Standard name | |
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Human Ortholog | |
Description | Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; homolog of mammalian INSIG proteins; NSG2 has a paralog, NSG1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 4 | 5 | 5 | 5 | 4 | 12 | – | 16 | 11 | 14 | 37 | 13 | 15 | 13 | 7 | 4 | 6 | 1 | 2 | 2 | 4 | 2 | 4 |
Bud | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 4 | 3 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 3 | 13 | 6 | 8 | 10 | 13 | – | 9 | 14 | 25 | 26 | 25 | 53 | 87 | 26 | 16 | 3 | 0 | 0 | 0 | 1 | 2 | 3 |
Cytoplasm | 8 | 24 | 9 | 11 | 18 | 36 | 26 | – | 35 | 37 | 33 | 48 | 47 | 57 | 57 | 26 | 40 | 17 | 0 | 11 | 2 | 8 | 6 | 12 |
Endoplasmic Reticulum | 155 | 137 | 170 | 113 | 156 | 105 | 148 | – | 147 | 141 | 165 | 199 | 305 | 336 | 442 | 3 | 4 | 3 | 179 | 175 | 194 | 300 | 297 | 385 |
Endosome | 0 | 0 | 1 | 0 | 2 | 1 | 1 | – | 1 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 3 | 0 | 0 | 0 | 1 | 1 | 1 |
Golgi | 2 | 0 | 6 | 1 | 12 | 0 | 7 | – | 0 | 2 | 3 | 1 | 4 | 4 | 3 | 18 | 24 | 14 | 0 | 1 | 0 | 0 | 1 | 0 |
Mitochondria | 1 | 1 | 0 | 1 | 1 | 4 | 10 | – | 45 | 39 | 34 | 55 | 3 | 2 | 0 | 13 | 13 | 6 | 0 | 1 | 5 | 7 | 4 | 9 |
Nucleus | 2 | 0 | 0 | 0 | 1 | 0 | 3 | – | 2 | 7 | 2 | 1 | 1 | 0 | 0 | 3 | 1 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 12 | 5 | 11 | 8 | 7 | 8 | 20 | – | 29 | 23 | 7 | 20 | 15 | 22 | 25 | 7 | 7 | 5 | 2 | 0 | 2 | 1 | 2 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 26 | 10 | 38 | 6 | 57 | 28 | 79 | – | 49 | 68 | 55 | 56 | 48 | 60 | 46 | 34 | 20 | 43 | 3 | 8 | 1 | 8 | 11 | 22 |
Unique Cell Count | 174 | 165 | 202 | 129 | 220 | 172 | 271 | 272 | 278 | 290 | 360 | 387 | 461 | 545 | 118 | 112 | 93 | 189 | 206 | 212 | 339 | 335 | 445 | |
Labelled Cell Count | 208 | 184 | 254 | 151 | 267 | 196 | 319 | 333 | 347 | 341 | 446 | 462 | 551 | 674 | 139 | 129 | 105 | 189 | 206 | 212 | 339 | 335 | 445 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.9 | 8.6 | 12.1 | 12.5 | 14.8 | 13.6 | 12.6 | – | 11.3 | 11.7 | 12.0 | 11.8 | 11.5 | 12.1 | 11.7 | 12.8 | 14.6 | 14.3 | 11.6 | 12.0 | 11.9 |
Std Deviation (1e-4) | 2.0 | 1.6 | 2.0 | 2.1 | 2.8 | 3.4 | 2.5 | – | 2.8 | 2.8 | 2.9 | 2.7 | 2.0 | 2.0 | 2.0 | 3.3 | 3.7 | 4.4 | 1.9 | 1.9 | 1.9 |
Intensity Change (Log2) | – | – | – | 0.04 | 0.29 | 0.17 | 0.06 | – | -0.1 | -0.05 | -0.01 | -0.04 | -0.07 | -0.0 | -0.05 | 0.08 | 0.27 | 0.24 | -0.07 | -0.02 | -0.03 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 1.1 | – | 1.8 | 0.9 | 1.3 | 3.4 | 0.6 | 0.5 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.7 | -1.3 | -0.2 | -0.8 | – | -1.6 | -0.7 | 0.9 | 0.4 | 0 | 2.0 | 3.4 | 4.1 | 2.3 | -1.1 |
Cytoplasm | 1.5 | 1.6 | 4.9 | 2.1 | – | 3.1 | 3.3 | 2.7 | 3.3 | 3.0 | 3.1 | 2.6 | 4.9 | 7.3 | 3.9 |
Endoplasmic Reticulum | 0.9 | -3.2 | -5.0 | -6.8 | – | -6.9 | -7.6 | -6.4 | -6.9 | -1.6 | -3.1 | -1.0 | -14.1 | -13.8 | -13.1 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 1.3 | 0 | -0.3 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.0 | 5.3 | 3.8 |
Mitochondria | 0 | 0 | 0 | 0 | – | 6.1 | 5.6 | 5.0 | 5.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0.3 | -1.1 | -0.3 | 0.8 | – | 2.0 | 1.2 | -1.8 | 0.1 | -0.9 | -0.4 | -0.5 | 0.2 | 0.3 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -3.7 | 1.7 | -0.6 | 2.6 | – | -0.2 | 1.5 | 0 | -1.0 | -2.1 | -1.9 | -4.0 | 2.1 | -0.2 | 4.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 9.6246 | 9.3105 | 9.4479 | 9.0144 | 8.6689 | 9.1988 | 8.943 | 9.2635 | 8.8028 | 8.4598 | 7.6522 | 8.8651 | 7.0832 | 7.1181 | 6.6433 | 6.5987 | 6.4184 | 6.6113 |
Actin | 0.0296 | 0.0001 | 0.0038 | 0.0175 | 0.0063 | 0.0003 | 0.0121 | 0.0002 | 0.0078 | 0 | 0.0001 | 0.0004 | 0.0183 | 0.0014 | 0.0008 | 0 | 0.0002 | 0.0025 |
Bud | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0005 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 |
Bud Neck | 0.0003 | 0 | 0.0003 | 0.0003 | 0.0006 | 0.0007 | 0.0001 | 0.0002 | 0.0013 | 0 | 0.0001 | 0.0002 | 0.0116 | 0.0002 | 0.0014 | 0 | 0.0004 | 0.0008 |
Bud Periphery | 0.0003 | 0 | 0.0002 | 0.0002 | 0.0007 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0 | 0 | 0.0003 |
Bud Site | 0.0006 | 0 | 0.0003 | 0.0001 | 0.0024 | 0 | 0.0001 | 0.0001 | 0.0003 | 0 | 0 | 0 | 0.0015 | 0.0023 | 0.0003 | 0 | 0.0001 | 0 |
Cell Periphery | 0.0005 | 0.0004 | 0.0029 | 0.001 | 0.0003 | 0.0002 | 0.0003 | 0.0008 | 0.0005 | 0.0002 | 0.0003 | 0.0003 | 0.0005 | 0.0008 | 0.0026 | 0.0002 | 0.0002 | 0.0003 |
Cytoplasm | 0.0258 | 0.0133 | 0.0393 | 0.018 | 0.0156 | 0.0248 | 0.0081 | 0.0328 | 0.0186 | 0.001 | 0.0097 | 0.0136 | 0.031 | 0.0729 | 0.0334 | 0.0129 | 0.0186 | 0.0284 |
Cytoplasmic Foci | 0.0126 | 0.0005 | 0.0012 | 0.004 | 0.0144 | 0.0005 | 0.0019 | 0.0007 | 0.0028 | 0 | 0.0002 | 0.0012 | 0.0037 | 0.003 | 0.0012 | 0.0005 | 0.0015 | 0.001 |
Eisosomes | 0.0003 | 0 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0.0003 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.7607 | 0.9688 | 0.8947 | 0.904 | 0.8488 | 0.9436 | 0.9219 | 0.9144 | 0.9387 | 0.9925 | 0.9673 | 0.9233 | 0.8488 | 0.8767 | 0.9375 | 0.9612 | 0.9365 | 0.9302 |
Endosome | 0.0299 | 0.0025 | 0.0082 | 0.0075 | 0.0255 | 0.0048 | 0.0067 | 0.0053 | 0.0069 | 0.0008 | 0.0036 | 0.0135 | 0.0103 | 0.0048 | 0.0026 | 0.0032 | 0.0141 | 0.0049 |
Golgi | 0.0123 | 0.0003 | 0.0035 | 0.0051 | 0.0092 | 0.0002 | 0.0038 | 0.0002 | 0.0022 | 0.0001 | 0.0004 | 0.0011 | 0.0029 | 0.0011 | 0.0004 | 0.0001 | 0.0004 | 0.0021 |
Lipid Particles | 0.0276 | 0.0019 | 0.0053 | 0.011 | 0.0094 | 0.0007 | 0.0042 | 0.0014 | 0.0052 | 0.0001 | 0.001 | 0.0026 | 0.0096 | 0.0056 | 0.0022 | 0.0014 | 0.0035 | 0.0017 |
Mitochondria | 0.0193 | 0 | 0.0022 | 0.001 | 0.0064 | 0.0002 | 0.0006 | 0.0001 | 0.0005 | 0.0001 | 0.0003 | 0.0027 | 0.0014 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0011 |
None | 0.0071 | 0.0003 | 0.0117 | 0.0159 | 0.0078 | 0.0027 | 0.0007 | 0.0158 | 0.0007 | 0 | 0.0002 | 0.0087 | 0.005 | 0.0065 | 0.0012 | 0.0002 | 0.0003 | 0.0006 |
Nuclear Periphery | 0.0437 | 0.0093 | 0.0181 | 0.0076 | 0.023 | 0.0156 | 0.0283 | 0.02 | 0.0095 | 0.004 | 0.0126 | 0.0249 | 0.0324 | 0.0163 | 0.012 | 0.0168 | 0.0135 | 0.0175 |
Nucleolus | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0003 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0009 | 0 | 0 | 0 | 0.0001 | 0.0001 |
Nucleus | 0.0075 | 0.0005 | 0.0014 | 0.0005 | 0.0042 | 0.0019 | 0.0011 | 0.0029 | 0.0007 | 0.0001 | 0.0011 | 0.0019 | 0.0101 | 0.0027 | 0.0011 | 0.0011 | 0.0025 | 0.0035 |
Peroxisomes | 0.0107 | 0 | 0.0002 | 0.0007 | 0.0063 | 0 | 0.007 | 0 | 0.0005 | 0 | 0 | 0.0001 | 0.0034 | 0.0001 | 0 | 0 | 0 | 0.0009 |
Punctate Nuclear | 0.0051 | 0.0001 | 0.0016 | 0.0013 | 0.0144 | 0.0001 | 0.0006 | 0.0002 | 0.0012 | 0 | 0.0001 | 0.0007 | 0.0039 | 0.0005 | 0.0003 | 0 | 0.0009 | 0.0011 |
Vacuole | 0.0034 | 0.0017 | 0.0035 | 0.0035 | 0.0028 | 0.0032 | 0.0015 | 0.0043 | 0.0018 | 0.0009 | 0.0025 | 0.0034 | 0.0031 | 0.0044 | 0.0024 | 0.0019 | 0.0066 | 0.0026 |
Vacuole Periphery | 0.0024 | 0.0002 | 0.0012 | 0.0004 | 0.0012 | 0.0005 | 0.0007 | 0.0005 | 0.0005 | 0.0002 | 0.0004 | 0.0012 | 0.0008 | 0.0004 | 0.0002 | 0.0003 | 0.0004 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 31.2631 | 30.6873 | 37.4099 | 48.2989 | 20.6644 | 44.1117 | 44.0485 | 39.2657 | 51.1433 | 39.8905 |
Translational Efficiency | 0.9045 | 0.8743 | 0.7812 | 0.714 | 1.1853 | 0.8921 | 0.7333 | 1.1673 | 0.7951 | 0.789 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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