27 – 108 81 76 103 49 51 144 478 312 346 17 293 86 99 128 87
Endoplasmic Reticulum – 1 0 0 0 1 0 – 0 0 0 1 1 0 0 19 9 11 0 0 0 1 0 3
Endosome – 2 0 0 0 0 0 – 0 0 0 1 0 0 0 0 1 2 0 1 0 0 0 1
Golgi – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 1 1 0 1 0 0 0 1
Mitochondria – 1 0 1 1 2 4 – 22 20 28 48 1 0 2 23 9 7 0 1 0 1 2 0
Nucleus – 4 4 1 0 9 2 – 2 9 5 4 5 0 1 13 8 20 0 6 1 2 2 2
Nuclear Periphery – 2 0 0 0 2 2 – 2 1 0 1 0 0 0 1 0 2 0 0 0 0 0 1
Nucleolus – 0 0 1 0 2 0 – 0 1 0 0 1 0 0 0 2 2 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2
Vac/Vac Membrane – 8 1 1 0 5 3 – 6 6 14 13 1 0 2 55 60 100 0 5 1 1 3 1
Unique Cell Count 310 62 104 16 112 33 122 106 104 149 56 51 146 518 360 431 18 316 100 110 148 112
Labelled Cell Count 316 64 104 17 119 39 143 120 126 174 58 51 149 594 408 497 18 316 100 110 148 112


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 6.9 6.2 6.4 4.8 6.0 5.1 – 5.6 5.0 5.5 5.5 5.9 7.1 7.0 7.1 8.6 8.3 8.3 8.4 10.6
Std Deviation (1e-4) – 3.8 1.3 1.7 0.8 1.3 0.8 – 1.6 0.9 1.7 1.5 1.0 1.7 2.0 1.3 1.6 1.6 2.9 2.5 4.1
Intensity Change (Log2) – – – 0.04 -0.38 -0.05 -0.28 – -0.16 -0.32 -0.18 -0.17 -0.07 0.18 0.17 0.19 0.46 0.42 0.42 0.43 0.77


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.3 -0.2 -1.8 -2.1 – -1.5 -3.1 -3.5 -4.1 -1.5 1.6 1.5 -0.8 -1.9 -2.9
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 3.6 3.6 4.5 5.1 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 2.6 0 0 0 0 2.3 3.1 3.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.8496 2.5818 2.3228 2.4158 2.0458 2.235 8.1569 5.5007 6.3439 6.9338 5.2039 7.2603 5.086 5.2981 5.3509 3.4827 4.7328 4.6782
Actin 0.0368 0.0012 0.006 0.0516 0.0017 0.0075 0.0824 0.0146 0.0012 0.0004 0.0664 0.0035 0.0239 0.0004 0.0168 0.001 0.0208 0.0099
Bud 0.003 0.0011 0.0004 0.0017 0.0006 0.0011 0.002 0.0058 0.0001 0.0001 0.007 0.0001 0.0008 0.0018 0.0007 0.0002 0.0022 0.0001
Bud Neck 0.0303 0.0005 0.0096 0.0014 0.0011 0.0027 0.004 0.0006 0.0001 0.0001 0.0019 0.0009 0.0008 0.0001 0.0264 0.0004 0.0028 0.0042
Bud Periphery 0.005 0.0006 0.0004 0.0014 0.0003 0.0009 0.0027 0.0035 0 0 0.0067 0 0.0008 0.0005 0.001 0.0001 0.0045 0.0001
Bud Site 0.0182 0.0075 0.0112 0.0041 0.0002 0.0006 0.0019 0.0252 0.0004 0.0001 0.0023 0 0.009 0.017 0.0289 0.0002 0.0132 0.0002
Cell Periphery 0.0016 0.0004 0.0003 0.0001 0.0003 0.0001 0.0002 0.0006 0.0001 0.0001 0.0002 0 0.0002 0.0001 0.0005 0.0002 0.001 0.0001
Cytoplasm 0.5475 0.7879 0.7608 0.6865 0.7307 0.7978 0.4874 0.5774 0.7498 0.5465 0.818 0.842 0.6136 0.8193 0.6517 0.8475 0.4377 0.7997
Cytoplasmic Foci 0.0313 0.007 0.0083 0.0355 0.0084 0.0191 0.0787 0.0102 0.0123 0.0053 0.014 0.0097 0.0086 0.0047 0.0058 0.0054 0.0152 0.0069
Eisosomes 0.0006 0 0.0001 0.0003 0.0002 0 0.0003 0.0004 0 0 0.0001 0 0.0002 0 0.0001 0.0001 0.0012 0.0002
Endoplasmic Reticulum 0.0123 0.0032 0.0039 0.0049 0.0173 0.0041 0.0139 0.0026 0.0048 0.0094 0.0118 0.0046 0.0095 0.0026 0.0074 0.0123 0.0157 0.0049
Endosome 0.0407 0.004 0.0125 0.0274 0.0304 0.0234 0.0933 0.0091 0.0077 0.0048 0.009 0.0053 0.0208 0.0019 0.0088 0.0038 0.0473 0.0058
Golgi 0.0158 0.0004 0.0021 0.0135 0.0231 0.01 0.019 0.0133 0.0004 0.0002 0.0029 0.0002 0.003 0.0002 0.001 0.0004 0.0073 0.0013
Lipid Particles 0.0123 0.0002 0.0005 0.008 0.0018 0.0041 0.0572 0.0005 0.0004 0.0001 0.0004 0.0001 0.0023 0.0001 0.001 0.0004 0.0094 0.0005
Mitochondria 0.027 0.0003 0.0005 0.003 0.0035 0.0103 0.0031 0.0105 0.0002 0.0002 0.0012 0.0001 0.0019 0.0001 0.0005 0.0003 0.0177 0.0004
None 0.1161 0.1752 0.1735 0.1041 0.1175 0.071 0.0555 0.3179 0.2154 0.4266 0.0427 0.1193 0.2199 0.1439 0.2394 0.1181 0.2557 0.1494
Nuclear Periphery 0.0328 0.0014 0.0021 0.0072 0.0226 0.0099 0.0233 0.0009 0.0023 0.0021 0.0042 0.0019 0.0369 0.0012 0.0039 0.0023 0.0428 0.0023
Nucleolus 0.0045 0.0002 0.0001 0.0005 0.0004 0.0003 0.0013 0.0001 0 0.0001 0.0001 0.0001 0.0005 0 0.0002 0.0001 0.0135 0.0002
Nucleus 0.0254 0.0046 0.0033 0.009 0.0331 0.0239 0.008 0.0028 0.0018 0.0019 0.0037 0.0061 0.0385 0.0047 0.0019 0.0045 0.0618 0.0099
Peroxisomes 0.0062 0.0002 0.0006 0.014 0.0002 0.0025 0.017 0.0009 0.0002 0.0001 0.0022 0.0002 0.0016 0.0001 0.0011 0.0001 0.0021 0.0004
Punctate Nuclear 0.0117 0.0016 0.0008 0.0237 0.0016 0.0063 0.0443 0.0015 0.0013 0.0009 0.0035 0.0051 0.004 0.0004 0.0017 0.0007 0.0079 0.0028
Vacuole 0.0172 0.0024 0.0027 0.0015 0.0031 0.0031 0.0028 0.001 0.001 0.0009 0.0014 0.0007 0.0016 0.0006 0.0009 0.0017 0.0135 0.0006
Vacuole Periphery 0.0037 0.0002 0.0005 0.0006 0.002 0.0012 0.0017 0.0003 0.0003 0.0002 0.0004 0.0001 0.0016 0.0001 0.0003 0.0002 0.0068 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.2118 14.1832 17.222 14.4485 17.7658 10.0909 18.2517 19.7978 27.3285 13.3428
Translational Efficiency 2.5856 1.2807 1.4495 2.333 1.4349 2.5616 1.4675 1.8268 1.1335 1.8185

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
89 190 105 723 733 1269 1387 1310 822 1459 1492 2033

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 711.14 828.40 1020.78 933.76 766.51 868.18 836.59 831.85 760.51 863.00 849.55 868.09
Standard Deviation 115.34 130.44 125.14 140.08 97.35 106.51 127.12 158.78 100.93 110.74 135.44 160.01
Intensity Change Log 2 — 0.220194 0.521466 0.392918 — 0.179690 0.126216 0.118019 — 0.199325 0.329957 0.256862

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000248 0.000459 0.000934 0.007712 0.000046 0.000192 0.000150 0.000953 0.000068 0.000227 0.000205 0.003357
Bud Neck 0.003812 0.007730 0.002320 0.013178 0.001477 0.011460 0.003954 0.003416 0.001730 0.010974 0.003839 0.006888
Bud Site 0.003750 0.016979 0.004071 0.057763 0.001005 0.010185 0.004621 0.010249 0.001302 0.011070 0.004582 0.027146
Cell Periphery 0.000360 0.000114 0.000295 0.000395 0.000106 0.000142 0.000826 0.000445 0.000134 0.000138 0.000788 0.000427
Cytoplasm 0.454531* 0.545286* 0.364639* 0.391079* 0.678640* 0.567809* 0.484001* 0.568055* 0.654375* 0.564876* 0.475601* 0.505117*
Cytoplasmic Foci 0.091019 0.164981 0.014276 0.031484 0.082964 0.120922 0.022297 0.018626 0.083836 0.126660 0.021733 0.023199
Eisosomes 0.000112 0.000063 0.000082 0.000054 0.000046 0.000069 0.000610 0.000056 0.000053 0.000068 0.000573 0.000055
Endoplasmic Reticulum 0.003109 0.001180 0.009524 0.005733 0.000743 0.000737 0.000965 0.000786 0.000999 0.000795 0.001567 0.002545
Endosome 0.004335 0.019027 0.004403 0.034948 0.004179 0.013977 0.002942 0.002957 0.004196 0.014635 0.003045 0.014334
Golgi 0.000236 0.002266 0.000181 0.061037 0.000263 0.006015 0.000922 0.002668 0.000260 0.005527 0.000870 0.023426
Lipid Particles 0.008964 0.003049 0.002530 0.001115 0.002393 0.004371 0.002318 0.000938 0.003105 0.004199 0.002333 0.001001
Mitochondria 0.000840 0.007001 0.000317 0.033555 0.000241 0.008368 0.000982 0.001287 0.000306 0.008190 0.000935 0.012762
Mitotic Spindle 0.000513 0.005986 0.000347 0.080914 0.002589 0.010437 0.005810 0.012954 0.002364 0.009857 0.005426 0.037123
None 0.011469 0.002149 0.001695 0.011573 0.003957 0.002696 0.014175 0.068371 0.004770 0.002625 0.013297 0.048172
Nuclear Periphery 0.001165 0.000386 0.001618 0.003907 0.000486 0.000400 0.000597 0.000435 0.000559 0.000399 0.000669 0.001670
Nuclear Periphery Foci 0.001803 0.002133 0.006960 0.007521 0.001036 0.000435 0.000731 0.001836 0.001119 0.000656 0.001169 0.003858
Nucleolus 0.001724 0.000668 0.000305 0.000639 0.000476 0.001411 0.000781 0.001101 0.000611 0.001315 0.000747 0.000937
Nucleus 0.354359* 0.088555 0.407993* 0.090053 0.176703* 0.156932* 0.388795* 0.245283* 0.195938* 0.148027* 0.390146* 0.190078*
Peroxisomes 0.001447 0.006864 0.000310 0.005804 0.000352 0.004536 0.000607 0.000339 0.000470 0.004839 0.000586 0.002282
Vacuole 0.055770 0.123310 0.176981 0.110943 0.041963 0.076519 0.063099 0.058278 0.043458 0.082613 0.071114 0.077008
Vacuole Periphery 0.000436 0.001814 0.000219 0.050593 0.000334 0.002386 0.000816 0.000967 0.000345 0.002311 0.000774 0.018616

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.56 -5.18 -2.63 -2.15 -1.53 -2.79 -4.26 -2.69 -1.91 -2.07 -3.28 -5.40 -3.77 -3.12 -3.19
Bud Neck -2.07 0.83 -1.37 0.73 -2.65 -7.95 -4.89 -3.74 6.54 1.41 -8.25 -4.36 -4.65 5.78 -0.53
Bud Site -3.48 -0.82 -5.66 -1.44 -5.69 -6.36 -5.01 -6.26 1.38 -2.37 -7.24 -4.86 -8.98 -1.14 -5.74
Cell Periphery 3.46 0.92 2.98 -1.26 3.64 -2.92 -7.24 -4.77 -3.73 4.45 -0.33 -6.98 -3.85 -3.75 5.29
Cytoplasm -2.65 3.33 0.34 4.54 -4.56 9.69 18.17 13.77 4.00 -4.53 7.94 16.93 15.48 7.61 -2.65
Cytoplasmic Foci -3.69 6.24 6.08 11.04 -1.12 -6.55 15.22 16.93 25.31 4.17 -7.53 16.34 18.02 27.60 4.04
Eisosomes 3.48 1.97 5.94 3.23 5.06 -5.33 -5.79 -0.18 5.70 5.78 -3.49 -5.71 2.50 7.19 5.85
Endoplasmic Reticulum 2.67 -4.81 1.18 -2.13 5.97 0.14 -3.08 -3.52 -2.67 0.74 1.27 -4.21 -3.13 -4.08 1.13
Endosome -3.87 -0.09 -2.55 2.55 -2.57 -7.02 2.86 3.85 8.71 1.11 -7.92 2.89 0.13 7.59 -2.14
Golgi -3.20 1.00 -4.23 -3.60 -4.24 -4.66 -1.24 -2.11 3.43 -1.12 -4.85 -1.23 -4.63 -0.08 -4.14
Lipid Particles 2.83 3.30 4.08 2.46 4.67 -2.37 0.64 5.79 4.43 2.11 -1.43 1.77 6.76 4.65 2.13
Mitochondria -1.07 2.88 -3.41 -0.35 -3.66 -4.61 -2.69 -2.49 4.24 -0.03 -4.67 -2.39 -4.03 2.58 -3.28
Mitotic Spindle -1.50 -0.71 -7.32 -5.39 -7.20 -2.98 -1.94 -4.24 -1.02 -2.81 -3.17 -2.05 -7.56 -4.22 -6.24
None 2.67 2.85 0.81 -5.62 -6.52 1.91 -5.09 -10.28 -10.72 -7.68 3.05 -4.31 -10.15 -11.30 -6.86
Nuclear Periphery 3.90 -2.33 -2.26 -4.42 -1.11 1.38 -5.17 -5.79 -9.47 0.33 2.68 -5.08 -5.11 -6.41 -2.67
Nuclear Periphery Foci -0.72 -5.09 -1.98 -0.97 3.76 3.56 0.19 -2.95 -5.52 -3.21 2.53 -1.44 -4.35 -6.45 -3.34
Nucleolus 2.81 3.99 3.53 1.79 -1.97 -2.47 -1.61 -2.79 1.73 0.01 -2.12 -0.66 -0.25 2.05 0.52
Nucleus 9.52 -1.03 7.25 -6.49 9.87 2.38 -19.18 -10.43 -13.92 9.35 5.95 -17.37 -4.23 -12.49 15.56
Peroxisomes -3.12 5.12 0.20 3.07 -2.22 -6.11 -1.08 2.42 6.30 2.14 -6.82 -0.02 -0.82 6.37 -0.70
Vacuole -4.18 -7.03 -12.35 -7.33 -1.50 -6.79 -10.59 -11.62 -6.18 -1.51 -8.08 -11.84 -20.28 -13.37 -7.85
Vacuole Periphery -2.50 2.34 -4.41 -3.85 -4.50 -4.58 -1.02 -1.00 3.89 0.26 -4.92 -0.95 -4.50 -2.12 -4.07
Standard name
Human Ortholog
Description Alpha-tubulin folding protein; similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance
Localization
Cell Percentages cytoplasm (73%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Alf1

Alf1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Alf1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available