Standard name
Human Ortholog
Description Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; homolog of mammalian INSIG proteins; NSG2 has a paralog, NSG1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0.06 0 0.05 0.1 0 0 0 0.06 0 0.06 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.06 0.05 0 0.06 0.05 – 0 0.05 0.09 0.07 0.06 0.11 0.16 0.22 0.14 0 0 0 0 0 0 0
Cytoplasm 0.05 0.15 0 0.09 0.08 0.21 0.1 – 0.13 0.13 0.11 0.13 0.12 0.12 0.1 0.22 0.36 0.18 0 0.06 0 0 0 0
Endoplasmic Reticulum 0.89 0.83 0.84 0.88 0.71 0.61 0.55 – 0.54 0.51 0.57 0.55 0.79 0.73 0.81 0 0 0 0.95 0.85 0.92 0.89 0.89 0.87
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0.05 0 0 – 0 0 0 0 0 0 0 0.15 0.21 0.15 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0.17 0.14 0.12 0.15 0 0 0 0.11 0.12 0.06 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Nuclear Periphery 0.07 0 0.05 0.06 0 0.05 0.07 – 0.11 0.08 0 0.06 0 0.05 0.05 0.06 0.06 0.05 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.15 0.06 0.19 0.05 0.26 0.16 0.29 – 0.18 0.24 0.19 0.16 0.12 0.13 0.08 0.29 0.18 0.46 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 4 5 5 5 4 12 – 16 11 14 37 13 15 13 7 4 6 1 2 2 4 2 4
Bud 0 0 1 0 0 0 0 – 0 4 3 2 0 1 0 0 0 0 0 0 0 1 1 2
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 3 13 6 8 10 13 – 9 14 25 26 25 53 87 26 16 3 0 0 0 1 2 3
Cytoplasm 8 24 9 11 18 36 26 – 35 37 33 48 47 57 57 26 40 17 0 11 2 8 6 12
Endoplasmic Reticulum 155 137 170 113 156 105 148 – 147 141 165 199 305 336 442 3 4 3 179 175 194 300 297 385
Endosome 0 0 1 0 2 1 1 – 1 1 0 0 1 1 1 1 0 3 0 0 0 1 1 1
Golgi 2 0 6 1 12 0 7 – 0 2 3 1 4 4 3 18 24 14 0 1 0 0 1 0
Mitochondria 1 1 0 1 1 4 10 – 45 39 34 55 3 2 0 13 13 6 0 1 5 7 4 9
Nucleus 2 0 0 0 1 0 3 – 2 7 2 1 1 0 0 3 1 5 0 0 0 0 0 0
Nuclear Periphery 12 5 11 8 7 8 20 – 29 23 7 20 15 22 25 7 7 5 2 0 2 1 2 1
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane 26 10 38 6 57 28 79 – 49 68 55 56 48 60 46 34 20 43 3 8 1 8 11 22
Unique Cell Count 174 165 202 129 220 172 271 272 278 290 360 387 461 545 118 112 93 189 206 212 339 335 445
Labelled Cell Count 208 184 254 151 267 196 319 333 347 341 446 462 551 674 139 129 105 189 206 212 339 335 445


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.9 8.6 12.1 12.5 14.8 13.6 12.6 – 11.3 11.7 12.0 11.8 11.5 12.1 11.7 12.8 14.6 14.3 11.6 12.0 11.9
Std Deviation (1e-4) 2.0 1.6 2.0 2.1 2.8 3.4 2.5 – 2.8 2.8 2.9 2.7 2.0 2.0 2.0 3.3 3.7 4.4 1.9 1.9 1.9
Intensity Change (Log2) – – – 0.04 0.29 0.17 0.06 – -0.1 -0.05 -0.01 -0.04 -0.07 -0.0 -0.05 0.08 0.27 0.24 -0.07 -0.02 -0.03

WT3RAP60RAP140RAP220RAP300RAP460RAP540RAP620RAP700051015WT3HU80HU120HU160051015WT3rpd3Δ_1rpd3Δ_2rpd3Δ_3051015WT1AF100AF140AF180051015
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 1.1 – 1.8 0.9 1.3 3.4 0.6 0.5 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.7 -1.3 -0.2 -0.8 – -1.6 -0.7 0.9 0.4 0 2.0 3.4 4.1 2.3 -1.1
Cytoplasm 1.5 1.6 4.9 2.1 – 3.1 3.3 2.7 3.3 3.0 3.1 2.6 4.9 7.3 3.9
Endoplasmic Reticulum 0.9 -3.2 -5.0 -6.8 – -6.9 -7.6 -6.4 -6.9 -1.6 -3.1 -1.0 -14.1 -13.8 -13.1
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 1.3 0 -0.3 – 0 0 0 0 0 0 0 4.0 5.3 3.8
Mitochondria 0 0 0 0 – 6.1 5.6 5.0 5.8 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.3 -1.1 -0.3 0.8 – 2.0 1.2 -1.8 0.1 -0.9 -0.4 -0.5 0.2 0.3 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole -3.7 1.7 -0.6 2.6 – -0.2 1.5 0 -1.0 -2.1 -1.9 -4.0 2.1 -0.2 4.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.6246 9.3105 9.4479 9.0144 8.6689 9.1988 8.943 9.2635 8.8028 8.4598 7.6522 8.8651 7.0832 7.1181 6.6433 6.5987 6.4184 6.6113
Actin 0.0296 0.0001 0.0038 0.0175 0.0063 0.0003 0.0121 0.0002 0.0078 0 0.0001 0.0004 0.0183 0.0014 0.0008 0 0.0002 0.0025
Bud 0.0002 0 0.0001 0.0001 0.0005 0 0 0 0 0 0 0 0.0001 0.0001 0 0 0 0.0001
Bud Neck 0.0003 0 0.0003 0.0003 0.0006 0.0007 0.0001 0.0002 0.0013 0 0.0001 0.0002 0.0116 0.0002 0.0014 0 0.0004 0.0008
Bud Periphery 0.0003 0 0.0002 0.0002 0.0007 0 0.0001 0 0.0001 0 0 0.0001 0.0002 0.0001 0.0002 0 0 0.0003
Bud Site 0.0006 0 0.0003 0.0001 0.0024 0 0.0001 0.0001 0.0003 0 0 0 0.0015 0.0023 0.0003 0 0.0001 0
Cell Periphery 0.0005 0.0004 0.0029 0.001 0.0003 0.0002 0.0003 0.0008 0.0005 0.0002 0.0003 0.0003 0.0005 0.0008 0.0026 0.0002 0.0002 0.0003
Cytoplasm 0.0258 0.0133 0.0393 0.018 0.0156 0.0248 0.0081 0.0328 0.0186 0.001 0.0097 0.0136 0.031 0.0729 0.0334 0.0129 0.0186 0.0284
Cytoplasmic Foci 0.0126 0.0005 0.0012 0.004 0.0144 0.0005 0.0019 0.0007 0.0028 0 0.0002 0.0012 0.0037 0.003 0.0012 0.0005 0.0015 0.001
Eisosomes 0.0003 0 0.0003 0.0001 0.0001 0 0.0002 0 0.0001 0 0 0 0.0003 0 0 0 0 0
Endoplasmic Reticulum 0.7607 0.9688 0.8947 0.904 0.8488 0.9436 0.9219 0.9144 0.9387 0.9925 0.9673 0.9233 0.8488 0.8767 0.9375 0.9612 0.9365 0.9302
Endosome 0.0299 0.0025 0.0082 0.0075 0.0255 0.0048 0.0067 0.0053 0.0069 0.0008 0.0036 0.0135 0.0103 0.0048 0.0026 0.0032 0.0141 0.0049
Golgi 0.0123 0.0003 0.0035 0.0051 0.0092 0.0002 0.0038 0.0002 0.0022 0.0001 0.0004 0.0011 0.0029 0.0011 0.0004 0.0001 0.0004 0.0021
Lipid Particles 0.0276 0.0019 0.0053 0.011 0.0094 0.0007 0.0042 0.0014 0.0052 0.0001 0.001 0.0026 0.0096 0.0056 0.0022 0.0014 0.0035 0.0017
Mitochondria 0.0193 0 0.0022 0.001 0.0064 0.0002 0.0006 0.0001 0.0005 0.0001 0.0003 0.0027 0.0014 0.0002 0.0001 0.0001 0.0002 0.0011
None 0.0071 0.0003 0.0117 0.0159 0.0078 0.0027 0.0007 0.0158 0.0007 0 0.0002 0.0087 0.005 0.0065 0.0012 0.0002 0.0003 0.0006
Nuclear Periphery 0.0437 0.0093 0.0181 0.0076 0.023 0.0156 0.0283 0.02 0.0095 0.004 0.0126 0.0249 0.0324 0.0163 0.012 0.0168 0.0135 0.0175
Nucleolus 0.0003 0 0.0001 0.0001 0.0003 0 0 0.0001 0.0001 0 0 0.0001 0.0009 0 0 0 0.0001 0.0001
Nucleus 0.0075 0.0005 0.0014 0.0005 0.0042 0.0019 0.0011 0.0029 0.0007 0.0001 0.0011 0.0019 0.0101 0.0027 0.0011 0.0011 0.0025 0.0035
Peroxisomes 0.0107 0 0.0002 0.0007 0.0063 0 0.007 0 0.0005 0 0 0.0001 0.0034 0.0001 0 0 0 0.0009
Punctate Nuclear 0.0051 0.0001 0.0016 0.0013 0.0144 0.0001 0.0006 0.0002 0.0012 0 0.0001 0.0007 0.0039 0.0005 0.0003 0 0.0009 0.0011
Vacuole 0.0034 0.0017 0.0035 0.0035 0.0028 0.0032 0.0015 0.0043 0.0018 0.0009 0.0025 0.0034 0.0031 0.0044 0.0024 0.0019 0.0066 0.0026
Vacuole Periphery 0.0024 0.0002 0.0012 0.0004 0.0012 0.0005 0.0007 0.0005 0.0005 0.0002 0.0004 0.0012 0.0008 0.0004 0.0002 0.0003 0.0004 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 31.2631 30.6873 37.4099 48.2989 20.6644 44.1117 44.0485 39.2657 51.1433 39.8905
Translational Efficiency 0.9045 0.8743 0.7812 0.714 1.1853 0.8921 0.7333 1.1673 0.7951 0.789

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2300 1342 1531 866 1818 1783 3018 1380 4118 3125 4549 2246

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1055.47 1051.25 1138.72 1263.56 977.84 1157.08 1183.98 1288.32 1021.20 1111.63 1168.75 1278.77
Standard Deviation 161.26 193.48 165.45 184.78 149.37 162.54 159.45 201.04 160.81 184.10 162.90 195.30
Intensity Change Log 2 — -0.005780 0.109527 0.259609 — 0.242818 0.275974 0.397821 — 0.119126 0.191973 0.327730

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000599 0.001122 0.000912 0.002595 0.000454 0.000302 0.001272 0.002685 0.000535 0.000654 0.001151 0.002650
Bud Neck 0.000227 0.000530 0.000853 0.004096 0.000206 0.000264 0.001704 0.004199 0.000218 0.000378 0.001418 0.004159
Bud Site 0.002590 0.006571 0.004369 0.014833 0.002741 0.001172 0.005021 0.013434 0.002656 0.003491 0.004802 0.013974
Cell Periphery 0.000973 0.001319 0.001009 0.003919 0.000703 0.001076 0.000880 0.003519 0.000854 0.001180 0.000924 0.003673
Cytoplasm 0.028870 0.037710 0.027088 0.029505 0.029181 0.022415 0.055856 0.023913 0.029007 0.028983 0.046174 0.026070
Cytoplasmic Foci 0.006100 0.020965 0.001422 0.005365 0.008228 0.003734 0.004209 0.004381 0.007040 0.011134 0.003271 0.004761
Eisosomes 0.000211 0.000095 0.000078 0.000164 0.000066 0.000118 0.000264 0.000146 0.000147 0.000108 0.000202 0.000153
Endoplasmic Reticulum 0.928060 0.853408 0.936822 0.837303 0.921443 0.944759 0.865512 0.863517 0.925139 0.905530 0.889512 0.853410
Endosome 0.003099 0.012634 0.003727 0.013572 0.003736 0.002578 0.004569 0.012279 0.003380 0.006896 0.004286 0.012778
Golgi 0.003982 0.011487 0.000606 0.003779 0.005407 0.004924 0.001520 0.005333 0.004611 0.007743 0.001212 0.004734
Lipid Particles 0.005124 0.010234 0.002100 0.017756 0.005912 0.004798 0.007496 0.017331 0.005472 0.007132 0.005680 0.017494
Mitochondria 0.002642 0.003352 0.000277 0.000566 0.002884 0.002279 0.001365 0.001088 0.002749 0.002740 0.000999 0.000887
Mitotic Spindle 0.000060 0.001080 0.000135 0.007184 0.000301 0.000162 0.000822 0.001887 0.000166 0.000556 0.000591 0.003929
None 0.004052 0.004323 0.000761 0.000692 0.002363 0.002653 0.003011 0.000905 0.003306 0.003370 0.002254 0.000823
Nuclear Periphery 0.001377 0.003087 0.002096 0.002079 0.002788 0.000704 0.007866 0.001931 0.002000 0.001727 0.005924 0.001988
Nuclear Periphery Foci 0.006030 0.017700 0.010615 0.034564 0.007973 0.003272 0.018432 0.026293 0.006888 0.009468 0.015801 0.029482
Nucleolus 0.000038 0.000166 0.000009 0.000032 0.000085 0.000144 0.000056 0.000047 0.000059 0.000154 0.000041 0.000041
Nucleus 0.001395 0.001755 0.002676 0.002335 0.001393 0.001673 0.009737 0.002352 0.001394 0.001708 0.007361 0.002346
Peroxisomes 0.000975 0.001247 0.000111 0.000266 0.000634 0.000877 0.000322 0.000275 0.000825 0.001036 0.000251 0.000272
Vacuole 0.002389 0.008406 0.004181 0.017368 0.001944 0.001388 0.009706 0.012650 0.002192 0.004402 0.007847 0.014469
Vacuole Periphery 0.001206 0.002811 0.000152 0.002027 0.001558 0.000708 0.000378 0.001831 0.001361 0.001611 0.000302 0.001907

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.69 -0.94 -2.76 -2.10 -2.32 1.52 -3.98 -2.85 -3.05 -1.75 -0.73 -3.09 -3.77 -3.62 -2.64
Bud Neck -3.36 -3.62 -4.26 -3.96 -3.58 -0.40 -4.86 -5.66 -5.53 -3.49 -1.83 -5.66 -7.07 -6.78 -4.86
Bud Site -3.26 -1.61 -5.38 -3.54 -4.39 2.39 -2.69 -6.11 -7.16 -4.67 -1.32 -3.24 -8.17 -7.46 -6.39
Cell Periphery -1.67 -0.28 -4.02 -3.51 -3.92 -2.37 -1.50 -7.13 -6.01 -6.64 -2.50 -0.73 -7.59 -6.61 -7.36
Cytoplasm -2.93 0.42 -1.01 1.57 -1.26 2.68 -9.71 1.62 -0.89 11.17 -0.04 -9.08 0.74 0.72 8.79
Cytoplasmic Foci -6.91 5.34 0.21 6.95 -4.72 3.94 3.52 3.22 -0.99 -0.20 -3.68 5.11 2.48 5.79 -2.77
Eisosomes 1.75 1.93 0.60 -1.68 -1.91 -2.16 -3.85 -3.59 -1.06 2.08 1.00 -1.12 -0.24 -1.97 1.15
Endoplasmic Reticulum 9.70 -1.29 10.08 2.28 10.60 -4.20 9.90 8.05 11.35 0.69 4.37 9.30 12.92 9.02 6.45
Endosome -11.47 -1.84 -8.37 -0.78 -7.75 2.88 -2.36 -10.25 -11.37 -9.04 -8.17 -3.53 -13.40 -7.65 -11.81
Golgi -5.36 6.40 0.19 4.78 -3.29 0.44 5.27 0.08 -0.26 -3.10 -3.58 7.51 -0.11 2.70 -4.18
Lipid Particles -4.31 4.64 -6.18 -3.50 -7.63 0.99 -1.67 -5.88 -6.57 -5.12 -2.20 -0.59 -8.56 -7.19 -8.25
Mitochondria -1.01 6.24 5.18 4.52 -2.32 0.73 2.62 2.90 1.74 0.89 0.02 5.15 5.08 3.93 0.49
Mitotic Spindle -1.87 -1.21 -3.74 -3.18 -3.68 0.95 -1.71 -2.77 -2.98 -1.63 -1.58 -2.13 -4.58 -4.04 -3.96
None -0.25 4.52 4.52 3.98 0.27 -0.31 -0.77 2.08 2.34 3.54 -0.09 1.75 4.77 4.39 3.54
Nuclear Periphery -2.92 -2.02 -1.95 1.46 -0.03 3.98 -6.09 1.35 -4.57 8.28 0.78 -7.63 -0.17 -1.02 7.82
Nuclear Periphery Foci -6.09 -3.53 -8.36 -4.58 -6.84 4.87 -7.12 -7.22 -9.44 -3.10 -2.66 -8.70 -11.12 -9.56 -6.51
Nucleolus -2.72 2.47 -0.48 2.46 -2.28 -0.49 0.64 0.86 0.79 0.56 -1.30 0.81 0.46 1.46 -0.42
Nucleus -1.30 -2.70 -2.80 -2.22 -0.45 -0.69 -11.49 -2.79 -1.75 8.25 -1.19 -11.65 -3.96 -2.78 6.95
Peroxisomes -1.12 5.08 3.92 4.81 -2.27 -1.53 3.45 3.86 4.06 0.89 -1.44 5.51 5.15 6.29 -0.42
Vacuole -5.71 -2.93 -8.67 -4.84 -7.13 1.63 -10.20 -7.46 -7.79 -2.03 -4.45 -10.25 -11.06 -8.75 -5.49
Vacuole Periphery -3.73 3.96 -0.89 0.86 -2.14 1.82 2.87 -0.42 -1.95 -2.72 -0.80 4.46 -1.05 -0.59 -3.41
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; homolog of mammalian INSIG proteins; NSG2 has a paralog, NSG1, that arose from the whole genome duplication
Localization
Cell Percentages ER (99%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Nsg2

Nsg2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nsg2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available